Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Reverse (-)

4575551 .. 4581328

Gene Structure 5778 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G005410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3591 bp

ATGGGTGGTGGTGGTAGAAGGAAGAGGCAGCATTTTGGGAGAATCCATGCCTTTCACCGTGGAAAACCATCCGTAGATGAGGAGCATTCGCAGATCGGAGGCCCCGGATTCTCTAGAGTAGTCCATTGCAATGACCCTGAATGCCTTGAGGCCACTGCTAAGAACTATGAAAGCAATTATGTTAGAACCACCAAGTATACACTCGCTACATTCTTACCTAAGGCAGTGTTTGAGCAATTCAGAAGGGTTGCAAATCTGTATTTCCTCATTTGTGCAATACTGTCATTTACACCGCTTTCGCCTTACTCTGCTGTCAGTAATGTTGTCCCTCTTGTGGTTGTGGTTGGAGTTACAATGGGAAAAGAGGCCCTTGAAGATTGGCGGCGCAAAAAGCAGGATATGGATGTGAACAACAGAAAAGTTCTGGTGCATCGATCGGAAGGTGAATTTGATTTTACCAAGTGGAGGGATTTGAAAGTTGGGGATATAGTGAAAGTGGAAAAGGACCAATTTTTTCCTGCTGATCTTATCTTACTTTCATCAAGCTATGATGAAGCACTTTGCTATGTAGAGACCACCAACCTTGATGGGGAAACTAATTTGAAAATAAAACAATCTCTGGAAGCAACTTCTAATCTTCATGAAGACTCGGGCTTCAAAGATTTCAAGGCTTTGATCAGATGTGAAGACCCGAATGCAAGTCTGTACTCATTCGTAGGGAGTCTGGAGTTTGAAGGGGAATCATACCCTCTTTCACCTCAGCAGCTTCTGCTTAGGGACTCTAAGCTGAGAAACACTGATTTTATATATGGGGTTGTAATCTTCACAGGTCATGATACAAAAGTTATGCAAAACTCAACTGAACCACCTTCCAAGAGAAGCAAAATTGAGAAAAGGATGGATAAGATAGTCTACTTCTTGTTTTTCCTCTTAGTTGTGATGTCTTTTGTTGGTGCTATTGTCTTTGGGGTTACCACTAGCGAAGACATAGAAGATGGGCAATTGATAAGATGGTACCTTAGACCAGATGATACTACAGTTTACTATGATCCAACAAGAGCGCCACTTGCAGCCATTTTGCAGTTCTTAACAGCCGTTATGTTGTACAGTTATTTGATTCCCATTTCCTTGTATGTGTCGATAGAGATTGTCAAAGTGCTCCAGAGCATTTTCATCAACCAGGATCTGCACATGTACTATGAGGAAACTGACAAGCCAGCTCGAGCACGTACCTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATTCTATCTGACAAAACAGGAACCTTAACTTGCAACTCAATGGAGTTTATCAAGTGTTCTATTGCCGGGACTGCTTTTGGGCGCGGAGTTACAGAAGTTGAGAGAGCTTTGTCCAGTGGCAAAGGCTCATCTTTGTTTGAAGGGGTGACAGAAGAAGAAGGCCAAGTTGAAGAATCTACTGAAGCCAAGACATCAATAAAAGGCTTTAACTTCATGGATGAAAGAATAATGGATGGCAATTGGGTTAAGGAGCCTCATGCAGATGTAATCCAGAAGTTTCTACAGTTACTCGCCGTCTGTCATACAGCAATACCTGAAGTTGATGAAGAATCTGGAAGAGTTTCATATGAGGCTGAATCACCAGATGAGGCAGCTTTTGTGATTGCAGCAAGAGAGCTTGGTTTTGAATTTTATGAAAGGGCTCAAACAAGCATCTCCCTGCACGAGTTTGATCCAATGTCTGGAAGGAGAGTTGAAAGATCTTATAAGATTTTGAATGTATTGGAGTTTAGTAGCTCAAGGAAGCGAATGTCTGTGATTGTAAGAAATGAGGAGGGAAAGATACTATTGCTCAGTAAAGGTGCTGACAGTGTCATGTTCCAAAGACTTGCAAAGGATGGAAGGGAGTTTGAAGAGAAGACCCGGGAGCACATTAACGAGTATGCTGATGCTGGTTTGAGGACTTTAGTACTTGCATATCGGGAACTTGATGAAGAAGAGTATTTTGAATTTAACAAAGAATATACTGAGGCCAAGAACTTGGTTAGTTCAGATCGAGAGGAAGCTGTCGAGGAAGTGGCTGAGAAGATAGAGAGGAACTTGATTCTTCTTGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCTGACTGCATTGACAAGCTTGCTCAGGCTGGAATTAAACTATGGGTTTTGACTGGAGATAAAATGGAGACAGCAATTAATATTGGATATGCCTGCAGTTTGCTTCGACAAGGAATGAAACAAATAATAATAAGCTCAGAAACTCCAGAAGCTAAAGCATTAGACAAGGTGGAGGACAAGTCCAAAGTTGCCATGGCATTGAAGGAAAGTGTTTTCCATCAAATAAGTGAGGCAAAGGCATATCTTGCTTCACTAGATCAGAACTCCGAAGCATTGGCTTTGATCATTGATGGGAATTCACTTGCTTATGCCTTAGAGGTAGATGTCCAGGACCTCTTTCTAGAGCTTGCCATTGGCTGTGCATCTGTTATATGCTGTCGGTCATCTCCCAAACAGAAAGCACTTGTCACAAGACTGGTAAAAATTAAAACTGGTAAAACAACTCTGGCAATTGGTGATGGGGCAAATGATGTAGGAATGCTTCAAGAAGCAGATATTGGTGTTGGTATCAGCGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGTGACATTGCCATTGCACAGTTTCGTTTTCTGGAGCGCCTGCTACTCGTACATGGACATTGGTGTTACAGAAGAATCTCATCAATGATATGCTATTTCTTTTACAAGAACATTACTTTTGGCTTCACTATCTTTTTCTATGAGATGTATGCTTCATTCTCGGGTCAATCTGCATATAATGATTGGTATCTATCACTCTATAATGTCTTCTTCACATCACTTCCTGTAATTGCATTGGGAGTGTTTGACCAAGATGTCTCAGCCAGATTCTGCCTCAAGTTCCCACTCTTGTACCAAGAGGGTGCACAAAATGTCTTATTTAGCTGGGTCCGGATACTTGGATGGTCGCTGAACGGCATTGTGTCTGCTATGATAATCTTCTTCTGCTGCATCCTGGCAATAGGTAGTCAAGCCTTTCGCAAAGGAGGGGAAGTTGTTGGCTTCGAAATCTTTGGCGCCACAATGTACTCAATTGTGGTATGGGTTGTCAACTGTCAGATGGCACTATCCATCAGTTACTTCACTTACATACAACATCTCTTCATCTGGGGTGGCATTGTGTTCTGGTACATATTCCAGCTGGCATATGGAGCCTTGGACCCTTCCATATCAACCACTGCCTATAAGGTCTTCATTGAAGCCTGTGCACCAGCCCCATTTTATTGGCTTCTCACACTCTTGGTAGTAGTCACTTCTCTCCTACCTTATTTCACTTATGCCGCCTTCCAAATGCGGTTCTTCCCCATGTATCATCAGATGATACAATGGATGAGAACAGATGGGCAATCAGATGACCCTGAGTTCTGTCGCATGGTGAGGCAGAGATCGATAAGACCCACAACAGTAGGCTACACAGCTCGCATTGCAACATCTAAACGCTTTGAAGAGAAGCCCGAGAGTAGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1196

Amino Acids

135.2

Weight (kDa)

5.29

Isoelectric Point (pI)

41.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 106	6.4e-24	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	514 - 710	3.6e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	892 - 1142	4e-87	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1755
AasI	GACNNNNNNGTC	1 cut(s)	2936
Acc65I	GGTACC	1 cut(s)	1014
AccB1I	GGYRCC	2 cut(s)	1014, 3137
AccB7I	CCANNNNNTGG	1 cut(s)	1730
AccI	GTMKAC	2 cut(s)	197, 912
AccII	CGCG	1 cut(s)	1353
AccIII	TCCGGA	1 cut(s)	3012
AciI	CCGC	6 cut(s)	293, 382, 1353, 2652, 3402, 3415
AclWI	GGATC	3 cut(s)	1043, 1191, 1715
AcoI	YGGCCR	1 cut(s)	1255
AcsI	RAATTY	4 cut(s)	446, 1676, 1997, 2434
AcuI	CTGAAG	2 cut(s)	1470, 1605
AcyI	GRCGYC	1 cut(s)	3138
AfiI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1730
AflIII	ACRYGT	1 cut(s)	1191
AjnI	CCWGG	3 cut(s)	1179, 2466, 3075
AjuI	GAANNNNNNNTTGG	2 cut(s)	2619, 2651
Alw21I	GWGCWC	5 cut(s)	1161, 1228, 1920, 2989, 3331
Alw26I	GTCTC	3 cut(s)	566, 2201, 2944
Alw44I	GTGCAC	2 cut(s)	2985, 3327
AlwI	GGATC	3 cut(s)	1043, 1191, 1715
AlwNI	CAGNNNCTG	3 cut(s)	769, 1186, 2952
Ama87I	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
Aor13HI	TCCGGA	1 cut(s)	3012
AoxI	GGCC	6 cut(s)	100, 150, 366, 1255, 1429, 2019
ApaLI	GTGCAC	2 cut(s)	2985, 3327
ApeKI	GCWGC	6 cut(s)	28, 763, 1070, 1640, 1655, 3069
ApoI	RAATTY	4 cut(s)	446, 1676, 1997, 2434
AseI	ATTAAT	1 cut(s)	2217
Asp700I	GAANNNNTTC	1 cut(s)	1609
Asp718I	GGTACC	1 cut(s)	1014
AspLEI	GCGC	5 cut(s)	387, 1063, 1353, 2721, 3140
AspS9I	GGNCC	6 cut(s)	101, 367, 505, 2470, 3010, 3280
AsuC2I	CCSGG	4 cut(s)	105, 1336, 1912, 1913
AsuHPI	GGTGA	7 cut(s)	47, 455, 747, 1426, 1620, 2606, 3508
AsuII	TTCGAA	1 cut(s)	3126
AvaI	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
AvaII	GGWCC	4 cut(s)	505, 2470, 3010, 3280
AxyI	CCTNAGG	1 cut(s)	219
BaeGI	GKGCMC	2 cut(s)	2989, 3331
BaeI	ACNNNNGTAYC	6 cut(s)	828, 861, 1023, 1056, 2957, 2990
BalI	TGGCCA	1 cut(s)	1257
BanI	GGYRCC	2 cut(s)	1014, 3137
BanII	GRGCYC	1 cut(s)	1693
BarI	GAAGNNNNNNTAC	2 cut(s)	2604, 2636
BauI	CACGAG	1 cut(s)	1712
BbsI	GAAGAC	7 cut(s)	651, 693, 990, 1913, 2124, 2881, 3304
Bbv12I	GWGCWC	5 cut(s)	1161, 1228, 1920, 2989, 3331
BbvCI	CCTCAGC	1 cut(s)	759
BbvI	GCAGC	6 cut(s)	40, 775, 1082, 1652, 1667, 3056
BceAI	ACGGC	3 cut(s)	1079, 1547, 3052
BciT130I	CCWGG	3 cut(s)	1181, 2468, 3077
BciVI	GTATCC	1 cut(s)	3009
BclI	TGATCA	2 cut(s)	675, 2421
BcnI	CCSGG	4 cut(s)	105, 1336, 1912, 1913
BcoDI	GTCTC	3 cut(s)	566, 2201, 2944
BfaI	CTAG	4 cut(s)	114, 978, 2393, 2480
BfmI	CTRYAG	4 cut(s)	1035, 1550, 2109, 2233
BfoI	RGCGCY	3 cut(s)	1064, 2722, 3141
BfuI	GTATCC	1 cut(s)	3009
BglII	AGATCT	1 cut(s)	1748
BisI	GCNGC	8 cut(s)	29, 383, 764, 1071, 1641, 1656, 3070, 3402
BlsI	GCNGC	8 cut(s)	30, 384, 765, 1072, 1642, 1657, 3071, 3403
BmcAI	AGTACT	1 cut(s)	1959
Bme1390I	CCNGG	7 cut(s)	105, 1181, 1336, 1912, 1913, 2468, 3077
Bme18I	GGWCC	4 cut(s)	505, 2470, 3010, 3280
BmeT110I	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
BmgT120I	GGNCC	6 cut(s)	101, 367, 505, 2470, 3010, 3280
BmiI	GGNNCC	9 cut(s)	103, 1016, 1291, 1521, 2139, 3011, 3139, 3274, 3282
BmrFI	CCNGG	7 cut(s)	105, 1181, 1336, 1912, 1913, 2468, 3077
BmsI	GCATC	5 cut(s)	439, 1710, 1927, 2510, 3081
BpiI	GAAGAC	7 cut(s)	651, 693, 990, 1913, 2124, 2881, 3304
BpmI	CTGGAG	5 cut(s)	746, 1145, 2214, 2268, 2735
Bpu10I	CCTNAGC	3 cut(s)	759, 773, 2163
Bpu14I	TTCGAA	1 cut(s)	3126
BpuEI	CTTGAG	3 cut(s)	167, 1771, 2942
BpuMI	CCSGG	4 cut(s)	105, 1336, 1912, 1913
Bsa29I	ATCGAT	2 cut(s)	433, 3509
BsaAI	YACGTR	1 cut(s)	1229
BsaBI	GATNNNNATC	1 cut(s)	2868
BsaHI	GRCGYC	1 cut(s)	3138
BsaI	GGTCTC	1 cut(s)	566
BsaJI	CCNNGG	5 cut(s)	58, 103, 1911, 2331, 3276
BsaWI	WCCGGW	1 cut(s)	3012
BsaXI	ACNNNNNCTCC	4 cut(s)	2303, 2333, 3099, 3129
Bsc4I	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1730
Bse1I	ACTGG	4 cut(s)	1383, 2197, 2559, 2575
Bse21I	CCTNAGG	1 cut(s)	219
Bse3DI	GCAATG	5 cut(s)	124, 136, 2688, 2694, 3543
Bse8I	GATNNNNATC	1 cut(s)	2868
BseAI	TCCGGA	1 cut(s)	3012
BseBI	CCWGG	3 cut(s)	1181, 2468, 3077
BseCI	ATCGAT	2 cut(s)	433, 3509
BseDI	CCNNGG	5 cut(s)	58, 103, 1911, 2331, 3276
BseGI	GGATG	9 cut(s)	68, 409, 903, 1492, 1507, 1891, 3029, 3072, 3456
BseJI	GATNNNNATC	1 cut(s)	2868
BseLI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1730
BseMI	GCAATG	5 cut(s)	124, 136, 2688, 2694, 3543
BseMII	CTCAG	9 cut(s)	773, 779, 1855, 2007, 2061, 2177, 2289, 2955, 3471
BseNI	ACTGG	4 cut(s)	1383, 2197, 2559, 2575
BseRI	GAGGAG	2 cut(s)	95, 1835
BseSI	GKGCMC	2 cut(s)	2989, 3331
BseXI	GCAGC	6 cut(s)	40, 775, 1082, 1652, 1667, 3056
BseYI	CCCAGC	1 cut(s)	3006
BsgI	GTGCAG	2 cut(s)	1172, 1694
Bsh1236I	CGCG	1 cut(s)	1353
Bsh1285I	CGRYCG	1 cut(s)	437
BshFI	GGCC	6 cut(s)	102, 152, 368, 1257, 1431, 2021
BshNI	GGYRCC	2 cut(s)	1014, 3137
BshVI	ATCGAT	2 cut(s)	433, 3509
BsiEI	CGRYCG	1 cut(s)	437
BsiHKAI	GWGCWC	5 cut(s)	1161, 1228, 1920, 2989, 3331
BsiHKCI	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
BsiSI	CCGG	4 cut(s)	105, 1335, 1912, 3013
BslFI	GGGAC	3 cut(s)	311, 791, 1351
BslI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1730
BsmAI	GTCTC	3 cut(s)	566, 2201, 2944
BsmFI	GGGAC	3 cut(s)	311, 791, 1351
BsmI	GAATGC	5 cut(s)	85, 146, 700, 2622, 2670
BsnI	GGCC	6 cut(s)	102, 152, 368, 1257, 1431, 2021
Bso31I	GGTCTC	1 cut(s)	566
BsoBI	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
Bsp119I	TTCGAA	1 cut(s)	3126
Bsp1286I	GDGCHC	6 cut(s)	1161, 1228, 1693, 1920, 2989, 3331
Bsp13I	TCCGGA	1 cut(s)	3012
Bsp1407I	TGTACA	1 cut(s)	1104
Bsp19I	CCATGG	1 cut(s)	2331
BspACI	CCGC	6 cut(s)	293, 382, 1353, 2652, 3402, 3415
BspANI	GGCC	6 cut(s)	102, 152, 368, 1257, 1431, 2021
BspCNI	CTCAG	9 cut(s)	772, 780, 1854, 2008, 2062, 2176, 2288, 2954, 3472
BspDI	ATCGAT	2 cut(s)	433, 3509
BspEI	TCCGGA	1 cut(s)	3012
BspFNI	CGCG	1 cut(s)	1353
BspHI	TCATGA	2 cut(s)	640, 832
BspLI	GGNNCC	9 cut(s)	103, 1016, 1291, 1521, 2139, 3011, 3139, 3274, 3282
BspMAI	CTGCAG	2 cut(s)	2113, 2237
BspPI	GGATC	3 cut(s)	1043, 1191, 1715
BspT104I	TTCGAA	1 cut(s)	3126
BspT107I	GGYRCC	2 cut(s)	1014, 3137
BspTNI	GGTCTC	1 cut(s)	566
BsrDI	GCAATG	5 cut(s)	124, 136, 2688, 2694, 3543
BsrGI	TGTACA	1 cut(s)	1104
BsrI	ACTGG	4 cut(s)	1383, 2197, 2559, 2575
BssECI	CCNNGG	5 cut(s)	58, 103, 1911, 2331, 3276
BssNAI	GTATAC	1 cut(s)	198
BssNI	GRCGYC	1 cut(s)	3138
BssSI	CACGAG	1 cut(s)	1712
BssT1I	CCWWGG	2 cut(s)	2331, 3276
Bst1107I	GTATAC	1 cut(s)	198
Bst2BI	CACGAG	1 cut(s)	1712
Bst2UI	CCWGG	3 cut(s)	1181, 2468, 3077
Bst4CI	ACNGT	9 cut(s)	59, 282, 1039, 1109, 1554, 1859, 2703, 3176, 3526
Bst6I	CTCTTC	6 cut(s)	17, 1600, 1896, 1980, 3227, 3561
BstACI	GRCGYC	1 cut(s)	3138
BstAPI	GCANNNNNTGC	2 cut(s)	769, 2384
BstAUI	TGTACA	1 cut(s)	1104
BstBAI	YACGTR	1 cut(s)	1229
BstBI	TTCGAA	1 cut(s)	3126
BstC8I	GCNNGC	8 cut(s)	1218, 2160, 2233, 2487, 2670, 2723, 3264, 3541
BstDSI	CCRYGG	2 cut(s)	58, 2331
BstEII	GGTNACC	1 cut(s)	970
BstF5I	GGATG	9 cut(s)	68, 409, 903, 1492, 1507, 1891, 3029, 3072, 3456
BstFNI	CGCG	1 cut(s)	1353
BstH2I	RGCGCY	3 cut(s)	1064, 2722, 3141
BstHHI	GCGC	5 cut(s)	387, 1063, 1353, 2721, 3140
BstMAI	GTCTC	3 cut(s)	566, 2201, 2944
BstMCI	CGRYCG	1 cut(s)	437
BstMWI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2384, 3272, 3545
BstNI	CCWGG	3 cut(s)	1181, 2468, 3077
BstNSI	RCATGY	1 cut(s)	1195
BstPI	GGTNACC	1 cut(s)	970
BstSCI	CCNGG	7 cut(s)	103, 1179, 1334, 1910, 1911, 2466, 3075
BstSFI	CTRYAG	4 cut(s)	1035, 1550, 2109, 2233
BstSLI	GKGCMC	2 cut(s)	2989, 3331
BstUI	CGCG	1 cut(s)	1353
BstV1I	GCAGC	6 cut(s)	40, 775, 1082, 1652, 1667, 3056
BstV2I	GAAGAC	7 cut(s)	651, 693, 990, 1913, 2124, 2881, 3304
BstX2I	RGATCY	2 cut(s)	1183, 1748
BstXI	CCANNNNNNTGG	2 cut(s)	2029, 3345
BstYI	RGATCY	2 cut(s)	1183, 1748
BstZ17I	GTATAC	1 cut(s)	198
Bsu15I	ATCGAT	2 cut(s)	433, 3509
Bsu36I	CCTNAGG	1 cut(s)	219
BsuI	GTATCC	1 cut(s)	3009
BsuRI	GGCC	6 cut(s)	102, 152, 368, 1257, 1431, 2021
BsuTUI	ATCGAT	2 cut(s)	433, 3509
BtgI	CCRYGG	2 cut(s)	58, 2331
BtsCI	GGATG	9 cut(s)	68, 409, 903, 1492, 1507, 1891, 3029, 3072, 3456
BtsI	GCAGTG	3 cut(s)	153, 231, 3297
BtsIMutI	CAGTG	6 cut(s)	153, 231, 795, 1390, 1864, 3297
Cac8I	GCNNGC	8 cut(s)	1218, 2160, 2233, 2487, 2670, 2723, 3264, 3541
CaiI	CAGNNNCTG	3 cut(s)	769, 1186, 2952
CciI	TCATGA	2 cut(s)	640, 832
CfoI	GCGC	5 cut(s)	387, 1063, 1353, 2721, 3140
Cfr13I	GGNCC	6 cut(s)	101, 367, 505, 2470, 3010, 3280
Cfr9I	CCCGGG	1 cut(s)	1911
ClaI	ATCGAT	2 cut(s)	433, 3509
CspCI	CAANNNNNGTGG	2 cut(s)	565, 600
DinI	GGCGCC	1 cut(s)	3139
DrdI	GACNNNNNNGTC	1 cut(s)	2936
DseDI	GACNNNNNNGTC	1 cut(s)	2936
EaeI	YGGCCR	1 cut(s)	1255
Eam1104I	CTCTTC	6 cut(s)	17, 1600, 1896, 1980, 3227, 3561
EarI	CTCTTC	6 cut(s)	17, 1600, 1896, 1980, 3227, 3561
Eco130I	CCWWGG	2 cut(s)	2331, 3276
Eco24I	GRGCYC	1 cut(s)	1693
Eco31I	GGTCTC	1 cut(s)	566
Eco47I	GGWCC	4 cut(s)	505, 2470, 3010, 3280
Eco57I	CTGAAG	2 cut(s)	1470, 1605
Eco81I	CCTNAGG	1 cut(s)	219
Eco88I	CYCGRG	5 cut(s)	649, 1221, 1911, 2842, 3575
Eco91I	GGTNACC	1 cut(s)	970
EcoO109I	RGGNCCY	3 cut(s)	101, 367, 2470
EcoO65I	GGTNACC	1 cut(s)	970
EcoRI	GAATTC	1 cut(s)	2434
EcoRII	CCWGG	3 cut(s)	1179, 2466, 3075
EcoT14I	CCWWGG	2 cut(s)	2331, 3276
EcoT38I	GRGCYC	1 cut(s)	1693
EgeI	GGCGCC	1 cut(s)	3139
EheI	GGCGCC	1 cut(s)	3139
ErhI	CCWWGG	2 cut(s)	2331, 3276
FalI	AAGNNNNNCTT	4 cut(s)	354, 386, 2109, 2141
FaqI	GGGAC	3 cut(s)	311, 791, 1351
FauNDI	CATATG	2 cut(s)	1615, 3268
FbaI	TGATCA	2 cut(s)	675, 2421
FblI	GTMKAC	2 cut(s)	197, 912
Fnu4HI	GCNGC	8 cut(s)	29, 383, 764, 1071, 1641, 1656, 3070, 3402
FokI	GGATG	9 cut(s)	55, 416, 910, 1499, 1514, 1898, 3036, 3059, 3463
FriOI	GRGCYC	1 cut(s)	1693
Fsp4HI	GCNGC	8 cut(s)	29, 383, 764, 1071, 1641, 1656, 3070, 3402
FspBI	CTAG	4 cut(s)	114, 978, 2393, 2480
GlaI	GCGC	5 cut(s)	386, 1062, 1352, 2720, 3139
GluI	GCNGC	8 cut(s)	29, 383, 764, 1071, 1641, 1656, 3070, 3402
GsaI	CCCAGC	1 cut(s)	3010
GsuI	CTGGAG	5 cut(s)	746, 1145, 2214, 2268, 2735
HaeII	RGCGCY	3 cut(s)	1064, 2722, 3141
HaeIII	GGCC	6 cut(s)	102, 152, 368, 1257, 1431, 2021
HapII	CCGG	4 cut(s)	105, 1335, 1912, 3013
HhaI	GCGC	5 cut(s)	387, 1063, 1353, 2721, 3140
Hin1I	GRCGYC	1 cut(s)	3138
Hin6I	GCGC	5 cut(s)	385, 1061, 1351, 2719, 3138
HinP1I	GCGC	5 cut(s)	385, 1061, 1351, 2719, 3138
HincII	GTYRAC	1 cut(s)	3172
HindII	GTYRAC	1 cut(s)	3172
HindIII	AAGCTT	1 cut(s)	2156
HpaII	CCGG	4 cut(s)	105, 1335, 1912, 3013
HphI	GGTGA	7 cut(s)	47, 455, 747, 1426, 1620, 2606, 3508
Hpy166II	GTNNAC	7 cut(s)	198, 409, 913, 1042, 2987, 3172, 3329
Hpy8I	GTNNAC	7 cut(s)	198, 409, 913, 1042, 2987, 3172, 3329
HpyCH4III	ACNGT	9 cut(s)	59, 282, 1039, 1109, 1554, 1859, 2703, 3176, 3526
HpyCH4IV	ACGT	1 cut(s)	1228
HpyF10VI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2384, 3272, 3545
HpySE526I	ACGT	1 cut(s)	1228
Hsp92I	GRCGYC	1 cut(s)	3138
HspAI	GCGC	5 cut(s)	385, 1061, 1351, 2719, 3138
KasI	GGCGCC	1 cut(s)	3137
Kpn2I	TCCGGA	1 cut(s)	3012
KpnI	GGTACC	1 cut(s)	1018
Ksp22I	TGATCA	2 cut(s)	675, 2421
LmnI	GCTCC	6 cut(s)	82, 1164, 1519, 1915, 2716, 3272
Lsp1109I	GCAGC	6 cut(s)	40, 775, 1082, 1652, 1667, 3056
LweI	GCATC	5 cut(s)	439, 1710, 1927, 2510, 3081
MaeI	CTAG	4 cut(s)	114, 978, 2393, 2480
MaeII	ACGT	1 cut(s)	1228
MfeI	CAATTG	4 cut(s)	1001, 1507, 2589, 3153
MflI	RGATCY	2 cut(s)	1183, 1748
MhlI	GDGCHC	6 cut(s)	1161, 1228, 1693, 1920, 2989, 3331
MlsI	TGGCCA	1 cut(s)	1257
MluNI	TGGCCA	1 cut(s)	1257
Mly113I	GGCGCC	1 cut(s)	3138
MlyI	GAGTC	3 cut(s)	641, 730, 773
MmeI	TCCRAC	2 cut(s)	325, 1076
Mox20I	TGGCCA	1 cut(s)	1257
MroI	TCCGGA	1 cut(s)	3012
MroXI	GAANNNNTTC	1 cut(s)	1609
MscI	TGGCCA	1 cut(s)	1257
MseI	TTAA	9 cut(s)	1088, 1295, 1476, 1515, 1923, 2001, 2175, 2217, 2565
MslI	CAYNNNNRTG	2 cut(s)	129, 1530
Msp20I	TGGCCA	1 cut(s)	1257
MspA1I	CMGCKG	2 cut(s)	2652, 3262
MspI	CCGG	4 cut(s)	105, 1335, 1912, 3013
MspR9I	CCNGG	7 cut(s)	105, 1181, 1336, 1912, 1913, 2468, 3077
MunI	CAATTG	4 cut(s)	1001, 1507, 2589, 3153
Mva1269I	GAATGC	5 cut(s)	85, 146, 700, 2622, 2670
MvaI	CCWGG	3 cut(s)	1181, 2468, 3077
MvnI	CGCG	1 cut(s)	1353
MwoI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2384, 3272, 3545
NarI	GGCGCC	1 cut(s)	3138
NciI	CCSGG	4 cut(s)	105, 1336, 1912, 1913
NcoI	CCATGG	1 cut(s)	2331
NdeI	CATATG	2 cut(s)	1615, 3268
NlaIV	GGNNCC	9 cut(s)	103, 1016, 1291, 1521, 2139, 3011, 3139, 3274, 3282
NmuCI	GTSAC	4 cut(s)	1414, 2545, 2684, 3370
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	1 cut(s)	3126
PaeR7I	CTCGAG	1 cut(s)	1221
PagI	TCATGA	2 cut(s)	640, 832
PciI	ACATGT	1 cut(s)	1191
PctI	GAATGC	5 cut(s)	85, 146, 700, 2622, 2670
PdmI	GAANNNNTTC	1 cut(s)	1609
PflFI	GACNNNGTC	1 cut(s)	1859
PflMI	CCANNNNNTGG	1 cut(s)	1730
PfoI	TCCNGGA	1 cut(s)	2466
PkrI	GCNGC	8 cut(s)	30, 384, 765, 1072, 1642, 1657, 3071, 3403
Ple19I	CGATCG	1 cut(s)	437
PleI	GAGTC	3 cut(s)	641, 729, 773
PluTI	GGCGCC	1 cut(s)	3141
PpsI	GAGTC	3 cut(s)	641, 729, 773
Ppu21I	YACGTR	1 cut(s)	1229
PpuMI	RGGWCCY	1 cut(s)	2470
PscI	ACATGT	1 cut(s)	1191
PshBI	ATTAAT	1 cut(s)	2217
PsiI	TTATAA	1 cut(s)	1755
Psp5II	RGGWCCY	1 cut(s)	2470
Psp6I	CCWGG	3 cut(s)	1179, 2466, 3075
PspEI	GGTNACC	1 cut(s)	970
PspFI	CCCAGC	1 cut(s)	3006
PspGI	CCWGG	3 cut(s)	1179, 2466, 3075
PspN4I	GGNNCC	9 cut(s)	103, 1016, 1291, 1521, 2139, 3011, 3139, 3274, 3282
PspPI	GGNCC	6 cut(s)	101, 367, 505, 2470, 3010, 3280
PspPPI	RGGWCCY	1 cut(s)	2470
PspXI	VCTCGAGB	1 cut(s)	1221
PstI	CTGCAG	2 cut(s)	2113, 2237
PstNI	CAGNNNCTG	3 cut(s)	769, 1186, 2952
PsuI	RGATCY	2 cut(s)	1183, 1748
PsyI	GACNNNGTC	1 cut(s)	1859
PvuI	CGATCG	1 cut(s)	437
PvuII	CAGCTG	1 cut(s)	3262
RseI	CAYNNNNRTG	2 cut(s)	129, 1530
SaqAI	TTAA	9 cut(s)	1088, 1295, 1476, 1515, 1923, 2001, 2175, 2217, 2565
SatI	GCNGC	8 cut(s)	29, 383, 764, 1071, 1641, 1656, 3070, 3402
Sau96I	GGNCC	6 cut(s)	101, 367, 505, 2470, 3010, 3280
ScaI	AGTACT	1 cut(s)	1959
SchI	GAGTC	3 cut(s)	641, 730, 773
ScrFI	CCNGG	7 cut(s)	105, 1181, 1336, 1912, 1913, 2468, 3077
SduI	GDGCHC	6 cut(s)	1161, 1228, 1693, 1920, 2989, 3331
SfaNI	GCATC	5 cut(s)	439, 1710, 1927, 2510, 3081
SfcI	CTRYAG	4 cut(s)	1035, 1550, 2109, 2233
SfoI	GGCGCC	1 cut(s)	3139
Sfr274I	CTCGAG	1 cut(s)	1221
SfuI	TTCGAA	1 cut(s)	3126
SinI	GGWCC	4 cut(s)	505, 2470, 3010, 3280
SlaI	CTCGAG	1 cut(s)	1221
SmaI	CCCGGG	1 cut(s)	1913
SmiMI	CAYNNNNRTG	2 cut(s)	129, 1530
SmlI	CTYRAG	4 cut(s)	146, 1221, 1786, 2957
SmoI	CTYRAG	4 cut(s)	146, 1221, 1786, 2957
SsiI	CCGC	6 cut(s)	293, 382, 1353, 2652, 3402, 3415
SspDI	GGCGCC	1 cut(s)	3137
SspI	AATATT	1 cut(s)	2221
SspMI	CTAG	4 cut(s)	114, 978, 2393, 2480
StyD4I	CCNGG	7 cut(s)	103, 1179, 1334, 1910, 1911, 2466, 3075
StyI	CCWWGG	2 cut(s)	2331, 3276
TaaI	ACNGT	9 cut(s)	59, 282, 1039, 1109, 1554, 1859, 2703, 3176, 3526
TaiI	ACGT	1 cut(s)	1231
TaqI	TCGA	8 cut(s)	433, 1139, 1222, 2044, 2058, 2245, 3126, 3509
TaqII	GACCGA	1 cut(s)	2508
TatI	WGTACW	5 cut(s)	705, 1104, 1194, 1957, 3147
TauI	GCSGC	2 cut(s)	385, 3404
Tru1I	TTAA	9 cut(s)	1088, 1295, 1476, 1515, 1923, 2001, 2175, 2217, 2565
Tru9I	TTAA	9 cut(s)	1088, 1295, 1476, 1515, 1923, 2001, 2175, 2217, 2565
TscAI	CASTG	6 cut(s)	160, 231, 802, 1390, 1864, 3304
TseFI	GTSAC	4 cut(s)	1414, 2545, 2684, 3370
TseI	GCWGC	6 cut(s)	28, 763, 1070, 1640, 1655, 3069
Tsp45I	GTSAC	4 cut(s)	1414, 2545, 2684, 3370
TspGWI	ACGGA	1 cut(s)	61
TspMI	CCCGGG	1 cut(s)	1911
TspRI	CASTG	6 cut(s)	160, 231, 802, 1390, 1864, 3304
Tth111I	GACNNNGTC	1 cut(s)	1859
Van91I	CCANNNNNTGG	1 cut(s)	1730
VneI	GTGCAC	2 cut(s)	2985, 3327
VpaK11BI	GGWCC	4 cut(s)	505, 2470, 3010, 3280
VspI	ATTAAT	1 cut(s)	2217
XapI	RAATTY	4 cut(s)	446, 1676, 1997, 2434
XbaI	TCTAGA	2 cut(s)	113, 2479
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2329, 3266
XhoI	CTCGAG	1 cut(s)	1221
XmaI	CCCGGG	1 cut(s)	1911
XmiI	GTMKAC	2 cut(s)	197, 912
XmnI	GAANNNNTTC	1 cut(s)	1609
XspI	CTAG	4 cut(s)	114, 978, 2393, 2480
ZrmI	AGTACT	1 cut(s)	1959

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.