Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008160

Physical Location & Seq

Genomic Coordinates Reverse (-)

5927063 .. 5928765

Gene Structure 1703 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1063200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 381 bp

ATGTCGGGTTTGGCAAAAGCAAATTTTAGTGAATATCGACAATTGACAGCAGATGTCGATAACAGTGGAACCATTGATTATGGGGAGTTCGTAGCTGCAACGTTGCATCTGAACAAAATTGAGAGAGAAGATCATCTATTTGCAGCTTTCTCCTACTTTGATAAGGATGGAAGTGGCTATATTACTCCGGATGAGCTTCAACAAGCTTGTGAGGATTTTGGCATAGAGGATGTTCGCCTCGAAGAGATGATAAGAGAAGTTGATCAGGACAATGATGGACGCATAGATTACAACGAGTTTGTAGCCATGATGCAGAAGGGAAATTTTGTTGGCCCTGGTAAGAAGGGACTGCAAAGTAGCTTCAGCATTGCATTTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005509 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005778 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031090 GO:0031903 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042579 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

127

Amino Acids

14.21

Weight (kDa)

4.39

Isoelectric Point (pI)

33.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	43 - 105	4e-17	EF-hand domain pair
EF-hand_1	PF00036	47 - 71	2.8e-07	EF hand domain
EF-hand_6	PF13405	47 - 74	6.5e-08	EF-hand domain
EF-hand_5	PF13202	49 - 68	6.1e-06	EF hand
EF-hand_1	PF00036	80 - 106	8.4e-10	EF hand domain
EF-hand_8	PF13833	81 - 106	8e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0008160)

Species	Orthologous Gene IDs
malus_domestica	MD07G1063200.v1.1
pyrus_communis	pycom02g21820 pycom02g21840
rosa_chinensis	RchiOBHm_Chr3g0470181
rosa_laevigata	RLG00000008106 RLG00000029152 RLG00000031580 RLG00000034936
rosa_multiflora	Rmu_co8301435.1_g000001 Rmu_co8456623.1_g000001 Rmu_sc0002799.1_g000014 Rmu_sc0014039.1_g000005
rosa_roxburghii	Rroxscaffold_3G00272930
rosa_rugosa	Rorug02G0162400 Rorug04G0088700
rosa_samantha	Rh1AG124000 Rh2BG466300 Rh3BG244000 Rh3CG171600 Rh4AG140600 Rh5BG342900 Rh5CG369900 Rh6AG182300 Rh7BG390200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	187
AclI	AACGTT	1 cut(s)	101
AcsI	RAATTY	2 cut(s)	22, 322
AcuI	CTGAAG	1 cut(s)	346
AgsI	TTSAA	1 cut(s)	200
AjnI	CCWGG	1 cut(s)	334
AluBI	AGCT	5 cut(s)	95, 146, 196, 206, 360
AluI	AGCT	5 cut(s)	95, 146, 196, 206, 360
Aor13HI	TCCGGA	1 cut(s)	187
AoxI	GGCC	1 cut(s)	331
ApeKI	GCWGC	2 cut(s)	95, 143
ApoI	RAATTY	2 cut(s)	22, 322
AspS9I	GGNCC	1 cut(s)	332
BbvI	GCAGC	2 cut(s)	82, 155
BccI	CCATC	2 cut(s)	161, 269
BciT130I	CCWGG	1 cut(s)	336
BclI	TGATCA	1 cut(s)	262
BisI	GCNGC	2 cut(s)	96, 144
BlsI	GCNGC	2 cut(s)	97, 145
Bme1390I	CCNGG	1 cut(s)	336
BmgT120I	GGNCC	1 cut(s)	332
BmiI	GGNNCC	1 cut(s)	70
BmrFI	CCNGG	1 cut(s)	336
BmsI	GCATC	2 cut(s)	115, 300
BsaJI	CCNNGG	1 cut(s)	334
BsaWI	WCCGGW	1 cut(s)	187
Bse3DI	GCAATG	1 cut(s)	366
BseAI	TCCGGA	1 cut(s)	187
BseBI	CCWGG	1 cut(s)	336
BseDI	CCNNGG	1 cut(s)	334
BseGI	GGATG	3 cut(s)	172, 196, 235
BseMI	GCAATG	1 cut(s)	366
BseXI	GCAGC	2 cut(s)	82, 155
BshFI	GGCC	1 cut(s)	333
BsiSI	CCGG	1 cut(s)	188
BslFI	GGGAC	1 cut(s)	360
BsmFI	GGGAC	1 cut(s)	360
BsnI	GGCC	1 cut(s)	333
Bsp13I	TCCGGA	1 cut(s)	187
Bsp143I	GATC	2 cut(s)	130, 262
BspANI	GGCC	1 cut(s)	333
BspEI	TCCGGA	1 cut(s)	187
BspLI	GGNNCC	1 cut(s)	70
BsrDI	GCAATG	1 cut(s)	366
BssECI	CCNNGG	1 cut(s)	334
BssMI	GATC	2 cut(s)	130, 262
Bst2UI	CCWGG	1 cut(s)	336
Bst4CI	ACNGT	1 cut(s)	65
Bst6I	CTCTTC	1 cut(s)	237
BstF5I	GGATG	3 cut(s)	172, 196, 235
BstKTI	GATC	2 cut(s)	133, 265
BstMBI	GATC	2 cut(s)	130, 262
BstNI	CCWGG	1 cut(s)	336
BstSCI	CCNGG	1 cut(s)	334
BstV1I	GCAGC	2 cut(s)	82, 155
BsuRI	GGCC	1 cut(s)	333
BtsCI	GGATG	3 cut(s)	172, 196, 235
BtsIMutI	CAGTG	1 cut(s)	70
Cfr13I	GGNCC	1 cut(s)	332
CseI	GACGC	1 cut(s)	288
CviAII	CATG	1 cut(s)	307
CviJI	RGCY	8 cut(s)	95, 146, 177, 196, 206, 305, 333, 360
CviKI_1	RGCY	8 cut(s)	95, 146, 177, 196, 206, 305, 333, 360
DpnI	GATC	2 cut(s)	132, 264
DpnII	GATC	2 cut(s)	130, 262
Eam1104I	CTCTTC	1 cut(s)	237
EarI	CTCTTC	1 cut(s)	237
Eco57I	CTGAAG	1 cut(s)	346
EcoRII	CCWGG	1 cut(s)	334
FaeI	CATG	1 cut(s)	310
FaiI	YATR	5 cut(s)	81, 180, 224, 284, 308
FaqI	GGGAC	1 cut(s)	360
FatI	CATG	1 cut(s)	306
FbaI	TGATCA	1 cut(s)	262
Fnu4HI	GCNGC	2 cut(s)	96, 144
FokI	GGATG	3 cut(s)	179, 203, 242
Fsp4HI	GCNGC	2 cut(s)	96, 144
GluI	GCNGC	2 cut(s)	96, 144
HaeIII	GGCC	1 cut(s)	333
HapII	CCGG	1 cut(s)	188
HgaI	GACGC	1 cut(s)	288
Hin1II	CATG	1 cut(s)	310
HindIII	AAGCTT	1 cut(s)	204
HpaII	CCGG	1 cut(s)	188
Hpy188I	TCNGA	1 cut(s)	111
Hpy188III	TCNNGA	2 cut(s)	188, 266
HpyAV	CCTTC	2 cut(s)	310, 337
HpyCH4III	ACNGT	1 cut(s)	65
HpyCH4IV	ACGT	1 cut(s)	101
HpyCH4V	TGCA	6 cut(s)	98, 106, 143, 313, 352, 371
HpySE526I	ACGT	1 cut(s)	101
Hsp92II	CATG	1 cut(s)	310
Kpn2I	TCCGGA	1 cut(s)	187
Ksp22I	TGATCA	1 cut(s)	262
Kzo9I	GATC	2 cut(s)	130, 262
LpnPI	CCDG	4 cut(s)	201, 251, 321, 348
Lsp1109I	GCAGC	2 cut(s)	82, 155
LweI	GCATC	2 cut(s)	115, 300
MaeII	ACGT	1 cut(s)	101
MalI	GATC	2 cut(s)	132, 264
MboI	GATC	2 cut(s)	130, 262
MboII	GAAGA	2 cut(s)	140, 254
MfeI	CAATTG	1 cut(s)	41
MluCI	AATT	4 cut(s)	22, 41, 117, 322
MnlI	CCTC	3 cut(s)	205, 220, 248
MroI	TCCGGA	1 cut(s)	187
MspI	CCGG	1 cut(s)	188
MspR9I	CCNGG	1 cut(s)	336
MunI	CAATTG	1 cut(s)	41
MvaI	CCWGG	1 cut(s)	336
NdeII	GATC	2 cut(s)	130, 262
NlaIII	CATG	1 cut(s)	310
NlaIV	GGNNCC	1 cut(s)	70
PkrI	GCNGC	2 cut(s)	97, 145
Psp1406I	AACGTT	1 cut(s)	101
Psp6I	CCWGG	1 cut(s)	334
PspGI	CCWGG	1 cut(s)	334
PspN4I	GGNNCC	1 cut(s)	70
PspPI	GGNCC	1 cut(s)	332
SatI	GCNGC	2 cut(s)	96, 144
Sau3AI	GATC	2 cut(s)	130, 262
Sau96I	GGNCC	1 cut(s)	332
ScrFI	CCNGG	1 cut(s)	336
SetI	ASST	6 cut(s)	97, 104, 148, 198, 208, 362
SfaNI	GCATC	2 cut(s)	115, 300
Sse9I	AATT	4 cut(s)	22, 41, 117, 322
StyD4I	CCNGG	1 cut(s)	334
TaaI	ACNGT	1 cut(s)	65
TaiI	ACGT	1 cut(s)	104
TaqI	TCGA	3 cut(s)	37, 57, 240
TasI	AATT	4 cut(s)	22, 41, 117, 322
TscAI	CASTG	1 cut(s)	70
TseI	GCWGC	2 cut(s)	95, 143
TspRI	CASTG	1 cut(s)	70
XapI	RAATTY	2 cut(s)	22, 322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.