MD08G1095700.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001910
Physical Location & Seq
Forward (+)
8025933 .. 8028109
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1095700.v1.1.491

Sequence Viewer

Length: 483 bp
ATGTCTGGAGGAATCGCGCGCGGTCGTCTCGCCGAGGAGCGAAAAGCGTGGCGCAAGAACCACCCACATGGTTTTGTGGCGAAGCCGGAGACTGCTCCGGATGGCTCCGTTAATTTGATGGTGTGGCAGTGCATCATCCCCGGCAAACCATCTACTGATTGGGAAGGTGGCTACTTTCCACTTACACTTAACTTCACTGAGGATTACCCCAGCAAGCCCCCAAAATGCAAGTTCCCACAGGGCTTTTTCCACCCAAATGTGTATCCTTCTGGAACTGTTTGTCTTTCAATTCTCAATGAGGACAAAGGTTGGAGGCCGGCCATCACAGTGAAGCAAATCCTAGTAGGCATTCAGGATTTGCTAGGCCAGCCTAATCCTGCTGATCCGGCACAAACTGAAGGTTATCAACTCTTTATCCAGGAACCAGCGGAGTACAAGCGAAGAGTGCGACAGCAGGCAAAGCAATATCCACCTGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko04064 ko04120 ko05206 map03013 map04064 map04120 map05206
Pfam Domains
Protein Families
UQ_con

Protein Analysis

161

Amino Acids

17.99

Weight (kDa)

8.99

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 152 1.1e-45 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 17, 19, 21
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 2 cut(s) 21, 428
AclWI GGATC 1 cut(s) 377
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 434
AgsI TTSAA 1 cut(s) 288
AjnI CCWGG 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 32, 83
AlwI GGATC 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 3 cut(s) 314, 318, 364
AspLEI GCGC 3 cut(s) 19, 21, 54
AsuC2I CCSGG 1 cut(s) 141
BccI CCATC 4 cut(s) 95, 112, 157, 329
BciT130I CCWGG 1 cut(s) 419
BciVI GTATCC 1 cut(s) 273
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 32, 83
BfaI CTAG 2 cut(s) 341, 362
BfuI GTATCC 1 cut(s) 273
Bme1390I CCNGG 2 cut(s) 141, 419
BmiI GGNNCC 2 cut(s) 106, 423
BmrFI CCNGG 2 cut(s) 141, 419
BmsI GCATC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 27
BpuMI CCSGG 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 33, 139
BsaWI WCCGGW 1 cut(s) 97
Bse118I RCCGGY 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 33, 139
BseGI GGATG 2 cut(s) 106, 135
BseMII CTCAG 1 cut(s) 189
BsePI GCGCGC 1 cut(s) 17
BseRI GAGGAG 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 3 cut(s) 17, 19, 21
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 316, 320, 366
BsiEI CGRYCG 1 cut(s) 25
BsiSI CCGG 5 cut(s) 86, 98, 141, 317, 386
BsmAI GTCTC 2 cut(s) 32, 83
BsmBI CGTCTC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 3 cut(s) 316, 320, 366
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 2 cut(s) 21, 428
BspANI GGCC 3 cut(s) 316, 320, 366
BspCNI CTCAG 1 cut(s) 190
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 3 cut(s) 17, 19, 21
BspLI GGNNCC 2 cut(s) 106, 423
BspPI GGATC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 2 cut(s) 33, 139
BssHII GCGCGC 1 cut(s) 17
BssMI GATC 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 277, 328
Bst6I CTCTTC 1 cut(s) 436
BstC8I GCNNGC 5 cut(s) 19, 215, 318, 368, 456
BstDEI CTNAG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 106, 135
BstFNI CGCG 3 cut(s) 17, 19, 21
BstHHI GCGC 3 cut(s) 19, 21, 54
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 2 cut(s) 32, 83
BstMBI GATC 1 cut(s) 382
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 4 cut(s) 367, 386, 445, 460
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 2 cut(s) 139, 417
BstUI CGCG 3 cut(s) 17, 19, 21
BstXI CCANNNNNNTGG 1 cut(s) 68
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 3 cut(s) 316, 320, 366
BtsCI GGATG 2 cut(s) 106, 135
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 3 cut(s) 134, 195, 333
Cac8I GCNNGC 5 cut(s) 19, 215, 318, 368, 456
CfoI GCGC 3 cut(s) 19, 21, 54
Cfr10I RCCGGY 1 cut(s) 316
Csp6I GTAC 1 cut(s) 433
CviAII CATG 1 cut(s) 68
CviJI RGCY 9 cut(s) 85, 105, 171, 217, 243, 316, 320, 366, 370
CviKI_1 RGCY 9 cut(s) 85, 105, 171, 217, 243, 316, 320, 366, 370
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 198
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 417
EcoRII CCWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 1 cut(s) 71
FaiI YATR 3 cut(s) 69, 479, 481
FatI CATG 1 cut(s) 67
FokI GGATG 2 cut(s) 113, 122
FseI GGCCGGCC 1 cut(s) 320
FspBI CTAG 2 cut(s) 341, 362
GlaI GCGC 3 cut(s) 18, 20, 53
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 3 cut(s) 316, 320, 366
HapII CCGG 5 cut(s) 86, 98, 141, 317, 386
HhaI GCGC 3 cut(s) 19, 21, 54
Hin1II CATG 1 cut(s) 71
Hin6I GCGC 3 cut(s) 17, 19, 52
HinP1I GCGC 3 cut(s) 17, 19, 52
HinfI GANTC 1 cut(s) 12
HpaII CCGG 5 cut(s) 86, 98, 141, 317, 386
Hpy188III TCNNGA 4 cut(s) 6, 98, 270, 353
HpyAV CCTTC 3 cut(s) 158, 276, 392
HpyCH4III ACNGT 2 cut(s) 277, 328
HpyCH4V TGCA 2 cut(s) 132, 228
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 386, 445, 460
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 1 cut(s) 71
HspAI GCGC 3 cut(s) 17, 19, 52
Kpn2I TCCGGA 1 cut(s) 97
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 3 cut(s) 37, 100, 110
LweI GCATC 1 cut(s) 141
MaeI CTAG 2 cut(s) 341, 362
MalI GATC 1 cut(s) 384
MauBI CGCGCGCG 1 cut(s) 17
MboI GATC 1 cut(s) 382
MboII GAAGA 1 cut(s) 453
MluCI AATT 2 cut(s) 112, 288
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 4 cut(s) 28, 193, 292, 306
MroI TCCGGA 1 cut(s) 97
MroNI GCCGGC 1 cut(s) 316
MseI TTAA 2 cut(s) 111, 189
MslI CAYNNNNRTG 3 cut(s) 66, 255, 326
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 5 cut(s) 86, 98, 141, 317, 386
MspR9I CCNGG 2 cut(s) 141, 419
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 3 cut(s) 17, 19, 21
MwoI GCNNNNNNNGC 4 cut(s) 367, 386, 445, 460
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 1 cut(s) 141
NdeII GATC 1 cut(s) 382
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 1 cut(s) 71
NlaIV GGNNCC 2 cut(s) 106, 423
NmeAIII GCCGAG 1 cut(s) 58
PauI GCGCGC 1 cut(s) 17
PctI GAATGC 1 cut(s) 348
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 106, 423
PteI GCGCGC 1 cut(s) 17
RigI GGCCGGCC 1 cut(s) 320
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 3 cut(s) 66, 255, 326
SaqAI TTAA 2 cut(s) 111, 189
Sau3AI GATC 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 141, 419
SetI ASST 4 cut(s) 169, 310, 403, 475
SfaNI GCATC 1 cut(s) 141
SmiMI CAYNNNNRTG 3 cut(s) 66, 255, 326
Sse9I AATT 2 cut(s) 112, 288
SsiI CCGC 2 cut(s) 21, 428
SspMI CTAG 2 cut(s) 341, 362
StyD4I CCNGG 2 cut(s) 139, 417
TaaI ACNGT 2 cut(s) 277, 328
TasI AATT 2 cut(s) 112, 288
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 2 cut(s) 111, 189
Tru9I TTAA 2 cut(s) 111, 189
TscAI CASTG 3 cut(s) 134, 202, 333
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 3 cut(s) 134, 202, 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 156
XspI CTAG 2 cut(s) 341, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.