Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002696

Physical Location & Seq

Genomic Coordinates Reverse (-)

1924344 .. 1928670

Gene Structure 4327 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG027500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGGTTGCTGTGGCAGCAAGACCAAACCCAATCCAGGTCCTAATAATGGGTATGTCTCCTCATCCATACCCATCTCTCATCACCACCCACAACAGCAACCCTTGTCTGTCTCAACCCAGCAGCCTCACAAAGTTTCAGCTCCTCCAACCCATAACCCACCTCCACCACCCCAACCCCAATACCAACCACCACCACCAAAGCCACACCCACAGCCAGTGAAGCCAGCAGTGCCGCCAAACAGCATTCTTGAGAAGCCCTTTGATGACATTAAGCAGTACTACACTCTGGGGAAAGAACTGGGCAGAGGCCAATTTGGGATCACGTACTTGTGCACAGAGAAGTCTACGGGTGACACTTATGCCTGTAAGTCAATCTTGAAGCGCAAACTGGTGAGTAAAAGTGACAGGGATGATATCAAGAGGGAGATACAGATCATGCAGCACATGTCTGGGCAGCGCAACATTGTGGACATCAAGGGGGCTTATGAGGACAGGTACTCTGTGCATTTGGTGATGGAGCTCTGTGCCGGAGGGGAGCTTTTCGATAGGATCATAGCGCAGGGAGTGTATTCGGAGAGAGCTGCCGCCGCGATTGTGAGGGACATTGTGAATGTTGTGCACAGTTGTCATGTTATGGCTGTGATGCACCGTGATCTCAAGCCCGAGAATTTCTTGCTGTCTAGTAAGGGGGAAGGGGCAATGCTCAAGGTTACGGATTTCGGGCTGTCTGTTTTTATTGAGGAAGGGAAGGTATACCGAGATATAGTAGGCAGTGCTTACTATGTTGCTCCTGAAGTGTTACGGCGTAGTTATGGAAAGGAGATAGATATTTGGAGTGCTGGAGTAATTCTGTATATTCTACTTAGTGGAGTACCTCCATTTTGGGCTGAGACTGAGAGGGGCATCTTTAATGCAATATTGGAAGGTGTAGTTGACTTTGAAAGTCAACCATGGCCGTCAATATCAGACGGTGCCAAAGACCTAGTTAGAAAGATGCTGACACAGGACCCAAAAAAGAGAATTACTTCACTGAAAGTTCTTGAGCATCCCTGGCTCAGAGTAGGTGGAGAAGCATCAGACAAGCCAATAGATAGTGCAGTTCTCTCTAGAATGAAGCAGTTGAGAGCAATGAATAAGCTTAAGAAGCTTGCCTTGAAGGTAATAGCGGAAAATCTATCTGAAGAAGAAATTAAAGGTCTTAAGGCAATGTTCACCAGTATGGACACCGACAAAAGTGGTACAATAACCTACGAAGAACTGAAGACAGGATTAGCTCGTATTGGGTCACGGCTATCAGAAACCGAAGTCAGGCAACTTATGGATGCTGCTGACGTTGATGGAAATGGATCAATTGACTATATTGAATTTATATCTGCTACTATGCATAGACAAAGACTAGAGAGAGATGAACACCTGTACAAAGCATTTCAGTATTTTGACAAGGATAGCAGTGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009751 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0019899 GO:0019902 GO:0019903 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 Pkinase

Protein Analysis

485

Amino Acids

54.15

Weight (kDa)

8.05

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	94 - 352	2.2e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	95 - 345	8.5e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	399 - 460	3.3e-12	EF-hand domain pair
EF-hand_1	PF00036	400 - 426	2.5e-06	EF hand domain
EF-hand_8	PF13833	412 - 460	2.5e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61950 AT4G04710 AT4G04710 AT4G04710 AT4G04710 AT4G04720 AT4G04720 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G04740 AT4G21940 AT4G21940 AT4G21940
fragaria_vesca	FvH4_3g02130 FvH4_3g02130 FvH4_3g02130 FvH4_3g02130 FvH4_3g02130
malus_domestica	MD05G1345600.v1.1 MD10G1321000.v1.1
prunus_persica	Prupe.4G021300_v2.0.a1
pyrus_communis	pycom11g19140
rosa_chinensis	RchiOBHm_Chr5g0003201
rosa_laevigata	RLG00000031086
rosa_multiflora	Rmu_co8095158.1_g000001 Rmu_co8435131.1_g000001 Rmu_sc0000146.1_g000036
rosa_roxburghii	Rroxscaffold_1G00072810
rosa_rugosa	Rorug04G0401400
rosa_samantha	Rh5AG026300 Rh5BG027500 Rh5CG029100 Rh5DG025900
rosa_wichuraiana	Rw0G023540 Rw5G002430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	971
AccI	GTMKAC	2 cut(s)	344, 753
AccII	CGCG	1 cut(s)	590
AciI	CCGC	4 cut(s)	233, 585, 588, 1166
AclWI	GGATC	3 cut(s)	326, 557, 1354
AcoI	YGGCCR	1 cut(s)	953
AcsI	RAATTY	2 cut(s)	667, 1364
AcuI	CTGAAG	3 cut(s)	813, 1200, 1280
AfaI	GTAC	6 cut(s)	278, 326, 497, 873, 1240, 1418
AfiI	CCNNNNNNNGG	3 cut(s)	35, 47, 1308
AflII	CTTAAG	2 cut(s)	1139, 1199
AflIII	ACRYGT	1 cut(s)	444
AgsI	TTSAA	4 cut(s)	379, 941, 1156, 1364
AjnI	CCWGG	2 cut(s)	34, 1049
AluBI	AGCT	7 cut(s)	140, 520, 538, 581, 1138, 1147, 1274
AluI	AGCT	7 cut(s)	140, 520, 538, 581, 1138, 1147, 1274
Alw21I	GWGCWC	3 cut(s)	335, 522, 621
Alw26I	GTCTC	3 cut(s)	61, 115, 884
Alw44I	GTGCAC	2 cut(s)	331, 617
AlwI	GGATC	3 cut(s)	326, 557, 1354
Ama87I	CYCGRG	1 cut(s)	662
AoxI	GGCC	2 cut(s)	307, 953
ApaLI	GTGCAC	2 cut(s)	331, 617
ApeKI	GCWGC	6 cut(s)	15, 121, 439, 454, 581, 1325
ApoI	RAATTY	2 cut(s)	667, 1364
Asp700I	GAANNNNTTC	1 cut(s)	1024
AspLEI	GCGC	3 cut(s)	384, 459, 559
AspS9I	GGNCC	2 cut(s)	38, 1006
AsuHPI	GGTGA	5 cut(s)	74, 362, 403, 523, 1204
AvaI	CYCGRG	1 cut(s)	662
AvaII	GGWCC	2 cut(s)	38, 1006
BaeGI	GKGCMC	2 cut(s)	335, 621
BanI	GGYRCC	1 cut(s)	971
BanII	GRGCYC	1 cut(s)	522
BbsI	GAAGAC	1 cut(s)	1268
Bbv12I	GWGCWC	3 cut(s)	335, 522, 621
BbvI	GCAGC	6 cut(s)	27, 133, 451, 466, 568, 1312
BccI	CCATC	3 cut(s)	80, 508, 1331
BceAI	ACGGC	3 cut(s)	818, 940, 1304
BciT130I	CCWGG	2 cut(s)	36, 1051
BcoDI	GTCTC	3 cut(s)	61, 115, 884
BfaI	CTAG	4 cut(s)	681, 983, 1107, 1397
BfrI	CTTAAG	2 cut(s)	1139, 1199
BisI	GCNGC	9 cut(s)	16, 122, 233, 440, 455, 582, 585, 588, 1326
BlsI	GCNGC	9 cut(s)	17, 123, 234, 441, 456, 583, 586, 589, 1327
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	2 cut(s)	36, 1051
Bme18I	GGWCC	2 cut(s)	38, 1006
BmeT110I	CYCGRG	1 cut(s)	662
BmgT120I	GGNCC	2 cut(s)	38, 1006
BmiI	GGNNCC	2 cut(s)	973, 1008
BmrFI	CCNGG	2 cut(s)	36, 1051
BmrI	ACTGGG	1 cut(s)	308
BmsI	GCATC	6 cut(s)	633, 912, 984, 1054, 1082, 1312
BmuI	ACTGGG	1 cut(s)	308
BpiI	GAAGAC	1 cut(s)	1268
BpmI	CTGGAG	1 cut(s)	861
BpuEI	CTTGAG	4 cut(s)	269, 641, 689, 1061
BsaAI	YACGTR	1 cut(s)	324
BsaBI	GATNNNNATC	1 cut(s)	431
BsaJI	CCNNGG	2 cut(s)	950, 1049
Bsc4I	CCNNNNNNNGG	3 cut(s)	35, 47, 1308
Bse1I	ACTGG	4 cut(s)	215, 303, 393, 1215
Bse3DI	GCAATG	3 cut(s)	705, 1134, 1212
Bse8I	GATNNNNATC	1 cut(s)	431
BseBI	CCWGG	2 cut(s)	36, 1051
BseDI	CCNNGG	2 cut(s)	950, 1049
BseGI	GGATG	4 cut(s)	62, 415, 1045, 1327
BseJI	GATNNNNATC	1 cut(s)	431
BseLI	CCNNNNNNNGG	3 cut(s)	35, 47, 1308
BseMI	GCAATG	3 cut(s)	705, 1134, 1212
BseMII	CTCAG	3 cut(s)	879, 885, 1069
BseNI	ACTGG	4 cut(s)	215, 303, 393, 1215
BseRI	GAGGAG	2 cut(s)	49, 132
BseSI	GKGCMC	2 cut(s)	335, 621
BseXI	GCAGC	6 cut(s)	27, 133, 451, 466, 568, 1312
BseYI	CCCAGC	1 cut(s)	117
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	1 cut(s)	590
BshFI	GGCC	2 cut(s)	309, 955
BshNI	GGYRCC	1 cut(s)	971
BsiHKAI	GWGCWC	3 cut(s)	335, 522, 621
BsiHKCI	CYCGRG	1 cut(s)	662
BsiSI	CCGG	1 cut(s)	528
BslFI	GGGAC	1 cut(s)	614
BslI	CCNNNNNNNGG	3 cut(s)	35, 47, 1308
BsmAI	GTCTC	3 cut(s)	61, 115, 884
BsmFI	GGGAC	1 cut(s)	614
BsmI	GAATGC	1 cut(s)	243
BsnI	GGCC	2 cut(s)	309, 955
BsoBI	CYCGRG	1 cut(s)	662
Bsp1286I	GDGCHC	3 cut(s)	335, 522, 621
Bsp1407I	TGTACA	1 cut(s)	1416
Bsp143I	GATC	5 cut(s)	318, 432, 549, 652, 1346
Bsp19I	CCATGG	1 cut(s)	950
BspACI	CCGC	4 cut(s)	233, 585, 588, 1166
BspANI	GGCC	2 cut(s)	309, 955
BspCNI	CTCAG	3 cut(s)	880, 886, 1068
BspFNI	CGCG	1 cut(s)	590
BspLI	GGNNCC	2 cut(s)	973, 1008
BspPI	GGATC	3 cut(s)	326, 557, 1354
BspT107I	GGYRCC	1 cut(s)	971
BspTI	CTTAAG	2 cut(s)	1139, 1199
BsrDI	GCAATG	3 cut(s)	705, 1134, 1212
BsrGI	TGTACA	1 cut(s)	1416
BsrI	ACTGG	4 cut(s)	215, 303, 393, 1215
BssECI	CCNNGG	2 cut(s)	950, 1049
BssMI	GATC	5 cut(s)	318, 432, 549, 652, 1346
BssNAI	GTATAC	1 cut(s)	754
BssT1I	CCWWGG	1 cut(s)	950
Bst1107I	GTATAC	1 cut(s)	754
Bst2UI	CCWGG	2 cut(s)	36, 1051
Bst4CI	ACNGT	3 cut(s)	623, 650, 971
BstAFI	CTTAAG	2 cut(s)	1139, 1199
BstAUI	TGTACA	1 cut(s)	1416
BstBAI	YACGTR	1 cut(s)	324
BstC8I	GCNNGC	2 cut(s)	225, 1149
BstDEI	CTNAG	4 cut(s)	863, 888, 894, 1055
BstDSI	CCRYGG	1 cut(s)	950
BstF5I	GGATG	4 cut(s)	62, 415, 1045, 1327
BstFNI	CGCG	1 cut(s)	590
BstHHI	GCGC	3 cut(s)	384, 459, 559
BstKTI	GATC	5 cut(s)	321, 435, 552, 655, 1349
BstMAI	GTCTC	3 cut(s)	61, 115, 884
BstMBI	GATC	5 cut(s)	318, 432, 549, 652, 1346
BstMWI	GCNNNNNNNGC	6 cut(s)	15, 220, 229, 587, 1051, 1144
BstNI	CCWGG	2 cut(s)	36, 1051
BstNSI	RCATGY	1 cut(s)	448
BstSCI	CCNGG	2 cut(s)	34, 1049
BstSLI	GKGCMC	2 cut(s)	335, 621
BstUI	CGCG	1 cut(s)	590
BstV1I	GCAGC	6 cut(s)	27, 133, 451, 466, 568, 1312
BstV2I	GAAGAC	1 cut(s)	1268
BstZ17I	GTATAC	1 cut(s)	754
BsuRI	GGCC	2 cut(s)	309, 955
BtgI	CCRYGG	1 cut(s)	950
BtsCI	GGATG	4 cut(s)	62, 415, 1045, 1327
BtsI	GCAGTG	3 cut(s)	234, 778, 1456
BtsIMutI	CAGTG	5 cut(s)	222, 234, 778, 1028, 1456
Cac8I	GCNNGC	2 cut(s)	225, 1149
CfoI	GCGC	3 cut(s)	384, 459, 559
Cfr13I	GGNCC	2 cut(s)	38, 1006
Csp6I	GTAC	6 cut(s)	277, 325, 496, 872, 1239, 1417
CviAII	CATG	4 cut(s)	436, 445, 629, 951
CviQI	GTAC	6 cut(s)	277, 325, 496, 872, 1239, 1417
DdeI	CTNAG	4 cut(s)	863, 888, 894, 1055
DpnI	GATC	5 cut(s)	320, 434, 551, 654, 1348
DpnII	GATC	5 cut(s)	318, 432, 549, 652, 1346
EaeI	YGGCCR	1 cut(s)	953
Ecl136II	GAGCTC	1 cut(s)	520
Eco130I	CCWWGG	1 cut(s)	950
Eco24I	GRGCYC	1 cut(s)	522
Eco32I	GATATC	1 cut(s)	415
Eco47I	GGWCC	2 cut(s)	38, 1006
Eco53kI	GAGCTC	1 cut(s)	520
Eco57I	CTGAAG	3 cut(s)	813, 1200, 1280
Eco88I	CYCGRG	1 cut(s)	662
EcoICRI	GAGCTC	1 cut(s)	520
EcoO109I	RGGNCCY	2 cut(s)	38, 1006
EcoRII	CCWGG	2 cut(s)	34, 1049
EcoRV	GATATC	1 cut(s)	415
EcoT14I	CCWWGG	1 cut(s)	950
EcoT22I	ATGCAT	1 cut(s)	1386
EcoT38I	GRGCYC	1 cut(s)	522
ErhI	CCWWGG	1 cut(s)	950
FaeI	CATG	4 cut(s)	439, 448, 632, 954
FalI	AAGNNNNNCTT	4 cut(s)	359, 391, 1136, 1168
FaqI	GGGAC	1 cut(s)	614
FatI	CATG	4 cut(s)	435, 444, 628, 950
FblI	GTMKAC	2 cut(s)	344, 753
Fnu4HI	GCNGC	9 cut(s)	16, 122, 233, 440, 455, 582, 585, 588, 1326
FokI	GGATG	4 cut(s)	49, 422, 1032, 1334
FriOI	GRGCYC	1 cut(s)	522
Fsp4HI	GCNGC	9 cut(s)	16, 122, 233, 440, 455, 582, 585, 588, 1326
FspBI	CTAG	4 cut(s)	681, 983, 1107, 1397
GlaI	GCGC	3 cut(s)	383, 458, 558
GluI	GCNGC	9 cut(s)	16, 122, 233, 440, 455, 582, 585, 588, 1326
GsaI	CCCAGC	1 cut(s)	121
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	2 cut(s)	309, 955
HapII	CCGG	1 cut(s)	528
HhaI	GCGC	3 cut(s)	384, 459, 559
Hin1II	CATG	4 cut(s)	439, 448, 632, 954
Hin6I	GCGC	3 cut(s)	382, 457, 557
HinP1I	GCGC	3 cut(s)	382, 457, 557
HincII	GTYRAC	2 cut(s)	934, 947
HindII	GTYRAC	2 cut(s)	934, 947
HindIII	AAGCTT	2 cut(s)	1136, 1145
HpaII	CCGG	1 cut(s)	528
HphI	GGTGA	5 cut(s)	74, 362, 403, 523, 1204
Hpy166II	GTNNAC	8 cut(s)	333, 345, 469, 619, 754, 934, 947, 1212
Hpy188I	TCNGA	6 cut(s)	574, 967, 1058, 1078, 1180, 1297
Hpy188III	TCNNGA	6 cut(s)	248, 376, 418, 791, 1040, 1107
Hpy8I	GTNNAC	8 cut(s)	333, 345, 469, 619, 754, 934, 947, 1212
HpyAV	CCTTC	5 cut(s)	686, 737, 742, 917, 1150
HpyCH4III	ACNGT	3 cut(s)	623, 650, 971
HpyCH4IV	ACGT	2 cut(s)	323, 1332
HpyCH4V	TGCA	8 cut(s)	333, 439, 505, 619, 646, 914, 1097, 1384
HpyF10VI	GCNNNNNNNGC	6 cut(s)	15, 220, 229, 587, 1051, 1144
HpyF3I	CTNAG	4 cut(s)	863, 888, 894, 1055
HpySE526I	ACGT	2 cut(s)	323, 1332
Hsp92II	CATG	4 cut(s)	439, 448, 632, 954
HspAI	GCGC	3 cut(s)	382, 457, 557
Kzo9I	GATC	5 cut(s)	318, 432, 549, 652, 1346
LmnI	GCTCC	4 cut(s)	145, 517, 535, 793
Lsp1109I	GCAGC	6 cut(s)	27, 133, 451, 466, 568, 1312
LweI	GCATC	6 cut(s)	633, 912, 984, 1054, 1082, 1312
MaeI	CTAG	4 cut(s)	681, 983, 1107, 1397
MaeII	ACGT	2 cut(s)	323, 1332
MaeIII	GTNAC	5 cut(s)	350, 401, 709, 798, 1284
MalI	GATC	5 cut(s)	320, 434, 551, 654, 1348
MboI	GATC	5 cut(s)	318, 432, 549, 652, 1346
MboII	GAAGA	4 cut(s)	1193, 1196, 1265, 1273
MfeI	CAATTG	1 cut(s)	1350
MhlI	GDGCHC	3 cut(s)	335, 522, 621
MluCI	AATT	7 cut(s)	311, 667, 846, 1020, 1188, 1350, 1364
MmeI	TCCRAC	1 cut(s)	170
Mph1103I	ATGCAT	1 cut(s)	1386
MroXI	GAANNNNTTC	1 cut(s)	1024
MseI	TTAA	5 cut(s)	270, 909, 1140, 1191, 1200
MslI	CAYNNNNRTG	1 cut(s)	1217
MspCI	CTTAAG	2 cut(s)	1139, 1199
MspI	CCGG	1 cut(s)	528
MspR9I	CCNGG	2 cut(s)	36, 1051
MunI	CAATTG	1 cut(s)	1350
Mva1269I	GAATGC	1 cut(s)	243
MvaI	CCWGG	2 cut(s)	36, 1051
MvnI	CGCG	1 cut(s)	590
MwoI	GCNNNNNNNGC	6 cut(s)	15, 220, 229, 587, 1051, 1144
NcoI	CCATGG	1 cut(s)	950
NdeII	GATC	5 cut(s)	318, 432, 549, 652, 1346
NlaIII	CATG	4 cut(s)	439, 448, 632, 954
NlaIV	GGNNCC	2 cut(s)	973, 1008
NmuCI	GTSAC	3 cut(s)	350, 401, 1284
NsiI	ATGCAT	1 cut(s)	1386
NspI	RCATGY	1 cut(s)	448
PciI	ACATGT	1 cut(s)	444
PctI	GAATGC	1 cut(s)	243
PdmI	GAANNNNTTC	1 cut(s)	1024
PkrI	GCNGC	9 cut(s)	17, 123, 234, 441, 456, 583, 586, 589, 1327
Ppu21I	YACGTR	1 cut(s)	324
PpuMI	RGGWCCY	2 cut(s)	38, 1006
PscI	ACATGT	1 cut(s)	444
Psp124BI	GAGCTC	1 cut(s)	522
Psp5II	RGGWCCY	2 cut(s)	38, 1006
Psp6I	CCWGG	2 cut(s)	34, 1049
PspFI	CCCAGC	1 cut(s)	117
PspGI	CCWGG	2 cut(s)	34, 1049
PspN4I	GGNNCC	2 cut(s)	973, 1008
PspPI	GGNCC	2 cut(s)	38, 1006
PspPPI	RGGWCCY	2 cut(s)	38, 1006
RsaI	GTAC	6 cut(s)	278, 326, 497, 873, 1240, 1418
RsaNI	GTAC	6 cut(s)	277, 325, 496, 872, 1239, 1417
RseI	CAYNNNNRTG	1 cut(s)	1217
SacI	GAGCTC	1 cut(s)	522
SaqAI	TTAA	5 cut(s)	270, 909, 1140, 1191, 1200
SatI	GCNGC	9 cut(s)	16, 122, 233, 440, 455, 582, 585, 588, 1326
Sau3AI	GATC	5 cut(s)	318, 432, 549, 652, 1346
Sau96I	GGNCC	2 cut(s)	38, 1006
ScaI	AGTACT	1 cut(s)	278
ScrFI	CCNGG	2 cut(s)	36, 1051
SduI	GDGCHC	3 cut(s)	335, 522, 621
SfaNI	GCATC	6 cut(s)	633, 912, 984, 1054, 1082, 1312
SinI	GGWCC	2 cut(s)	38, 1006
SmiMI	CAYNNNNRTG	1 cut(s)	1217
SmlI	CTYRAG	6 cut(s)	248, 656, 704, 1040, 1139, 1199
SmoI	CTYRAG	6 cut(s)	248, 656, 704, 1040, 1139, 1199
Sse9I	AATT	7 cut(s)	311, 667, 846, 1020, 1188, 1350, 1364
SsiI	CCGC	4 cut(s)	233, 585, 588, 1166
SspI	AATATT	1 cut(s)	918
SspMI	CTAG	4 cut(s)	681, 983, 1107, 1397
SstI	GAGCTC	1 cut(s)	522
StyD4I	CCNGG	2 cut(s)	34, 1049
StyI	CCWWGG	1 cut(s)	950
TaaI	ACNGT	3 cut(s)	623, 650, 971
TaiI	ACGT	2 cut(s)	326, 1335
TaqI	TCGA	1 cut(s)	543
TasI	AATT	7 cut(s)	311, 667, 846, 1020, 1188, 1350, 1364
TatI	WGTACW	2 cut(s)	276, 1416
TauI	GCSGC	3 cut(s)	235, 587, 590
Tru1I	TTAA	5 cut(s)	270, 909, 1140, 1191, 1200
Tru9I	TTAA	5 cut(s)	270, 909, 1140, 1191, 1200
TscAI	CASTG	5 cut(s)	222, 234, 778, 1035, 1456
TseFI	GTSAC	3 cut(s)	350, 401, 1284
TseI	GCWGC	6 cut(s)	15, 121, 439, 454, 581, 1325
Tsp45I	GTSAC	3 cut(s)	350, 401, 1284
TspDTI	ATGAA	3 cut(s)	1127, 1145, 1422
TspGWI	ACGGA	1 cut(s)	728
TspRI	CASTG	5 cut(s)	222, 234, 778, 1035, 1456
Vha464I	CTTAAG	2 cut(s)	1139, 1199
VneI	GTGCAC	2 cut(s)	331, 617
VpaK11BI	GGWCC	2 cut(s)	38, 1006
XapI	RAATTY	2 cut(s)	667, 1364
XbaI	TCTAGA	1 cut(s)	1106
XceI	RCATGY	1 cut(s)	448
XmiI	GTMKAC	2 cut(s)	344, 753
XmnI	GAANNNNTTC	1 cut(s)	1024
XspI	CTAG	4 cut(s)	681, 983, 1107, 1397
ZrmI	AGTACT	1 cut(s)	278
Zsp2I	ATGCAT	1 cut(s)	1386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.