Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

57147917 .. 57153332

Gene Structure 5416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G030420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3483 bp

ATGAGGAGCGGTGGGAGAAAGAGAAGGCTGCATTTCAGCAAGATCTATTCTTTCACTTGCGGTAGAGCTTCTTTGAAGGAGGAGCATTCACAGATTGGAGGGCCAGGATTTTCCAGAGTGGTTTTCTGCAACGAACCGGATTCGTTTGAGGCCGGGATTCGAAACTATGCAGATAATTATGTCAGCACTACCAAGTACACACTTGCTACATTCTTGCCCAAGTCTTTGTTTGAGCAGTTCAGGAGGGTAGCTAACTTCTTCTTCTTGGTCACTGGCGTTTTGGCCTTCACTCCTCTGGCTCCGTATTCAGCTGTCAGTGCCATAATTCCTCTGATCATCATCATTAGCGCCACGATGGTGAAGGAGGGGATCGAAGACTGGCGTCGAAAACAGCAGGATATTGAAGTGAACAATAGAAAGGTTAAAGTGCATAAGGGTAGTGGCAATTTTGATTATACTGAATGGAAGAATCTGAGAGTGGGAGATATAGTGAGAGTGGAAAAGAATGAATTCTTTCCAACAGATCTCCTGTTACTTTCGTCCAGTTACGAAGATGCAATCTGCTATGTTGAGACAATGAACCTTGATGGGGAGACAAACTTAAAGTTGAAACAAGCATTGGAGGTAACTTCACCGTTACAGGAGGACACCAGCATCAGCAATTTTCATGCCATGGTGAAATGTGAGGACCCAAATGCAAATTTGTACTCATTTGTCGGAACTATGGAGTTTGAAAAGCAGCAGTATCCCCTCTCTCCTCAACAGCTTCTTCTCAGAGACTCTAAACTCCGAAATACAGACTACATATACGGAGTGGTTATCTTCACCGGTGTTGATACAAAGGTTATTCAAAACTCTACTCCCCCTCCTTCAAAAAGAAGCAGAGTTGAGAAGAAGATGGATCAAATTATCTACGTCTTGTTTGGTGTTTTATTTACATTGGCATCAGTTGGATCCATTTTCTTTGGCATTACAACCAAAGATGACATAAACAATGGGATCATGAAAAGGTGGTATCTGAAGCCAGATAGTTCTGAAATTTTCTATGATCCCAAAAGGGCTCCTGTTGCAGCAATTTATCACTTCTTGACTGCTCTGATGTTATATAACTACTTGATCCCTATCTCCTTGTATGTGTCAATCGAAATTGTTAAAGTTCTTCAGAGCGTCTTCATCAATCAAGATATTCAAATGTACTATGAAGAAGCTGATAAACCAGCACATGCCCGTACCTCAAACTTGAATGAAGAACTTGGCCAAGTTGACACAATTCTTTCAGACAAGACTGGAACTTTGACATGCAATTCAATGGAGTTCATCAAATGTTCTGTGGCTGGAACAGCTTACGGCCGTGGTTTTACTGAGGTTGAGAGGTCTATGGGCAGGCGAAATGGTTCACCAATTCATGAAGCTGTAAGTGGAGGGAATGATGACACGGCACCCGATCATGAATGGAATTGGGTTGATGAGCCTCATGGAGATCTCATCCAGAAATTTTTCCGCTTATTAGCAGTCTGCCATACAGCCATTCCAGAAGTTGATGAAGTAACTGGGAAGGTTATGTATGAGGCTGAGTCTCCTGATGAAGCAGCATTTGTGATTGCAGCAAGAGAACTTGGTTTTGAATTCTATAAAAGGACACAAACAAGCATATCGGTGAGAGAATTGGACAGAGCCTCTGGAAAGAAAGTTGATAGGTTGTACACTCTCCTGAATGTGTTAGAATTCAACAGCTCAAGGAAACGGATGTCTGTGATTGTAAGAAATGAGGAAGGAAAAGTACTATTGCTCTGTAAAGGTGCCGACAATGTCATGTTTGAAAGACTTGCCAAAAATGGTAGGGAGTTTGAAGAAGAGACCAAGGAGCATTTAAATGGGTATGCTGATGCAGGGCTAAGGACTTTGATACTTGCCTATCGTGAACTTCAGGAGGATGAATACAGAGAGTTTAATGCAAAATTTATCAAGGCCAAGAACTCAATTAGTGCAGACCACGAAACATTGATTGATGAAGTAACAGATAATGTTGAAAAAGATCTAATTCTTCTTGGTGCGACAGCTGTTGAGGACAAACTTCAAAATGGGATTTGGGTTTTGACTGGAGACAAGATGGAAACTGCCATCAACATTGGGTTTGCCTGTAGCTTACTTAGACAAGGAATGAAACAGGTTATGATAAATTTGGAGTCTCCAGAAATTAAGGTGCTGGAAAAGGAAGGAGATAAGGATGCCATTACCAAGGCATCAAGAGCACGTGTCCTCCATCAGATAACCAAGGGAAAGGCCCAGCTTACAGCATCAAGTGGGGGATCCGAGGCATATGCATTGATCATTGATGGAAAATCTCTTGCTTATGCTCTTGAGGATGATATAAAGTCATTGTTTCTAGAGCTTGCAATGGGCTGTGCATCTGTTATTTGTTGCCGTTCGTCCCCAAAACAGAAAGCACTGGTCACTAGACTGGTCAAATCTGGAACTGGGAAAACAACTCTTGCGATTGGTGATGGCGCCAATGATGTGGGAATGCTCCAAGAAGCAGATATTGGGATTGGAATTAGTGGTGTTGAAGGAATGCAGGCGGTCATGTCAAGTGATATTGCGATTGCACAATTTCAATATTTAGAGCGTCTGCTGCTTGTGCATGGACATTGGTGTTACAGAAGGATCTCATCAATGATTTGCTACTTCTTCTACAAGAACATCACATTTGGTTTAACTATCTTCTTCTATGAGGCATCCGCATCCTTCTCTGGACAACCTGCATACAATGACTGGTTTTTATCACTGTATAATGTATTCTTCTCATCGCTTCCAGTGGTTGCCATGGGAGTTTTCGACCAGGATGTTTCTGCACGATTTTGTCTCAAGTTTCCTCTAATATACCAAGAAGGGGTGCAGAATGTTCTCTTCAGCTGGCGTAGAATAATTGGTTGGATGTTGAATGGATTAACAAGCGCAGTGATTATTTTCTTTTTCTGCATGAAAGCGCTACAACCATATGCCTTCAATCAGGATGGGAAAACTGCTGGGAAGGATATTCTAGGAGCAACAATGTACACATGCATCGTTTGGGTTGTGAACTTGCAGATGGCGCTTGCCATAAGTTACTTCACCTTGATACAACACCTTTTCATCTGGGGTTCCATTGCTTTCTGGTATCTTTTTATGTTAGCATATGGAGCCATGTCACCCACGTTCTCTACCAATGCATACAAAGTCTTCGTCGAAACCCTTGCCCCCGCCCCTTCTTTCTGGTTTATTACATTGCTTGTGCCAATCTCAGCTCTACTCCCATATTTCACATACTCATCACTTTGGATGCGGTTCTTTCCTTTGTATCATGAAATGATACAATGGATTAGGTACGAGGGGCGGTCAAATGATCCAGAGTTTTGTGATATGGTGCGGCAGAGATCTTTGCGGCCAACAACTGTGGGTTTCACAGCACGGTTAGCTGCTAGGACCAACATTATAAGAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1160

Amino Acids

131.19

Weight (kDa)

6.27

Isoelectric Point (pI)

38.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 108	2.9e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	497 - 693	4e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	861 - 1111	2e-85	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3473
Acc36I	ACCTGC	1 cut(s)	2767
AccB1I	GGYRCC	3 cut(s)	1438, 1799, 2507
AccBSI	CCGCTC	1 cut(s)	9
AcoI	YGGCCR	3 cut(s)	1255, 1348, 3422
AcsI	RAATTY	8 cut(s)	509, 700, 1038, 1493, 1625, 1724, 1958, 2179
AcuI	CTGAAG	4 cut(s)	1040, 1145, 1910, 2893
AcvI	CACGTG	1 cut(s)	2255
AcyI	GRCGYC	2 cut(s)	382, 2508
AfaI	GTAC	8 cut(s)	197, 707, 1196, 1231, 1703, 1782, 3056, 3365
AfeI	AGCGCT	1 cut(s)	2988
AfiI	CCNNNNNNNGG	2 cut(s)	589, 2890
AflIII	ACRYGT	1 cut(s)	2254
AgeI	ACCGGT	1 cut(s)	827
AjnI	CCWGG	2 cut(s)	103, 2838
AjuI	GAANNNNNNNTTGG	6 cut(s)	602, 634, 2526, 2558, 2914, 2946
AleI	CACNNNNGTG	1 cut(s)	356
Alw21I	GWGCWC	1 cut(s)	2254
Alw26I	GTCTC	8 cut(s)	566, 587, 771, 1581, 1850, 2097, 2193, 2867
Aor51HI	AGCGCT	1 cut(s)	2988
AoxI	GGCC	8 cut(s)	101, 150, 282, 1255, 1348, 1968, 2283, 3422
ApeKI	GCWGC	7 cut(s)	28, 739, 1070, 1589, 1604, 2632, 3455
ApoI	RAATTY	8 cut(s)	509, 700, 1038, 1493, 1625, 1724, 1958, 2179
ArsI	GACNNNNNNTTYG	4 cut(s)	1276, 1308, 3207, 3239
AsiGI	ACCGGT	1 cut(s)	827
Asp700I	GAANNNNTTC	4 cut(s)	509, 513, 1393, 1496
AspLEI	GCGC	5 cut(s)	350, 2510, 2957, 2989, 3094
AspS9I	GGNCC	4 cut(s)	101, 688, 2284, 3462
AsuC2I	CCSGG	1 cut(s)	154
AsuHPI	GGTGA	9 cut(s)	370, 624, 688, 817, 1389, 1669, 2513, 3103, 3180
AsuII	TTCGAA	1 cut(s)	160
AvaII	GGWCC	2 cut(s)	688, 3462
BaeI	ACNNNNGTAYC	2 cut(s)	828, 861
BalI	TGGCCA	1 cut(s)	1257
BamHI	GGATCC	2 cut(s)	953, 2309
BanI	GGYRCC	3 cut(s)	1438, 1799, 2507
BanII	GRGCYC	1 cut(s)	1063
BarI	GAAGNNNNNNTAC	2 cut(s)	1764, 1796
BbrPI	CACGTG	1 cut(s)	2255
BbsI	GAAGAC	3 cut(s)	381, 1162, 3211
Bbv12I	GWGCWC	1 cut(s)	2254
BbvI	GCAGC	7 cut(s)	15, 751, 1082, 1601, 1616, 2619, 3442
BceAI	ACGGC	4 cut(s)	1335, 1363, 1452, 2409
BcgI	CGANNNNNNTGC	4 cut(s)	2303, 2337, 3215, 3249
BciT130I	CCWGG	2 cut(s)	105, 2840
BciVI	GTATCC	1 cut(s)	756
BclI	TGATCA	2 cut(s)	333, 2328
BcnI	CCSGG	1 cut(s)	154
BcoDI	GTCTC	8 cut(s)	566, 587, 771, 1581, 1850, 2097, 2193, 2867
BfaI	CTAG	4 cut(s)	2387, 2457, 3041, 3459
BfmI	CTRYAG	1 cut(s)	2140
BfoI	RGCGCY	4 cut(s)	351, 2511, 2990, 3095
BfuAI	ACCTGC	1 cut(s)	2767
BfuI	GTATCC	1 cut(s)	756
BglII	AGATCT	5 cut(s)	42, 523, 1480, 2035, 3413
BisI	GCNGC	9 cut(s)	29, 740, 1071, 1590, 1605, 2633, 3407, 3422, 3456
BlsI	GCNGC	9 cut(s)	30, 741, 1072, 1591, 1606, 2634, 3408, 3423, 3457
BmcAI	AGTACT	1 cut(s)	1782
Bme1390I	CCNGG	3 cut(s)	105, 154, 2840
Bme18I	GGWCC	2 cut(s)	688, 3462
BmgT120I	GGNCC	4 cut(s)	101, 688, 2284, 3462
BmrFI	CCNGG	3 cut(s)	105, 154, 2840
BmrI	ACTGGG	2 cut(s)	1560, 2487
BmuI	ACTGGG	2 cut(s)	1560, 2487
BoxI	GACNNNNGTC	1 cut(s)	381
BpiI	GAAGAC	3 cut(s)	381, 1162, 3211
BpmI	CTGGAG	2 cut(s)	2121, 2175
Bpu10I	CCTNAGC	1 cut(s)	1895
Bpu14I	TTCGAA	1 cut(s)	160
BpuEI	CTTGAG	3 cut(s)	1720, 2381, 2849
BpuMI	CCSGG	1 cut(s)	154
BsaAI	YACGTR	1 cut(s)	2255
BsaBI	GATNNNNATC	2 cut(s)	338, 1121
BsaHI	GRCGYC	2 cut(s)	382, 2508
BsaI	GGTCTC	1 cut(s)	1850
BsaJI	CCNNGG	7 cut(s)	672, 1351, 1860, 2238, 2274, 2313, 2823
BsaWI	WCCGGW	2 cut(s)	136, 827
BsaXI	ACNNNNNCTCC	6 cut(s)	474, 504, 850, 880, 2244, 2274
Bsc4I	CCNNNNNNNGG	2 cut(s)	589, 2890
Bse118I	RCCGGY	1 cut(s)	827
Bse3DI	GCAATG	3 cut(s)	2403, 3144, 3263
Bse8I	GATNNNNATC	2 cut(s)	338, 1121
BseBI	CCWGG	2 cut(s)	105, 2840
BseDI	CCNNGG	7 cut(s)	672, 1351, 1860, 2238, 2274, 2313, 2823
BseJI	GATNNNNATC	2 cut(s)	338, 1121
BseLI	CCNNNNNNNGG	2 cut(s)	589, 2890
BseMI	GCAATG	3 cut(s)	2403, 3144, 3263
BseMII	CTCAG	5 cut(s)	464, 787, 1353, 1563, 3294
BseRI	GAGGAG	4 cut(s)	19, 95, 282, 747
BseX3I	CGGCCG	1 cut(s)	1348
BseXI	GCAGC	7 cut(s)	15, 751, 1082, 1601, 1616, 2619, 3442
BseYI	CCCAGC	2 cut(s)	2286, 3026
BsgI	GTGCAG	3 cut(s)	2007, 2835, 2915
Bsh1285I	CGRYCG	1 cut(s)	1351
BshFI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 1970, 2285, 3424
BshNI	GGYRCC	3 cut(s)	1438, 1799, 2507
BshTI	ACCGGT	1 cut(s)	827
BsiEI	CGRYCG	1 cut(s)	1351
BsiHKAI	GWGCWC	1 cut(s)	2254
BsiSI	CCGG	3 cut(s)	137, 153, 828
BslFI	GGGAC	1 cut(s)	2416
BslI	CCNNNNNNNGG	2 cut(s)	589, 2890
BsmAI	GTCTC	8 cut(s)	566, 587, 771, 1581, 1850, 2097, 2193, 2867
BsmFI	GGGAC	1 cut(s)	2416
BsmI	GAATGC	3 cut(s)	85, 2529, 2577
BsnI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 1970, 2285, 3424
Bso31I	GGTCTC	1 cut(s)	1850
Bsp119I	TTCGAA	1 cut(s)	160
Bsp1286I	GDGCHC	2 cut(s)	1063, 2254
Bsp1407I	TGTACA	2 cut(s)	1701, 3054
Bsp19I	CCATGG	2 cut(s)	672, 2823
BspANI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 1970, 2285, 3424
BspCNI	CTCAG	5 cut(s)	465, 786, 1354, 1564, 3293
BspHI	TCATGA	4 cut(s)	1002, 1405, 1447, 3340
BspMI	ACCTGC	1 cut(s)	2767
BspT104I	TTCGAA	1 cut(s)	160
BspT107I	GGYRCC	3 cut(s)	1438, 1799, 2507
BspTNI	GGTCTC	1 cut(s)	1850
BsrBI	CCGCTC	1 cut(s)	9
BsrDI	GCAATG	3 cut(s)	2403, 3144, 3263
BsrFI	RCCGGY	1 cut(s)	827
BsrGI	TGTACA	2 cut(s)	1701, 3054
BssAI	RCCGGY	1 cut(s)	827
BssECI	CCNNGG	7 cut(s)	672, 1351, 1860, 2238, 2274, 2313, 2823
BssNI	GRCGYC	2 cut(s)	382, 2508
BssT1I	CCWWGG	5 cut(s)	672, 1860, 2238, 2274, 2823
Bst2UI	CCWGG	2 cut(s)	105, 2840
Bst4CI	ACNGT	4 cut(s)	636, 2787, 3433, 3450
Bst6I	CTCTTC	2 cut(s)	1848, 2912
BstACI	GRCGYC	2 cut(s)	382, 2508
BstAUI	TGTACA	2 cut(s)	1701, 3054
BstBAI	YACGTR	1 cut(s)	2255
BstBI	TTCGAA	1 cut(s)	160
BstC8I	GCNNGC	5 cut(s)	1385, 2394, 2577, 2915, 3096
BstDEI	CTNAG	7 cut(s)	473, 773, 1362, 1572, 1895, 2150, 3280
BstDSI	CCRYGG	3 cut(s)	672, 1351, 2823
BstH2I	RGCGCY	4 cut(s)	351, 2511, 2990, 3095
BstHHI	GCGC	5 cut(s)	350, 2510, 2957, 2989, 3094
BstMAI	GTCTC	8 cut(s)	566, 587, 771, 1581, 1850, 2097, 2193, 2867
BstMCI	CGRYCG	1 cut(s)	1351
BstMWI	GCNNNNNNNGC	9 cut(s)	317, 1067, 1340, 2249, 2632, 2638, 3091, 3179, 3452
BstNI	CCWGG	2 cut(s)	105, 2840
BstNSI	RCATGY	3 cut(s)	1226, 1302, 3063
BstPAI	GACNNNNGTC	1 cut(s)	381
BstSCI	CCNGG	3 cut(s)	103, 152, 2838
BstSFI	CTRYAG	1 cut(s)	2140
BstV1I	GCAGC	7 cut(s)	15, 751, 1082, 1601, 1616, 2619, 3442
BstV2I	GAAGAC	3 cut(s)	381, 1162, 3211
BstX2I	RGATCY	8 cut(s)	42, 523, 953, 1480, 2035, 2309, 2664, 3413
BstXI	CCANNNNNNTGG	1 cut(s)	2518
BstYI	RGATCY	8 cut(s)	42, 523, 953, 1480, 2035, 2309, 2664, 3413
BstZI	CGGCCG	1 cut(s)	1348
BsuI	GTATCC	1 cut(s)	756
BsuRI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 1970, 2285, 3424
BtgI	CCRYGG	3 cut(s)	672, 1351, 2823
BtgZI	GCGATG	1 cut(s)	2790
BtsI	GCAGTG	1 cut(s)	2964
BtsIMutI	CAGTG	6 cut(s)	270, 322, 2447, 2783, 2820, 2964
BveI	ACCTGC	1 cut(s)	2767
Cac8I	GCNNGC	5 cut(s)	1385, 2394, 2577, 2915, 3096
CciI	TCATGA	4 cut(s)	1002, 1405, 1447, 3340
CfoI	GCGC	5 cut(s)	350, 2510, 2957, 2989, 3094
Cfr10I	RCCGGY	1 cut(s)	827
Cfr13I	GGNCC	4 cut(s)	101, 688, 2284, 3462
CseI	GACGC	3 cut(s)	371, 1156, 2615
Csp6I	GTAC	8 cut(s)	196, 706, 1195, 1230, 1702, 1781, 3055, 3364
CspAI	ACCGGT	1 cut(s)	827
CspCI	CAANNNNNGTGG	2 cut(s)	3414, 3449
CviQI	GTAC	8 cut(s)	196, 706, 1195, 1230, 1702, 1781, 3055, 3364
DdeI	CTNAG	7 cut(s)	473, 773, 1362, 1572, 1895, 2150, 3280
DinI	GGCGCC	1 cut(s)	2509
DraI	TTTAAA	1 cut(s)	1872
EaeI	YGGCCR	3 cut(s)	1255, 1348, 3422
EagI	CGGCCG	1 cut(s)	1348
Eam1104I	CTCTTC	2 cut(s)	1848, 2912
EarI	CTCTTC	2 cut(s)	1848, 2912
EclXI	CGGCCG	1 cut(s)	1348
Eco130I	CCWWGG	5 cut(s)	672, 1860, 2238, 2274, 2823
Eco24I	GRGCYC	1 cut(s)	1063
Eco31I	GGTCTC	1 cut(s)	1850
Eco47I	GGWCC	2 cut(s)	688, 3462
Eco47III	AGCGCT	1 cut(s)	2988
Eco52I	CGGCCG	1 cut(s)	1348
Eco57I	CTGAAG	4 cut(s)	1040, 1145, 1910, 2893
Eco72I	CACGTG	1 cut(s)	2255
EcoO109I	RGGNCCY	1 cut(s)	688
EcoRI	GAATTC	3 cut(s)	509, 1625, 1724
EcoRII	CCWGG	2 cut(s)	103, 2838
EcoT14I	CCWWGG	5 cut(s)	672, 1860, 2238, 2274, 2823
EcoT22I	ATGCAT	3 cut(s)	2326, 3065, 3211
EcoT38I	GRGCYC	1 cut(s)	1063
EgeI	GGCGCC	1 cut(s)	2509
EheI	GGCGCC	1 cut(s)	2509
ErhI	CCWWGG	5 cut(s)	672, 1860, 2238, 2274, 2823
FaqI	GGGAC	1 cut(s)	2416
FauI	CCCGC	1 cut(s)	3247
FauNDI	CATATG	3 cut(s)	2320, 2998, 3175
FbaI	TGATCA	2 cut(s)	333, 2328
Fnu4HI	GCNGC	9 cut(s)	29, 740, 1071, 1590, 1605, 2633, 3407, 3422, 3456
FriOI	GRGCYC	1 cut(s)	1063
Fsp4HI	GCNGC	9 cut(s)	29, 740, 1071, 1590, 1605, 2633, 3407, 3422, 3456
FspBI	CTAG	4 cut(s)	2387, 2457, 3041, 3459
GlaI	GCGC	5 cut(s)	349, 2509, 2956, 2988, 3093
GluI	GCNGC	9 cut(s)	29, 740, 1071, 1590, 1605, 2633, 3407, 3422, 3456
GsaI	CCCAGC	2 cut(s)	2290, 3030
GsuI	CTGGAG	2 cut(s)	2121, 2175
HaeII	RGCGCY	4 cut(s)	351, 2511, 2990, 3095
HaeIII	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 1970, 2285, 3424
HapII	CCGG	3 cut(s)	137, 153, 828
HgaI	GACGC	3 cut(s)	371, 1156, 2615
HhaI	GCGC	5 cut(s)	350, 2510, 2957, 2989, 3094
Hin1I	GRCGYC	2 cut(s)	382, 2508
Hin6I	GCGC	5 cut(s)	348, 2508, 2955, 2987, 3092
HinP1I	GCGC	5 cut(s)	348, 2508, 2955, 2987, 3092
HincII	GTYRAC	1 cut(s)	1264
HindII	GTYRAC	1 cut(s)	1264
HinfI	GANTC	6 cut(s)	140, 157, 469, 779, 1574, 2186
HpaII	CCGG	3 cut(s)	137, 153, 828
HphI	GGTGA	9 cut(s)	370, 624, 688, 817, 1389, 1669, 2513, 3103, 3180
Hpy166II	GTNNAC	8 cut(s)	198, 409, 1264, 1397, 1704, 1922, 3057, 3079
Hpy8I	GTNNAC	8 cut(s)	198, 409, 1264, 1397, 1704, 1922, 3057, 3079
Hpy99I	CGWCG	2 cut(s)	387, 3227
HpyCH4III	ACNGT	4 cut(s)	636, 2787, 3433, 3450
HpyCH4IV	ACGT	3 cut(s)	915, 2254, 3194
HpyF10VI	GCNNNNNNNGC	9 cut(s)	317, 1067, 1340, 2249, 2632, 2638, 3091, 3179, 3452
HpyF3I	CTNAG	7 cut(s)	473, 773, 1362, 1572, 1895, 2150, 3280
HpySE526I	ACGT	3 cut(s)	915, 2254, 3194
Hsp92I	GRCGYC	2 cut(s)	382, 2508
HspAI	GCGC	5 cut(s)	348, 2508, 2955, 2987, 3092
KasI	GGCGCC	1 cut(s)	2507
Ksp22I	TGATCA	2 cut(s)	333, 2328
LmnI	GCTCC	8 cut(s)	6, 82, 304, 1066, 1864, 2532, 3044, 3179
Lsp1109I	GCAGC	7 cut(s)	15, 751, 1082, 1601, 1616, 2619, 3442
MaeI	CTAG	4 cut(s)	2387, 2457, 3041, 3459
MaeII	ACGT	3 cut(s)	915, 2254, 3194
MbiI	CCGCTC	1 cut(s)	9
MflI	RGATCY	8 cut(s)	42, 523, 953, 1480, 2035, 2309, 2664, 3413
MhlI	GDGCHC	2 cut(s)	1063, 2254
MlsI	TGGCCA	1 cut(s)	1257
MluNI	TGGCCA	1 cut(s)	1257
Mly113I	GGCGCC	1 cut(s)	2508
MlyI	GAGTC	3 cut(s)	773, 1583, 2195
MmeI	TCCRAC	4 cut(s)	542, 697, 931, 2912
Mox20I	TGGCCA	1 cut(s)	1257
Mph1103I	ATGCAT	3 cut(s)	2326, 3065, 3211
MroXI	GAANNNNTTC	4 cut(s)	509, 513, 1393, 1496
MscI	TGGCCA	1 cut(s)	1257
MseI	TTAA	8 cut(s)	423, 602, 1152, 1871, 1950, 2199, 2714, 2948
MslI	CAYNNNNRTG	3 cut(s)	356, 1655, 1872
Msp20I	TGGCCA	1 cut(s)	1257
MspA1I	CMGCKG	3 cut(s)	311, 2060, 2913
MspI	CCGG	3 cut(s)	137, 153, 828
MspR9I	CCNGG	3 cut(s)	105, 154, 2840
Mva1269I	GAATGC	3 cut(s)	85, 2529, 2577
MvaI	CCWGG	2 cut(s)	105, 2840
MwoI	GCNNNNNNNGC	9 cut(s)	317, 1067, 1340, 2249, 2632, 2638, 3091, 3179, 3452
NarI	GGCGCC	1 cut(s)	2508
NciI	CCSGG	1 cut(s)	154
NcoI	CCATGG	2 cut(s)	672, 2823
NdeI	CATATG	3 cut(s)	2320, 2998, 3175
NmuCI	GTSAC	3 cut(s)	268, 2452, 3186
NsiI	ATGCAT	3 cut(s)	2326, 3065, 3211
NspI	RCATGY	3 cut(s)	1226, 1302, 3063
NspV	TTCGAA	1 cut(s)	160
OliI	CACNNNNGTG	1 cut(s)	356
PagI	TCATGA	4 cut(s)	1002, 1405, 1447, 3340
PctI	GAATGC	3 cut(s)	85, 2529, 2577
PdmI	GAANNNNTTC	4 cut(s)	509, 513, 1393, 1496
PfeI	GAWTC	3 cut(s)	140, 157, 469
PflFI	GACNNNGTC	1 cut(s)	1808
PinAI	ACCGGT	1 cut(s)	827
PkrI	GCNGC	9 cut(s)	30, 741, 1072, 1591, 1606, 2634, 3408, 3423, 3457
PleI	GAGTC	3 cut(s)	773, 1582, 2194
PluTI	GGCGCC	1 cut(s)	2511
PmaCI	CACGTG	1 cut(s)	2255
PmlI	CACGTG	1 cut(s)	2255
PpsI	GAGTC	3 cut(s)	773, 1582, 2194
Ppu21I	YACGTR	1 cut(s)	2255
PpuMI	RGGWCCY	1 cut(s)	688
PshAI	GACNNNNGTC	1 cut(s)	381
PsiI	TTATAA	1 cut(s)	3473
Psp5II	RGGWCCY	1 cut(s)	688
Psp6I	CCWGG	2 cut(s)	103, 2838
PspCI	CACGTG	1 cut(s)	2255
PspFI	CCCAGC	2 cut(s)	2286, 3026
PspGI	CCWGG	2 cut(s)	103, 2838
PspPI	GGNCC	4 cut(s)	101, 688, 2284, 3462
PspPPI	RGGWCCY	1 cut(s)	688
PsuI	RGATCY	8 cut(s)	42, 523, 953, 1480, 2035, 2309, 2664, 3413
PsyI	GACNNNGTC	1 cut(s)	1808
PvuII	CAGCTG	3 cut(s)	311, 2060, 2913
RsaI	GTAC	8 cut(s)	197, 707, 1196, 1231, 1703, 1782, 3056, 3365
RsaNI	GTAC	8 cut(s)	196, 706, 1195, 1230, 1702, 1781, 3055, 3364
RseI	CAYNNNNRTG	3 cut(s)	356, 1655, 1872
SaqAI	TTAA	8 cut(s)	423, 602, 1152, 1871, 1950, 2199, 2714, 2948
SatI	GCNGC	9 cut(s)	29, 740, 1071, 1590, 1605, 2633, 3407, 3422, 3456
Sau96I	GGNCC	4 cut(s)	101, 688, 2284, 3462
ScaI	AGTACT	1 cut(s)	1782
SchI	GAGTC	3 cut(s)	773, 1583, 2195
ScrFI	CCNGG	3 cut(s)	105, 154, 2840
SduI	GDGCHC	2 cut(s)	1063, 2254
SfcI	CTRYAG	1 cut(s)	2140
SfoI	GGCGCC	1 cut(s)	2509
SfuI	TTCGAA	1 cut(s)	160
SgrAI	CRCCGGYG	1 cut(s)	827
SinI	GGWCC	2 cut(s)	688, 3462
SmiI	ATTTAAAT	1 cut(s)	1872
SmiMI	CAYNNNNRTG	3 cut(s)	356, 1655, 1872
SmlI	CTYRAG	3 cut(s)	1735, 2360, 2864
SmoI	CTYRAG	3 cut(s)	1735, 2360, 2864
SspDI	GGCGCC	1 cut(s)	2507
SspI	AATATT	1 cut(s)	2618
SspMI	CTAG	4 cut(s)	2387, 2457, 3041, 3459
StyD4I	CCNGG	3 cut(s)	103, 152, 2838
StyI	CCWWGG	5 cut(s)	672, 1860, 2238, 2274, 2823
SwaI	ATTTAAAT	1 cut(s)	1872
TaaI	ACNGT	4 cut(s)	636, 2787, 3433, 3450
TaiI	ACGT	3 cut(s)	918, 2257, 3197
TaqI	TCGA	6 cut(s)	160, 372, 385, 1143, 2835, 3225
TatI	WGTACW	6 cut(s)	195, 705, 1194, 1701, 1780, 3054
TauI	GCSGC	2 cut(s)	3409, 3424
TfiI	GAWTC	3 cut(s)	140, 157, 469
Tru1I	TTAA	8 cut(s)	423, 602, 1152, 1871, 1950, 2199, 2714, 2948
Tru9I	TTAA	8 cut(s)	423, 602, 1152, 1871, 1950, 2199, 2714, 2948
TscAI	CASTG	6 cut(s)	277, 322, 2454, 2790, 2820, 2964
TseFI	GTSAC	3 cut(s)	268, 2452, 3186
TseI	GCWGC	7 cut(s)	28, 739, 1070, 1589, 1604, 2632, 3455
Tsp45I	GTSAC	3 cut(s)	268, 2452, 3186
TspGWI	ACGGA	3 cut(s)	291, 825, 1759
TspRI	CASTG	6 cut(s)	277, 322, 2454, 2790, 2820, 2964
Tth111I	GACNNNGTC	1 cut(s)	1808
VpaK11BI	GGWCC	2 cut(s)	688, 3462
XapI	RAATTY	8 cut(s)	509, 700, 1038, 1493, 1625, 1724, 1958, 2179
XbaI	TCTAGA	1 cut(s)	2386
XceI	RCATGY	3 cut(s)	1226, 1302, 3063
XmnI	GAANNNNTTC	4 cut(s)	509, 513, 1393, 1496
XspI	CTAG	4 cut(s)	2387, 2457, 3041, 3459
ZrmI	AGTACT	1 cut(s)	1782
Zsp2I	ATGCAT	3 cut(s)	2326, 3065, 3211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.