Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Forward (+)

3657524 .. 3663161

Gene Structure 5638 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1051100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3594 bp

ATGCGGGGTGGAGGCAGAAGGAGAAAGCTGCATTTCAGCAAGATCTATTCTTTCACTTGTGGTAAATCTTCTATGAGAGATGAACATTCACAAATTGGGGGACCAGGATATTCCAGAGTGGTTTACTGCAATGAACCGGACTCTTTCGAGGCGCATATGCGAAATTATGGAGATAATTATGTTAGGAGCACAAAGTATACAGTTGCTACTTTCTTGCCAAAATCTTTGTTTGAGCAGTTCAGAAGGGTGGCCAACTTCTACTTCTTAGTCACTGGCACTTTGGCATTCACTCCTCTGGCTCCATTTACCGCTGTCAGCGCGATCATCCCTCTTATTATTGTGATTGGGGCGACCATGGTGAAAGAGGGTATCGAAGATTGGCGGCGAAAACAGCAGGATATTGAGGTGAACAATCGAAAGGTTAAAGTACATTATGGTAATGGGGTTTTCGATTATACTGCTTGGAGGAATCTGAGGGTGGGAGATATAGTGAGAGTAGAAAAAGATGAATTCTTTCCGACAGACCTCCTCTTGCTTTCATCCAGTTATGACGATGCAATCTGCTATGTTGAGACCATGAATCTTGATGGGGAGACAAATTTAAAGTTAAAACAAGCATTGGAAGTAACTTCGTTCTTACAGGAGGACTCTAACTTCAATGATTTTAATGCCATTGTTAAATGTGAAGACCCGAATGCAAATTTGTACTCGTTTGTCGGAACTATGGAGTTTGAAAAGCAACAGTTTCCCCTCTCTCCTCAACAGCTTCTCCTCAGAGACTCTAAACTCAGAAATACAGACTACATATATGGAGCTGTTATCTTCACTGGTCCCGACACAAAGGTTATTCAAAATTCTACTGCCCCTCCTTCAAAAAGAAGCAGAGTTGAGAAGAAGATGGATAAAATTATCTACTTCTTGTTCTGTGTTTTATTCATGATGGCATTTGTTGGGTCGATTTACTTTGGGATTGCAACGAAGGATGACCTAAACAATGGGATCATGAAAAGGTGGTATCTCAGGCCAGATAATTCTAGAATTTTCTTTGATGCTAAAAGAGCTCCGTATGCAGCAATTTACCACTTTTTGACAGCTCTAATGTTGTATGGCTACTTTATCCCCATCTCCTTGTATGTGTCGATAGAAATTGTCAAAGTTCTTCAGAGCATATTCATCAATCAAGATATTCACATGTACTATGAGGAAGCTGACAAACCAGCACTTGCCCGTACCTCAAACTTGAACGAGGAACTTGGCCAAGTTGACACGATTCTGTCTGATAAGACTGGAACTCTGACCTGCAATTCAATGGAGTTTATTAAATGTTCTGTGGCCGGAACAGCTTATGGCCAAGGATATACTGAGGTCGAGAGGGCTATGGGCAGGAGAAATGGTTCACCTTTGGTTCATCAACATTTAAGTGGTGGGGACAATCTCAAGGACACTACTGATACGAAGGCACCCATCAAGGGCTTTAATTTTAAAGACGAAAGGGTCATGAATGGAAACTGGGTTAATGAGCCTAATGCAGAATACATCCAGAAGTTTTTCAGTTTATTAGCAATCTGTCATACGGCAATTCCAGAAGTTGATGAAGCTAATGGGAATATTTCATATGAGGCTGAATCTCCTGATGAAGCAGCATTTGTGATTGCAGCAAGAGAACTTGGTTTTGAATTCTACAAAAGGACGCAAACAACCATATCACTGCGAGAGTTGGATCCAGTGTCCGGCAAGAAAGTTGAGAGGACGTATAGCCTTCTCAATGTTTTAGAGTTTAACAGCACAAGGAAGCGAATGTCTGTGATTGTCAGAAGTGAGGAAGGAAAAATACTATTACTTTCCAAAGGTGCCGACAATGTTATGCTTGAAAGGCTTGCCAGAAATTGTTCGGATTTTGAAGAAGAGACCATGGAGCATCTAAATGAGTATGCTGATGCAGGGCTGAGGACCTTGATTCTTGCCTACCGTGAACTTGAGGAAGATGAATACAAAGAGTTCAATCAAAATTTTATCAAGGCCAAGAATTCAGTTAGTGCAGAACGCGAAACAGTCATCGATGAAGTAACAGAAAAGATTGAGAGAGATCTAATTCTTCTTGGTGCAACTGCTGTTGAGGACAAACTACAAAATGGGGTTCCTGATTGCATTGACAAGCTTGCCCAAGCAGGAATTAAGATTTGGGTTTTGACTGGAGACAAGATGGAGACTGCCATCAATATCGGGTTTGCATGTAGCTTGCTTAGACAAGGAATGAAGCAAATTGTAATCACTTTGGAGTCTCCGGATATTAAAGCGTTGGAAAAGGAAGGAGAAAAGGAGGCCATCGCCACGGCATCAAAAGGAAGTGTTCTCAATCAGATAATTAGGGGAAAAGCTCAGCTTACAGCGTCGAGCAGGAACTCTGAGGCATTTGCATTGATCATTGATGGAAAATCACTTGCTTACGCTTTGGAGGATGATGTAAAGAATTTGTTTCTAAATCTTGCAATTGGCTGTGCATCTGTTATTTGCTGCCGTTCATCACCTAAACAGAAGGCACTGGTCACTAGACTGGTAAAATCTGGAACTGGGAAAACAACACTAGCGATCGGTGATGGAGCCAATGACGTGGGCATGCTCCAAGAAGCAGATATCGGGGTTGGAATTAGTGGTGTTGAAGGAATGCAGGCAGTCATGTCAAGCGATATTGCTATTGCACAGTTCCAATACTTGGAGCGTCTACTGCTTGTGCACGGGCATTGGTGTTACAGAAGGATCTCATCAATGATTTGCTACTTCTTCTACAAGAACATTGCATTTGGTTTCACTCTCTTCTTATATGAGGCACACACATCCTTCTCTGGAACGCCTGCATACAACGATTGGTTTTTGTCACTTTACAACGTATTCTTCTCTTCACTTCCGGTGGTTGCTATGGGAGTTTTCGACCAGGATGTATCCGCGCGGTTCTGTTTCAAGTTTCCTCTATTATACCAAGAAGGGGTGCAAAATATTCTCTTCAGCTGGCGTAGAATATTCGGATGGATGTTGAATGGGTTTACAACGGCAGCGATTATCTTCTTCTTCTGCACGAAAGCTCTAGCACACCAGGCCTTCAATAACGAGGGCAAAACTGCCGGAAGGGATATCCTCGGGGCAACGATGTACACGTGCACGGTTTGGGTTGTGAACTTGCAGATGGCACTTTCCATCAGTTACTTCACCTTGATACAGCACCTCTTCATCTGGGGTTCAATTGCACTATGGTATCTCTTCCTCTTGGCGTACGGCGCCATGTCACCTTCACTCTCCACCACTGCCTACAAAATCTTCATCGAAGCCCTCGCCCCCGCGCCTTCTTTCTGGCTCATCACAATCTTCGTGCCAGTCTCCGGTCTAATCCTATTCTTCACATACTCGGCCGTTCAAATGCGGTTCTTCCCTATGTACCATAGAACGATACAACGGATACGGTACGAGGGAACATCAAATGATCCCAAATTTTGTGACATGGTGCGGCAGAGATCGTTGCGGCCGCAGACTGTTGGTTTCACGGCGCGACTGGCGGCGAGGGCCAACCGTATAAAAGATAGACACCATAACCGTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1198

Amino Acids

135.49

Weight (kDa)

7.22

Isoelectric Point (pI)

36.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 108	2.6e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	517 - 710	6.4e-20	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	892 - 1142	3.4e-85	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1493
Acc36I	ACCTGC	1 cut(s)	1307
AccB1I	GGYRCC	3 cut(s)	1459, 1850, 3272
AccB7I	CCANNNNNTGG	1 cut(s)	2713
AccI	GTMKAC	2 cut(s)	197, 2722
AccII	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
AccIII	TCCGGA	1 cut(s)	2284
AclWI	GGATC	5 cut(s)	1007, 1715, 1728, 2765, 3470
AcoI	YGGCCR	6 cut(s)	249, 1255, 1332, 1348, 3402, 3515
AcuI	CTGAAG	2 cut(s)	1145, 2986
AcvI	CACGTG	1 cut(s)	3153
AcyI	GRCGYC	1 cut(s)	3273
AfaI	GTAC	8 cut(s)	429, 707, 1196, 1231, 3149, 3269, 3431, 3458
AfiI	CCNNNNNNNGG	5 cut(s)	1469, 1730, 2713, 2983, 3374
AflIII	ACRYGT	2 cut(s)	1191, 3150
AjiI	CACGTC	1 cut(s)	2611
AjnI	CCWGG	3 cut(s)	103, 2931, 3090
AjuI	GAANNNNNNNTTGG	4 cut(s)	245, 277, 2164, 2196
AloI	GAACNNNNNNTCC	2 cut(s)	1378, 1410
Alw21I	GWGCWC	4 cut(s)	191, 1063, 2736, 3158
Alw26I	GTCTC	8 cut(s)	566, 587, 771, 1901, 2190, 2201, 2286, 3376
Alw44I	GTGCAC	2 cut(s)	2732, 3154
AlwI	GGATC	5 cut(s)	1007, 1715, 1728, 2765, 3470
Ama87I	CYCGRG	1 cut(s)	3134
Aor13HI	TCCGGA	1 cut(s)	2284
ApaLI	GTGCAC	2 cut(s)	2732, 3154
ApeKI	GCWGC	6 cut(s)	28, 1070, 1640, 1655, 2514, 3050
Asp700I	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
AspLEI	GCGC	6 cut(s)	154, 320, 2947, 3275, 3337, 3541
AspS9I	GGNCC	4 cut(s)	101, 830, 1950, 3555
AsuHPI	GGTGA	7 cut(s)	370, 418, 1389, 2517, 2606, 3196, 3273
AvaI	CYCGRG	1 cut(s)	3134
AvaII	GGWCC	3 cut(s)	101, 830, 1950
BaeGI	GKGCMC	2 cut(s)	2736, 3158
BaeI	ACNNNNGTAYC	2 cut(s)	3233, 3266
BalI	TGGCCA	3 cut(s)	251, 1257, 1350
BamHI	GGATCC	1 cut(s)	1720
BanI	GGYRCC	3 cut(s)	1459, 1850, 3272
BanII	GRGCYC	1 cut(s)	1063
BarI	GAAGNNNNNNTAC	2 cut(s)	1815, 1847
BbrPI	CACGTG	1 cut(s)	3153
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	4 cut(s)	191, 1063, 2736, 3158
BbvCI	CCTCAGC	1 cut(s)	1946
BbvI	GCAGC	6 cut(s)	15, 1082, 1652, 1667, 2501, 3062
BceAI	ACGGC	7 cut(s)	1590, 2349, 2502, 3063, 3286, 3389, 3552
BcgI	CGANNNNNNTGC	2 cut(s)	440, 474
BciT130I	CCWGG	3 cut(s)	105, 2933, 3092
BciVI	GTATCC	2 cut(s)	2950, 3444
BclI	TGATCA	1 cut(s)	2421
BcoDI	GTCTC	8 cut(s)	566, 587, 771, 1901, 2190, 2201, 2286, 3376
BfaI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
BfoI	RGCGCY	1 cut(s)	3276
BfuAI	ACCTGC	1 cut(s)	1307
BfuI	GTATCC	2 cut(s)	2950, 3444
BglII	AGATCT	2 cut(s)	42, 2086
BlpI	GCTNAGC	1 cut(s)	2379
Bme1390I	CCNGG	3 cut(s)	105, 2933, 3092
Bme18I	GGWCC	3 cut(s)	101, 830, 1950
BmeT110I	CYCGRG	1 cut(s)	3134
BmgBI	CACGTC	1 cut(s)	2611
BmgT120I	GGNCC	4 cut(s)	101, 830, 1950, 3555
BmiI	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1722, 1852, 2139, 2602, 3274
BmrFI	CCNGG	3 cut(s)	105, 2933, 3092
BmrI	ACTGGG	2 cut(s)	1519, 2580
BmsI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
BmuI	ACTGGG	2 cut(s)	1519, 2580
BpiI	GAAGAC	1 cut(s)	693
BpmI	CTGGAG	1 cut(s)	2214
Bpu10I	CCTNAGC	1 cut(s)	1946
Bpu1102I	GCTNAGC	1 cut(s)	2379
BpuEI	CTTGAG	2 cut(s)	1421, 1997
Bsa29I	ATCGAT	1 cut(s)	2058
BsaAI	YACGTR	1 cut(s)	3153
BsaHI	GRCGYC	1 cut(s)	3273
BsaI	GGTCTC	2 cut(s)	566, 1901
BsaJI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BsaWI	WCCGGW	4 cut(s)	136, 2284, 2905, 3374
BsaXI	ACNNNNNCTCC	8 cut(s)	474, 504, 753, 783, 850, 880, 1378, 1408
Bsc4I	CCNNNNNNNGG	5 cut(s)	1469, 1730, 2713, 2983, 3374
Bse3DI	GCAATG	2 cut(s)	136, 2793
BseAI	TCCGGA	1 cut(s)	2284
BseBI	CCWGG	3 cut(s)	105, 2933, 3092
BseCI	ATCGAT	1 cut(s)	2058
BseDI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BseGI	GGATG	9 cut(s)	324, 539, 988, 1536, 2464, 2834, 2941, 3029, 3033
BseLI	CCNNNNNNNGG	5 cut(s)	1469, 1730, 2713, 2983, 3374
BseMI	GCAATG	2 cut(s)	136, 2793
BseMII	CTCAG	8 cut(s)	464, 787, 802, 1033, 1353, 1937, 2393, 2397
BseRI	GAGGAG	4 cut(s)	282, 518, 747, 761
BseSI	GKGCMC	2 cut(s)	2736, 3158
BseX3I	CGGCCG	2 cut(s)	3402, 3515
BseXI	GCAGC	6 cut(s)	15, 1082, 1652, 1667, 2501, 3062
BsgI	GTGCAG	2 cut(s)	2058, 3055
Bsh1236I	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
Bsh1285I	CGRYCG	3 cut(s)	2592, 3405, 3518
BshNI	GGYRCC	3 cut(s)	1459, 1850, 3272
BshVI	ATCGAT	1 cut(s)	2058
BsiEI	CGRYCG	3 cut(s)	2592, 3405, 3518
BsiHKAI	GWGCWC	4 cut(s)	191, 1063, 2736, 3158
BsiHKCI	CYCGRG	1 cut(s)	3134
BsiSI	CCGG	7 cut(s)	137, 1335, 1731, 2285, 2906, 3120, 3375
BsiWI	CGTACG	1 cut(s)	3267
BslFI	GGGAC	3 cut(s)	114, 816, 1442
BslI	CCNNNNNNNGG	5 cut(s)	1469, 1730, 2713, 2983, 3374
BsmAI	GTCTC	8 cut(s)	566, 587, 771, 1901, 2190, 2201, 2286, 3376
BsmFI	GGGAC	3 cut(s)	114, 816, 1442
BsmI	GAATGC	3 cut(s)	284, 700, 2670
Bso31I	GGTCTC	2 cut(s)	566, 1901
BsoBI	CYCGRG	1 cut(s)	3134
Bsp1286I	GDGCHC	4 cut(s)	191, 1063, 2736, 3158
Bsp13I	TCCGGA	1 cut(s)	2284
Bsp1407I	TGTACA	1 cut(s)	3147
Bsp1720I	GCTNAGC	1 cut(s)	2379
Bsp19I	CCATGG	2 cut(s)	354, 1911
BspCNI	CTCAG	8 cut(s)	465, 786, 801, 1032, 1354, 1938, 2392, 2398
BspDI	ATCGAT	1 cut(s)	2058
BspEI	TCCGGA	1 cut(s)	2284
BspFNI	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
BspHI	TCATGA	3 cut(s)	936, 1002, 1497
BspLI	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1722, 1852, 2139, 2602, 3274
BspMI	ACCTGC	1 cut(s)	1307
BspPI	GGATC	5 cut(s)	1007, 1715, 1728, 2765, 3470
BspT107I	GGYRCC	3 cut(s)	1459, 1850, 3272
BspTNI	GGTCTC	2 cut(s)	566, 1901
BsrDI	GCAATG	2 cut(s)	136, 2793
BsrGI	TGTACA	1 cut(s)	3147
BssECI	CCNNGG	5 cut(s)	354, 1351, 1911, 2331, 3133
BssNAI	GTATAC	1 cut(s)	198
BssNI	GRCGYC	1 cut(s)	3273
BssT1I	CCWWGG	3 cut(s)	354, 1351, 1911
Bst1107I	GTATAC	1 cut(s)	198
Bst2UI	CCWGG	3 cut(s)	105, 2933, 3092
Bst6I	CTCTTC	6 cut(s)	1899, 2819, 2902, 3005, 3227, 3260
BstACI	GRCGYC	1 cut(s)	3273
BstAUI	TGTACA	1 cut(s)	3147
BstBAI	YACGTR	1 cut(s)	3153
BstC8I	GCNNGC	7 cut(s)	1878, 2160, 2240, 2618, 2670, 2853, 3008
BstDSI	CCRYGG	3 cut(s)	354, 1911, 2331
BstF5I	GGATG	9 cut(s)	324, 539, 988, 1536, 2464, 2834, 2941, 3029, 3033
BstFNI	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
BstH2I	RGCGCY	1 cut(s)	3276
BstHHI	GCGC	6 cut(s)	154, 320, 2947, 3275, 3337, 3541
BstMAI	GTCTC	8 cut(s)	566, 587, 771, 1901, 2190, 2201, 2286, 3376
BstMCI	CGRYCG	3 cut(s)	2592, 3405, 3518
BstNI	CCWGG	3 cut(s)	105, 2933, 3092
BstNSI	RCATGY	3 cut(s)	1195, 2235, 2620
BstSCI	CCNGG	3 cut(s)	103, 2931, 3090
BstSLI	GKGCMC	2 cut(s)	2736, 3158
BstUI	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
BstV1I	GCAGC	6 cut(s)	15, 1082, 1652, 1667, 2501, 3062
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	4 cut(s)	42, 1720, 2086, 2757
BstXI	CCANNNNNNTGG	1 cut(s)	2611
BstYI	RGATCY	4 cut(s)	42, 1720, 2086, 2757
BstZ17I	GTATAC	1 cut(s)	198
BstZI	CGGCCG	2 cut(s)	3402, 3515
Bsu15I	ATCGAT	1 cut(s)	2058
BsuI	GTATCC	2 cut(s)	2950, 3444
BsuTUI	ATCGAT	1 cut(s)	2058
BtgI	CCRYGG	3 cut(s)	354, 1911, 2331
BtgZI	GCGATG	1 cut(s)	2311
BtrI	CACGTC	1 cut(s)	2611
BtsCI	GGATG	9 cut(s)	324, 539, 988, 1536, 2464, 2834, 2941, 3029, 3033
BtsI	GCAGTG	2 cut(s)	1706, 3297
BtsIMutI	CAGTG	6 cut(s)	270, 825, 1706, 1731, 2540, 3297
BveI	ACCTGC	1 cut(s)	1307
Cac8I	GCNNGC	7 cut(s)	1878, 2160, 2240, 2618, 2670, 2853, 3008
CciI	TCATGA	3 cut(s)	936, 1002, 1497
CciNI	GCGGCCGC	1 cut(s)	3515
CfoI	GCGC	6 cut(s)	154, 320, 2947, 3275, 3337, 3541
Cfr13I	GGNCC	4 cut(s)	101, 830, 1950, 3555
ClaI	ATCGAT	1 cut(s)	2058
CseI	GACGC	3 cut(s)	1699, 2379, 2708
Csp6I	GTAC	8 cut(s)	428, 706, 1195, 1230, 3148, 3268, 3430, 3457
CviQI	GTAC	8 cut(s)	428, 706, 1195, 1230, 3148, 3268, 3430, 3457
DinI	GGCGCC	1 cut(s)	3274
DraI	TTTAAA	2 cut(s)	603, 1483
DrdI	GACNNNNNNGTC	1 cut(s)	1493
DseDI	GACNNNNNNGTC	1 cut(s)	1493
EaeI	YGGCCR	6 cut(s)	249, 1255, 1332, 1348, 3402, 3515
EagI	CGGCCG	2 cut(s)	3402, 3515
Eam1104I	CTCTTC	6 cut(s)	1899, 2819, 2902, 3005, 3227, 3260
EarI	CTCTTC	6 cut(s)	1899, 2819, 2902, 3005, 3227, 3260
Ecl136II	GAGCTC	1 cut(s)	1061
EclXI	CGGCCG	2 cut(s)	3402, 3515
Eco130I	CCWWGG	3 cut(s)	354, 1351, 1911
Eco147I	AGGCCT	1 cut(s)	3095
Eco24I	GRGCYC	1 cut(s)	1063
Eco31I	GGTCTC	2 cut(s)	566, 1901
Eco32I	GATATC	2 cut(s)	2635, 3130
Eco47I	GGWCC	3 cut(s)	101, 830, 1950
Eco52I	CGGCCG	2 cut(s)	3402, 3515
Eco53kI	GAGCTC	1 cut(s)	1061
Eco57I	CTGAAG	2 cut(s)	1145, 2986
Eco72I	CACGTG	1 cut(s)	3153
Eco88I	CYCGRG	1 cut(s)	3134
EcoICRI	GAGCTC	1 cut(s)	1061
EcoO109I	RGGNCCY	1 cut(s)	1950
EcoRI	GAATTC	3 cut(s)	509, 1676, 2026
EcoRII	CCWGG	3 cut(s)	103, 2931, 3090
EcoRV	GATATC	2 cut(s)	2635, 3130
EcoT14I	CCWWGG	3 cut(s)	354, 1351, 1911
EcoT38I	GRGCYC	1 cut(s)	1063
EgeI	GGCGCC	1 cut(s)	3274
EheI	GGCGCC	1 cut(s)	3274
ErhI	CCWWGG	3 cut(s)	354, 1351, 1911
FalI	AAGNNNNNCTT	2 cut(s)	2367, 2399
FaqI	GGGAC	3 cut(s)	114, 816, 1442
FauI	CCCGC	1 cut(s)	3340
FauNDI	CATATG	2 cut(s)	156, 1615
FbaI	TGATCA	1 cut(s)	2421
FblI	GTMKAC	2 cut(s)	197, 2722
FokI	GGATG	9 cut(s)	311, 526, 995, 1523, 2471, 2821, 2948, 3036, 3040
FriOI	GRGCYC	1 cut(s)	1063
FspBI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
GlaI	GCGC	6 cut(s)	153, 319, 2946, 3274, 3336, 3540
GsuI	CTGGAG	1 cut(s)	2214
HaeII	RGCGCY	1 cut(s)	3276
HapII	CCGG	7 cut(s)	137, 1335, 1731, 2285, 2906, 3120, 3375
HgaI	GACGC	3 cut(s)	1699, 2379, 2708
HhaI	GCGC	6 cut(s)	154, 320, 2947, 3275, 3337, 3541
Hin1I	GRCGYC	1 cut(s)	3273
Hin6I	GCGC	6 cut(s)	152, 318, 2945, 3273, 3335, 3539
HinP1I	GCGC	6 cut(s)	152, 318, 2945, 3273, 3335, 3539
HincII	GTYRAC	1 cut(s)	1264
HindII	GTYRAC	1 cut(s)	1264
HindIII	AAGCTT	1 cut(s)	2156
HinfI	GANTC	9 cut(s)	140, 469, 580, 647, 779, 1270, 1625, 1957, 2279
HpaII	CCGG	7 cut(s)	137, 1335, 1731, 2285, 2906, 3120, 3375
HphI	GGTGA	7 cut(s)	370, 418, 1389, 2517, 2606, 3196, 3273
Hpy99I	CGWCG	1 cut(s)	2395
HpyCH4IV	ACGT	4 cut(s)	1751, 2610, 2886, 3152
HpySE526I	ACGT	4 cut(s)	1751, 2610, 2886, 3152
Hsp92I	GRCGYC	1 cut(s)	3273
HspAI	GCGC	6 cut(s)	152, 318, 2945, 3273, 3335, 3539
KasI	GGCGCC	1 cut(s)	3272
Kpn2I	TCCGGA	1 cut(s)	2284
Ksp22I	TGATCA	1 cut(s)	2421
LmnI	GCTCC	8 cut(s)	186, 304, 812, 1066, 1915, 2600, 2625, 2716
Lsp1109I	GCAGC	6 cut(s)	15, 1082, 1652, 1667, 2501, 3062
LweI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
MaeI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
MaeII	ACGT	4 cut(s)	1751, 2610, 2886, 3152
MaeIII	GTNAC	9 cut(s)	268, 625, 2065, 2545, 2747, 2874, 3197, 3279, 3488
MfeI	CAATTG	2 cut(s)	2490, 3237
MflI	RGATCY	4 cut(s)	42, 1720, 2086, 2757
MhlI	GDGCHC	4 cut(s)	191, 1063, 2736, 3158
MlsI	TGGCCA	3 cut(s)	251, 1257, 1350
MluNI	TGGCCA	3 cut(s)	251, 1257, 1350
Mly113I	GGCGCC	1 cut(s)	3273
MlyI	GAGTC	4 cut(s)	134, 641, 773, 2288
MmeI	TCCRAC	5 cut(s)	542, 697, 1698, 2280, 2623
Mox20I	TGGCCA	3 cut(s)	251, 1257, 1350
MroI	TCCGGA	1 cut(s)	2284
MroXI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
MscI	TGGCCA	3 cut(s)	251, 1257, 1350
MslI	CAYNNNNRTG	2 cut(s)	1419, 1923
Msp20I	TGGCCA	3 cut(s)	251, 1257, 1350
MspA1I	CMGCKG	2 cut(s)	311, 3006
MspI	CCGG	7 cut(s)	137, 1335, 1731, 2285, 2906, 3120, 3375
MspR9I	CCNGG	3 cut(s)	105, 2933, 3092
MunI	CAATTG	2 cut(s)	2490, 3237
Mva1269I	GAATGC	3 cut(s)	284, 700, 2670
MvaI	CCWGG	3 cut(s)	105, 2933, 3092
MvnI	CGCG	6 cut(s)	320, 2046, 2945, 2947, 3335, 3541
NarI	GGCGCC	1 cut(s)	3273
NcoI	CCATGG	2 cut(s)	354, 1911
NdeI	CATATG	2 cut(s)	156, 1615
NlaIV	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1722, 1852, 2139, 2602, 3274
NmeAIII	GCCGAG	1 cut(s)	3380
NmuCI	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
NotI	GCGGCCGC	1 cut(s)	3515
NspI	RCATGY	3 cut(s)	1195, 2235, 2620
PaeI	GCATGC	1 cut(s)	2620
PagI	TCATGA	3 cut(s)	936, 1002, 1497
PceI	AGGCCT	1 cut(s)	3095
PciI	ACATGT	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	1 cut(s)	3149
PctI	GAATGC	3 cut(s)	284, 700, 2670
PdmI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
PfeI	GAWTC	5 cut(s)	469, 580, 1270, 1625, 1957
Pfl23II	CGTACG	1 cut(s)	3267
PflMI	CCANNNNNTGG	1 cut(s)	2713
Ple19I	CGATCG	1 cut(s)	2592
PleI	GAGTC	4 cut(s)	134, 641, 773, 2287
PluTI	GGCGCC	1 cut(s)	3276
PmaCI	CACGTG	1 cut(s)	3153
PmlI	CACGTG	1 cut(s)	3153
PpsI	GAGTC	4 cut(s)	134, 641, 773, 2287
Ppu21I	YACGTR	1 cut(s)	3153
PpuMI	RGGWCCY	1 cut(s)	1950
PscI	ACATGT	1 cut(s)	1191
Psp124BI	GAGCTC	1 cut(s)	1063
Psp5II	RGGWCCY	1 cut(s)	1950
Psp6I	CCWGG	3 cut(s)	103, 2931, 3090
PspCI	CACGTG	1 cut(s)	3153
PspGI	CCWGG	3 cut(s)	103, 2931, 3090
PspLI	CGTACG	1 cut(s)	3267
PspN4I	GGNNCC	9 cut(s)	102, 300, 832, 1461, 1722, 1852, 2139, 2602, 3274
PspPI	GGNCC	4 cut(s)	101, 830, 1950, 3555
PspPPI	RGGWCCY	1 cut(s)	1950
PsuI	RGATCY	4 cut(s)	42, 1720, 2086, 2757
PvuI	CGATCG	1 cut(s)	2592
PvuII	CAGCTG	1 cut(s)	3006
RsaI	GTAC	8 cut(s)	429, 707, 1196, 1231, 3149, 3269, 3431, 3458
RsaNI	GTAC	8 cut(s)	428, 706, 1195, 1230, 3148, 3268, 3430, 3457
RseI	CAYNNNNRTG	2 cut(s)	1419, 1923
SacI	GAGCTC	1 cut(s)	1063
Sau96I	GGNCC	4 cut(s)	101, 830, 1950, 3555
SchI	GAGTC	4 cut(s)	134, 641, 773, 2288
ScrFI	CCNGG	3 cut(s)	105, 2933, 3092
SduI	GDGCHC	4 cut(s)	191, 1063, 2736, 3158
SfaNI	GCATC	6 cut(s)	544, 1039, 1927, 1927, 2345, 2510
SfoI	GGCGCC	1 cut(s)	3274
SinI	GGWCC	3 cut(s)	101, 830, 1950
SmiMI	CAYNNNNRTG	2 cut(s)	1419, 1923
SmlI	CTYRAG	2 cut(s)	1436, 1976
SmoI	CTYRAG	2 cut(s)	1436, 1976
SphI	GCATGC	1 cut(s)	2620
SseBI	AGGCCT	1 cut(s)	3095
SspDI	GGCGCC	1 cut(s)	3272
SspI	AATATT	3 cut(s)	1609, 2995, 3018
SspMI	CTAG	4 cut(s)	1035, 2550, 2585, 3083
SstI	GAGCTC	1 cut(s)	1063
StuI	AGGCCT	1 cut(s)	3095
StyD4I	CCNGG	3 cut(s)	103, 2931, 3090
StyI	CCWWGG	3 cut(s)	354, 1351, 1911
TaiI	ACGT	4 cut(s)	1754, 2613, 2889, 3155
TatI	WGTACW	4 cut(s)	427, 705, 1194, 3147
TauI	GCSGC	5 cut(s)	385, 3502, 3517, 3520, 3551
TfiI	GAWTC	5 cut(s)	469, 580, 1270, 1625, 1957
TscAI	CASTG	6 cut(s)	277, 832, 1713, 1731, 2547, 3304
TseFI	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
TseI	GCWGC	6 cut(s)	28, 1070, 1640, 1655, 2514, 3050
Tsp45I	GTSAC	5 cut(s)	268, 2545, 2874, 3279, 3488
TspGWI	ACGGA	2 cut(s)	1053, 3463
TspRI	CASTG	6 cut(s)	277, 832, 1713, 1731, 2547, 3304
Van91I	CCANNNNNTGG	1 cut(s)	2713
VneI	GTGCAC	2 cut(s)	2732, 3154
VpaK11BI	GGWCC	3 cut(s)	101, 830, 1950
XbaI	TCTAGA	1 cut(s)	1034
XceI	RCATGY	3 cut(s)	1195, 2235, 2620
XmiI	GTMKAC	2 cut(s)	197, 2722
XmnI	GAANNNNTTC	4 cut(s)	513, 1393, 1547, 1888
XspI	CTAG	4 cut(s)	1035, 2550, 2585, 3083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.