Rh6AG476300

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001910
Physical Location & Seq
Reverse (-)
65801758 .. 65805137
3380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG476300.1

Sequence Viewer

Length: 438 bp
ATGTCTGGAGGAATCGCGCGTGGTCGCCTCGCGGAGGAACGAAAATCCTGGCGTAAGAATCACCCCCATGGTTTTGTGGCGAAGCCAGAGACAGGTGCAGATGGCTCCGTCAATTTGATGGTTTGGCAGTGCTTAATCCCTGGAAAACCCGCTACTGATTGGGAGGGTGGCAACTATCCACTTACACTCCAGTTCAGTGAGGATTACCCCAGCAAACCCCCAAAGTGCAAGTTCCCTCAGGGTTTTTTCCACCCTAATGTTTATCCTTCTGGAACCGTTTGCCTTTCAATTCTCAATGAGGATAAGGGGTGGAGGCCTGCCATTACTGTGAAGCAAATCCTAGTAGGCATTCAGGACTTGCTAGACCAGCCTAACCCTGCTGATCCTGCACAAACTGAGGGTTATCAGCTCTTCATCCAGGTTTGTTTTGAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko04064 ko04120 ko05206 map03013 map04064 map04120 map05206
Pfam Domains
Protein Families
UQ_con

Protein Analysis

145

Amino Acids

16.16

Weight (kDa)

6.28

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 139 2.5e-42 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 17, 19, 32
AciI CCGC 2 cut(s) 32, 150
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 34, 92
AgsI TTSAA 2 cut(s) 288, 431
AjnI CCWGG 3 cut(s) 47, 139, 417
AluBI AGCT 1 cut(s) 409
AluI AGCT 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 377
AoxI GGCC 1 cut(s) 314
ApoI RAATTY 1 cut(s) 431
AspLEI GCGC 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 53
AxyI CCTNAGG 1 cut(s) 237
BccI CCATC 2 cut(s) 95, 112
BciT130I CCWGG 3 cut(s) 49, 141, 419
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 3 cut(s) 341, 362, 436
Bme1390I CCNGG 3 cut(s) 49, 141, 419
BmiI GGNNCC 2 cut(s) 106, 274
BmrFI CCNGG 3 cut(s) 49, 141, 419
BpmI CTGGAG 2 cut(s) 27, 173
BsaJI CCNNGG 2 cut(s) 67, 139
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 92
Bse1I ACTGG 1 cut(s) 190
Bse21I CCTNAGG 1 cut(s) 237
BseBI CCWGG 3 cut(s) 49, 141, 419
BseDI CCNNGG 2 cut(s) 67, 139
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 34, 92
BseMII CTCAG 2 cut(s) 251, 387
BseNI ACTGG 1 cut(s) 190
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 2 cut(s) 117, 372
Bsh1236I CGCG 3 cut(s) 17, 19, 32
BshFI GGCC 1 cut(s) 316
BslI CCNNNNNNNGG 2 cut(s) 34, 92
BsmAI GTCTC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 316
Bsp143I GATC 1 cut(s) 382
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 2 cut(s) 32, 150
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 2 cut(s) 250, 388
BspFNI CGCG 3 cut(s) 17, 19, 32
BspLI GGNNCC 2 cut(s) 106, 274
BspPI GGATC 1 cut(s) 377
BspQI GCTCTTC 1 cut(s) 416
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 67, 139
BssMI GATC 1 cut(s) 382
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 3 cut(s) 49, 141, 419
Bst4CI ACNGT 2 cut(s) 277, 328
Bst6I CTCTTC 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 2 cut(s) 237, 396
BstDSI CCRYGG 1 cut(s) 67
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 3 cut(s) 17, 19, 32
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 2 cut(s) 367, 386
BstNI CCWGG 3 cut(s) 49, 141, 419
BstSCI CCNGG 3 cut(s) 47, 139, 417
BstUI CGCG 3 cut(s) 17, 19, 32
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 1 cut(s) 316
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 134, 202
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 1 cut(s) 19
CviAII CATG 1 cut(s) 68
CviJI RGCY 5 cut(s) 85, 105, 316, 370, 409
CviKI_1 RGCY 5 cut(s) 85, 105, 316, 370, 409
DdeI CTNAG 2 cut(s) 237, 396
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 67
Eco147I AGGCCT 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 237
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 3 cut(s) 47, 139, 417
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 1 cut(s) 71
FaiI YATR 1 cut(s) 69
FatI CATG 1 cut(s) 67
FauI CCCGC 1 cut(s) 157
FokI GGATG 1 cut(s) 401
FspBI CTAG 3 cut(s) 341, 362, 436
GlaI GCGC 1 cut(s) 18
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 2 cut(s) 27, 173
HaeIII GGCC 1 cut(s) 316
HhaI GCGC 1 cut(s) 19
Hin1II CATG 1 cut(s) 71
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 2 cut(s) 12, 58
HphI GGTGA 1 cut(s) 53
Hpy188III TCNNGA 3 cut(s) 6, 270, 353
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 2 cut(s) 277, 328
HpyCH4V TGCA 3 cut(s) 98, 228, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 386
HpyF3I CTNAG 2 cut(s) 237, 396
Hsp92II CATG 1 cut(s) 71
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 382
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 1 cut(s) 110
MaeI CTAG 3 cut(s) 341, 362, 436
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 1 cut(s) 403
MluCI AATT 3 cut(s) 112, 288, 431
MnlI CCTC 8 cut(s) 28, 38, 157, 193, 246, 292, 306, 391
MseI TTAA 1 cut(s) 134
MslI CAYNNNNRTG 3 cut(s) 66, 255, 326
MspR9I CCNGG 3 cut(s) 49, 141, 419
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 3 cut(s) 49, 141, 419
MvnI CGCG 3 cut(s) 17, 19, 32
MwoI GCNNNNNNNGC 2 cut(s) 367, 386
NcoI CCATGG 1 cut(s) 67
NdeII GATC 1 cut(s) 382
NlaIII CATG 1 cut(s) 71
NlaIV GGNNCC 2 cut(s) 106, 274
PceI AGGCCT 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 416
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 12, 58
Psp6I CCWGG 3 cut(s) 47, 139, 417
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 3 cut(s) 47, 139, 417
PspN4I GGNNCC 2 cut(s) 106, 274
RseI CAYNNNNRTG 3 cut(s) 66, 255, 326
SapI GCTCTTC 1 cut(s) 416
SaqAI TTAA 1 cut(s) 134
Sau3AI GATC 1 cut(s) 382
ScrFI CCNGG 3 cut(s) 49, 141, 419
SetI ASST 3 cut(s) 97, 411, 423
SmiMI CAYNNNNRTG 3 cut(s) 66, 255, 326
Sse9I AATT 3 cut(s) 112, 288, 431
SseBI AGGCCT 1 cut(s) 316
SsiI CCGC 2 cut(s) 32, 150
SspMI CTAG 3 cut(s) 341, 362, 436
StuI AGGCCT 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 47, 139, 417
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 2 cut(s) 277, 328
TasI AATT 3 cut(s) 112, 288, 431
TfiI GAWTC 2 cut(s) 12, 58
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TscAI CASTG 2 cut(s) 134, 202
TspDTI ATGAA 1 cut(s) 403
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 134, 202
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 1 cut(s) 431
XspI CTAG 3 cut(s) 341, 362, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.