Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

3581239 .. 3586779

Gene Structure 5541 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1050400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3591 bp

ATGAGGGGTGGAGGCAGAAGGAGAAAGCTGCATTTCAGCAAGATCTATTCTTTCACTTGTGGTAAATCTTCTATGAGAGATGAACATTCACAAATCGGGGGACCAGGATTTTCCAGAGTGGTTTACTGCAACGAACCGGAATCTTTCGAGGCTCAGATGCAAAATTACGGAGACAATTATGTTAGAAGTACAAAGTATACACTCGCTACTTTCTTGCCAAAATCTTTGTTTGAGCAGTTCAGAAGGGTGGCCAACTTCTACTTCTTAGTCACTGGCATTTTGTCCTTCACAGCTCTGGCTCCATATAGCGCTGTCAGCGCGATCATCCCTCTTATTATTGTGATTAGCGCGACCATGGTGAAAGAGGGTATCGAAGATTGGCATCGAAAACAACAGGATATTGAGGTGAACAATCGAAAGGTTAAAGTACGTGATGGTAATGGGGCTTTCAATTATACTGCGTGGAGAAATCTGAGAGTGGGAGATATAGTGAAGGTAGAAAAGGACGAATTCTTTCCGACAGACCTCCTCTTGCTCTCATCCAGTTTTGACGATGCAATTTGCTATGTTGAGACCATGAACCTTGATGGGGAGACAAATTTAAAGTTAAAACAAGGGTTGGAAGTAACTTTATCCTTGCGGGAGGATTCTAACTTCAACGATTTTAACGCCATTGTTAAATGTGAAGACCCGAATGCAAATTTGTACTCATTTGTTGGAACTATGGAGTTCGACAATGAACAATTTCCCGTCTCTCCTCAACAGCTTCTCCTGAGAGACTCTAAACTCAGAAATACAGACTACGTATATGGAGCTGTTATCTTCACTGGTCCTGACACAAAGGTTATTCAAAATTCTACCGCCCCTCCTTCAAAAAGAAGCAGAGTTGAGAAGAAGATGGATAAAATTATCTACTTCTTGTTCTGTGTTATATTTTTAATGGCATTTGTTGGGTCCATTTTCTTTGGGATTGCAACGAAAGATGACCTAAACAAAGGGATCATGAAAAGGTGGTATCTCAGGCCAGATAATTCTAGAATTTTCTTTGATGCTAAAAGAGCTCCGTATGCAGCAATTTACCACTTTTTGACTGCCCTAATGTTGTATGGCAACTTTATCCCCATCTCCTTGTATGTGTCCATAGAAATTGTCAAAGTTCTTCAGAGCATCTTCATCAATCGAGATGTTCACATGTACTATGAGGAGGCTGACAAACCAGCACACGCCCGTACCTCAAACTTGAACGAGGAACTTGGCCAAGTCGACACAATTCTCTCTGATAAGACGGGAACTTTGACCTGCAATTCAATGGAGTTCATTAAGTGTTCTGTGGCCGGGACAGCTTATGGCCGCGGCTTTACTGAAGTTGAGAGGGCTATGGGTCGACGAAATGGTTCACCATTGGTTCATCAACTAAGTGGAGGGGACAATCTCAAGGACTCTACTGAAACAAAGGCACCCATCAAAGGCTTTAATTTTAAAGACAAAAGGGTCATGAATGGAAACTGGGTTAATGAGCCTAATGCAGAATACATCCAGAAGTTTTTCAGTTTATTAGCAATCTGTCATACAGCAATTCCGGAAGTTGATAAAGCTACTGGGAATGTTTCATATGAGGCTGAATCTCCTGATGAAGCAGCATTTGTGATTGCAGCAAGAGAACTTGGTTTTGAATTCTACAAAAGAACGCAAACAACCATATCACTGCGAGAGTTGGATCCAGTGTCTGGCAAGAAAGTTGAGAGGACGTATAGCCTTCTCAATGTTTTAGAGTTTAACAGCACAAGGAAGCGAATGTCTGTGATTGTCAGAAGTGAGGAAGGAAAAATATTATTACTTTCCAAAGGTGCCGACAATGTTATGCTTGAAAGGCTTGCCAAAAATGGTTCGGGTTTTGAAGAAGAGACCATGGATCATCTAAATGAGTATGCTGATGCAGGGCTGAGGACCTTGATACTTGCCTATCGTGTACTTGAGGAAGACGAATACAAAGAGTTCAATCAAAATTTTATCAAGGCCAAGAATTCAATTAGTGCAGATCGTGAAACAGTGATCGATGAAGTAACAGAAAAGATTGAGAGAGATCTAATTCTTCTTGGTGCAACTGCTGTTGAGGACAAACTACAAAATGGGGTTCCTGACTGCATTGACAAGCTTGCCCAAGCAGGAATTAAGATTTGGGTTTTGACTGGAGATAAGATGGAGACTGCCATCAATATTGGGTTTGCATGTAGCTTGCTTAGACAAGGAATGAAGCAAATTGTAATCACTTTGGAGTCTCCGGAGATTAAAGCGTTGGAAAAGGCAGGAGAAAAGGAGGCCATCGCCAAGGCATCAAAAGGAAGTGTTCTCGATCAGATAAATAGGGGAAAGGCTCAGCTTATAGCATCGAGCGGGAACTCTGAGGCATTTGCATTGATCATTGATGGAAAATCGCTTGCTTACGCTTTGGAGGATGATACAAAGAATTTGTTTTTAAATCTTGCAATTGGCTGTGCATCTGTTATTTGCTGCCGTTCATCACCTAAACAGAAGGCACTGGTCACTAGACTGGTAAAATCTGGAACTGGGAAAACAACACTAGCAATCGGTGATGGAGCCAATGACGTGGGCATGCTCCAAGAAGCAGATATCGGGGTTGGAATTAGTGGTGTTGAAGGAATGCAGGCGGTCATGTCAAGTGATATTGCGATTGCACAATTTCGATACTTGGAGCGTCTCCTGCTTGTGCACGGGCATTGGTGTTACAGGAGGATCTCATCAATGATTTGCTACTTCTTCTACAAGAACATCGCATTTGGTTTCACTCTCTTCTTATATGAGGCAAACACATCCTTCTCTGGACAGCCTGCATACAACGACTGGTTTTTGTCTCTTTATAACGTATTCTTCTCATCACTTCCGGTGGTTGCTATGGGAGTTCTCGACCAGGATGTATCTGCGCGGTTTTATCTCAAGTTTCCTCTATTATACCAAGAAGGGGTGCAAAATGTTCTCTTCAGCTGGCGTAGAATATTCGGATGGATGTTGAATGGGTTTACAACTGCCGTGATTATTTTCTTCTTCTGCACGGAAGCGCTAAATCAGCAGGCCTTCAACAATGAGGGCAAAACTGCCGGAAGGGATATTCTCGGGGCAACGATGTACACGTGCACAGTTTGGGTTGTGAACTTGCAGATGGCACTTGCCATCAGTTACTTCACCTTAATACAGCACCTCTTCATCTGGGGTTCAATTGCACTATGGTACCTCTTCCTCTTGGCGTACGGCGCCATGTCACCCTCAGTCTCCACCACTGCCTACAAAATCTTCATTGAAGCCCTGGCCCCCGCCCCTTCGTTCTGGCTCCTCACAATCTTCGTGCCAATCGCCGCTCTAATCCCATACTTCACATGCTCAGCCGTTCAAATGCGGTTCTTCCCAATGTACCATAGAATGATACAGTGGATACGCTACGAGGGCACATCAAATGATCCCGAATTTTGTGACATGGTGCGGCAGAGATCTTTGCGGCCGCAGACTGTTGGTTTCACGGCGCGATTAGCAGCGAGGGCCAACCGTGTAAAAGACAGACATCGTAACCGTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1197

Amino Acids

134.9

Weight (kDa)

8.05

Isoelectric Point (pI)

35.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 108	2.1e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	517 - 709	5.3e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	891 - 1141	5e-88	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2880
AasI	GACNNNNNNGTC	1 cut(s)	1490
Acc36I	ACCTGC	1 cut(s)	1307
Acc65I	GGTACC	1 cut(s)	3246
AccB1I	GGYRCC	4 cut(s)	1456, 1847, 3246, 3269
AccB7I	CCANNNNNTGG	1 cut(s)	1727
AccBSI	CCGCTC	2 cut(s)	2394, 3374
AccI	GTMKAC	3 cut(s)	197, 1263, 1384
AccII	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
AccIII	TCCGGA	2 cut(s)	1579, 2281
AclWI	GGATC	6 cut(s)	1007, 1712, 1725, 1920, 2762, 3467
AcoI	YGGCCR	5 cut(s)	249, 1255, 1332, 1348, 3512
AcuI	CTGAAG	3 cut(s)	1145, 1383, 2983
AcvI	CACGTG	1 cut(s)	3150
AcyI	GRCGYC	1 cut(s)	3270
AfeI	AGCGCT	2 cut(s)	310, 3078
AfiI	CCNNNNNNNGG	4 cut(s)	589, 1466, 1727, 2980
AflIII	ACRYGT	2 cut(s)	1191, 3147
AjiI	CACGTC	1 cut(s)	2608
AjnI	CCWGG	3 cut(s)	103, 2928, 3321
AjuI	GAANNNNNNNTTGG	4 cut(s)	245, 277, 2161, 2193
Alw21I	GWGCWC	3 cut(s)	1063, 2733, 3155
Alw44I	GTGCAC	2 cut(s)	2729, 3151
AlwI	GGATC	6 cut(s)	1007, 1712, 1725, 1920, 2762, 3467
AlwNI	CAGNNNCTG	1 cut(s)	1727
Ama87I	CYCGRG	1 cut(s)	3131
Aor13HI	TCCGGA	2 cut(s)	1579, 2281
Aor51HI	AGCGCT	2 cut(s)	310, 3078
ApaLI	GTGCAC	2 cut(s)	2729, 3151
ApeKI	GCWGC	6 cut(s)	28, 1070, 1637, 1652, 2511, 3545
ArsI	GACNNNNNNTTYG	2 cut(s)	1276, 1308
Asp700I	GAANNNNTTC	4 cut(s)	513, 744, 1393, 1544
Asp718I	GGTACC	1 cut(s)	3246
AspLEI	GCGC	7 cut(s)	311, 320, 350, 2944, 3079, 3272, 3538
AspS9I	GGNCC	6 cut(s)	101, 830, 954, 1947, 3325, 3552
AsuC2I	CCSGG	1 cut(s)	1336
AsuHPI	GGTGA	7 cut(s)	370, 418, 1389, 2514, 2603, 3193, 3270
AvaI	CYCGRG	1 cut(s)	3131
AvaII	GGWCC	4 cut(s)	101, 830, 954, 1947
BaeGI	GKGCMC	3 cut(s)	2733, 3155, 3464
BaeI	ACNNNNGTAYC	4 cut(s)	1213, 1246, 3230, 3263
BalI	TGGCCA	2 cut(s)	251, 1257
BamHI	GGATCC	1 cut(s)	1717
BanI	GGYRCC	4 cut(s)	1456, 1847, 3246, 3269
BanII	GRGCYC	1 cut(s)	1063
BbrPI	CACGTG	1 cut(s)	3150
BbsI	GAAGAC	2 cut(s)	693, 1986
Bbv12I	GWGCWC	3 cut(s)	1063, 2733, 3155
BbvCI	CCTCAGC	1 cut(s)	1943
BbvI	GCAGC	6 cut(s)	15, 1082, 1649, 1664, 2498, 3557
BceAI	ACGGC	5 cut(s)	2499, 3032, 3283, 3386, 3549
BciT130I	CCWGG	3 cut(s)	105, 2930, 3323
BciVI	GTATCC	1 cut(s)	3441
BclI	TGATCA	1 cut(s)	2418
BcnI	CCSGG	1 cut(s)	1336
BfaI	CTAG	3 cut(s)	1035, 2547, 2582
BfoI	RGCGCY	3 cut(s)	312, 3080, 3273
BfuAI	ACCTGC	1 cut(s)	1307
BfuI	GTATCC	1 cut(s)	3441
BglII	AGATCT	3 cut(s)	42, 2083, 3503
BlpI	GCTNAGC	2 cut(s)	2376, 3397
Bme1390I	CCNGG	4 cut(s)	105, 1336, 2930, 3323
Bme18I	GGWCC	4 cut(s)	101, 830, 954, 1947
BmeT110I	CYCGRG	1 cut(s)	3131
BmgBI	CACGTC	1 cut(s)	2608
BmgT120I	GGNCC	6 cut(s)	101, 830, 954, 1947, 3325, 3552
BmrFI	CCNGG	4 cut(s)	105, 1336, 2930, 3323
BmrI	ACTGGG	3 cut(s)	1516, 1608, 2577
BmsI	GCATC	9 cut(s)	147, 391, 544, 1039, 1176, 1924, 2342, 2396, 2507
BmuI	ACTGGG	3 cut(s)	1516, 1608, 2577
BpiI	GAAGAC	2 cut(s)	693, 1986
BpmI	CTGGAG	1 cut(s)	2211
Bpu10I	CCTNAGC	1 cut(s)	1943
Bpu1102I	GCTNAGC	2 cut(s)	2376, 3397
BpuEI	CTTGAG	3 cut(s)	1418, 1994, 2939
BpuMI	CCSGG	1 cut(s)	1336
Bsa29I	ATCGAT	1 cut(s)	2055
BsaAI	YACGTR	3 cut(s)	431, 805, 3150
BsaBI	GATNNNNATC	1 cut(s)	381
BsaHI	GRCGYC	1 cut(s)	3270
BsaI	GGTCTC	2 cut(s)	566, 1898
BsaJI	CCNNGG	5 cut(s)	354, 1351, 1908, 2328, 3321
BsaWI	WCCGGW	4 cut(s)	136, 1579, 2281, 2902
BsaXI	ACNNNNNCTCC	6 cut(s)	474, 504, 753, 783, 850, 880
Bsc4I	CCNNNNNNNGG	4 cut(s)	589, 1466, 1727, 2980
Bse8I	GATNNNNATC	1 cut(s)	381
BseAI	TCCGGA	2 cut(s)	1579, 2281
BseBI	CCWGG	3 cut(s)	105, 2930, 3323
BseCI	ATCGAT	1 cut(s)	2055
BseDI	CCNNGG	5 cut(s)	354, 1351, 1908, 2328, 3321
BseGI	GGATG	8 cut(s)	324, 539, 1533, 2461, 2831, 2938, 3026, 3030
BseJI	GATNNNNATC	1 cut(s)	381
BseLI	CCNNNNNNNGG	4 cut(s)	589, 1466, 1727, 2980
BseRI	GAGGAG	4 cut(s)	518, 747, 1217, 3338
BseSI	GKGCMC	3 cut(s)	2733, 3155, 3464
BseX3I	CGGCCG	1 cut(s)	3512
BseXI	GCAGC	6 cut(s)	15, 1082, 1649, 1664, 2498, 3557
BsgI	GTGCAG	2 cut(s)	2055, 3052
Bsh1236I	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
Bsh1285I	CGRYCG	1 cut(s)	3515
BshNI	GGYRCC	4 cut(s)	1456, 1847, 3246, 3269
BshVI	ATCGAT	1 cut(s)	2055
BsiEI	CGRYCG	1 cut(s)	3515
BsiHKAI	GWGCWC	3 cut(s)	1063, 2733, 3155
BsiHKCI	CYCGRG	1 cut(s)	3131
BsiSI	CCGG	6 cut(s)	137, 1335, 1580, 2282, 2903, 3117
BsiWI	CGTACG	1 cut(s)	3264
BslFI	GGGAC	3 cut(s)	114, 1351, 1439
BslI	CCNNNNNNNGG	4 cut(s)	589, 1466, 1727, 2980
BsmBI	CGTCTC	2 cut(s)	757, 2723
BsmFI	GGGAC	3 cut(s)	114, 1351, 1439
BsmI	GAATGC	2 cut(s)	700, 2667
Bso31I	GGTCTC	2 cut(s)	566, 1898
BsoBI	CYCGRG	1 cut(s)	3131
Bsp1286I	GDGCHC	4 cut(s)	1063, 2733, 3155, 3464
Bsp13I	TCCGGA	2 cut(s)	1579, 2281
Bsp1407I	TGTACA	1 cut(s)	3144
Bsp1720I	GCTNAGC	2 cut(s)	2376, 3397
Bsp19I	CCATGG	2 cut(s)	354, 1908
BspDI	ATCGAT	1 cut(s)	2055
BspEI	TCCGGA	2 cut(s)	1579, 2281
BspFNI	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
BspHI	TCATGA	2 cut(s)	1002, 1494
BspMI	ACCTGC	1 cut(s)	1307
BspPI	GGATC	6 cut(s)	1007, 1712, 1725, 1920, 2762, 3467
BspT107I	GGYRCC	4 cut(s)	1456, 1847, 3246, 3269
BspTNI	GGTCTC	2 cut(s)	566, 1898
BsrBI	CCGCTC	2 cut(s)	2394, 3374
BsrGI	TGTACA	1 cut(s)	3144
BssECI	CCNNGG	5 cut(s)	354, 1351, 1908, 2328, 3321
BssNAI	GTATAC	1 cut(s)	198
BssNI	GRCGYC	1 cut(s)	3270
BssT1I	CCWWGG	3 cut(s)	354, 1908, 2328
Bst1107I	GTATAC	1 cut(s)	198
Bst2UI	CCWGG	3 cut(s)	105, 2930, 3323
Bst4CI	ACNGT	6 cut(s)	2050, 3157, 3444, 3523, 3560, 3584
Bst6I	CTCTTC	5 cut(s)	1896, 2816, 3002, 3224, 3257
BstACI	GRCGYC	1 cut(s)	3270
BstAUI	TGTACA	1 cut(s)	3144
BstBAI	YACGTR	3 cut(s)	431, 805, 3150
BstC8I	GCNNGC	9 cut(s)	1875, 2157, 2237, 2439, 2615, 2667, 2850, 3005, 3090
BstDSI	CCRYGG	3 cut(s)	354, 1351, 1908
BstF5I	GGATG	8 cut(s)	324, 539, 1533, 2461, 2831, 2938, 3026, 3030
BstFNI	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
BstH2I	RGCGCY	3 cut(s)	312, 3080, 3273
BstHHI	GCGC	7 cut(s)	311, 320, 350, 2944, 3079, 3272, 3538
BstMCI	CGRYCG	1 cut(s)	3515
BstNI	CCWGG	3 cut(s)	105, 2930, 3323
BstNSI	RCATGY	4 cut(s)	1195, 2232, 2617, 3396
BstSCI	CCNGG	4 cut(s)	103, 1334, 2928, 3321
BstSLI	GKGCMC	3 cut(s)	2733, 3155, 3464
BstSNI	TACGTA	1 cut(s)	805
BstUI	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
BstV1I	GCAGC	6 cut(s)	15, 1082, 1649, 1664, 2498, 3557
BstV2I	GAAGAC	2 cut(s)	693, 1986
BstX2I	RGATCY	5 cut(s)	42, 1717, 2083, 2754, 3503
BstXI	CCANNNNNNTGG	1 cut(s)	2608
BstYI	RGATCY	5 cut(s)	42, 1717, 2083, 2754, 3503
BstZ17I	GTATAC	1 cut(s)	198
BstZI	CGGCCG	1 cut(s)	3512
Bsu15I	ATCGAT	1 cut(s)	2055
BsuI	GTATCC	1 cut(s)	3441
BsuTUI	ATCGAT	1 cut(s)	2055
BtgI	CCRYGG	3 cut(s)	354, 1351, 1908
BtgZI	GCGATG	2 cut(s)	2308, 2776
BtrI	CACGTC	1 cut(s)	2608
BtsCI	GGATG	8 cut(s)	324, 539, 1533, 2461, 2831, 2938, 3026, 3030
BtsI	GCAGTG	2 cut(s)	1703, 3294
BtsIMutI	CAGTG	8 cut(s)	270, 825, 1703, 1728, 2055, 2537, 3294, 3449
BveI	ACCTGC	1 cut(s)	1307
Cac8I	GCNNGC	9 cut(s)	1875, 2157, 2237, 2439, 2615, 2667, 2850, 3005, 3090
CaiI	CAGNNNCTG	1 cut(s)	1727
CciI	TCATGA	2 cut(s)	1002, 1494
CciNI	GCGGCCGC	1 cut(s)	3512
CfoI	GCGC	7 cut(s)	311, 320, 350, 2944, 3079, 3272, 3538
Cfr13I	GGNCC	6 cut(s)	101, 830, 954, 1947, 3325, 3552
Cfr42I	CCGCGG	1 cut(s)	1354
ClaI	ATCGAT	1 cut(s)	2055
CseI	GACGC	1 cut(s)	2705
DinI	GGCGCC	1 cut(s)	3271
DraI	TTTAAA	3 cut(s)	603, 1480, 2478
DrdI	GACNNNNNNGTC	1 cut(s)	1490
DseDI	GACNNNNNNGTC	1 cut(s)	1490
EaeI	YGGCCR	5 cut(s)	249, 1255, 1332, 1348, 3512
EagI	CGGCCG	1 cut(s)	3512
Eam1104I	CTCTTC	5 cut(s)	1896, 2816, 3002, 3224, 3257
EarI	CTCTTC	5 cut(s)	1896, 2816, 3002, 3224, 3257
Ecl136II	GAGCTC	1 cut(s)	1061
EclXI	CGGCCG	1 cut(s)	3512
Eco105I	TACGTA	1 cut(s)	805
Eco130I	CCWWGG	3 cut(s)	354, 1908, 2328
Eco147I	AGGCCT	1 cut(s)	3092
Eco24I	GRGCYC	1 cut(s)	1063
Eco31I	GGTCTC	2 cut(s)	566, 1898
Eco32I	GATATC	1 cut(s)	2632
Eco47I	GGWCC	4 cut(s)	101, 830, 954, 1947
Eco47III	AGCGCT	2 cut(s)	310, 3078
Eco52I	CGGCCG	1 cut(s)	3512
Eco53kI	GAGCTC	1 cut(s)	1061
Eco57I	CTGAAG	3 cut(s)	1145, 1383, 2983
Eco72I	CACGTG	1 cut(s)	3150
Eco88I	CYCGRG	1 cut(s)	3131
EcoICRI	GAGCTC	1 cut(s)	1061
EcoO109I	RGGNCCY	1 cut(s)	1947
EcoRI	GAATTC	3 cut(s)	509, 1673, 2023
EcoRII	CCWGG	3 cut(s)	103, 2928, 3321
EcoRV	GATATC	1 cut(s)	2632
EcoT14I	CCWWGG	3 cut(s)	354, 1908, 2328
EcoT38I	GRGCYC	1 cut(s)	1063
EgeI	GGCGCC	1 cut(s)	3271
EheI	GGCGCC	1 cut(s)	3271
ErhI	CCWWGG	3 cut(s)	354, 1908, 2328
Esp3I	CGTCTC	2 cut(s)	757, 2723
FaqI	GGGAC	3 cut(s)	114, 1351, 1439
FauI	CCCGC	3 cut(s)	633, 2387, 3337
FauNDI	CATATG	1 cut(s)	1612
FbaI	TGATCA	1 cut(s)	2418
FblI	GTMKAC	3 cut(s)	197, 1263, 1384
FokI	GGATG	8 cut(s)	311, 526, 1520, 2468, 2818, 2945, 3033, 3037
FriOI	GRGCYC	1 cut(s)	1063
FspBI	CTAG	3 cut(s)	1035, 2547, 2582
GlaI	GCGC	7 cut(s)	310, 319, 349, 2943, 3078, 3271, 3537
GsuI	CTGGAG	1 cut(s)	2211
HaeII	RGCGCY	3 cut(s)	312, 3080, 3273
HapII	CCGG	6 cut(s)	137, 1335, 1580, 2282, 2903, 3117
HgaI	GACGC	1 cut(s)	2705
HhaI	GCGC	7 cut(s)	311, 320, 350, 2944, 3079, 3272, 3538
Hin1I	GRCGYC	1 cut(s)	3270
Hin6I	GCGC	7 cut(s)	309, 318, 348, 2942, 3077, 3270, 3536
HinP1I	GCGC	7 cut(s)	309, 318, 348, 2942, 3077, 3270, 3536
HincII	GTYRAC	2 cut(s)	1264, 1385
HindII	GTYRAC	2 cut(s)	1264, 1385
HindIII	AAGCTT	1 cut(s)	2153
HinfI	GANTC	6 cut(s)	140, 647, 779, 1439, 1622, 2276
HpaII	CCGG	6 cut(s)	137, 1335, 1580, 2282, 2903, 3117
HphI	GGTGA	7 cut(s)	370, 418, 1389, 2514, 2603, 3193, 3270
Hpy99I	CGWCG	1 cut(s)	1389
HpyCH4III	ACNGT	6 cut(s)	2050, 3157, 3444, 3523, 3560, 3584
HpyCH4IV	ACGT	6 cut(s)	430, 804, 1748, 2607, 2883, 3149
HpySE526I	ACGT	6 cut(s)	430, 804, 1748, 2607, 2883, 3149
Hsp92I	GRCGYC	1 cut(s)	3270
HspAI	GCGC	7 cut(s)	309, 318, 348, 2942, 3077, 3270, 3536
KasI	GGCGCC	1 cut(s)	3269
Kpn2I	TCCGGA	2 cut(s)	1579, 2281
KpnI	GGTACC	1 cut(s)	3250
Ksp22I	TGATCA	1 cut(s)	2418
KspI	CCGCGG	1 cut(s)	1354
LmnI	GCTCC	7 cut(s)	304, 812, 1066, 2597, 2622, 2713, 3351
Lsp1109I	GCAGC	6 cut(s)	15, 1082, 1649, 1664, 2498, 3557
LweI	GCATC	9 cut(s)	147, 391, 544, 1039, 1176, 1924, 2342, 2396, 2507
MaeI	CTAG	3 cut(s)	1035, 2547, 2582
MaeII	ACGT	6 cut(s)	430, 804, 1748, 2607, 2883, 3149
MaeIII	GTNAC	9 cut(s)	268, 625, 2062, 2542, 2744, 3194, 3276, 3485, 3578
MbiI	CCGCTC	2 cut(s)	2394, 3374
MfeI	CAATTG	2 cut(s)	2487, 3234
MflI	RGATCY	5 cut(s)	42, 1717, 2083, 2754, 3503
MhlI	GDGCHC	4 cut(s)	1063, 2733, 3155, 3464
MlsI	TGGCCA	2 cut(s)	251, 1257
MluNI	TGGCCA	2 cut(s)	251, 1257
Mly113I	GGCGCC	1 cut(s)	3270
MlyI	GAGTC	3 cut(s)	773, 1433, 2285
MmeI	TCCRAC	6 cut(s)	542, 600, 697, 1695, 2277, 2620
Mox20I	TGGCCA	2 cut(s)	251, 1257
MroI	TCCGGA	2 cut(s)	1579, 2281
MroXI	GAANNNNTTC	4 cut(s)	513, 744, 1393, 1544
MscI	TGGCCA	2 cut(s)	251, 1257
MslI	CAYNNNNRTG	1 cut(s)	1920
Msp20I	TGGCCA	2 cut(s)	251, 1257
MspA1I	CMGCKG	2 cut(s)	1353, 3003
MspI	CCGG	6 cut(s)	137, 1335, 1580, 2282, 2903, 3117
MspR9I	CCNGG	4 cut(s)	105, 1336, 2930, 3323
MunI	CAATTG	2 cut(s)	2487, 3234
Mva1269I	GAATGC	2 cut(s)	700, 2667
MvaI	CCWGG	3 cut(s)	105, 2930, 3323
MvnI	CGCG	5 cut(s)	320, 350, 1353, 2944, 3538
NarI	GGCGCC	1 cut(s)	3270
NciI	CCSGG	1 cut(s)	1336
NcoI	CCATGG	2 cut(s)	354, 1908
NdeI	CATATG	1 cut(s)	1612
NmuCI	GTSAC	4 cut(s)	268, 2542, 3276, 3485
NotI	GCGGCCGC	1 cut(s)	3512
NspI	RCATGY	4 cut(s)	1195, 2232, 2617, 3396
PaeI	GCATGC	1 cut(s)	2617
PagI	TCATGA	2 cut(s)	1002, 1494
PceI	AGGCCT	1 cut(s)	3092
PciI	ACATGT	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	1 cut(s)	3146
PctI	GAATGC	2 cut(s)	700, 2667
PdmI	GAANNNNTTC	4 cut(s)	513, 744, 1393, 1544
PfeI	GAWTC	3 cut(s)	140, 647, 1622
Pfl23II	CGTACG	1 cut(s)	3264
PflMI	CCANNNNNTGG	1 cut(s)	1727
PleI	GAGTC	3 cut(s)	773, 1433, 2284
PluTI	GGCGCC	1 cut(s)	3273
PmaCI	CACGTG	1 cut(s)	3150
PmlI	CACGTG	1 cut(s)	3150
PpsI	GAGTC	3 cut(s)	773, 1433, 2284
Ppu21I	YACGTR	3 cut(s)	431, 805, 3150
PpuMI	RGGWCCY	1 cut(s)	1947
PscI	ACATGT	1 cut(s)	1191
PsiI	TTATAA	1 cut(s)	2880
Psp124BI	GAGCTC	1 cut(s)	1063
Psp5II	RGGWCCY	1 cut(s)	1947
Psp6I	CCWGG	3 cut(s)	103, 2928, 3321
PspCI	CACGTG	1 cut(s)	3150
PspGI	CCWGG	3 cut(s)	103, 2928, 3321
PspLI	CGTACG	1 cut(s)	3264
PspPI	GGNCC	6 cut(s)	101, 830, 954, 1947, 3325, 3552
PspPPI	RGGWCCY	1 cut(s)	1947
PstNI	CAGNNNCTG	1 cut(s)	1727
PsuI	RGATCY	5 cut(s)	42, 1717, 2083, 2754, 3503
PvuII	CAGCTG	1 cut(s)	3003
RseI	CAYNNNNRTG	1 cut(s)	1920
SacI	GAGCTC	1 cut(s)	1063
SacII	CCGCGG	1 cut(s)	1354
SalI	GTCGAC	2 cut(s)	1262, 1383
Sau96I	GGNCC	6 cut(s)	101, 830, 954, 1947, 3325, 3552
SchI	GAGTC	3 cut(s)	773, 1433, 2285
ScrFI	CCNGG	4 cut(s)	105, 1336, 2930, 3323
SduI	GDGCHC	4 cut(s)	1063, 2733, 3155, 3464
SfaNI	GCATC	9 cut(s)	147, 391, 544, 1039, 1176, 1924, 2342, 2396, 2507
SfoI	GGCGCC	1 cut(s)	3271
Sfr303I	CCGCGG	1 cut(s)	1354
SgrBI	CCGCGG	1 cut(s)	1354
SinI	GGWCC	4 cut(s)	101, 830, 954, 1947
SmiMI	CAYNNNNRTG	1 cut(s)	1920
SmlI	CTYRAG	3 cut(s)	1433, 1973, 2954
SmoI	CTYRAG	3 cut(s)	1433, 1973, 2954
SnaBI	TACGTA	1 cut(s)	805
SphI	GCATGC	1 cut(s)	2617
SseBI	AGGCCT	1 cut(s)	3092
SspDI	GGCGCC	1 cut(s)	3269
SspI	AATATT	3 cut(s)	1830, 2218, 3015
SspMI	CTAG	3 cut(s)	1035, 2547, 2582
SstI	GAGCTC	1 cut(s)	1063
StuI	AGGCCT	1 cut(s)	3092
StyD4I	CCNGG	4 cut(s)	103, 1334, 2928, 3321
StyI	CCWWGG	3 cut(s)	354, 1908, 2328
TaaI	ACNGT	6 cut(s)	2050, 3157, 3444, 3523, 3560, 3584
TaiI	ACGT	6 cut(s)	433, 807, 1751, 2610, 2886, 3152
TatI	WGTACW	5 cut(s)	188, 705, 1194, 1969, 3144
TauI	GCSGC	6 cut(s)	1353, 1356, 3374, 3499, 3514, 3517
TfiI	GAWTC	3 cut(s)	140, 647, 1622
TscAI	CASTG	8 cut(s)	277, 832, 1710, 1728, 2055, 2544, 3301, 3449
TseFI	GTSAC	4 cut(s)	268, 2542, 3276, 3485
TseI	GCWGC	6 cut(s)	28, 1070, 1637, 1652, 2511, 3545
Tsp45I	GTSAC	4 cut(s)	268, 2542, 3276, 3485
TspGWI	ACGGA	3 cut(s)	183, 1053, 3086
TspRI	CASTG	8 cut(s)	277, 832, 1710, 1728, 2055, 2544, 3301, 3449
Van91I	CCANNNNNTGG	1 cut(s)	1727
VneI	GTGCAC	2 cut(s)	2729, 3151
VpaK11BI	GGWCC	4 cut(s)	101, 830, 954, 1947
XbaI	TCTAGA	1 cut(s)	1034
XceI	RCATGY	4 cut(s)	1195, 2232, 2617, 3396
XmiI	GTMKAC	3 cut(s)	197, 1263, 1384
XmnI	GAANNNNTTC	4 cut(s)	513, 744, 1393, 1544
XspI	CTAG	3 cut(s)	1035, 2547, 2582

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.