Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

64217326 .. 64222112

Gene Structure 4787 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004988

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGGGAAAAGAGGCCCTTGAAGATTGGCGGCGCAAAAAGCAGGATATGGATGTGAACAACAGAAAAGTTCTGGTGCATCGATCCGAAGGTGAATTTGATTTTACCAAGTGGAGGGATTTGAAAGTTGGGGATATAGTGAAAGTGGAAAAGGACCAATTTTTCCCTGCTGATCTTATCTTACTTTCATCAAGCTATGATGAAGCACTTTGCTATGTAGAGACCACCAACCTTGATGGGGAAACTAATTTGAAAATAAAACAATCTCTGGAAGCAACTTCTAATCTTCATGAAGACTCGAGCTTCAAAGATTTCAAGGCTTTGATCAGATGTGAAGACCCGAATGCAAGTCTGTACTCATTCGTAGGGAGTCTGGAGTTTGGAGGGGAATCGTACCCTCTTTCACCTCAGCAGCTTCTGCTTAGGGACTCTAAGCTGAGAAACACTGAGTTTATTTTTGGGGTTGTAATCTTCACAGGTCATGATACAAAAGTTATGCAAAACTCAACTGAACCACCTTCCAAGAGAAGCAAAATTGAGAAAAGGATGGATAAGATAGTCTACTTCTTGTTTTTCCTCTTAGTTGTGATGTCTTTTGTTGGTGCTATTGTCTTTGGGGTTACCACTAGCGAAGACATAGAAGATGGGCAATTGATAAGATGGTACCTTAGACCAGATGATACTACAGTTTACTATGATCCAACAAGAGCGCCACTTGCAGCCATTTTGCAGTTCTTAACAGCCGTTATGTTGTACAGTTATTTGATTCCCATTTCCTTGTATGTGTCGATAGAGATTGTCAAAGTACTCCAGAGCATTTTCATCAACCAGGATCTGCACATGTACTACGAGGAAACTGACAAGCCAGCTCGAGCACGTACCTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATTCTATCGGACAAAACAGGAACCTTAACTTGCAACTCAATGGAGTTTATCAAGTGTTCTATTGCCGGGACTGCTTTTGGACGCGGAGTTACAGAAGTTGAGAGAGCTTTGTCCAGTGGCAAAGGCTCATCTTTGTATGAAGGGGTGACAGAAGAAGAAGGCCCAGTTGAAGAATCTACTGAAGCCAAGACATCAATAAAAGGCTTTAACTTCATGGATGAAAGAATAATGGATGGCAATTGGGTTAAGGAGCCTCGTGCAGATGTAATCCAGAAGTTTCTACAGTTACTCGCTGTCTGTCATACAGCAGTACCTGAAGTTGATGAAGAATCTGGAAGAGTTTCATACGAGGCTGAATCACCAGATGAGGCAGCTTTTGTGATTGCAGCAAGAGAGCTTGGTTTTGAATTTTATGAAAGGGCTCAAACAAGCATCTCCCTGCACGAGTTTGATCCAATGTCTGGAAGGAGAGTTGAAAGATCTTATAAGATTTTGAATGTATTGGAGTTTAGTAGCTCAAGGAAGCGAATGTCTGTGATTGTAAGAAATGAGGTGGGAAAGATACTATTGCTCAGTAAAGGTGCTGACAGTGTCATGTTCCAAAGACTTGCAAGGGATGGAAGGGAGTTTGAAGAGAAGACCCGGGAGCACATTAACGAGTATGCTGATGCTGGTTTGAGGACTTTAGTACTTGCATATCGGGAACTTGATGAGGAAGAGTATTTTGAATTTAACAAAGAATATACTGAGGCCAAGAACTTGGTTAGTTCAGATCGAGAGGAAGCTGTCGAGGAAGTGGCTGAGAAGATAGAGAGGAACTTGATTCTTCTTGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCCGACTGCATTGACAAGCTTGCTCAGGCTGGAATTAAACTATGGGTTTTGACTGGAGATAAAATGGAGACAGCAATTAATATTGGATATGCCTGCAGTTTGCTTCGACAAGGAATGAAACAAATAATCATAAGCTCAGAGACCCCAGAAGCTAAAGCGCTAGAGAAGGTGGAGGACAAGTCCAAAGTTGCCATGGCATTGAAGGAAAGTGTTCTCCATCAAATAAGTGAGGCAAGGGCATTTCTTGCTTCACCAGATCAGAACTCCGAAGCATTGGCTTTGATCATTGATGGGAATTCACTCGCTTATGCCTTAGAGGTAGATGTCCAGGACCTCTTTCTAGAGCTTGCCATTGGCTGTGCATCTGTTATATGCTGTCGTTCATCTCCCAAACAGAAAGCACTTGTCACAAGACTGGTAAAAGTTAAAACTGGTAAAACAACTCTGGCAATTGGTGATGGGGCAAATGATGTAGGAATGCTTCAAGAAGCAGATATTGGTGTTGGTATCAGCGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGTGACATTGCAATTGCACAGTTTCGTTTTCTGGAGCGCCTGCTACTCGTACATGGACATTGGTGTTACAGAAGAATCTCGTCAATGATATGCTATTTCTTTTACAAGAACATTACTTTTGGCTTCACTATCTTTTTCTATGAGATGTACGCTTCATTCTCGGGTCAATCTGCATATAATGATTGGTATCTATCACTCTATAACGTCTTCTTCACATCGCTTCCTGTAATTGCATTGGGAGTGTTTGACCAAGATGTCTCAGCCAGATTCTGCCTCAAGTTCCCACTCTTGTACCAAGAAGGTGCACAAAATGTCTTATTTAGCTGGGTCCGGATACTAGGATGGTCGGTGAACGGCATAGTGTCTGCTATGATAATCTTCTTCTGCTGCATCCTGGCAATAGGTAGTCAAGCCTTTCGCAAAGGCGGGGAAGTTGTTGGCTTCGAAATCTTTGGTGCCACAATGTACTCAATTGTGGTATGGGTTGTCAACTGTCAGATGGCACTATCCATCAGTTACTTCACTTACATACAACATCTCTTCATCTGGGGTGGCATTGTGTTCTGGTACATATTCCAGCTGGCATATGGAGCCTTGGACCCTTCCATATCAACCACTGCCTATAAGGTCTTCATTGAAGCCTGTGCACCAGCCCCATTTTATTGGCTTCTCACACTCTTGGTAGTAGTCACTTCTCTCCTACCTTATTTCACTTACGCCGCCTTCCAAATGCGATTCTTCCCCATGTATCATCAGATGATACAATGGATGAGAACAGATGGGCAATCAGATGACCCTGAGTTCTGTCATATGGTGAGGCAGAGATCGCTAAGACCCACAACAGTAGGCTACACAGCTCGCATTGCGACATCTAAACGCTTTGAAGAGAAGCCCGAGAGTAGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1079

Amino Acids

121.98

Weight (kDa)

5.06

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase	PF13246	396 - 592	5.6e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	774 - 1024	4.8e-87	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1401
AasI	GACNNNNNNGTC	1 cut(s)	2582
Acc65I	GGTACC	1 cut(s)	660
AccB1I	GGYRCC	2 cut(s)	660, 2783
AccB7I	CCANNNNNTGG	1 cut(s)	1376
AccI	GTMKAC	1 cut(s)	558
AccII	CGCG	1 cut(s)	999
AccIII	TCCGGA	1 cut(s)	2658
AciI	CCGC	5 cut(s)	28, 999, 2298, 2754, 3048
AclWI	GGATC	4 cut(s)	75, 689, 837, 1361
AcoI	YGGCCR	1 cut(s)	901
AcsI	RAATTY	4 cut(s)	92, 1322, 1643, 2080
AcuI	CTGAAG	2 cut(s)	1116, 1251
AfeI	AGCGCT	1 cut(s)	1944
AfiI	CCNNNNNNNGG	3 cut(s)	111, 235, 1376
AflIII	ACRYGT	1 cut(s)	837
AjnI	CCWGG	3 cut(s)	825, 2112, 2721
AjuI	GAANNNNNNNTTGG	2 cut(s)	2265, 2297
Alw21I	GWGCWC	4 cut(s)	874, 1566, 2635, 2977
Alw26I	GTCTC	4 cut(s)	212, 1847, 1919, 2590
Alw44I	GTGCAC	2 cut(s)	2631, 2973
AlwI	GGATC	4 cut(s)	75, 689, 837, 1361
AlwNI	CAGNNNCTG	4 cut(s)	415, 832, 1229, 2598
Ama87I	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
Aor13HI	TCCGGA	1 cut(s)	2658
Aor51HI	AGCGCT	1 cut(s)	1944
AoxI	GGCC	4 cut(s)	12, 901, 1075, 1665
ApaLI	GTGCAC	2 cut(s)	2631, 2973
ApeKI	GCWGC	5 cut(s)	409, 716, 1286, 1301, 2715
ApoI	RAATTY	4 cut(s)	92, 1322, 1643, 2080
AseI	ATTAAT	1 cut(s)	1863
Asp700I	GAANNNNTTC	2 cut(s)	1255, 1995
Asp718I	GGTACC	1 cut(s)	660
AspLEI	GCGC	4 cut(s)	33, 709, 1945, 2367
AspS9I	GGNCC	6 cut(s)	13, 151, 1076, 2116, 2656, 2926
AsuC2I	CCSGG	3 cut(s)	982, 1558, 1559
AsuHPI	GGTGA	8 cut(s)	101, 393, 1072, 1266, 2028, 2252, 2689, 3154
AsuII	TTCGAA	1 cut(s)	2772
AvaI	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
AvaII	GGWCC	4 cut(s)	151, 2116, 2656, 2926
BaeGI	GKGCMC	2 cut(s)	2635, 2977
BaeI	ACNNNNGTAYC	8 cut(s)	474, 507, 669, 702, 1209, 1242, 2603, 2636
BalI	TGGCCA	1 cut(s)	903
BanI	GGYRCC	2 cut(s)	660, 2783
BanII	GRGCYC	1 cut(s)	1339
BarI	GAAGNNNNNNTAC	2 cut(s)	2250, 2282
BauI	CACGAG	2 cut(s)	1170, 1358
BbsI	GAAGAC	7 cut(s)	297, 339, 636, 1559, 1770, 2527, 2950
Bbv12I	GWGCWC	4 cut(s)	874, 1566, 2635, 2977
BbvCI	CCTCAGC	1 cut(s)	405
BbvI	GCAGC	5 cut(s)	421, 728, 1298, 1313, 2702
BceAI	ACGGC	2 cut(s)	725, 2698
BciT130I	CCWGG	3 cut(s)	827, 2114, 2723
BciVI	GTATCC	1 cut(s)	2655
BclI	TGATCA	2 cut(s)	321, 2067
BcnI	CCSGG	3 cut(s)	982, 1558, 1559
BcoDI	GTCTC	4 cut(s)	212, 1847, 1919, 2590
BfaI	CTAG	4 cut(s)	624, 1946, 2126, 2666
BfmI	CTRYAG	4 cut(s)	681, 1196, 1755, 1879
BfoI	RGCGCY	3 cut(s)	710, 1946, 2368
BfuI	GTATCC	1 cut(s)	2655
BglII	AGATCT	1 cut(s)	1394
BisI	GCNGC	7 cut(s)	29, 410, 717, 1287, 1302, 2716, 3048
BlsI	GCNGC	7 cut(s)	30, 411, 718, 1288, 1303, 2717, 3049
BmcAI	AGTACT	2 cut(s)	804, 1605
Bme1390I	CCNGG	6 cut(s)	827, 982, 1558, 1559, 2114, 2723
Bme18I	GGWCC	4 cut(s)	151, 2116, 2656, 2926
BmeT110I	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
BmgT120I	GGNCC	6 cut(s)	13, 151, 1076, 2116, 2656, 2926
BmiI	GGNNCC	8 cut(s)	662, 937, 1167, 1785, 2657, 2785, 2920, 2928
BmrFI	CCNGG	6 cut(s)	827, 982, 1558, 1559, 2114, 2723
BmrI	ACTGGG	1 cut(s)	1073
BmsI	GCATC	5 cut(s)	85, 1356, 1573, 2156, 2727
BmuI	ACTGGG	1 cut(s)	1073
BpiI	GAAGAC	7 cut(s)	297, 339, 636, 1559, 1770, 2527, 2950
BpmI	CTGGAG	4 cut(s)	392, 791, 1860, 2381
Bpu10I	CCTNAGC	3 cut(s)	405, 419, 1809
Bpu14I	TTCGAA	1 cut(s)	2772
BpuEI	CTTGAG	2 cut(s)	1417, 2588
BpuMI	CCSGG	3 cut(s)	982, 1558, 1559
Bsa29I	ATCGAT	1 cut(s)	79
BsaAI	YACGTR	1 cut(s)	875
BsaBI	GATNNNNATC	1 cut(s)	2514
BsaI	GGTCTC	2 cut(s)	212, 1919
BsaJI	CCNNGG	3 cut(s)	1557, 1977, 2922
BsaWI	WCCGGW	1 cut(s)	2658
BsaXI	ACNNNNNCTCC	2 cut(s)	1949, 1979
Bsc4I	CCNNNNNNNGG	3 cut(s)	111, 235, 1376
Bse1I	ACTGG	5 cut(s)	1029, 1079, 1843, 2205, 2221
Bse3DI	GCAATG	2 cut(s)	2334, 3189
Bse8I	GATNNNNATC	1 cut(s)	2514
BseAI	TCCGGA	1 cut(s)	2658
BseBI	CCWGG	3 cut(s)	827, 2114, 2723
BseCI	ATCGAT	1 cut(s)	79
BseDI	CCNNGG	3 cut(s)	1557, 1977, 2922
BseGI	GGATG	8 cut(s)	55, 549, 1138, 1153, 1537, 2675, 2718, 3102
BseJI	GATNNNNATC	1 cut(s)	2514
BseLI	CCNNNNNNNGG	3 cut(s)	111, 235, 1376
BseMI	GCAATG	2 cut(s)	2334, 3189
BseNI	ACTGG	5 cut(s)	1029, 1079, 1843, 2205, 2221
BseSI	GKGCMC	2 cut(s)	2635, 2977
BseXI	GCAGC	5 cut(s)	421, 728, 1298, 1313, 2702
BseYI	CCCAGC	1 cut(s)	2652
BsgI	GTGCAG	3 cut(s)	818, 1194, 1340
Bsh1236I	CGCG	1 cut(s)	999
BshFI	GGCC	4 cut(s)	14, 903, 1077, 1667
BshNI	GGYRCC	2 cut(s)	660, 2783
BshVI	ATCGAT	1 cut(s)	79
BsiHKAI	GWGCWC	4 cut(s)	874, 1566, 2635, 2977
BsiHKCI	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
BsiSI	CCGG	3 cut(s)	981, 1558, 2659
BslFI	GGGAC	2 cut(s)	437, 997
BslI	CCNNNNNNNGG	3 cut(s)	111, 235, 1376
BsmAI	GTCTC	4 cut(s)	212, 1847, 1919, 2590
BsmFI	GGGAC	2 cut(s)	437, 997
BsmI	GAATGC	3 cut(s)	346, 2268, 2316
BsnI	GGCC	4 cut(s)	14, 903, 1077, 1667
Bso31I	GGTCTC	2 cut(s)	212, 1919
BsoBI	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
Bsp119I	TTCGAA	1 cut(s)	2772
Bsp1286I	GDGCHC	5 cut(s)	874, 1339, 1566, 2635, 2977
Bsp13I	TCCGGA	1 cut(s)	2658
Bsp1407I	TGTACA	1 cut(s)	750
Bsp19I	CCATGG	1 cut(s)	1977
BspACI	CCGC	5 cut(s)	28, 999, 2298, 2754, 3048
BspANI	GGCC	4 cut(s)	14, 903, 1077, 1667
BspDI	ATCGAT	1 cut(s)	79
BspEI	TCCGGA	1 cut(s)	2658
BspFNI	CGCG	1 cut(s)	999
BspHI	TCATGA	2 cut(s)	286, 478
BspLI	GGNNCC	8 cut(s)	662, 937, 1167, 1785, 2657, 2785, 2920, 2928
BspMAI	CTGCAG	2 cut(s)	1759, 1883
BspPI	GGATC	4 cut(s)	75, 689, 837, 1361
BspT104I	TTCGAA	1 cut(s)	2772
BspT107I	GGYRCC	2 cut(s)	660, 2783
BspTNI	GGTCTC	2 cut(s)	212, 1919
BsrDI	GCAATG	2 cut(s)	2334, 3189
BsrGI	TGTACA	1 cut(s)	750
BsrI	ACTGG	5 cut(s)	1029, 1079, 1843, 2205, 2221
BssECI	CCNNGG	3 cut(s)	1557, 1977, 2922
BssSI	CACGAG	2 cut(s)	1170, 1358
BssT1I	CCWWGG	2 cut(s)	1977, 2922
Bst2BI	CACGAG	2 cut(s)	1170, 1358
Bst2UI	CCWGG	3 cut(s)	827, 2114, 2723
Bst4CI	ACNGT	7 cut(s)	685, 755, 1200, 1505, 2349, 2822, 3172
Bst6I	CTCTTC	5 cut(s)	1246, 1542, 1626, 2873, 3207
BstAPI	GCANNNNNTGC	2 cut(s)	415, 2030
BstAUI	TGTACA	1 cut(s)	750
BstBAI	YACGTR	1 cut(s)	875
BstBI	TTCGAA	1 cut(s)	2772
BstC8I	GCNNGC	8 cut(s)	864, 1806, 1879, 2133, 2316, 2369, 2910, 3187
BstDSI	CCRYGG	1 cut(s)	1977
BstEII	GGTNACC	1 cut(s)	616
BstF5I	GGATG	8 cut(s)	55, 549, 1138, 1153, 1537, 2675, 2718, 3102
BstFNI	CGCG	1 cut(s)	999
BstH2I	RGCGCY	3 cut(s)	710, 1946, 2368
BstHHI	GCGC	4 cut(s)	33, 709, 1945, 2367
BstMAI	GTCTC	4 cut(s)	212, 1847, 1919, 2590
BstMWI	GCNNNNNNNGC	8 cut(s)	37, 415, 713, 986, 2030, 2918, 3154, 3191
BstNI	CCWGG	3 cut(s)	827, 2114, 2723
BstNSI	RCATGY	1 cut(s)	841
BstPI	GGTNACC	1 cut(s)	616
BstSCI	CCNGG	6 cut(s)	825, 980, 1556, 1557, 2112, 2721
BstSFI	CTRYAG	4 cut(s)	681, 1196, 1755, 1879
BstSLI	GKGCMC	2 cut(s)	2635, 2977
BstUI	CGCG	1 cut(s)	999
BstV1I	GCAGC	5 cut(s)	421, 728, 1298, 1313, 2702
BstV2I	GAAGAC	7 cut(s)	297, 339, 636, 1559, 1770, 2527, 2950
BstX2I	RGATCY	2 cut(s)	829, 1394
BstXI	CCANNNNNNTGG	2 cut(s)	1675, 2991
BstYI	RGATCY	2 cut(s)	829, 1394
Bsu15I	ATCGAT	1 cut(s)	79
BsuI	GTATCC	1 cut(s)	2655
BsuRI	GGCC	4 cut(s)	14, 903, 1077, 1667
BsuTUI	ATCGAT	1 cut(s)	79
BtgI	CCRYGG	1 cut(s)	1977
BtgZI	GCGATG	1 cut(s)	2529
BtsCI	GGATG	8 cut(s)	55, 549, 1138, 1153, 1537, 2675, 2718, 3102
BtsI	GCAGTG	1 cut(s)	2943
BtsIMutI	CAGTG	4 cut(s)	441, 1036, 1510, 2943
Cac8I	GCNNGC	8 cut(s)	864, 1806, 1879, 2133, 2316, 2369, 2910, 3187
CaiI	CAGNNNCTG	4 cut(s)	415, 832, 1229, 2598
CciI	TCATGA	2 cut(s)	286, 478
CfoI	GCGC	4 cut(s)	33, 709, 1945, 2367
Cfr13I	GGNCC	6 cut(s)	13, 151, 1076, 2116, 2656, 2926
Cfr9I	CCCGGG	1 cut(s)	1557
ClaI	ATCGAT	1 cut(s)	79
CseI	GACGC	1 cut(s)	1005
CspCI	CAANNNNNGTGG	2 cut(s)	211, 246
CviAII	CATG	9 cut(s)	287, 479, 838, 1129, 1510, 1978, 2323, 2381, 3073
DrdI	GACNNNNNNGTC	1 cut(s)	2582
DseDI	GACNNNNNNGTC	1 cut(s)	2582
EaeI	YGGCCR	1 cut(s)	901
Eam1104I	CTCTTC	5 cut(s)	1246, 1542, 1626, 2873, 3207
EarI	CTCTTC	5 cut(s)	1246, 1542, 1626, 2873, 3207
Eco130I	CCWWGG	2 cut(s)	1977, 2922
Eco24I	GRGCYC	1 cut(s)	1339
Eco31I	GGTCTC	2 cut(s)	212, 1919
Eco47I	GGWCC	4 cut(s)	151, 2116, 2656, 2926
Eco47III	AGCGCT	1 cut(s)	1944
Eco57I	CTGAAG	2 cut(s)	1116, 1251
Eco88I	CYCGRG	5 cut(s)	295, 867, 1557, 2488, 3221
Eco91I	GGTNACC	1 cut(s)	616
EcoO109I	RGGNCCY	2 cut(s)	13, 2116
EcoO65I	GGTNACC	1 cut(s)	616
EcoRI	GAATTC	1 cut(s)	2080
EcoRII	CCWGG	3 cut(s)	825, 2112, 2721
EcoT14I	CCWWGG	2 cut(s)	1977, 2922
EcoT38I	GRGCYC	1 cut(s)	1339
ErhI	CCWWGG	2 cut(s)	1977, 2922
FaeI	CATG	9 cut(s)	290, 482, 841, 1132, 1513, 1981, 2326, 2384, 3076
FalI	AAGNNNNNCTT	3 cut(s)	32, 1755, 1787
FaqI	GGGAC	2 cut(s)	437, 997
FatI	CATG	9 cut(s)	286, 478, 837, 1128, 1509, 1977, 2322, 2380, 3072
FauI	CCCGC	1 cut(s)	2747
FauNDI	CATATG	2 cut(s)	2914, 3138
FbaI	TGATCA	2 cut(s)	321, 2067
FblI	GTMKAC	1 cut(s)	558
Fnu4HI	GCNGC	7 cut(s)	29, 410, 717, 1287, 1302, 2716, 3048
FokI	GGATG	8 cut(s)	62, 556, 1145, 1160, 1544, 2682, 2705, 3109
FriOI	GRGCYC	1 cut(s)	1339
Fsp4HI	GCNGC	7 cut(s)	29, 410, 717, 1287, 1302, 2716, 3048
FspBI	CTAG	4 cut(s)	624, 1946, 2126, 2666
GlaI	GCGC	4 cut(s)	32, 708, 1944, 2366
GluI	GCNGC	7 cut(s)	29, 410, 717, 1287, 1302, 2716, 3048
GsaI	CCCAGC	1 cut(s)	2656
GsuI	CTGGAG	4 cut(s)	392, 791, 1860, 2381
HaeII	RGCGCY	3 cut(s)	710, 1946, 2368
HaeIII	GGCC	4 cut(s)	14, 903, 1077, 1667
HapII	CCGG	3 cut(s)	981, 1558, 2659
HgaI	GACGC	1 cut(s)	1005
HhaI	GCGC	4 cut(s)	33, 709, 1945, 2367
Hin1II	CATG	9 cut(s)	290, 482, 841, 1132, 1513, 1981, 2326, 2384, 3076
Hin6I	GCGC	4 cut(s)	31, 707, 1943, 2365
HinP1I	GCGC	4 cut(s)	31, 707, 1943, 2365
HincII	GTYRAC	1 cut(s)	2818
HindII	GTYRAC	1 cut(s)	2818
HindIII	AAGCTT	1 cut(s)	1802
HpaII	CCGG	3 cut(s)	981, 1558, 2659
HphI	GGTGA	8 cut(s)	101, 393, 1072, 1266, 2028, 2252, 2689, 3154
Hpy166II	GTNNAC	7 cut(s)	55, 559, 688, 2633, 2680, 2818, 2975
Hpy8I	GTNNAC	7 cut(s)	55, 559, 688, 2633, 2680, 2818, 2975
HpyCH4III	ACNGT	7 cut(s)	685, 755, 1200, 1505, 2349, 2822, 3172
HpyCH4IV	ACGT	2 cut(s)	874, 2532
HpyF10VI	GCNNNNNNNGC	8 cut(s)	37, 415, 713, 986, 2030, 2918, 3154, 3191
HpySE526I	ACGT	2 cut(s)	874, 2532
Hsp92II	CATG	9 cut(s)	290, 482, 841, 1132, 1513, 1981, 2326, 2384, 3076
HspAI	GCGC	4 cut(s)	31, 707, 1943, 2365
Kpn2I	TCCGGA	1 cut(s)	2658
KpnI	GGTACC	1 cut(s)	664
Ksp22I	TGATCA	2 cut(s)	321, 2067
LmnI	GCTCC	4 cut(s)	1165, 1561, 2362, 2918
Lsp1109I	GCAGC	5 cut(s)	421, 728, 1298, 1313, 2702
LweI	GCATC	5 cut(s)	85, 1356, 1573, 2156, 2727
MaeI	CTAG	4 cut(s)	624, 1946, 2126, 2666
MaeII	ACGT	2 cut(s)	874, 2532
MaeIII	GTNAC	9 cut(s)	616, 1003, 1060, 1200, 2191, 2330, 2393, 2843, 3016
MfeI	CAATTG	5 cut(s)	647, 1153, 2235, 2340, 2799
MflI	RGATCY	2 cut(s)	829, 1394
MhlI	GDGCHC	5 cut(s)	874, 1339, 1566, 2635, 2977
MlsI	TGGCCA	1 cut(s)	903
MluNI	TGGCCA	1 cut(s)	903
MlyI	GAGTC	3 cut(s)	287, 376, 419
MmeI	TCCRAC	1 cut(s)	722
Mox20I	TGGCCA	1 cut(s)	903
MroI	TCCGGA	1 cut(s)	2658
MroXI	GAANNNNTTC	2 cut(s)	1255, 1995
MscI	TGGCCA	1 cut(s)	903
MseI	TTAA	9 cut(s)	734, 941, 1122, 1161, 1569, 1647, 1821, 1863, 2211
Msp20I	TGGCCA	1 cut(s)	903
MspA1I	CMGCKG	2 cut(s)	2298, 2908
MspI	CCGG	3 cut(s)	981, 1558, 2659
MspR9I	CCNGG	6 cut(s)	827, 982, 1558, 1559, 2114, 2723
MunI	CAATTG	5 cut(s)	647, 1153, 2235, 2340, 2799
Mva1269I	GAATGC	3 cut(s)	346, 2268, 2316
MvaI	CCWGG	3 cut(s)	827, 2114, 2723
MvnI	CGCG	1 cut(s)	999
MwoI	GCNNNNNNNGC	8 cut(s)	37, 415, 713, 986, 2030, 2918, 3154, 3191
NciI	CCSGG	3 cut(s)	982, 1558, 1559
NcoI	CCATGG	1 cut(s)	1977
NdeI	CATATG	2 cut(s)	2914, 3138
NlaIII	CATG	9 cut(s)	290, 482, 841, 1132, 1513, 1981, 2326, 2384, 3076
NlaIV	GGNNCC	8 cut(s)	662, 937, 1167, 1785, 2657, 2785, 2920, 2928
NmuCI	GTSAC	4 cut(s)	1060, 2191, 2330, 3016
NspI	RCATGY	1 cut(s)	841
NspV	TTCGAA	1 cut(s)	2772
PaeR7I	CTCGAG	2 cut(s)	295, 867
PagI	TCATGA	2 cut(s)	286, 478
PciI	ACATGT	1 cut(s)	837
PctI	GAATGC	3 cut(s)	346, 2268, 2316
PdmI	GAANNNNTTC	2 cut(s)	1255, 1995
PfeI	GAWTC	9 cut(s)	386, 763, 1088, 1244, 1271, 1738, 2403, 2595, 3063
PflFI	GACNNNGTC	1 cut(s)	1505
PflMI	CCANNNNNTGG	1 cut(s)	1376
PfoI	TCCNGGA	1 cut(s)	2112
PkrI	GCNGC	7 cut(s)	30, 411, 718, 1288, 1303, 2717, 3049
PleI	GAGTC	3 cut(s)	287, 375, 419
PpsI	GAGTC	3 cut(s)	287, 375, 419
Ppu21I	YACGTR	1 cut(s)	875
PpuMI	RGGWCCY	1 cut(s)	2116
PscI	ACATGT	1 cut(s)	837
PshBI	ATTAAT	1 cut(s)	1863
PsiI	TTATAA	1 cut(s)	1401
Psp5II	RGGWCCY	1 cut(s)	2116
Psp6I	CCWGG	3 cut(s)	825, 2112, 2721
PspEI	GGTNACC	1 cut(s)	616
PspFI	CCCAGC	1 cut(s)	2652
PspGI	CCWGG	3 cut(s)	825, 2112, 2721
PspN4I	GGNNCC	8 cut(s)	662, 937, 1167, 1785, 2657, 2785, 2920, 2928
PspPI	GGNCC	6 cut(s)	13, 151, 1076, 2116, 2656, 2926
PspPPI	RGGWCCY	1 cut(s)	2116
PspXI	VCTCGAGB	2 cut(s)	295, 867
PstI	CTGCAG	2 cut(s)	1759, 1883
PstNI	CAGNNNCTG	4 cut(s)	415, 832, 1229, 2598
PsuI	RGATCY	2 cut(s)	829, 1394
PsyI	GACNNNGTC	1 cut(s)	1505
PvuII	CAGCTG	1 cut(s)	2908
SaqAI	TTAA	9 cut(s)	734, 941, 1122, 1161, 1569, 1647, 1821, 1863, 2211
SatI	GCNGC	7 cut(s)	29, 410, 717, 1287, 1302, 2716, 3048
Sau96I	GGNCC	6 cut(s)	13, 151, 1076, 2116, 2656, 2926
ScaI	AGTACT	2 cut(s)	804, 1605
SchI	GAGTC	3 cut(s)	287, 376, 419
ScrFI	CCNGG	6 cut(s)	827, 982, 1558, 1559, 2114, 2723
SduI	GDGCHC	5 cut(s)	874, 1339, 1566, 2635, 2977
SfaNI	GCATC	5 cut(s)	85, 1356, 1573, 2156, 2727
SfcI	CTRYAG	4 cut(s)	681, 1196, 1755, 1879
Sfr274I	CTCGAG	2 cut(s)	295, 867
SfuI	TTCGAA	1 cut(s)	2772
SinI	GGWCC	4 cut(s)	151, 2116, 2656, 2926
SlaI	CTCGAG	2 cut(s)	295, 867
SmaI	CCCGGG	1 cut(s)	1559
SmlI	CTYRAG	4 cut(s)	295, 867, 1432, 2603
SmoI	CTYRAG	4 cut(s)	295, 867, 1432, 2603
SsiI	CCGC	5 cut(s)	28, 999, 2298, 2754, 3048
SspI	AATATT	1 cut(s)	1867
SspMI	CTAG	4 cut(s)	624, 1946, 2126, 2666
StyD4I	CCNGG	6 cut(s)	825, 980, 1556, 1557, 2112, 2721
StyI	CCWWGG	2 cut(s)	1977, 2922
TaaI	ACNGT	7 cut(s)	685, 755, 1200, 1505, 2349, 2822, 3172
TaiI	ACGT	2 cut(s)	877, 2535
TaqI	TCGA	8 cut(s)	79, 296, 785, 868, 1690, 1704, 1891, 2772
TatI	WGTACW	6 cut(s)	351, 750, 802, 840, 1603, 2793
TauI	GCSGC	2 cut(s)	31, 3050
TfiI	GAWTC	9 cut(s)	386, 763, 1088, 1244, 1271, 1738, 2403, 2595, 3063
Tru1I	TTAA	9 cut(s)	734, 941, 1122, 1161, 1569, 1647, 1821, 1863, 2211
Tru9I	TTAA	9 cut(s)	734, 941, 1122, 1161, 1569, 1647, 1821, 1863, 2211
TscAI	CASTG	4 cut(s)	448, 1036, 1510, 2950
TseFI	GTSAC	4 cut(s)	1060, 2191, 2330, 3016
TseI	GCWGC	5 cut(s)	409, 716, 1286, 1301, 2715
Tsp45I	GTSAC	4 cut(s)	1060, 2191, 2330, 3016
TspMI	CCCGGG	1 cut(s)	1557
TspRI	CASTG	4 cut(s)	448, 1036, 1510, 2950
Tth111I	GACNNNGTC	1 cut(s)	1505
Van91I	CCANNNNNTGG	1 cut(s)	1376
VneI	GTGCAC	2 cut(s)	2631, 2973
VpaK11BI	GGWCC	4 cut(s)	151, 2116, 2656, 2926
VspI	ATTAAT	1 cut(s)	1863
XapI	RAATTY	4 cut(s)	92, 1322, 1643, 2080
XbaI	TCTAGA	1 cut(s)	2125
XceI	RCATGY	1 cut(s)	841
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1975, 2912
XhoI	CTCGAG	2 cut(s)	295, 867
XmaI	CCCGGG	1 cut(s)	1557
XmiI	GTMKAC	1 cut(s)	558
XmnI	GAANNNNTTC	2 cut(s)	1255, 1995
XspI	CTAG	4 cut(s)	624, 1946, 2126, 2666
ZrmI	AGTACT	2 cut(s)	804, 1605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.