Rw1G025170

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007144
Physical Location & Seq
Reverse (-)
51786335 .. 51790131
3797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025170.1

Sequence Viewer

Length: 1284 bp
ATGGCGTGTAGACGTGCTGTCTCCACCACGGCGACCCTTGCCACGAGACGTTTCCATCCTTCATTGAGTCACGTCCTTCATGATAACAATGAGGATAATAAATCACATTCACCAAGTTCAAGCACTCAACCCAACATAAAGAGTATTTTGCCAAGTGGATCTTACAACTCGACAGTTGGATTTAGCTTTGGTTCTCAAAGTAGAGGGAGGACAGGCTCATCATTGGCTTGGAAAATGGGCCTTGGATCCTTTCATTATAGGCATATGTCAACCACAATTGGTGATGGGTCTGATAAGATGGATGACATTGGATATGTTGCGGAGGTTATTACAGATAAGACTGTGGAAGTAGTTACCTTCCAAGGACCGGCTGTGAGTGAAGTGGCTGTTGCTGCAGCAGATTCTTTCTTGCCTGTTGCTGGCCTACAATACTTGATCGATTTCGTTCATTCTCTTACCGGATTGAATTGGTGGGCATCAATAGCTTTAACAACTATTTTGATTCGAGTGGCTACAATTCCTCTTCTTATTGATCAACTCAGGGCTACTACAAAGCTCAATTTGATGAGGCCTCGTTTAGAGCAGTTGAAACAAGAGATGCAAAATATGGAAGCAGATCCTGAGGTTTTAAGAGAAGGTCAGAAACGGATGATGGCATTGTTCCATGAATACGGAGTCACTCCATTTTCTCAATTGAAAGGATTATTCATTCAAGGTCCTATTTTCATCAGCTTCTTTCTTGCTATTAGAAACATGGCTGAGAAAGTTCCGTCTTTTCAAAATGGTGGAGCTCTCTGGTTTACTGATCTCACAACTCCAGATAGCATGCTGATCCTTCCAATTCTGACAGCAATGACATTCTGGATCACAGTTGAGTGCAACATGCAGGAAGGTCTGGAAGGAAATCCTGTTGCCCAAACTATGAAAACCTATTCTAGAATCCTAGCTGTCATTGCAGTTCCAGTTATGATGAGCTTCCCCAAGGCACTGTTCTGTTATTGGCTCACCTCCAACATCTTCTCTCTTACATATGGATTAGCAATTAGGCGTCCAGAGGTGAAGAGTTTACTTAATCTTCCTGAAATTCCTGAGACACCTACTGCATCACAGCCTCCATTTTCTTTGTTTGAAGCAGTTAAAAAAGACACTACTATTGAATCCGAACCTTCATTACGCACAGAAACATCGAAGGCTTCTGACAGAAGCATATCGTCGTCTTCAATTCTCAAGCAGAGACTCAAAAGTCTAGAGAAGCATGTCAAGGGGAAAAGGAAACAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
60KD_IMP

Protein Analysis

427

Amino Acids

47.36

Weight (kDa)

9.1

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 157 - 349 2.8e-29 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1242
AccI GTMKAC 1 cut(s) 10
AciI CCGC 1 cut(s) 320
AclWI GGATC 6 cut(s) 166, 240, 253, 611, 826, 872
AcsI RAATTY 1 cut(s) 1083
AcyI GRCGYC 1 cut(s) 1048
AfiI CCNNNNNNNGG 2 cut(s) 367, 419
AgsI TTSAA 9 cut(s) 120, 466, 589, 697, 713, 779, 1130, 1157, 1221
AhdI GACNNNNNGTC 1 cut(s) 17
AjiI CACGTC 2 cut(s) 14, 73
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AluBI AGCT 7 cut(s) 186, 485, 556, 732, 791, 947, 975
AluI AGCT 7 cut(s) 186, 485, 556, 732, 791, 947, 975
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 4 cut(s) 25, 40, 1085, 1228
AlwI GGATC 6 cut(s) 166, 240, 253, 611, 826, 872
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 3 cut(s) 238, 421, 569
ApeKI GCWGC 2 cut(s) 392, 395
ApoI RAATTY 1 cut(s) 1083
AspS9I GGNCC 3 cut(s) 238, 365, 716
AsuHPI GGTGA 4 cut(s) 102, 293, 997, 1069
AvaII GGWCC 2 cut(s) 365, 716
AxyI CCTNAGG 1 cut(s) 621
BamHI GGATCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 793
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 1209
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 2 cut(s) 379, 407
BccI CCATC 4 cut(s) 63, 278, 292, 646
BceAI ACGGC 1 cut(s) 45
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 4 cut(s) 25, 40, 1085, 1228
BfaI CTAG 3 cut(s) 936, 944, 1247
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 393, 396
BlsI GCNGC 2 cut(s) 394, 397
Bme18I GGWCC 2 cut(s) 365, 716
BmeRI GACNNNNNGTC 1 cut(s) 17
BmgBI CACGTC 2 cut(s) 14, 73
BmgT120I GGNCC 3 cut(s) 238, 365, 716
BmiI GGNNCC 1 cut(s) 247
BmsI GCATC 3 cut(s) 485, 588, 1112
BpiI GAAGAC 1 cut(s) 1209
BpmI CTGGAG 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 1211
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 1048
BsaJI CCNNGG 4 cut(s) 27, 241, 361, 981
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 419
Bse118I RCCGGY 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 962
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 858, 951
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 4 cut(s) 27, 241, 361, 981
BseGI GGATG 3 cut(s) 55, 307, 654
BseLI CCNNNNNNNGG 2 cut(s) 367, 419
BseMI GCAATG 2 cut(s) 858, 951
BseMII CTCAG 4 cut(s) 553, 612, 750, 1080
BseNI ACTGG 1 cut(s) 962
BseXI GCAGC 2 cut(s) 379, 407
BshFI GGCC 3 cut(s) 240, 423, 571
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 2 cut(s) 368, 459
BslI CCNNNNNNNGG 2 cut(s) 367, 419
BsmAI GTCTC 4 cut(s) 25, 40, 1085, 1228
BsmBI CGTCTC 1 cut(s) 40
BsnI GGCC 3 cut(s) 240, 423, 571
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
BspACI CCGC 1 cut(s) 320
BspANI GGCC 3 cut(s) 240, 423, 571
BspCNI CTCAG 4 cut(s) 552, 613, 751, 1081
BspDI ATCGAT 1 cut(s) 438
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 6 cut(s) 166, 240, 253, 611, 826, 872
BsrDI GCAATG 2 cut(s) 858, 951
BsrFI RCCGGY 1 cut(s) 367
BsrI ACTGG 1 cut(s) 962
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 4 cut(s) 27, 241, 361, 981
BssMI GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
BssNI GRCGYC 1 cut(s) 1048
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 3 cut(s) 241, 361, 981
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 4 cut(s) 175, 343, 871, 990
Bst6I CTCTTC 2 cut(s) 528, 1055
BstACI GRCGYC 1 cut(s) 1048
BstC8I GCNNGC 2 cut(s) 421, 827
BstDEI CTNAG 4 cut(s) 539, 621, 759, 1089
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 3 cut(s) 55, 307, 654
BstKTI GATC 8 cut(s) 161, 248, 438, 535, 619, 808, 834, 867
BstMAI GTCTC 4 cut(s) 25, 40, 1085, 1228
BstMBI GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
BstMWI GCNNNNNNNGC 4 cut(s) 38, 392, 482, 953
BstNSI RCATGY 3 cut(s) 829, 886, 1259
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 379, 407
BstV2I GAAGAC 1 cut(s) 1209
BstX2I RGATCY 3 cut(s) 158, 245, 616
BstYI RGATCY 3 cut(s) 158, 245, 616
Bsu15I ATCGAT 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 621
BsuRI GGCC 3 cut(s) 240, 423, 571
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 2 cut(s) 14, 73
BtsCI GGATG 3 cut(s) 55, 307, 654
BtsIMutI CAGTG 1 cut(s) 986
Cac8I GCNNGC 2 cut(s) 421, 827
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 238, 365, 716
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 1037
CviAII CATG 6 cut(s) 80, 665, 754, 826, 883, 1256
DdeI CTNAG 4 cut(s) 539, 621, 759, 1089
DpnI GATC 8 cut(s) 160, 247, 437, 534, 618, 807, 833, 866
DpnII GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
DrdI GACNNNNNNGTC 1 cut(s) 1242
DriI GACNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 1242
Eam1104I CTCTTC 2 cut(s) 528, 1055
Eam1105I GACNNNNNGTC 1 cut(s) 17
EarI CTCTTC 2 cut(s) 528, 1055
Ecl136II GAGCTC 1 cut(s) 791
Eco130I CCWWGG 3 cut(s) 241, 361, 981
Eco147I AGGCCT 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 793
Eco47I GGWCC 2 cut(s) 365, 716
Eco53kI GAGCTC 1 cut(s) 791
Eco81I CCTNAGG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 716
EcoT14I CCWWGG 3 cut(s) 241, 361, 981
EcoT38I GRGCYC 1 cut(s) 793
ErhI CCWWGG 3 cut(s) 241, 361, 981
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 6 cut(s) 83, 668, 757, 829, 886, 1259
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FatI CATG 6 cut(s) 79, 664, 753, 825, 882, 1255
FauNDI CATATG 2 cut(s) 264, 1030
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 2 cut(s) 393, 396
FokI GGATG 3 cut(s) 42, 314, 661
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 2 cut(s) 393, 396
FspBI CTAG 3 cut(s) 936, 944, 1247
GluI GCNGC 2 cut(s) 393, 396
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 3 cut(s) 240, 423, 571
HapII CCGG 2 cut(s) 368, 459
HgaI GACGC 1 cut(s) 1037
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 6 cut(s) 83, 668, 757, 829, 886, 1259
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 7 cut(s) 67, 401, 502, 675, 939, 1157, 1236
HpaII CCGG 2 cut(s) 368, 459
HphI GGTGA 4 cut(s) 102, 293, 997, 1069
Hpy166II GTNNAC 4 cut(s) 11, 270, 801, 1067
Hpy188I TCNGA 5 cut(s) 292, 642, 846, 1162, 1198
Hpy8I GTNNAC 4 cut(s) 11, 270, 801, 1067
Hpy99I CGWCG 1 cut(s) 1216
HpyAV CCTTC 9 cut(s) 69, 86, 367, 629, 845, 884, 893, 1176, 1183
HpyCH4III ACNGT 4 cut(s) 175, 343, 871, 990
HpyCH4IV ACGT 3 cut(s) 13, 49, 72
HpyCH4V TGCA 6 cut(s) 395, 601, 879, 886, 956, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 392, 482, 953
HpyF3I CTNAG 4 cut(s) 539, 621, 759, 1089
HpySE526I ACGT 3 cut(s) 13, 49, 72
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 6 cut(s) 83, 668, 757, 829, 886, 1259
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 2 cut(s) 379, 407
LweI GCATC 3 cut(s) 485, 588, 1112
MaeI CTAG 3 cut(s) 936, 944, 1247
MaeII ACGT 3 cut(s) 13, 49, 72
MaeIII GTNAC 3 cut(s) 68, 352, 676
MalI GATC 8 cut(s) 160, 247, 437, 534, 618, 807, 833, 866
MboI GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
MboII GAAGA 5 cut(s) 515, 1009, 1067, 1072, 1209
MfeI CAATTG 2 cut(s) 276, 692
MflI RGATCY 3 cut(s) 158, 245, 616
MhlI GDGCHC 1 cut(s) 793
MlyI GAGTC 3 cut(s) 76, 684, 1230
MmeI TCCRAC 2 cut(s) 157, 1035
MseI TTAA 4 cut(s) 488, 629, 1071, 1137
MspI CCGG 2 cut(s) 368, 459
MunI CAATTG 2 cut(s) 276, 692
MwoI GCNNNNNNNGC 4 cut(s) 38, 392, 482, 953
NdeI CATATG 2 cut(s) 264, 1030
NdeII GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
NlaIII CATG 6 cut(s) 83, 668, 757, 829, 886, 1259
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 68, 676
NspI RCATGY 3 cut(s) 829, 886, 1259
PaeI GCATGC 1 cut(s) 829
PagI TCATGA 1 cut(s) 79
PceI AGGCCT 1 cut(s) 571
PfeI GAWTC 4 cut(s) 401, 502, 939, 1157
PkrI GCNGC 2 cut(s) 394, 397
PleI GAGTC 3 cut(s) 75, 683, 1230
PpsI GAGTC 3 cut(s) 75, 683, 1230
PpuMI RGGWCCY 1 cut(s) 716
Psp124BI GAGCTC 1 cut(s) 793
Psp5II RGGWCCY 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 3 cut(s) 238, 365, 716
PspPPI RGGWCCY 1 cut(s) 716
PsrI GAACNNNNNNTAC 2 cut(s) 1155, 1187
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 158, 245, 616
SacI GAGCTC 1 cut(s) 793
SaqAI TTAA 4 cut(s) 488, 629, 1071, 1137
SatI GCNGC 2 cut(s) 393, 396
Sau3AI GATC 8 cut(s) 158, 245, 435, 532, 616, 805, 831, 864
Sau96I GGNCC 3 cut(s) 238, 365, 716
SchI GAGTC 3 cut(s) 76, 684, 1230
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 3 cut(s) 485, 588, 1112
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 2 cut(s) 365, 716
SmlI CTYRAG 1 cut(s) 1226
SmoI CTYRAG 1 cut(s) 1226
SphI GCATGC 1 cut(s) 829
SseBI AGGCCT 1 cut(s) 571
SsiI CCGC 1 cut(s) 320
SspMI CTAG 3 cut(s) 936, 944, 1247
SstI GAGCTC 1 cut(s) 793
StuI AGGCCT 1 cut(s) 571
StyI CCWWGG 3 cut(s) 241, 361, 981
TaaI ACNGT 4 cut(s) 175, 343, 871, 990
TaiI ACGT 3 cut(s) 16, 52, 75
TaqI TCGA 4 cut(s) 170, 438, 505, 1187
TfiI GAWTC 4 cut(s) 401, 502, 939, 1157
Tru1I TTAA 4 cut(s) 488, 629, 1071, 1137
Tru9I TTAA 4 cut(s) 488, 629, 1071, 1137
TscAI CASTG 1 cut(s) 993
TseFI GTSAC 2 cut(s) 68, 676
TseI GCWGC 2 cut(s) 392, 395
Tsp45I GTSAC 2 cut(s) 68, 676
TspDTI ATGAA 9 cut(s) 51, 68, 242, 437, 681, 697, 715, 938, 1158
TspGWI ACGGA 3 cut(s) 661, 687, 759
TspRI CASTG 1 cut(s) 993
VpaK11BI GGWCC 2 cut(s) 365, 716
XapI RAATTY 1 cut(s) 1083
XbaI TCTAGA 2 cut(s) 935, 1246
XceI RCATGY 3 cut(s) 829, 886, 1259
XmiI GTMKAC 1 cut(s) 10
XspI CTAG 3 cut(s) 936, 944, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.