Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0003564

Physical Location & Seq

Genomic Coordinates Reverse (-)

12815451 .. 12817256

Gene Structure 1806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G011320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGATGCTCTCAAACTCTTCTGTTTTTTCCCTGTTTCTTCTTATTTTGTTATCATCTTCATCAGCAAAACCACCATCTTCAAAACAAAACAGTTTTATCCAATGCCTCTCATATCATTCTAAGGCTTCTATTCCATTCTCTAGTACCTTTTTCACTCCAAACAGTTCTGCATTCACTACCGTCCTAAATTCAACTGCACAAAACCTCAGGTGCTTGATCCCTTCAGAGCCAAAGCCAGAGTTCATTTTCACACCAACAGATGATTCCCAAGTTCAAAATGCTGTTATTTGTTCAAAACAGCTTGGAATACATCTCAGGGTCCGAAGTGGAGGGCATGATTATGAAGGTCTCTCGTATGTGTCCCAAATTGAGACACCTTTCATTGTTGTAGACCTCGCCAAGCTTCGATCAGTTAATGTTGATATCCAAGGCAATAGTGCATGGATTCAAGCTGGAGCCACTATTGGTGAAGTTTACTACAGAATTGCAGAGAAAAGCAGAACCCACGGCTTCCCAGCTGGTCTTTGCACAAGTTTAGGGGTGGGTGGCCATATAACTGGAGGTGCATATGGTACTATGATGAGGAAATATGGCCTTGCAGCTGATAATGTCCTGGATGCCCGAATAGTTGATGTCAAAGGCAGAATTCTTGACCGAAAGACCATGGGAGAAGACCTGTTTTGGGCAATCAGAGGTGGTGGAGGAGCAAGCTTTGGAATCATCCTGTGGTGGAAGATAAAGTTGGTTCCCGTTCCATCAACTGTGACGGTTTTTACTGTTTCCAAGACTTTGGAACAGGGTGCCACAAAGCTCCTCCATAGATGGCAACAAGTAGCTGCTACTAAGCTCGACAAAGATCTCAACATTAGAGTTATGATACAACCAACAGTAGTTGACAGCAAAAGTGGCAAGAGAACTGTCACAACTCTTTACCAAGGTCAATTCCTTGGAGGTGTTAAAAGGCTCCTCAATGTTATGCAAACAGGGTTCCCCGAACTGAATTTGACAAGAAAAGATTGTACACAAACGAGTTGGCTCAAGTCTGTGATGTACATAGCAGGTTACGCAAATGGAACTCAGCCTGAGGTTTTGCTTCAAGGAAAACCCACATTCAAGAGCTACTTCAAAGCCAAATCAGACTTTGTCAAAGACCCGATTCCTGAAACTGGGCTTGAGGGGCTATGGAAAAGGTTGTTGGAAGAAACCGCTCCTCTGATTATTTGGAACCCATATGGGGGAGTGATGAGCAAAATTTCGGAGTCAGCATTACCCTTCCCACACAGAAATGTTCTCTTCAAAATTCAGTACCTGACTACATGGCAAGCTGCAAATGAAAACGAGGCAAAGCACATAAATTGGATTAGGAAGCTCTACAACTACATGGCTCCTTATGTCACAAAGTCCCCAAGGCAAGCATATGTGAATTATAGGGATCTTGATTTGGGGATAAACAATAACAAGAACACTAGCATCACTGAAGCAAGTGCTTGGGGTAACAGGTATTTCAAGGGCAACTTTTACAGATTGATAAAGATTAAAACCAAAGTTGATCCTCATAACTTCTTCAGGCACGAGCAGAGCATCCCGCCTCTTGCGCTCCAGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000741 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0006950 GO:0006979 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009559 GO:0009561 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010197 GO:0016043 GO:0016491 GO:0016614 GO:0016616 GO:0022414 GO:0030054 GO:0030154 GO:0030312 GO:0032501 GO:0032502 GO:0044464 GO:0045551 GO:0048229 GO:0048284 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050268 GO:0050896 GO:0055044 GO:0055114 GO:0061458 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00940 ko01100 ko01110 map00940 map01100 map01110

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

535

Amino Acids

59.72

Weight (kDa)

9.66

Isoelectric Point (pI)

35.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	79 - 217	7.9e-26	FAD binding domain
BBE	PF08031	472 - 529	9.3e-20	Berberine and berberine like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30760 AT1G30760 AT2G34790 AT5G44390 AT5G44400
fragaria_vesca	FvH4_3g10920
malus_domestica	MD05G1268400.v1.1 MD05G1268500.v1.1 MD10G1244500.v1.1
prunus_persica	Prupe.4G096300_v2.0.a1 Prupe.4G096400_v2.0.a1
pyrus_communis	pycom05g25130 pycom05g25140 pycom10g20500
rosa_chinensis	RchiOBHm_Chr5g0017561 RchiOBHm_Chr5g0017591 RchiOBHm_Chr5g0017601 RchiOBHm_Chr5g0017621 RchiOBHm_Chr5g0017641
rosa_laevigata	RLG00000032298 RLG00000032301 RLG00000032302
rosa_multiflora	Rmu_sc0000346.1_g000021 Rmu_sc0000346.1_g000022 Rmu_sc0002565.1_g000007 Rmu_sc0003415.1_g000020
rosa_roxburghii	Rroxscaffold_1G00059870 Rroxscaffold_1G00059880 Rroxscaffold_1G00059890
rosa_rugosa	Rorug05G0036900 Rorug05G0037000 Rorug05G0037100
rosa_wichuraiana	Rw5G011310 Rw5G011320 Rw5G011330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1049
AccB1I	GGYRCC	1 cut(s)	800
AccBSI	CCGCTC	1 cut(s)	1208
AccI	GTMKAC	1 cut(s)	390
AciI	CCGC	2 cut(s)	1206, 1586
AclWI	GGATC	3 cut(s)	211, 1440, 1544
AcoI	YGGCCR	1 cut(s)	547
AcsI	RAATTY	5 cut(s)	187, 645, 1000, 1251, 1299
AcuI	CTGAAG	3 cut(s)	207, 1497, 1549
AfaI	GTAC	5 cut(s)	145, 574, 1021, 1052, 1307
AfiI	CCNNNNNNNGG	4 cut(s)	682, 1166, 1234, 1235
AjnI	CCWGG	2 cut(s)	612, 1599
AjuI	GAANNNNNNNTTGG	6 cut(s)	247, 279, 725, 757, 1178, 1210
Alw26I	GTCTC	2 cut(s)	353, 365
AlwI	GGATC	3 cut(s)	211, 1440, 1544
AlwNI	CAGNNNCTG	1 cut(s)	1309
AoxI	GGCC	2 cut(s)	547, 592
ApeKI	GCWGC	3 cut(s)	599, 836, 1325
ApoI	RAATTY	5 cut(s)	187, 645, 1000, 1251, 1299
AspLEI	GCGC	1 cut(s)	1597
AspS9I	GGNCC	1 cut(s)	319
AsuHPI	GGTGA	1 cut(s)	479
AvaII	GGWCC	1 cut(s)	319
AxyI	CCTNAGG	2 cut(s)	206, 1083
BalI	TGGCCA	1 cut(s)	549
BanI	GGYRCC	1 cut(s)	800
BauI	CACGAG	1 cut(s)	1571
BbsI	GAAGAC	1 cut(s)	678
BbvI	GCAGC	3 cut(s)	611, 823, 1312
BccI	CCATC	3 cut(s)	82, 763, 816
BceAI	ACGGC	1 cut(s)	523
BciT130I	CCWGG	2 cut(s)	614, 1601
BcoDI	GTCTC	2 cut(s)	353, 365
BfaI	CTAG	2 cut(s)	141, 1467
BfmI	CTRYAG	1 cut(s)	478
BfuAI	ACCTGC	1 cut(s)	1049
BglII	AGATCT	1 cut(s)	856
BisI	GCNGC	3 cut(s)	600, 837, 1326
BlsI	GCNGC	3 cut(s)	601, 838, 1327
Bme1390I	CCNGG	2 cut(s)	614, 1601
Bme18I	GGWCC	1 cut(s)	319
BmgT120I	GGNCC	1 cut(s)	319
BmiI	GGNNCC	8 cut(s)	320, 457, 747, 802, 965, 989, 1226, 1386
BmrFI	CCNGG	2 cut(s)	614, 1601
BmrI	ACTGGG	1 cut(s)	1176
BmsI	GCATC	3 cut(s)	607, 1479, 1590
BmuI	ACTGGG	1 cut(s)	1176
BpiI	GAAGAC	1 cut(s)	678
BpmI	CTGGAG	3 cut(s)	474, 579, 1583
BpuEI	CTTGAG	2 cut(s)	1022, 1193
BsaI	GGTCTC	1 cut(s)	353
BsaJI	CCNNGG	6 cut(s)	427, 505, 663, 934, 946, 1406
Bsc4I	CCNNNNNNNGG	4 cut(s)	682, 1166, 1234, 1235
Bse1I	ACTGG	2 cut(s)	562, 1171
Bse21I	CCTNAGG	2 cut(s)	206, 1083
BseBI	CCWGG	2 cut(s)	614, 1601
BseDI	CCNNGG	6 cut(s)	427, 505, 663, 934, 946, 1406
BseGI	GGATG	3 cut(s)	622, 720, 1581
BseLI	CCNNNNNNNGG	4 cut(s)	682, 1166, 1234, 1235
BseMII	CTCAG	4 cut(s)	220, 328, 1074, 1091
BseNI	ACTGG	2 cut(s)	562, 1171
BseRI	GAGGAG	4 cut(s)	717, 803, 956, 1200
BseXI	GCAGC	3 cut(s)	611, 823, 1312
BseYI	CCCAGC	1 cut(s)	514
BsgI	GTGCAG	1 cut(s)	180
BshFI	GGCC	2 cut(s)	549, 594
BshNI	GGYRCC	1 cut(s)	800
BslFI	GGGAC	2 cut(s)	346, 1387
BslI	CCNNNNNNNGG	4 cut(s)	682, 1166, 1234, 1235
BsmAI	GTCTC	2 cut(s)	353, 365
BsmFI	GGGAC	2 cut(s)	346, 1387
BsmI	GAATGC	1 cut(s)	170
BsnI	GGCC	2 cut(s)	549, 594
Bso31I	GGTCTC	1 cut(s)	353
Bsp1407I	TGTACA	2 cut(s)	1019, 1050
Bsp143I	GATC	5 cut(s)	216, 407, 856, 1432, 1549
Bsp19I	CCATGG	1 cut(s)	663
BspACI	CCGC	2 cut(s)	1206, 1586
BspANI	GGCC	2 cut(s)	549, 594
BspCNI	CTCAG	4 cut(s)	219, 327, 1075, 1090
BspLI	GGNNCC	8 cut(s)	320, 457, 747, 802, 965, 989, 1226, 1386
BspMI	ACCTGC	1 cut(s)	1049
BspPI	GGATC	3 cut(s)	211, 1440, 1544
BspT107I	GGYRCC	1 cut(s)	800
BspTNI	GGTCTC	1 cut(s)	353
BsrBI	CCGCTC	1 cut(s)	1208
BsrGI	TGTACA	2 cut(s)	1019, 1050
BsrI	ACTGG	2 cut(s)	562, 1171
BssECI	CCNNGG	6 cut(s)	427, 505, 663, 934, 946, 1406
BssMI	GATC	5 cut(s)	216, 407, 856, 1432, 1549
BssSI	CACGAG	1 cut(s)	1571
BssT1I	CCWWGG	5 cut(s)	427, 663, 934, 946, 1406
Bst2BI	CACGAG	1 cut(s)	1571
Bst2UI	CCWGG	2 cut(s)	614, 1601
Bst4CI	ACNGT	8 cut(s)	92, 164, 181, 763, 769, 778, 889, 919
Bst6I	CTCTTC	2 cut(s)	22, 1298
BstAUI	TGTACA	2 cut(s)	1019, 1050
BstC8I	GCNNGC	3 cut(s)	709, 1323, 1413
BstDEI	CTNAG	6 cut(s)	120, 206, 314, 843, 1077, 1083
BstDSI	CCRYGG	2 cut(s)	505, 663
BstF5I	GGATG	3 cut(s)	622, 720, 1581
BstHHI	GCGC	1 cut(s)	1597
BstKTI	GATC	5 cut(s)	219, 410, 859, 1435, 1552
BstMAI	GTCTC	2 cut(s)	353, 365
BstMBI	GATC	5 cut(s)	216, 407, 856, 1432, 1549
BstMWI	GCNNNNNNNGC	5 cut(s)	906, 1064, 1177, 1594, 1601
BstNI	CCWGG	2 cut(s)	614, 1601
BstSCI	CCNGG	2 cut(s)	612, 1599
BstSFI	CTRYAG	1 cut(s)	478
BstV1I	GCAGC	3 cut(s)	611, 823, 1312
BstV2I	GAAGAC	1 cut(s)	678
BstX2I	RGATCY	2 cut(s)	856, 1432
BstXI	CCANNNNNNTGG	2 cut(s)	557, 790
BstYI	RGATCY	2 cut(s)	856, 1432
Bsu36I	CCTNAGG	2 cut(s)	206, 1083
BsuRI	GGCC	2 cut(s)	549, 594
BtgI	CCRYGG	2 cut(s)	505, 663
BtsCI	GGATG	3 cut(s)	622, 720, 1581
BtsIMutI	CAGTG	1 cut(s)	1473
BveI	ACCTGC	1 cut(s)	1049
Cac8I	GCNNGC	3 cut(s)	709, 1323, 1413
CaiI	CAGNNNCTG	1 cut(s)	1309
CfoI	GCGC	1 cut(s)	1597
Cfr13I	GGNCC	1 cut(s)	319
Csp6I	GTAC	5 cut(s)	144, 573, 1020, 1051, 1306
CviAII	CATG	6 cut(s)	335, 441, 664, 1317, 1381, 1605
CviQI	GTAC	5 cut(s)	144, 573, 1020, 1051, 1306
DdeI	CTNAG	6 cut(s)	120, 206, 314, 843, 1077, 1083
DpnI	GATC	5 cut(s)	218, 409, 858, 1434, 1551
DpnII	GATC	5 cut(s)	216, 407, 856, 1432, 1549
EaeI	YGGCCR	1 cut(s)	547
Eam1104I	CTCTTC	2 cut(s)	22, 1298
EarI	CTCTTC	2 cut(s)	22, 1298
Eco130I	CCWWGG	5 cut(s)	427, 663, 934, 946, 1406
Eco31I	GGTCTC	1 cut(s)	353
Eco32I	GATATC	1 cut(s)	424
Eco47I	GGWCC	1 cut(s)	319
Eco57I	CTGAAG	3 cut(s)	207, 1497, 1549
Eco81I	CCTNAGG	2 cut(s)	206, 1083
EcoRI	GAATTC	1 cut(s)	645
EcoRII	CCWGG	2 cut(s)	612, 1599
EcoRV	GATATC	1 cut(s)	424
EcoT14I	CCWWGG	5 cut(s)	427, 663, 934, 946, 1406
ErhI	CCWWGG	5 cut(s)	427, 663, 934, 946, 1406
FaeI	CATG	6 cut(s)	338, 444, 667, 1320, 1384, 1608
FalI	AAGNNNNNCTT	4 cut(s)	1106, 1138, 1499, 1531
FaqI	GGGAC	2 cut(s)	346, 1387
FatI	CATG	6 cut(s)	334, 440, 663, 1316, 1380, 1604
FauI	CCCGC	1 cut(s)	1593
FauNDI	CATATG	3 cut(s)	568, 1231, 1417
FblI	GTMKAC	1 cut(s)	390
Fnu4HI	GCNGC	3 cut(s)	600, 837, 1326
FokI	GGATG	3 cut(s)	629, 707, 1568
Fsp4HI	GCNGC	3 cut(s)	600, 837, 1326
FspBI	CTAG	2 cut(s)	141, 1467
GlaI	GCGC	1 cut(s)	1596
GluI	GCNGC	3 cut(s)	600, 837, 1326
GsaI	CCCAGC	1 cut(s)	518
GsuI	CTGGAG	3 cut(s)	474, 579, 1583
HaeIII	GGCC	2 cut(s)	549, 594
HhaI	GCGC	1 cut(s)	1597
Hin1II	CATG	6 cut(s)	338, 444, 667, 1320, 1384, 1608
Hin6I	GCGC	1 cut(s)	1595
HinP1I	GCGC	1 cut(s)	1595
HincII	GTYRAC	1 cut(s)	895
HindII	GTYRAC	1 cut(s)	895
HindIII	AAGCTT	2 cut(s)	401, 709
HinfI	GANTC	5 cut(s)	263, 445, 717, 1156, 1259
HphI	GGTGA	1 cut(s)	479
Hpy166II	GTNNAC	4 cut(s)	391, 475, 895, 1022
Hpy188I	TCNGA	6 cut(s)	226, 323, 692, 1138, 1215, 1258
Hpy188III	TCNNGA	4 cut(s)	650, 1114, 1160, 1436
Hpy8I	GTNNAC	4 cut(s)	391, 475, 895, 1022
HpyAV	CCTTC	3 cut(s)	231, 338, 1282
HpyCH4III	ACNGT	8 cut(s)	92, 164, 181, 763, 769, 778, 889, 919
HpyCH4V	TGCA	9 cut(s)	170, 197, 440, 488, 528, 566, 599, 979, 1328
HpyF10VI	GCNNNNNNNGC	5 cut(s)	906, 1064, 1177, 1594, 1601
HpyF3I	CTNAG	6 cut(s)	120, 206, 314, 843, 1077, 1083
Hsp92II	CATG	6 cut(s)	338, 444, 667, 1320, 1384, 1608
HspAI	GCGC	1 cut(s)	1595
Kzo9I	GATC	5 cut(s)	216, 407, 856, 1432, 1549
LmnI	GCTCC	7 cut(s)	455, 704, 816, 969, 1213, 1390, 1602
Lsp1109I	GCAGC	3 cut(s)	611, 823, 1312
LweI	GCATC	3 cut(s)	607, 1479, 1590
MaeI	CTAG	2 cut(s)	141, 1467
MaeIII	GTNAC	5 cut(s)	763, 919, 1061, 1393, 1493
MalI	GATC	5 cut(s)	218, 409, 858, 1434, 1551
MbiI	CCGCTC	1 cut(s)	1208
MboI	GATC	5 cut(s)	216, 407, 856, 1432, 1549
MboII	GAAGA	9 cut(s)	9, 29, 48, 69, 683, 745, 1211, 1285, 1555
MflI	RGATCY	2 cut(s)	856, 1432
MlsI	TGGCCA	1 cut(s)	549
MluNI	TGGCCA	1 cut(s)	549
MlyI	GAGTC	1 cut(s)	1268
MmeI	TCCRAC	1 cut(s)	1176
Mox20I	TGGCCA	1 cut(s)	549
MscI	TGGCCA	1 cut(s)	549
MseI	TTAA	3 cut(s)	414, 957, 1536
MslI	CAYNNNNRTG	2 cut(s)	339, 1284
Msp20I	TGGCCA	1 cut(s)	549
MspA1I	CMGCKG	2 cut(s)	518, 602
MspR9I	CCNGG	2 cut(s)	614, 1601
Mva1269I	GAATGC	1 cut(s)	170
MvaI	CCWGG	2 cut(s)	614, 1601
MwoI	GCNNNNNNNGC	5 cut(s)	906, 1064, 1177, 1594, 1601
NcoI	CCATGG	1 cut(s)	663
NdeI	CATATG	3 cut(s)	568, 1231, 1417
NdeII	GATC	5 cut(s)	216, 407, 856, 1432, 1549
NlaIII	CATG	6 cut(s)	338, 444, 667, 1320, 1384, 1608
NlaIV	GGNNCC	8 cut(s)	320, 457, 747, 802, 965, 989, 1226, 1386
NmuCI	GTSAC	3 cut(s)	763, 919, 1393
PctI	GAATGC	1 cut(s)	170
PfeI	GAWTC	4 cut(s)	263, 445, 717, 1156
PflFI	GACNNNGTC	1 cut(s)	1142
PfoI	TCCNGGA	1 cut(s)	612
PkrI	GCNGC	3 cut(s)	601, 838, 1327
PleI	GAGTC	1 cut(s)	1267
PpsI	GAGTC	1 cut(s)	1267
Psp6I	CCWGG	2 cut(s)	612, 1599
PspFI	CCCAGC	1 cut(s)	514
PspGI	CCWGG	2 cut(s)	612, 1599
PspN4I	GGNNCC	8 cut(s)	320, 457, 747, 802, 965, 989, 1226, 1386
PspPI	GGNCC	1 cut(s)	319
PstNI	CAGNNNCTG	1 cut(s)	1309
PsuI	RGATCY	2 cut(s)	856, 1432
PsyI	GACNNNGTC	1 cut(s)	1142
PvuII	CAGCTG	2 cut(s)	518, 602
RsaI	GTAC	5 cut(s)	145, 574, 1021, 1052, 1307
RsaNI	GTAC	5 cut(s)	144, 573, 1020, 1051, 1306
RseI	CAYNNNNRTG	2 cut(s)	339, 1284
SaqAI	TTAA	3 cut(s)	414, 957, 1536
SatI	GCNGC	3 cut(s)	600, 837, 1326
Sau3AI	GATC	5 cut(s)	216, 407, 856, 1432, 1549
Sau96I	GGNCC	1 cut(s)	319
SchI	GAGTC	1 cut(s)	1268
ScrFI	CCNGG	2 cut(s)	614, 1601
SfaNI	GCATC	3 cut(s)	607, 1479, 1590
SfcI	CTRYAG	1 cut(s)	478
SinI	GGWCC	1 cut(s)	319
SmiMI	CAYNNNNRTG	2 cut(s)	339, 1284
SmlI	CTYRAG	2 cut(s)	1037, 1172
SmoI	CTYRAG	2 cut(s)	1037, 1172
SsiI	CCGC	2 cut(s)	1206, 1586
SspMI	CTAG	2 cut(s)	141, 1467
StyD4I	CCNGG	2 cut(s)	612, 1599
StyI	CCWWGG	5 cut(s)	427, 663, 934, 946, 1406
TaaI	ACNGT	8 cut(s)	92, 164, 181, 763, 769, 778, 889, 919
TaqI	TCGA	2 cut(s)	406, 849
TaqII	GACCGA	1 cut(s)	669
TatI	WGTACW	2 cut(s)	1019, 1050
TfiI	GAWTC	4 cut(s)	263, 445, 717, 1156
Tru1I	TTAA	3 cut(s)	414, 957, 1536
Tru9I	TTAA	3 cut(s)	414, 957, 1536
TscAI	CASTG	1 cut(s)	1480
TseFI	GTSAC	3 cut(s)	763, 919, 1393
TseI	GCWGC	3 cut(s)	599, 836, 1325
Tsp45I	GTSAC	3 cut(s)	763, 919, 1393
TspDTI	ATGAA	5 cut(s)	48, 232, 357, 370, 1347
TspRI	CASTG	1 cut(s)	1480
Tth111I	GACNNNGTC	1 cut(s)	1142
VpaK11BI	GGWCC	1 cut(s)	319
XapI	RAATTY	5 cut(s)	187, 645, 1000, 1251, 1299
XmiI	GTMKAC	1 cut(s)	390
XspI	CTAG	2 cut(s)	141, 1467

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.