Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002696

Physical Location & Seq

Genomic Coordinates Forward (+)

46520733 .. 46524953

Gene Structure 4221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1345600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGGTTGCTACAGCAGCAAAGAGAATGCTCCGAAACCCGGTTCAAAGGGGCTCGGGTCATCCCAGCCTTACCAGCAGCCGCAGACGGCTGCTCAGCCTCAGAAAATATCATCCCAACAACCCCATAACCCACCACCACCTCCCAGACCACAGCCCCAGCAGAGGGCACAGCCAGCGCCCCAACCAAGGGCACAGCACCAGCAGCCAAGCCCTCCTCCAGTAAAACCGGCGCCGCCGCCGAATAGCATTCTGGGGAAGCCCTTTGATGACATTAAGCAGTACTACACACTCGGCAAAGAACTGGGCAGGGGCCAATTCGGTATCACATACTTGTGCACTGAGAACTCCACCGGCCACAGCTACGCCTGCAAGTCGATCCTGAAACGCAAACTTGTGAGCAAAAACGACAGGGACGACATCAAGAGAGAGATTCAGATAATGCAGCATTTGTCTGGGCAGCCAAACATTGTGGAGATCAAGGGGGCTTATGAGGACAGGTACTCTGTGCATTTGGTGATGGAGCTCTGCGCCGGAGGGGAACTTTTCGATCGAATTATAGCGCAGGGACAGTATTCGGAGAGGGCTGCCGCTGCGATTCTGCGTGACATTGTGAATGTTGTGCACATTTGTCACTTCATGGGCGTGCTGCACCGTGATCTCAAGCCGGAGAATTTCTTGTTGTCTAGCAAGGATGAGAAGGCAATGCTGAAGGCAACGGATTTCGGGTTGTCTGTTTTCATTGAGGAAGGAAAGGTGTACCGTGATATAGTCGGCAGTGCTTACTATGTTGCCCCTGAAGTCTTGCGGCGTAGTTATGGAAAGGAAATAGACATTTGGAGTGCAGGAGTCATTCTGTATATTCTACTCAGTGGTGTACCTCCATTTTGGGCTGAAACTGAGAGGGGTATATTCGATGCCATATTGGAAGGAGTAATAGACTTTGATAGTCAACCATGGCCATCAATATCAGACAGTGCTAAAGATTTAGTTAGAAAGATGCTGACACAGGACCCAAGAAAGAGAATTACTTCTGCAGAAGTTCTCGAGCACCCGTGGCTCAGAGTAGGCGGAGTAGCATCAGACAAGCCAATAGATAGTGCAGTTCTCTCTAGAATGAAGCAGTTCAGGGCAATGAATAAGCTCAAGCAACTTGCTTTGAAGGTCATAGCAGAGAATCTATCTGAAGAAGAAATTAAAGGCCTTAAAACAATGTTCACCAATATGGACACTGACAAAAGTGGTACAATAACTTACGAAGAACTGAAGACAGGTTTAGCGCGTATTGGGTCACGGCTGTCAGAAGCCGAAGTTAGGCAACTCATGGATGCTGCTGATGTCGATGGAAATGGATCAATTGACTACATTGAATTTATATCTGCTACTATGCATAGACAAAGATTAGAAAGAGATGAGCACCTGTACAAAGCATTTCAGTACTTTGACAAGGATAGCAGCGGGTACATTACAAGAGATGAATTAGAGGCTGCTATGAAGGAGCATGGAATGGGTGATGACAACACCATCAGAGAGATAATTTCTGAGGTTGATGCCGATAATGATGGGAGGATCAACTATTCGGAATTCTGTGCAATGATGAGAAGTGGGGCTCAACAACCAGCAAAGCTCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009751 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0019899 GO:0019902 GO:0019903 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 Pkinase

Protein Analysis

544

Amino Acids

60.86

Weight (kDa)

6.23

Isoelectric Point (pI)

45.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	95 - 353	3.1e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	96 - 349	2.4e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	400 - 461	2.1e-12	EF-hand domain pair
EF-hand_1	PF00036	401 - 427	3.8e-06	EF hand domain
EF-hand_8	PF13833	413 - 461	4.4e-06	EF-hand domain pair
EF-hand_7	PF13499	471 - 533	9.9e-17	EF-hand domain pair
EF-hand_1	PF00036	473 - 499	1.1e-06	EF hand domain
EF-hand_6	PF13405	473 - 501	3.3e-07	EF-hand domain
EF-hand_8	PF13833	484 - 533	8.7e-09	EF-hand domain pair
EF-hand_1	PF00036	508 - 534	3.5e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61950 AT4G04710 AT4G04710 AT4G04710 AT4G04710 AT4G04720 AT4G04720 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G21940 AT4G21940 AT4G21940
fragaria_vesca	FvH4_3g02130 FvH4_3g02130 FvH4_3g02130 FvH4_3g02130 FvH4_3g02130
malus_domestica	MD05G1345600.v1.1 MD10G1321000.v1.1
prunus_persica	Prupe.4G021300_v2.0.a1
pyrus_communis	pycom11g19140
rosa_chinensis	RchiOBHm_Chr5g0003201
rosa_laevigata	RLG00000031086
rosa_multiflora	Rmu_co8095158.1_g000001 Rmu_co8435131.1_g000001 Rmu_sc0000146.1_g000036
rosa_roxburghii	Rroxscaffold_1G00072810
rosa_rugosa	Rorug04G0401400
rosa_samantha	Rh5AG026300 Rh5BG027500 Rh5CG029100 Rh5DG025900
rosa_wichuraiana	Rw0G023540 Rw5G002430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	229
AccII	CGCG	1 cut(s)	1279
AciI	CCGC	7 cut(s)	80, 233, 236, 588, 805, 1068, 1455
AclWI	GGATC	3 cut(s)	370, 1357, 1574
AcoI	YGGCCR	2 cut(s)	352, 956
AcsI	RAATTY	3 cut(s)	670, 1367, 1580
AcuI	CTGAAG	4 cut(s)	728, 816, 1203, 1283
AcyI	GRCGYC	1 cut(s)	230
AfaI	GTAC	8 cut(s)	281, 500, 758, 876, 1243, 1421, 1436, 1460
AfiI	CCNNNNNNNGG	6 cut(s)	38, 162, 163, 186, 187, 1311
AgsI	TTSAA	3 cut(s)	45, 1159, 1367
AluBI	AGCT	4 cut(s)	360, 523, 1141, 1624
AluI	AGCT	4 cut(s)	360, 523, 1141, 1624
Alw21I	GWGCWC	5 cut(s)	338, 525, 624, 1050, 1416
Alw44I	GTGCAC	2 cut(s)	334, 620
AlwI	GGATC	3 cut(s)	370, 1357, 1574
Ama87I	CYCGRG	2 cut(s)	53, 1043
AoxI	GGCC	4 cut(s)	310, 352, 956, 1198
ApaLI	GTGCAC	2 cut(s)	334, 620
ApoI	RAATTY	3 cut(s)	670, 1367, 1580
Asp700I	GAANNNNTTC	2 cut(s)	1027, 1121
AspLEI	GCGC	5 cut(s)	178, 232, 530, 562, 1279
AspS9I	GGNCC	2 cut(s)	310, 1009
AsuC2I	CCSGG	1 cut(s)	39
AsuHPI	GGTGA	3 cut(s)	526, 1207, 1520
AvaI	CYCGRG	2 cut(s)	53, 1043
AvaII	GGWCC	1 cut(s)	1009
BaeGI	GKGCMC	4 cut(s)	169, 193, 338, 624
BalI	TGGCCA	1 cut(s)	958
BanI	GGYRCC	1 cut(s)	229
BanII	GRGCYC	3 cut(s)	54, 525, 1609
BbsI	GAAGAC	1 cut(s)	1271
Bbv12I	GWGCWC	5 cut(s)	338, 525, 624, 1050, 1416
BccI	CCATC	5 cut(s)	511, 967, 1334, 1529, 1553
BceAI	ACGGC	2 cut(s)	102, 1307
BcnI	CCSGG	1 cut(s)	39
BfaI	CTAG	2 cut(s)	684, 1110
BfmI	CTRYAG	2 cut(s)	10, 1032
BfoI	RGCGCY	2 cut(s)	179, 233
BlpI	GCTNAGC	1 cut(s)	93
BmcAI	AGTACT	2 cut(s)	281, 1436
Bme1390I	CCNGG	1 cut(s)	39
Bme18I	GGWCC	1 cut(s)	1009
BmeT110I	CYCGRG	2 cut(s)	53, 1043
BmgT120I	GGNCC	2 cut(s)	310, 1009
BmiI	GGNNCC	3 cut(s)	231, 311, 1011
BmrFI	CCNGG	1 cut(s)	39
BmrI	ACTGGG	1 cut(s)	311
BmsI	GCATC	5 cut(s)	904, 987, 1085, 1315, 1537
BmuI	ACTGGG	1 cut(s)	311
BpiI	GAAGAC	1 cut(s)	1271
BpmI	CTGGAG	1 cut(s)	201
Bpu1102I	GCTNAGC	1 cut(s)	93
BpuEI	CTTGAG	2 cut(s)	644, 1127
BpuMI	CCSGG	1 cut(s)	39
BsaHI	GRCGYC	1 cut(s)	230
BsaJI	CCNNGG	3 cut(s)	185, 953, 1052
Bsc4I	CCNNNNNNNGG	6 cut(s)	38, 162, 163, 186, 187, 1311
Bse118I	RCCGGY	2 cut(s)	226, 350
Bse1I	ACTGG	2 cut(s)	218, 306
Bse3DI	GCAATG	3 cut(s)	708, 1137, 1596
BseDI	CCNNGG	3 cut(s)	185, 953, 1052
BseGI	GGATG	4 cut(s)	59, 110, 697, 1330
BseLI	CCNNNNNNNGG	6 cut(s)	38, 162, 163, 186, 187, 1311
BseMI	GCAATG	3 cut(s)	708, 1137, 1596
BseMII	CTCAG	7 cut(s)	107, 113, 330, 880, 888, 1072, 1530
BseNI	ACTGG	2 cut(s)	218, 306
BseRI	GAGGAG	1 cut(s)	204
BseSI	GKGCMC	4 cut(s)	169, 193, 338, 624
BseYI	CCCAGC	2 cut(s)	63, 156
BsgI	GTGCAG	3 cut(s)	632, 861, 1119
Bsh1236I	CGCG	1 cut(s)	1279
Bsh1285I	CGRYCG	1 cut(s)	550
BshFI	GGCC	4 cut(s)	312, 354, 958, 1200
BshNI	GGYRCC	1 cut(s)	229
BsiEI	CGRYCG	1 cut(s)	550
BsiHKAI	GWGCWC	5 cut(s)	338, 525, 624, 1050, 1416
BsiHKCI	CYCGRG	2 cut(s)	53, 1043
BsiSI	CCGG	5 cut(s)	39, 227, 351, 531, 665
BslFI	GGGAC	2 cut(s)	425, 579
BslI	CCNNNNNNNGG	6 cut(s)	38, 162, 163, 186, 187, 1311
BsmFI	GGGAC	2 cut(s)	425, 579
BsmI	GAATGC	2 cut(s)	31, 246
BsnI	GGCC	4 cut(s)	312, 354, 958, 1200
BsoBI	CYCGRG	2 cut(s)	53, 1043
Bsp1286I	GDGCHC	9 cut(s)	54, 169, 193, 338, 525, 624, 1050, 1416, 1609
Bsp1407I	TGTACA	1 cut(s)	1419
Bsp143I	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
Bsp1720I	GCTNAGC	1 cut(s)	93
Bsp19I	CCATGG	1 cut(s)	953
BspACI	CCGC	7 cut(s)	80, 233, 236, 588, 805, 1068, 1455
BspANI	GGCC	4 cut(s)	312, 354, 958, 1200
BspCNI	CTCAG	7 cut(s)	106, 112, 331, 879, 889, 1071, 1531
BspFNI	CGCG	1 cut(s)	1279
BspLI	GGNNCC	3 cut(s)	231, 311, 1011
BspMAI	CTGCAG	1 cut(s)	1036
BspPI	GGATC	3 cut(s)	370, 1357, 1574
BspT107I	GGYRCC	1 cut(s)	229
BsrDI	GCAATG	3 cut(s)	708, 1137, 1596
BsrFI	RCCGGY	2 cut(s)	226, 350
BsrGI	TGTACA	1 cut(s)	1419
BsrI	ACTGG	2 cut(s)	218, 306
BssAI	RCCGGY	2 cut(s)	226, 350
BssECI	CCNNGG	3 cut(s)	185, 953, 1052
BssMI	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
BssNI	GRCGYC	1 cut(s)	230
BssT1I	CCWWGG	2 cut(s)	185, 953
Bst4CI	ACNGT	4 cut(s)	570, 653, 761, 974
BstACI	GRCGYC	1 cut(s)	230
BstAUI	TGTACA	1 cut(s)	1419
BstC8I	GCNNGC	3 cut(s)	174, 367, 644
BstDEI	CTNAG	7 cut(s)	93, 99, 339, 866, 897, 1058, 1539
BstDSI	CCRYGG	2 cut(s)	953, 1052
BstF5I	GGATG	4 cut(s)	59, 110, 697, 1330
BstFNI	CGCG	1 cut(s)	1279
BstH2I	RGCGCY	2 cut(s)	179, 233
BstHHI	GCGC	5 cut(s)	178, 232, 530, 562, 1279
BstKTI	GATC	6 cut(s)	378, 477, 550, 658, 1352, 1569
BstMBI	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
BstMCI	CGRYCG	1 cut(s)	550
BstMWI	GCNNNNNNNGC	7 cut(s)	15, 73, 173, 202, 366, 590, 1054
BstSCI	CCNGG	1 cut(s)	37
BstSFI	CTRYAG	2 cut(s)	10, 1032
BstSLI	GKGCMC	4 cut(s)	169, 193, 338, 624
BstUI	CGCG	1 cut(s)	1279
BstV2I	GAAGAC	1 cut(s)	1271
BsuRI	GGCC	4 cut(s)	312, 354, 958, 1200
BtgI	CCRYGG	2 cut(s)	953, 1052
BtsCI	GGATG	4 cut(s)	59, 110, 697, 1330
BtsI	GCAGTG	1 cut(s)	781
BtsIMutI	CAGTG	5 cut(s)	336, 781, 874, 979, 1227
Cac8I	GCNNGC	3 cut(s)	174, 367, 644
CfoI	GCGC	5 cut(s)	178, 232, 530, 562, 1279
Cfr10I	RCCGGY	2 cut(s)	226, 350
Cfr13I	GGNCC	2 cut(s)	310, 1009
Csp6I	GTAC	8 cut(s)	280, 499, 757, 875, 1242, 1420, 1435, 1459
CspCI	CAANNNNNGTGG	2 cut(s)	450, 485
CviAII	CATG	4 cut(s)	637, 954, 1321, 1499
CviQI	GTAC	8 cut(s)	280, 499, 757, 875, 1242, 1420, 1435, 1459
DdeI	CTNAG	7 cut(s)	93, 99, 339, 866, 897, 1058, 1539
DinI	GGCGCC	1 cut(s)	231
DpnI	GATC	6 cut(s)	377, 476, 549, 657, 1351, 1568
DpnII	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
EaeI	YGGCCR	2 cut(s)	352, 956
EciI	GGCGGA	1 cut(s)	1083
Ecl136II	GAGCTC	1 cut(s)	523
Eco130I	CCWWGG	2 cut(s)	185, 953
Eco147I	AGGCCT	1 cut(s)	1200
Eco24I	GRGCYC	3 cut(s)	54, 525, 1609
Eco47I	GGWCC	1 cut(s)	1009
Eco53kI	GAGCTC	1 cut(s)	523
Eco57I	CTGAAG	4 cut(s)	728, 816, 1203, 1283
Eco88I	CYCGRG	2 cut(s)	53, 1043
EcoICRI	GAGCTC	1 cut(s)	523
EcoO109I	RGGNCCY	1 cut(s)	1009
EcoRI	GAATTC	1 cut(s)	1580
EcoT14I	CCWWGG	2 cut(s)	185, 953
EcoT22I	ATGCAT	1 cut(s)	1389
EcoT38I	GRGCYC	3 cut(s)	54, 525, 1609
EgeI	GGCGCC	1 cut(s)	231
EheI	GGCGCC	1 cut(s)	231
ErhI	CCWWGG	2 cut(s)	185, 953
FaeI	CATG	4 cut(s)	640, 957, 1324, 1502
FaqI	GGGAC	2 cut(s)	425, 579
FatI	CATG	4 cut(s)	636, 953, 1320, 1498
FauI	CCCGC	1 cut(s)	1448
FokI	GGATG	4 cut(s)	46, 97, 704, 1337
FriOI	GRGCYC	3 cut(s)	54, 525, 1609
FspBI	CTAG	2 cut(s)	684, 1110
GlaI	GCGC	5 cut(s)	177, 231, 529, 561, 1278
GsaI	CCCAGC	2 cut(s)	67, 160
GsuI	CTGGAG	1 cut(s)	201
HaeII	RGCGCY	2 cut(s)	179, 233
HaeIII	GGCC	4 cut(s)	312, 354, 958, 1200
HapII	CCGG	5 cut(s)	39, 227, 351, 531, 665
HhaI	GCGC	5 cut(s)	178, 232, 530, 562, 1279
Hin1I	GRCGYC	1 cut(s)	230
Hin1II	CATG	4 cut(s)	640, 957, 1324, 1502
Hin6I	GCGC	5 cut(s)	176, 230, 528, 560, 1277
HinP1I	GCGC	5 cut(s)	176, 230, 528, 560, 1277
HincII	GTYRAC	1 cut(s)	950
HindII	GTYRAC	1 cut(s)	950
HinfI	GANTC	4 cut(s)	430, 595, 846, 1174
HpaII	CCGG	5 cut(s)	39, 227, 351, 531, 665
HphI	GGTGA	3 cut(s)	526, 1207, 1520
Hpy166II	GTNNAC	6 cut(s)	336, 622, 757, 875, 950, 1215
Hpy188III	TCNNGA	4 cut(s)	379, 421, 1043, 1110
Hpy8I	GTNNAC	6 cut(s)	336, 622, 757, 875, 950, 1215
HpyAV	CCTTC	6 cut(s)	691, 703, 740, 920, 1153, 1486
HpyCH4III	ACNGT	4 cut(s)	570, 653, 761, 974
HpyF10VI	GCNNNNNNNGC	7 cut(s)	15, 73, 173, 202, 366, 590, 1054
HpyF3I	CTNAG	7 cut(s)	93, 99, 339, 866, 897, 1058, 1539
Hsp92I	GRCGYC	1 cut(s)	230
Hsp92II	CATG	4 cut(s)	640, 957, 1324, 1502
HspAI	GCGC	5 cut(s)	176, 230, 528, 560, 1277
KasI	GGCGCC	1 cut(s)	229
Kzo9I	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
LmnI	GCTCC	3 cut(s)	34, 520, 1495
LweI	GCATC	5 cut(s)	904, 987, 1085, 1315, 1537
MaeI	CTAG	2 cut(s)	684, 1110
MaeIII	GTNAC	3 cut(s)	602, 629, 1287
MalI	GATC	6 cut(s)	377, 476, 549, 657, 1351, 1568
MboI	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
MboII	GAAGA	4 cut(s)	1196, 1199, 1268, 1276
MfeI	CAATTG	1 cut(s)	1353
MhlI	GDGCHC	9 cut(s)	54, 169, 193, 338, 525, 624, 1050, 1416, 1609
MlsI	TGGCCA	1 cut(s)	958
MluNI	TGGCCA	1 cut(s)	958
Mly113I	GGCGCC	1 cut(s)	230
MlyI	GAGTC	1 cut(s)	855
Mox20I	TGGCCA	1 cut(s)	958
Mph1103I	ATGCAT	1 cut(s)	1389
MroXI	GAANNNNTTC	2 cut(s)	1027, 1121
MscI	TGGCCA	1 cut(s)	958
MseI	TTAA	4 cut(s)	273, 1194, 1203, 1630
MslI	CAYNNNNRTG	3 cut(s)	331, 641, 1220
Msp20I	TGGCCA	1 cut(s)	958
MspA1I	CMGCKG	2 cut(s)	590, 1455
MspI	CCGG	5 cut(s)	39, 227, 351, 531, 665
MspR9I	CCNGG	1 cut(s)	39
MunI	CAATTG	1 cut(s)	1353
Mva1269I	GAATGC	2 cut(s)	31, 246
MvnI	CGCG	1 cut(s)	1279
MwoI	GCNNNNNNNGC	7 cut(s)	15, 73, 173, 202, 366, 590, 1054
NarI	GGCGCC	1 cut(s)	230
NciI	CCSGG	1 cut(s)	39
NcoI	CCATGG	1 cut(s)	953
NdeII	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
NlaIII	CATG	4 cut(s)	640, 957, 1324, 1502
NlaIV	GGNNCC	3 cut(s)	231, 311, 1011
NmeAIII	GCCGAG	1 cut(s)	270
NmuCI	GTSAC	3 cut(s)	602, 629, 1287
NsiI	ATGCAT	1 cut(s)	1389
PaeR7I	CTCGAG	1 cut(s)	1043
PceI	AGGCCT	1 cut(s)	1200
PcsI	WCGNNNNNNNCGW	1 cut(s)	411
PctI	GAATGC	2 cut(s)	31, 246
PdmI	GAANNNNTTC	2 cut(s)	1027, 1121
PfeI	GAWTC	3 cut(s)	430, 595, 1174
Ple19I	CGATCG	1 cut(s)	550
PleI	GAGTC	1 cut(s)	854
PluTI	GGCGCC	1 cut(s)	233
PpsI	GAGTC	1 cut(s)	854
PpuMI	RGGWCCY	1 cut(s)	1009
Psp124BI	GAGCTC	1 cut(s)	525
Psp5II	RGGWCCY	1 cut(s)	1009
PspFI	CCCAGC	2 cut(s)	63, 156
PspN4I	GGNNCC	3 cut(s)	231, 311, 1011
PspPI	GGNCC	2 cut(s)	310, 1009
PspPPI	RGGWCCY	1 cut(s)	1009
PstI	CTGCAG	1 cut(s)	1036
PvuI	CGATCG	1 cut(s)	550
RsaI	GTAC	8 cut(s)	281, 500, 758, 876, 1243, 1421, 1436, 1460
RsaNI	GTAC	8 cut(s)	280, 499, 757, 875, 1242, 1420, 1435, 1459
RseI	CAYNNNNRTG	3 cut(s)	331, 641, 1220
SacI	GAGCTC	1 cut(s)	525
SaqAI	TTAA	4 cut(s)	273, 1194, 1203, 1630
Sau3AI	GATC	6 cut(s)	375, 474, 547, 655, 1349, 1566
Sau96I	GGNCC	2 cut(s)	310, 1009
ScaI	AGTACT	2 cut(s)	281, 1436
SchI	GAGTC	1 cut(s)	855
ScrFI	CCNGG	1 cut(s)	39
SduI	GDGCHC	9 cut(s)	54, 169, 193, 338, 525, 624, 1050, 1416, 1609
SfaNI	GCATC	5 cut(s)	904, 987, 1085, 1315, 1537
SfcI	CTRYAG	2 cut(s)	10, 1032
SfoI	GGCGCC	1 cut(s)	231
Sfr274I	CTCGAG	1 cut(s)	1043
SinI	GGWCC	1 cut(s)	1009
SlaI	CTCGAG	1 cut(s)	1043
SmiMI	CAYNNNNRTG	3 cut(s)	331, 641, 1220
SmlI	CTYRAG	3 cut(s)	659, 1043, 1142
SmoI	CTYRAG	3 cut(s)	659, 1043, 1142
SseBI	AGGCCT	1 cut(s)	1200
SsiI	CCGC	7 cut(s)	80, 233, 236, 588, 805, 1068, 1455
SspDI	GGCGCC	1 cut(s)	229
SspMI	CTAG	2 cut(s)	684, 1110
SstI	GAGCTC	1 cut(s)	525
StuI	AGGCCT	1 cut(s)	1200
StyD4I	CCNGG	1 cut(s)	37
StyI	CCWWGG	2 cut(s)	185, 953
TaaI	ACNGT	4 cut(s)	570, 653, 761, 974
TaqI	TCGA	6 cut(s)	374, 546, 550, 912, 1044, 1338
TatI	WGTACW	3 cut(s)	279, 1419, 1434
TauI	GCSGC	5 cut(s)	82, 235, 238, 590, 808
TfiI	GAWTC	3 cut(s)	430, 595, 1174
Tru1I	TTAA	4 cut(s)	273, 1194, 1203, 1630
Tru9I	TTAA	4 cut(s)	273, 1194, 1203, 1630
TscAI	CASTG	5 cut(s)	343, 781, 874, 979, 1234
TseFI	GTSAC	3 cut(s)	602, 629, 1287
Tsp45I	GTSAC	3 cut(s)	602, 629, 1287
TspDTI	ATGAA	6 cut(s)	625, 727, 1130, 1148, 1488, 1505
TspGWI	ACGGA	1 cut(s)	731
TspRI	CASTG	5 cut(s)	343, 781, 874, 979, 1234
VneI	GTGCAC	2 cut(s)	334, 620
VpaK11BI	GGWCC	1 cut(s)	1009
XapI	RAATTY	3 cut(s)	670, 1367, 1580
XbaI	TCTAGA	1 cut(s)	1109
XhoI	CTCGAG	1 cut(s)	1043
XmnI	GAANNNNTTC	2 cut(s)	1027, 1121
XspI	CTAG	2 cut(s)	684, 1110
ZrmI	AGTACT	2 cut(s)	281, 1436
Zsp2I	ATGCAT	1 cut(s)	1389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.