Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Reverse (-)

30193204 .. 30197734

Gene Structure 4531 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1219500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2853 bp

ATGGAGATTGAAGATCAACCGTACCCTCTTACGCCACAGCAGCTTCTTCTTCGTGACTCCAAGCTGCGAAACACTGATTTTATATATGGGGTGGTAATCTTTACAGGTCACGATACAAAAGTTATGCAGAATTCAACAGATCCTCCTTCTAAGAGAAGCAAAGTTGAGAAGCGGATGGATAAAATTATCTACTTCTTGTTTTTCCTCTTAGTTCTGATGTCTTTTGTTGGGGCAATTGTCTTCGGGGTTAATACTAGTAAAGACCTGGATGATGGGAAATTGATCAGATGGTACCTCAGACCAGATGATACCACAGTTTACTATGATCCGACAAGGGCACCGCTTGCAGCAGTTTTGCAATTTTTAACTGCCATTATGCTGTATAGTTATTTGATCCCCATTTCCTTGTATGTGTCAATAGAAATTGTCAAAGTTCTTCAATGCACTTTCATAAACCAGGATGTGCACATGTACTATGAGGAAACTGACAAGCCAGCCCGAGCGCTTACTTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATACTTTCAGATAAAACCGGAACATTAACTTGCAACTCAATGGAATTTATCAAGTGTTCTATTGCTGGCACTGCTTACGGGCGTGGAGTTACTGAAGTTGAGAGAGCTTTAGCTAGGAGAAAAGGCGAGGAGTTGACAGAAGAAGAGATCCATGCTGAAGAATTGAGTGAAGCCACATCGATAAAAGGCTTTAATTTTATGGATGAAAGGATCATGAATGGAAAATGGGTTAATGAGCCTCGCGCAGATGTAATCCAGAAGTTTCTGCAGTTACTTGCAATCTGTCATACTGCAATACCTGAAGTTGATGAAGAAACCGGAATTGTATCATATGAAGCTGAATCACCAGATGAGGCCGCTTTTGTGATTGCAGCAAGAGAGTTTGGGTTTGAATTTTATGAAAGGAGTCAAGGAAGCATCTCATTGCATGAGTTAGACCCTATTTACGGAAGAAAAATTGAAAGAACTTATAAACTTTTGAACATATTGGAGTTTAGTAGTTCAAGAAAGCGGATGTCCGTAATTGTAAGAAATGAAGAGGGAAGGATACTACTGCTATGCAAAGGCGCTGACAGTGTCATGTTTGAAAGACTTGCAAATAACGGAAGGGAGTTTGAAGAGAAGACTAGGGAACACATTAACGAGTATGCTGATGCTGGTTTGAGGACTTTGGTACTTGCATATCGCGAACTTGACGAAGAAGAATATGATGAGTTCAACAAAGAATTCACTGAGGCCAAAACGTTGGTGAGTTCAGATCAGGAGGAAATTGTCGAGGAAGTTTCAGAGAAGATCGAGAGGGATTTGATTCTTCTCGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCCGAATGCATTGACAAGCTTGCTCAGGCGGGAATTAAAATATGGGTTTTGACTGGAGATAAAATGGAGACAGCGATTAATATTGGATATGCGTGCAGTTTGCTTAGACAAGGAATGAAACGAATAGTAATAAGCTCTGAGACTGCAGAAGCTAAAGCATTAGAGAAGGTGGAGGATAAGACGGCAGTAACCAAGGCATTGAAGGAAAGCGTAGTCCATCAAATAAGTCAGGGAAAGGCCTTGCTTGCTGCACCAGAAGAGAACTCTGAGGCATTGGCTTTGATCATTGATGGGAATTCCCTCGCTTATGCCTTAGAGAACGATGTCAAGGACCTCTTTCTAGAGCTTGCCATCGGCTGCGCATCTGTTATATGCTGTCGTTCATCTCCCAAGCAGAAAGCACTTGTTACAAGGCTGGTAAAAGATAAAACTGGCAACACAACTCTGGCCATTGGTGATGGGGCAAATGATGTTGGAATGCTTCAAGAAGCAGATATCGGAGTTGGTATCAGTGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGCGACATTGCAATTGCACAGTTCCGATTTTTGGAGCGCCTGCTACTTGTACATGGCCATTGGTGTTACAGAAGAATCTCATCAATGATATGCTATTTCTTCTACAAGAACATTGCTTTTGGCTTCACCATTTTCTTCTATCAGATCTATGCTTCATTCTCCGGCCAAACTGCGTACAATGATTGGTATCTGTCACTATACAATGTCTTCTTCACGTCACTTCCGGTGATTGCCTTGGGAGTTTTTGACCAAGATGTCTCGGCCAAGTTATGTCTCAAGTTCCCGCTATTGTACCAAGAAGGTACACAAAATGTCTTATTTAGCTGGCTCCGGATACTTGGATGGGCAATGAATGGGGTGGTGACTGCTACCCTAGTCTTCTTCTTCTGCCTTGTGGCAATGGGCAGTCAAGCCTTTCGTAAAGGCGGGCAAGTGGTTGGCTTGGAAATCTTTGGTGCGACGATGTACTCATGTGTGGTATGGGTCGTCAACTGTCAGATGGCGCTGTCCATCAACTACTTCACTTACATACAACATCTCTTCATCTGGGGTGGCATTGTGTTATGGTATATATTCCAGTTGGGATATGGAGCCCTTGACCCCGACATATCGACCACTGCATATAAGGTGTTCATTGAAGCCTGCGCACCGGCTCCGTTTTATTGGCTTCTCACACTGTTTGTTTTGATCTCTTCCCTCCTGCCATACTTCATTTACGCAGCCATCCAAATGCGGTTTTTCCCAATGTTTCACCAGATGATACAGTGGATAAGAACAGACGGGCAATCAGATGACCCCGAGTTCTGTCAAATGGTGCGGCAGAGATCCCTAAGAAGCACAACAGTAGGTTACACAGCCCGCATCGAAGCAACATCTAGACGCTTTGGAGAGAAGCTCGAGGTTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

951

Amino Acids

107.52

Weight (kDa)

5.01

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase	PF13246	268 - 464	3.7e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	646 - 896	1.4e-86	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1017
AasI	GACNNNNNNGTC	1 cut(s)	2198
Acc16I	TGCGCA	2 cut(s)	1762, 2590
Acc65I	GGTACC	1 cut(s)	291
AccB1I	GGYRCC	2 cut(s)	291, 337
AccII	CGCG	2 cut(s)	789, 1233
AccIII	TCCGGA	1 cut(s)	2274
AclI	AACGTT	1 cut(s)	1289
AclWI	GGATC	6 cut(s)	134, 320, 388, 688, 764, 2763
AcoI	YGGCCR	5 cut(s)	532, 1848, 1999, 2107, 2205
AcsI	RAATTY	5 cut(s)	130, 590, 938, 1271, 1696
AcuI	CTGAAG	3 cut(s)	660, 723, 867
AfaI	GTAC	9 cut(s)	23, 293, 473, 1221, 1995, 2120, 2237, 2248, 2411
AfeI	AGCGCT	1 cut(s)	504
AfiI	CCNNNNNNNGG	2 cut(s)	992, 1975
AflIII	ACRYGT	1 cut(s)	468
AhlI	ACTAGT	1 cut(s)	254
AjiI	CACGTC	1 cut(s)	2160
AjnI	CCWGG	2 cut(s)	264, 456
Alw21I	GWGCWC	1 cut(s)	468
Alw26I	GTCTC	4 cut(s)	1463, 1535, 2206, 2222
Alw44I	GTGCAC	1 cut(s)	464
AlwI	GGATC	6 cut(s)	134, 320, 388, 688, 764, 2763
Ama87I	CYCGRG	3 cut(s)	498, 2741, 2840
Aor13HI	TCCGGA	1 cut(s)	2274
Aor51HI	AGCGCT	1 cut(s)	504
AoxI	GGCC	8 cut(s)	532, 900, 1281, 1638, 1848, 1999, 2107, 2205
ApaLI	GTGCAC	1 cut(s)	464
ApeKI	GCWGC	7 cut(s)	40, 64, 347, 917, 1649, 1758, 2663
ApoI	RAATTY	5 cut(s)	130, 590, 938, 1271, 1696
AseI	ATTAAT	1 cut(s)	1479
Asp718I	GGTACC	1 cut(s)	291
AspLEI	GCGC	7 cut(s)	505, 791, 1115, 1763, 1983, 2449, 2591
AspS9I	GGNCC	1 cut(s)	1732
AsuHPI	GGTGA	7 cut(s)	882, 1306, 1868, 2062, 2182, 2317, 2687
AvaI	CYCGRG	3 cut(s)	498, 2741, 2840
AvaII	GGWCC	1 cut(s)	1732
BaeGI	GKGCMC	2 cut(s)	340, 468
BaeI	ACNNNNGTAYC	4 cut(s)	105, 138, 300, 333
BalI	TGGCCA	3 cut(s)	534, 1850, 2001
BanI	GGYRCC	2 cut(s)	291, 337
BanII	GRGCYC	1 cut(s)	2539
BbsI	GAAGAC	5 cut(s)	232, 1175, 1386, 2143, 2314
Bbv12I	GWGCWC	1 cut(s)	468
BbvI	GCAGC	7 cut(s)	51, 52, 359, 929, 1636, 1745, 2675
BceAI	ACGGC	1 cut(s)	1599
BciT130I	CCWGG	2 cut(s)	266, 458
BciVI	GTATCC	2 cut(s)	1086, 2271
BclI	TGATCA	2 cut(s)	282, 1683
BcoDI	GTCTC	4 cut(s)	1463, 1535, 2206, 2222
BcuI	ACTAGT	1 cut(s)	254
BfaI	CTAG	6 cut(s)	255, 660, 1173, 1742, 2318, 2820
BfmI	CTRYAG	3 cut(s)	812, 1371, 1545
BfoI	RGCGCY	4 cut(s)	506, 1116, 1984, 2450
BfuI	GTATCC	2 cut(s)	1086, 2271
BglII	AGATCT	1 cut(s)	2088
BisI	GCNGC	9 cut(s)	41, 65, 348, 903, 918, 1650, 1759, 2664, 2762
BlsI	GCNGC	9 cut(s)	42, 66, 349, 904, 919, 1651, 1760, 2665, 2763
Bme1390I	CCNGG	2 cut(s)	266, 458
Bme18I	GGWCC	1 cut(s)	1732
BmeT110I	CYCGRG	3 cut(s)	498, 2741, 2840
BmgBI	CACGTC	1 cut(s)	2160
BmgT120I	GGNCC	1 cut(s)	1732
BmiI	GGNNCC	6 cut(s)	293, 339, 1401, 2273, 2536, 2598
BmrFI	CCNGG	2 cut(s)	266, 458
BmsI	GCATC	4 cut(s)	972, 1189, 1772, 2814
BpiI	GAAGAC	5 cut(s)	232, 1175, 1386, 2143, 2314
BplI	GAGNNNNNCTC	1 cut(s)	2823
BpmI	CTGGAG	1 cut(s)	1476
Bpu10I	CCTNAGC	1 cut(s)	1425
BpuEI	CTTGAG	1 cut(s)	2204
Bsa29I	ATCGAT	1 cut(s)	725
BsaBI	GATNNNNATC	1 cut(s)	2130
BsaJI	CCNNGG	2 cut(s)	1593, 2178
BsaWI	WCCGGW	4 cut(s)	563, 863, 2167, 2274
BsaXI	ACNNNNNCTCC	2 cut(s)	127, 157
Bsc4I	CCNNNNNNNGG	2 cut(s)	992, 1975
Bse118I	RCCGGY	1 cut(s)	2593
Bse1I	ACTGG	3 cut(s)	1459, 1837, 2521
Bse3DI	GCAATG	5 cut(s)	968, 1950, 2055, 2298, 2349
Bse8I	GATNNNNATC	1 cut(s)	2130
BseAI	TCCGGA	1 cut(s)	2274
BseBI	CCWGG	2 cut(s)	266, 458
BseCI	ATCGAT	1 cut(s)	725
BseDI	CCNNGG	2 cut(s)	1593, 2178
BseGI	GGATG	7 cut(s)	180, 274, 466, 754, 1065, 2291, 2667
BseJI	GATNNNNATC	1 cut(s)	2130
BseLI	CCNNNNNNNGG	2 cut(s)	992, 1975
BseMI	GCAATG	5 cut(s)	968, 1950, 2055, 2298, 2349
BseMII	CTCAG	5 cut(s)	310, 1269, 1439, 1530, 1659
BseNI	ACTGG	3 cut(s)	1459, 1837, 2521
BseRI	GAGGAG	1 cut(s)	689
BseSI	GKGCMC	2 cut(s)	340, 468
BseXI	GCAGC	7 cut(s)	51, 52, 359, 929, 1636, 1745, 2675
BsgI	GTGCAG	2 cut(s)	1516, 1635
Bsh1236I	CGCG	2 cut(s)	789, 1233
BshFI	GGCC	8 cut(s)	534, 902, 1283, 1640, 1850, 2001, 2109, 2207
BshNI	GGYRCC	2 cut(s)	291, 337
BshVI	ATCGAT	1 cut(s)	725
BsiHKAI	GWGCWC	1 cut(s)	468
BsiHKCI	CYCGRG	3 cut(s)	498, 2741, 2840
BsiSI	CCGG	6 cut(s)	564, 864, 2106, 2168, 2275, 2594
BslI	CCNNNNNNNGG	2 cut(s)	992, 1975
BsmAI	GTCTC	4 cut(s)	1463, 1535, 2206, 2222
BsmI	GAATGC	3 cut(s)	1412, 1884, 1932
BsnI	GGCC	8 cut(s)	534, 902, 1283, 1640, 1850, 2001, 2109, 2207
BsoBI	CYCGRG	3 cut(s)	498, 2741, 2840
Bsp1286I	GDGCHC	3 cut(s)	340, 468, 2539
Bsp13I	TCCGGA	1 cut(s)	2274
Bsp1407I	TGTACA	1 cut(s)	1993
Bsp68I	TCGCGA	1 cut(s)	1233
BspANI	GGCC	8 cut(s)	534, 902, 1283, 1640, 1850, 2001, 2109, 2207
BspCNI	CTCAG	5 cut(s)	309, 1270, 1438, 1531, 1660
BspDI	ATCGAT	1 cut(s)	725
BspEI	TCCGGA	1 cut(s)	2274
BspFNI	CGCG	2 cut(s)	789, 1233
BspHI	TCATGA	1 cut(s)	759
BspLI	GGNNCC	6 cut(s)	293, 339, 1401, 2273, 2536, 2598
BspMAI	CTGCAG	3 cut(s)	816, 1375, 1549
BspPI	GGATC	6 cut(s)	134, 320, 388, 688, 764, 2763
BspT107I	GGYRCC	2 cut(s)	291, 337
BsrDI	GCAATG	5 cut(s)	968, 1950, 2055, 2298, 2349
BsrFI	RCCGGY	1 cut(s)	2593
BsrGI	TGTACA	1 cut(s)	1993
BsrI	ACTGG	3 cut(s)	1459, 1837, 2521
BssAI	RCCGGY	1 cut(s)	2593
BssECI	CCNNGG	2 cut(s)	1593, 2178
BssT1I	CCWWGG	2 cut(s)	1593, 2178
Bst2UI	CCWGG	2 cut(s)	266, 458
Bst4CI	ACNGT	8 cut(s)	21, 316, 1121, 1965, 2438, 2622, 2709, 2788
Bst6I	CTCTTC	6 cut(s)	684, 1077, 1158, 1653, 2489, 2641
BstAPI	GCANNNNNTGC	1 cut(s)	344
BstAUI	TGTACA	1 cut(s)	1993
BstF5I	GGATG	7 cut(s)	180, 274, 466, 754, 1065, 2291, 2667
BstFNI	CGCG	2 cut(s)	789, 1233
BstH2I	RGCGCY	4 cut(s)	506, 1116, 1984, 2450
BstHHI	GCGC	7 cut(s)	505, 791, 1115, 1763, 1983, 2449, 2591
BstMAI	GTCTC	4 cut(s)	1463, 1535, 2206, 2222
BstMWI	GCNNNNNNNGC	4 cut(s)	40, 344, 617, 1646
BstNI	CCWGG	2 cut(s)	266, 458
BstNSI	RCATGY	1 cut(s)	472
BstSCI	CCNGG	2 cut(s)	264, 456
BstSFI	CTRYAG	3 cut(s)	812, 1371, 1545
BstSLI	GKGCMC	2 cut(s)	340, 468
BstUI	CGCG	2 cut(s)	789, 1233
BstV1I	GCAGC	7 cut(s)	51, 52, 359, 929, 1636, 1745, 2675
BstV2I	GAAGAC	5 cut(s)	232, 1175, 1386, 2143, 2314
BstX2I	RGATCY	4 cut(s)	139, 693, 2088, 2768
BstXI	CCANNNNNNTGG	1 cut(s)	1291
BstYI	RGATCY	4 cut(s)	139, 693, 2088, 2768
Bsu15I	ATCGAT	1 cut(s)	725
BsuI	GTATCC	2 cut(s)	1086, 2271
BsuRI	GGCC	8 cut(s)	534, 902, 1283, 1640, 1850, 2001, 2109, 2207
BsuTUI	ATCGAT	1 cut(s)	725
BtrI	CACGTC	1 cut(s)	2160
BtsCI	GGATG	7 cut(s)	180, 274, 466, 754, 1065, 2291, 2667
BtsI	GCAGTG	2 cut(s)	615, 2559
BtsIMutI	CAGTG	8 cut(s)	72, 615, 1126, 1275, 1918, 2559, 2618, 2714
BtuMI	TCGCGA	1 cut(s)	1233
CciI	TCATGA	1 cut(s)	759
CfoI	GCGC	7 cut(s)	505, 791, 1115, 1763, 1983, 2449, 2591
Cfr10I	RCCGGY	1 cut(s)	2593
Cfr13I	GGNCC	1 cut(s)	1732
ClaI	ATCGAT	1 cut(s)	725
CseI	GACGC	1 cut(s)	2832
Csp6I	GTAC	9 cut(s)	22, 292, 472, 1220, 1994, 2119, 2236, 2247, 2410
CviAII	CATG	8 cut(s)	469, 698, 760, 974, 1126, 1939, 1997, 2415
CviQI	GTAC	9 cut(s)	22, 292, 472, 1220, 1994, 2119, 2236, 2247, 2410
DrdI	GACNNNNNNGTC	1 cut(s)	2198
DseDI	GACNNNNNNGTC	1 cut(s)	2198
EaeI	YGGCCR	5 cut(s)	532, 1848, 1999, 2107, 2205
Eam1104I	CTCTTC	6 cut(s)	684, 1077, 1158, 1653, 2489, 2641
EarI	CTCTTC	6 cut(s)	684, 1077, 1158, 1653, 2489, 2641
Eco130I	CCWWGG	2 cut(s)	1593, 2178
Eco147I	AGGCCT	1 cut(s)	1640
Eco24I	GRGCYC	1 cut(s)	2539
Eco32I	GATATC	1 cut(s)	1897
Eco47I	GGWCC	1 cut(s)	1732
Eco47III	AGCGCT	1 cut(s)	504
Eco57I	CTGAAG	3 cut(s)	660, 723, 867
Eco88I	CYCGRG	3 cut(s)	498, 2741, 2840
EcoO109I	RGGNCCY	1 cut(s)	1732
EcoRI	GAATTC	3 cut(s)	130, 1271, 1696
EcoRII	CCWGG	2 cut(s)	264, 456
EcoRV	GATATC	1 cut(s)	1897
EcoT14I	CCWWGG	2 cut(s)	1593, 2178
EcoT22I	ATGCAT	1 cut(s)	1412
EcoT38I	GRGCYC	1 cut(s)	2539
ErhI	CCWWGG	2 cut(s)	1593, 2178
FaeI	CATG	8 cut(s)	472, 701, 763, 977, 1129, 1942, 2000, 2418
FalI	AAGNNNNNCTT	4 cut(s)	1371, 1403, 1721, 1753
FatI	CATG	8 cut(s)	468, 697, 759, 973, 1125, 1938, 1996, 2414
FauI	CCCGC	4 cut(s)	1423, 2235, 2363, 2810
FauNDI	CATATG	1 cut(s)	877
FbaI	TGATCA	2 cut(s)	282, 1683
Fnu4HI	GCNGC	9 cut(s)	41, 65, 348, 903, 918, 1650, 1759, 2664, 2762
FokI	GGATG	7 cut(s)	187, 281, 473, 761, 1072, 2298, 2654
FriOI	GRGCYC	1 cut(s)	2539
Fsp4HI	GCNGC	9 cut(s)	41, 65, 348, 903, 918, 1650, 1759, 2664, 2762
FspBI	CTAG	6 cut(s)	255, 660, 1173, 1742, 2318, 2820
FspI	TGCGCA	2 cut(s)	1762, 2590
GlaI	GCGC	7 cut(s)	504, 790, 1114, 1762, 1982, 2448, 2590
GluI	GCNGC	9 cut(s)	41, 65, 348, 903, 918, 1650, 1759, 2664, 2762
GsuI	CTGGAG	1 cut(s)	1476
HaeII	RGCGCY	4 cut(s)	506, 1116, 1984, 2450
HaeIII	GGCC	8 cut(s)	534, 902, 1283, 1640, 1850, 2001, 2109, 2207
HapII	CCGG	6 cut(s)	564, 864, 2106, 2168, 2275, 2594
HgaI	GACGC	1 cut(s)	2832
HhaI	GCGC	7 cut(s)	505, 791, 1115, 1763, 1983, 2449, 2591
Hin1II	CATG	8 cut(s)	472, 701, 763, 977, 1129, 1942, 2000, 2418
Hin6I	GCGC	7 cut(s)	503, 789, 1113, 1761, 1981, 2447, 2589
HinP1I	GCGC	7 cut(s)	503, 789, 1113, 1761, 1981, 2447, 2589
HincII	GTYRAC	2 cut(s)	681, 2434
HindII	GTYRAC	2 cut(s)	681, 2434
HindIII	AAGCTT	1 cut(s)	1418
HinfI	GANTC	5 cut(s)	56, 887, 952, 1354, 2019
HpaII	CCGG	6 cut(s)	564, 864, 2106, 2168, 2275, 2594
HphI	GGTGA	7 cut(s)	882, 1306, 1868, 2062, 2182, 2317, 2687
Hpy166II	GTNNAC	5 cut(s)	319, 466, 681, 2249, 2434
Hpy8I	GTNNAC	5 cut(s)	319, 466, 681, 2249, 2434
Hpy99I	CGWCG	1 cut(s)	2407
HpyAV	CCTTC	7 cut(s)	156, 1083, 1146, 1561, 1597, 1916, 2237
HpyCH4III	ACNGT	8 cut(s)	21, 316, 1121, 1965, 2438, 2622, 2709, 2788
HpyCH4IV	ACGT	2 cut(s)	1289, 2159
HpyF10VI	GCNNNNNNNGC	4 cut(s)	40, 344, 617, 1646
HpySE526I	ACGT	2 cut(s)	1289, 2159
Hsp92II	CATG	8 cut(s)	472, 701, 763, 977, 1129, 1942, 2000, 2418
HspAI	GCGC	7 cut(s)	503, 789, 1113, 1761, 1981, 2447, 2589
Kpn2I	TCCGGA	1 cut(s)	2274
KpnI	GGTACC	1 cut(s)	295
Ksp22I	TGATCA	2 cut(s)	282, 1683
LmnI	GCTCC	4 cut(s)	1978, 2277, 2534, 2602
Lsp1109I	GCAGC	7 cut(s)	51, 52, 359, 929, 1636, 1745, 2675
LweI	GCATC	4 cut(s)	972, 1189, 1772, 2814
MaeI	CTAG	6 cut(s)	255, 660, 1173, 1742, 2318, 2820
MaeII	ACGT	2 cut(s)	1289, 2159
MfeI	CAATTG	2 cut(s)	234, 1956
MflI	RGATCY	4 cut(s)	139, 693, 2088, 2768
MhlI	GDGCHC	3 cut(s)	340, 468, 2539
MlsI	TGGCCA	3 cut(s)	534, 1850, 2001
MluNI	TGGCCA	3 cut(s)	534, 1850, 2001
MlyI	GAGTC	2 cut(s)	50, 961
MmeI	TCCRAC	2 cut(s)	353, 1855
Mox20I	TGGCCA	3 cut(s)	534, 1850, 2001
Mph1103I	ATGCAT	1 cut(s)	1412
MroI	TCCGGA	1 cut(s)	2274
MscI	TGGCCA	3 cut(s)	534, 1850, 2001
MseI	TTAA	9 cut(s)	249, 365, 572, 738, 777, 1185, 1437, 1479, 2851
MslI	CAYNNNNRTG	1 cut(s)	2672
Msp20I	TGGCCA	3 cut(s)	534, 1850, 2001
MspI	CCGG	6 cut(s)	564, 864, 2106, 2168, 2275, 2594
MspR9I	CCNGG	2 cut(s)	266, 458
MunI	CAATTG	2 cut(s)	234, 1956
Mva1269I	GAATGC	3 cut(s)	1412, 1884, 1932
MvaI	CCWGG	2 cut(s)	266, 458
MvnI	CGCG	2 cut(s)	789, 1233
MwoI	GCNNNNNNNGC	4 cut(s)	40, 344, 617, 1646
NdeI	CATATG	1 cut(s)	877
NlaIII	CATG	8 cut(s)	472, 701, 763, 977, 1129, 1942, 2000, 2418
NlaIV	GGNNCC	6 cut(s)	293, 339, 1401, 2273, 2536, 2598
NmeAIII	GCCGAG	1 cut(s)	2183
NmuCI	GTSAC	5 cut(s)	53, 107, 2136, 2160, 2305
NruI	TCGCGA	1 cut(s)	1233
NsbI	TGCGCA	2 cut(s)	1762, 2590
NsiI	ATGCAT	1 cut(s)	1412
NspI	RCATGY	1 cut(s)	472
PaeR7I	CTCGAG	1 cut(s)	2840
PagI	TCATGA	1 cut(s)	759
PceI	AGGCCT	1 cut(s)	1640
PciI	ACATGT	1 cut(s)	468
PctI	GAATGC	3 cut(s)	1412, 1884, 1932
PfeI	GAWTC	3 cut(s)	887, 1354, 2019
PflFI	GACNNNGTC	1 cut(s)	1121
PkrI	GCNGC	9 cut(s)	42, 66, 349, 904, 919, 1651, 1760, 2665, 2763
PleI	GAGTC	2 cut(s)	50, 960
PpsI	GAGTC	2 cut(s)	50, 960
PpuMI	RGGWCCY	1 cut(s)	1732
PscI	ACATGT	1 cut(s)	468
PshBI	ATTAAT	1 cut(s)	1479
PsiI	TTATAA	1 cut(s)	1017
Psp1406I	AACGTT	1 cut(s)	1289
Psp5II	RGGWCCY	1 cut(s)	1732
Psp6I	CCWGG	2 cut(s)	264, 456
PspGI	CCWGG	2 cut(s)	264, 456
PspN4I	GGNNCC	6 cut(s)	293, 339, 1401, 2273, 2536, 2598
PspPI	GGNCC	1 cut(s)	1732
PspPPI	RGGWCCY	1 cut(s)	1732
PspXI	VCTCGAGB	1 cut(s)	2840
PstI	CTGCAG	3 cut(s)	816, 1375, 1549
PsuI	RGATCY	4 cut(s)	139, 693, 2088, 2768
PsyI	GACNNNGTC	1 cut(s)	1121
RruI	TCGCGA	1 cut(s)	1233
RsaI	GTAC	9 cut(s)	23, 293, 473, 1221, 1995, 2120, 2237, 2248, 2411
RsaNI	GTAC	9 cut(s)	22, 292, 472, 1220, 1994, 2119, 2236, 2247, 2410
RseI	CAYNNNNRTG	1 cut(s)	2672
SaqAI	TTAA	9 cut(s)	249, 365, 572, 738, 777, 1185, 1437, 1479, 2851
SatI	GCNGC	9 cut(s)	41, 65, 348, 903, 918, 1650, 1759, 2664, 2762
Sau96I	GGNCC	1 cut(s)	1732
SchI	GAGTC	2 cut(s)	50, 961
ScrFI	CCNGG	2 cut(s)	266, 458
SduI	GDGCHC	3 cut(s)	340, 468, 2539
SfaNI	GCATC	4 cut(s)	972, 1189, 1772, 2814
SfcI	CTRYAG	3 cut(s)	812, 1371, 1545
Sfr274I	CTCGAG	1 cut(s)	2840
SinI	GGWCC	1 cut(s)	1732
SlaI	CTCGAG	1 cut(s)	2840
SmiMI	CAYNNNNRTG	1 cut(s)	2672
SmlI	CTYRAG	2 cut(s)	2219, 2840
SmoI	CTYRAG	2 cut(s)	2219, 2840
SpeI	ACTAGT	1 cut(s)	254
SseBI	AGGCCT	1 cut(s)	1640
SspI	AATATT	1 cut(s)	1483
SspMI	CTAG	6 cut(s)	255, 660, 1173, 1742, 2318, 2820
StuI	AGGCCT	1 cut(s)	1640
StyD4I	CCNGG	2 cut(s)	264, 456
StyI	CCWWGG	2 cut(s)	1593, 2178
TaaI	ACNGT	8 cut(s)	21, 316, 1121, 1965, 2438, 2622, 2709, 2788
TaiI	ACGT	2 cut(s)	1292, 2162
TaqI	TCGA	6 cut(s)	725, 1320, 1341, 2555, 2808, 2841
TatI	WGTACW	3 cut(s)	471, 1993, 2409
TauI	GCSGC	2 cut(s)	905, 2764
TfiI	GAWTC	3 cut(s)	887, 1354, 2019
Tru1I	TTAA	9 cut(s)	249, 365, 572, 738, 777, 1185, 1437, 1479, 2851
Tru9I	TTAA	9 cut(s)	249, 365, 572, 738, 777, 1185, 1437, 1479, 2851
TscAI	CASTG	8 cut(s)	79, 622, 1126, 1282, 1918, 2566, 2625, 2714
TseFI	GTSAC	5 cut(s)	53, 107, 2136, 2160, 2305
TseI	GCWGC	7 cut(s)	40, 64, 347, 917, 1649, 1758, 2663
Tsp45I	GTSAC	5 cut(s)	53, 107, 2136, 2160, 2305
TspGWI	ACGGA	4 cut(s)	1008, 1054, 1164, 2589
TspRI	CASTG	8 cut(s)	79, 622, 1126, 1282, 1918, 2566, 2625, 2714
Tth111I	GACNNNGTC	1 cut(s)	1121
VneI	GTGCAC	1 cut(s)	464
VpaK11BI	GGWCC	1 cut(s)	1732
VspI	ATTAAT	1 cut(s)	1479
XapI	RAATTY	5 cut(s)	130, 590, 938, 1271, 1696
XbaI	TCTAGA	2 cut(s)	1741, 2819
XceI	RCATGY	1 cut(s)	472
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2528
XhoI	CTCGAG	1 cut(s)	2840
XspI	CTAG	6 cut(s)	255, 660, 1173, 1742, 2318, 2820
Zsp2I	ATGCAT	1 cut(s)	1412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.