Belongs to the ubiquitin-conjugating enzyme family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 290 |
| AclWI | GGATC | 1 cut(s) | 165 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 99 |
| AjnI | CCWGG | 1 cut(s) | 152 |
| AluBI | AGCT | 2 cut(s) | 64, 244 |
| AluI | AGCT | 2 cut(s) | 64, 244 |
| Alw21I | GWGCWC | 2 cut(s) | 66, 129 |
| AlwI | GGATC | 1 cut(s) | 165 |
| AoxI | GGCC | 1 cut(s) | 269 |
| ApeKI | GCWGC | 2 cut(s) | 315, 318 |
| AspS9I | GGNCC | 3 cut(s) | 102, 131, 269 |
| AsuHPI | GGTGA | 1 cut(s) | 130 |
| AvaII | GGWCC | 2 cut(s) | 102, 131 |
| BanII | GRGCYC | 1 cut(s) | 66 |
| Bbv12I | GWGCWC | 2 cut(s) | 66, 129 |
| BbvI | GCAGC | 1 cut(s) | 327 |
| BccI | CCATC | 2 cut(s) | 101, 113 |
| BciT130I | CCWGG | 1 cut(s) | 154 |
| BfaI | CTAG | 1 cut(s) | 99 |
| BisI | GCNGC | 2 cut(s) | 316, 319 |
| BlsI | GCNGC | 2 cut(s) | 317, 320 |
| Bme1390I | CCNGG | 1 cut(s) | 154 |
| Bme18I | GGWCC | 2 cut(s) | 102, 131 |
| BmgT120I | GGNCC | 3 cut(s) | 102, 131, 269 |
| BmrFI | CCNGG | 1 cut(s) | 154 |
| BsaJI | CCNNGG | 1 cut(s) | 153 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 99 |
| BseBI | CCWGG | 1 cut(s) | 154 |
| BseDI | CCNNGG | 1 cut(s) | 153 |
| BseGI | GGATG | 1 cut(s) | 231 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 99 |
| BseXI | GCAGC | 1 cut(s) | 327 |
| BseYI | CCCAGC | 1 cut(s) | 311 |
| BshFI | GGCC | 1 cut(s) | 271 |
| BsiHKAI | GWGCWC | 2 cut(s) | 66, 129 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 99 |
| BsnI | GGCC | 1 cut(s) | 271 |
| Bsp1286I | GDGCHC | 2 cut(s) | 66, 129 |
| Bsp143I | GATC | 1 cut(s) | 157 |
| BspANI | GGCC | 1 cut(s) | 271 |
| BspPI | GGATC | 1 cut(s) | 165 |
| BssECI | CCNNGG | 1 cut(s) | 153 |
| BssMI | GATC | 1 cut(s) | 157 |
| BssNAI | GTATAC | 1 cut(s) | 291 |
| Bst1107I | GTATAC | 1 cut(s) | 291 |
| Bst2UI | CCWGG | 1 cut(s) | 154 |
| Bst4CI | ACNGT | 1 cut(s) | 131 |
| BstDEI | CTNAG | 1 cut(s) | 245 |
| BstF5I | GGATG | 1 cut(s) | 231 |
| BstKTI | GATC | 1 cut(s) | 160 |
| BstMBI | GATC | 1 cut(s) | 157 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 277 |
| BstNI | CCWGG | 1 cut(s) | 154 |
| BstSCI | CCNGG | 1 cut(s) | 152 |
| BstV1I | GCAGC | 1 cut(s) | 327 |
| BstZ17I | GTATAC | 1 cut(s) | 291 |
| BsuRI | GGCC | 1 cut(s) | 271 |
| BtsCI | GGATG | 1 cut(s) | 231 |
| Cfr13I | GGNCC | 3 cut(s) | 102, 131, 269 |
| CviAII | CATG | 2 cut(s) | 205, 263 |
| CviJI | RGCY | 4 cut(s) | 64, 244, 271, 321 |
| CviKI_1 | RGCY | 4 cut(s) | 64, 244, 271, 321 |
| DdeI | CTNAG | 1 cut(s) | 245 |
| DpnI | GATC | 1 cut(s) | 159 |
| DpnII | GATC | 1 cut(s) | 157 |
| Ecl136II | GAGCTC | 1 cut(s) | 64 |
| Eco24I | GRGCYC | 1 cut(s) | 66 |
| Eco47I | GGWCC | 2 cut(s) | 102, 131 |
| Eco53kI | GAGCTC | 1 cut(s) | 64 |
| EcoICRI | GAGCTC | 1 cut(s) | 64 |
| EcoO109I | RGGNCCY | 1 cut(s) | 269 |
| EcoRII | CCWGG | 1 cut(s) | 152 |
| EcoT38I | GRGCYC | 1 cut(s) | 66 |
| FaeI | CATG | 2 cut(s) | 208, 266 |
| FaiI | YATR | 5 cut(s) | 206, 256, 264, 287, 291 |
| FatI | CATG | 2 cut(s) | 204, 262 |
| FblI | GTMKAC | 1 cut(s) | 290 |
| Fnu4HI | GCNGC | 2 cut(s) | 316, 319 |
| FokI | GGATG | 1 cut(s) | 238 |
| FriOI | GRGCYC | 1 cut(s) | 66 |
| Fsp4HI | GCNGC | 2 cut(s) | 316, 319 |
| FspBI | CTAG | 1 cut(s) | 99 |
| GluI | GCNGC | 2 cut(s) | 316, 319 |
| GsaI | CCCAGC | 1 cut(s) | 315 |
| HaeIII | GGCC | 1 cut(s) | 271 |
| Hin1II | CATG | 2 cut(s) | 208, 266 |
| HindIII | AAGCTT | 1 cut(s) | 242 |
| HphI | GGTGA | 1 cut(s) | 130 |
| Hpy166II | GTNNAC | 2 cut(s) | 291, 303 |
| Hpy188I | TCNGA | 1 cut(s) | 148 |
| Hpy188III | TCNNGA | 1 cut(s) | 118 |
| Hpy8I | GTNNAC | 2 cut(s) | 291, 303 |
| HpyAV | CCTTC | 2 cut(s) | 115, 223 |
| HpyCH4III | ACNGT | 1 cut(s) | 131 |
| HpyCH4IV | ACGT | 1 cut(s) | 162 |
| HpyCH4V | TGCA | 1 cut(s) | 258 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 277 |
| HpyF3I | CTNAG | 1 cut(s) | 245 |
| HpySE526I | ACGT | 1 cut(s) | 162 |
| Hsp92II | CATG | 2 cut(s) | 208, 266 |
| Kzo9I | GATC | 1 cut(s) | 157 |
| LmnI | GCTCC | 1 cut(s) | 124 |
| LpnPI | CCDG | 4 cut(s) | 90, 139, 166, 325 |
| Lsp1109I | GCAGC | 1 cut(s) | 327 |
| MaeI | CTAG | 1 cut(s) | 99 |
| MaeII | ACGT | 1 cut(s) | 162 |
| MalI | GATC | 1 cut(s) | 159 |
| MboI | GATC | 1 cut(s) | 157 |
| MboII | GAAGA | 1 cut(s) | 220 |
| MhlI | GDGCHC | 2 cut(s) | 66, 129 |
| MnlI | CCTC | 5 cut(s) | 144, 176, 192, 213, 228 |
| MseI | TTAA | 2 cut(s) | 165, 179 |
| MspR9I | CCNGG | 1 cut(s) | 154 |
| MvaI | CCWGG | 1 cut(s) | 154 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 277 |
| NdeII | GATC | 1 cut(s) | 157 |
| NlaIII | CATG | 2 cut(s) | 208, 266 |
| PkrI | GCNGC | 2 cut(s) | 317, 320 |
| Psp124BI | GAGCTC | 1 cut(s) | 66 |
| Psp6I | CCWGG | 1 cut(s) | 152 |
| PspFI | CCCAGC | 1 cut(s) | 311 |
| PspGI | CCWGG | 1 cut(s) | 152 |
| PspPI | GGNCC | 3 cut(s) | 102, 131, 269 |
| SacI | GAGCTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 2 cut(s) | 165, 179 |
| SatI | GCNGC | 2 cut(s) | 316, 319 |
| Sau3AI | GATC | 1 cut(s) | 157 |
| Sau96I | GGNCC | 3 cut(s) | 102, 131, 269 |
| ScrFI | CCNGG | 1 cut(s) | 154 |
| SduI | GDGCHC | 2 cut(s) | 66, 129 |
| SetI | ASST | 7 cut(s) | 23, 66, 155, 165, 224, 246, 330 |
| SinI | GGWCC | 2 cut(s) | 102, 131 |
| SspMI | CTAG | 1 cut(s) | 99 |
| SstI | GAGCTC | 1 cut(s) | 66 |
| StyD4I | CCNGG | 1 cut(s) | 152 |
| TaaI | ACNGT | 1 cut(s) | 131 |
| TaiI | ACGT | 1 cut(s) | 165 |
| Tru1I | TTAA | 2 cut(s) | 165, 179 |
| Tru9I | TTAA | 2 cut(s) | 165, 179 |
| TseI | GCWGC | 2 cut(s) | 315, 318 |
| TspDTI | ATGAA | 3 cut(s) | 65, 221, 242 |
| VpaK11BI | GGWCC | 2 cut(s) | 102, 131 |
| XmiI | GTMKAC | 1 cut(s) | 290 |
| XspI | CTAG | 1 cut(s) | 99 |