Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002166

Physical Location & Seq

Genomic Coordinates Reverse (-)

3184873 .. 3187091

Gene Structure 2219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1028600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 387 bp

ATGCAGATTTTCGTGAAAACCCTAACCGGGAAGACCATCACTCTCGAGGTCGAGAGCAGCGACACTATAGACAATGTCAAGGCCAAGATCCAGGACAAGGAGGGCATTCCCCCGGACCAGCAGCGCCTGATCTTCGCCGGCAAGCAGCTCGAAGACGGCCGAACCCTAGCTGACTACAACATCCAGAAAGAGTCGACTCTTCATCTGGTGCTGAGGCTCCGGGGAGGGATTATCGAGCCTTCTCTGATGGCTTTGGCTCGCAAGTACAATCAGGAGAAGATGATTTGCAGAAAGTGCTATGCTCGTCTGCATCCAAGAGCAGTGAACTGTAGGAAGAAGAAGTGTGGCCACAGCAATCAGTTGAGGCCCAAGAAGAAGATCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03010 ko03320 map03010 map03320

Pfam Domains

Protein Families

Ribosomal_L40e ubiquitin

Protein Analysis

129

Amino Acids

14.69

Weight (kDa)

9.94

Isoelectric Point (pI)

33.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad60-SLD	PF11976	1 - 71	8.9e-18	Ubiquitin-2 like Rad60 SUMO-like
ubiquitin	PF00240	3 - 74	1.1e-32	Ubiquitin family
Ribosomal_L40e	PF01020	78 - 127	3.7e-31	Ribosomal L40e family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36170 AT3G52590
fragaria_vesca	FvH4_6g19302 FvH4_6g19302 FvH4_7g32551
malus_domestica	MD01G1226400.v1.1 MD07G1296800.v1.1 MD12G1028600.v1.1 MD14G1028800.v1.1
prunus_persica	Prupe.2G316400_v2.0.a1 Prupe.7G102800_v2.0.a1
pyrus_communis	pycom01g23500 pycom07g26950 pycom12g02740 pycom14g02690
rosa_chinensis	RchiOBHm_Chr1g0336321 RchiOBHm_Chr1g0382401 RchiOBHm_Chr2g0129851 RchiOBHm_Chr2g0148571 RchiOBHm_Chr2g0162391 RchiOBHm_Chr3g0474431 RchiOBHm_Chr3g0474471 RchiOBHm_Chr3g0485451 RchiOBHm_Chr4g0402971
rosa_multiflora	Rmu_sc0000048.1_g000015 Rmu_sc0009916.1_g000009
rosa_roxburghii	Rroxscaffold_1G00002380 Rroxscaffold_4G00278130
rosa_rugosa	Rorug01G0434100 Rorug03G0140900
rosa_samantha	Rh1AG458800 Rh1BG415100 Rh1CG429200 Rh1DG447200 Rh2BG576000 Rh3AG191600 Rh3AG267800 Rh3BG221100 Rh3BG221300 Rh4CG124000
rosa_wichuraiana	Rw1G041220 Rw3G017490 Rw6G010860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	194
AclWI	GGATC	1 cut(s)	82
AcoI	YGGCCR	2 cut(s)	157, 346
AfaI	GTAC	1 cut(s)	266
AfiI	CCNNNNNNNGG	2 cut(s)	27, 97
AjnI	CCWGG	1 cut(s)	90
AluBI	AGCT	2 cut(s)	148, 170
AluI	AGCT	2 cut(s)	148, 170
AlwI	GGATC	1 cut(s)	82
AlwNI	CAGNNNCTG	1 cut(s)	127
Ama87I	CYCGRG	1 cut(s)	44
AoxI	GGCC	4 cut(s)	81, 157, 346, 365
ApeKI	GCWGC	3 cut(s)	57, 121, 145
AspLEI	GCGC	1 cut(s)	126
AspS9I	GGNCC	2 cut(s)	115, 366
AsuC2I	CCSGG	3 cut(s)	28, 113, 221
AvaI	CYCGRG	1 cut(s)	44
AvaII	GGWCC	1 cut(s)	115
BalI	TGGCCA	1 cut(s)	348
BbsI	GAAGAC	2 cut(s)	38, 159
BbvCI	CCTCAGC	1 cut(s)	212
BbvI	GCAGC	3 cut(s)	69, 133, 157
BccI	CCATC	2 cut(s)	44, 241
BceAI	ACGGC	1 cut(s)	172
BcgI	CGANNNNNNTGC	2 cut(s)	130, 164
BciT130I	CCWGG	1 cut(s)	92
BcnI	CCSGG	3 cut(s)	28, 113, 221
BfaI	CTAG	1 cut(s)	167
BfmI	CTRYAG	2 cut(s)	66, 328
BfoI	RGCGCY	1 cut(s)	127
BisI	GCNGC	3 cut(s)	58, 122, 146
BlsI	GCNGC	3 cut(s)	59, 123, 147
Bme1390I	CCNGG	4 cut(s)	28, 92, 113, 221
Bme18I	GGWCC	1 cut(s)	115
BmeT110I	CYCGRG	1 cut(s)	44
BmgT120I	GGNCC	2 cut(s)	115, 366
BmiI	GGNNCC	1 cut(s)	218
BmrFI	CCNGG	4 cut(s)	28, 92, 113, 221
BmsI	GCATC	1 cut(s)	319
BpiI	GAAGAC	2 cut(s)	38, 159
Bpu10I	CCTNAGC	1 cut(s)	212
BpuMI	CCSGG	3 cut(s)	28, 113, 221
BsaJI	CCNNGG	2 cut(s)	111, 220
Bsc4I	CCNNNNNNNGG	2 cut(s)	27, 97
Bse118I	RCCGGY	1 cut(s)	137
BseBI	CCWGG	1 cut(s)	92
BseDI	CCNNGG	2 cut(s)	111, 220
BseGI	GGATG	2 cut(s)	180, 310
BseLI	CCNNNNNNNGG	2 cut(s)	27, 97
BseMII	CTCAG	1 cut(s)	203
BseX3I	CGGCCG	1 cut(s)	157
BseXI	GCAGC	3 cut(s)	69, 133, 157
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	4 cut(s)	83, 159, 348, 367
BsiEI	CGRYCG	1 cut(s)	160
BsiHKCI	CYCGRG	1 cut(s)	44
BsiSI	CCGG	4 cut(s)	27, 113, 138, 220
BslI	CCNNNNNNNGG	2 cut(s)	27, 97
BsmI	GAATGC	1 cut(s)	105
BsnI	GGCC	4 cut(s)	83, 159, 348, 367
BsoBI	CYCGRG	1 cut(s)	44
Bsp143I	GATC	3 cut(s)	87, 129, 378
BspANI	GGCC	4 cut(s)	83, 159, 348, 367
BspCNI	CTCAG	1 cut(s)	204
BspLI	GGNNCC	1 cut(s)	218
BspPI	GGATC	1 cut(s)	82
BsrFI	RCCGGY	1 cut(s)	137
BssAI	RCCGGY	1 cut(s)	137
BssECI	CCNNGG	2 cut(s)	111, 220
BssMI	GATC	3 cut(s)	87, 129, 378
Bst2UI	CCWGG	1 cut(s)	92
Bst4CI	ACNGT	1 cut(s)	329
Bst6I	CTCTTC	1 cut(s)	204
BstAPI	GCANNNNNTGC	1 cut(s)	294
BstC8I	GCNNGC	3 cut(s)	139, 143, 259
BstDEI	CTNAG	1 cut(s)	212
BstF5I	GGATG	2 cut(s)	180, 310
BstH2I	RGCGCY	1 cut(s)	127
BstHHI	GCGC	1 cut(s)	126
BstKTI	GATC	3 cut(s)	90, 132, 381
BstMBI	GATC	3 cut(s)	87, 129, 378
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	1 cut(s)	294
BstNI	CCWGG	1 cut(s)	92
BstSCI	CCNGG	4 cut(s)	26, 90, 111, 219
BstSFI	CTRYAG	2 cut(s)	66, 328
BstV1I	GCAGC	3 cut(s)	69, 133, 157
BstV2I	GAAGAC	2 cut(s)	38, 159
BstX2I	RGATCY	1 cut(s)	87
BstYI	RGATCY	1 cut(s)	87
BstZI	CGGCCG	1 cut(s)	157
BsuRI	GGCC	4 cut(s)	83, 159, 348, 367
BtsCI	GGATG	2 cut(s)	180, 310
BtsI	GCAGTG	1 cut(s)	327
BtsIMutI	CAGTG	1 cut(s)	327
Cac8I	GCNNGC	3 cut(s)	139, 143, 259
CaiI	CAGNNNCTG	1 cut(s)	127
CfoI	GCGC	1 cut(s)	126
Cfr10I	RCCGGY	1 cut(s)	137
Cfr13I	GGNCC	2 cut(s)	115, 366
Csp6I	GTAC	1 cut(s)	265
CviQI	GTAC	1 cut(s)	265
DdeI	CTNAG	1 cut(s)	212
DpnI	GATC	3 cut(s)	89, 131, 380
DpnII	GATC	3 cut(s)	87, 129, 378
EaeI	YGGCCR	2 cut(s)	157, 346
EagI	CGGCCG	1 cut(s)	157
Eam1104I	CTCTTC	1 cut(s)	204
EarI	CTCTTC	1 cut(s)	204
EclXI	CGGCCG	1 cut(s)	157
Eco47I	GGWCC	1 cut(s)	115
Eco52I	CGGCCG	1 cut(s)	157
Eco88I	CYCGRG	1 cut(s)	44
EcoRII	CCWGG	1 cut(s)	90
FaiI	YATR	2 cut(s)	68, 300
FblI	GTMKAC	1 cut(s)	194
Fnu4HI	GCNGC	3 cut(s)	58, 122, 146
FokI	GGATG	2 cut(s)	167, 297
Fsp4HI	GCNGC	3 cut(s)	58, 122, 146
FspBI	CTAG	1 cut(s)	167
GlaI	GCGC	1 cut(s)	125
GluI	GCNGC	3 cut(s)	58, 122, 146
HaeII	RGCGCY	1 cut(s)	127
HaeIII	GGCC	4 cut(s)	83, 159, 348, 367
HapII	CCGG	4 cut(s)	27, 113, 138, 220
HhaI	GCGC	1 cut(s)	126
Hin6I	GCGC	1 cut(s)	124
HinP1I	GCGC	1 cut(s)	124
HincII	GTYRAC	1 cut(s)	195
HindII	GTYRAC	1 cut(s)	195
HinfI	GANTC	2 cut(s)	191, 196
HpaII	CCGG	4 cut(s)	27, 113, 138, 220
Hpy166II	GTNNAC	2 cut(s)	195, 325
Hpy188I	TCNGA	1 cut(s)	246
Hpy188III	TCNNGA	5 cut(s)	13, 44, 52, 184, 272
Hpy8I	GTNNAC	2 cut(s)	195, 325
HpyAV	CCTTC	1 cut(s)	249
HpyCH4III	ACNGT	1 cut(s)	329
HpyCH4V	TGCA	3 cut(s)	4, 288, 310
HpyF10VI	GCNNNNNNNGC	1 cut(s)	294
HpyF3I	CTNAG	1 cut(s)	212
HspAI	GCGC	1 cut(s)	124
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
Kzo9I	GATC	3 cut(s)	87, 129, 378
LmnI	GCTCC	1 cut(s)	222
Lsp1109I	GCAGC	3 cut(s)	69, 133, 157
LweI	GCATC	1 cut(s)	319
MaeI	CTAG	1 cut(s)	167
MalI	GATC	3 cut(s)	89, 131, 380
MboI	GATC	3 cut(s)	87, 129, 378
MboII	GAAGA	8 cut(s)	43, 124, 164, 191, 289, 346, 349, 385
MflI	RGATCY	1 cut(s)	87
MlsI	TGGCCA	1 cut(s)	348
MluNI	TGGCCA	1 cut(s)	348
MlyI	GAGTC	2 cut(s)	190, 200
MnlI	CCTC	5 cut(s)	40, 94, 207, 218, 357
Mox20I	TGGCCA	1 cut(s)	348
MroNI	GCCGGC	1 cut(s)	137
MscI	TGGCCA	1 cut(s)	348
Msp20I	TGGCCA	1 cut(s)	348
MspI	CCGG	4 cut(s)	27, 113, 138, 220
MspR9I	CCNGG	4 cut(s)	28, 92, 113, 221
Mva1269I	GAATGC	1 cut(s)	105
MvaI	CCWGG	1 cut(s)	92
MwoI	GCNNNNNNNGC	1 cut(s)	294
NaeI	GCCGGC	1 cut(s)	139
NciI	CCSGG	3 cut(s)	28, 113, 221
NdeII	GATC	3 cut(s)	87, 129, 378
NgoMIV	GCCGGC	1 cut(s)	137
NlaIV	GGNNCC	1 cut(s)	218
PaeR7I	CTCGAG	1 cut(s)	44
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PctI	GAATGC	1 cut(s)	105
PdiI	GCCGGC	1 cut(s)	139
PflFI	GACNNNGTC	1 cut(s)	74
PfoI	TCCNGGA	1 cut(s)	90
PkrI	GCNGC	3 cut(s)	59, 123, 147
PleI	GAGTC	2 cut(s)	190, 199
PpsI	GAGTC	2 cut(s)	190, 199
Psp6I	CCWGG	1 cut(s)	90
PspGI	CCWGG	1 cut(s)	90
PspN4I	GGNNCC	1 cut(s)	218
PspPI	GGNCC	2 cut(s)	115, 366
PstNI	CAGNNNCTG	1 cut(s)	127
PsuI	RGATCY	1 cut(s)	87
PsyI	GACNNNGTC	1 cut(s)	74
RsaI	GTAC	1 cut(s)	266
RsaNI	GTAC	1 cut(s)	265
SalI	GTCGAC	1 cut(s)	193
SatI	GCNGC	3 cut(s)	58, 122, 146
Sau3AI	GATC	3 cut(s)	87, 129, 378
Sau96I	GGNCC	2 cut(s)	115, 366
SchI	GAGTC	2 cut(s)	190, 200
ScrFI	CCNGG	4 cut(s)	28, 92, 113, 221
SetI	ASST	3 cut(s)	51, 150, 172
SfaNI	GCATC	1 cut(s)	319
SfcI	CTRYAG	2 cut(s)	66, 328
Sfr274I	CTCGAG	1 cut(s)	44
SinI	GGWCC	1 cut(s)	115
SlaI	CTCGAG	1 cut(s)	44
SmlI	CTYRAG	1 cut(s)	44
SmoI	CTYRAG	1 cut(s)	44
SspMI	CTAG	1 cut(s)	167
StyD4I	CCNGG	4 cut(s)	26, 90, 111, 219
TaaI	ACNGT	1 cut(s)	329
TaqI	TCGA	5 cut(s)	45, 51, 150, 194, 234
TatI	WGTACW	1 cut(s)	264
TscAI	CASTG	1 cut(s)	327
TseI	GCWGC	3 cut(s)	57, 121, 145
TspDTI	ATGAA	1 cut(s)	191
TspRI	CASTG	1 cut(s)	327
Tth111I	GACNNNGTC	1 cut(s)	74
VpaK11BI	GGWCC	1 cut(s)	115
XhoI	CTCGAG	1 cut(s)	44
XmiI	GTMKAC	1 cut(s)	194
XspI	CTAG	1 cut(s)	167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.