Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002683

Physical Location & Seq

Genomic Coordinates Reverse (-)

36265878 .. 36269057

Gene Structure 3180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1231600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGCATTCCCCAAGCCAAAGGCTGTCCCTTTTCCTCTTCTCCCTGCTGGTTTTGCTCCCCATTGCAAGACCAGATCTGGCTTCTGACCGCGCCGCTCTGCTAGCCCTGCGCTCCGCCGTCGGCGGCCGAACTCTGCTATGGGATGTGAGCCAGACCAGCCCATGTTTGTGGACAGGCGTCAGCTGTGAGAACAACCGCGTCACGGTGCTTCGTCTTCCCGGCGTGGCTCTCTCCGGCATAATCCCCAGTGGCATTTTCGGAAATCTGACTAGTCTCCGCACTCTCAGTCTCCGTCTCAACGCTTTAAGAGGTCCTCTGCCTTCAGATCTCTCTGCCTGCGTTACTCTTCGTAACCTTTACTTGCAGGGCAACCTTTTCTCCGGCGAAATCCCGGAGTTTGTGTACAGTCTGCACGACTTGGTCCGGCTGAACTTGGCCTCCAACAATTTTTCCGGTGAGATCTCTCTGGGTTTCAACAATCTCACCAGGCTCAGGACTTTGTATCTCGAAAGCAACAAGCTTTCGGGGGCGATCCCGGAGCTGAAGCTGCCCAATCTCGACCAGTTCAATGTATCCAACAATTTGCTAAACGGGTCTGTTCCAAAACAGTTGCAGTCTTACTCTTCGAGCTCGTTTCTGGGTAATTCTCTATGCGGGCGTCCCCTTAATGCTTGTCCTGGAGACCGTGGCGGAGCTGCCAATCCTGCCATAGGAGGGGATATAAACATCAATGACCACCACAAAAAGAGAAAGCTTTCCGGTGGTGCTATTGCCGGTATTGTGATCGGATCTGTGTTGGCTTTTCTCGTGATTGTTATGTTTTTGATCTTTTTCTGCCGAAAGAAGAAGAGCAAGAAGACGAGCTCCGTCGACATTGCCACCGTGAAGCACCGGGAAGTCGAGATTCCTGGCGAGAAGCTGCCCGTGGAGTCTGAGAATGGAGGATATGGAAATGGGCATTCGGTGGCGGATGCTGCCGCTGCGGCGATGGTGGGGAATGGCAAAAGTGAAGCCGGTGGCGCTAGTGGTGCTAAAAAGCTGGCCTTTTTTGGCAATGCGGCGAGGGTGTTTGATCTGGAGGACTTGTTGAGAGCTTCGGCGGAGGTTTTGGGGAAGGGGACTTTTGGGACAGCGTACAAAGCGGTTCTGGAGGCTGGGACTGTGGTGGCTGTGAAGAGGCTGAAGGACGTGACGATTTCTGAGAGCGAGTTTAAGGAAAAGATTGAGGCTGTGGGAGCGAAGGATCATGAGAATTTGGTGCCTCTGAGGGCATATTATTTCAGCAGAGATGAGAAGCTTCTTGTCTATGATTACATGCCTATGGGAAGCTTATCTGCACTTTTGCATGGAAACAAAGGAGCAGGTAGGACTCCATTGAATTGGGAAATCAGGTCTGGCATTGCCCTTGGAGCTGCCCGTGGAATTGAATACCTACACTCTCAAGGTCATACTGTCTCCCACGGAAACATAAAGTCATCCAACATCCTGCTCACAAAGTCCTATGAAGCTCGGGTTTCTGACTTTGGCCTAGCGCACCTTGTTGGCCCCTCATCCACTCCCAACCGAGTTGCTGGCTACCGGGCACCGGAGGTTACTGATCCTCGCAAGGTATCCCAAAAGGCCGATGTTTATAGCTTTGGGGTGTTGCTCCTGGAGCTACTCACCGGAAAGCCCCCAACGCATGCCCTCTTGAATGAGGAAGGAGTTGACCTCCCAAGATGGGTTCAGTCCATAGTCAAAGAGGAGTGGACTTCAGAGGTTTTCGATCTTGAGCTCCTCAGGTACCAGAATGTCGAGGAGGAGATGGTTCAGCTCTTGCAACTTGCAATAGATTGTTCAGCCCAGTACCCTGACAAACGCCCTTCAATCTCCGAAGTTACAAGGCGCATTGAGGAGCTACGTCGTTCTAGCTTGCTAGATGAGCAGCCCGAGGTTGTCCATGATTTGGACGATGTGTCTTCTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

656

Amino Acids

70.98

Weight (kDa)

8.3

Isoelectric Point (pI)

40.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	27 - 62	1.3e-08	Leucine rich repeat N-terminal domain
Pkinase	PF00069	368 - 627	3.2e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	368 - 629	3.8e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48480
fragaria_vesca	FvH4_2g12190 FvH4_2g12190 FvH4_5g17570
malus_domestica	MD02G1259100.v1.1 MD06G1231600.v1.1 MD09G1086600.v1.1 MD09G1271200.v1.1 MD14G1238300.v1.1 MD17G1269300.v1.1
prunus_persica	Prupe.3G017300_v2.0.a1 Prupe.5G235500_v2.0.a1
pyrus_communis	pycom06g20550 pycom09g18220 pycom14g19950 pycom17g26800
rosa_chinensis	RchiOBHm_Chr3g0491091 RchiOBHm_Chr7g0178081
rosa_laevigata	RLG00000005473 RLG00000022865
rosa_multiflora	Rmu_co8170494.1_g000001 Rmu_co8379533.1_g000001 Rmu_co8445967.1_g000001 Rmu_sc0000425.1_g000007 Rmu_sc0005038.1_g000014 Rmu_ssc0000386.1_g000061 Rmu_ssc0000386.1_g000063
rosa_roxburghii	Rroxscaffold_6G00393220
rosa_rugosa	Rorug03G0246000 Rorug06G0413300
rosa_samantha	Rh3AG296200 Rh3BG332200 Rh3CG329300 Rh3DG329900 Rh7AG012600 Rh7BG012500 Rh7CG012500 Rh7DG012800
rosa_wichuraiana	Rw3G026140 Rw7G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1352
Acc65I	GGTACC	1 cut(s)	1782
AccB1I	GGYRCC	3 cut(s)	1258, 1582, 1782
AccB7I	CCANNNNNTGG	1 cut(s)	1945
AccBSI	CCGCTC	1 cut(s)	95
AccI	GTMKAC	1 cut(s)	870
AccII	CGCG	2 cut(s)	90, 198
AclWI	GGATC	4 cut(s)	526, 796, 1251, 1592
AcoI	YGGCCR	1 cut(s)	124
AcsI	RAATTY	1 cut(s)	1252
AcuI	CTGAAG	4 cut(s)	306, 563, 1202, 1737
AcyI	GRCGYC	2 cut(s)	177, 658
AfaI	GTAC	4 cut(s)	404, 1136, 1784, 1847
AfiI	CCNNNNNNNGG	7 cut(s)	202, 492, 710, 714, 1585, 1720, 1945
AgsI	TTSAA	6 cut(s)	475, 568, 1378, 1427, 1693, 1866
AhdI	GACNNNNNGTC	1 cut(s)	1954
AhlI	ACTAGT	1 cut(s)	269
AjiI	CACGTC	1 cut(s)	1189
AjnI	CCWGG	4 cut(s)	485, 676, 907, 1650
AloI	GAACNNNNNNTCC	2 cut(s)	422, 454
Alw21I	GWGCWC	3 cut(s)	632, 866, 1776
Alw26I	GTCTC	5 cut(s)	278, 293, 299, 675, 1459
AlwI	GGATC	4 cut(s)	526, 796, 1251, 1592
Ama87I	CYCGRG	2 cut(s)	1509, 1928
AoxI	GGCC	6 cut(s)	124, 435, 1041, 1525, 1543, 1620
ApeKI	GCWGC	7 cut(s)	547, 695, 919, 974, 980, 1412, 1924
ApoI	RAATTY	1 cut(s)	1252
Asp700I	GAANNNNTTC	1 cut(s)	754
Asp718I	GGTACC	1 cut(s)	1782
AspLEI	GCGC	5 cut(s)	92, 111, 1022, 1534, 1887
AspS9I	GGNCC	3 cut(s)	311, 421, 1544
AsuC2I	CCSGG	5 cut(s)	219, 392, 536, 893, 1580
AsuHPI	GGTGA	3 cut(s)	467, 475, 1654
AsuNHI	GCTAGC	1 cut(s)	100
AvaI	CYCGRG	2 cut(s)	1509, 1928
AvaII	GGWCC	2 cut(s)	311, 421
AxyI	CCTNAGG	1 cut(s)	1778
BaeGI	GKGCMC	1 cut(s)	1585
BanI	GGYRCC	3 cut(s)	1258, 1582, 1782
BanII	GRGCYC	3 cut(s)	632, 866, 1776
BauI	CACGAG	1 cut(s)	806
BbsI	GAAGAC	3 cut(s)	206, 863, 1950
Bbv12I	GWGCWC	3 cut(s)	632, 866, 1776
BbvI	GCAGC	7 cut(s)	534, 682, 906, 961, 967, 1399, 1936
BccI	CCATC	3 cut(s)	982, 1713, 1798
BceAI	ACGGC	1 cut(s)	101
BciT130I	CCWGG	4 cut(s)	487, 678, 909, 1652
BciVI	GTATCC	2 cut(s)	583, 1621
BcnI	CCSGG	5 cut(s)	219, 392, 536, 893, 1580
BcoDI	GTCTC	5 cut(s)	278, 293, 299, 675, 1459
BcuI	ACTAGT	1 cut(s)	269
BfaI	CTAG	6 cut(s)	101, 270, 1023, 1529, 1908, 1916
BfoI	RGCGCY	1 cut(s)	1023
BfuAI	ACCTGC	1 cut(s)	1352
BfuI	GTATCC	2 cut(s)	583, 1621
BglI	GCCNNNNNGGC	1 cut(s)	983
BglII	AGATCT	3 cut(s)	73, 325, 459
Bme1390I	CCNGG	9 cut(s)	219, 392, 487, 536, 678, 893, 909, 1580, 1652
Bme18I	GGWCC	2 cut(s)	311, 421
BmeRI	GACNNNNNGTC	1 cut(s)	1954
BmeT110I	CYCGRG	2 cut(s)	1509, 1928
BmgBI	CACGTC	1 cut(s)	1189
BmgT120I	GGNCC	3 cut(s)	311, 421, 1544
BmiI	GGNNCC	4 cut(s)	1260, 1546, 1584, 1784
BmrFI	CCNGG	9 cut(s)	219, 392, 487, 536, 678, 893, 909, 1580, 1652
BmrI	ACTGGG	2 cut(s)	240, 1837
BmsI	GCATC	1 cut(s)	961
BmtI	GCTAGC	1 cut(s)	104
BmuI	ACTGGG	2 cut(s)	240, 1837
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	3 cut(s)	206, 863, 1950
BplI	GAGNNNNNCTC	2 cut(s)	1695, 1727
BpmI	CTGGAG	4 cut(s)	699, 1097, 1169, 1673
Bpu10I	CCTNAGC	1 cut(s)	491
BpuEI	CTTGAG	2 cut(s)	1425, 1790
BpuMI	CCSGG	5 cut(s)	219, 392, 536, 893, 1580
BsaHI	GRCGYC	2 cut(s)	177, 658
BsaI	GGTCTC	1 cut(s)	675
BsaJI	CCNNGG	6 cut(s)	685, 924, 1405, 1417, 1459, 1929
BsaWI	WCCGGW	4 cut(s)	452, 758, 1585, 1664
BsaXI	ACNNNNNCTCC	8 cut(s)	362, 386, 392, 416, 422, 452, 1070, 1100
Bsc4I	CCNNNNNNNGG	7 cut(s)	202, 492, 710, 714, 1585, 1720, 1945
Bse118I	RCCGGY	2 cut(s)	773, 1013
Bse1I	ACTGG	3 cut(s)	246, 562, 1843
Bse21I	CCTNAGG	1 cut(s)	1778
Bse3DI	GCAATG	4 cut(s)	60, 873, 1060, 1398
BseBI	CCWGG	4 cut(s)	487, 678, 909, 1652
BseDI	CCNNGG	6 cut(s)	685, 924, 1405, 1417, 1459, 1929
BseGI	GGATG	5 cut(s)	148, 976, 1475, 1482, 1550
BseLI	CCNNNNNNNGG	7 cut(s)	202, 492, 710, 714, 1585, 1720, 1945
BseMI	GCAATG	4 cut(s)	60, 873, 1060, 1398
BseMII	CTCAG	6 cut(s)	298, 505, 924, 1191, 1256, 1792
BseNI	ACTGG	3 cut(s)	246, 562, 1843
BseRI	GAGGAG	5 cut(s)	1757, 1766, 1811, 1814, 1907
BseSI	GKGCMC	1 cut(s)	1585
BseX3I	CGGCCG	1 cut(s)	124
BseXI	GCAGC	7 cut(s)	534, 682, 906, 961, 967, 1399, 1936
BseYI	CCCAGC	1 cut(s)	1154
BsgI	GTGCAG	2 cut(s)	395, 1320
Bsh1236I	CGCG	2 cut(s)	90, 198
Bsh1285I	CGRYCG	1 cut(s)	127
BshFI	GGCC	6 cut(s)	126, 437, 1043, 1527, 1545, 1622
BshNI	GGYRCC	3 cut(s)	1258, 1582, 1782
BsiEI	CGRYCG	1 cut(s)	127
BsiHKAI	GWGCWC	3 cut(s)	632, 866, 1776
BsiHKCI	CYCGRG	2 cut(s)	1509, 1928
BslFI	GGGAC	5 cut(s)	10, 645, 1132, 1141, 1171
BslI	CCNNNNNNNGG	7 cut(s)	202, 492, 710, 714, 1585, 1720, 1945
BsmAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1459
BsmBI	CGTCTC	1 cut(s)	299
BsmFI	GGGAC	5 cut(s)	10, 645, 1132, 1141, 1171
BsmI	GAATGC	2 cut(s)	4, 958
BsnI	GGCC	6 cut(s)	126, 437, 1043, 1527, 1545, 1622
Bso31I	GGTCTC	1 cut(s)	675
BsoBI	CYCGRG	2 cut(s)	1509, 1928
Bsp1286I	GDGCHC	4 cut(s)	632, 866, 1585, 1776
Bsp1407I	TGTACA	1 cut(s)	402
BspANI	GGCC	6 cut(s)	126, 437, 1043, 1527, 1545, 1622
BspCNI	CTCAG	6 cut(s)	297, 504, 925, 1192, 1257, 1791
BspFNI	CGCG	2 cut(s)	90, 198
BspHI	TCATGA	1 cut(s)	1246
BspLI	GGNNCC	4 cut(s)	1260, 1546, 1584, 1784
BspMI	ACCTGC	1 cut(s)	1352
BspOI	GCTAGC	1 cut(s)	104
BspPI	GGATC	4 cut(s)	526, 796, 1251, 1592
BspQI	GCTCTTC	1 cut(s)	842
BspT107I	GGYRCC	3 cut(s)	1258, 1582, 1782
BspTNI	GGTCTC	1 cut(s)	675
BsrBI	CCGCTC	1 cut(s)	95
BsrDI	GCAATG	4 cut(s)	60, 873, 1060, 1398
BsrFI	RCCGGY	2 cut(s)	773, 1013
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	3 cut(s)	246, 562, 1843
BssAI	RCCGGY	2 cut(s)	773, 1013
BssECI	CCNNGG	6 cut(s)	685, 924, 1405, 1417, 1459, 1929
BssNI	GRCGYC	2 cut(s)	177, 658
BssSI	CACGAG	1 cut(s)	806
BssT1I	CCWWGG	1 cut(s)	1405
Bst2BI	CACGAG	1 cut(s)	806
Bst2UI	CCWGG	4 cut(s)	487, 678, 909, 1652
Bst4CI	ACNGT	7 cut(s)	205, 407, 609, 686, 883, 1162, 1453
Bst6I	CTCTTC	5 cut(s)	41, 351, 628, 842, 1169
BstACI	GRCGYC	2 cut(s)	177, 658
BstAUI	TGTACA	1 cut(s)	402
BstC8I	GCNNGC	7 cut(s)	102, 337, 656, 1041, 1573, 1683, 1913
BstDEI	CTNAG	6 cut(s)	284, 491, 933, 1200, 1265, 1778
BstDSI	CCRYGG	4 cut(s)	685, 924, 1417, 1459
BstENI	CCTNNNNNAGG	1 cut(s)	708
BstF5I	GGATG	5 cut(s)	148, 976, 1475, 1482, 1550
BstFNI	CGCG	2 cut(s)	90, 198
BstH2I	RGCGCY	1 cut(s)	1023
BstHHI	GCGC	5 cut(s)	92, 111, 1022, 1534, 1887
BstMAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1459
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	4 cut(s)	487, 678, 909, 1652
BstNSI	RCATGY	2 cut(s)	1318, 1685
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	9 cut(s)	217, 390, 485, 534, 676, 891, 907, 1578, 1650
BstSLI	GKGCMC	1 cut(s)	1585
BstUI	CGCG	2 cut(s)	90, 198
BstV1I	GCAGC	7 cut(s)	534, 682, 906, 961, 967, 1399, 1936
BstV2I	GAAGAC	3 cut(s)	206, 863, 1950
BstX2I	RGATCY	4 cut(s)	73, 325, 459, 788
BstXI	CCANNNNNNTGG	2 cut(s)	168, 1380
BstYI	RGATCY	4 cut(s)	73, 325, 459, 788
BstZI	CGGCCG	1 cut(s)	124
Bsu36I	CCTNAGG	1 cut(s)	1778
BsuI	GTATCC	2 cut(s)	583, 1621
BsuRI	GGCC	6 cut(s)	126, 437, 1043, 1527, 1545, 1622
BtgI	CCRYGG	4 cut(s)	685, 924, 1417, 1459
BtgZI	GCGATG	1 cut(s)	1001
BtrI	CACGTC	1 cut(s)	1189
BtsCI	GGATG	5 cut(s)	148, 976, 1475, 1482, 1550
BtsIMutI	CAGTG	1 cut(s)	253
BveI	ACCTGC	1 cut(s)	1352
Cac8I	GCNNGC	7 cut(s)	102, 337, 656, 1041, 1573, 1683, 1913
CciI	TCATGA	1 cut(s)	1246
CfoI	GCGC	5 cut(s)	92, 111, 1022, 1534, 1887
Cfr10I	RCCGGY	2 cut(s)	773, 1013
Cfr13I	GGNCC	3 cut(s)	311, 421, 1544
CseI	GACGC	3 cut(s)	166, 187, 647
Csp6I	GTAC	4 cut(s)	403, 1135, 1783, 1846
CviAII	CATG	6 cut(s)	162, 1247, 1315, 1346, 1682, 1940
CviQI	GTAC	4 cut(s)	403, 1135, 1783, 1846
DdeI	CTNAG	6 cut(s)	284, 491, 933, 1200, 1265, 1778
DriI	GACNNNNNGTC	1 cut(s)	1954
EaeI	YGGCCR	1 cut(s)	124
EagI	CGGCCG	1 cut(s)	124
Eam1104I	CTCTTC	5 cut(s)	41, 351, 628, 842, 1169
Eam1105I	GACNNNNNGTC	1 cut(s)	1954
EarI	CTCTTC	5 cut(s)	41, 351, 628, 842, 1169
EciI	GGCGGA	4 cut(s)	103, 705, 983, 1115
Ecl136II	GAGCTC	3 cut(s)	630, 864, 1774
EclXI	CGGCCG	1 cut(s)	124
Eco130I	CCWWGG	1 cut(s)	1405
Eco24I	GRGCYC	3 cut(s)	632, 866, 1776
Eco31I	GGTCTC	1 cut(s)	675
Eco47I	GGWCC	2 cut(s)	311, 421
Eco52I	CGGCCG	1 cut(s)	124
Eco53kI	GAGCTC	3 cut(s)	630, 864, 1774
Eco57I	CTGAAG	4 cut(s)	306, 563, 1202, 1737
Eco81I	CCTNAGG	1 cut(s)	1778
Eco88I	CYCGRG	2 cut(s)	1509, 1928
EcoICRI	GAGCTC	3 cut(s)	630, 864, 1774
EcoNI	CCTNNNNNAGG	1 cut(s)	708
EcoO109I	RGGNCCY	1 cut(s)	311
EcoRII	CCWGG	4 cut(s)	485, 676, 907, 1650
EcoT14I	CCWWGG	1 cut(s)	1405
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	632, 866, 1776
ErhI	CCWWGG	1 cut(s)	1405
Esp3I	CGTCTC	1 cut(s)	299
FaeI	CATG	6 cut(s)	165, 1250, 1318, 1349, 1685, 1943
FalI	AAGNNNNNCTT	2 cut(s)	1028, 1060
FaqI	GGGAC	5 cut(s)	10, 645, 1132, 1141, 1171
FatI	CATG	6 cut(s)	161, 1246, 1314, 1345, 1681, 1939
FauI	CCCGC	1 cut(s)	647
FblI	GTMKAC	1 cut(s)	870
FokI	GGATG	5 cut(s)	155, 983, 1462, 1469, 1537
FriOI	GRGCYC	3 cut(s)	632, 866, 1776
FspBI	CTAG	6 cut(s)	101, 270, 1023, 1529, 1908, 1916
GlaI	GCGC	5 cut(s)	91, 110, 1021, 1533, 1886
GsaI	CCCAGC	1 cut(s)	1158
GsuI	CTGGAG	4 cut(s)	699, 1097, 1169, 1673
HaeII	RGCGCY	1 cut(s)	1023
HaeIII	GGCC	6 cut(s)	126, 437, 1043, 1527, 1545, 1622
HgaI	GACGC	3 cut(s)	166, 187, 647
HhaI	GCGC	5 cut(s)	92, 111, 1022, 1534, 1887
Hin1I	GRCGYC	2 cut(s)	177, 658
Hin1II	CATG	6 cut(s)	165, 1250, 1318, 1349, 1685, 1943
Hin6I	GCGC	5 cut(s)	90, 109, 1020, 1532, 1885
HinP1I	GCGC	5 cut(s)	90, 109, 1020, 1532, 1885
HincII	GTYRAC	2 cut(s)	871, 1708
HindII	GTYRAC	2 cut(s)	871, 1708
HindIII	AAGCTT	4 cut(s)	518, 752, 1295, 1327
HinfI	GANTC	3 cut(s)	904, 929, 1369
HphI	GGTGA	3 cut(s)	467, 475, 1654
Hpy166II	GTNNAC	5 cut(s)	171, 403, 871, 1708, 1749
Hpy8I	GTNNAC	5 cut(s)	171, 403, 871, 1708, 1749
Hpy99I	CGWCG	3 cut(s)	122, 872, 1905
HpyAV	CCTTC	6 cut(s)	330, 1108, 1177, 1234, 1694, 1872
HpyCH4III	ACNGT	7 cut(s)	205, 407, 609, 686, 883, 1162, 1453
HpyCH4IV	ACGT	2 cut(s)	1188, 1900
HpyCH4V	TGCA	9 cut(s)	4, 65, 364, 412, 613, 1337, 1345, 1819, 1826
HpyF3I	CTNAG	6 cut(s)	284, 491, 933, 1200, 1265, 1778
HpySE526I	ACGT	2 cut(s)	1188, 1900
Hsp92I	GRCGYC	2 cut(s)	177, 658
Hsp92II	CATG	6 cut(s)	165, 1250, 1318, 1349, 1685, 1943
HspAI	GCGC	5 cut(s)	90, 109, 1020, 1532, 1885
KpnI	GGTACC	1 cut(s)	1786
LguI	GCTCTTC	1 cut(s)	842
Lsp1109I	GCAGC	7 cut(s)	534, 682, 906, 961, 967, 1399, 1936
LweI	GCATC	1 cut(s)	961
MaeI	CTAG	6 cut(s)	101, 270, 1023, 1529, 1908, 1916
MaeII	ACGT	2 cut(s)	1188, 1900
MaeIII	GTNAC	6 cut(s)	199, 340, 350, 1189, 1591, 1876
MbiI	CCGCTC	1 cut(s)	95
MboII	GAAGA	9 cut(s)	28, 206, 338, 615, 856, 859, 868, 1186, 1950
MflI	RGATCY	4 cut(s)	73, 325, 459, 788
MhlI	GDGCHC	4 cut(s)	632, 866, 1585, 1776
MluCI	AATT	6 cut(s)	445, 580, 643, 1252, 1378, 1422
MlyI	GAGTC	2 cut(s)	938, 1363
MmeI	TCCRAC	3 cut(s)	465, 600, 1503
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	754
MseI	TTAA	3 cut(s)	305, 666, 1212
MslI	CAYNNNNRTG	2 cut(s)	166, 1319
MspA1I	CMGCKG	2 cut(s)	183, 980
MspR9I	CCNGG	9 cut(s)	219, 392, 487, 536, 678, 893, 909, 1580, 1652
Mva1269I	GAATGC	2 cut(s)	4, 958
MvaI	CCWGG	4 cut(s)	487, 678, 909, 1652
MvnI	CGCG	2 cut(s)	90, 198
NciI	CCSGG	5 cut(s)	219, 392, 536, 893, 1580
NheI	GCTAGC	1 cut(s)	100
NlaIII	CATG	6 cut(s)	165, 1250, 1318, 1349, 1685, 1943
NlaIV	GGNNCC	4 cut(s)	1260, 1546, 1584, 1784
NmuCI	GTSAC	2 cut(s)	199, 1189
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	1318, 1685
PaeI	GCATGC	1 cut(s)	1685
PagI	TCATGA	1 cut(s)	1246
PciSI	GCTCTTC	1 cut(s)	842
PctI	GAATGC	2 cut(s)	4, 958
PdmI	GAANNNNTTC	1 cut(s)	754
PfeI	GAWTC	1 cut(s)	904
PflFI	GACNNNGTC	1 cut(s)	419
PflMI	CCANNNNNTGG	1 cut(s)	1945
PfoI	TCCNGGA	4 cut(s)	390, 534, 676, 1650
PleI	GAGTC	2 cut(s)	937, 1363
PpsI	GAGTC	2 cut(s)	937, 1363
PpuMI	RGGWCCY	1 cut(s)	311
PshAI	GACNNNNGTC	1 cut(s)	176
Psp124BI	GAGCTC	3 cut(s)	632, 866, 1776
Psp5II	RGGWCCY	1 cut(s)	311
Psp6I	CCWGG	4 cut(s)	485, 676, 907, 1650
PspFI	CCCAGC	1 cut(s)	1154
PspGI	CCWGG	4 cut(s)	485, 676, 907, 1650
PspN4I	GGNNCC	4 cut(s)	1260, 1546, 1584, 1784
PspPI	GGNCC	3 cut(s)	311, 421, 1544
PspPPI	RGGWCCY	1 cut(s)	311
PsuI	RGATCY	4 cut(s)	73, 325, 459, 788
PsyI	GACNNNGTC	1 cut(s)	419
PvuII	CAGCTG	1 cut(s)	183
RsaI	GTAC	4 cut(s)	404, 1136, 1784, 1847
RsaNI	GTAC	4 cut(s)	403, 1135, 1783, 1846
RseI	CAYNNNNRTG	2 cut(s)	166, 1319
SacI	GAGCTC	3 cut(s)	632, 866, 1776
SalI	GTCGAC	1 cut(s)	869
SapI	GCTCTTC	1 cut(s)	842
SaqAI	TTAA	3 cut(s)	305, 666, 1212
Sau96I	GGNCC	3 cut(s)	311, 421, 1544
SchI	GAGTC	2 cut(s)	938, 1363
ScrFI	CCNGG	9 cut(s)	219, 392, 487, 536, 678, 893, 909, 1580, 1652
SduI	GDGCHC	4 cut(s)	632, 866, 1585, 1776
SfaNI	GCATC	1 cut(s)	961
SinI	GGWCC	2 cut(s)	311, 421
SmiMI	CAYNNNNRTG	2 cut(s)	166, 1319
SmlI	CTYRAG	2 cut(s)	1440, 1769
SmoI	CTYRAG	2 cut(s)	1440, 1769
SpeI	ACTAGT	1 cut(s)	269
SphI	GCATGC	1 cut(s)	1685
Sse9I	AATT	6 cut(s)	445, 580, 643, 1252, 1378, 1422
SspMI	CTAG	6 cut(s)	101, 270, 1023, 1529, 1908, 1916
SstI	GAGCTC	3 cut(s)	632, 866, 1776
StyD4I	CCNGG	9 cut(s)	217, 390, 485, 534, 676, 891, 907, 1578, 1650
StyI	CCWWGG	1 cut(s)	1405
TaaI	ACNGT	7 cut(s)	205, 407, 609, 686, 883, 1162, 1453
TaiI	ACGT	2 cut(s)	1191, 1903
TaqI	TCGA	8 cut(s)	507, 558, 626, 870, 900, 1764, 1794, 1963
TasI	AATT	6 cut(s)	445, 580, 643, 1252, 1378, 1422
TatI	WGTACW	1 cut(s)	402
TauI	GCSGC	5 cut(s)	95, 126, 980, 986, 1061
TfiI	GAWTC	1 cut(s)	904
Tru1I	TTAA	3 cut(s)	305, 666, 1212
Tru9I	TTAA	3 cut(s)	305, 666, 1212
TscAI	CASTG	1 cut(s)	253
TseFI	GTSAC	2 cut(s)	199, 1189
TseI	GCWGC	7 cut(s)	547, 695, 919, 974, 980, 1412, 1924
Tsp45I	GTSAC	2 cut(s)	199, 1189
TspDTI	ATGAA	1 cut(s)	1518
TspGWI	ACGGA	3 cut(s)	281, 856, 1476
TspRI	CASTG	1 cut(s)	253
Tth111I	GACNNNGTC	1 cut(s)	419
Van91I	CCANNNNNTGG	1 cut(s)	1945
VpaK11BI	GGWCC	2 cut(s)	311, 421
XagI	CCTNNNNNAGG	1 cut(s)	708
XapI	RAATTY	1 cut(s)	1252
XceI	RCATGY	2 cut(s)	1318, 1685
XmiI	GTMKAC	1 cut(s)	870
XmnI	GAANNNNTTC	1 cut(s)	754
XspI	CTAG	6 cut(s)	101, 270, 1023, 1529, 1908, 1916
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.