Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Reverse (-)

34256443 .. 34262258

Gene Structure 5816 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1209100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGCGAGGTGGGGGTAGAAGAAAAAAGCAGCATTTTGGTAGAATCCATGATTTTTCATGTGGGAAAGCTTCCTTCAATGGCGAGCATTCACGGATTGGCGGCCCTGGTTTCTCGAGGGTAGTGTACTGTAATGACCCTGAATGCTTGGAGGCCACACTCCACAGCTACCAGGGCAATTATGTCAAAACGACTAAGTATACACTTGCTACATTCTTGCCCAAGGCAGTGTTTGAGCAGTTCAGAAGGGTTGCCAATTTATACTTCCTCATTTGTGCAATTCTGTCTTTCACGCCGCTTTCGCCTTACTCCGCCGTCAGCAACGTCGTCCCTCTCGTCGTCGTTATCGGAGTTACGATGGGGAAGGAGGCTGTTGAAGATTGGAGGAGGAAGAAACAGGACATTGAAATGAACAACAGAAAAGTTAAGGTGCATCATGGTGAGGGAAATTTTGAGCATACCAAATGGAGGGATTTGAAAGTTGGGGATATAGTGAAGGTCGAAAAGGACGAGTTTTTTCCTGCTGATCTTATCTTACTTTCATCAAGCTATGACGAAGCGCTTTGCTATGTTGAGACCACCAATCTTGATGGGGAAACTAATTTGAAACTAAAACAAGCACTGGAAGAAACTTCTAATCTGCATGAAGACTCAAGCTTTGAGAATTTTAAGGCGGTAATCAGATGTGAAGACCCAAATGCAAATTTGTACTCATTTGTTGGCACTATGGAGATTGAGGATCAACAGTACCCTCTTACACCACAGCAGCTTCTGCTTCGAGACTCAAAGCTGCGAAACACGGATTTTGTATATGGGGTGGTAATCTTCACAGGTCACGATACAAAAGTTATGCAGAATTCAACAGATCCTCCTTCTAAGAGAAGCAAAGTTGAGAAGCGGATGGATAAGATTATCTACTTCTTGTTTTTCCTCTTGGTTGTGATGTCTTTAGTTGGGGCTATTGTCTTCGGGGTTAGTACTAGTAAAGACCTGGATGATGGGAAAATGATCAGATGGTACCTCAGACCAGATGATACCACAGTTTACTATGATCCAACGAGGGCACCACTTGCAGCAGTTTTGCAATTTTTAACTGCCATTATGCTGTATAGTTATTTGATCCCCATTTCCTTGTATGTGTCAATAGAAATTGTCAAAGTTCTTCAGTGCACTTTTATAAACCATGATGTGCACATGTATTATGAGGAAACTGACAAGCCAGCCCGAGCGCGTACTTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATACTTTCAGATAAAACTGGAACATTAACTTGCAACTCGATGGAATTTATCAAGTGTTCTATTGCTGGCACTGCATTTGGGCGCGGAGTTACAGAAGTTGAGAGAGCCTTAGCCAGGAGAAAAGGTGAGGAATTGACAGAAGAAGAGATCCATGCTGAAGAAGAACTGAATGAAGCCAAGTCGATGATAAAAGGCTTTAATTTTATGGATGAAAGGATCATGAACGGAAAATGGGTTAATGAGCCTCACGCAGATGTAATCCAGAAGTTTCTACAGTTACTAGCAATCTGTCATACCGCAATACCTGAAGTTGATGAAGAAACTGGAAGAGTATCATATGAAGCTGAATCACCAGATGAGGCAGCTTTCGTGATTGCAGCAAGAGAGTTTGGGTTTGAATTTTACGAAAGGACTCAAGGAAGCATCTCACTGCATGAATTGGATCCCATTTATGGAAGAAAAATTGAAAGAAAATATAAACTTCTGAACATATTGGAGTTTAGTAGCTCAAGAAAGAGAATGTCCGTGATCATAAGAAACGAAGAGGGAAAGATACTACTCCTATGCAAAGGCGCTGACAGTGTCATGTTCGAAAGACTTGCAAAGAACGGAAGGGAGTTTGAAGAGAAGACCAGGGAACACATTAACAAGTATGCTGACGCTGGTTTGAGGACTTTGGTACTTGCATATCGCGAACTTGACGAAGAAGAATATGATGAGTTCAATAAAGAATTCACTGAGGCCAAAACCTTGGTGAGTTCAGATCGGGAGGAAATTGTTGAGGCAGTTTCAGAGAAGATTGAGAGGGACTTGATTCTTCTCGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCCGAATGCATTGACAAGCTTGCTCAGGCTGGAATTAAAATATGGGTTCTGACTGGAGATAAAATGGAAACAGCGATTAATATTGGATATGCGTGCAGTTTGCTCAGACAAGGAATGAAACAGATAGTAATAAGCTCCGAGACTGCAGAAGCTAAAGCATTAGAGAAGGTGGAGGATAAGACAGCAGTTGCCAAGGCATTGAAGGAAAGTGTAGTCCATCAAATTAGTCAGGGAAAGGCGTTGCTTGCTGCACCAGAAGAGAACTCTCAGGCACTGGCTTTGATCATTGATGGGAATTCGCTTGCTTATGCCTTAGAGAACGATGTCAAGGATCTCTTTCTAGAGCTTGCCATCGGCTGCGCATCTGTTATATGCTGTCGTTCGTCTCCCAAACAGAAAGCACTTGTTGCAAGACTGGTAAAAGATAAAACTGGCAACACAACTCTGGCCGTTGGTGATGGGGCAAATGATGTTGGAATGCTTCAAGAAGCAGATATCGGAGTTGGTATCAGCGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGCGACATTGCAATTGCACAGTTCCGATTTTTGGAGCGCCTACTACTTGTACATGGCCATTGGTGTTACAGAAGAATCTCATCAATGGTCTGCTATTTCTTCTACAAGAACATTGCTTTTGGCTTCACTATTTTCTTCTATCAGATATATGCTTCATTCTCGGGCCAAACTGCGTACAACGATTGGTATCTGTCACTATACAATGTCTTCTTCACGTCACTTCCGGTGGTTGCCTTGGGAGTTTTTGACCAAGATGTCTCGGCCAAGTTCTGTCTCAAGTTCCCGCTATTGTACCAAGAAGGTGCGCAAAACGTCTTATTTAGCTGGCTCCGGATACTTGGATGGGCAATGAACGGGGTGGTGACTGCTACCCTAGTCTTCTTCTTCTGCGTTGTGGCAATGGGCAGTCAGGCCTTTCGTAAAGGCGGGCAAGTTGTTGGCTTGGAAATCTTTGGTGCCACGATGTACTCATGTGTGGTATGGGTCGTCAACTGTCAAATGGCGCTGTCCATCAACTACTTCACTTATATACAACATCTCTTCATCTGGGGTGGCATCATATTCTGGTATATATTCCAGTTGGCATATGGAGCCATTGACCCCGACATATCAACCACCGCCTATAAGGTGTTCATTGAAGCCTGTGCACCGGCTCCGTTTTATTGGCTTCTCACACTGTTTGTTTTGGTCACTTCCCTCCTGCCATACTTCACTTACGCAGCCATCCAAATGCGGTTTTTCCCCATGTATCATCAGATGATACAGTGGATAAGAACAGACGGGCAATCAGATGACCCCGAGTTCTGTCAAATGGTGCGGCAGAGATCCATAAGAAGCACAACAGTAGGTTACACAGCCCGCATCGAAGCAACATCTAAACGCTTTGTAGAGAAGCCCGAAGGTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1194

Amino Acids

135.05

Weight (kDa)

5.54

Isoelectric Point (pI)

38.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 106	7.1e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	511 - 707	2e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	889 - 1139	1.6e-86	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1175
AasI	GACNNNNNNGTC	1 cut(s)	2927
Acc16I	TGCGCA	2 cut(s)	2491, 2979
Acc65I	GGTACC	1 cut(s)	1014
AccB1I	GGYRCC	3 cut(s)	1014, 1060, 3128
AccI	GTMKAC	1 cut(s)	197
AccII	CGCG	3 cut(s)	1228, 1353, 1962
AccIII	TCCGGA	1 cut(s)	3003
AcoI	YGGCCR	4 cut(s)	1255, 2577, 2728, 2934
AcsI	RAATTY	8 cut(s)	445, 661, 700, 853, 1313, 1667, 2000, 2425
AcuI	CTGAAG	3 cut(s)	1145, 1446, 1596
AfeI	AGCGCT	1 cut(s)	558
AfiI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1721, 2704
AflIII	ACRYGT	1 cut(s)	1191
AhlI	ACTAGT	1 cut(s)	977
AjiI	CACGTC	1 cut(s)	2889
AjnI	CCWGG	5 cut(s)	103, 168, 987, 1382, 1901
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
Alw21I	GWGCWC	3 cut(s)	1169, 1191, 3322
Alw26I	GTCTC	6 cut(s)	566, 771, 2264, 2520, 2935, 2951
Alw44I	GTGCAC	3 cut(s)	1165, 1187, 3318
AlwNI	CAGNNNCTG	2 cut(s)	769, 2404
Ama87I	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
Aor13HI	TCCGGA	1 cut(s)	3003
Aor51HI	AGCGCT	1 cut(s)	558
AoxI	GGCC	9 cut(s)	100, 150, 1255, 2010, 2577, 2728, 2836, 2934, 3084
ApaLI	GTGCAC	3 cut(s)	1165, 1187, 3318
ApeKI	GCWGC	9 cut(s)	28, 763, 787, 1070, 1631, 1646, 2378, 2487, 3392
ApoI	RAATTY	8 cut(s)	445, 661, 700, 853, 1313, 1667, 2000, 2425
ArsI	GACNNNNNNTTYG	2 cut(s)	638, 670
AseI	ATTAAT	1 cut(s)	2208
Asp718I	GGTACC	1 cut(s)	1014
AspLEI	GCGC	8 cut(s)	559, 1228, 1353, 1844, 2492, 2712, 2980, 3178
AspS9I	GGNCC	2 cut(s)	101, 2836
AsuHPI	GGTGA	6 cut(s)	449, 1406, 1611, 2035, 2597, 3046
AsuII	TTCGAA	1 cut(s)	1860
AvaI	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
BaeGI	GKGCMC	4 cut(s)	1063, 1169, 1191, 3322
BaeI	ACNNNNGTAYC	4 cut(s)	828, 861, 1023, 1056
BalI	TGGCCA	2 cut(s)	1257, 2730
BamHI	GGATCC	1 cut(s)	1711
BanI	GGYRCC	3 cut(s)	1014, 1060, 3128
BbsI	GAAGAC	7 cut(s)	651, 693, 955, 1904, 2115, 2872, 3043
Bbv12I	GWGCWC	3 cut(s)	1169, 1191, 3322
BbvI	GCAGC	9 cut(s)	40, 774, 775, 1082, 1643, 1658, 2365, 2474, 3404
BceAI	ACGGC	2 cut(s)	296, 2564
BcgI	CGANNNNNNTGC	2 cut(s)	1850, 1884
BciT130I	CCWGG	5 cut(s)	105, 170, 989, 1384, 1903
BciVI	GTATCC	1 cut(s)	3000
BclI	TGATCA	3 cut(s)	1005, 1797, 2412
BcoDI	GTCTC	6 cut(s)	566, 771, 2264, 2520, 2935, 2951
BcuI	ACTAGT	1 cut(s)	977
BfaI	CTAG	4 cut(s)	978, 1550, 2471, 3047
BfmI	CTRYAG	3 cut(s)	1541, 2100, 2274
BfoI	RGCGCY	4 cut(s)	560, 1845, 2713, 3179
BfuI	GTATCC	1 cut(s)	3000
BmcAI	AGTACT	1 cut(s)	976
Bme1390I	CCNGG	5 cut(s)	105, 170, 989, 1384, 1903
BmeT110I	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
BmgBI	CACGTC	1 cut(s)	2889
BmgT120I	GGNCC	2 cut(s)	101, 2836
BmiI	GGNNCC	8 cut(s)	1016, 1062, 1713, 2130, 3002, 3130, 3265, 3327
BmrFI	CCNGG	5 cut(s)	105, 170, 989, 1384, 1903
BmsI	GCATC	5 cut(s)	439, 1701, 2501, 3237, 3543
BpiI	GAAGAC	7 cut(s)	651, 693, 955, 1904, 2115, 2872, 3043
BpmI	CTGGAG	1 cut(s)	2205
Bpu10I	CCTNAGC	2 cut(s)	1378, 2154
Bpu14I	TTCGAA	1 cut(s)	1860
BpuEI	CTTGAG	4 cut(s)	634, 1668, 1762, 2933
BsaBI	GATNNNNATC	1 cut(s)	2859
BsaI	GGTCTC	1 cut(s)	566
BsaJI	CCNNGG	7 cut(s)	103, 169, 219, 1902, 2019, 2322, 2907
BsaWI	WCCGGW	2 cut(s)	2896, 3003
BsaXI	ACNNNNNCTCC	4 cut(s)	850, 880, 1378, 1408
Bsc4I	CCNNNNNNNGG	4 cut(s)	465, 1383, 1721, 2704
Bse118I	RCCGGY	1 cut(s)	3322
Bse1I	ACTGG	8 cut(s)	624, 1291, 1597, 2188, 2409, 2550, 2566, 3250
Bse3DI	GCAATG	4 cut(s)	2679, 2784, 3027, 3078
Bse8I	GATNNNNATC	1 cut(s)	2859
BseAI	TCCGGA	1 cut(s)	3003
BseBI	CCWGG	5 cut(s)	105, 170, 989, 1384, 1903
BseDI	CCNNGG	7 cut(s)	103, 169, 219, 1902, 2019, 2322, 2907
BseGI	GGATG	5 cut(s)	903, 997, 1483, 3020, 3396
BseJI	GATNNNNATC	1 cut(s)	2859
BseLI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1721, 2704
BseMI	GCAATG	4 cut(s)	2679, 2784, 3027, 3078
BseMII	CTCAG	5 cut(s)	1033, 1998, 2168, 2248, 2411
BseNI	ACTGG	8 cut(s)	624, 1291, 1597, 2188, 2409, 2550, 2566, 3250
BseRI	GAGGAG	1 cut(s)	397
BseSI	GKGCMC	4 cut(s)	1063, 1169, 1191, 3322
BseXI	GCAGC	9 cut(s)	40, 774, 775, 1082, 1643, 1658, 2365, 2474, 3404
BsgI	GTGCAG	2 cut(s)	2245, 2364
Bsh1236I	CGCG	3 cut(s)	1228, 1353, 1962
BshFI	GGCC	9 cut(s)	102, 152, 1257, 2012, 2579, 2730, 2838, 2936, 3086
BshNI	GGYRCC	3 cut(s)	1014, 1060, 3128
BsiHKAI	GWGCWC	3 cut(s)	1169, 1191, 3322
BsiHKCI	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
BsiSI	CCGG	3 cut(s)	2897, 3004, 3323
BslFI	GGGAC	2 cut(s)	311, 2090
BslI	CCNNNNNNNGG	4 cut(s)	465, 1383, 1721, 2704
BsmAI	GTCTC	6 cut(s)	566, 771, 2264, 2520, 2935, 2951
BsmBI	CGTCTC	1 cut(s)	2520
BsmFI	GGGAC	2 cut(s)	311, 2090
BsmI	GAATGC	5 cut(s)	85, 146, 2141, 2613, 2661
BsnI	GGCC	9 cut(s)	102, 152, 1257, 2012, 2579, 2730, 2838, 2936, 3086
Bso31I	GGTCTC	1 cut(s)	566
BsoBI	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
Bsp119I	TTCGAA	1 cut(s)	1860
Bsp1286I	GDGCHC	4 cut(s)	1063, 1169, 1191, 3322
Bsp13I	TCCGGA	1 cut(s)	3003
Bsp1407I	TGTACA	1 cut(s)	2722
Bsp68I	TCGCGA	1 cut(s)	1962
BspANI	GGCC	9 cut(s)	102, 152, 1257, 2012, 2579, 2730, 2838, 2936, 3086
BspCNI	CTCAG	5 cut(s)	1032, 1999, 2167, 2247, 2410
BspEI	TCCGGA	1 cut(s)	3003
BspFNI	CGCG	3 cut(s)	1228, 1353, 1962
BspHI	TCATGA	1 cut(s)	1488
BspLI	GGNNCC	8 cut(s)	1016, 1062, 1713, 2130, 3002, 3130, 3265, 3327
BspMAI	CTGCAG	2 cut(s)	2104, 2278
BspT104I	TTCGAA	1 cut(s)	1860
BspT107I	GGYRCC	3 cut(s)	1014, 1060, 3128
BspTNI	GGTCTC	1 cut(s)	566
BsrDI	GCAATG	4 cut(s)	2679, 2784, 3027, 3078
BsrFI	RCCGGY	1 cut(s)	3322
BsrGI	TGTACA	1 cut(s)	2722
BsrI	ACTGG	8 cut(s)	624, 1291, 1597, 2188, 2409, 2550, 2566, 3250
BssAI	RCCGGY	1 cut(s)	3322
BssECI	CCNNGG	7 cut(s)	103, 169, 219, 1902, 2019, 2322, 2907
BssNAI	GTATAC	1 cut(s)	198
BssT1I	CCWWGG	4 cut(s)	219, 2019, 2322, 2907
Bst1107I	GTATAC	1 cut(s)	198
Bst2UI	CCWGG	5 cut(s)	105, 170, 989, 1384, 1903
Bst6I	CTCTTC	6 cut(s)	1407, 1591, 1806, 1887, 2382, 3218
BstAPI	GCANNNNNTGC	3 cut(s)	769, 1067, 2537
BstAUI	TGTACA	1 cut(s)	2722
BstBI	TTCGAA	1 cut(s)	1860
BstDEI	CTNAG	9 cut(s)	192, 873, 1019, 1378, 2007, 2154, 2234, 2397, 2443
BstENI	CCTNNNNNAGG	1 cut(s)	1381
BstF5I	GGATG	5 cut(s)	903, 997, 1483, 3020, 3396
BstFNI	CGCG	3 cut(s)	1228, 1353, 1962
BstH2I	RGCGCY	4 cut(s)	560, 1845, 2713, 3179
BstHHI	GCGC	8 cut(s)	559, 1228, 1353, 1844, 2492, 2712, 2980, 3178
BstMAI	GTCTC	6 cut(s)	566, 771, 2264, 2520, 2935, 2951
BstMWI	GCNNNNNNNGC	8 cut(s)	171, 298, 769, 1067, 1340, 2375, 2537, 3263
BstNI	CCWGG	5 cut(s)	105, 170, 989, 1384, 1903
BstNSI	RCATGY	1 cut(s)	1195
BstSCI	CCNGG	5 cut(s)	103, 168, 987, 1382, 1901
BstSFI	CTRYAG	3 cut(s)	1541, 2100, 2274
BstSLI	GKGCMC	4 cut(s)	1063, 1169, 1191, 3322
BstUI	CGCG	3 cut(s)	1228, 1353, 1962
BstV1I	GCAGC	9 cut(s)	40, 774, 775, 1082, 1643, 1658, 2365, 2474, 3404
BstV2I	GAAGAC	7 cut(s)	651, 693, 955, 1904, 2115, 2872, 3043
BstX2I	RGATCY	5 cut(s)	862, 1416, 1711, 2461, 3497
BstXI	CCANNNNNNTGG	1 cut(s)	2020
BstYI	RGATCY	5 cut(s)	862, 1416, 1711, 2461, 3497
BstZ17I	GTATAC	1 cut(s)	198
BsuI	GTATCC	1 cut(s)	3000
BsuRI	GGCC	9 cut(s)	102, 152, 1257, 2012, 2579, 2730, 2838, 2936, 3086
BtrI	CACGTC	1 cut(s)	2889
BtsCI	GGATG	5 cut(s)	903, 997, 1483, 3020, 3396
BtsI	GCAGTG	3 cut(s)	231, 1338, 1697
BtuMI	TCGCGA	1 cut(s)	1962
CaiI	CAGNNNCTG	2 cut(s)	769, 2404
CciI	TCATGA	1 cut(s)	1488
CfoI	GCGC	8 cut(s)	559, 1228, 1353, 1844, 2492, 2712, 2980, 3178
Cfr10I	RCCGGY	1 cut(s)	3322
Cfr13I	GGNCC	2 cut(s)	101, 2836
CseI	GACGC	1 cut(s)	1937
CspCI	CAANNNNNGTGG	2 cut(s)	565, 600
DdeI	CTNAG	9 cut(s)	192, 873, 1019, 1378, 2007, 2154, 2234, 2397, 2443
DrdI	GACNNNNNNGTC	1 cut(s)	2927
DseDI	GACNNNNNNGTC	1 cut(s)	2927
EaeI	YGGCCR	4 cut(s)	1255, 2577, 2728, 2934
Eam1104I	CTCTTC	6 cut(s)	1407, 1591, 1806, 1887, 2382, 3218
EarI	CTCTTC	6 cut(s)	1407, 1591, 1806, 1887, 2382, 3218
EciI	GGCGGA	1 cut(s)	298
Eco130I	CCWWGG	4 cut(s)	219, 2019, 2322, 2907
Eco147I	AGGCCT	1 cut(s)	3086
Eco31I	GGTCTC	1 cut(s)	566
Eco32I	GATATC	1 cut(s)	2626
Eco47III	AGCGCT	1 cut(s)	558
Eco57I	CTGAAG	3 cut(s)	1145, 1446, 1596
Eco88I	CYCGRG	4 cut(s)	112, 1221, 2833, 3470
EcoNI	CCTNNNNNAGG	1 cut(s)	1381
EcoRI	GAATTC	3 cut(s)	853, 2000, 2425
EcoRII	CCWGG	5 cut(s)	103, 168, 987, 1382, 1901
EcoRV	GATATC	1 cut(s)	2626
EcoT14I	CCWWGG	4 cut(s)	219, 2019, 2322, 2907
EcoT22I	ATGCAT	1 cut(s)	2141
ErhI	CCWWGG	4 cut(s)	219, 2019, 2322, 2907
Esp3I	CGTCTC	1 cut(s)	2520
FalI	AAGNNNNNCTT	4 cut(s)	2100, 2132, 2450, 2482
FaqI	GGGAC	2 cut(s)	311, 2090
FauI	CCCGC	3 cut(s)	2964, 3092, 3539
FauNDI	CATATG	2 cut(s)	1606, 3259
FbaI	TGATCA	3 cut(s)	1005, 1797, 2412
FblI	GTMKAC	1 cut(s)	197
FokI	GGATG	5 cut(s)	910, 1004, 1490, 3027, 3383
FspBI	CTAG	4 cut(s)	978, 1550, 2471, 3047
FspI	TGCGCA	2 cut(s)	2491, 2979
GlaI	GCGC	8 cut(s)	558, 1227, 1352, 1843, 2491, 2711, 2979, 3177
GsuI	CTGGAG	1 cut(s)	2205
HaeII	RGCGCY	4 cut(s)	560, 1845, 2713, 3179
HaeIII	GGCC	9 cut(s)	102, 152, 1257, 2012, 2579, 2730, 2838, 2936, 3086
HapII	CCGG	3 cut(s)	2897, 3004, 3323
HgaI	GACGC	1 cut(s)	1937
HhaI	GCGC	8 cut(s)	559, 1228, 1353, 1844, 2492, 2712, 2980, 3178
Hin6I	GCGC	8 cut(s)	557, 1226, 1351, 1842, 2490, 2710, 2978, 3176
HinP1I	GCGC	8 cut(s)	557, 1226, 1351, 1842, 2490, 2710, 2978, 3176
HincII	GTYRAC	1 cut(s)	3163
HindII	GTYRAC	1 cut(s)	3163
HindIII	AAGCTT	3 cut(s)	66, 652, 2147
HinfI	GANTC	7 cut(s)	42, 647, 779, 1616, 1681, 2083, 2748
HpaII	CCGG	3 cut(s)	2897, 3004, 3323
HphI	GGTGA	6 cut(s)	449, 1406, 1611, 2035, 2597, 3046
Hpy166II	GTNNAC	7 cut(s)	124, 198, 1042, 1167, 1189, 3163, 3320
Hpy8I	GTNNAC	7 cut(s)	124, 198, 1042, 1167, 1189, 3163, 3320
Hpy99I	CGWCG	3 cut(s)	326, 338, 341
HpyCH4IV	ACGT	3 cut(s)	321, 2888, 2985
HpyF10VI	GCNNNNNNNGC	8 cut(s)	171, 298, 769, 1067, 1340, 2375, 2537, 3263
HpyF3I	CTNAG	9 cut(s)	192, 873, 1019, 1378, 2007, 2154, 2234, 2397, 2443
HpySE526I	ACGT	3 cut(s)	321, 2888, 2985
HspAI	GCGC	8 cut(s)	557, 1226, 1351, 1842, 2490, 2710, 2978, 3176
Kpn2I	TCCGGA	1 cut(s)	3003
KpnI	GGTACC	1 cut(s)	1018
Ksp22I	TGATCA	3 cut(s)	1005, 1797, 2412
LmnI	GCTCC	5 cut(s)	2270, 2707, 3006, 3263, 3331
Lsp1109I	GCAGC	9 cut(s)	40, 774, 775, 1082, 1643, 1658, 2365, 2474, 3404
LweI	GCATC	5 cut(s)	439, 1701, 2501, 3237, 3543
MaeI	CTAG	4 cut(s)	978, 1550, 2471, 3047
MaeII	ACGT	3 cut(s)	321, 2888, 2985
MfeI	CAATTG	1 cut(s)	2685
MflI	RGATCY	5 cut(s)	862, 1416, 1711, 2461, 3497
MhlI	GDGCHC	4 cut(s)	1063, 1169, 1191, 3322
MlsI	TGGCCA	2 cut(s)	1257, 2730
MluNI	TGGCCA	2 cut(s)	1257, 2730
MlyI	GAGTC	3 cut(s)	641, 773, 1675
MmeI	TCCRAC	2 cut(s)	1076, 2584
Mox20I	TGGCCA	2 cut(s)	1257, 2730
Mph1103I	ATGCAT	1 cut(s)	2141
MroI	TCCGGA	1 cut(s)	3003
MscI	TGGCCA	2 cut(s)	1257, 2730
MslI	CAYNNNNRTG	4 cut(s)	404, 435, 1521, 3401
Msp20I	TGGCCA	2 cut(s)	1257, 2730
MspA1I	CMGCKG	1 cut(s)	2643
MspI	CCGG	3 cut(s)	2897, 3004, 3323
MspR9I	CCNGG	5 cut(s)	105, 170, 989, 1384, 1903
MunI	CAATTG	1 cut(s)	2685
Mva1269I	GAATGC	5 cut(s)	85, 146, 2141, 2613, 2661
MvaI	CCWGG	5 cut(s)	105, 170, 989, 1384, 1903
MvnI	CGCG	3 cut(s)	1228, 1353, 1962
MwoI	GCNNNNNNNGC	8 cut(s)	171, 298, 769, 1067, 1340, 2375, 2537, 3263
NdeI	CATATG	2 cut(s)	1606, 3259
NlaIV	GGNNCC	8 cut(s)	1016, 1062, 1713, 2130, 3002, 3130, 3265, 3327
NmeAIII	GCCGAG	1 cut(s)	2912
NmuCI	GTSAC	5 cut(s)	830, 2865, 2889, 3034, 3361
NruI	TCGCGA	1 cut(s)	1962
NsbI	TGCGCA	2 cut(s)	2491, 2979
NsiI	ATGCAT	1 cut(s)	2141
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	1 cut(s)	1860
PaeR7I	CTCGAG	1 cut(s)	112
PagI	TCATGA	1 cut(s)	1488
PceI	AGGCCT	1 cut(s)	3086
PciI	ACATGT	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	2 cut(s)	330, 504
PctI	GAATGC	5 cut(s)	85, 146, 2141, 2613, 2661
PfeI	GAWTC	4 cut(s)	42, 1616, 2083, 2748
PflFI	GACNNNGTC	1 cut(s)	1850
PleI	GAGTC	3 cut(s)	641, 773, 1675
PpsI	GAGTC	3 cut(s)	641, 773, 1675
PscI	ACATGT	1 cut(s)	1191
PshBI	ATTAAT	1 cut(s)	2208
PsiI	TTATAA	1 cut(s)	1175
Psp6I	CCWGG	5 cut(s)	103, 168, 987, 1382, 1901
PspGI	CCWGG	5 cut(s)	103, 168, 987, 1382, 1901
PspN4I	GGNNCC	8 cut(s)	1016, 1062, 1713, 2130, 3002, 3130, 3265, 3327
PspPI	GGNCC	2 cut(s)	101, 2836
PstI	CTGCAG	2 cut(s)	2104, 2278
PstNI	CAGNNNCTG	2 cut(s)	769, 2404
PsuI	RGATCY	5 cut(s)	862, 1416, 1711, 2461, 3497
PsyI	GACNNNGTC	1 cut(s)	1850
RruI	TCGCGA	1 cut(s)	1962
RseI	CAYNNNNRTG	4 cut(s)	404, 435, 1521, 3401
Sau96I	GGNCC	2 cut(s)	101, 2836
ScaI	AGTACT	1 cut(s)	976
SchI	GAGTC	3 cut(s)	641, 773, 1675
ScrFI	CCNGG	5 cut(s)	105, 170, 989, 1384, 1903
SduI	GDGCHC	4 cut(s)	1063, 1169, 1191, 3322
SfaNI	GCATC	5 cut(s)	439, 1701, 2501, 3237, 3543
SfcI	CTRYAG	3 cut(s)	1541, 2100, 2274
Sfr274I	CTCGAG	1 cut(s)	112
SfuI	TTCGAA	1 cut(s)	1860
SlaI	CTCGAG	1 cut(s)	112
SmiMI	CAYNNNNRTG	4 cut(s)	404, 435, 1521, 3401
SmlI	CTYRAG	5 cut(s)	112, 649, 1683, 1777, 2948
SmoI	CTYRAG	5 cut(s)	112, 649, 1683, 1777, 2948
SpeI	ACTAGT	1 cut(s)	977
SseBI	AGGCCT	1 cut(s)	3086
SspI	AATATT	1 cut(s)	2212
SspMI	CTAG	4 cut(s)	978, 1550, 2471, 3047
StuI	AGGCCT	1 cut(s)	3086
StyD4I	CCNGG	5 cut(s)	103, 168, 987, 1382, 1901
StyI	CCWWGG	4 cut(s)	219, 2019, 2322, 2907
TaiI	ACGT	3 cut(s)	324, 2891, 2988
TaqI	TCGA	7 cut(s)	113, 498, 775, 1307, 1451, 1860, 3537
TatI	WGTACW	5 cut(s)	123, 705, 974, 2722, 3138
TauI	GCSGC	3 cut(s)	102, 295, 3493
TfiI	GAWTC	4 cut(s)	42, 1616, 2083, 2748
TseFI	GTSAC	5 cut(s)	830, 2865, 2889, 3034, 3361
TseI	GCWGC	9 cut(s)	28, 763, 787, 1070, 1631, 1646, 2378, 2487, 3392
Tsp45I	GTSAC	5 cut(s)	830, 2865, 2889, 3034, 3361
TspGWI	ACGGA	6 cut(s)	106, 812, 1509, 1783, 1893, 3318
Tth111I	GACNNNGTC	1 cut(s)	1850
VneI	GTGCAC	3 cut(s)	1165, 1187, 3318
VspI	ATTAAT	1 cut(s)	2208
XagI	CCTNNNNNAGG	1 cut(s)	1381
XapI	RAATTY	8 cut(s)	445, 661, 700, 853, 1313, 1667, 2000, 2425
XbaI	TCTAGA	1 cut(s)	2470
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3257
XhoI	CTCGAG	1 cut(s)	112
XmiI	GTMKAC	1 cut(s)	197
XspI	CTAG	4 cut(s)	978, 1550, 2471, 3047
ZrmI	AGTACT	1 cut(s)	976
Zsp2I	ATGCAT	1 cut(s)	2141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.