MD07G1148500.v1.1

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007144
Physical Location & Seq
Reverse (-)
21711817 .. 21715922
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1148500.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGGCTTGTAGACGAAGTGTCTCCACGACAGTGACCTTTGTCGCTCGACGTTTCAACCCGTCATTTAGTCACATTCTTCATGATAACAATGAAGATACTAAACCAACTTCACCAAATTCAAGTTCAACTCCATCTGTGATCAAGAGTATTTTGCCAAGTGCACACAACTCAATCACTCGATTTTGTTTTGGTTCTCAATGTAGAGAGAGGACAGGCTCATCACTGGCTTGGAAAATGGGACTTGGCTCCTTCTCTTGTCGGTATATGTCTTCTGTAATTGGTGATGGGTCTGATAAGATTAATGACACTGGCTATTTTGCTGAGGTTATTACGGATAAAACATTGGAAGTAGTAACCTCCCAAGCCCCTGCTGTGAGTGAAGTGGCTGTTGCAGCAGCGGATTCCTTCCCCCCAGTTGCTGCCCTGCAGTACTTGATCGATGGCATCCATTCTTTTACCGGATTGAATTGGTGGGCATCAATAGCTTTAACAACTATTCTGATTCGAGGGGCTACAGTTCCACTTCTTATAGATCAACTCAGGGCCACTACACAACTTAATTTAATGAGGCCGCGCTTAGAGGAGTTAAGACAACAGATGCAAAATATGGCAACAGATCCAAATGTTATGCAAGAGGGTCAGAGACGAATGAAGGCATTGTTCAACGAATATGGAGTCACTCCGTTTTCTCAATTGAAAGGACTATTCATTCAAGGTCCCATCTTCGTCTGTTTTTTCTTAGCTATTAGAAACATGGCCGAGAAGGTTCCTTCTTTTGAAAGTGGTGGGGCTCTTTGGTTTACTAATCTCGCTACTCCCGATAGTCTTCTCATCCTTCCAGTTCTAACATCTTTGACATTCCTGATTACAGTAGAGTGCAATATGCAGGAGGGTCTGGAAGGGAATCCTATTGCCCAAACTATGAAAAACTATTCTAGAATCCTTGCTGCCATTTCAATTCCTGTGATGATGAGTTTCCCTAAGGCGCTCTTCTGTTATTGGCTGACCTCAAACATGTTCTCCCTTGCATATGGATTAGTAATTAGGCGCCCAGAGGTGAAGAGTTACCTTCGCCTTCCAGTAATTCCCGTGCCACCTCCTCCTACTGCATCCAAGCCTCATTCCTTTATGTCTTCGGCAATTGAACAAGATACTACCGTTGAATCAGAACCTTCGTTATACTCAGAGCAAACAAAGGCCACAGACACGAGAATATCGCGGACTTCAATTCTCAAGCAGAGACTTAGAATTCTGGAAAAGAAAATCAAGGAAAAAAAGAAACAAAATAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
60KD_IMP

Protein Analysis

432

Amino Acids

47.93

Weight (kDa)

9.32

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 157 - 349 4e-27 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1047
AccI GTMKAC 1 cut(s) 10
AccII CGCG 2 cut(s) 574, 1219
AciI CCGC 3 cut(s) 398, 572, 1219
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 2 cut(s) 115, 1248
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 1014
AhdI GACNNNNNGTC 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 29
AluBI AGCT 2 cut(s) 485, 743
AluI AGCT 2 cut(s) 485, 743
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 3 cut(s) 25, 637, 1234
Alw44I GTGCAC 1 cut(s) 159
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 4 cut(s) 543, 569, 756, 1197
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 4 cut(s) 392, 395, 419, 947
ApoI RAATTY 2 cut(s) 115, 1248
ArsI GACNNNNNNTTYG 2 cut(s) 660, 692
AseI ATTAAT 1 cut(s) 300
AspLEI GCGC 3 cut(s) 576, 988, 1050
AspS9I GGNCC 2 cut(s) 543, 716
AsuHPI GGTGA 3 cut(s) 102, 293, 1069
AvaII GGWCC 1 cut(s) 716
AxyI CCTNAGG 1 cut(s) 981
BaeGI GKGCMC 1 cut(s) 163
BaeI ACNNNNGTAYC 2 cut(s) 1143, 1176
BanI GGYRCC 1 cut(s) 1047
BanII GRGCYC 1 cut(s) 793
BauI CACGAG 1 cut(s) 1207
BbsI GAAGAC 3 cut(s) 261, 818, 1125
Bbv12I GWGCWC 1 cut(s) 163
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 4 cut(s) 404, 406, 407, 934
BccI CCATC 4 cut(s) 139, 278, 434, 728
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 3 cut(s) 25, 637, 1234
BfaI CTAG 1 cut(s) 936
BfmI CTRYAG 2 cut(s) 425, 513
BfoI RGCGCY 2 cut(s) 989, 1051
BisI GCNGC 5 cut(s) 393, 396, 420, 572, 948
BlsI GCNGC 5 cut(s) 394, 397, 421, 573, 949
BmcAI AGTACT 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 716
BmeRI GACNNNNNGTC 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 543, 716
BmiI GGNNCC 4 cut(s) 247, 718, 768, 1049
BmrI ACTGGG 1 cut(s) 407
BmsI GCATC 4 cut(s) 453, 485, 588, 1118
BmuI ACTGGG 1 cut(s) 407
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 3 cut(s) 261, 818, 1125
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 1217
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 1048
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 1004, 1034
Bse1I ACTGG 5 cut(s) 228, 313, 413, 839, 1079
Bse21I CCTNAGG 1 cut(s) 981
BseCI ATCGAT 1 cut(s) 438
BseGI GGATG 3 cut(s) 444, 831, 1109
BseMII CTCAG 3 cut(s) 312, 553, 1197
BseNI ACTGG 5 cut(s) 228, 313, 413, 839, 1079
BseRI GAGGAG 2 cut(s) 596, 1089
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 4 cut(s) 404, 406, 407, 934
Bsh1236I CGCG 2 cut(s) 574, 1219
BshFI GGCC 4 cut(s) 545, 571, 758, 1199
BshNI GGYRCC 1 cut(s) 1047
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 2 cut(s) 252, 702
BsmAI GTCTC 3 cut(s) 25, 637, 1234
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 2 cut(s) 252, 702
BsnI GGCC 4 cut(s) 545, 571, 758, 1199
Bsp1286I GDGCHC 2 cut(s) 163, 793
Bsp143I GATC 4 cut(s) 138, 435, 532, 616
BspACI CCGC 3 cut(s) 398, 572, 1219
BspANI GGCC 4 cut(s) 545, 571, 758, 1199
BspCNI CTCAG 3 cut(s) 313, 552, 1196
BspDI ATCGAT 1 cut(s) 438
BspFNI CGCG 2 cut(s) 574, 1219
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 4 cut(s) 247, 718, 768, 1049
BspMAI CTGCAG 1 cut(s) 429
BspPI GGATC 1 cut(s) 611
BspQI GCTCTTC 1 cut(s) 995
BspT107I GGYRCC 1 cut(s) 1047
BsrI ACTGG 5 cut(s) 228, 313, 413, 839, 1079
BssMI GATC 4 cut(s) 138, 435, 532, 616
BssNI GRCGYC 1 cut(s) 1048
BssSI CACGAG 1 cut(s) 1207
Bst2BI CACGAG 1 cut(s) 1207
Bst4CI ACNGT 4 cut(s) 31, 517, 871, 1159
Bst6I CTCTTC 2 cut(s) 995, 1055
BstACI GRCGYC 1 cut(s) 1048
BstDEI CTNAG 7 cut(s) 321, 539, 577, 739, 981, 1183, 1244
BstF5I GGATG 3 cut(s) 444, 831, 1109
BstFNI CGCG 2 cut(s) 574, 1219
BstH2I RGCGCY 2 cut(s) 989, 1051
BstHHI GCGC 3 cut(s) 576, 988, 1050
BstKTI GATC 4 cut(s) 141, 438, 535, 619
BstMAI GTCTC 3 cut(s) 25, 637, 1234
BstMBI GATC 4 cut(s) 138, 435, 532, 616
BstMWI GCNNNNNNNGC 2 cut(s) 392, 482
BstNSI RCATGY 1 cut(s) 1018
BstPAI GACNNNNGTC 1 cut(s) 38
BstSFI CTRYAG 2 cut(s) 425, 513
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 2 cut(s) 574, 1219
BstV1I GCAGC 4 cut(s) 404, 406, 407, 934
BstV2I GAAGAC 3 cut(s) 261, 818, 1125
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 981
BsuRI GGCC 4 cut(s) 545, 571, 758, 1199
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 3 cut(s) 444, 831, 1109
BtsIMutI CAGTG 3 cut(s) 36, 221, 306
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 79
CfoI GCGC 3 cut(s) 576, 988, 1050
Cfr13I GGNCC 2 cut(s) 543, 716
ClaI ATCGAT 1 cut(s) 438
Csp6I GTAC 1 cut(s) 430
CviAII CATG 3 cut(s) 80, 754, 1015
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 7 cut(s) 321, 539, 577, 739, 981, 1183, 1244
DinI GGCGCC 1 cut(s) 1049
DpnI GATC 4 cut(s) 140, 437, 534, 618
DpnII GATC 4 cut(s) 138, 435, 532, 616
DriI GACNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 2 cut(s) 995, 1055
Eam1105I GACNNNNNGTC 1 cut(s) 17
EarI CTCTTC 2 cut(s) 995, 1055
Eco24I GRGCYC 1 cut(s) 793
Eco47I GGWCC 1 cut(s) 716
Eco81I CCTNAGG 1 cut(s) 981
EcoO109I RGGNCCY 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 1248
EcoT38I GRGCYC 1 cut(s) 793
EgeI GGCGCC 1 cut(s) 1049
EheI GGCGCC 1 cut(s) 1049
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 3 cut(s) 83, 757, 1018
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FaqI GGGAC 2 cut(s) 252, 702
FatI CATG 3 cut(s) 79, 753, 1014
FauNDI CATATG 1 cut(s) 1030
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 5 cut(s) 393, 396, 420, 572, 948
FokI GGATG 3 cut(s) 431, 818, 1096
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 5 cut(s) 393, 396, 420, 572, 948
FspBI CTAG 1 cut(s) 936
GlaI GCGC 3 cut(s) 575, 987, 1049
GluI GCNGC 5 cut(s) 393, 396, 420, 572, 948
HaeII RGCGCY 2 cut(s) 989, 1051
HaeIII GGCC 4 cut(s) 545, 571, 758, 1199
HapII CCGG 1 cut(s) 459
HhaI GCGC 3 cut(s) 576, 988, 1050
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 3 cut(s) 83, 757, 1018
Hin6I GCGC 3 cut(s) 574, 986, 1048
HinP1I GCGC 3 cut(s) 574, 986, 1048
HinfI GANTC 6 cut(s) 401, 502, 675, 904, 939, 1163
HpaII CCGG 1 cut(s) 459
HphI GGTGA 3 cut(s) 102, 293, 1069
Hpy166II GTNNAC 3 cut(s) 11, 161, 801
Hpy188I TCNGA 5 cut(s) 292, 501, 642, 1168, 1186
Hpy188III TCNNGA 7 cut(s) 80, 142, 818, 862, 896, 936, 1253
Hpy8I GTNNAC 3 cut(s) 11, 161, 801
Hpy99I CGWCG 1 cut(s) 51
HpyCH4III ACNGT 4 cut(s) 31, 517, 871, 1159
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 9 cut(s) 161, 392, 427, 601, 631, 879, 886, 1028, 1109
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 482
HpyF3I CTNAG 7 cut(s) 321, 539, 577, 739, 981, 1183, 1244
HpySE526I ACGT 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 3 cut(s) 83, 757, 1018
HspAI GCGC 3 cut(s) 574, 986, 1048
KasI GGCGCC 1 cut(s) 1047
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 138, 435, 532, 616
LguI GCTCTTC 1 cut(s) 995
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 4 cut(s) 404, 406, 407, 934
LweI GCATC 4 cut(s) 453, 485, 588, 1118
MaeI CTAG 1 cut(s) 936
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 5 cut(s) 31, 68, 352, 676, 1064
MalI GATC 4 cut(s) 140, 437, 534, 618
MboI GATC 4 cut(s) 138, 435, 532, 616
MboII GAAGA 8 cut(s) 68, 104, 261, 715, 818, 982, 1072, 1125
MfeI CAATTG 2 cut(s) 692, 1140
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 163, 793
Mly113I GGCGCC 1 cut(s) 1048
MlyI GAGTC 1 cut(s) 684
MseI TTAA 5 cut(s) 300, 488, 558, 563, 587
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 459
MunI CAATTG 2 cut(s) 692, 1140
MvnI CGCG 2 cut(s) 574, 1219
MwoI GCNNNNNNNGC 2 cut(s) 392, 482
NarI GGCGCC 1 cut(s) 1048
NdeI CATATG 1 cut(s) 1030
NdeII GATC 4 cut(s) 138, 435, 532, 616
NlaIII CATG 3 cut(s) 83, 757, 1018
NlaIV GGNNCC 4 cut(s) 247, 718, 768, 1049
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 3 cut(s) 31, 68, 676
NspI RCATGY 1 cut(s) 1018
OliI CACNNNNGTG 1 cut(s) 29
PagI TCATGA 1 cut(s) 79
PciI ACATGT 1 cut(s) 1014
PciSI GCTCTTC 1 cut(s) 995
PcsI WCGNNNNNNNCGW 1 cut(s) 816
PfeI GAWTC 5 cut(s) 401, 502, 904, 939, 1163
PkrI GCNGC 5 cut(s) 394, 397, 421, 573, 949
PleI GAGTC 1 cut(s) 683
PluTI GGCGCC 1 cut(s) 1051
PpsI GAGTC 1 cut(s) 683
PpuMI RGGWCCY 1 cut(s) 716
PscI ACATGT 1 cut(s) 1014
PshAI GACNNNNGTC 1 cut(s) 38
PshBI ATTAAT 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 716
PspN4I GGNNCC 4 cut(s) 247, 718, 768, 1049
PspPI GGNCC 2 cut(s) 543, 716
PspPPI RGGWCCY 1 cut(s) 716
PstI CTGCAG 1 cut(s) 429
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 995
SaqAI TTAA 5 cut(s) 300, 488, 558, 563, 587
SatI GCNGC 5 cut(s) 393, 396, 420, 572, 948
Sau3AI GATC 4 cut(s) 138, 435, 532, 616
Sau96I GGNCC 2 cut(s) 543, 716
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 1 cut(s) 684
SduI GDGCHC 2 cut(s) 163, 793
SfaNI GCATC 4 cut(s) 453, 485, 588, 1118
SfcI CTRYAG 2 cut(s) 425, 513
SfoI GGCGCC 1 cut(s) 1049
SinI GGWCC 1 cut(s) 716
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 1232
SmoI CTYRAG 1 cut(s) 1232
SsiI CCGC 3 cut(s) 398, 572, 1219
SspDI GGCGCC 1 cut(s) 1047
SspMI CTAG 1 cut(s) 936
TaaI ACNGT 4 cut(s) 31, 517, 871, 1159
TaiI ACGT 1 cut(s) 52
TaqI TCGA 4 cut(s) 46, 178, 438, 505
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 574
TfiI GAWTC 5 cut(s) 401, 502, 904, 939, 1163
Tru1I TTAA 5 cut(s) 300, 488, 558, 563, 587
Tru9I TTAA 5 cut(s) 300, 488, 558, 563, 587
TscAI CASTG 3 cut(s) 36, 228, 313
TseFI GTSAC 3 cut(s) 31, 68, 676
TseI GCWGC 4 cut(s) 392, 395, 419, 947
Tsp45I GTSAC 3 cut(s) 31, 68, 676
TspDTI ATGAA 5 cut(s) 68, 105, 665, 697, 938
TspGWI ACGGA 2 cut(s) 347, 672
TspRI CASTG 3 cut(s) 36, 228, 313
VneI GTGCAC 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 716
VspI ATTAAT 1 cut(s) 300
XapI RAATTY 2 cut(s) 115, 1248
XbaI TCTAGA 1 cut(s) 935
XceI RCATGY 1 cut(s) 1018
XmiI GTMKAC 1 cut(s) 10
XspI CTAG 1 cut(s) 936
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.