Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002683

Physical Location & Seq

Genomic Coordinates Reverse (-)

31750138 .. 31753274

Gene Structure 3137 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1238300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGCATTCCCAAACCCAAAGGCTTTGCCTTTTCCTCTTCTCCCTGCTGCTTTTGCTCCCCATTGCAAAACCAGATCTGGCTTCTGACCGCTCCGCTCTACTGGCCCTGCGCTCCGCCGTCGGCGGCCGAACTCTGCTCTGGAATGTGAACCAGACCAGCCCATGTTCGTGGGCTGGCGTCAACTGTGAGAATAACCGTGTCACGGGGATTCGTCTTCCCGGCGTGGCTCTATCCGGCGTAATTCCCAGTGGCATTTTTGGAAATCTGACCAGTCTCCGCACTCTCAGTCTCCGTCTCAACGCTTTAAGAGGTCCTCTGCCGTCAGATCTCTCCGCCTGTGTTACTCTTCGTAACCTTTACTTGCAGGGGAACCTTTTCTCCGGCGAAATCCCGGAGTTTTTGTACAGTCTACACGACTTGGTCAGGCTGAACTTGGCGTCCAACAATTTTTCCGGTGAGATCTCACTGGGTTTCAACAATCTCACCCGCCTGAGGACTTTGTATCTTGAAAGCAACAAGCTTTCTGGAGCGATTCCAGAGCTGAAGCTTCCTAATCTCGACCAGTTCAATGTATCCAGCAATTTGCTAAACGGGTCTGTTCCGAAGCAGTTGCAGTCTTACCCTTCAAGCTCGTTTCTGGGTAATTTGCTATGCGGGGGTCCGCTTGATGCTTGCCCTGGAGACGGCGGAGCTGCCGGAGGAGGAGATATAAACATCAATGACAACCACAAAAGGAGAAAGCTTTCAGGTGGTGCTATTGCCGGTATTGTGATCGGATCCGTGCTGGCTTTTCTCGTGATTATCATGCTTTTGATCTTTTTCTGCCGAAAGAAGAAGAGCAAGAAGACGAGCTCCGTTGACATCGCCACCGTGAAGCACCCGGAAGTCGAGATTCCCGGCGAGAAGCTGCCCGCGGAGGCTGAAAATGTGGGATACGGAAATGGGTCTTCAGTGGCGGCTGCTGCGGCGGCGGCGATGGTGGGGAATGGGAAAAGTGAAGCCAATAGCGCCGGTGGTGCTAAAAAGCTGGTGTTTTTTGGCAATGGGGCGAGGGTGTTTGATCTGGAGGACTTGTTGAGAGCTTCGGCGGAGGTTTTGGGGAAGGGGACTTTCGGGACCGCATACAAGGCGGTTCTGGAGGCTGGGACTGTGGTGGCTGTGAAGAGGCTGAGGGATGTGACAATTTCGGAGAGCGAGTTTAAAGAAAAGATTGAAGCTGTGGGAGTGAAGGATCATGAAAATTTGGTGCCTCTGAGGGCCTACTATTTCAGCAGAGATGAGAAGCTTCTTGTCTATGATTACATGCCTATGGGAAGCTTATCTGCACTTTTGCACGGAAACAAGGGAGCAGGCAGGACCCCATTGAATTGGGAAATCAGGTCCGGAATTGCCCTTGGAGCTGCCCGTGGAATTGAATACCTACACTCTCAAGGCCAAACTGTCTCGCATGGAAACATCAAGTCATCCAACATCCTGCTAACAAAGTCCTATGAAGCTCGGGTTTCTGACTTCGGCCTAGCTCATCTTGTTGGTCCCTCATCTACTCCCAACCGAGTTTCTGGCTACCGGGCACCAGAGGTTACTGATCCTCGCAAGGTATCCCAAAAGGCCGATGTTTATAGCTTTGGGGTATTGCTCTTGGAACTACTTACTGGGAAGCCCCCAACTCATGCCCTCTTGAATGAGGAAGGAGTTGACCTCCCAAGGTGGGTTCAGTCCATAGTCAAAGAGGAGTGGACTTCAGAGGTTTTCGATGTTGAGCTCCTCAGGTACCAGAATGTCGAGGAGGAGATGGTTCAGCTCTTGCAACTTGCAATAGATTGTTCGGCTCAGTATCCTGACAAACGCCCTTCAATCTCCGAAGTGACAAGGCGCATTGAGGAGCTACGTCGTTCTAGCTTGCGAGATGAGCAGCCCGAGGTTGTCCGTGGTTTAGACAACGTATCCTCTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

652

Amino Acids

70.36

Weight (kDa)

8.59

Isoelectric Point (pI)

41.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	27 - 62	9.7e-09	Leucine rich repeat N-terminal domain
Pkinase	PF00069	364 - 623	5.9e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	364 - 625	1.8e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48480
fragaria_vesca	FvH4_2g12190 FvH4_2g12190 FvH4_5g17570
malus_domestica	MD02G1259100.v1.1 MD06G1231600.v1.1 MD09G1086600.v1.1 MD09G1271200.v1.1 MD14G1238300.v1.1 MD17G1269300.v1.1
prunus_persica	Prupe.3G017300_v2.0.a1 Prupe.5G235500_v2.0.a1
pyrus_communis	pycom06g20550 pycom09g18220 pycom14g19950 pycom17g26800
rosa_chinensis	RchiOBHm_Chr3g0491091 RchiOBHm_Chr7g0178081
rosa_laevigata	RLG00000005473 RLG00000022865
rosa_multiflora	Rmu_co8170494.1_g000001 Rmu_co8379533.1_g000001 Rmu_co8445967.1_g000001 Rmu_sc0000425.1_g000007 Rmu_sc0005038.1_g000014 Rmu_ssc0000386.1_g000061 Rmu_ssc0000386.1_g000063
rosa_roxburghii	Rroxscaffold_6G00393220
rosa_rugosa	Rorug03G0246000 Rorug06G0413300
rosa_samantha	Rh3AG296200 Rh3BG332200 Rh3CG329300 Rh3DG329900 Rh7AG012600 Rh7BG012500 Rh7CG012500 Rh7DG012800
rosa_wichuraiana	Rw3G026140 Rw7G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1770
AccB1I	GGYRCC	3 cut(s)	1246, 1570, 1770
AccBSI	CCGCTC	2 cut(s)	90, 95
AccI	GTMKAC	1 cut(s)	409
AccII	CGCG	1 cut(s)	914
AccIII	TCCGGA	1 cut(s)	1382
AclWI	GGATC	4 cut(s)	771, 784, 1239, 1580
AcoI	YGGCCR	1 cut(s)	124
AcsI	RAATTY	1 cut(s)	1240
AcuI	CTGAAG	3 cut(s)	563, 933, 1725
AcyI	GRCGYC	2 cut(s)	177, 437
AfaI	GTAC	2 cut(s)	404, 1772
AfiI	CCNNNNNNNGG	4 cut(s)	202, 492, 683, 1708
AgsI	TTSAA	9 cut(s)	475, 509, 568, 627, 1214, 1366, 1415, 1681, 1854
AjnI	CCWGG	1 cut(s)	676
AloI	GAACNNNNNNTCC	4 cut(s)	362, 394, 422, 454
Alw21I	GWGCWC	2 cut(s)	854, 1764
Alw26I	GTCTC	5 cut(s)	278, 293, 299, 675, 1447
AlwI	GGATC	4 cut(s)	771, 784, 1239, 1580
Ama87I	CYCGRG	2 cut(s)	1497, 1916
Aor13HI	TCCGGA	1 cut(s)	1382
AoxI	GGCC	6 cut(s)	102, 124, 1257, 1432, 1513, 1608
ApeKI	GCWGC	7 cut(s)	46, 692, 907, 959, 962, 1400, 1912
ApoI	RAATTY	1 cut(s)	1240
Asp700I	GAANNNNTTC	2 cut(s)	374, 742
Asp718I	GGTACC	1 cut(s)	1770
AspLEI	GCGC	3 cut(s)	111, 1010, 1875
AspS9I	GGNCC	8 cut(s)	103, 311, 659, 1116, 1257, 1356, 1380, 1532
AsuC2I	CCSGG	5 cut(s)	219, 392, 881, 897, 1568
AsuHPI	GGTGA	2 cut(s)	467, 475
AvaI	CYCGRG	2 cut(s)	1497, 1916
AvaII	GGWCC	6 cut(s)	311, 659, 1116, 1356, 1380, 1532
AxyI	CCTNAGG	2 cut(s)	491, 1766
BaeGI	GKGCMC	1 cut(s)	1573
BamHI	GGATCC	1 cut(s)	776
BanI	GGYRCC	3 cut(s)	1246, 1570, 1770
BanII	GRGCYC	2 cut(s)	854, 1764
BauI	CACGAG	1 cut(s)	794
BbsI	GAAGAC	3 cut(s)	206, 851, 939
Bbv12I	GWGCWC	2 cut(s)	854, 1764
BbvCI	CCTCAGC	1 cut(s)	1169
BbvI	GCAGC	7 cut(s)	33, 679, 894, 946, 949, 1387, 1924
BccI	CCATC	2 cut(s)	970, 1786
BceAI	ACGGC	3 cut(s)	101, 304, 700
BcgI	CGANNNNNNTGC	2 cut(s)	592, 626
BciT130I	CCWGG	1 cut(s)	678
BciVI	GTATCC	5 cut(s)	583, 926, 1609, 1845, 1954
BcnI	CCSGG	5 cut(s)	219, 392, 881, 897, 1568
BcoDI	GTCTC	5 cut(s)	278, 293, 299, 675, 1447
BfaI	CTAG	2 cut(s)	1517, 1896
BfoI	RGCGCY	1 cut(s)	1011
BfuI	GTATCC	5 cut(s)	583, 926, 1609, 1845, 1954
BglII	AGATCT	3 cut(s)	73, 325, 459
Bme1390I	CCNGG	6 cut(s)	219, 392, 678, 881, 897, 1568
Bme18I	GGWCC	6 cut(s)	311, 659, 1116, 1356, 1380, 1532
BmeT110I	CYCGRG	2 cut(s)	1497, 1916
BmgT120I	GGNCC	8 cut(s)	103, 311, 659, 1116, 1257, 1356, 1380, 1532
BmiI	GGNNCC	9 cut(s)	371, 660, 778, 1117, 1248, 1358, 1534, 1572, 1772
BmrFI	CCNGG	6 cut(s)	219, 392, 678, 881, 897, 1568
BmrI	ACTGGG	3 cut(s)	240, 476, 1662
BmsI	GCATC	1 cut(s)	658
BmuI	ACTGGG	3 cut(s)	240, 476, 1662
BpiI	GAAGAC	3 cut(s)	206, 851, 939
BplI	GAGNNNNNCTC	2 cut(s)	1683, 1715
BpmI	CTGGAG	4 cut(s)	546, 699, 1085, 1157
Bpu10I	CCTNAGC	1 cut(s)	1169
BpuEI	CTTGAG	1 cut(s)	1413
BpuMI	CCSGG	5 cut(s)	219, 392, 881, 897, 1568
BsaHI	GRCGYC	2 cut(s)	177, 437
BsaJI	CCNNGG	7 cut(s)	676, 912, 1393, 1405, 1703, 1917, 1927
BsaWI	WCCGGW	2 cut(s)	452, 1382
BsaXI	ACNNNNNCTCC	8 cut(s)	362, 386, 392, 416, 1058, 1088, 1181, 1211
Bsc4I	CCNNNNNNNGG	4 cut(s)	202, 492, 683, 1708
Bse118I	RCCGGY	2 cut(s)	761, 1010
Bse1I	ACTGG	6 cut(s)	105, 246, 270, 471, 562, 1657
Bse21I	CCTNAGG	2 cut(s)	491, 1766
Bse3DI	GCAATG	2 cut(s)	60, 1048
BseAI	TCCGGA	1 cut(s)	1382
BseBI	CCWGG	1 cut(s)	678
BseDI	CCNNGG	7 cut(s)	676, 912, 1393, 1405, 1703, 1917, 1927
BseGI	GGATG	3 cut(s)	1180, 1463, 1470
BseLI	CCNNNNNNNGG	4 cut(s)	202, 492, 683, 1708
BseMI	GCAATG	2 cut(s)	60, 1048
BseMII	CTCAG	6 cut(s)	298, 482, 1160, 1244, 1780, 1844
BseNI	ACTGG	6 cut(s)	105, 246, 270, 471, 562, 1657
BseRI	GAGGAG	7 cut(s)	714, 717, 1745, 1754, 1799, 1802, 1895
BseSI	GKGCMC	1 cut(s)	1573
BseX3I	CGGCCG	1 cut(s)	124
BseXI	GCAGC	7 cut(s)	33, 679, 894, 946, 949, 1387, 1924
BseYI	CCCAGC	1 cut(s)	1142
BsgI	GTGCAG	1 cut(s)	1308
Bsh1236I	CGCG	1 cut(s)	914
Bsh1285I	CGRYCG	1 cut(s)	127
BshFI	GGCC	6 cut(s)	104, 126, 1259, 1434, 1515, 1610
BshNI	GGYRCC	3 cut(s)	1246, 1570, 1770
BsiEI	CGRYCG	1 cut(s)	127
BsiHKAI	GWGCWC	2 cut(s)	854, 1764
BsiHKCI	CYCGRG	2 cut(s)	1497, 1916
BslFI	GGGAC	4 cut(s)	1120, 1129, 1159, 1518
BslI	CCNNNNNNNGG	4 cut(s)	202, 492, 683, 1708
BsmAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1447
BsmBI	CGTCTC	2 cut(s)	299, 675
BsmFI	GGGAC	4 cut(s)	1120, 1129, 1159, 1518
BsmI	GAATGC	1 cut(s)	4
BsnI	GGCC	6 cut(s)	104, 126, 1259, 1434, 1515, 1610
BsoBI	CYCGRG	2 cut(s)	1497, 1916
Bsp1286I	GDGCHC	3 cut(s)	854, 1573, 1764
Bsp13I	TCCGGA	1 cut(s)	1382
Bsp1407I	TGTACA	1 cut(s)	402
Bsp143I	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
BspANI	GGCC	6 cut(s)	104, 126, 1259, 1434, 1515, 1610
BspCNI	CTCAG	6 cut(s)	297, 483, 1161, 1245, 1779, 1843
BspEI	TCCGGA	1 cut(s)	1382
BspFNI	CGCG	1 cut(s)	914
BspHI	TCATGA	1 cut(s)	1234
BspLI	GGNNCC	9 cut(s)	371, 660, 778, 1117, 1248, 1358, 1534, 1572, 1772
BspPI	GGATC	4 cut(s)	771, 784, 1239, 1580
BspQI	GCTCTTC	1 cut(s)	830
BspT107I	GGYRCC	3 cut(s)	1246, 1570, 1770
BsrBI	CCGCTC	2 cut(s)	90, 95
BsrDI	GCAATG	2 cut(s)	60, 1048
BsrFI	RCCGGY	2 cut(s)	761, 1010
BsrGI	TGTACA	1 cut(s)	402
BsrI	ACTGG	6 cut(s)	105, 246, 270, 471, 562, 1657
BssAI	RCCGGY	2 cut(s)	761, 1010
BssECI	CCNNGG	7 cut(s)	676, 912, 1393, 1405, 1703, 1917, 1927
BssMI	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
BssNI	GRCGYC	2 cut(s)	177, 437
BssSI	CACGAG	1 cut(s)	794
BssT1I	CCWWGG	2 cut(s)	1393, 1703
Bst2BI	CACGAG	1 cut(s)	794
Bst2UI	CCWGG	1 cut(s)	678
Bst4CI	ACNGT	6 cut(s)	185, 197, 407, 871, 1150, 1441
Bst6I	CTCTTC	4 cut(s)	41, 351, 830, 1157
BstACI	GRCGYC	2 cut(s)	177, 437
BstAUI	TGTACA	1 cut(s)	402
BstC8I	GCNNGC	6 cut(s)	175, 673, 786, 912, 1351, 1901
BstDEI	CTNAG	6 cut(s)	284, 491, 1169, 1253, 1766, 1830
BstDSI	CCRYGG	3 cut(s)	912, 1405, 1927
BstF5I	GGATG	3 cut(s)	1180, 1463, 1470
BstFNI	CGCG	1 cut(s)	914
BstH2I	RGCGCY	1 cut(s)	1011
BstHHI	GCGC	3 cut(s)	111, 1010, 1875
BstKTI	GATC	9 cut(s)	76, 328, 462, 774, 779, 816, 1063, 1234, 1588
BstMAI	GTCTC	5 cut(s)	278, 293, 299, 675, 1447
BstMBI	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	1 cut(s)	678
BstNSI	RCATGY	1 cut(s)	1306
BstSCI	CCNGG	6 cut(s)	217, 390, 676, 879, 895, 1566
BstSLI	GKGCMC	1 cut(s)	1573
BstUI	CGCG	1 cut(s)	914
BstV1I	GCAGC	7 cut(s)	33, 679, 894, 946, 949, 1387, 1924
BstV2I	GAAGAC	3 cut(s)	206, 851, 939
BstX2I	RGATCY	4 cut(s)	73, 325, 459, 776
BstXI	CCANNNNNNTGG	2 cut(s)	168, 1368
BstYI	RGATCY	4 cut(s)	73, 325, 459, 776
BstZI	CGGCCG	1 cut(s)	124
Bsu36I	CCTNAGG	2 cut(s)	491, 1766
BsuI	GTATCC	5 cut(s)	583, 926, 1609, 1845, 1954
BsuRI	GGCC	6 cut(s)	104, 126, 1259, 1434, 1515, 1610
BtgI	CCRYGG	3 cut(s)	912, 1405, 1927
BtgZI	GCGATG	2 cut(s)	847, 989
BtsCI	GGATG	3 cut(s)	1180, 1463, 1470
BtsIMutI	CAGTG	3 cut(s)	253, 464, 957
Cac8I	GCNNGC	6 cut(s)	175, 673, 786, 912, 1351, 1901
CciI	TCATGA	1 cut(s)	1234
CfoI	GCGC	3 cut(s)	111, 1010, 1875
Cfr10I	RCCGGY	2 cut(s)	761, 1010
Cfr13I	GGNCC	8 cut(s)	103, 311, 659, 1116, 1257, 1356, 1380, 1532
Cfr42I	CCGCGG	1 cut(s)	915
CseI	GACGC	2 cut(s)	166, 426
Csp6I	GTAC	2 cut(s)	403, 1771
CviAII	CATG	6 cut(s)	162, 805, 1235, 1303, 1448, 1670
CviQI	GTAC	2 cut(s)	403, 1771
DdeI	CTNAG	6 cut(s)	284, 491, 1169, 1253, 1766, 1830
DpnI	GATC	9 cut(s)	75, 327, 461, 773, 778, 815, 1062, 1233, 1587
DpnII	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
DraI	TTTAAA	1 cut(s)	1201
EaeI	YGGCCR	1 cut(s)	124
EagI	CGGCCG	1 cut(s)	124
Eam1104I	CTCTTC	4 cut(s)	41, 351, 830, 1157
EarI	CTCTTC	4 cut(s)	41, 351, 830, 1157
EciI	GGCGGA	4 cut(s)	103, 322, 702, 1103
Ecl136II	GAGCTC	2 cut(s)	852, 1762
EclXI	CGGCCG	1 cut(s)	124
Eco130I	CCWWGG	2 cut(s)	1393, 1703
Eco24I	GRGCYC	2 cut(s)	854, 1764
Eco47I	GGWCC	6 cut(s)	311, 659, 1116, 1356, 1380, 1532
Eco52I	CGGCCG	1 cut(s)	124
Eco53kI	GAGCTC	2 cut(s)	852, 1762
Eco57I	CTGAAG	3 cut(s)	563, 933, 1725
Eco81I	CCTNAGG	2 cut(s)	491, 1766
Eco88I	CYCGRG	2 cut(s)	1497, 1916
EcoICRI	GAGCTC	2 cut(s)	852, 1762
EcoO109I	RGGNCCY	3 cut(s)	311, 1257, 1356
EcoRII	CCWGG	1 cut(s)	676
EcoT14I	CCWWGG	2 cut(s)	1393, 1703
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	854, 1764
ErhI	CCWWGG	2 cut(s)	1393, 1703
Esp3I	CGTCTC	2 cut(s)	299, 675
FaeI	CATG	6 cut(s)	165, 808, 1238, 1306, 1451, 1673
FaqI	GGGAC	4 cut(s)	1120, 1129, 1159, 1518
FatI	CATG	6 cut(s)	161, 804, 1234, 1302, 1447, 1669
FauI	CCCGC	3 cut(s)	494, 647, 919
FblI	GTMKAC	1 cut(s)	409
FokI	GGATG	3 cut(s)	1187, 1450, 1457
FriOI	GRGCYC	2 cut(s)	854, 1764
FspBI	CTAG	2 cut(s)	1517, 1896
GlaI	GCGC	3 cut(s)	110, 1009, 1874
GsaI	CCCAGC	1 cut(s)	1146
GsuI	CTGGAG	4 cut(s)	546, 699, 1085, 1157
HaeII	RGCGCY	1 cut(s)	1011
HaeIII	GGCC	6 cut(s)	104, 126, 1259, 1434, 1515, 1610
HgaI	GACGC	2 cut(s)	166, 426
HhaI	GCGC	3 cut(s)	111, 1010, 1875
Hin1I	GRCGYC	2 cut(s)	177, 437
Hin1II	CATG	6 cut(s)	165, 808, 1238, 1306, 1451, 1673
Hin6I	GCGC	3 cut(s)	109, 1008, 1873
HinP1I	GCGC	3 cut(s)	109, 1008, 1873
HincII	GTYRAC	3 cut(s)	181, 859, 1696
HindII	GTYRAC	3 cut(s)	181, 859, 1696
HindIII	AAGCTT	5 cut(s)	518, 545, 740, 1283, 1315
HinfI	GANTC	3 cut(s)	208, 532, 892
HphI	GGTGA	2 cut(s)	467, 475
Hpy166II	GTNNAC	6 cut(s)	148, 181, 410, 859, 1696, 1737
Hpy8I	GTNNAC	6 cut(s)	148, 181, 410, 859, 1696, 1737
Hpy99I	CGWCG	2 cut(s)	122, 1893
HpyAV	CCTTC	5 cut(s)	633, 1096, 1222, 1682, 1860
HpyCH4III	ACNGT	6 cut(s)	185, 197, 407, 871, 1150, 1441
HpyCH4IV	ACGT	2 cut(s)	1888, 1941
HpyCH4V	TGCA	8 cut(s)	4, 65, 364, 613, 1325, 1333, 1807, 1814
HpyF3I	CTNAG	6 cut(s)	284, 491, 1169, 1253, 1766, 1830
HpySE526I	ACGT	2 cut(s)	1888, 1941
Hsp92I	GRCGYC	2 cut(s)	177, 437
Hsp92II	CATG	6 cut(s)	165, 808, 1238, 1306, 1451, 1673
HspAI	GCGC	3 cut(s)	109, 1008, 1873
Kpn2I	TCCGGA	1 cut(s)	1382
KpnI	GGTACC	1 cut(s)	1774
KspI	CCGCGG	1 cut(s)	915
Kzo9I	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
LguI	GCTCTTC	1 cut(s)	830
Lsp1109I	GCAGC	7 cut(s)	33, 679, 894, 946, 949, 1387, 1924
LweI	GCATC	1 cut(s)	658
MaeI	CTAG	2 cut(s)	1517, 1896
MaeII	ACGT	2 cut(s)	1888, 1941
MaeIII	GTNAC	6 cut(s)	199, 340, 350, 1177, 1579, 1864
MalI	GATC	9 cut(s)	75, 327, 461, 773, 778, 815, 1062, 1233, 1587
MbiI	CCGCTC	2 cut(s)	90, 95
MboI	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
MboII	GAAGA	8 cut(s)	28, 206, 338, 844, 847, 856, 939, 1174
MflI	RGATCY	4 cut(s)	73, 325, 459, 776
MhlI	GDGCHC	3 cut(s)	854, 1573, 1764
MluCI	AATT	9 cut(s)	240, 445, 580, 643, 1182, 1240, 1366, 1386, 1410
MmeI	TCCRAC	2 cut(s)	465, 1491
Mph1103I	ATGCAT	1 cut(s)	6
MroI	TCCGGA	1 cut(s)	1382
MroXI	GAANNNNTTC	2 cut(s)	374, 742
MseI	TTAA	2 cut(s)	305, 1200
MslI	CAYNNNNRTG	2 cut(s)	166, 1307
MspA1I	CMGCKG	1 cut(s)	914
MspR9I	CCNGG	6 cut(s)	219, 392, 678, 881, 897, 1568
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	1 cut(s)	678
MvnI	CGCG	1 cut(s)	914
NciI	CCSGG	5 cut(s)	219, 392, 881, 897, 1568
NdeII	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
NlaIII	CATG	6 cut(s)	165, 808, 1238, 1306, 1451, 1673
NlaIV	GGNNCC	9 cut(s)	371, 660, 778, 1117, 1248, 1358, 1534, 1572, 1772
NmuCI	GTSAC	3 cut(s)	199, 1177, 1864
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	1306
PagI	TCATGA	1 cut(s)	1234
PciSI	GCTCTTC	1 cut(s)	830
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	2 cut(s)	374, 742
PfeI	GAWTC	3 cut(s)	208, 532, 892
PflFI	GACNNNGTC	1 cut(s)	419
PfoI	TCCNGGA	1 cut(s)	390
PpuMI	RGGWCCY	2 cut(s)	311, 1356
Psp124BI	GAGCTC	2 cut(s)	854, 1764
Psp5II	RGGWCCY	2 cut(s)	311, 1356
Psp6I	CCWGG	1 cut(s)	676
PspFI	CCCAGC	1 cut(s)	1142
PspGI	CCWGG	1 cut(s)	676
PspN4I	GGNNCC	9 cut(s)	371, 660, 778, 1117, 1248, 1358, 1534, 1572, 1772
PspPI	GGNCC	8 cut(s)	103, 311, 659, 1116, 1257, 1356, 1380, 1532
PspPPI	RGGWCCY	2 cut(s)	311, 1356
PsuI	RGATCY	4 cut(s)	73, 325, 459, 776
PsyI	GACNNNGTC	1 cut(s)	419
RsaI	GTAC	2 cut(s)	404, 1772
RsaNI	GTAC	2 cut(s)	403, 1771
RseI	CAYNNNNRTG	2 cut(s)	166, 1307
SacI	GAGCTC	2 cut(s)	854, 1764
SacII	CCGCGG	1 cut(s)	915
SapI	GCTCTTC	1 cut(s)	830
SaqAI	TTAA	2 cut(s)	305, 1200
Sau3AI	GATC	9 cut(s)	73, 325, 459, 771, 776, 813, 1060, 1231, 1585
Sau96I	GGNCC	8 cut(s)	103, 311, 659, 1116, 1257, 1356, 1380, 1532
ScrFI	CCNGG	6 cut(s)	219, 392, 678, 881, 897, 1568
SduI	GDGCHC	3 cut(s)	854, 1573, 1764
SfaNI	GCATC	1 cut(s)	658
Sfr303I	CCGCGG	1 cut(s)	915
SgrAI	CRCCGGYG	1 cut(s)	1010
SgrBI	CCGCGG	1 cut(s)	915
SinI	GGWCC	6 cut(s)	311, 659, 1116, 1356, 1380, 1532
SmiMI	CAYNNNNRTG	2 cut(s)	166, 1307
SmlI	CTYRAG	1 cut(s)	1428
SmoI	CTYRAG	1 cut(s)	1428
Sse9I	AATT	9 cut(s)	240, 445, 580, 643, 1182, 1240, 1366, 1386, 1410
SspMI	CTAG	2 cut(s)	1517, 1896
SstI	GAGCTC	2 cut(s)	854, 1764
StyD4I	CCNGG	6 cut(s)	217, 390, 676, 879, 895, 1566
StyI	CCWWGG	2 cut(s)	1393, 1703
TaaI	ACNGT	6 cut(s)	185, 197, 407, 871, 1150, 1441
TaiI	ACGT	2 cut(s)	1891, 1944
TaqI	TCGA	5 cut(s)	558, 888, 1752, 1782, 1951
TasI	AATT	9 cut(s)	240, 445, 580, 643, 1182, 1240, 1366, 1386, 1410
TatI	WGTACW	1 cut(s)	402
TauI	GCSGC	5 cut(s)	126, 959, 968, 971, 974
TfiI	GAWTC	3 cut(s)	208, 532, 892
Tru1I	TTAA	2 cut(s)	305, 1200
Tru9I	TTAA	2 cut(s)	305, 1200
TscAI	CASTG	3 cut(s)	253, 471, 957
TseFI	GTSAC	3 cut(s)	199, 1177, 1864
TseI	GCWGC	7 cut(s)	46, 692, 907, 959, 962, 1400, 1912
Tsp45I	GTSAC	3 cut(s)	199, 1177, 1864
TspDTI	ATGAA	2 cut(s)	1251, 1506
TspGWI	ACGGA	6 cut(s)	281, 769, 844, 951, 1350, 1916
TspRI	CASTG	3 cut(s)	253, 471, 957
Tth111I	GACNNNGTC	1 cut(s)	419
VpaK11BI	GGWCC	6 cut(s)	311, 659, 1116, 1356, 1380, 1532
XapI	RAATTY	1 cut(s)	1240
XceI	RCATGY	1 cut(s)	1306
XmiI	GTMKAC	1 cut(s)	409
XmnI	GAANNNNTTC	2 cut(s)	374, 742
XspI	CTAG	2 cut(s)	1517, 1896
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.