Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002683

Physical Location & Seq

Genomic Coordinates Reverse (-)

67933560 .. 67936124

Gene Structure 2565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005473

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGCATTCCCATTATCAAAGGCAGAGTCTTTTTTACCTCTGCTTTTTCTCCATACTAGTTTTGCTTCCATTAGCAACACCAGATCTGAGCTCCGACCGCGCCGCCCTTCTCGCTCTCCGCTCCGCCGTCGGAGGCCGGACCCTCCTCTGGGACGTGACCCGGCCCAGCCCATGTTCCTGGGCCGGAGTCAACTGCGAGGACAACCGCGTCACTGTGCTTCGTCTCCCTGGTGTGGCTCTCCACGGCACCATTCCCACTGGCATTTTCGGAAACTTGACCGGCCTTCGCACTCTCAGTCTTCGTCTCAATGCTCTCACCGGTCCTCTCCCGTCAGATCTCTCCGCCTGCGTTACTCTTCGTAACCTCTACTTACAGGGGAACTTGTTCTCCGGCGAAATCCCCGAGTTTCTGTACAGCTTGCATGACTTGGTCCGGCTCAACTTGGCCTCCAACAACTTCTCCGGCGAGATCTCACCTTCTTTCAACAATCTGACAAGGATAAGGACACTGTACCTCGAAAACAACAAGCTTTCGGGCTCTGTTCCGGCTTTGGACCTCCCCAAGCTCGAGCAGTTCAACATTTCGAACAATTTGCTAACTGGGTCTATTCCGAAGAAGTTACGTTCTTACAAATCGAGCTCGTTTCTGGGTAACTCACTGTGTGGAGGACCTCTTGGTGCTTGTCCAGGTGAGGTGGATAATGGAGACTTCAATTTCGACGGTAGCAAGAAGAAGAAAAAGCTCTCCGGTGGAGCTATTGCCGGTATTGTGATTGGATCTGTGGTTGGTTTTGTTGTGATCGTTGCGATTTTGTTCCTTCTGTGCCGGAAGAAGAGCAGCAAGAAGACCAGCTCAGTCGACATTGCCAGGACAGTGAAGCACCCGGAGGTCGAGATTCCAGGCGAAAAGCTGCCGGAGCTGGAGAGCGGAGGTGGTTACGGAAATGGGTACTCGGTGGGAGCGGCGGCCGCGGCGGCGATGGTGGGGAATGGGAAGAGTGAAGCTGCTAATGCTAGTGGTGCTGCTGCTGGAGCTAAGAAGTTGATATTTTTCGGAAATGGGCCGAGGGTGTTTGATCTGGAGGACTTGTTGAGAGCCTCGGCGGAGGTGTTGGGGAAAGGGACGTTTGGGACGGCGTACAAGGCGGTTTTGGAGGCCGGGACGGTGGTGGCGGTGAAGAGGCTTAAGGATGTGACCATTACAGAGAAGGAGTTTAAGGAAAAGATTGAATCTGTTGGAGCTATGGATCATGAGAGTTTGGTTCCTTTGAGAGCTTATTATTTCAGCAGAGATGAGAAGCTTCTTGTCTATGATTACATGCCCATGGGAAGCTTATCTGCGCTTTTGCATGGAAACAAAGGAGCAGGTAGAACTCCACTGAACTGGGAAATCAGGTCCGGCATTGCCCTTGGAGCTGCCCGTGGAATTGAATACCTACACTCTCAAGGCCCCAATGTCTCTCACGGAAACATAAAGTCGTCCAACATCCTGCTTACAAAATCCTATGAAGGTCGGGTGTCTGATTTCGGCTTAGCACACCTTGTTGGACCCTCTTCCACTCCCAACAGAGTTGCTGGCTACCGAGCACCAGAGGTGACTGATCCTCGCAAGGTTTCACAGAAGGCCGATGTTTACAGCTTTGGGGTACTGCTCTTGGAGCTACTCACCGGGAAGCCCCCAACCCATGCTCTCTTGAATGAGGAAGGAGTTGACCTGCCAAGGTGGGTTCAGTCCATAGTTAAAGAAGAGTGGACTTCTGAGGTTTTCGATCTTGAGCTTCTGAGGTACCAGAACGTCGAGGAGGAGATGGTTCAGCTATTGCAACTTGCGATAGATTGTTCAGCACAGTATCCTGATAAACGCCCTTCAATATCCGAAGTGACAAGGCGCATTGTGGAGCTACGCCGTTCTACATTGCGGGAAGATCAGCCCAATGCTGTCCATGATATTGATGATGGGTCTTCCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

657

Amino Acids

70.96

Weight (kDa)

8.56

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 65	1.3e-07	Leucine rich repeat N-terminal domain
Pkinase	PF00069	369 - 628	9.2e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	369 - 630	3.8e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002683)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48480
fragaria_vesca	FvH4_2g12190 FvH4_2g12190 FvH4_5g17570
malus_domestica	MD02G1259100.v1.1 MD06G1231600.v1.1 MD09G1086600.v1.1 MD09G1271200.v1.1 MD14G1238300.v1.1 MD17G1269300.v1.1
prunus_persica	Prupe.3G017300_v2.0.a1 Prupe.5G235500_v2.0.a1
pyrus_communis	pycom06g20550 pycom09g18220 pycom14g19950 pycom17g26800
rosa_chinensis	RchiOBHm_Chr3g0491091 RchiOBHm_Chr7g0178081
rosa_laevigata	RLG00000005473 RLG00000022865
rosa_multiflora	Rmu_co8170494.1_g000001 Rmu_co8379533.1_g000001 Rmu_co8445967.1_g000001 Rmu_sc0000425.1_g000007 Rmu_sc0005038.1_g000014 Rmu_ssc0000386.1_g000061 Rmu_ssc0000386.1_g000063
rosa_roxburghii	Rroxscaffold_6G00393220
rosa_rugosa	Rorug03G0246000 Rorug06G0413300
rosa_samantha	Rh3AG296200 Rh3BG332200 Rh3CG329300 Rh3DG329900 Rh7AG012600 Rh7BG012500 Rh7CG012500 Rh7DG012800
rosa_wichuraiana	Rw3G026140 Rw7G000990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	206
Acc36I	ACCTGC	2 cut(s)	1355, 1722
Acc65I	GGTACC	1 cut(s)	1785
AccB1I	GGYRCC	2 cut(s)	245, 1785
AccBSI	CCGCTC	3 cut(s)	120, 927, 962
AccI	GTMKAC	1 cut(s)	858
AccII	CGCG	3 cut(s)	99, 207, 971
AclWI	GGATC	3 cut(s)	784, 1254, 1595
AcoI	YGGCCR	1 cut(s)	966
AdeI	CACNNNGTG	1 cut(s)	662
AfaI	GTAC	6 cut(s)	413, 512, 950, 1139, 1647, 1787
AfiI	CCNNNNNNNGG	3 cut(s)	147, 148, 232
AflII	CTTAAG	1 cut(s)	1184
AgeI	ACCGGT	1 cut(s)	317
AgsI	TTSAA	7 cut(s)	484, 577, 712, 1229, 1430, 1696, 1869
AhlI	ACTAGT	1 cut(s)	55
AjiI	CACGTC	1 cut(s)	154
AjnI	CCWGG	5 cut(s)	176, 226, 685, 866, 898
AloI	GAACNNNNNNTCC	2 cut(s)	371, 403
Alw21I	GWGCWC	3 cut(s)	92, 641, 1588
Alw26I	GTCTC	4 cut(s)	227, 308, 699, 1462
AlwI	GGATC	3 cut(s)	784, 1254, 1595
Ama87I	CYCGRG	2 cut(s)	401, 566
ApeKI	GCWGC	6 cut(s)	837, 910, 1004, 1022, 1025, 1415
ArsI	GACNNNNNNTTYG	4 cut(s)	10, 42, 698, 730
AsiGI	ACCGGT	1 cut(s)	317
Asp700I	GAANNNNTTC	1 cut(s)	383
Asp718I	GGTACC	1 cut(s)	1785
AspLEI	GCGC	3 cut(s)	101, 1342, 1890
AsuC2I	CCSGG	4 cut(s)	160, 884, 1159, 1669
AsuHPI	GGTGA	6 cut(s)	307, 465, 701, 1186, 1606, 1657
AsuII	TTCGAA	1 cut(s)	584
AvaI	CYCGRG	2 cut(s)	401, 566
AvaII	GGWCC	7 cut(s)	138, 320, 430, 553, 668, 1395, 1547
BanI	GGYRCC	2 cut(s)	245, 1785
BanII	GRGCYC	3 cut(s)	92, 539, 641
BbsI	GAAGAC	3 cut(s)	290, 851, 1953
Bbv12I	GWGCWC	3 cut(s)	92, 641, 1588
BbvI	GCAGC	6 cut(s)	849, 897, 991, 1009, 1012, 1402
BccI	CCATC	3 cut(s)	973, 1801, 1949
BceAI	ACGGC	4 cut(s)	110, 259, 1149, 1890
BcgI	CGANNNNNNTGC	2 cut(s)	574, 608
BciT130I	CCWGG	5 cut(s)	178, 228, 687, 868, 900
BciVI	GTATCC	1 cut(s)	1860
BcnI	CCSGG	4 cut(s)	160, 884, 1159, 1669
BcoDI	GTCTC	4 cut(s)	227, 308, 699, 1462
BcuI	ACTAGT	1 cut(s)	55
BfaI	CTAG	2 cut(s)	56, 1014
BfrI	CTTAAG	1 cut(s)	1184
BfuAI	ACCTGC	2 cut(s)	1355, 1722
BfuI	GTATCC	1 cut(s)	1860
BglI	GCCNNNNNGGC	1 cut(s)	974
BglII	AGATCT	3 cut(s)	82, 334, 468
BlpI	GCTNAGC	1 cut(s)	1531
Bme1390I	CCNGG	9 cut(s)	160, 178, 228, 687, 868, 884, 900, 1159, 1669
Bme18I	GGWCC	7 cut(s)	138, 320, 430, 553, 668, 1395, 1547
BmeT110I	CYCGRG	2 cut(s)	401, 566
BmgBI	CACGTC	1 cut(s)	154
BmiI	GGNNCC	6 cut(s)	140, 247, 1263, 1450, 1549, 1787
BmrFI	CCNGG	9 cut(s)	160, 178, 228, 687, 868, 884, 900, 1159, 1669
BmrI	ACTGGG	2 cut(s)	609, 1393
BmuI	ACTGGG	2 cut(s)	609, 1393
BpiI	GAAGAC	3 cut(s)	290, 851, 1953
BpmI	CTGGAG	3 cut(s)	941, 1050, 1100
Bpu1102I	GCTNAGC	1 cut(s)	1531
Bpu14I	TTCGAA	1 cut(s)	584
BpuEI	CTTGAG	2 cut(s)	1428, 1793
BpuMI	CCSGG	4 cut(s)	160, 884, 1159, 1669
BsaWI	WCCGGW	2 cut(s)	317, 746
BsaXI	ACNNNNNCTCC	8 cut(s)	371, 401, 431, 443, 461, 473, 1073, 1103
Bsc4I	CCNNNNNNNGG	3 cut(s)	147, 148, 232
Bse118I	RCCGGY	3 cut(s)	278, 317, 761
Bse1I	ACTGG	3 cut(s)	262, 604, 1388
Bse3DI	GCAATG	3 cut(s)	861, 1401, 1913
BseBI	CCWGG	5 cut(s)	178, 228, 687, 868, 900
BseGI	GGATG	2 cut(s)	1195, 1485
BseLI	CCNNNNNNNGG	3 cut(s)	147, 148, 232
BseMI	GCAATG	3 cut(s)	861, 1401, 1913
BseMII	CTCAG	5 cut(s)	77, 307, 867, 1749, 1772
BseNI	ACTGG	3 cut(s)	262, 604, 1388
BseRI	GAGGAG	3 cut(s)	134, 1814, 1817
BseX3I	CGGCCG	1 cut(s)	966
BseXI	GCAGC	6 cut(s)	849, 897, 991, 1009, 1012, 1402
BseYI	CCCAGC	1 cut(s)	164
Bsh1236I	CGCG	3 cut(s)	99, 207, 971
Bsh1285I	CGRYCG	2 cut(s)	97, 969
BshNI	GGYRCC	2 cut(s)	245, 1785
BshTI	ACCGGT	1 cut(s)	317
BsiEI	CGRYCG	2 cut(s)	97, 969
BsiHKAI	GWGCWC	3 cut(s)	92, 641, 1588
BsiHKCI	CYCGRG	2 cut(s)	401, 566
BslFI	GGGAC	4 cut(s)	164, 1135, 1144, 1174
BslI	CCNNNNNNNGG	3 cut(s)	147, 148, 232
BsmAI	GTCTC	4 cut(s)	227, 308, 699, 1462
BsmBI	CGTCTC	2 cut(s)	227, 308
BsmFI	GGGAC	4 cut(s)	164, 1135, 1144, 1174
BsmI	GAATGC	1 cut(s)	4
BsoBI	CYCGRG	2 cut(s)	401, 566
Bsp119I	TTCGAA	1 cut(s)	584
Bsp1286I	GDGCHC	4 cut(s)	92, 539, 641, 1588
Bsp1407I	TGTACA	1 cut(s)	411
Bsp1720I	GCTNAGC	1 cut(s)	1531
Bsp19I	CCATGG	1 cut(s)	1323
BspCNI	CTCAG	5 cut(s)	78, 306, 866, 1750, 1773
BspFNI	CGCG	3 cut(s)	99, 207, 971
BspHI	TCATGA	1 cut(s)	1249
BspLI	GGNNCC	6 cut(s)	140, 247, 1263, 1450, 1549, 1787
BspMI	ACCTGC	2 cut(s)	1355, 1722
BspPI	GGATC	3 cut(s)	784, 1254, 1595
BspQI	GCTCTTC	1 cut(s)	827
BspT104I	TTCGAA	1 cut(s)	584
BspT107I	GGYRCC	2 cut(s)	245, 1785
BspTI	CTTAAG	1 cut(s)	1184
BsrBI	CCGCTC	3 cut(s)	120, 927, 962
BsrDI	GCAATG	3 cut(s)	861, 1401, 1913
BsrFI	RCCGGY	3 cut(s)	278, 317, 761
BsrGI	TGTACA	1 cut(s)	411
BsrI	ACTGG	3 cut(s)	262, 604, 1388
BssAI	RCCGGY	3 cut(s)	278, 317, 761
BssT1I	CCWWGG	3 cut(s)	1323, 1408, 1718
Bst2UI	CCWGG	5 cut(s)	178, 228, 687, 868, 900
Bst4CI	ACNGT	7 cut(s)	214, 510, 660, 722, 874, 1165, 1848
Bst6I	CTCTTC	6 cut(s)	360, 827, 989, 1172, 1558, 1740
BstAFI	CTTAAG	1 cut(s)	1184
BstAUI	TGTACA	1 cut(s)	411
BstBI	TTCGAA	1 cut(s)	584
BstC8I	GCNNGC	3 cut(s)	346, 419, 1576
BstDEI	CTNAG	7 cut(s)	86, 293, 853, 1035, 1531, 1758, 1781
BstDSI	CCRYGG	4 cut(s)	241, 969, 1323, 1420
BstF5I	GGATG	2 cut(s)	1195, 1485
BstFNI	CGCG	3 cut(s)	99, 207, 971
BstHHI	GCGC	3 cut(s)	101, 1342, 1890
BstMAI	GTCTC	4 cut(s)	227, 308, 699, 1462
BstMCI	CGRYCG	2 cut(s)	97, 969
BstNI	CCWGG	5 cut(s)	178, 228, 687, 868, 900
BstNSI	RCATGY	1 cut(s)	1321
BstSCI	CCNGG	9 cut(s)	158, 176, 226, 685, 866, 882, 898, 1157, 1667
BstUI	CGCG	3 cut(s)	99, 207, 971
BstV1I	GCAGC	6 cut(s)	849, 897, 991, 1009, 1012, 1402
BstV2I	GAAGAC	3 cut(s)	290, 851, 1953
BstX2I	RGATCY	4 cut(s)	82, 334, 468, 776
BstXI	CCANNNNNNTGG	2 cut(s)	177, 1383
BstYI	RGATCY	4 cut(s)	82, 334, 468, 776
BstZI	CGGCCG	1 cut(s)	966
BsuI	GTATCC	1 cut(s)	1860
BtgI	CCRYGG	4 cut(s)	241, 969, 1323, 1420
BtgZI	GCGATG	1 cut(s)	992
BtrI	CACGTC	1 cut(s)	154
BtsCI	GGATG	2 cut(s)	1195, 1485
BtsIMutI	CAGTG	6 cut(s)	210, 255, 506, 656, 879, 1376
BveI	ACCTGC	2 cut(s)	1355, 1722
Cac8I	GCNNGC	3 cut(s)	346, 419, 1576
CciI	TCATGA	1 cut(s)	1249
CciNI	GCGGCCGC	1 cut(s)	966
CfoI	GCGC	3 cut(s)	101, 1342, 1890
Cfr10I	RCCGGY	3 cut(s)	278, 317, 761
Cfr42I	CCGCGG	1 cut(s)	972
CseI	GACGC	1 cut(s)	196
Csp6I	GTAC	6 cut(s)	412, 511, 949, 1138, 1646, 1786
CspAI	ACCGGT	1 cut(s)	317
CviAII	CATG	8 cut(s)	171, 422, 1250, 1318, 1324, 1349, 1685, 1943
CviQI	GTAC	6 cut(s)	412, 511, 949, 1138, 1646, 1786
DdeI	CTNAG	7 cut(s)	86, 293, 853, 1035, 1531, 1758, 1781
DraIII	CACNNNGTG	1 cut(s)	662
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
EaeI	YGGCCR	1 cut(s)	966
EagI	CGGCCG	1 cut(s)	966
Eam1104I	CTCTTC	6 cut(s)	360, 827, 989, 1172, 1558, 1740
EarI	CTCTTC	6 cut(s)	360, 827, 989, 1172, 1558, 1740
EciI	GGCGGA	3 cut(s)	112, 331, 1118
Ecl136II	GAGCTC	2 cut(s)	90, 639
EclXI	CGGCCG	1 cut(s)	966
Eco130I	CCWWGG	3 cut(s)	1323, 1408, 1718
Eco24I	GRGCYC	3 cut(s)	92, 539, 641
Eco47I	GGWCC	7 cut(s)	138, 320, 430, 553, 668, 1395, 1547
Eco52I	CGGCCG	1 cut(s)	966
Eco53kI	GAGCTC	2 cut(s)	90, 639
Eco88I	CYCGRG	2 cut(s)	401, 566
EcoICRI	GAGCTC	2 cut(s)	90, 639
EcoO109I	RGGNCCY	2 cut(s)	668, 1448
EcoRII	CCWGG	5 cut(s)	176, 226, 685, 866, 898
EcoT14I	CCWWGG	3 cut(s)	1323, 1408, 1718
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	92, 539, 641
ErhI	CCWWGG	3 cut(s)	1323, 1408, 1718
Esp3I	CGTCTC	2 cut(s)	227, 308
FaeI	CATG	8 cut(s)	174, 425, 1253, 1321, 1327, 1352, 1688, 1946
FaqI	GGGAC	4 cut(s)	164, 1135, 1144, 1174
FatI	CATG	8 cut(s)	170, 421, 1249, 1317, 1323, 1348, 1684, 1942
FauI	CCCGC	1 cut(s)	1911
FblI	GTMKAC	1 cut(s)	858
FokI	GGATG	2 cut(s)	1202, 1472
FriOI	GRGCYC	3 cut(s)	92, 539, 641
FspBI	CTAG	2 cut(s)	56, 1014
GlaI	GCGC	3 cut(s)	100, 1341, 1889
GsaI	CCCAGC	1 cut(s)	168
GsuI	CTGGAG	3 cut(s)	941, 1050, 1100
HgaI	GACGC	1 cut(s)	196
HhaI	GCGC	3 cut(s)	101, 1342, 1890
Hin1II	CATG	8 cut(s)	174, 425, 1253, 1321, 1327, 1352, 1688, 1946
Hin6I	GCGC	3 cut(s)	99, 1340, 1888
HinP1I	GCGC	3 cut(s)	99, 1340, 1888
HincII	GTYRAC	3 cut(s)	190, 859, 1711
HindII	GTYRAC	3 cut(s)	190, 859, 1711
HindIII	AAGCTT	3 cut(s)	527, 1298, 1330
HinfI	GANTC	4 cut(s)	25, 186, 895, 1229
HphI	GGTGA	6 cut(s)	307, 465, 701, 1186, 1606, 1657
Hpy166II	GTNNAC	5 cut(s)	190, 859, 1633, 1711, 1752
Hpy188III	TCNNGA	7 cut(s)	892, 1079, 1250, 1693, 1772, 1853, 1965
Hpy8I	GTNNAC	5 cut(s)	190, 859, 1633, 1711, 1752
Hpy99I	CGWCG	3 cut(s)	131, 722, 1799
HpyAV	CCTTC	9 cut(s)	116, 293, 486, 827, 1201, 1502, 1615, 1697, 1875
HpyCH4III	ACNGT	7 cut(s)	214, 510, 660, 722, 874, 1165, 1848
HpyCH4IV	ACGT	4 cut(s)	153, 622, 1124, 1794
HpyCH4V	TGCA	4 cut(s)	4, 421, 1348, 1822
HpyF3I	CTNAG	7 cut(s)	86, 293, 853, 1035, 1531, 1758, 1781
HpySE526I	ACGT	4 cut(s)	153, 622, 1124, 1794
Hsp92II	CATG	8 cut(s)	174, 425, 1253, 1321, 1327, 1352, 1688, 1946
HspAI	GCGC	3 cut(s)	99, 1340, 1888
KpnI	GGTACC	1 cut(s)	1789
KspI	CCGCGG	1 cut(s)	972
LguI	GCTCTTC	1 cut(s)	827
Lsp1109I	GCAGC	6 cut(s)	849, 897, 991, 1009, 1012, 1402
MaeI	CTAG	2 cut(s)	56, 1014
MaeII	ACGT	4 cut(s)	153, 622, 1124, 1794
MbiI	CCGCTC	3 cut(s)	120, 927, 962
MflI	RGATCY	4 cut(s)	82, 334, 468, 776
MhlI	GDGCHC	4 cut(s)	92, 539, 641, 1588
MluCI	AATT	3 cut(s)	589, 712, 1425
MlyI	GAGTC	2 cut(s)	34, 195
MmeI	TCCRAC	6 cut(s)	109, 117, 474, 1216, 1506, 1525
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	383
MseI	TTAA	3 cut(s)	1185, 1215, 1740
MslI	CAYNNNNRTG	1 cut(s)	1322
MspA1I	CMGCKG	1 cut(s)	971
MspCI	CTTAAG	1 cut(s)	1184
MspR9I	CCNGG	9 cut(s)	160, 178, 228, 687, 868, 884, 900, 1159, 1669
Mva1269I	GAATGC	1 cut(s)	4
MvaI	CCWGG	5 cut(s)	178, 228, 687, 868, 900
MvnI	CGCG	3 cut(s)	99, 207, 971
NciI	CCSGG	4 cut(s)	160, 884, 1159, 1669
NcoI	CCATGG	1 cut(s)	1323
NlaIII	CATG	8 cut(s)	174, 425, 1253, 1321, 1327, 1352, 1688, 1946
NlaIV	GGNNCC	6 cut(s)	140, 247, 1263, 1450, 1549, 1787
NmeAIII	GCCGAG	2 cut(s)	1079, 1089
NmuCI	GTSAC	5 cut(s)	154, 208, 1192, 1594, 1879
NotI	GCGGCCGC	1 cut(s)	966
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	1 cut(s)	1321
NspV	TTCGAA	1 cut(s)	584
PaeR7I	CTCGAG	1 cut(s)	566
PagI	TCATGA	1 cut(s)	1249
PciSI	GCTCTTC	1 cut(s)	827
PctI	GAATGC	1 cut(s)	4
PdmI	GAANNNNTTC	1 cut(s)	383
PfeI	GAWTC	2 cut(s)	895, 1229
PflFI	GACNNNGTC	1 cut(s)	428
PinAI	ACCGGT	1 cut(s)	317
PleI	GAGTC	2 cut(s)	33, 194
PpsI	GAGTC	2 cut(s)	33, 194
PpuMI	RGGWCCY	1 cut(s)	668
Psp124BI	GAGCTC	2 cut(s)	92, 641
Psp5II	RGGWCCY	1 cut(s)	668
Psp6I	CCWGG	5 cut(s)	176, 226, 685, 866, 898
PspFI	CCCAGC	1 cut(s)	164
PspGI	CCWGG	5 cut(s)	176, 226, 685, 866, 898
PspN4I	GGNNCC	6 cut(s)	140, 247, 1263, 1450, 1549, 1787
PspPPI	RGGWCCY	1 cut(s)	668
PspXI	VCTCGAGB	1 cut(s)	566
PsuI	RGATCY	4 cut(s)	82, 334, 468, 776
PsyI	GACNNNGTC	1 cut(s)	428
RsaI	GTAC	6 cut(s)	413, 512, 950, 1139, 1647, 1787
RsaNI	GTAC	6 cut(s)	412, 511, 949, 1138, 1646, 1786
RseI	CAYNNNNRTG	1 cut(s)	1322
SacI	GAGCTC	2 cut(s)	92, 641
SacII	CCGCGG	1 cut(s)	972
SalI	GTCGAC	1 cut(s)	857
SapI	GCTCTTC	1 cut(s)	827
SaqAI	TTAA	3 cut(s)	1185, 1215, 1740
SchI	GAGTC	2 cut(s)	34, 195
ScrFI	CCNGG	9 cut(s)	160, 178, 228, 687, 868, 884, 900, 1159, 1669
SduI	GDGCHC	4 cut(s)	92, 539, 641, 1588
Sfr274I	CTCGAG	1 cut(s)	566
Sfr303I	CCGCGG	1 cut(s)	972
SfuI	TTCGAA	1 cut(s)	584
SgrBI	CCGCGG	1 cut(s)	972
SinI	GGWCC	7 cut(s)	138, 320, 430, 553, 668, 1395, 1547
SlaI	CTCGAG	1 cut(s)	566
SmiMI	CAYNNNNRTG	1 cut(s)	1322
SmlI	CTYRAG	4 cut(s)	566, 1184, 1443, 1772
SmoI	CTYRAG	4 cut(s)	566, 1184, 1443, 1772
SpeI	ACTAGT	1 cut(s)	55
Sse9I	AATT	3 cut(s)	589, 712, 1425
SspMI	CTAG	2 cut(s)	56, 1014
SstI	GAGCTC	2 cut(s)	92, 641
StyD4I	CCNGG	9 cut(s)	158, 176, 226, 685, 866, 882, 898, 1157, 1667
StyI	CCWWGG	3 cut(s)	1323, 1408, 1718
TaaI	ACNGT	7 cut(s)	214, 510, 660, 722, 874, 1165, 1848
TaiI	ACGT	4 cut(s)	156, 625, 1127, 1797
TaqI	TCGA	9 cut(s)	516, 567, 584, 635, 717, 858, 891, 1767, 1797
TasI	AATT	3 cut(s)	589, 712, 1425
TatI	WGTACW	1 cut(s)	411
TauI	GCSGC	6 cut(s)	104, 965, 968, 971, 974, 977
TfiI	GAWTC	2 cut(s)	895, 1229
Tru1I	TTAA	3 cut(s)	1185, 1215, 1740
Tru9I	TTAA	3 cut(s)	1185, 1215, 1740
TscAI	CASTG	6 cut(s)	217, 262, 513, 663, 879, 1383
TseFI	GTSAC	5 cut(s)	154, 208, 1192, 1594, 1879
TseI	GCWGC	6 cut(s)	837, 910, 1004, 1022, 1025, 1415
Tsp45I	GTSAC	5 cut(s)	154, 208, 1192, 1594, 1879
TspDTI	ATGAA	1 cut(s)	1521
TspGWI	ACGGA	2 cut(s)	954, 1479
TspRI	CASTG	6 cut(s)	217, 262, 513, 663, 879, 1383
Tth111I	GACNNNGTC	1 cut(s)	428
Vha464I	CTTAAG	1 cut(s)	1184
VpaK11BI	GGWCC	7 cut(s)	138, 320, 430, 553, 668, 1395, 1547
XceI	RCATGY	1 cut(s)	1321
XhoI	CTCGAG	1 cut(s)	566
XmiI	GTMKAC	1 cut(s)	858
XmnI	GAANNNNTTC	1 cut(s)	383
XspI	CTAG	2 cut(s)	56, 1014
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.