Belongs to the ubiquitin-conjugating enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 3 cut(s) | 210, 219, 224 |
| AcsI | RAATTY | 3 cut(s) | 77, 88, 183 |
| AcuI | CTGAAG | 1 cut(s) | 25 |
| AfaI | GTAC | 1 cut(s) | 20 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 145 |
| AleI | CACNNNNGTG | 1 cut(s) | 42 |
| Alw26I | GTCTC | 1 cut(s) | 154 |
| AlwI | GGATC | 3 cut(s) | 210, 219, 224 |
| ApoI | RAATTY | 3 cut(s) | 77, 88, 183 |
| BbsI | GAAGAC | 1 cut(s) | 54 |
| BccI | CCATC | 1 cut(s) | 121 |
| BcoDI | GTCTC | 1 cut(s) | 154 |
| BfaI | CTAG | 1 cut(s) | 57 |
| BmrI | ACTGGG | 1 cut(s) | 16 |
| BmuI | ACTGGG | 1 cut(s) | 16 |
| BpiI | GAAGAC | 1 cut(s) | 54 |
| BsaI | GGTCTC | 1 cut(s) | 154 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 32 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 145 |
| Bse1I | ACTGG | 2 cut(s) | 11, 213 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 145 |
| BseNI | ACTGG | 2 cut(s) | 11, 213 |
| BseYI | CCCAGC | 1 cut(s) | 82 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 145 |
| BsmAI | GTCTC | 1 cut(s) | 154 |
| BsmI | GAATGC | 1 cut(s) | 195 |
| Bso31I | GGTCTC | 1 cut(s) | 154 |
| Bsp143I | GATC | 3 cut(s) | 202, 211, 229 |
| BspPI | GGATC | 3 cut(s) | 210, 219, 224 |
| BspTNI | GGTCTC | 1 cut(s) | 154 |
| BsrI | ACTGG | 2 cut(s) | 11, 213 |
| BssMI | GATC | 3 cut(s) | 202, 211, 229 |
| Bst4CI | ACNGT | 3 cut(s) | 124, 152, 175 |
| BstC8I | GCNNGC | 1 cut(s) | 62 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 143 |
| BstKTI | GATC | 3 cut(s) | 205, 214, 232 |
| BstMAI | GTCTC | 1 cut(s) | 154 |
| BstMBI | GATC | 3 cut(s) | 202, 211, 229 |
| BstV2I | GAAGAC | 1 cut(s) | 54 |
| BstX2I | RGATCY | 1 cut(s) | 202 |
| BstYI | RGATCY | 1 cut(s) | 202 |
| BtsIMutI | CAGTG | 2 cut(s) | 49, 157 |
| Cac8I | GCNNGC | 1 cut(s) | 62 |
| Csp6I | GTAC | 1 cut(s) | 19 |
| CviJI | RGCY | 2 cut(s) | 64, 217 |
| CviKI_1 | RGCY | 2 cut(s) | 64, 217 |
| CviQI | GTAC | 1 cut(s) | 19 |
| DpnI | GATC | 3 cut(s) | 204, 213, 231 |
| DpnII | GATC | 3 cut(s) | 202, 211, 229 |
| Eco31I | GGTCTC | 1 cut(s) | 154 |
| Eco57I | CTGAAG | 1 cut(s) | 25 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 143 |
| FaiI | YATR | 1 cut(s) | 109 |
| FspBI | CTAG | 1 cut(s) | 57 |
| GsaI | CCCAGC | 1 cut(s) | 86 |
| Hpy188I | TCNGA | 1 cut(s) | 229 |
| Hpy188III | TCNNGA | 2 cut(s) | 117, 200 |
| HpyCH4III | ACNGT | 3 cut(s) | 124, 152, 175 |
| HpyCH4V | TGCA | 1 cut(s) | 75 |
| Kzo9I | GATC | 3 cut(s) | 202, 211, 229 |
| LpnPI | CCDG | 5 cut(s) | 96, 102, 177, 185, 194 |
| MaeI | CTAG | 1 cut(s) | 57 |
| MalI | GATC | 3 cut(s) | 204, 213, 231 |
| MboI | GATC | 3 cut(s) | 202, 211, 229 |
| MboII | GAAGA | 2 cut(s) | 59, 85 |
| MflI | RGATCY | 1 cut(s) | 202 |
| MluCI | AATT | 4 cut(s) | 77, 88, 168, 183 |
| MmeI | TCCRAC | 1 cut(s) | 137 |
| MnlI | CCTC | 4 cut(s) | 4, 75, 139, 235 |
| MseI | TTAA | 1 cut(s) | 141 |
| MslI | CAYNNNNRTG | 2 cut(s) | 42, 102 |
| Mva1269I | GAATGC | 1 cut(s) | 195 |
| NdeII | GATC | 3 cut(s) | 202, 211, 229 |
| OliI | CACNNNNGTG | 1 cut(s) | 42 |
| PctI | GAATGC | 1 cut(s) | 195 |
| PspFI | CCCAGC | 1 cut(s) | 82 |
| PsuI | RGATCY | 1 cut(s) | 202 |
| RsaI | GTAC | 1 cut(s) | 20 |
| RsaNI | GTAC | 1 cut(s) | 19 |
| RseI | CAYNNNNRTG | 2 cut(s) | 42, 102 |
| SaqAI | TTAA | 1 cut(s) | 141 |
| Sau3AI | GATC | 3 cut(s) | 202, 211, 229 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 42, 102 |
| Sse9I | AATT | 4 cut(s) | 77, 88, 168, 183 |
| SspMI | CTAG | 1 cut(s) | 57 |
| TaaI | ACNGT | 3 cut(s) | 124, 152, 175 |
| TasI | AATT | 4 cut(s) | 77, 88, 168, 183 |
| Tru1I | TTAA | 1 cut(s) | 141 |
| Tru9I | TTAA | 1 cut(s) | 141 |
| TscAI | CASTG | 2 cut(s) | 49, 157 |
| TspRI | CASTG | 2 cut(s) | 49, 157 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 143 |
| XapI | RAATTY | 3 cut(s) | 77, 88, 183 |
| XspI | CTAG | 1 cut(s) | 57 |