MD06G1017600.v1.1

ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005106
Physical Location & Seq
Reverse (-)
2159373 .. 2162193
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1017600.v1.1.491

Sequence Viewer

Length: 504 bp
ATGGGTACGCTCAGGCGGAGCTTGTTGCGGCGGCGGTCGTTTAGGTCCGGTGTGGATATGGATGATAGAGGATGGACCATGCTGCACATTGGTTGTCGAAAGGGTGATCTTGGACAGGTGAGGCAGCTTCTTGACGAGGGAATGGATGTGAATGCTGCTGCTTGGGGTCACAAATCAAAAGGGGTTACCCCTCTCCACCTAGCTGCTGAGGGTGGCCATTTAGATGTTATGGATGAATTACTTGAGCGTGGAGCTAACATTGATGCCAGAACTAAGGGTGCTTGTGGCTGGACTCCGCTTCACAGTGCAGCCAAAGAGAGGAGGAGGGAAGCAGTGAAATTTCTGGTAGAAAATGGAGCATTCTTGCCAGATGACATGTATGATTGTAGGTTTAACCCTCCACTCCATTACTGCCCTGGTCTTGAGTGGGCTTATGAGGAGATGAAGCGTCTTCAGCTAGAAAGTGCATCGTCAGGGGAGAGCTCGTGCAGCTCTGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0001932 GO:0001933 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0003824 GO:0004842 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008037 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009553 GO:0009566 GO:0009567 GO:0009628 GO:0009639 GO:0009642 GO:0009644 GO:0009653 GO:0009743 GO:0009744 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009962 GO:0009963 GO:0009986 GO:0009987 GO:0009988 GO:0010033 GO:0010035 GO:0010038 GO:0010218 GO:0010256 GO:0010313 GO:0010468 GO:0010563 GO:0010604 GO:0010605 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0016567 GO:0016740 GO:0019220 GO:0019222 GO:0019226 GO:0019228 GO:0019538 GO:0019787 GO:0019899 GO:0019953 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022412 GO:0022414 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031537 GO:0031539 GO:0031540 GO:0031542 GO:0031594 GO:0031625 GO:0031674 GO:0032026 GO:0032268 GO:0032269 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0032991 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034285 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036211 GO:0036477 GO:0042221 GO:0042325 GO:0042326 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043170 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0043412 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044291 GO:0044304 GO:0044325 GO:0044389 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0044703 GO:0045026 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0045936 GO:0046907 GO:0048193 GO:0048229 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051171 GO:0051172 GO:0051174 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051246 GO:0051248 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051704 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061025 GO:0061564 GO:0061640 GO:0061936 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070647 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071704 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0080090 GO:0080173 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1901564 GO:1901700 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1902494 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990234 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

168

Amino Acids

18.65

Weight (kDa)

6.3

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 17 - 90 4.4e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 60 - 120 3.5e-14 Ankyrin repeats (3 copies)
Ank_5 PF13857 60 - 92 9.6e-06 Ankyrin repeats (many copies)
Ank PF00023 61 - 92 1.2e-09 Ankyrin repeat
Ank_3 PF13606 61 - 89 2.6e-06 Ankyrin repeat
Ank_4 PF13637 65 - 116 1.1e-11 Ankyrin repeats (many copies)
Ank_2 PF12796 81 - 137 4e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 16, 28, 31, 34, 296
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 437
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 375
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 8 cut(s) 21, 127, 203, 254, 457, 483, 492, 501
AluI AGCT 8 cut(s) 21, 127, 203, 254, 457, 483, 492, 501
Alw21I GWGCWC 1 cut(s) 485
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 7 cut(s) 82, 124, 155, 158, 203, 308, 489
ApoI RAATTY 1 cut(s) 338
AspS9I GGNCC 2 cut(s) 45, 75
AsuHPI GGTGA 2 cut(s) 116, 130
AvaII GGWCC 2 cut(s) 45, 75
BalI TGGCCA 1 cut(s) 216
BanII GRGCYC 1 cut(s) 485
BauI CACGAG 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 6 cut(s) 69, 136, 142, 145, 190, 320
BccI CCATC 1 cut(s) 66
BciT130I CCWGG 1 cut(s) 417
BfaI CTAG 2 cut(s) 200, 458
BisI GCNGC 9 cut(s) 29, 32, 83, 125, 156, 159, 204, 309, 490
BlsI GCNGC 9 cut(s) 30, 33, 84, 126, 157, 160, 205, 310, 491
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 2 cut(s) 45, 75
BmgT120I GGNCC 2 cut(s) 45, 75
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 253, 476
BpiI GAAGAC 1 cut(s) 443
Bpu10I CCTNAGC 2 cut(s) 11, 207
BpuEI CTTGAG 2 cut(s) 263, 443
BsaJI CCNNGG 1 cut(s) 415
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 415
BseGI GGATG 4 cut(s) 67, 77, 151, 238
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 25, 198
BseRI GAGGAG 3 cut(s) 334, 337, 452
BseXI GCAGC 6 cut(s) 69, 136, 142, 145, 190, 320
BsgI GTGCAG 2 cut(s) 68, 327
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 216
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 1 cut(s) 485
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 318
BsmI GAATGC 2 cut(s) 157, 359
BsnI GGCC 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 5 cut(s) 16, 28, 31, 34, 296
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 2 cut(s) 24, 199
BssECI CCNNGG 1 cut(s) 415
BssMI GATC 1 cut(s) 106
BssSI CACGAG 1 cut(s) 484
Bst2BI CACGAG 1 cut(s) 484
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 1 cut(s) 305
BstDEI CTNAG 3 cut(s) 11, 207, 273
BstEII GGTNACC 1 cut(s) 184
BstF5I GGATG 4 cut(s) 67, 77, 151, 238
BstKTI GATC 1 cut(s) 109
BstMBI GATC 1 cut(s) 106
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 3 cut(s) 454, 489, 498
BstNI CCWGG 1 cut(s) 417
BstNSI RCATGY 1 cut(s) 379
BstPI GGTNACC 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 415
BstV1I GCAGC 6 cut(s) 69, 136, 142, 145, 190, 320
BstV2I GAAGAC 1 cut(s) 443
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 4 cut(s) 67, 77, 151, 238
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 310, 339
Cfr13I GGNCC 2 cut(s) 45, 75
CseI GACGC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 79, 376
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 3 cut(s) 11, 207, 273
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 214
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 483
Eco24I GRGCYC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 45, 75
Eco53kI GAGCTC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 184
EcoICRI GAGCTC 1 cut(s) 483
EcoO65I GGTNACC 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 485
FaeI CATG 2 cut(s) 82, 379
FaiI YATR 6 cut(s) 59, 80, 230, 377, 381, 435
FatI CATG 2 cut(s) 78, 375
Fnu4HI GCNGC 9 cut(s) 29, 32, 83, 125, 156, 159, 204, 309, 490
FokI GGATG 4 cut(s) 74, 84, 158, 245
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 9 cut(s) 29, 32, 83, 125, 156, 159, 204, 309, 490
FspBI CTAG 2 cut(s) 200, 458
GluI GCNGC 9 cut(s) 29, 32, 83, 125, 156, 159, 204, 309, 490
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 437
Hin1II CATG 2 cut(s) 82, 379
HinfI GANTC 1 cut(s) 292
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 116, 130
Hpy188I TCNGA 1 cut(s) 496
Hpy188III TCNNGA 2 cut(s) 131, 422
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4V TGCA 4 cut(s) 85, 308, 467, 489
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 489, 498
HpyF3I CTNAG 3 cut(s) 11, 207, 273
Hsp92II CATG 2 cut(s) 82, 379
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 3 cut(s) 18, 251, 356
LpnPI CCDG 9 cut(s) 61, 101, 274, 280, 329, 381, 402, 429, 459
Lsp1109I GCAGC 6 cut(s) 69, 136, 142, 145, 190, 320
LweI GCATC 2 cut(s) 253, 476
MaeI CTAG 2 cut(s) 200, 458
MaeIII GTNAC 2 cut(s) 167, 184
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 1 cut(s) 443
MhlI GDGCHC 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 216
MluCI AATT 2 cut(s) 236, 338
MluNI TGGCCA 1 cut(s) 216
MlyI GAGTC 1 cut(s) 286
Mox20I TGGCCA 1 cut(s) 216
MscI TGGCCA 1 cut(s) 216
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 222
Msp20I TGGCCA 1 cut(s) 216
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 417
Mva1269I GAATGC 2 cut(s) 157, 359
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 454, 489, 498
NdeII GATC 1 cut(s) 106
NlaIII CATG 2 cut(s) 82, 379
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 379
PciI ACATGT 1 cut(s) 375
PctI GAATGC 2 cut(s) 157, 359
PkrI GCNGC 9 cut(s) 30, 33, 84, 126, 157, 160, 205, 310, 491
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 415
PspEI GGTNACC 1 cut(s) 184
PspGI CCWGG 1 cut(s) 415
PspPI GGNCC 2 cut(s) 45, 75
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 485
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 9 cut(s) 29, 32, 83, 125, 156, 159, 204, 309, 490
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 2 cut(s) 45, 75
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 2 cut(s) 253, 476
SinI GGWCC 2 cut(s) 45, 75
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 2 cut(s) 242, 422
SmoI CTYRAG 2 cut(s) 242, 422
Sse9I AATT 2 cut(s) 236, 338
SsiI CCGC 5 cut(s) 16, 28, 31, 34, 296
SspMI CTAG 2 cut(s) 200, 458
SstI GAGCTC 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 415
TaaI ACNGT 1 cut(s) 305
TaqI TCGA 1 cut(s) 97
TasI AATT 2 cut(s) 236, 338
TauI GCSGC 2 cut(s) 31, 34
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 2 cut(s) 310, 339
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 7 cut(s) 82, 124, 155, 158, 203, 308, 489
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 249, 458
TspRI CASTG 2 cut(s) 310, 339
VpaK11BI GGWCC 2 cut(s) 45, 75
XapI RAATTY 1 cut(s) 338
XceI RCATGY 1 cut(s) 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XspI CTAG 2 cut(s) 200, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.