Rh7BG390200

acetyltransferase component of pyruvate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008160
Physical Location & Seq
Reverse (-)
42820646 .. 42822882
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG390200.1

Sequence Viewer

Length: 354 bp
ATGCTGATGGAAGTGGTTGATGTTGATGGGAATCGATTTCCGGATTATCGAGAGTCCGCAGCAGTTACAATTCACTTGCAAAAATTGGACATTGATGAACATCTTTGGAGAGCATTTGCATTGGGTTGTATACCTATTACTCAGTGGCTTGCTGATGTGACCATGACTGCCTTTCCTGCCAAGGCACTTGCTAAGGCACTTGCTCAGCACCCAGTTGTAAATGCCAGCTGCAGATATGAAAAAAGCTTTTACTACAACAGTAGCATAAACATCGCCATTGCTGCAGATAAGAAATGGAAAGAGCTGACGGAGAAGGATGGAGAAGGCTCGCTCAAACCAACTTCAGCCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

117

Amino Acids

13.05

Weight (kDa)

5.87

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-oxoacid_dh PF00198 52 - 97 1.8e-07 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 327
AluBI AGCT 3 cut(s) 228, 246, 304
AluI AGCT 3 cut(s) 228, 246, 304
Aor13HI TCCGGA 1 cut(s) 40
ApeKI GCWGC 3 cut(s) 59, 228, 281
BbvI GCAGC 3 cut(s) 71, 215, 268
BccI CCATC 2 cut(s) 20, 311
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BfmI CTRYAG 2 cut(s) 229, 282
BisI GCNGC 3 cut(s) 60, 229, 282
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 3 cut(s) 61, 230, 283
BmrI ACTGGG 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 204
Bsa29I ATCGAT 1 cut(s) 34
BsaBI GATNNNNATC 2 cut(s) 30, 99
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 2 cut(s) 30, 99
BseAI TCCGGA 1 cut(s) 40
BseCI ATCGAT 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 1 cut(s) 322
BseJI GATNNNNATC 2 cut(s) 30, 99
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 155, 218
BseNI ACTGG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 71, 215, 268
BshVI ATCGAT 1 cut(s) 34
BsiSI CCGG 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 40
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 1 cut(s) 57
BspCNI CTCAG 2 cut(s) 154, 217
BspDI ATCGAT 1 cut(s) 34
BspEI TCCGGA 1 cut(s) 40
BspMAI CTGCAG 2 cut(s) 233, 286
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 180
BssNAI GTATAC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 180
Bst1107I GTATAC 1 cut(s) 131
Bst4CI ACNGT 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 150, 226, 329
BstDEI CTNAG 3 cut(s) 141, 192, 204
BstF5I GGATG 1 cut(s) 322
BstMWI GCNNNNNNNGC 2 cut(s) 176, 281
BstSFI CTRYAG 2 cut(s) 229, 282
BstV1I GCAGC 3 cut(s) 71, 215, 268
BstZ17I GTATAC 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 34
BsuTUI ATCGAT 1 cut(s) 34
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 3 cut(s) 150, 226, 329
ClaI ATCGAT 1 cut(s) 34
CviAII CATG 1 cut(s) 163
CviJI RGCY 6 cut(s) 148, 228, 246, 304, 327, 347
CviKI_1 RGCY 6 cut(s) 148, 228, 246, 304, 327, 347
DdeI CTNAG 3 cut(s) 141, 192, 204
Eco130I CCWWGG 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 1 cut(s) 166
FaiI YATR 5 cut(s) 131, 164, 237, 266, 352
FatI CATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 60, 229, 282
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 3 cut(s) 60, 229, 282
GluI GCNGC 3 cut(s) 60, 229, 282
HapII CCGG 1 cut(s) 41
Hin1II CATG 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 2 cut(s) 31, 53
HpaII CCGG 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 131
Hpy188III TCNNGA 2 cut(s) 41, 50
Hpy8I GTNNAC 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 307, 317
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4V TGCA 4 cut(s) 79, 119, 231, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 281
HpyF3I CTNAG 3 cut(s) 141, 192, 204
Hsp92II CATG 1 cut(s) 166
Kpn2I TCCGGA 1 cut(s) 40
LpnPI CCDG 4 cut(s) 54, 189, 225, 238
Lsp1109I GCAGC 3 cut(s) 71, 215, 268
MaeIII GTNAC 2 cut(s) 64, 157
MluCI AATT 2 cut(s) 69, 83
MlyI GAGTC 1 cut(s) 62
MroI TCCGGA 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 176, 281
NlaIII CATG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 3 cut(s) 61, 230, 283
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PstI CTGCAG 2 cut(s) 233, 286
PvuII CAGCTG 1 cut(s) 228
SatI GCNGC 3 cut(s) 60, 229, 282
SchI GAGTC 1 cut(s) 62
SetI ASST 4 cut(s) 136, 230, 248, 306
SfcI CTRYAG 2 cut(s) 229, 282
Sse9I AATT 2 cut(s) 69, 83
SsiI CCGC 1 cut(s) 57
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 34, 49
TasI AATT 2 cut(s) 69, 83
TfiI GAWTC 1 cut(s) 31
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 59, 228, 281
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 2 cut(s) 111, 252
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.