Basic Information

Type: Sequence Only

Biological Identity

Species

rosa_samantha

Chromosome

Unknown

Orthogroup ID

OG0003148

Physical Location & Seq

Genomic Coordinates Reverse (-)

0 .. 0

Gene Structure 1 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG371300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3555 bp

ATGAGGAGTGGTGGGAGAAAGAGAAGGCTGCATTTCAGCAAGATCTATTCTTTCACTTGCGGTAGAGCTTCTTTGAAGGAGGAGCATTCACAGATTGGAGGGCCAGGATTTTCCAGAGTGGTTTTCTGCAACGAACCGGATTCGTTTGAGGCCGGGATTCGAAACTATGCAGTTAATTATGTCAGCACTACTAAGTACACACTCGCTACATTCTTTCCCAAGTCTTTGTTTGAGCAGTTCAGGAGGGTAGCTAACTTCTTCTTCTTGGTCACTGGCGTTTTGGCCTTCACTCCTCTGGCTCCGTATTCAGCTGTCAGTGCCATAATTCCTCTGATCATCATCATTAGCGCCACGATGGTGAAGGAGGCGATTGAAGATTGGCGTCGAAAACAGCAGGATGTTGAAGTGAACAATAGAAAGGTTAAAGTGCATAAGGGTAGTGGCAATTTTGATTATACTGAATGGAAGAATCTGAGAGTGGGAGATATAGTGAGAGTGGAAAAGGATGAATTCTTTCCAACAGATCTCCTGTTACTTTCGTCCAGTTACGAAGATGCAATCTGCTATGTTGAGACAATGAACCTTGATGGGGAGACAAACTTAAAGTTGAAACAAGCATTGGAGGTAACTTCACCGTTACAGGAGGACACCAGCATCAGCAATTTTCATGCCATGGTGAAATGTGAGGACCCAAATGCAAATTTGTACTCATTTGTCGGAACTATGGAGTTTGAAAAGCAGCAGTATTCCCTCTCTCCTCAACAGCTTCTTCTCAGAGACTCTAAACTCCGAAATACAGACTACATATACGGAGTGGTTATCTTCACCGGTGTTGATACAAAGGTTATTCAAAACTCTACTCCCCCTCCTTCAAAAAGAAGCAGAGTTGAGAAGAAGATGGATCAAATTATCTACGTCTTGTTTGGTGTTTTATTTACATTGGCATCAGTTGGATCCATTTTCTTTGGCATTACAACCAAAGATGACATAAACAATGGGATCATGAAAAGGTGGTATCTGAAGCCAGATAGTTCTGAAATTTTCTATGATCCCAAAAAGGCTCCTGTTGCAGCAATTTATCACTTTTTGACTGCTCTGATGTTATATAACTACTTCATCCCTATCTCCTTGTATGTGTCAATTGAAATTGTTAAAGTTCTTCAGAGCGTCTTCATCAATCAAGATATTCACATGTACTATGAAGAAGCTGATAAACCAGCACATGCCCGTACCTCAAACTTGAATGAAGAACTTGGCCAAGTTGACACAATTCTTTCAGACAAGACTGGAACTTTGACATGCAATTCAATGGAGTTCATCAAATGTTCTGTGGCTGGAACAGCTTACGGCCGTGGTTTTACTGAGGTTGAGAGGTCTATGGGCAGGCGAAATGGTTCACCAATTCATGAAGCTGTAAGTGGAGGGAATGAAACAACGACACCCGTTAAAGGCTTTAATTTTAAAGATGAAAGGATCATGAATGGAAATTGGGTTGATGAGCCTCATGGAGATCTCATCCAGAAATTTTTCCGCTTATTAGCAGTCTGCCATACAGCCATTCCAGAAGTTGATGATGTAACTGGGAAGGTTATGTATGAGGCTGAGTCTCCTGATGAAGCAGCATTTGTGATTGCAGCAAGAGAACTTGGTTTTGAATTCTATAAAAGGACACAAACAAGCATATCAGTGAGAGAATTGGACAGAGCCTCTGGAAAGAAAGTTGATAGGTTGTACACTCTCCTGAATGTGTTAGAATTCAACAGCTCAAGGAAACGGATGTCTGTGATTGTAAGAAATGAGGAAGGAAAAGTACTATTGCTCTGTAAAGGTGCCGACAATGTCATGTTTGAAAGACTTGCCAAAAATGGTAGGGAGTTTGAAGAAGAGACCAAGGAGCATTTAAATGGGTATGCTGATGCAGGGCTAAGGACTTTGATACTTGCCTATCGTGAACTTCAGGAAGATGAATACAGAGAGTTTAATGCAAAATTTATCAAGGCCAAGAACTCAATTGGTGCAGACCGTGAAACATTGATTGATGAAGTAACAGATAATGTTGAAAAAGATCTAATTCTTCTTGGTGCGACAGCTGTTGAGGACAAACTTCAAAATGGGGTTCCCGATTGCATCGACAAGCTTGCCCAAGCAGGAATTAAGATTTGGGTTTTGACTGGAGACAAGATGGAAACTGCCATCAACATTGGGTTTGCCTGTAGCTTACTTAGACAAGGAATGAAACAGGTTATGATAAATTTGGAGTCTCCAGAAATTAAGGTGCTGGAAAAGGAAGGAGATAAGGATGCCATTACCAAGGCATCAAGAGCACGTGTCTTCCATCAGATAACCAAGGGAAAGGCCCAGCTTACAGCATCAAGTGGGGGATCCGAGGCATATGCATTGATCATTGATGGAAAATCTCTTGCTTATGCTCTTGAGGATGATATAAAGTCATTGTTTCTAGAGCTTGCAATGGGCTGTGCATCTGTTATTTGTTGCCGTTCGTCCCCAAAACAGAAAGCACTGGTCACTAGACTGGTCAAATCTGGAACTGGGAAAACAACTCTTGCGATTGGTGATGGCGCCAATGATGTGGGAATGCTCCAAGAAGCAGATATTGGGATTGGAATTAGTGGTGTTGAAGGAATGCAGGCGGTCATGTCAAGTGATATTGCGATTGCACAATTTCAATATTTGGAGCGTCTGCTGCTTGTGCATGGACATTGGTGTTACAGGAGGATCTCATCAATGATTTGCTACTTCTTCTACAAGAACATCACATTTGGTTTAACTATCTTCTTATATGAGGCATCCACATCCTTCTCTGGACAACCTGCATACAATGACTGGTTTTTATCACTGTATAACGTATTCTTCTCATCGCTTCCAGTGGTTGCCATGGGAGTTTTCGACCAGGATGTTTCTGCACGATTTTGTCTCAAGTTTCCTCTAATATACCAAGAAGGGGTGCAAAATGTTCTCTTCAGCTGGCGTAGAATAATCGGTTGGATGTTGAATGGATTAACAAGTGCAGTGATTATTTTCTTTTTCTGCATGAAAGCGCTACAACCATATGCCTTCAATCAGGATGGGAAAACTGCTGGGAAGGATATTCTAGGAGCAACAATGTACACATGCATCGTTTGGGTTGTGAACTTGCAGATGGCACTTGCCATAAGTTACTTCACCTTGATACAACACCTTTTCATCTGGGGTTCCATTGTTCTCTGGTATCTTTTTATGTTAGCATATGGAGCCATGTCACCCACGCTCTCTACCAATGCATACAAAGTCTTCGTCGAAACCCTTGCCCCCGCCCCTTCTTTCTGGCTTATTACATTGCTTGTGCCAATCTCAGCTCTACTCCCATATTTCACATACTCATCACTTCGGATGCGGTTCTTTCCTTTGTATCATGAAATAATACAATGGATTAGGTACGAGGGGCGGTCAAATGATCCAGAGTTTTGTGATATGGTGCGGCAGAGATCTTTGCGGCCAACAACCGTGGGTTTCACAGCACGGTTAGCTGCTAAGACCAACATTATAAGAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1184

Amino Acids

133.62

Weight (kDa)

6.97

Isoelectric Point (pI)

36.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 108	9.7e-21	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	508 - 703	7.3e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	885 - 1135	3.2e-87	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13210 AT1G26130 AT1G26130 AT1G26130 AT1G26130 AT1G68710 AT1G68710 AT1G68710 AT3G25610
fragaria_vesca	FvH4_4g28450 FvH4_5g12920 FvH4_5g12920
malus_domestica	MD06G1209100.v1.1 MD13G1050400.v1.1 MD14G1219500.v1.1 MD16G1051100.v1.1
prunus_persica	Prupe.1G301500_v2.0.a1 Prupe.5G214200_v2.0.a1
pyrus_communis	pycom06g18670 pycom14g18190 pycom16g04490
rosa_chinensis	RchiOBHm_Chr4g0436411 RchiOBHm_Chr7g0184301
rosa_laevigata	RLG00000004988 RLG00000006521
rosa_multiflora	Rmu_sc0000579.1_g000001 Rmu_sc0013812.1_g000007
rosa_roxburghii	Rroxscaffold_3G00269750 Rroxscaffold_5G00377570
rosa_rugosa	Rorug04G0293600
rosa_samantha	Rh4AG348000 Rh4CG371300 Rh7AG081300 Rh7CG066300 Rh7DG065900
rosa_wichuraiana	Rw4G030420 Rw7G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3545
Acc36I	ACCTGC	1 cut(s)	2839
AccB1I	GGYRCC	2 cut(s)	1829, 2579
AciI	CCGC	8 cut(s)	60, 1531, 2651, 3312, 3394, 3445, 3478, 3493
AcoI	YGGCCR	3 cut(s)	1255, 1348, 3494
AcsI	RAATTY	8 cut(s)	509, 700, 1038, 1523, 1655, 1754, 1988, 2251
AcuI	CTGAAG	4 cut(s)	1040, 1145, 1940, 2965
AcvI	CACGTG	1 cut(s)	2327
AcyI	GRCGYC	2 cut(s)	382, 2580
AfaI	GTAC	8 cut(s)	197, 707, 1196, 1231, 1733, 1812, 3128, 3437
AfeI	AGCGCT	1 cut(s)	3060
AfiI	CCNNNNNNNGG	3 cut(s)	589, 1448, 2962
AflIII	ACRYGT	2 cut(s)	1191, 2326
AgeI	ACCGGT	1 cut(s)	827
AjnI	CCWGG	2 cut(s)	103, 2910
AjuI	GAANNNNNNNTTGG	8 cut(s)	602, 634, 2143, 2175, 2598, 2630, 2986, 3018
AleI	CACNNNNGTG	1 cut(s)	356
Alw21I	GWGCWC	1 cut(s)	2326
Alw26I	GTCTC	8 cut(s)	566, 587, 771, 1611, 1880, 2169, 2265, 2939
Aor51HI	AGCGCT	1 cut(s)	3060
AoxI	GGCC	8 cut(s)	101, 150, 282, 1255, 1348, 1998, 2355, 3494
ApeKI	GCWGC	7 cut(s)	28, 739, 1070, 1619, 1634, 2704, 3527
ApoI	RAATTY	8 cut(s)	509, 700, 1038, 1523, 1655, 1754, 1988, 2251
ArsI	GACNNNNNNTTYG	4 cut(s)	1276, 1308, 3279, 3311
AsiGI	ACCGGT	1 cut(s)	827
Asp700I	GAANNNNTTC	3 cut(s)	513, 1393, 1526
AspLEI	GCGC	3 cut(s)	350, 2582, 3061
AspS9I	GGNCC	3 cut(s)	101, 688, 2356
AsuC2I	CCSGG	1 cut(s)	154
AsuHPI	GGTGA	8 cut(s)	370, 624, 688, 817, 1389, 2585, 3175, 3252
AsuII	TTCGAA	1 cut(s)	160
AvaII	GGWCC	1 cut(s)	688
BaeI	ACNNNNGTAYC	2 cut(s)	828, 861
BalI	TGGCCA	1 cut(s)	1257
BamHI	GGATCC	2 cut(s)	953, 2381
BanI	GGYRCC	2 cut(s)	1829, 2579
BarI	GAAGNNNNNNTAC	2 cut(s)	1794, 1826
BbrPI	CACGTG	1 cut(s)	2327
BbsI	GAAGAC	3 cut(s)	1162, 2323, 3283
Bbv12I	GWGCWC	1 cut(s)	2326
BbvI	GCAGC	7 cut(s)	15, 751, 1082, 1631, 1646, 2691, 3514
BceAI	ACGGC	3 cut(s)	1335, 1363, 2481
BcgI	CGANNNNNNTGC	6 cut(s)	2120, 2154, 2375, 2409, 3287, 3321
BciT130I	CCWGG	2 cut(s)	105, 2912
BclI	TGATCA	2 cut(s)	333, 2400
BcnI	CCSGG	1 cut(s)	154
BcoDI	GTCTC	8 cut(s)	566, 587, 771, 1611, 1880, 2169, 2265, 2939
BfaI	CTAG	3 cut(s)	2459, 2529, 3113
BfmI	CTRYAG	1 cut(s)	2212
BfoI	RGCGCY	3 cut(s)	351, 2583, 3062
BfuAI	ACCTGC	1 cut(s)	2839
BglII	AGATCT	5 cut(s)	42, 523, 1510, 2065, 3485
BisI	GCNGC	9 cut(s)	29, 740, 1071, 1620, 1635, 2705, 3479, 3494, 3528
BlsI	GCNGC	9 cut(s)	30, 741, 1072, 1621, 1636, 2706, 3480, 3495, 3529
BmcAI	AGTACT	1 cut(s)	1812
Bme1390I	CCNGG	3 cut(s)	105, 154, 2912
Bme18I	GGWCC	1 cut(s)	688
BmgT120I	GGNCC	3 cut(s)	101, 688, 2356
BmrFI	CCNGG	3 cut(s)	105, 154, 2912
BmrI	ACTGGG	2 cut(s)	1590, 2559
BmuI	ACTGGG	2 cut(s)	1590, 2559
BpiI	GAAGAC	3 cut(s)	1162, 2323, 3283
BpmI	CTGGAG	2 cut(s)	2193, 2247
Bpu10I	CCTNAGC	1 cut(s)	1925
Bpu14I	TTCGAA	1 cut(s)	160
BpuEI	CTTGAG	3 cut(s)	1750, 2453, 2921
BpuMI	CCSGG	1 cut(s)	154
BsaAI	YACGTR	1 cut(s)	2327
BsaBI	GATNNNNATC	1 cut(s)	338
BsaHI	GRCGYC	2 cut(s)	382, 2580
BsaI	GGTCTC	1 cut(s)	1880
BsaJI	CCNNGG	8 cut(s)	672, 1351, 1890, 2310, 2346, 2385, 2895, 3504
BsaWI	WCCGGW	2 cut(s)	136, 827
BsaXI	ACNNNNNCTCC	4 cut(s)	474, 504, 850, 880
Bsc4I	CCNNNNNNNGG	3 cut(s)	589, 1448, 2962
Bse118I	RCCGGY	1 cut(s)	827
Bse3DI	GCAATG	2 cut(s)	2475, 3335
Bse8I	GATNNNNATC	1 cut(s)	338
BseBI	CCWGG	2 cut(s)	105, 2912
BseDI	CCNNGG	8 cut(s)	672, 1351, 1890, 2310, 2346, 2385, 2895, 3504
BseJI	GATNNNNATC	1 cut(s)	338
BseLI	CCNNNNNNNGG	3 cut(s)	589, 1448, 2962
BseMI	GCAATG	2 cut(s)	2475, 3335
BseMII	CTCAG	5 cut(s)	464, 787, 1353, 1593, 3366
BseRI	GAGGAG	4 cut(s)	19, 95, 282, 747
BseX3I	CGGCCG	1 cut(s)	1348
BseXI	GCAGC	7 cut(s)	15, 751, 1082, 1631, 1646, 2691, 3514
BseYI	CCCAGC	2 cut(s)	2358, 3098
BsgI	GTGCAG	3 cut(s)	2037, 2907, 3048
Bsh1285I	CGRYCG	1 cut(s)	1351
BshFI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 2000, 2357, 3496
BshNI	GGYRCC	2 cut(s)	1829, 2579
BshTI	ACCGGT	1 cut(s)	827
BsiEI	CGRYCG	1 cut(s)	1351
BsiHKAI	GWGCWC	1 cut(s)	2326
BsiSI	CCGG	3 cut(s)	137, 153, 828
BslFI	GGGAC	1 cut(s)	2488
BslI	CCNNNNNNNGG	3 cut(s)	589, 1448, 2962
BsmAI	GTCTC	8 cut(s)	566, 587, 771, 1611, 1880, 2169, 2265, 2939
BsmFI	GGGAC	1 cut(s)	2488
BsmI	GAATGC	3 cut(s)	85, 2601, 2649
BsnI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 2000, 2357, 3496
Bso31I	GGTCTC	1 cut(s)	1880
Bsp119I	TTCGAA	1 cut(s)	160
Bsp1286I	GDGCHC	1 cut(s)	2326
Bsp1407I	TGTACA	2 cut(s)	1731, 3126
Bsp19I	CCATGG	2 cut(s)	672, 2895
BspACI	CCGC	8 cut(s)	60, 1531, 2651, 3312, 3394, 3445, 3478, 3493
BspANI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 2000, 2357, 3496
BspCNI	CTCAG	5 cut(s)	465, 786, 1354, 1594, 3365
BspHI	TCATGA	4 cut(s)	1002, 1405, 1476, 3412
BspMI	ACCTGC	1 cut(s)	2839
BspT104I	TTCGAA	1 cut(s)	160
BspT107I	GGYRCC	2 cut(s)	1829, 2579
BspTNI	GGTCTC	1 cut(s)	1880
BsrDI	GCAATG	2 cut(s)	2475, 3335
BsrFI	RCCGGY	1 cut(s)	827
BsrGI	TGTACA	2 cut(s)	1731, 3126
BssAI	RCCGGY	1 cut(s)	827
BssECI	CCNNGG	8 cut(s)	672, 1351, 1890, 2310, 2346, 2385, 2895, 3504
BssNI	GRCGYC	2 cut(s)	382, 2580
BssT1I	CCWWGG	5 cut(s)	672, 1890, 2310, 2346, 2895
Bst2UI	CCWGG	2 cut(s)	105, 2912
Bst4CI	ACNGT	5 cut(s)	636, 2024, 2859, 3505, 3522
Bst6I	CTCTTC	2 cut(s)	1878, 2984
BstACI	GRCGYC	2 cut(s)	382, 2580
BstAUI	TGTACA	2 cut(s)	1731, 3126
BstBAI	YACGTR	1 cut(s)	2327
BstBI	TTCGAA	1 cut(s)	160
BstC8I	GCNNGC	5 cut(s)	1385, 2139, 2466, 2649, 2987
BstDEI	CTNAG	9 cut(s)	192, 473, 773, 1362, 1602, 1925, 2222, 3352, 3531
BstDSI	CCRYGG	4 cut(s)	672, 1351, 2895, 3504
BstH2I	RGCGCY	3 cut(s)	351, 2583, 3062
BstHHI	GCGC	3 cut(s)	350, 2582, 3061
BstMAI	GTCTC	8 cut(s)	566, 587, 771, 1611, 1880, 2169, 2265, 2939
BstMCI	CGRYCG	1 cut(s)	1351
BstMWI	GCNNNNNNNGC	8 cut(s)	317, 1067, 1340, 2321, 2704, 2710, 3251, 3524
BstNI	CCWGG	2 cut(s)	105, 2912
BstNSI	RCATGY	4 cut(s)	1195, 1226, 1302, 3135
BstSCI	CCNGG	3 cut(s)	103, 152, 2910
BstSFI	CTRYAG	1 cut(s)	2212
BstV1I	GCAGC	7 cut(s)	15, 751, 1082, 1631, 1646, 2691, 3514
BstV2I	GAAGAC	3 cut(s)	1162, 2323, 3283
BstX2I	RGATCY	8 cut(s)	42, 523, 953, 1510, 2065, 2381, 2736, 3485
BstXI	CCANNNNNNTGG	1 cut(s)	2590
BstYI	RGATCY	8 cut(s)	42, 523, 953, 1510, 2065, 2381, 2736, 3485
BstZI	CGGCCG	1 cut(s)	1348
BsuRI	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 2000, 2357, 3496
BtgI	CCRYGG	4 cut(s)	672, 1351, 2895, 3504
BtgZI	GCGATG	1 cut(s)	2862
BtsI	GCAGTG	1 cut(s)	3036
BtsIMutI	CAGTG	7 cut(s)	270, 322, 1692, 2519, 2855, 2892, 3036
BveI	ACCTGC	1 cut(s)	2839
Cac8I	GCNNGC	5 cut(s)	1385, 2139, 2466, 2649, 2987
CciI	TCATGA	4 cut(s)	1002, 1405, 1476, 3412
CfoI	GCGC	3 cut(s)	350, 2582, 3061
Cfr10I	RCCGGY	1 cut(s)	827
Cfr13I	GGNCC	3 cut(s)	101, 688, 2356
CseI	GACGC	3 cut(s)	371, 1156, 2687
Csp6I	GTAC	8 cut(s)	196, 706, 1195, 1230, 1732, 1811, 3127, 3436
CspAI	ACCGGT	1 cut(s)	827
CspCI	CAANNNNNGTGG	2 cut(s)	3486, 3521
CviQI	GTAC	8 cut(s)	196, 706, 1195, 1230, 1732, 1811, 3127, 3436
DdeI	CTNAG	9 cut(s)	192, 473, 773, 1362, 1602, 1925, 2222, 3352, 3531
DinI	GGCGCC	1 cut(s)	2581
DraI	TTTAAA	2 cut(s)	1462, 1902
EaeI	YGGCCR	3 cut(s)	1255, 1348, 3494
EagI	CGGCCG	1 cut(s)	1348
Eam1104I	CTCTTC	2 cut(s)	1878, 2984
EarI	CTCTTC	2 cut(s)	1878, 2984
EclXI	CGGCCG	1 cut(s)	1348
Eco130I	CCWWGG	5 cut(s)	672, 1890, 2310, 2346, 2895
Eco31I	GGTCTC	1 cut(s)	1880
Eco47I	GGWCC	1 cut(s)	688
Eco47III	AGCGCT	1 cut(s)	3060
Eco52I	CGGCCG	1 cut(s)	1348
Eco57I	CTGAAG	4 cut(s)	1040, 1145, 1940, 2965
Eco72I	CACGTG	1 cut(s)	2327
EcoO109I	RGGNCCY	1 cut(s)	688
EcoRI	GAATTC	3 cut(s)	509, 1655, 1754
EcoRII	CCWGG	2 cut(s)	103, 2910
EcoT14I	CCWWGG	5 cut(s)	672, 1890, 2310, 2346, 2895
EcoT22I	ATGCAT	3 cut(s)	2398, 3137, 3283
EgeI	GGCGCC	1 cut(s)	2581
EheI	GGCGCC	1 cut(s)	2581
ErhI	CCWWGG	5 cut(s)	672, 1890, 2310, 2346, 2895
FaqI	GGGAC	1 cut(s)	2488
FauI	CCCGC	1 cut(s)	3319
FauNDI	CATATG	3 cut(s)	2392, 3070, 3247
FbaI	TGATCA	2 cut(s)	333, 2400
Fnu4HI	GCNGC	9 cut(s)	29, 740, 1071, 1620, 1635, 2705, 3479, 3494, 3528
Fsp4HI	GCNGC	9 cut(s)	29, 740, 1071, 1620, 1635, 2705, 3479, 3494, 3528
FspBI	CTAG	3 cut(s)	2459, 2529, 3113
GlaI	GCGC	3 cut(s)	349, 2581, 3060
GluI	GCNGC	9 cut(s)	29, 740, 1071, 1620, 1635, 2705, 3479, 3494, 3528
GsaI	CCCAGC	2 cut(s)	2362, 3102
GsuI	CTGGAG	2 cut(s)	2193, 2247
HaeII	RGCGCY	3 cut(s)	351, 2583, 3062
HaeIII	GGCC	8 cut(s)	103, 152, 284, 1257, 1350, 2000, 2357, 3496
HapII	CCGG	3 cut(s)	137, 153, 828
HgaI	GACGC	3 cut(s)	371, 1156, 2687
HhaI	GCGC	3 cut(s)	350, 2582, 3061
Hin1I	GRCGYC	2 cut(s)	382, 2580
Hin6I	GCGC	3 cut(s)	348, 2580, 3059
HinP1I	GCGC	3 cut(s)	348, 2580, 3059
HincII	GTYRAC	1 cut(s)	1264
HindII	GTYRAC	1 cut(s)	1264
HindIII	AAGCTT	1 cut(s)	2135
HinfI	GANTC	6 cut(s)	140, 157, 469, 779, 1604, 2258
HpaII	CCGG	3 cut(s)	137, 153, 828
HphI	GGTGA	8 cut(s)	370, 624, 688, 817, 1389, 2585, 3175, 3252
Hpy166II	GTNNAC	8 cut(s)	198, 409, 1264, 1397, 1734, 1952, 3129, 3151
Hpy8I	GTNNAC	8 cut(s)	198, 409, 1264, 1397, 1734, 1952, 3129, 3151
Hpy99I	CGWCG	2 cut(s)	387, 3299
HpyCH4III	ACNGT	5 cut(s)	636, 2024, 2859, 3505, 3522
HpyCH4IV	ACGT	3 cut(s)	915, 2326, 2865
HpyF10VI	GCNNNNNNNGC	8 cut(s)	317, 1067, 1340, 2321, 2704, 2710, 3251, 3524
HpyF3I	CTNAG	9 cut(s)	192, 473, 773, 1362, 1602, 1925, 2222, 3352, 3531
HpySE526I	ACGT	3 cut(s)	915, 2326, 2865
Hsp92I	GRCGYC	2 cut(s)	382, 2580
HspAI	GCGC	3 cut(s)	348, 2580, 3059
KasI	GGCGCC	1 cut(s)	2579
Ksp22I	TGATCA	2 cut(s)	333, 2400
LmnI	GCTCC	8 cut(s)	82, 304, 1066, 1894, 2604, 2695, 3116, 3251
Lsp1109I	GCAGC	7 cut(s)	15, 751, 1082, 1631, 1646, 2691, 3514
MaeI	CTAG	3 cut(s)	2459, 2529, 3113
MaeII	ACGT	3 cut(s)	915, 2326, 2865
MfeI	CAATTG	2 cut(s)	1140, 2010
MflI	RGATCY	8 cut(s)	42, 523, 953, 1510, 2065, 2381, 2736, 3485
MhlI	GDGCHC	1 cut(s)	2326
MlsI	TGGCCA	1 cut(s)	1257
MluNI	TGGCCA	1 cut(s)	1257
Mly113I	GGCGCC	1 cut(s)	2580
MlyI	GAGTC	3 cut(s)	773, 1613, 2267
MmeI	TCCRAC	4 cut(s)	542, 697, 931, 2984
Mox20I	TGGCCA	1 cut(s)	1257
Mph1103I	ATGCAT	3 cut(s)	2398, 3137, 3283
MroXI	GAANNNNTTC	3 cut(s)	513, 1393, 1526
MscI	TGGCCA	1 cut(s)	1257
MslI	CAYNNNNRTG	3 cut(s)	356, 1685, 1902
Msp20I	TGGCCA	1 cut(s)	1257
MspA1I	CMGCKG	3 cut(s)	311, 2090, 2985
MspI	CCGG	3 cut(s)	137, 153, 828
MspR9I	CCNGG	3 cut(s)	105, 154, 2912
MunI	CAATTG	2 cut(s)	1140, 2010
Mva1269I	GAATGC	3 cut(s)	85, 2601, 2649
MvaI	CCWGG	2 cut(s)	105, 2912
MwoI	GCNNNNNNNGC	8 cut(s)	317, 1067, 1340, 2321, 2704, 2710, 3251, 3524
NarI	GGCGCC	1 cut(s)	2580
NciI	CCSGG	1 cut(s)	154
NcoI	CCATGG	2 cut(s)	672, 2895
NdeI	CATATG	3 cut(s)	2392, 3070, 3247
NmuCI	GTSAC	3 cut(s)	268, 2524, 3258
NsiI	ATGCAT	3 cut(s)	2398, 3137, 3283
NspI	RCATGY	4 cut(s)	1195, 1226, 1302, 3135
NspV	TTCGAA	1 cut(s)	160
OliI	CACNNNNGTG	1 cut(s)	356
PagI	TCATGA	4 cut(s)	1002, 1405, 1476, 3412
PciI	ACATGT	1 cut(s)	1191
PctI	GAATGC	3 cut(s)	85, 2601, 2649
PdmI	GAANNNNTTC	3 cut(s)	513, 1393, 1526
PfeI	GAWTC	3 cut(s)	140, 157, 469
PflFI	GACNNNGTC	1 cut(s)	1838
PinAI	ACCGGT	1 cut(s)	827
PkrI	GCNGC	9 cut(s)	30, 741, 1072, 1621, 1636, 2706, 3480, 3495, 3529
PleI	GAGTC	3 cut(s)	773, 1612, 2266
PluTI	GGCGCC	1 cut(s)	2583
PmaCI	CACGTG	1 cut(s)	2327
PmlI	CACGTG	1 cut(s)	2327
PpsI	GAGTC	3 cut(s)	773, 1612, 2266
Ppu21I	YACGTR	1 cut(s)	2327
PpuMI	RGGWCCY	1 cut(s)	688
PscI	ACATGT	1 cut(s)	1191
PsiI	TTATAA	1 cut(s)	3545
Psp5II	RGGWCCY	1 cut(s)	688
Psp6I	CCWGG	2 cut(s)	103, 2910
PspCI	CACGTG	1 cut(s)	2327
PspFI	CCCAGC	2 cut(s)	2358, 3098
PspGI	CCWGG	2 cut(s)	103, 2910
PspPI	GGNCC	3 cut(s)	101, 688, 2356
PspPPI	RGGWCCY	1 cut(s)	688
PsuI	RGATCY	8 cut(s)	42, 523, 953, 1510, 2065, 2381, 2736, 3485
PsyI	GACNNNGTC	1 cut(s)	1838
PvuII	CAGCTG	3 cut(s)	311, 2090, 2985
RsaI	GTAC	8 cut(s)	197, 707, 1196, 1231, 1733, 1812, 3128, 3437
RsaNI	GTAC	8 cut(s)	196, 706, 1195, 1230, 1732, 1811, 3127, 3436
RseI	CAYNNNNRTG	3 cut(s)	356, 1685, 1902
SatI	GCNGC	9 cut(s)	29, 740, 1071, 1620, 1635, 2705, 3479, 3494, 3528
Sau96I	GGNCC	3 cut(s)	101, 688, 2356
ScaI	AGTACT	1 cut(s)	1812
SchI	GAGTC	3 cut(s)	773, 1613, 2267
ScrFI	CCNGG	3 cut(s)	105, 154, 2912
SduI	GDGCHC	1 cut(s)	2326
SfcI	CTRYAG	1 cut(s)	2212
SfoI	GGCGCC	1 cut(s)	2581
SfuI	TTCGAA	1 cut(s)	160
SgrAI	CRCCGGYG	1 cut(s)	827
SinI	GGWCC	1 cut(s)	688
SmiI	ATTTAAAT	1 cut(s)	1902
SmiMI	CAYNNNNRTG	3 cut(s)	356, 1685, 1902
SmlI	CTYRAG	3 cut(s)	1765, 2432, 2936
SmoI	CTYRAG	3 cut(s)	1765, 2432, 2936
SsiI	CCGC	8 cut(s)	60, 1531, 2651, 3312, 3394, 3445, 3478, 3493
SspDI	GGCGCC	1 cut(s)	2579
SspI	AATATT	1 cut(s)	2690
SspMI	CTAG	3 cut(s)	2459, 2529, 3113
StyD4I	CCNGG	3 cut(s)	103, 152, 2910
StyI	CCWWGG	5 cut(s)	672, 1890, 2310, 2346, 2895
SwaI	ATTTAAAT	1 cut(s)	1902
TaaI	ACNGT	5 cut(s)	636, 2024, 2859, 3505, 3522
TaiI	ACGT	3 cut(s)	918, 2329, 2868
TaqI	TCGA	5 cut(s)	160, 385, 2130, 2907, 3297
TatI	WGTACW	6 cut(s)	195, 705, 1194, 1731, 1810, 3126
TauI	GCSGC	2 cut(s)	3481, 3496
TfiI	GAWTC	3 cut(s)	140, 157, 469
TscAI	CASTG	7 cut(s)	277, 322, 1692, 2526, 2862, 2892, 3036
TseFI	GTSAC	3 cut(s)	268, 2524, 3258
TseI	GCWGC	7 cut(s)	28, 739, 1070, 1619, 1634, 2704, 3527
Tsp45I	GTSAC	3 cut(s)	268, 2524, 3258
TspGWI	ACGGA	3 cut(s)	291, 825, 1789
TspRI	CASTG	7 cut(s)	277, 322, 1692, 2526, 2862, 2892, 3036
Tth111I	GACNNNGTC	1 cut(s)	1838
VpaK11BI	GGWCC	1 cut(s)	688
XapI	RAATTY	8 cut(s)	509, 700, 1038, 1523, 1655, 1754, 1988, 2251
XbaI	TCTAGA	1 cut(s)	2458
XceI	RCATGY	4 cut(s)	1195, 1226, 1302, 3135
XmnI	GAANNNNTTC	3 cut(s)	513, 1393, 1526
XspI	CTAG	3 cut(s)	2459, 2529, 3113
ZrmI	AGTACT	1 cut(s)	1812
Zsp2I	ATGCAT	3 cut(s)	2398, 3137, 3283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.