RchiOBHm_Chr5g0017641

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003564
Physical Location & Seq
Reverse (-)
12211977 .. 12213366
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29791

Sequence Viewer

Length: 1176 bp
ATGATGCTTCCAAACACTTCAAAATTTGCCCTCTTTCTTTTCCTTCTGCTATCATCGGCTTCATGGGCATATTCCAAAGCTTCAACCCTGGACAGTTTCAGCCAATGCCTCTCCGATAATACCGAGATTTTGATACCATTCTCTAAGGCCTTCGTCACCCCAAACAATTCCACATTCCTTCCCACCCTACAGTCCACTGCACAAAACCTCCGGTACTTGACCCCTTCGGTGCCAAAGCCGGAGTTCATCTTCACTCCATTGCTGGATTCCCATGTCCAAGCTGCTGTCATTTGCTCAAAACAGCTTGGAATTCATATGAGAGTCCGAAGTGGAGGCCATGACTATGAAGCACTCTCTTATGTCTCCCAAACCGAAACACCTTTCATCATTGTAGACCTCGCCAAGCTTCGATCAGTCAACATTGACATCGAAGACAACAGTGCTTGGGTCCAAGCCGGTGCCTCCATTGGTGAAGTTTACTACAGAATTGCAGAAAAAAGCAAAACACATGGCTACCCTGCCGGACTCTGCACGAGTCTAGGTATTGGCGGGCACATTACTGGAGGGGCATATGGTACCATGATGAGGAAGTATGGCCTTGGTGCTGACAATGTCATTGATGCTCGGATTGTTGATGTTAGTGGCAGGATTCATAATCGAGAATCGATGGGAGAGGATCTGTTTTGGGCCATCAGAGGAGGTGGAGGAGCAAGCTTTGGGGTTATCCTCTGGTGGAAGATCAAGCTTGTTCCTGTTCCATCAACCGTGACAGTTTTTTCAGTTGTCAAGACCCTTGAGCAAGGCGCCACGAAGCTCCTCCATAGGTATCAACAAGTAGCTGCCACTGAGCTTGATGAAGATCTCTTCATTAGAGTTATCATACAACCAACGGTCATCGACACCAAAAGTGGTAACAAAACTGTCGCGACTATCTACCAAGCTCAGTTTCTTGGAGGCGTTGATAGACTCCTTGATGTTATGGGAACAAGTTTCCCTGAACTGGGTCTGACAAGGAAAGATTGCACACAAACAAGTTGGCTCAAATCTGTGATGTTCATAGCAGGTTACCCAAGTGGAACTCCACCTGAGGTTTTGCTTCAAGGAAAATCCACTTTCAAGAACTACTTCAAAGCCAAGGGTTATGGAAACGGTTATTCGAACAAGCCTCTCCTTTAG

Protein Analysis

391

Amino Acids

42.8

Weight (kDa)

8.84

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 81 - 218 1.2e-21 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 4 cut(s) 229, 458, 575, 803
AccI GTMKAC 1 cut(s) 393
AccII CGCG 1 cut(s) 926
AciI CCGC 1 cut(s) 549
AclWI GGATC 1 cut(s) 684
AcsI RAATTY 2 cut(s) 23, 309
AcyI GRCGYC 1 cut(s) 804
AfaI GTAC 2 cut(s) 215, 577
AfiI CCNNNNNNNGG 3 cut(s) 585, 1000, 1001
AgsI TTSAA 5 cut(s) 21, 84, 1100, 1117, 1129
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 2 cut(s) 930, 962
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 684
AoxI GGCC 4 cut(s) 147, 334, 595, 687
ApeKI GCWGC 2 cut(s) 281, 839
ApoI RAATTY 2 cut(s) 23, 309
Asp700I GAANNNNTTC 1 cut(s) 1124
Asp718I GGTACC 1 cut(s) 575
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 2 cut(s) 448, 687
AsuHPI GGTGA 2 cut(s) 148, 482
AsuII TTCGAA 1 cut(s) 1157
AvaII GGWCC 1 cut(s) 448
AxyI CCTNAGG 1 cut(s) 1086
BaeGI GKGCMC 1 cut(s) 555
BanI GGYRCC 4 cut(s) 229, 458, 575, 803
BauI CACGAG 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 268, 826
BccI CCATC 3 cut(s) 661, 698, 766
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 539
BfmI CTRYAG 2 cut(s) 188, 481
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 1052
BglII AGATCT 1 cut(s) 859
BisI GCNGC 2 cut(s) 282, 840
BlsI GCNGC 2 cut(s) 283, 841
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 448, 687
BmiI GGNNCC 5 cut(s) 231, 449, 460, 577, 805
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 1010
BmsI GCATC 1 cut(s) 610
BmuI ACTGGG 1 cut(s) 1010
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 1157
BpuEI CTTGAG 1 cut(s) 815
Bsa29I ATCGAT 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 804
BsaJI CCNNGG 3 cut(s) 87, 598, 1134
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 3 cut(s) 585, 1000, 1001
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 2 cut(s) 565, 1005
Bse21I CCTNAGG 1 cut(s) 1086
Bse3DI GCAATG 1 cut(s) 257
Bse8I GATNNNNATC 1 cut(s) 858
BseBI CCWGG 1 cut(s) 89
BseCI ATCGAT 1 cut(s) 665
BseDI CCNNGG 3 cut(s) 87, 598, 1134
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 3 cut(s) 585, 1000, 1001
BseMI GCAATG 1 cut(s) 257
BseMII CTCAG 3 cut(s) 837, 956, 1077
BseNI ACTGG 2 cut(s) 565, 1005
BseRI GAGGAG 3 cut(s) 711, 720, 806
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 2 cut(s) 268, 826
BsgI GTGCAG 2 cut(s) 183, 514
Bsh1236I CGCG 1 cut(s) 926
BshFI GGCC 4 cut(s) 149, 336, 597, 689
BshNI GGYRCC 4 cut(s) 229, 458, 575, 803
BshVI ATCGAT 1 cut(s) 665
BsiSI CCGG 4 cut(s) 211, 239, 456, 522
BslI CCNNNNNNNGG 3 cut(s) 585, 1000, 1001
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 4 cut(s) 149, 336, 597, 689
Bsp119I TTCGAA 1 cut(s) 1157
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 4 cut(s) 410, 676, 738, 859
Bsp68I TCGCGA 1 cut(s) 926
BspACI CCGC 1 cut(s) 549
BspANI GGCC 4 cut(s) 149, 336, 597, 689
BspCNI CTCAG 3 cut(s) 838, 955, 1078
BspDI ATCGAT 1 cut(s) 665
BspFNI CGCG 1 cut(s) 926
BspLI GGNNCC 5 cut(s) 231, 449, 460, 577, 805
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 1 cut(s) 684
BspT104I TTCGAA 1 cut(s) 1157
BspT107I GGYRCC 4 cut(s) 229, 458, 575, 803
BsrDI GCAATG 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 2 cut(s) 565, 1005
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 3 cut(s) 87, 598, 1134
BssMI GATC 4 cut(s) 410, 676, 738, 859
BssNI GRCGYC 1 cut(s) 804
BssSI CACGAG 1 cut(s) 532
BssT1I CCWWGG 2 cut(s) 598, 1134
Bst2BI CACGAG 1 cut(s) 532
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 8 cut(s) 95, 192, 440, 766, 772, 892, 922, 1151
Bst6I CTCTTC 1 cut(s) 869
BstACI GRCGYC 1 cut(s) 804
BstBI TTCGAA 1 cut(s) 1157
BstC8I GCNNGC 2 cut(s) 551, 712
BstDEI CTNAG 4 cut(s) 144, 846, 942, 1086
BstEII GGTNACC 1 cut(s) 1064
BstFNI CGCG 1 cut(s) 926
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 4 cut(s) 413, 679, 741, 862
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 4 cut(s) 410, 676, 738, 859
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 89
BstPI GGTNACC 1 cut(s) 1064
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 2 cut(s) 188, 481
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 926
BstV1I GCAGC 2 cut(s) 268, 826
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 676, 859
BstYI RGATCY 2 cut(s) 676, 859
Bsu15I ATCGAT 1 cut(s) 665
Bsu36I CCTNAGG 1 cut(s) 1086
BsuRI GGCC 4 cut(s) 149, 336, 597, 689
BsuTUI ATCGAT 1 cut(s) 665
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 3 cut(s) 195, 445, 843
BtuMI TCGCGA 1 cut(s) 926
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 2 cut(s) 551, 712
CfoI GCGC 1 cut(s) 806
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 2 cut(s) 448, 687
ClaI ATCGAT 1 cut(s) 665
Csp6I GTAC 2 cut(s) 214, 576
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 5 cut(s) 63, 272, 338, 509, 580
CviQI GTAC 2 cut(s) 214, 576
DdeI CTNAG 4 cut(s) 144, 846, 942, 1086
DinI GGCGCC 1 cut(s) 805
DpnI GATC 4 cut(s) 412, 678, 740, 861
DpnII GATC 4 cut(s) 410, 676, 738, 859
Eam1104I CTCTTC 1 cut(s) 869
EarI CTCTTC 1 cut(s) 869
Eco130I CCWWGG 2 cut(s) 598, 1134
Eco147I AGGCCT 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 448
Eco81I CCTNAGG 1 cut(s) 1086
Eco91I GGTNACC 1 cut(s) 1064
EcoO65I GGTNACC 1 cut(s) 1064
EcoRI GAATTC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 2 cut(s) 598, 1134
EgeI GGCGCC 1 cut(s) 805
EheI GGCGCC 1 cut(s) 805
ErhI CCWWGG 2 cut(s) 598, 1134
FaeI CATG 5 cut(s) 66, 275, 341, 512, 583
FalI AAGNNNNNCTT 2 cut(s) 1109, 1141
FatI CATG 5 cut(s) 62, 271, 337, 508, 579
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 2 cut(s) 315, 571
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 282, 840
Fsp4HI GCNGC 2 cut(s) 282, 840
FspBI CTAG 1 cut(s) 539
GlaI GCGC 1 cut(s) 805
GluI GCNGC 2 cut(s) 282, 840
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 4 cut(s) 149, 336, 597, 689
HapII CCGG 4 cut(s) 211, 239, 456, 522
HhaI GCGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 5 cut(s) 66, 275, 341, 512, 583
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 4 cut(s) 78, 404, 712, 743
HinfI GANTC 7 cut(s) 266, 321, 525, 535, 649, 662, 966
HpaII CCGG 4 cut(s) 211, 239, 456, 522
HphI GGTGA 2 cut(s) 148, 482
Hpy166II GTNNAC 4 cut(s) 195, 394, 418, 478
Hpy188I TCNGA 5 cut(s) 115, 326, 627, 695, 1008
Hpy188III TCNNGA 4 cut(s) 659, 787, 925, 1117
Hpy8I GTNNAC 4 cut(s) 195, 394, 418, 478
HpyAV CCTTC 4 cut(s) 53, 160, 188, 234
HpyCH4III ACNGT 8 cut(s) 95, 192, 440, 766, 772, 892, 922, 1151
HpyCH4V TGCA 4 cut(s) 200, 491, 531, 1023
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 4 cut(s) 144, 846, 942, 1086
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 5 cut(s) 66, 275, 341, 512, 583
HspAI GCGC 1 cut(s) 804
KasI GGCGCC 1 cut(s) 803
KpnI GGTACC 1 cut(s) 579
Kzo9I GATC 4 cut(s) 410, 676, 738, 859
LmnI GCTCC 2 cut(s) 707, 819
Lsp1109I GCAGC 2 cut(s) 268, 826
LweI GCATC 1 cut(s) 610
MaeI CTAG 1 cut(s) 539
MaeIII GTNAC 4 cut(s) 154, 766, 911, 1064
MalI GATC 4 cut(s) 412, 678, 740, 861
MboI GATC 4 cut(s) 410, 676, 738, 859
MboII GAAGA 5 cut(s) 241, 443, 748, 856, 869
MflI RGATCY 2 cut(s) 676, 859
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 4 cut(s) 23, 166, 309, 486
Mly113I GGCGCC 1 cut(s) 804
MlyI GAGTC 4 cut(s) 330, 519, 544, 960
MroXI GAANNNNTTC 1 cut(s) 1124
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 4 cut(s) 211, 239, 456, 522
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 804
NdeI CATATG 2 cut(s) 315, 571
NdeII GATC 4 cut(s) 410, 676, 738, 859
NlaIII CATG 5 cut(s) 66, 275, 341, 512, 583
NlaIV GGNNCC 5 cut(s) 231, 449, 460, 577, 805
NmuCI GTSAC 2 cut(s) 154, 766
NruI TCGCGA 1 cut(s) 926
NspV TTCGAA 1 cut(s) 1157
PceI AGGCCT 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 3 cut(s) 266, 649, 662
PflFI GACNNNGTC 1 cut(s) 611
PkrI GCNGC 2 cut(s) 283, 841
PleI GAGTC 4 cut(s) 329, 519, 543, 960
PluTI GGCGCC 1 cut(s) 807
PpsI GAGTC 4 cut(s) 329, 519, 543, 960
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 1064
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 5 cut(s) 231, 449, 460, 577, 805
PspPI GGNCC 2 cut(s) 448, 687
PsuI RGATCY 2 cut(s) 676, 859
PsyI GACNNNGTC 1 cut(s) 611
RruI TCGCGA 1 cut(s) 926
RsaI GTAC 2 cut(s) 215, 577
RsaNI GTAC 2 cut(s) 214, 576
RseI CAYNNNNRTG 1 cut(s) 342
SatI GCNGC 2 cut(s) 282, 840
Sau3AI GATC 4 cut(s) 410, 676, 738, 859
Sau96I GGNCC 2 cut(s) 448, 687
SchI GAGTC 4 cut(s) 330, 519, 544, 960
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 1 cut(s) 610
SfcI CTRYAG 2 cut(s) 188, 481
SfoI GGCGCC 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 1157
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse9I AATT 4 cut(s) 23, 166, 309, 486
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 1 cut(s) 549
SspDI GGCGCC 1 cut(s) 803
SspMI CTAG 1 cut(s) 539
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 2 cut(s) 598, 1134
TaaI ACNGT 8 cut(s) 95, 192, 440, 766, 772, 892, 922, 1151
TaqI TCGA 6 cut(s) 409, 429, 658, 665, 897, 1157
TasI AATT 4 cut(s) 23, 166, 309, 486
TfiI GAWTC 3 cut(s) 266, 649, 662
TscAI CASTG 3 cut(s) 202, 445, 850
TseFI GTSAC 2 cut(s) 154, 766
TseI GCWGC 2 cut(s) 281, 839
Tsp45I GTSAC 2 cut(s) 154, 766
TspDTI ATGAA 9 cut(s) 51, 235, 302, 360, 373, 641, 856, 870, 1045
TspRI CASTG 3 cut(s) 202, 445, 850
Tth111I GACNNNGTC 1 cut(s) 611
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 2 cut(s) 23, 309
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.