Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002166

Physical Location & Seq

Genomic Coordinates Forward (+)

35695781 .. 35697110

Gene Structure 1330 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1296800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 381 bp

ATGCAGATCTTCGTGAAAACCCTAACAGGGAAGACTATCACCCTCGAGGTGGAGTCTAGCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGGTATTCCCCCGGATCAGCAGAGGCTCATTTTTGCCGGGAAGCAGCTTGAGGATGGCCGTACTCTCGCCGACTACAACATCCAGAAAGAATCAACTCTGCATTTGGTTCTGAGGCTTCGTGGTGGGATTATTGAGCCGTCTCTCATGGCATTGGCTAGGAAATACAACCAGGAAAAGATGATCTGCCGCAAGTGCTATGCCCGCCTGCACCCCCGTGCAGTTAACTGCAGGAAGAAGAAGTGTGGACACAGCAACCAGGTATTTCATATAGTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_L40e ubiquitin

Protein Analysis

127

Amino Acids

14.41

Weight (kDa)

9.51

Isoelectric Point (pI)

33.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad60-SLD	PF11976	1 - 71	8.5e-18	Ubiquitin-2 like Rad60 SUMO-like
ubiquitin	PF00240	3 - 74	1.1e-32	Ubiquitin family
Ribosomal_L40e	PF01020	78 - 121	1.2e-27	Ribosomal L40e family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36170 AT3G52590
fragaria_vesca	FvH4_6g19302 FvH4_6g19302 FvH4_7g32551
malus_domestica	MD01G1226400.v1.1 MD07G1296800.v1.1 MD12G1028600.v1.1 MD14G1028800.v1.1
prunus_persica	Prupe.2G316400_v2.0.a1 Prupe.7G102800_v2.0.a1
pyrus_communis	pycom01g23500 pycom07g26950 pycom12g02740 pycom14g02690
rosa_chinensis	RchiOBHm_Chr1g0336321 RchiOBHm_Chr1g0382401 RchiOBHm_Chr2g0129851 RchiOBHm_Chr2g0148571 RchiOBHm_Chr2g0162391 RchiOBHm_Chr3g0474431 RchiOBHm_Chr3g0474471 RchiOBHm_Chr3g0485451 RchiOBHm_Chr4g0402971
rosa_multiflora	Rmu_sc0000048.1_g000015 Rmu_sc0009916.1_g000009
rosa_roxburghii	Rroxscaffold_1G00002380 Rroxscaffold_4G00278130
rosa_rugosa	Rorug01G0434100 Rorug03G0140900
rosa_samantha	Rh1AG458800 Rh1BG415100 Rh1CG429200 Rh1DG447200 Rh2BG576000 Rh3AG191600 Rh3AG267800 Rh3BG221100 Rh3BG221300 Rh4CG124000
rosa_wichuraiana	Rw1G041220 Rw3G017490 Rw6G010860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	44
AciI	CCGC	2 cut(s)	289, 304
AclWI	GGATC	2 cut(s)	82, 123
AcoI	YGGCCR	1 cut(s)	157
AfaI	GTAC	1 cut(s)	163
AfiI	CCNNNNNNNGG	3 cut(s)	27, 49, 97
AjnI	CCWGG	3 cut(s)	90, 270, 357
AleI	CACNNNNGTG	1 cut(s)	315
AluBI	AGCT	1 cut(s)	148
AluI	AGCT	1 cut(s)	148
Alw26I	GTCTC	1 cut(s)	246
AlwI	GGATC	2 cut(s)	82, 123
Ama87I	CYCGRG	1 cut(s)	44
AoxI	GGCC	2 cut(s)	81, 157
ApeKI	GCWGC	1 cut(s)	145
Asp700I	GAANNNNTTC	1 cut(s)	105
AsuC2I	CCSGG	2 cut(s)	113, 139
AsuHPI	GGTGA	1 cut(s)	31
AvaI	CYCGRG	1 cut(s)	44
BbsI	GAAGAC	1 cut(s)	38
BbvI	GCAGC	1 cut(s)	157
BccI	CCATC	2 cut(s)	74, 149
BceAI	ACGGC	2 cut(s)	144, 223
BciT130I	CCWGG	3 cut(s)	92, 272, 359
BcnI	CCSGG	2 cut(s)	113, 139
BcoDI	GTCTC	1 cut(s)	246
BfaI	CTAG	2 cut(s)	57, 258
BfmI	CTRYAG	1 cut(s)	328
BglII	AGATCT	1 cut(s)	6
BisI	GCNGC	2 cut(s)	146, 289
BlsI	GCNGC	2 cut(s)	147, 290
Bme1390I	CCNGG	5 cut(s)	92, 113, 139, 272, 359
BmeT110I	CYCGRG	1 cut(s)	44
BmrFI	CCNGG	5 cut(s)	92, 113, 139, 272, 359
BpiI	GAAGAC	1 cut(s)	38
BpuEI	CTTGAG	1 cut(s)	170
BpuMI	CCSGG	2 cut(s)	113, 139
BsaJI	CCNNGG	1 cut(s)	111
Bsc4I	CCNNNNNNNGG	3 cut(s)	27, 49, 97
BseBI	CCWGG	3 cut(s)	92, 272, 359
BseDI	CCNNGG	1 cut(s)	111
BseGI	GGATG	2 cut(s)	160, 180
BseLI	CCNNNNNNNGG	3 cut(s)	27, 49, 97
BseMII	CTCAG	1 cut(s)	203
BseXI	GCAGC	1 cut(s)	157
BsgI	GTGCAG	2 cut(s)	293, 339
BshFI	GGCC	2 cut(s)	83, 159
BsiHKCI	CYCGRG	1 cut(s)	44
BsiSI	CCGG	2 cut(s)	113, 138
BslI	CCNNNNNNNGG	3 cut(s)	27, 49, 97
BsmAI	GTCTC	1 cut(s)	246
BsmBI	CGTCTC	1 cut(s)	246
BsnI	GGCC	2 cut(s)	83, 159
BsoBI	CYCGRG	1 cut(s)	44
Bsp143I	GATC	4 cut(s)	6, 87, 115, 282
BspACI	CCGC	2 cut(s)	289, 304
BspANI	GGCC	2 cut(s)	83, 159
BspCNI	CTCAG	1 cut(s)	204
BspMAI	CTGCAG	1 cut(s)	332
BspPI	GGATC	2 cut(s)	82, 123
BssECI	CCNNGG	1 cut(s)	111
BssMI	GATC	4 cut(s)	6, 87, 115, 282
Bst2UI	CCWGG	3 cut(s)	92, 272, 359
BstC8I	GCNNGC	2 cut(s)	304, 308
BstDEI	CTNAG	1 cut(s)	212
BstF5I	GGATG	2 cut(s)	160, 180
BstKTI	GATC	4 cut(s)	9, 90, 118, 285
BstMAI	GTCTC	1 cut(s)	246
BstMBI	GATC	4 cut(s)	6, 87, 115, 282
BstMWI	GCNNNNNNNGC	2 cut(s)	294, 303
BstNI	CCWGG	3 cut(s)	92, 272, 359
BstSCI	CCNGG	5 cut(s)	90, 111, 137, 270, 357
BstSFI	CTRYAG	1 cut(s)	328
BstV1I	GCAGC	1 cut(s)	157
BstV2I	GAAGAC	1 cut(s)	38
BstX2I	RGATCY	2 cut(s)	6, 87
BstYI	RGATCY	2 cut(s)	6, 87
BsuRI	GGCC	2 cut(s)	83, 159
BtsCI	GGATG	2 cut(s)	160, 180
Cac8I	GCNNGC	2 cut(s)	304, 308
CsiI	ACCWGGT	1 cut(s)	357
Csp6I	GTAC	1 cut(s)	162
CviAII	CATG	1 cut(s)	247
CviJI	RGCY	7 cut(s)	83, 127, 148, 159, 217, 238, 257
CviKI_1	RGCY	7 cut(s)	83, 127, 148, 159, 217, 238, 257
CviQI	GTAC	1 cut(s)	162
DdeI	CTNAG	1 cut(s)	212
DpnI	GATC	4 cut(s)	8, 89, 117, 284
DpnII	GATC	4 cut(s)	6, 87, 115, 282
EaeI	YGGCCR	1 cut(s)	157
Eco88I	CYCGRG	1 cut(s)	44
EcoRII	CCWGG	3 cut(s)	90, 270, 357
Esp3I	CGTCTC	1 cut(s)	246
FaeI	CATG	1 cut(s)	250
FaiI	YATR	4 cut(s)	248, 300, 369, 371
FatI	CATG	1 cut(s)	246
FauI	CCCGC	1 cut(s)	311
Fnu4HI	GCNGC	2 cut(s)	146, 289
FokI	GGATG	2 cut(s)	167, 167
Fsp4HI	GCNGC	2 cut(s)	146, 289
FspBI	CTAG	2 cut(s)	57, 258
GluI	GCNGC	2 cut(s)	146, 289
HaeIII	GGCC	2 cut(s)	83, 159
HapII	CCGG	2 cut(s)	113, 138
Hin1II	CATG	1 cut(s)	250
HincII	GTYRAC	1 cut(s)	325
HindII	GTYRAC	1 cut(s)	325
HinfI	GANTC	2 cut(s)	53, 191
HpaI	GTTAAC	1 cut(s)	325
HpaII	CCGG	2 cut(s)	113, 138
HphI	GGTGA	1 cut(s)	31
Hpy166II	GTNNAC	2 cut(s)	325, 347
Hpy188I	TCNGA	1 cut(s)	213
Hpy188III	TCNNGA	2 cut(s)	13, 184
Hpy8I	GTNNAC	2 cut(s)	325, 347
HpyAV	CCTTC	1 cut(s)	95
HpyCH4IV	ACGT	1 cut(s)	75
HpyCH4V	TGCA	5 cut(s)	4, 202, 310, 320, 330
HpyF10VI	GCNNNNNNNGC	2 cut(s)	294, 303
HpyF3I	CTNAG	1 cut(s)	212
HpySE526I	ACGT	1 cut(s)	75
Hsp92II	CATG	1 cut(s)	250
KspAI	GTTAAC	1 cut(s)	325
Kzo9I	GATC	4 cut(s)	6, 87, 115, 282
Lsp1109I	GCAGC	1 cut(s)	157
MabI	ACCWGGT	1 cut(s)	357
MaeI	CTAG	2 cut(s)	57, 258
MaeII	ACGT	1 cut(s)	75
MalI	GATC	4 cut(s)	8, 89, 117, 284
MboI	GATC	4 cut(s)	6, 87, 115, 282
MboII	GAAGA	3 cut(s)	43, 346, 349
MflI	RGATCY	2 cut(s)	6, 87
MlyI	GAGTC	1 cut(s)	62
MnlI	CCTC	5 cut(s)	40, 53, 117, 145, 207
MroXI	GAANNNNTTC	1 cut(s)	105
MseI	TTAA	1 cut(s)	324
MslI	CAYNNNNRTG	1 cut(s)	315
MspI	CCGG	2 cut(s)	113, 138
MspR9I	CCNGG	5 cut(s)	92, 113, 139, 272, 359
MvaI	CCWGG	3 cut(s)	92, 272, 359
MwoI	GCNNNNNNNGC	2 cut(s)	294, 303
NciI	CCSGG	2 cut(s)	113, 139
NdeII	GATC	4 cut(s)	6, 87, 115, 282
NlaIII	CATG	1 cut(s)	250
OliI	CACNNNNGTG	1 cut(s)	315
PaeR7I	CTCGAG	1 cut(s)	44
PdmI	GAANNNNTTC	1 cut(s)	105
PfeI	GAWTC	1 cut(s)	191
PflFI	GACNNNGTC	1 cut(s)	74
PfoI	TCCNGGA	1 cut(s)	90
PkrI	GCNGC	2 cut(s)	147, 290
PleI	GAGTC	1 cut(s)	61
PpsI	GAGTC	1 cut(s)	61
Psp6I	CCWGG	3 cut(s)	90, 270, 357
PspGI	CCWGG	3 cut(s)	90, 270, 357
PspXI	VCTCGAGB	1 cut(s)	44
PstI	CTGCAG	1 cut(s)	332
PsuI	RGATCY	2 cut(s)	6, 87
PsyI	GACNNNGTC	1 cut(s)	74
RsaI	GTAC	1 cut(s)	163
RsaNI	GTAC	1 cut(s)	162
RseI	CAYNNNNRTG	1 cut(s)	315
SaqAI	TTAA	1 cut(s)	324
SatI	GCNGC	2 cut(s)	146, 289
Sau3AI	GATC	4 cut(s)	6, 87, 115, 282
SchI	GAGTC	1 cut(s)	62
ScrFI	CCNGG	5 cut(s)	92, 113, 139, 272, 359
SetI	ASST	5 cut(s)	51, 78, 106, 150, 363
SexAI	ACCWGGT	1 cut(s)	357
SfcI	CTRYAG	1 cut(s)	328
Sfr274I	CTCGAG	1 cut(s)	44
SlaI	CTCGAG	1 cut(s)	44
SmiMI	CAYNNNNRTG	1 cut(s)	315
SmlI	CTYRAG	2 cut(s)	44, 149
SmoI	CTYRAG	2 cut(s)	44, 149
SsiI	CCGC	2 cut(s)	289, 304
SspMI	CTAG	2 cut(s)	57, 258
StyD4I	CCNGG	5 cut(s)	90, 111, 137, 270, 357
TaiI	ACGT	1 cut(s)	78
TaqI	TCGA	2 cut(s)	45, 69
TauI	GCSGC	1 cut(s)	291
TfiI	GAWTC	1 cut(s)	191
Tru1I	TTAA	1 cut(s)	324
Tru9I	TTAA	1 cut(s)	324
TseI	GCWGC	1 cut(s)	145
TspDTI	ATGAA	1 cut(s)	356
Tth111I	GACNNNGTC	1 cut(s)	74
XhoI	CTCGAG	1 cut(s)	44
XmnI	GAANNNNTTC	1 cut(s)	105
XspI	CTAG	2 cut(s)	57, 258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.