MD07G1192800.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002230
Physical Location & Seq
Reverse (-)
27288659 .. 27293691
5033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1192800.v1.1.491

Sequence Viewer

Length: 1830 bp
ATGTCTTTCCAACCCAAAAGTTCTTGGACTCCGAGAGATTCGAGCTGTCTAACTGATGGAGAGATGGGTTATGACAATTCTTCCAGATTAGATTCAAAGCGTGGTCACCCGTGGTTTATGGATTCCAATGGGCCGGAGCTCTTCAACAACAAGAAGCAAACAATGGAGTCTGTTAATGGCAGACCAGTTTCAGAGATGGGTTATGACAATTCTTCCAGATTAGATTCAAAGCGAGGTTACCCGTGGTTTGTGGATTCCAATGGGACGGAGCTTTTCAACAACAAGAAGCAAGCAATGGATGCTTTTAATGGCAGGCTAGTTTCAGGAGTTCCACATTTGGAAAATTTCCCGTGGGAAAACACTTCAGGGTTTCAGTCAGTTCCAGGTCAATTCACTGATCGGCTTTTTGGAACTGAGCCAGTACGGACAATCAACCCAGGTGAGAGGAACATTCAATCCGTTGGAAATGGAAATATGAATTTGGGAAGAAAGGGTTTTGAGAATCAATATGGAAATGATTCATCAATGGGTTTGTCAATGTCTCACTCTATCGAAGACCCCTCATCATGTCTCAGTTTTGGTGGAATCAGAAAAGTGAAGGTCAATGAAGTTAGGGACTCTGAGAATGTAGTGTCTGCGTCTATGGGGCACTCCTACATTAGAGGAGACAACACTACAATTTCAGCAGGTACCGCGTTTAATAAGAGCAATGAAAACAGCATATCTTTGGGTTCGACTTATAACAATGGCGAAGAGAATGCAATCGCAATAGGGCCTTCCTTTAATAAGGCAGATGAAAATTTCATTTTAATGGGTCACACCTACAGTAAGGGAAATGGGAATTTCACATTGATGGCTCATAATTATAACAAGGGTGACAATGGTAACTTATCAATTGGACGGCCCTTTGACAAGGAGGACGGAAGATTTATATCAATAGGCCAGTCATATGAAAAGGGAGATAGCAGTTTCATATCGTTAGGTAGCTCTTACAACAAAGGTCATGAGAGTTTTGTTTCTAGTGGTGCAACATATAGTAAAGCAAATGAGAATTTCATCTCAATGCCTCCGACATATAACAAGGCAGATTCAAATGCTGTACCTCCCTATGATAAAGGAGATAGCAGTTCTATGAGTCATAATTACAGTAAGAGTGAGAGCACCACCATCGCTTTTGGAAGTTTTCATGATGAACTCGACACAAATCCTTCTGGCAGGATCATTAACAGCTATGATTTGCTGACGAGTAGCCAGAACATAGCTCAACAGTCAGAAGAACCTGGCCTGAAGGATCCAGTTCAGTCACATACGGATCCAAATGTAGGTGAAGCTCTGCAAGTTGATTCTAAAACTGATATGGTTCCAAAAATTAAAGAGCCAAGAACTGCTAGGAAAGCTCCTCCAAACAACTTCCCTTCAAATGTCAAAAGTTTGTTATCAACTGGTATGTTTGATGGTGTTCCAGTGAAGTATGTTTCCTGGTCACGGGAGAAAAACCTCAAGGCAACAATAAAAGGGGCCGGGTATCTTTGTTCCTGTGATCAATGCAATCACCCAAAGTCTCTTAATGCTTATGAGTTTGAGCGTCATGCTGGTGCCAAGACCAAACATCCCAATAATCACATCTACTTTGAGAATGGAAAGACCATTTATGCAGTAGTTCAAGAGCTGAAGAACACTCCTCAGGACGTGCTGTTTGACGCCATTCAAACTGTAACAGGGTCTCCTATTAATCAGAAGAATTTCCGCATCTGGAAAGCATCATATCAAGCCGCAACCCGTGAACTTCAGCGTATCTATGGAGAAGATGAAGCCGCTGTACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

610

Amino Acids

67.07

Weight (kDa)

6.1

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 503 - 556 3.9e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 741, 867
Acc36I ACCTGC 1 cut(s) 677
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 2 cut(s) 689, 1595
AccII CGCG 1 cut(s) 695
AciI CCGC 4 cut(s) 693, 1747, 1773, 1815
AclWI GGATC 5 cut(s) 1226, 1286, 1299, 1307, 1320
AcsI RAATTY 6 cut(s) 343, 478, 799, 841, 1051, 1741
AcuI CTGAAG 4 cut(s) 348, 1307, 1691, 1772
AcyI GRCGYC 1 cut(s) 1701
AfaI GTAC 4 cut(s) 423, 691, 1101, 1821
AfiI CCNNNNNNNGG 2 cut(s) 1322, 1485
AgsI TTSAA 9 cut(s) 96, 145, 228, 277, 455, 1092, 1419, 1664, 1709
AjiI CACGTC 1 cut(s) 1690
AjnI CCWGG 4 cut(s) 382, 436, 1279, 1478
AloI GAACNNNNNNTCC 2 cut(s) 440, 472
AluBI AGCT 9 cut(s) 45, 139, 271, 987, 1230, 1262, 1331, 1397, 1669
AluI AGCT 9 cut(s) 45, 139, 271, 987, 1230, 1262, 1331, 1397, 1669
Alw21I GWGCWC 2 cut(s) 141, 1163
Alw26I GTCTC 5 cut(s) 546, 575, 660, 1566, 1728
AlwI GGATC 5 cut(s) 1226, 1286, 1299, 1307, 1320
AoxI GGCC 6 cut(s) 131, 773, 902, 940, 1282, 1518
ApoI RAATTY 6 cut(s) 343, 478, 799, 841, 1051, 1741
ArsI GACNNNNNNTTYG 2 cut(s) 1679, 1711
AseI ATTAAT 1 cut(s) 1731
Asp700I GAANNNNTTC 2 cut(s) 517, 1742
Asp718I GGTACC 1 cut(s) 689
AspS9I GGNCC 4 cut(s) 131, 773, 903, 1518
AsuC2I CCSGG 1 cut(s) 1522
AsuHPI GGTGA 5 cut(s) 98, 452, 887, 1337, 1544
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 1 cut(s) 651
BamHI GGATCC 2 cut(s) 1291, 1312
BanI GGYRCC 2 cut(s) 689, 1595
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 1 cut(s) 1803
BbsI GAAGAC 1 cut(s) 561
Bbv12I GWGCWC 2 cut(s) 141, 1163
BccI CCATC 6 cut(s) 50, 58, 190, 847, 1175, 1448
BceAI ACGGC 1 cut(s) 917
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 1150, 1184
BciT130I CCWGG 4 cut(s) 384, 438, 1281, 1480
BclI TGATCA 1 cut(s) 1540
BcnI CCSGG 1 cut(s) 1522
BcoDI GTCTC 5 cut(s) 546, 575, 660, 1566, 1728
BfaI CTAG 3 cut(s) 317, 1020, 1389
BfmI CTRYAG 1 cut(s) 823
BfuAI ACCTGC 1 cut(s) 677
BisI GCNGC 2 cut(s) 1773, 1815
BlsI GCNGC 2 cut(s) 1774, 1816
Bme1390I CCNGG 5 cut(s) 384, 438, 1281, 1480, 1522
BmgBI CACGTC 1 cut(s) 1690
BmgT120I GGNCC 4 cut(s) 131, 773, 903, 1518
BmiI GGNNCC 6 cut(s) 691, 1293, 1314, 1362, 1519, 1597
BmrFI CCNGG 5 cut(s) 384, 438, 1281, 1480, 1522
BmsI GCATC 3 cut(s) 289, 1758, 1769
BpiI GAAGAC 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 1484
BpuMI CCSGG 1 cut(s) 1522
BsaBI GATNNNNATC 1 cut(s) 931
BsaHI GRCGYC 1 cut(s) 1701
BsaI GGTCTC 1 cut(s) 1728
BsaJI CCNNGG 4 cut(s) 110, 242, 350, 436
BsaXI ACNNNNNCTCC 2 cut(s) 1708, 1738
Bsc4I CCNNNNNNNGG 2 cut(s) 1322, 1485
Bse1I ACTGG 6 cut(s) 185, 419, 943, 1295, 1447, 1463
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 2 cut(s) 300, 715
Bse8I GATNNNNATC 1 cut(s) 931
BseBI CCWGG 4 cut(s) 384, 438, 1281, 1480
BseDI CCNNGG 4 cut(s) 110, 242, 350, 436
BseGI GGATG 2 cut(s) 304, 1609
BseJI GATNNNNATC 1 cut(s) 931
BseLI CCNNNNNNNGG 2 cut(s) 1322, 1485
BseMI GCAATG 2 cut(s) 300, 715
BseMII CTCAG 4 cut(s) 405, 586, 612, 1697
BseNI ACTGG 6 cut(s) 185, 419, 943, 1295, 1447, 1463
BseRI GAGGAG 3 cut(s) 678, 1389, 1671
BseSI GKGCMC 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 695
BshFI GGCC 6 cut(s) 133, 775, 904, 942, 1284, 1520
BshNI GGYRCC 2 cut(s) 689, 1595
BsiHKAI GWGCWC 2 cut(s) 141, 1163
BsiSI CCGG 2 cut(s) 134, 1521
BslFI GGGAC 2 cut(s) 277, 629
BslI CCNNNNNNNGG 2 cut(s) 1322, 1485
BsmAI GTCTC 5 cut(s) 546, 575, 660, 1566, 1728
BsmFI GGGAC 2 cut(s) 277, 629
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 6 cut(s) 133, 775, 904, 942, 1284, 1520
Bso31I GGTCTC 1 cut(s) 1728
Bsp1286I GDGCHC 3 cut(s) 141, 651, 1163
Bsp143I GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
BspACI CCGC 4 cut(s) 693, 1747, 1773, 1815
BspANI GGCC 6 cut(s) 133, 775, 904, 942, 1284, 1520
BspCNI CTCAG 4 cut(s) 406, 585, 613, 1696
BspFNI CGCG 1 cut(s) 695
BspHI TCATGA 2 cut(s) 1003, 1186
BspLI GGNNCC 6 cut(s) 691, 1293, 1314, 1362, 1519, 1597
BspMI ACCTGC 1 cut(s) 677
BspPI GGATC 5 cut(s) 1226, 1286, 1299, 1307, 1320
BspQI GCTCTTC 1 cut(s) 146
BspT107I GGYRCC 2 cut(s) 689, 1595
BspTNI GGTCTC 1 cut(s) 1728
BsrDI GCAATG 2 cut(s) 300, 715
BsrI ACTGG 6 cut(s) 185, 419, 943, 1295, 1447, 1463
BssECI CCNNGG 4 cut(s) 110, 242, 350, 436
BssMI GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
BssNI GRCGYC 1 cut(s) 1701
Bst2UI CCWGG 4 cut(s) 384, 438, 1281, 1480
Bst4CI ACNGT 4 cut(s) 827, 1148, 1269, 1714
Bst6I CTCTTC 2 cut(s) 146, 747
BstACI GRCGYC 1 cut(s) 1701
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 291, 314
BstDEI CTNAG 4 cut(s) 414, 572, 621, 1683
BstDSI CCRYGG 3 cut(s) 110, 242, 350
BstEII GGTNACC 2 cut(s) 104, 236
BstF5I GGATG 2 cut(s) 304, 1609
BstFNI CGCG 1 cut(s) 695
BstKTI GATC 5 cut(s) 400, 1221, 1294, 1315, 1543
BstMAI GTCTC 5 cut(s) 546, 575, 660, 1566, 1728
BstMBI GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
BstMWI GCNNNNNNNGC 3 cut(s) 299, 692, 1394
BstNI CCWGG 4 cut(s) 384, 438, 1281, 1480
BstPI GGTNACC 2 cut(s) 104, 236
BstSCI CCNGG 5 cut(s) 382, 436, 1279, 1478, 1520
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 1 cut(s) 651
BstUI CGCG 1 cut(s) 695
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 1291, 1312
BstYI RGATCY 2 cut(s) 1291, 1312
Bsu36I CCTNAGG 1 cut(s) 1683
BsuRI GGCC 6 cut(s) 133, 775, 904, 942, 1284, 1520
BtgI CCRYGG 3 cut(s) 110, 242, 350
BtgZI GCGATG 1 cut(s) 1153
BtrI CACGTC 1 cut(s) 1690
BtsCI GGATG 2 cut(s) 304, 1609
BtsIMutI CAGTG 2 cut(s) 393, 1470
BveI ACCTGC 1 cut(s) 677
Cac8I GCNNGC 2 cut(s) 291, 314
CciI TCATGA 2 cut(s) 1003, 1186
Cfr13I GGNCC 4 cut(s) 131, 773, 903, 1518
CseI GACGC 3 cut(s) 627, 1574, 1709
Csp6I GTAC 4 cut(s) 422, 690, 1100, 1820
CviAII CATG 4 cut(s) 567, 1004, 1187, 1589
CviQI GTAC 4 cut(s) 422, 690, 1100, 1820
DdeI CTNAG 4 cut(s) 414, 572, 621, 1683
DpnI GATC 5 cut(s) 399, 1220, 1293, 1314, 1542
DpnII GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
Eam1104I CTCTTC 2 cut(s) 146, 747
EarI CTCTTC 2 cut(s) 146, 747
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 1728
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 4 cut(s) 348, 1307, 1691, 1772
Eco81I CCTNAGG 1 cut(s) 1683
Eco91I GGTNACC 2 cut(s) 104, 236
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 773
EcoO65I GGTNACC 2 cut(s) 104, 236
EcoRII CCWGG 4 cut(s) 382, 436, 1279, 1478
EcoT38I GRGCYC 1 cut(s) 141
FaeI CATG 4 cut(s) 570, 1007, 1190, 1592
FaqI GGGAC 2 cut(s) 277, 629
FatI CATG 4 cut(s) 566, 1003, 1186, 1588
FauNDI CATATG 1 cut(s) 949
FbaI TGATCA 1 cut(s) 1540
Fnu4HI GCNGC 2 cut(s) 1773, 1815
FokI GGATG 2 cut(s) 311, 1596
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 1773, 1815
FspBI CTAG 3 cut(s) 317, 1020, 1389
GluI GCNGC 2 cut(s) 1773, 1815
HaeIII GGCC 6 cut(s) 133, 775, 904, 942, 1284, 1520
HapII CCGG 2 cut(s) 134, 1521
HgaI GACGC 3 cut(s) 627, 1574, 1709
Hin1I GRCGYC 1 cut(s) 1701
Hin1II CATG 4 cut(s) 570, 1007, 1190, 1592
HpaII CCGG 2 cut(s) 134, 1521
HphI GGTGA 5 cut(s) 98, 452, 887, 1337, 1544
Hpy166II GTNNAC 1 cut(s) 1784
Hpy188I TCNGA 7 cut(s) 33, 193, 590, 622, 1071, 1273, 1737
Hpy188III TCNNGA 9 cut(s) 84, 216, 324, 1004, 1187, 1664, 1685, 1753, 1827
Hpy8I GTNNAC 1 cut(s) 1784
HpyAV CCTTC 5 cut(s) 592, 786, 1218, 1282, 1425
HpyCH4III ACNGT 4 cut(s) 827, 1148, 1269, 1714
HpyCH4IV ACGT 1 cut(s) 1689
HpyCH4V TGCA 5 cut(s) 761, 1028, 1336, 1548, 1655
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 692, 1394
HpyF3I CTNAG 4 cut(s) 414, 572, 621, 1683
HpySE526I ACGT 1 cut(s) 1689
Hsp92I GRCGYC 1 cut(s) 1701
Hsp92II CATG 4 cut(s) 570, 1007, 1190, 1592
KpnI GGTACC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 1540
Kzo9I GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 3 cut(s) 136, 268, 1402
LweI GCATC 3 cut(s) 289, 1758, 1769
MaeI CTAG 3 cut(s) 317, 1020, 1389
MaeII ACGT 1 cut(s) 1689
MaeIII GTNAC 8 cut(s) 104, 236, 815, 875, 884, 1302, 1482, 1714
MalI GATC 5 cut(s) 399, 1220, 1293, 1314, 1542
MboI GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 2 cut(s) 1291, 1312
MhlI GDGCHC 3 cut(s) 141, 651, 1163
MlyI GAGTC 4 cut(s) 22, 176, 611, 1144
MmeI TCCRAC 3 cut(s) 34, 442, 1094
MroXI GAANNNNTTC 2 cut(s) 517, 1742
MseI TTAA 9 cut(s) 174, 306, 699, 783, 809, 1224, 1371, 1566, 1731
MslI CAYNNNNRTG 4 cut(s) 809, 851, 1061, 1593
MspA1I CMGCKG 1 cut(s) 1817
MspI CCGG 2 cut(s) 134, 1521
MspR9I CCNGG 5 cut(s) 384, 438, 1281, 1480, 1522
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 4 cut(s) 384, 438, 1281, 1480
MvnI CGCG 1 cut(s) 695
MwoI GCNNNNNNNGC 3 cut(s) 299, 692, 1394
NciI CCSGG 1 cut(s) 1522
NdeI CATATG 1 cut(s) 949
NdeII GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
NlaIII CATG 4 cut(s) 570, 1007, 1190, 1592
NlaIV GGNNCC 6 cut(s) 691, 1293, 1314, 1362, 1519, 1597
NmuCI GTSAC 5 cut(s) 104, 815, 875, 1302, 1482
PagI TCATGA 2 cut(s) 1003, 1186
PciSI GCTCTTC 1 cut(s) 146
PctI GAATGC 1 cut(s) 763
PdmI GAANNNNTTC 2 cut(s) 517, 1742
PkrI GCNGC 2 cut(s) 1774, 1816
PleI GAGTC 4 cut(s) 22, 175, 611, 1143
PpsI GAGTC 4 cut(s) 22, 175, 611, 1143
PshBI ATTAAT 1 cut(s) 1731
PsiI TTATAA 2 cut(s) 741, 867
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 4 cut(s) 382, 436, 1279, 1478
PspEI GGTNACC 2 cut(s) 104, 236
PspGI CCWGG 4 cut(s) 382, 436, 1279, 1478
PspN4I GGNNCC 6 cut(s) 691, 1293, 1314, 1362, 1519, 1597
PspPI GGNCC 4 cut(s) 131, 773, 903, 1518
PsuI RGATCY 2 cut(s) 1291, 1312
RsaI GTAC 4 cut(s) 423, 691, 1101, 1821
RsaNI GTAC 4 cut(s) 422, 690, 1100, 1820
RseI CAYNNNNRTG 4 cut(s) 809, 851, 1061, 1593
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 9 cut(s) 174, 306, 699, 783, 809, 1224, 1371, 1566, 1731
SatI GCNGC 2 cut(s) 1773, 1815
Sau3AI GATC 5 cut(s) 397, 1218, 1291, 1312, 1540
Sau96I GGNCC 4 cut(s) 131, 773, 903, 1518
SchI GAGTC 4 cut(s) 22, 176, 611, 1144
ScrFI CCNGG 5 cut(s) 384, 438, 1281, 1480, 1522
SduI GDGCHC 3 cut(s) 141, 651, 1163
SfaNI GCATC 3 cut(s) 289, 1758, 1769
SfcI CTRYAG 1 cut(s) 823
SmiMI CAYNNNNRTG 4 cut(s) 809, 851, 1061, 1593
SmlI CTYRAG 1 cut(s) 1499
SmoI CTYRAG 1 cut(s) 1499
SsiI CCGC 4 cut(s) 693, 1747, 1773, 1815
SspMI CTAG 3 cut(s) 317, 1020, 1389
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 5 cut(s) 382, 436, 1279, 1478, 1520
TaaI ACNGT 4 cut(s) 827, 1148, 1269, 1714
TaiI ACGT 1 cut(s) 1692
TaqI TCGA 4 cut(s) 41, 552, 734, 1197
TauI GCSGC 2 cut(s) 1775, 1817
Tru1I TTAA 9 cut(s) 174, 306, 699, 783, 809, 1224, 1371, 1566, 1731
Tru9I TTAA 9 cut(s) 174, 306, 699, 783, 809, 1224, 1371, 1566, 1731
TscAI CASTG 2 cut(s) 400, 1470
TseFI GTSAC 5 cut(s) 104, 815, 875, 1302, 1482
Tsp45I GTSAC 5 cut(s) 104, 815, 875, 1302, 1482
TspGWI ACGGA 5 cut(s) 281, 439, 448, 936, 1325
TspRI CASTG 2 cut(s) 400, 1470
VspI ATTAAT 1 cut(s) 1731
XapI RAATTY 6 cut(s) 343, 478, 799, 841, 1051, 1741
XmnI GAANNNNTTC 2 cut(s) 517, 1742
XspI CTAG 3 cut(s) 317, 1020, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.