Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0002683

Physical Location & Seq

Genomic Coordinates Reverse (-)

21108037 .. 21110476

Gene Structure 2440 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g19950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGCATTCCCAAACCCAAAGGCTTTGCCTTTTCCTCTCCTCCCTGCTGCTTTTGCTCCCCATTGCAAAACCAGATCTGGCTTCTGACCGCGCCGCTCTACTGGCCCTGCGTTCCGCCGTCGGCGGCCGAACTCTGTTTTGGAATGTGAACCAGACCAGCCCATGTTCGTGGGCTGGCGTCAACTGTGAGGACAACCGTGTCACGGGGATTCGTCTTCCCGGCGTGGCTCTATCCGGCGTAATCCCGAGTGGCATTTTTGGAAATCTGACTAGTCTCCGCACTCTCAGTCTCCGTCTCAACGCTTTAAGGGGTCCTCTGCCTTCCGATCTCTCCGCCTGTGTTACTCTTCGTAACCTTTACTTGCAGGGGAACCTTTTCTCCGGCGAAATCCCGGAGTTTTTGTACAGTCTACACGACTTGGTCCGGCTGAACTTGGCGTCCAACAATTTTTCCGGCGAGATCTCACTGGGTTTCAACAATCTCACCCGCCTAAGGACTTTGTATCTTGAAAGCAACAAGCTTTCTGGAGCGATTCCTGAGCTGAAGCTTCCTAATCTCGACCAGTTTAATGTATCCAACAATTTGCTAAACGGGTCTGTTCCGAAGCAGTTGCAGTCTTACTCTTCAAGCTCGTTTCTGGGTAATTCGCTATGCGGGCTTCCGCTTGATGCTTGCCCTGGAGACGGCGGAGGAGATATAAACATGAATGACAGCCACAAAGGGAGAAAGCTTTCAGGTGGTGCTATTGCCGGTATTGTGATCGGATCCGTGCTGTCTTTTCTCGTGATTGTCATGCTTTTGATATTTTTCTGCCGAAAGAAGAAGAGCAAGAAGACGAGCTCCGTCGACATCGCCACCGTGAAGCACCCGGATGTCGAGATTCCCGGCGAGAAGCTGCCCGCGGAGGCTGAAAATGTGGGATACGGAAATGGGTCTTCGGTGGCGGCTGCTGCGGCGGCGGCGATGGTGGGGAATGGGAAAAGTGAAGCCAATAGCGCCGTTGGTGCTAAAAAGCTGGTGTTTTTTGGCAATGGGGCGAGGGTGTTTGATCTGGAGGACTTGTTGAGAGCTTCGGCGGAGGTTTTGGGGAAGGGGACTTTCGGGACCGCATACAAGGCGGTTCTGGAGGCTGGGACTGTGGTGGCCGTGAAGAGGCTGAGGGATGTGACAATTTCGGAGAGCGAGTTTAAAGAAAAGATTGAAGCTGTGGGAGTGAAGGATCATGAAACTTTGGTGCCCCTGAGGGCCTACTATTTCAGCAGAGATGAGAAGCTTCTTGTCTATGATTACATGCCTATGGGAAGCTTATCTGCACTTTTGCACGGAAACAAAGGAGCAGGCAGGACCCCATTGAATTGGGAAATCAGGTCCAGAATTGCCCTTGGAGCTGCCCGTGGAATTGAATACCTACACTCTCAAGGCCAAACTGTCTCGCATGGAAACATCAAGTCATCCAACATCCTGCTAACAAAGTCCTATGAAGCTCGGGTTTCTGACTTCGGCCTAGCACACCTTGTTGGTCCCTCATCCACTCCCAACCGAGTTTCTGGCTACCGTGCACCAGAGGTTACTGATCCTCGCAAGGTATCCCAAAAGGCCGATGTTTATAGCTTTGGGGTATTGCTCTTGGAGCTACTTACTGGGAAGCCCCCAACTCAAGCGCTCTTGAATGAGGAAGGAGTTGACCTCCCAAGGTGGGTTCAGTCCATAGTCAAAGAGGAGTGGACTTCAGAGGTTTTCGATGTTGAGCTCCTCAGGTACCAGAATGTCGAGGAGGAGATGGTTCAGCTCTTGCAACTTGCAATAGATTGTTCGGCTCAGTATCCTGACAAACGCCCTTCAATCTCCGAAGTGACAAGGCGCATTGAGGAGCTACGTCATTCTAGCTTGCGAGATGAGCAGCCCGCGGTTGTCCGTGGTTTAGACGATGTGTCCTCTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

648

Amino Acids

70.04

Weight (kDa)

8.27

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	27 - 62	1.5e-08	Leucine rich repeat N-terminal domain
Pkinase	PF00069	360 - 619	7.8e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	360 - 621	5.5e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48480
fragaria_vesca	FvH4_2g12190 FvH4_2g12190 FvH4_5g17570
malus_domestica	MD02G1259100.v1.1 MD06G1231600.v1.1 MD09G1086600.v1.1 MD09G1271200.v1.1 MD14G1238300.v1.1 MD17G1269300.v1.1
prunus_persica	Prupe.3G017300_v2.0.a1 Prupe.5G235500_v2.0.a1
pyrus_communis	pycom06g20550 pycom09g18220 pycom14g19950 pycom17g26800
rosa_chinensis	RchiOBHm_Chr3g0491091 RchiOBHm_Chr7g0178081
rosa_laevigata	RLG00000005473 RLG00000022865
rosa_multiflora	Rmu_co8170494.1_g000001 Rmu_co8379533.1_g000001 Rmu_co8445967.1_g000001 Rmu_sc0000425.1_g000007 Rmu_sc0005038.1_g000014 Rmu_ssc0000386.1_g000061 Rmu_ssc0000386.1_g000063
rosa_roxburghii	Rroxscaffold_6G00393220
rosa_rugosa	Rorug03G0246000 Rorug06G0413300
rosa_samantha	Rh3AG296200 Rh3BG332200 Rh3CG329300 Rh3DG329900 Rh7AG012600 Rh7BG012500 Rh7CG012500 Rh7DG012800
rosa_wichuraiana	Rw3G026140 Rw7G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	197
Acc65I	GGTACC	1 cut(s)	1758
AccB1I	GGYRCC	2 cut(s)	1234, 1758
AccBSI	CCGCTC	1 cut(s)	95
AccI	GTMKAC	2 cut(s)	409, 846
AccII	CGCG	3 cut(s)	90, 902, 1907
AclWI	GGATC	4 cut(s)	759, 772, 1227, 1568
AcoI	YGGCCR	2 cut(s)	124, 1143
AcuI	CTGAAG	2 cut(s)	563, 1713
AcyI	GRCGYC	2 cut(s)	177, 437
AfaI	GTAC	2 cut(s)	404, 1760
AfeI	AGCGCT	1 cut(s)	1662
AfiI	CCNNNNNNNGG	5 cut(s)	202, 492, 683, 1152, 1696
AgsI	TTSAA	8 cut(s)	475, 509, 627, 1202, 1354, 1403, 1669, 1842
AhdI	GACNNNNNGTC	1 cut(s)	1930
AhlI	ACTAGT	1 cut(s)	269
AjnI	CCWGG	1 cut(s)	676
AjuI	GAANNNNNNNTTGG	2 cut(s)	121, 153
AloI	GAACNNNNNNTCC	4 cut(s)	362, 394, 422, 454
Alw21I	GWGCWC	3 cut(s)	842, 1561, 1752
Alw26I	GTCTC	5 cut(s)	278, 293, 299, 675, 1435
Alw44I	GTGCAC	1 cut(s)	1557
AlwI	GGATC	4 cut(s)	759, 772, 1227, 1568
Ama87I	CYCGRG	2 cut(s)	244, 1485
Aor51HI	AGCGCT	1 cut(s)	1662
AoxI	GGCC	7 cut(s)	102, 124, 1143, 1245, 1420, 1501, 1596
ApaLI	GTGCAC	1 cut(s)	1557
ApeKI	GCWGC	6 cut(s)	46, 895, 947, 950, 1388, 1900
Asp700I	GAANNNNTTC	2 cut(s)	374, 730
Asp718I	GGTACC	1 cut(s)	1758
AspLEI	GCGC	4 cut(s)	92, 998, 1663, 1863
AspS9I	GGNCC	8 cut(s)	103, 311, 421, 1104, 1245, 1344, 1368, 1520
AsuC2I	CCSGG	4 cut(s)	219, 392, 869, 885
AsuHPI	GGTGA	1 cut(s)	475
AvaI	CYCGRG	2 cut(s)	244, 1485
AvaII	GGWCC	6 cut(s)	311, 421, 1104, 1344, 1368, 1520
AxyI	CCTNAGG	3 cut(s)	491, 1241, 1754
BaeGI	GKGCMC	2 cut(s)	1239, 1561
BamHI	GGATCC	1 cut(s)	764
BanI	GGYRCC	2 cut(s)	1234, 1758
BanII	GRGCYC	2 cut(s)	842, 1752
BauI	CACGAG	1 cut(s)	782
BbsI	GAAGAC	3 cut(s)	206, 839, 927
Bbv12I	GWGCWC	3 cut(s)	842, 1561, 1752
BbvCI	CCTCAGC	1 cut(s)	1157
BbvI	GCAGC	6 cut(s)	33, 882, 934, 937, 1375, 1912
BccI	CCATC	2 cut(s)	958, 1774
BceAI	ACGGC	4 cut(s)	101, 700, 983, 1130
BcgI	CGANNNNNNTGC	2 cut(s)	592, 626
BciT130I	CCWGG	1 cut(s)	678
BciVI	GTATCC	4 cut(s)	583, 914, 1597, 1833
BcnI	CCSGG	4 cut(s)	219, 392, 869, 885
BcoDI	GTCTC	5 cut(s)	278, 293, 299, 675, 1435
BcuI	ACTAGT	1 cut(s)	269
BfaI	CTAG	3 cut(s)	270, 1505, 1884
BfoI	RGCGCY	2 cut(s)	999, 1664
BfuI	GTATCC	4 cut(s)	583, 914, 1597, 1833
BglII	AGATCT	2 cut(s)	73, 459
Bme1390I	CCNGG	5 cut(s)	219, 392, 678, 869, 885
Bme18I	GGWCC	6 cut(s)	311, 421, 1104, 1344, 1368, 1520
BmeRI	GACNNNNNGTC	1 cut(s)	1930
BmeT110I	CYCGRG	2 cut(s)	244, 1485
BmgT120I	GGNCC	8 cut(s)	103, 311, 421, 1104, 1245, 1344, 1368, 1520
BmiI	GGNNCC	8 cut(s)	312, 371, 766, 1105, 1236, 1346, 1522, 1760
BmrFI	CCNGG	5 cut(s)	219, 392, 678, 869, 885
BmrI	ACTGGG	2 cut(s)	476, 1650
BmsI	GCATC	1 cut(s)	658
BmuI	ACTGGG	2 cut(s)	476, 1650
BpiI	GAAGAC	3 cut(s)	206, 839, 927
BplI	GAGNNNNNCTC	2 cut(s)	1671, 1703
BpmI	CTGGAG	4 cut(s)	546, 699, 1073, 1145
Bpu10I	CCTNAGC	2 cut(s)	537, 1157
BpuEI	CTTGAG	2 cut(s)	1401, 1641
BpuMI	CCSGG	4 cut(s)	219, 392, 869, 885
BsaHI	GRCGYC	2 cut(s)	177, 437
BsaJI	CCNNGG	7 cut(s)	676, 900, 1381, 1393, 1691, 1905, 1915
BsaXI	ACNNNNNCTCC	8 cut(s)	362, 386, 392, 416, 1046, 1076, 1169, 1199
Bsc4I	CCNNNNNNNGG	5 cut(s)	202, 492, 683, 1152, 1696
Bse118I	RCCGGY	1 cut(s)	749
Bse1I	ACTGG	4 cut(s)	105, 471, 562, 1645
Bse21I	CCTNAGG	3 cut(s)	491, 1241, 1754
Bse3DI	GCAATG	2 cut(s)	60, 1036
BseBI	CCWGG	1 cut(s)	678
BseDI	CCNNGG	7 cut(s)	676, 900, 1381, 1393, 1691, 1905, 1915
BseGI	GGATG	5 cut(s)	877, 1168, 1451, 1458, 1526
BseLI	CCNNNNNNNGG	5 cut(s)	202, 492, 683, 1152, 1696
BseMI	GCAATG	2 cut(s)	60, 1036
BseMII	CTCAG	6 cut(s)	298, 528, 1148, 1232, 1768, 1832
BseNI	ACTGG	4 cut(s)	105, 471, 562, 1645
BseRI	GAGGAG	7 cut(s)	28, 705, 1733, 1742, 1787, 1790, 1883
BseSI	GKGCMC	2 cut(s)	1239, 1561
BseX3I	CGGCCG	1 cut(s)	124
BseXI	GCAGC	6 cut(s)	33, 882, 934, 937, 1375, 1912
BseYI	CCCAGC	1 cut(s)	1130
BsgI	GTGCAG	1 cut(s)	1296
Bsh1236I	CGCG	3 cut(s)	90, 902, 1907
Bsh1285I	CGRYCG	1 cut(s)	127
BshFI	GGCC	7 cut(s)	104, 126, 1145, 1247, 1422, 1503, 1598
BshNI	GGYRCC	2 cut(s)	1234, 1758
BsiEI	CGRYCG	1 cut(s)	127
BsiHKAI	GWGCWC	3 cut(s)	842, 1561, 1752
BsiHKCI	CYCGRG	2 cut(s)	244, 1485
BsiSI	CCGG	9 cut(s)	219, 234, 381, 392, 424, 453, 750, 869, 885
BslFI	GGGAC	4 cut(s)	1108, 1117, 1147, 1506
BslI	CCNNNNNNNGG	5 cut(s)	202, 492, 683, 1152, 1696
BsmAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1435
BsmBI	CGTCTC	2 cut(s)	299, 675
BsmFI	GGGAC	4 cut(s)	1108, 1117, 1147, 1506
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	7 cut(s)	104, 126, 1145, 1247, 1422, 1503, 1598
BsoBI	CYCGRG	2 cut(s)	244, 1485
Bsp1286I	GDGCHC	4 cut(s)	842, 1239, 1561, 1752
Bsp1407I	TGTACA	1 cut(s)	402
Bsp143I	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
BspANI	GGCC	7 cut(s)	104, 126, 1145, 1247, 1422, 1503, 1598
BspCNI	CTCAG	6 cut(s)	297, 529, 1149, 1233, 1767, 1831
BspFNI	CGCG	3 cut(s)	90, 902, 1907
BspHI	TCATGA	1 cut(s)	1222
BspLI	GGNNCC	8 cut(s)	312, 371, 766, 1105, 1236, 1346, 1522, 1760
BspPI	GGATC	4 cut(s)	759, 772, 1227, 1568
BspQI	GCTCTTC	1 cut(s)	818
BspT107I	GGYRCC	2 cut(s)	1234, 1758
BsrBI	CCGCTC	1 cut(s)	95
BsrDI	GCAATG	2 cut(s)	60, 1036
BsrFI	RCCGGY	1 cut(s)	749
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	4 cut(s)	105, 471, 562, 1645
BssAI	RCCGGY	1 cut(s)	749
BssECI	CCNNGG	7 cut(s)	676, 900, 1381, 1393, 1691, 1905, 1915
BssMI	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
BssNI	GRCGYC	2 cut(s)	177, 437
BssSI	CACGAG	1 cut(s)	782
BssT1I	CCWWGG	2 cut(s)	1381, 1691
Bst2BI	CACGAG	1 cut(s)	782
Bst2UI	CCWGG	1 cut(s)	678
Bst4CI	ACNGT	7 cut(s)	185, 197, 407, 859, 1138, 1429, 1556
Bst6I	CTCTTC	4 cut(s)	351, 628, 818, 1145
BstACI	GRCGYC	2 cut(s)	177, 437
BstAUI	TGTACA	1 cut(s)	402
BstC8I	GCNNGC	7 cut(s)	175, 656, 673, 900, 1339, 1889, 1905
BstDEI	CTNAG	7 cut(s)	284, 491, 537, 1157, 1241, 1754, 1818
BstDSI	CCRYGG	4 cut(s)	900, 1393, 1905, 1915
BstF5I	GGATG	5 cut(s)	877, 1168, 1451, 1458, 1526
BstFNI	CGCG	3 cut(s)	90, 902, 1907
BstH2I	RGCGCY	2 cut(s)	999, 1664
BstHHI	GCGC	4 cut(s)	92, 998, 1663, 1863
BstKTI	GATC	8 cut(s)	76, 328, 462, 762, 767, 1051, 1222, 1576
BstMAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1435
BstMBI	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	1 cut(s)	678
BstNSI	RCATGY	1 cut(s)	1294
BstSCI	CCNGG	5 cut(s)	217, 390, 676, 867, 883
BstSLI	GKGCMC	2 cut(s)	1239, 1561
BstUI	CGCG	3 cut(s)	90, 902, 1907
BstV1I	GCAGC	6 cut(s)	33, 882, 934, 937, 1375, 1912
BstV2I	GAAGAC	3 cut(s)	206, 839, 927
BstX2I	RGATCY	3 cut(s)	73, 459, 764
BstXI	CCANNNNNNTGG	2 cut(s)	168, 1356
BstYI	RGATCY	3 cut(s)	73, 459, 764
BstZI	CGGCCG	1 cut(s)	124
Bsu36I	CCTNAGG	3 cut(s)	491, 1241, 1754
BsuI	GTATCC	4 cut(s)	583, 914, 1597, 1833
BsuRI	GGCC	7 cut(s)	104, 126, 1145, 1247, 1422, 1503, 1598
BtgI	CCRYGG	4 cut(s)	900, 1393, 1905, 1915
BtgZI	GCGATG	2 cut(s)	835, 977
BtsCI	GGATG	5 cut(s)	877, 1168, 1451, 1458, 1526
BtsIMutI	CAGTG	1 cut(s)	464
Cac8I	GCNNGC	7 cut(s)	175, 656, 673, 900, 1339, 1889, 1905
CciI	TCATGA	1 cut(s)	1222
CfoI	GCGC	4 cut(s)	92, 998, 1663, 1863
Cfr10I	RCCGGY	1 cut(s)	749
Cfr13I	GGNCC	8 cut(s)	103, 311, 421, 1104, 1245, 1344, 1368, 1520
Cfr42I	CCGCGG	2 cut(s)	903, 1908
CseI	GACGC	2 cut(s)	166, 426
Csp6I	GTAC	2 cut(s)	403, 1759
CviAII	CATG	6 cut(s)	162, 703, 793, 1223, 1291, 1436
CviQI	GTAC	2 cut(s)	403, 1759
DdeI	CTNAG	7 cut(s)	284, 491, 537, 1157, 1241, 1754, 1818
DpnI	GATC	8 cut(s)	75, 327, 461, 761, 766, 1050, 1221, 1575
DpnII	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
DraI	TTTAAA	1 cut(s)	1189
DrdI	GACNNNNNNGTC	1 cut(s)	197
DriI	GACNNNNNGTC	1 cut(s)	1930
DseDI	GACNNNNNNGTC	1 cut(s)	197
EaeI	YGGCCR	2 cut(s)	124, 1143
EagI	CGGCCG	1 cut(s)	124
Eam1104I	CTCTTC	4 cut(s)	351, 628, 818, 1145
Eam1105I	GACNNNNNGTC	1 cut(s)	1930
EarI	CTCTTC	4 cut(s)	351, 628, 818, 1145
EciI	GGCGGA	4 cut(s)	103, 322, 702, 1091
Ecl136II	GAGCTC	2 cut(s)	840, 1750
EclXI	CGGCCG	1 cut(s)	124
Eco130I	CCWWGG	2 cut(s)	1381, 1691
Eco24I	GRGCYC	2 cut(s)	842, 1752
Eco47I	GGWCC	6 cut(s)	311, 421, 1104, 1344, 1368, 1520
Eco47III	AGCGCT	1 cut(s)	1662
Eco52I	CGGCCG	1 cut(s)	124
Eco53kI	GAGCTC	2 cut(s)	840, 1750
Eco57I	CTGAAG	2 cut(s)	563, 1713
Eco81I	CCTNAGG	3 cut(s)	491, 1241, 1754
Eco88I	CYCGRG	2 cut(s)	244, 1485
EcoICRI	GAGCTC	2 cut(s)	840, 1750
EcoO109I	RGGNCCY	3 cut(s)	311, 1245, 1344
EcoRII	CCWGG	1 cut(s)	676
EcoT14I	CCWWGG	2 cut(s)	1381, 1691
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	842, 1752
ErhI	CCWWGG	2 cut(s)	1381, 1691
Esp3I	CGTCTC	2 cut(s)	299, 675
FaeI	CATG	6 cut(s)	165, 706, 796, 1226, 1294, 1439
FaqI	GGGAC	4 cut(s)	1108, 1117, 1147, 1506
FatI	CATG	6 cut(s)	161, 702, 792, 1222, 1290, 1435
FauI	CCCGC	4 cut(s)	494, 647, 907, 1912
FblI	GTMKAC	2 cut(s)	409, 846
FokI	GGATG	5 cut(s)	884, 1175, 1438, 1445, 1513
FriOI	GRGCYC	2 cut(s)	842, 1752
FspBI	CTAG	3 cut(s)	270, 1505, 1884
GlaI	GCGC	4 cut(s)	91, 997, 1662, 1862
GsaI	CCCAGC	1 cut(s)	1134
GsuI	CTGGAG	4 cut(s)	546, 699, 1073, 1145
HaeII	RGCGCY	2 cut(s)	999, 1664
HaeIII	GGCC	7 cut(s)	104, 126, 1145, 1247, 1422, 1503, 1598
HapII	CCGG	9 cut(s)	219, 234, 381, 392, 424, 453, 750, 869, 885
HgaI	GACGC	2 cut(s)	166, 426
HhaI	GCGC	4 cut(s)	92, 998, 1663, 1863
Hin1I	GRCGYC	2 cut(s)	177, 437
Hin1II	CATG	6 cut(s)	165, 706, 796, 1226, 1294, 1439
Hin6I	GCGC	4 cut(s)	90, 996, 1661, 1861
HinP1I	GCGC	4 cut(s)	90, 996, 1661, 1861
HincII	GTYRAC	3 cut(s)	181, 847, 1684
HindII	GTYRAC	3 cut(s)	181, 847, 1684
HindIII	AAGCTT	5 cut(s)	518, 545, 728, 1271, 1303
HinfI	GANTC	3 cut(s)	208, 532, 880
HpaII	CCGG	9 cut(s)	219, 234, 381, 392, 424, 453, 750, 869, 885
HphI	GGTGA	1 cut(s)	475
Hpy166II	GTNNAC	7 cut(s)	148, 181, 410, 847, 1559, 1684, 1725
Hpy188I	TCNGA	9 cut(s)	85, 267, 325, 603, 764, 1177, 1495, 1732, 1849
Hpy8I	GTNNAC	7 cut(s)	148, 181, 410, 847, 1559, 1684, 1725
Hpy99I	CGWCG	2 cut(s)	122, 848
HpyAV	CCTTC	5 cut(s)	330, 1084, 1210, 1670, 1848
HpyCH4III	ACNGT	7 cut(s)	185, 197, 407, 859, 1138, 1429, 1556
HpyCH4IV	ACGT	1 cut(s)	1876
HpyCH4V	TGCA	9 cut(s)	4, 65, 364, 613, 1313, 1321, 1559, 1795, 1802
HpyF3I	CTNAG	7 cut(s)	284, 491, 537, 1157, 1241, 1754, 1818
HpySE526I	ACGT	1 cut(s)	1876
Hsp92I	GRCGYC	2 cut(s)	177, 437
Hsp92II	CATG	6 cut(s)	165, 706, 796, 1226, 1294, 1439
HspAI	GCGC	4 cut(s)	90, 996, 1661, 1861
KpnI	GGTACC	1 cut(s)	1762
KspI	CCGCGG	2 cut(s)	903, 1908
Kzo9I	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
LguI	GCTCTTC	1 cut(s)	818
LmnI	GCTCC	8 cut(s)	60, 527, 845, 1334, 1385, 1630, 1755, 1870
Lsp1109I	GCAGC	6 cut(s)	33, 882, 934, 937, 1375, 1912
LweI	GCATC	1 cut(s)	658
MaeI	CTAG	3 cut(s)	270, 1505, 1884
MaeII	ACGT	1 cut(s)	1876
MaeIII	GTNAC	6 cut(s)	199, 340, 350, 1165, 1567, 1852
MalI	GATC	8 cut(s)	75, 327, 461, 761, 766, 1050, 1221, 1575
MbiI	CCGCTC	1 cut(s)	95
MboI	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
MboII	GAAGA	8 cut(s)	206, 338, 615, 832, 835, 844, 927, 1162
MflI	RGATCY	3 cut(s)	73, 459, 764
MhlI	GDGCHC	4 cut(s)	842, 1239, 1561, 1752
MluCI	AATT	7 cut(s)	445, 580, 643, 1170, 1354, 1374, 1398
MmeI	TCCRAC	3 cut(s)	465, 600, 1479
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	374, 730
MseI	TTAA	3 cut(s)	305, 567, 1188
MslI	CAYNNNNRTG	3 cut(s)	166, 870, 1295
MspA1I	CMGCKG	2 cut(s)	902, 1907
MspI	CCGG	9 cut(s)	219, 234, 381, 392, 424, 453, 750, 869, 885
MspR9I	CCNGG	5 cut(s)	219, 392, 678, 869, 885
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	1 cut(s)	678
MvnI	CGCG	3 cut(s)	90, 902, 1907
NciI	CCSGG	4 cut(s)	219, 392, 869, 885
NdeII	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
NlaIII	CATG	6 cut(s)	165, 706, 796, 1226, 1294, 1439
NlaIV	GGNNCC	8 cut(s)	312, 371, 766, 1105, 1236, 1346, 1522, 1760
NmuCI	GTSAC	3 cut(s)	199, 1165, 1852
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	1294
PagI	TCATGA	1 cut(s)	1222
PciSI	GCTCTTC	1 cut(s)	818
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	2 cut(s)	374, 730
PfeI	GAWTC	3 cut(s)	208, 532, 880
PflFI	GACNNNGTC	1 cut(s)	419
PfoI	TCCNGGA	1 cut(s)	390
PpuMI	RGGWCCY	2 cut(s)	311, 1344
Psp124BI	GAGCTC	2 cut(s)	842, 1752
Psp5II	RGGWCCY	2 cut(s)	311, 1344
Psp6I	CCWGG	1 cut(s)	676
PspFI	CCCAGC	1 cut(s)	1130
PspGI	CCWGG	1 cut(s)	676
PspN4I	GGNNCC	8 cut(s)	312, 371, 766, 1105, 1236, 1346, 1522, 1760
PspPI	GGNCC	8 cut(s)	103, 311, 421, 1104, 1245, 1344, 1368, 1520
PspPPI	RGGWCCY	2 cut(s)	311, 1344
PsuI	RGATCY	3 cut(s)	73, 459, 764
PsyI	GACNNNGTC	1 cut(s)	419
RsaI	GTAC	2 cut(s)	404, 1760
RsaNI	GTAC	2 cut(s)	403, 1759
RseI	CAYNNNNRTG	3 cut(s)	166, 870, 1295
SacI	GAGCTC	2 cut(s)	842, 1752
SacII	CCGCGG	2 cut(s)	903, 1908
SalI	GTCGAC	1 cut(s)	845
SapI	GCTCTTC	1 cut(s)	818
SaqAI	TTAA	3 cut(s)	305, 567, 1188
Sau3AI	GATC	8 cut(s)	73, 325, 459, 759, 764, 1048, 1219, 1573
Sau96I	GGNCC	8 cut(s)	103, 311, 421, 1104, 1245, 1344, 1368, 1520
ScrFI	CCNGG	5 cut(s)	219, 392, 678, 869, 885
SduI	GDGCHC	4 cut(s)	842, 1239, 1561, 1752
SfaNI	GCATC	1 cut(s)	658
Sfr303I	CCGCGG	2 cut(s)	903, 1908
SgrBI	CCGCGG	2 cut(s)	903, 1908
SinI	GGWCC	6 cut(s)	311, 421, 1104, 1344, 1368, 1520
SmiMI	CAYNNNNRTG	3 cut(s)	166, 870, 1295
SmlI	CTYRAG	2 cut(s)	1416, 1656
SmoI	CTYRAG	2 cut(s)	1416, 1656
SpeI	ACTAGT	1 cut(s)	269
Sse9I	AATT	7 cut(s)	445, 580, 643, 1170, 1354, 1374, 1398
SspMI	CTAG	3 cut(s)	270, 1505, 1884
SstI	GAGCTC	2 cut(s)	842, 1752
StyD4I	CCNGG	5 cut(s)	217, 390, 676, 867, 883
StyI	CCWWGG	2 cut(s)	1381, 1691
TaaI	ACNGT	7 cut(s)	185, 197, 407, 859, 1138, 1429, 1556
TaiI	ACGT	1 cut(s)	1879
TaqI	TCGA	6 cut(s)	558, 846, 876, 1740, 1770, 1939
TasI	AATT	7 cut(s)	445, 580, 643, 1170, 1354, 1374, 1398
TatI	WGTACW	1 cut(s)	402
TauI	GCSGC	6 cut(s)	95, 126, 947, 956, 959, 962
TfiI	GAWTC	3 cut(s)	208, 532, 880
Tru1I	TTAA	3 cut(s)	305, 567, 1188
Tru9I	TTAA	3 cut(s)	305, 567, 1188
TscAI	CASTG	1 cut(s)	471
TseFI	GTSAC	3 cut(s)	199, 1165, 1852
TseI	GCWGC	6 cut(s)	46, 895, 947, 950, 1388, 1900
Tsp45I	GTSAC	3 cut(s)	199, 1165, 1852
TspDTI	ATGAA	3 cut(s)	719, 1239, 1494
TspGWI	ACGGA	6 cut(s)	281, 757, 832, 939, 1338, 1904
TspRI	CASTG	1 cut(s)	471
Tth111I	GACNNNGTC	1 cut(s)	419
VneI	GTGCAC	1 cut(s)	1557
VpaK11BI	GGWCC	6 cut(s)	311, 421, 1104, 1344, 1368, 1520
XceI	RCATGY	1 cut(s)	1294
XmiI	GTMKAC	2 cut(s)	409, 846
XmnI	GAANNNNTTC	2 cut(s)	374, 730
XspI	CTAG	3 cut(s)	270, 1505, 1884
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.