Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

62518315 .. 62524272

Gene Structure 5958 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00269750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3588 bp

ATGGGTGGTGGTGGTAGAAAGAAGAGGCAGCATTTTGGGAGAATCCATGCCTTTCACCGTGGAAAACCATCCGTAGATGAGGAGCATTCGCGGATCGGAGGCCCCGGATTCTCTAGAGTAGTCCATTGCAATGACCCTGAATGCCTTGAGGCCACTGCTAAGAACTATGGAAGCAATTATGTTAGAACCACCAAGTATACACTTGCTACATTCTTACCCAAGGCAGTGTTTGAGCAATTCAGAAGGGTTGCAAATCTGTATTTCCTCATTTGTGCAATACTGTCATTTACACCGCTTTCGCCTTACTCTGCTGTCAGTAATGTTGTCCCTCTTGTGGTTGTGGTTGGAGTTACAATGGGAAAAGAGGCCCTTGAAGATTGGAGGCGCAAAAAGCAGGATATGGATGTGAACAACAGAAAAGTTCTGGTGCATCGATCGGAAGGTGAATTTGATTTTACCAAGTGGAGGGATTTGAAAGTTGGGGATATAGTGAAAGTGGAAAAGGACCAATTCTTTCCTGCTGATCTTATCTTGCTTTCATCAAGCTATGATGAAGCACTTTGCTATGTAGAGACCACCAACCTTGATGGGGAAACTAATTTGAAAATAAAACAATCTCTGGAAGTAACTTCTAATCTTCATGAAGACTCGAGCTTCAAAGATTTCAAGGCTTTGATCAGATGTGAAGACCCGAATGCAAGTCTGTACTCATTCGTAGGGAGTCTGGAGTTTGCAGGGGAATCGTACCCTCTTTCACCTCAGCAGCTTCTGCTTAGGGACTCTAAGCTGAGAAACACTGATTTTATTTATGGGGTTGTAATCTTCACAGGTCATGATACAAAAGTTATGCAAAACTCAACTGAACCACCTTCCAAGAGAAGCAAAATTGAGAAAAGGATGGATAAGATAGTCTACTTCTTGTTTTTCCTCTTAGTTGTGATGTCTTTTGTTGGTGCTATTGTCTTTGGGGTTACCACTAGCGAAGACATAGAAGATGGGCAATTGATAAGATGGTACCTTAGACCAGATGATACTACAGTTTACTATGATCCAACAAGAGCGCCACTTGCAGCCATTTTGCAGTTCTTAACAGCCGTTATGTTGTACAGTTATTTGATTCCCATTTCCTTGTATGTGTCGATAGAGATTGTCAAAGTGCTCCAGAGCATTTTCATCAACCGGGATCTGCACATGTACTATGAGGAAACTGACAAGCCAGCTCGAGCACGTACCTCAAACTTGAATGAAGAACTTGGCCAAGTTGATACTATTCTGTCTGACAAAACAGGAACCTTAACGTGCAACTCAATGGAGTTTATCAAGTGTTCTATTGCCGGGACTGCTTTTGGGCGCGGAGTTACAGAAGTTGAGAGAGCTTTGTCCAGTGGCAAAGGCTCATCTTTGTTTGAAGGGGTGACAGAAGAAGGCCAAGTTGAAGAATCTACTGAAGCCAAGACATCAATAAAAGGCTTTAACTTCATGGACGAAAGAATAATGGATGGCAATTGGGTTAAGGAGCCTCGTGCAGATGTAATCCAGAAGTTTCTACAGTTACTCGCCGTCTGTCATACAGCAATACCTGAAGTTGATGAAGAATCTGGAAGAGTTTCATATGAGGCTGAATCACCAGATGAGGCAGCTTTTGTGATTGCAGCAAGAGAGCTTGGTTTTGAATTTTATGAAAGGGCTCAAACAAGCATCTCCCTGCACGAGTTTGATCCAATGTCTGGAAGGAGAGTTGAAAGATCTTATAAGATTTTGAATGTATTGGAGTTTAGTAGCTCAAGGAAGCGAATGTCTGTGATAGTAAGAAATGAGGAGGGAAAGATACTATTGCTCAGTAAAGGTGCTGACAGTGTCATGTTCCAAAGACTTGCAATGGATGGAAGGGAGTTTGAAGAGAAGACCCGGGAGCACATTAATGAGTATGCTGATGCTGGTTTGAGAACTTTAGTACTTGCATATCGGGAACTTGATGAAGAAGAGTATTTTGAATTTAACAAAGAATATACTGATGCCAAGAACTTGGTTAGTTCAGATCGAGAGGAAGCTGTCGAGAAAGTGGCTGAGAAGATAGAGAGGAACTTGATTCTTCTTGGTGCTACTGCAGTTGAAGACAAACTTCAAAATGGGGTTCCTGACTGCATTGATAAGCTTGCTCAGGCTGGAATTAAACTATGGGTTTTGACTGGAGATAAAATGGAGACAGCAATTAATATTGGATATGCCTGCAGTTTGCTTCGACAAGGAATGAAACAAATAATCATAAGCTCAGAGACCGCAGAAGCTAAAGCGCTAGAGAAGGTGGAGGACAAGTCCAAAGTTGCCATGGCATTGAAGGAAAGTGTTCTCCATCAAATAAGTGAGGCAAAGGCATTTCTTGCTTCACCAGATCAGAACTCCGAAGCATTGGCTTTGATCATTGATGGGAATTCACTCACTTATGCCTTAGAGGTAGATGTCCAGGACCTCTTTCTAGAGCTTGCCATTGGCTGTGCATCTGTTATATGCTGTCGTTCATCTCCCAAACAGAAAGCACTTGTCACAAGACTGGTAAAAGTTAAAACTGGTAAAACAACTCTGGCAATTGGTGATGGGGCAAATGATGTAGGAATGCTTCAAGAAGCAGATATTGGTGTAGGTATCAGCGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGTGACATTGCAATTGCACAATTTCGTTTTCTGGAGCGCCTGCTACTCGTACATGGACATTGGTGTTACAGAAGAATCTCGTCAATGATATGCTATTTCTTTTACAAGAACATTACTTTTGGCTTCACTATCTTTTTCTATGAGATGTATGCTTCATTCTCGGGTCAATCTGCATATAATGATTGGTATCTATCACTCTATAACGTCTTCTTCACATCACTTCCTGTAATTGCATTGGGAGTGTTTGACCAAGATGTCTCAGCCAGATTCTGCCTCAAGTTCCCACTCCTGTACCAAGAAGGTGCACAAAATGTCTTATTTAGCTGGGTCCGGATACTTGGATGGTCGCTGAACGGCATAGTGTCTGCTATGATAATCTTCTTCTGCTGCATCCTGGCAATAGGTAGTCAAGCCTTTCGCAAAGGCGGGGAAGTTGTTGGCTTCGAAATCTTTGGTGCCACAATGTACTCAATTGTGGTATGGGTTGTCAACTGTCAGATGGCACTATCCATCAGTTACTTCACTTACATACAACATCTCTTCATCTGGGGTGGCATTGTGTTCTGGTACATATTCCAGCTGGCATATGGAGCCTTGGACCCTTCCATATCAACCACTGCCTATAAGGTCTTCATTGAAGCCTGTGCACCAGCCCCATTTTATTGGCTTCTCACACTCTTGGTAGTAGTCACTTCTCTCCTACCTTATTTCACTTACGCTGCCTTCCAAATGCGGTTCTTCCCCATGTATCATCAGATGATACAATGGATGAGAACAGATGGGCAATCAGATGACCCTGAGTTCTGTCGTATGGTGAGGCAGAGATCGATAAGACCCACAACAGTTGGCTACACAGCTCGCATTGCAACGTCTAAACGCTTTGAAGAGAAGCCCGAGAGTAGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1195

Amino Acids

134.97

Weight (kDa)

5.47

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 106	7.6e-24	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	513 - 709	4.8e-21	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	891 - 1141	4e-87	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1752
AasI	GACNNNNNNGTC	1 cut(s)	2933
Acc65I	GGTACC	1 cut(s)	1014
AccB1I	GGYRCC	2 cut(s)	1014, 3134
AccB7I	CCANNNNNTGG	1 cut(s)	1727
AccI	GTMKAC	2 cut(s)	197, 912
AccII	CGCG	2 cut(s)	91, 1353
AccIII	TCCGGA	1 cut(s)	3009
AciI	CCGC	7 cut(s)	91, 293, 1353, 2280, 2649, 3105, 3412
AclWI	GGATC	4 cut(s)	101, 1043, 1191, 1712
AcoI	YGGCCR	1 cut(s)	1255
AcsI	RAATTY	4 cut(s)	446, 1673, 1994, 2431
AcuI	CTGAAG	2 cut(s)	1467, 1602
AfeI	AGCGCT	1 cut(s)	2295
AfiI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1727
AflIII	ACRYGT	1 cut(s)	1191
AjnI	CCWGG	2 cut(s)	2463, 3072
AjuI	GAANNNNNNNTTGG	2 cut(s)	2616, 2648
Alw21I	GWGCWC	5 cut(s)	1161, 1228, 1917, 2986, 3328
Alw26I	GTCTC	4 cut(s)	566, 2198, 2270, 2941
Alw44I	GTGCAC	2 cut(s)	2982, 3324
AlwI	GGATC	4 cut(s)	101, 1043, 1191, 1712
AlwNI	CAGNNNCTG	2 cut(s)	769, 2949
Ama87I	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
Aor13HI	TCCGGA	1 cut(s)	3009
Aor51HI	AGCGCT	1 cut(s)	2295
AoxI	GGCC	5 cut(s)	100, 150, 366, 1255, 1426
ApaLI	GTGCAC	2 cut(s)	2982, 3324
ApeKI	GCWGC	7 cut(s)	28, 763, 1070, 1637, 1652, 3066, 3398
ApoI	RAATTY	4 cut(s)	446, 1673, 1994, 2431
AseI	ATTAAT	2 cut(s)	1920, 2214
Asp700I	GAANNNNTTC	2 cut(s)	1606, 2346
Asp718I	GGTACC	1 cut(s)	1014
AspLEI	GCGC	5 cut(s)	387, 1063, 1353, 2296, 2718
AspS9I	GGNCC	6 cut(s)	101, 367, 505, 2467, 3007, 3277
AsuC2I	CCSGG	5 cut(s)	105, 1181, 1336, 1909, 1910
AsuHPI	GGTGA	8 cut(s)	47, 455, 747, 1426, 1617, 2379, 2603, 3505
AsuII	TTCGAA	1 cut(s)	3123
AvaI	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
AvaII	GGWCC	4 cut(s)	505, 2467, 3007, 3277
BaeGI	GKGCMC	2 cut(s)	2986, 3328
BaeI	ACNNNNGTAYC	6 cut(s)	828, 861, 1023, 1056, 2954, 2987
BalI	TGGCCA	1 cut(s)	1257
BanI	GGYRCC	2 cut(s)	1014, 3134
BanII	GRGCYC	1 cut(s)	1690
BarI	GAAGNNNNNNTAC	2 cut(s)	2601, 2633
BauI	CACGAG	2 cut(s)	1521, 1709
BbsI	GAAGAC	7 cut(s)	651, 693, 990, 1910, 2121, 2878, 3301
Bbv12I	GWGCWC	5 cut(s)	1161, 1228, 1917, 2986, 3328
BbvCI	CCTCAGC	1 cut(s)	759
BbvI	GCAGC	7 cut(s)	40, 775, 1082, 1649, 1664, 3053, 3385
BceAI	ACGGC	3 cut(s)	1079, 1544, 3049
BcgI	CGANNNNNNTGC	2 cut(s)	723, 757
BciT130I	CCWGG	2 cut(s)	2465, 3074
BciVI	GTATCC	1 cut(s)	3006
BclI	TGATCA	2 cut(s)	675, 2418
BcnI	CCSGG	5 cut(s)	105, 1181, 1336, 1909, 1910
BcoDI	GTCTC	4 cut(s)	566, 2198, 2270, 2941
BfaI	CTAG	4 cut(s)	114, 978, 2297, 2477
BfmI	CTRYAG	4 cut(s)	1035, 1547, 2106, 2230
BfoI	RGCGCY	3 cut(s)	1064, 2297, 2719
BfuI	GTATCC	1 cut(s)	3006
BglII	AGATCT	1 cut(s)	1745
BisI	GCNGC	7 cut(s)	29, 764, 1071, 1638, 1653, 3067, 3399
BlsI	GCNGC	7 cut(s)	30, 765, 1072, 1639, 1654, 3068, 3400
BmcAI	AGTACT	1 cut(s)	1956
Bme1390I	CCNGG	7 cut(s)	105, 1181, 1336, 1909, 1910, 2465, 3074
Bme18I	GGWCC	4 cut(s)	505, 2467, 3007, 3277
BmeT110I	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
BmgT120I	GGNCC	6 cut(s)	101, 367, 505, 2467, 3007, 3277
BmiI	GGNNCC	9 cut(s)	103, 1016, 1291, 1518, 2136, 3008, 3136, 3271, 3279
BmrFI	CCNGG	7 cut(s)	105, 1181, 1336, 1909, 1910, 2465, 3074
BmsI	GCATC	6 cut(s)	439, 1707, 1924, 2005, 2507, 3078
BpiI	GAAGAC	7 cut(s)	651, 693, 990, 1910, 2121, 2878, 3301
BpmI	CTGGAG	4 cut(s)	746, 1145, 2211, 2732
Bpu10I	CCTNAGC	3 cut(s)	759, 773, 2160
Bpu14I	TTCGAA	1 cut(s)	3123
BpuEI	CTTGAG	3 cut(s)	167, 1768, 2939
BpuMI	CCSGG	5 cut(s)	105, 1181, 1336, 1909, 1910
Bsa29I	ATCGAT	2 cut(s)	433, 3506
BsaAI	YACGTR	1 cut(s)	1229
BsaBI	GATNNNNATC	1 cut(s)	2865
BsaI	GGTCTC	2 cut(s)	566, 2270
BsaJI	CCNNGG	6 cut(s)	58, 103, 219, 1908, 2328, 3273
BsaWI	WCCGGW	1 cut(s)	3009
BsaXI	ACNNNNNCTCC	2 cut(s)	2300, 2330
Bsc4I	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1727
Bse1I	ACTGG	4 cut(s)	1383, 2194, 2556, 2572
Bse3DI	GCAATG	5 cut(s)	124, 136, 1884, 2685, 3540
Bse8I	GATNNNNATC	1 cut(s)	2865
BseAI	TCCGGA	1 cut(s)	3009
BseBI	CCWGG	2 cut(s)	2465, 3074
BseCI	ATCGAT	2 cut(s)	433, 3506
BseDI	CCNNGG	6 cut(s)	58, 103, 219, 1908, 2328, 3273
BseGI	GGATG	8 cut(s)	68, 409, 903, 1504, 1888, 3026, 3069, 3453
BseJI	GATNNNNATC	1 cut(s)	2865
BseLI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1727
BseMI	GCAATG	5 cut(s)	124, 136, 1884, 2685, 3540
BseMII	CTCAG	8 cut(s)	773, 779, 1852, 2058, 2174, 2286, 2952, 3468
BseNI	ACTGG	4 cut(s)	1383, 2194, 2556, 2572
BseRI	GAGGAG	2 cut(s)	95, 1832
BseSI	GKGCMC	2 cut(s)	2986, 3328
BseXI	GCAGC	7 cut(s)	40, 775, 1082, 1649, 1664, 3053, 3385
BseYI	CCCAGC	1 cut(s)	3003
BsgI	GTGCAG	3 cut(s)	1172, 1545, 1691
Bsh1236I	CGCG	2 cut(s)	91, 1353
Bsh1285I	CGRYCG	1 cut(s)	437
BshFI	GGCC	5 cut(s)	102, 152, 368, 1257, 1428
BshNI	GGYRCC	2 cut(s)	1014, 3134
BshVI	ATCGAT	2 cut(s)	433, 3506
BsiEI	CGRYCG	1 cut(s)	437
BsiHKAI	GWGCWC	5 cut(s)	1161, 1228, 1917, 2986, 3328
BsiHKCI	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
BsiSI	CCGG	5 cut(s)	105, 1180, 1335, 1909, 3010
BslFI	GGGAC	3 cut(s)	311, 791, 1351
BslI	CCNNNNNNNGG	4 cut(s)	334, 465, 589, 1727
BsmAI	GTCTC	4 cut(s)	566, 2198, 2270, 2941
BsmFI	GGGAC	3 cut(s)	311, 791, 1351
BsmI	GAATGC	5 cut(s)	85, 146, 700, 2619, 2667
BsnI	GGCC	5 cut(s)	102, 152, 368, 1257, 1428
Bso31I	GGTCTC	2 cut(s)	566, 2270
BsoBI	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
Bsp119I	TTCGAA	1 cut(s)	3123
Bsp1286I	GDGCHC	6 cut(s)	1161, 1228, 1690, 1917, 2986, 3328
Bsp13I	TCCGGA	1 cut(s)	3009
Bsp1407I	TGTACA	1 cut(s)	1104
Bsp19I	CCATGG	1 cut(s)	2328
BspACI	CCGC	7 cut(s)	91, 293, 1353, 2280, 2649, 3105, 3412
BspANI	GGCC	5 cut(s)	102, 152, 368, 1257, 1428
BspCNI	CTCAG	8 cut(s)	772, 780, 1851, 2059, 2173, 2285, 2951, 3469
BspDI	ATCGAT	2 cut(s)	433, 3506
BspEI	TCCGGA	1 cut(s)	3009
BspFNI	CGCG	2 cut(s)	91, 1353
BspHI	TCATGA	2 cut(s)	640, 832
BspLI	GGNNCC	9 cut(s)	103, 1016, 1291, 1518, 2136, 3008, 3136, 3271, 3279
BspMAI	CTGCAG	2 cut(s)	2110, 2234
BspPI	GGATC	4 cut(s)	101, 1043, 1191, 1712
BspT104I	TTCGAA	1 cut(s)	3123
BspT107I	GGYRCC	2 cut(s)	1014, 3134
BspTNI	GGTCTC	2 cut(s)	566, 2270
BsrDI	GCAATG	5 cut(s)	124, 136, 1884, 2685, 3540
BsrGI	TGTACA	1 cut(s)	1104
BsrI	ACTGG	4 cut(s)	1383, 2194, 2556, 2572
BssECI	CCNNGG	6 cut(s)	58, 103, 219, 1908, 2328, 3273
BssNAI	GTATAC	1 cut(s)	198
BssSI	CACGAG	2 cut(s)	1521, 1709
BssT1I	CCWWGG	3 cut(s)	219, 2328, 3273
Bst1107I	GTATAC	1 cut(s)	198
Bst2BI	CACGAG	2 cut(s)	1521, 1709
Bst2UI	CCWGG	2 cut(s)	2465, 3074
Bst4CI	ACNGT	8 cut(s)	59, 282, 1039, 1109, 1551, 1856, 3173, 3523
Bst6I	CTCTTC	6 cut(s)	17, 1597, 1893, 1977, 3224, 3558
BstAPI	GCANNNNNTGC	2 cut(s)	769, 2381
BstAUI	TGTACA	1 cut(s)	1104
BstBAI	YACGTR	1 cut(s)	1229
BstBI	TTCGAA	1 cut(s)	3123
BstC8I	GCNNGC	8 cut(s)	1218, 2157, 2230, 2484, 2667, 2720, 3261, 3538
BstDSI	CCRYGG	2 cut(s)	58, 2328
BstEII	GGTNACC	1 cut(s)	970
BstF5I	GGATG	8 cut(s)	68, 409, 903, 1504, 1888, 3026, 3069, 3453
BstFNI	CGCG	2 cut(s)	91, 1353
BstH2I	RGCGCY	3 cut(s)	1064, 2297, 2719
BstHHI	GCGC	5 cut(s)	387, 1063, 1353, 2296, 2718
BstMAI	GTCTC	4 cut(s)	566, 2198, 2270, 2941
BstMCI	CGRYCG	1 cut(s)	437
BstMWI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2381, 3269, 3542
BstNI	CCWGG	2 cut(s)	2465, 3074
BstNSI	RCATGY	1 cut(s)	1195
BstPI	GGTNACC	1 cut(s)	970
BstSCI	CCNGG	7 cut(s)	103, 1179, 1334, 1907, 1908, 2463, 3072
BstSFI	CTRYAG	4 cut(s)	1035, 1547, 2106, 2230
BstSLI	GKGCMC	2 cut(s)	2986, 3328
BstUI	CGCG	2 cut(s)	91, 1353
BstV1I	GCAGC	7 cut(s)	40, 775, 1082, 1649, 1664, 3053, 3385
BstV2I	GAAGAC	7 cut(s)	651, 693, 990, 1910, 2121, 2878, 3301
BstX2I	RGATCY	2 cut(s)	1183, 1745
BstXI	CCANNNNNNTGG	2 cut(s)	2026, 3342
BstYI	RGATCY	2 cut(s)	1183, 1745
BstZ17I	GTATAC	1 cut(s)	198
Bsu15I	ATCGAT	2 cut(s)	433, 3506
BsuI	GTATCC	1 cut(s)	3006
BsuRI	GGCC	5 cut(s)	102, 152, 368, 1257, 1428
BsuTUI	ATCGAT	2 cut(s)	433, 3506
BtgI	CCRYGG	2 cut(s)	58, 2328
BtsCI	GGATG	8 cut(s)	68, 409, 903, 1504, 1888, 3026, 3069, 3453
BtsI	GCAGTG	3 cut(s)	153, 231, 3294
BtsIMutI	CAGTG	6 cut(s)	153, 231, 795, 1390, 1861, 3294
Cac8I	GCNNGC	8 cut(s)	1218, 2157, 2230, 2484, 2667, 2720, 3261, 3538
CaiI	CAGNNNCTG	2 cut(s)	769, 2949
CciI	TCATGA	2 cut(s)	640, 832
CfoI	GCGC	5 cut(s)	387, 1063, 1353, 2296, 2718
Cfr13I	GGNCC	6 cut(s)	101, 367, 505, 2467, 3007, 3277
Cfr9I	CCCGGG	1 cut(s)	1908
ClaI	ATCGAT	2 cut(s)	433, 3506
CspCI	CAANNNNNGTGG	4 cut(s)	565, 600, 3505, 3540
DrdI	GACNNNNNNGTC	1 cut(s)	2933
DseDI	GACNNNNNNGTC	1 cut(s)	2933
EaeI	YGGCCR	1 cut(s)	1255
Eam1104I	CTCTTC	6 cut(s)	17, 1597, 1893, 1977, 3224, 3558
EarI	CTCTTC	6 cut(s)	17, 1597, 1893, 1977, 3224, 3558
Eco130I	CCWWGG	3 cut(s)	219, 2328, 3273
Eco24I	GRGCYC	1 cut(s)	1690
Eco31I	GGTCTC	2 cut(s)	566, 2270
Eco47I	GGWCC	4 cut(s)	505, 2467, 3007, 3277
Eco47III	AGCGCT	1 cut(s)	2295
Eco57I	CTGAAG	2 cut(s)	1467, 1602
Eco88I	CYCGRG	5 cut(s)	649, 1221, 1908, 2839, 3572
Eco91I	GGTNACC	1 cut(s)	970
EcoO109I	RGGNCCY	3 cut(s)	101, 367, 2467
EcoO65I	GGTNACC	1 cut(s)	970
EcoRI	GAATTC	1 cut(s)	2431
EcoRII	CCWGG	2 cut(s)	2463, 3072
EcoT14I	CCWWGG	3 cut(s)	219, 2328, 3273
EcoT38I	GRGCYC	1 cut(s)	1690
ErhI	CCWWGG	3 cut(s)	219, 2328, 3273
FalI	AAGNNNNNCTT	4 cut(s)	354, 386, 2106, 2138
FaqI	GGGAC	3 cut(s)	311, 791, 1351
FauI	CCCGC	1 cut(s)	3098
FauNDI	CATATG	2 cut(s)	1612, 3265
FbaI	TGATCA	2 cut(s)	675, 2418
FblI	GTMKAC	2 cut(s)	197, 912
Fnu4HI	GCNGC	7 cut(s)	29, 764, 1071, 1638, 1653, 3067, 3399
FokI	GGATG	8 cut(s)	55, 416, 910, 1511, 1895, 3033, 3056, 3460
FriOI	GRGCYC	1 cut(s)	1690
Fsp4HI	GCNGC	7 cut(s)	29, 764, 1071, 1638, 1653, 3067, 3399
FspBI	CTAG	4 cut(s)	114, 978, 2297, 2477
GlaI	GCGC	5 cut(s)	386, 1062, 1352, 2295, 2717
GluI	GCNGC	7 cut(s)	29, 764, 1071, 1638, 1653, 3067, 3399
GsaI	CCCAGC	1 cut(s)	3007
GsuI	CTGGAG	4 cut(s)	746, 1145, 2211, 2732
HaeII	RGCGCY	3 cut(s)	1064, 2297, 2719
HaeIII	GGCC	5 cut(s)	102, 152, 368, 1257, 1428
HapII	CCGG	5 cut(s)	105, 1180, 1335, 1909, 3010
HhaI	GCGC	5 cut(s)	387, 1063, 1353, 2296, 2718
Hin6I	GCGC	5 cut(s)	385, 1061, 1351, 2294, 2716
HinP1I	GCGC	5 cut(s)	385, 1061, 1351, 2294, 2716
HincII	GTYRAC	1 cut(s)	3169
HindII	GTYRAC	1 cut(s)	3169
HindIII	AAGCTT	1 cut(s)	2153
HpaII	CCGG	5 cut(s)	105, 1180, 1335, 1909, 3010
HphI	GGTGA	8 cut(s)	47, 455, 747, 1426, 1617, 2379, 2603, 3505
Hpy166II	GTNNAC	7 cut(s)	198, 409, 913, 1042, 2984, 3169, 3326
Hpy8I	GTNNAC	7 cut(s)	198, 409, 913, 1042, 2984, 3169, 3326
HpyCH4III	ACNGT	8 cut(s)	59, 282, 1039, 1109, 1551, 1856, 3173, 3523
HpyCH4IV	ACGT	4 cut(s)	1228, 1298, 2883, 3548
HpyF10VI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2381, 3269, 3542
HpySE526I	ACGT	4 cut(s)	1228, 1298, 2883, 3548
HspAI	GCGC	5 cut(s)	385, 1061, 1351, 2294, 2716
Kpn2I	TCCGGA	1 cut(s)	3009
KpnI	GGTACC	1 cut(s)	1018
Ksp22I	TGATCA	2 cut(s)	675, 2418
LmnI	GCTCC	6 cut(s)	82, 1164, 1516, 1912, 2713, 3269
Lsp1109I	GCAGC	7 cut(s)	40, 775, 1082, 1649, 1664, 3053, 3385
LweI	GCATC	6 cut(s)	439, 1707, 1924, 2005, 2507, 3078
MaeI	CTAG	4 cut(s)	114, 978, 2297, 2477
MaeII	ACGT	4 cut(s)	1228, 1298, 2883, 3548
MfeI	CAATTG	5 cut(s)	1001, 1504, 2586, 2691, 3150
MflI	RGATCY	2 cut(s)	1183, 1745
MhlI	GDGCHC	6 cut(s)	1161, 1228, 1690, 1917, 2986, 3328
MlsI	TGGCCA	1 cut(s)	1257
MluNI	TGGCCA	1 cut(s)	1257
MlyI	GAGTC	3 cut(s)	641, 730, 773
MmeI	TCCRAC	2 cut(s)	325, 1076
Mox20I	TGGCCA	1 cut(s)	1257
MroI	TCCGGA	1 cut(s)	3009
MroXI	GAANNNNTTC	2 cut(s)	1606, 2346
MscI	TGGCCA	1 cut(s)	1257
MseI	TTAA	9 cut(s)	1088, 1295, 1473, 1512, 1920, 1998, 2172, 2214, 2562
MslI	CAYNNNNRTG	2 cut(s)	129, 1920
Msp20I	TGGCCA	1 cut(s)	1257
MspA1I	CMGCKG	2 cut(s)	2649, 3259
MspI	CCGG	5 cut(s)	105, 1180, 1335, 1909, 3010
MspR9I	CCNGG	7 cut(s)	105, 1181, 1336, 1909, 1910, 2465, 3074
MunI	CAATTG	5 cut(s)	1001, 1504, 2586, 2691, 3150
Mva1269I	GAATGC	5 cut(s)	85, 146, 700, 2619, 2667
MvaI	CCWGG	2 cut(s)	2465, 3074
MvnI	CGCG	2 cut(s)	91, 1353
MwoI	GCNNNNNNNGC	7 cut(s)	391, 769, 1067, 1340, 2381, 3269, 3542
NciI	CCSGG	5 cut(s)	105, 1181, 1336, 1909, 1910
NcoI	CCATGG	1 cut(s)	2328
NdeI	CATATG	2 cut(s)	1612, 3265
NlaIV	GGNNCC	9 cut(s)	103, 1016, 1291, 1518, 2136, 3008, 3136, 3271, 3279
NmuCI	GTSAC	4 cut(s)	1414, 2542, 2681, 3367
NspI	RCATGY	1 cut(s)	1195
NspV	TTCGAA	1 cut(s)	3123
PaeR7I	CTCGAG	2 cut(s)	649, 1221
PagI	TCATGA	2 cut(s)	640, 832
PciI	ACATGT	1 cut(s)	1191
PctI	GAATGC	5 cut(s)	85, 146, 700, 2619, 2667
PdmI	GAANNNNTTC	2 cut(s)	1606, 2346
PflFI	GACNNNGTC	1 cut(s)	1856
PflMI	CCANNNNNTGG	1 cut(s)	1727
PfoI	TCCNGGA	1 cut(s)	2463
PkrI	GCNGC	7 cut(s)	30, 765, 1072, 1639, 1654, 3068, 3400
Ple19I	CGATCG	1 cut(s)	437
PleI	GAGTC	3 cut(s)	641, 729, 773
PpsI	GAGTC	3 cut(s)	641, 729, 773
Ppu21I	YACGTR	1 cut(s)	1229
PpuMI	RGGWCCY	1 cut(s)	2467
PscI	ACATGT	1 cut(s)	1191
PshBI	ATTAAT	2 cut(s)	1920, 2214
PsiI	TTATAA	1 cut(s)	1752
Psp5II	RGGWCCY	1 cut(s)	2467
Psp6I	CCWGG	2 cut(s)	2463, 3072
PspEI	GGTNACC	1 cut(s)	970
PspFI	CCCAGC	1 cut(s)	3003
PspGI	CCWGG	2 cut(s)	2463, 3072
PspN4I	GGNNCC	9 cut(s)	103, 1016, 1291, 1518, 2136, 3008, 3136, 3271, 3279
PspPI	GGNCC	6 cut(s)	101, 367, 505, 2467, 3007, 3277
PspPPI	RGGWCCY	1 cut(s)	2467
PspXI	VCTCGAGB	2 cut(s)	649, 1221
PstI	CTGCAG	2 cut(s)	2110, 2234
PstNI	CAGNNNCTG	2 cut(s)	769, 2949
PsuI	RGATCY	2 cut(s)	1183, 1745
PsyI	GACNNNGTC	1 cut(s)	1856
PvuI	CGATCG	1 cut(s)	437
PvuII	CAGCTG	1 cut(s)	3259
RseI	CAYNNNNRTG	2 cut(s)	129, 1920
SaqAI	TTAA	9 cut(s)	1088, 1295, 1473, 1512, 1920, 1998, 2172, 2214, 2562
SatI	GCNGC	7 cut(s)	29, 764, 1071, 1638, 1653, 3067, 3399
Sau96I	GGNCC	6 cut(s)	101, 367, 505, 2467, 3007, 3277
ScaI	AGTACT	1 cut(s)	1956
SchI	GAGTC	3 cut(s)	641, 730, 773
ScrFI	CCNGG	7 cut(s)	105, 1181, 1336, 1909, 1910, 2465, 3074
SduI	GDGCHC	6 cut(s)	1161, 1228, 1690, 1917, 2986, 3328
SfaNI	GCATC	6 cut(s)	439, 1707, 1924, 2005, 2507, 3078
SfcI	CTRYAG	4 cut(s)	1035, 1547, 2106, 2230
Sfr274I	CTCGAG	2 cut(s)	649, 1221
SfuI	TTCGAA	1 cut(s)	3123
SinI	GGWCC	4 cut(s)	505, 2467, 3007, 3277
SlaI	CTCGAG	2 cut(s)	649, 1221
SmaI	CCCGGG	1 cut(s)	1910
SmiMI	CAYNNNNRTG	2 cut(s)	129, 1920
SmlI	CTYRAG	5 cut(s)	146, 649, 1221, 1783, 2954
SmoI	CTYRAG	5 cut(s)	146, 649, 1221, 1783, 2954
SsiI	CCGC	7 cut(s)	91, 293, 1353, 2280, 2649, 3105, 3412
SspI	AATATT	1 cut(s)	2218
SspMI	CTAG	4 cut(s)	114, 978, 2297, 2477
StyD4I	CCNGG	7 cut(s)	103, 1179, 1334, 1907, 1908, 2463, 3072
StyI	CCWWGG	3 cut(s)	219, 2328, 3273
TaaI	ACNGT	8 cut(s)	59, 282, 1039, 1109, 1551, 1856, 3173, 3523
TaiI	ACGT	4 cut(s)	1231, 1301, 2886, 3551
TaqI	TCGA	9 cut(s)	433, 650, 1139, 1222, 2041, 2055, 2242, 3123, 3506
TatI	WGTACW	5 cut(s)	705, 1104, 1194, 1954, 3144
Tru1I	TTAA	9 cut(s)	1088, 1295, 1473, 1512, 1920, 1998, 2172, 2214, 2562
Tru9I	TTAA	9 cut(s)	1088, 1295, 1473, 1512, 1920, 1998, 2172, 2214, 2562
TscAI	CASTG	6 cut(s)	160, 231, 802, 1390, 1861, 3301
TseFI	GTSAC	4 cut(s)	1414, 2542, 2681, 3367
TseI	GCWGC	7 cut(s)	28, 763, 1070, 1637, 1652, 3066, 3398
Tsp45I	GTSAC	4 cut(s)	1414, 2542, 2681, 3367
TspGWI	ACGGA	1 cut(s)	61
TspMI	CCCGGG	1 cut(s)	1908
TspRI	CASTG	6 cut(s)	160, 231, 802, 1390, 1861, 3301
Tth111I	GACNNNGTC	1 cut(s)	1856
Van91I	CCANNNNNTGG	1 cut(s)	1727
VneI	GTGCAC	2 cut(s)	2982, 3324
VpaK11BI	GGWCC	4 cut(s)	505, 2467, 3007, 3277
VspI	ATTAAT	2 cut(s)	1920, 2214
XapI	RAATTY	4 cut(s)	446, 1673, 1994, 2431
XbaI	TCTAGA	2 cut(s)	113, 2476
XceI	RCATGY	1 cut(s)	1195
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2326, 3263
XhoI	CTCGAG	2 cut(s)	649, 1221
XmaI	CCCGGG	1 cut(s)	1908
XmiI	GTMKAC	2 cut(s)	197, 912
XmnI	GAANNNNTTC	2 cut(s)	1606, 2346
XspI	CTAG	4 cut(s)	114, 978, 2297, 2477
ZrmI	AGTACT	1 cut(s)	1956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.