MD08G1189800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005641
Physical Location & Seq
Reverse (-)
23942701 .. 23944625
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1189800.v1.1.491

Sequence Viewer

Length: 720 bp
ATGTCGATCAGAAGCTCAATAAGTTCTCCTTGCAGTTCTAGACATTTGAAGGCTTCAGTTTTAAGACCTAGTTGTCTGAGTACTAATCATCGGGACGGATGTCGTTTTCTCGGAAATCGGAGCTTCTTGCAGCCCCTTATCCTGAAGGTCGAAAGTGGTAGCCAGAAAAAGATGGCCGTGGTTGTCAATAGTGTTGAACCAGGAACTCCTCTTCCTTCTGATCCTTCCGGTACTAATTGGAAACTATGGCTTGTGGGGATGCTTTTCTCAATAGCTATACCCTTTTGGAAGAACAAATGGTGGCCATTGCAAAATATCAAAGAGAGATTTTCGACGGCGCTGGACAAAGTGGAAGATGTGGCAGAAATGGTAGAGAAGGTTGCTGGGGAAGTGGAGAAGGTGGCTGACGACATTGCCGATCATCTTCCGGAAGGGAAACTTCAAAGGGCAGCAAGGATTGTTGAAAGCGTGGCAAGAGAAGCAGGCAAGGATGCTGCGCTAGCAGATGATCTCATTGACAAGGTTGACGAAGTCGAAAAGGAGGTGGATTCTATCATTCAGCAAGCCAATAAGAAAATCGAAGAAGAAAATGATACCGAAGATGAGACGGCAAAAATTAAAGATGCGACTGATATTAAAACAAAGATAGCGGAGATCAAAGCAACGAATGTTGTTAAAAAAGAGACAATAAAAACTGATGCAGTAGATGACTCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.48

Weight (kDa)

5.45

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 72
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 1 cut(s) 650
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 2 cut(s) 174, 302
AcuI CTGAAG 2 cut(s) 39, 164
AfaI GTAC 2 cut(s) 82, 232
AgsI TTSAA 4 cut(s) 49, 197, 443, 464
AjnI CCWGG 1 cut(s) 199
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 3 cut(s) 15, 123, 275
AluI AGCT 3 cut(s) 15, 123, 275
Alw26I GTCTC 2 cut(s) 599, 677
AlwI GGATC 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 427
AoxI GGCC 2 cut(s) 174, 302
ApeKI GCWGC 3 cut(s) 130, 449, 494
AspLEI GCGC 2 cut(s) 340, 499
AsuNHI GCTAGC 1 cut(s) 499
BalI TGGCCA 1 cut(s) 304
BbvI GCAGC 3 cut(s) 142, 461, 481
BccI CCATC 1 cut(s) 166
BceAI ACGGC 3 cut(s) 161, 351, 624
BciT130I CCWGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 599, 677
BfaI CTAG 3 cut(s) 39, 69, 500
BfoI RGCGCY 1 cut(s) 341
BisI GCNGC 3 cut(s) 131, 450, 495
BlsI GCNGC 3 cut(s) 132, 451, 496
BmcAI AGTACT 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 4 cut(s) 249, 481, 613, 688
BmtI GCTAGC 1 cut(s) 503
BoxI GACNNNNGTC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 2 cut(s) 227, 427
Bse3DI GCAATG 2 cut(s) 305, 411
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 3 cut(s) 104, 264, 496
BseMI GCAATG 2 cut(s) 305, 411
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 3 cut(s) 142, 461, 481
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 2 cut(s) 176, 304
BsiSI CCGG 2 cut(s) 228, 428
BslFI GGGAC 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 599, 677
BsmBI CGTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 2 cut(s) 176, 304
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 5 cut(s) 6, 220, 418, 508, 654
BspACI CCGC 1 cut(s) 650
BspANI GGCC 2 cut(s) 176, 304
BspCNI CTCAG 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 503
BspPI GGATC 1 cut(s) 215
BsrDI GCAATG 2 cut(s) 305, 411
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 5 cut(s) 6, 220, 418, 508, 654
Bst2UI CCWGG 1 cut(s) 201
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 484, 501, 564
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 104, 264, 496
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 2 cut(s) 340, 499
BstKTI GATC 5 cut(s) 9, 223, 421, 511, 657
BstMAI GTCTC 2 cut(s) 599, 677
BstMBI GATC 5 cut(s) 6, 220, 418, 508, 654
BstMWI GCNNNNNNNGC 2 cut(s) 479, 500
BstNI CCWGG 1 cut(s) 201
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 199
BstV1I GCAGC 3 cut(s) 142, 461, 481
BsuRI GGCC 2 cut(s) 176, 304
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 104, 264, 496
Cac8I GCNNGC 3 cut(s) 484, 501, 564
CfoI GCGC 2 cut(s) 340, 499
Csp6I GTAC 2 cut(s) 81, 231
CviQI GTAC 2 cut(s) 81, 231
DdeI CTNAG 1 cut(s) 77
DpnI GATC 5 cut(s) 8, 222, 420, 510, 656
DpnII GATC 5 cut(s) 6, 220, 418, 508, 654
DrdI GACNNNNNNGTC 1 cut(s) 72
DseDI GACNNNNNNGTC 1 cut(s) 72
EaeI YGGCCR 2 cut(s) 174, 302
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco57I CTGAAG 2 cut(s) 39, 164
EcoRII CCWGG 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 599
FaiI YATR 2 cut(s) 247, 278
FalI AAGNNNNNCTT 4 cut(s) 13, 45, 423, 455
FaqI GGGAC 1 cut(s) 107
Fnu4HI GCNGC 3 cut(s) 131, 450, 495
FokI GGATG 3 cut(s) 111, 271, 503
Fsp4HI GCNGC 3 cut(s) 131, 450, 495
FspBI CTAG 3 cut(s) 39, 69, 500
GlaI GCGC 2 cut(s) 339, 498
GluI GCNGC 3 cut(s) 131, 450, 495
GsaI CCCAGC 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 341
HaeIII GGCC 2 cut(s) 176, 304
HapII CCGG 2 cut(s) 228, 428
HhaI GCGC 2 cut(s) 340, 499
Hin6I GCGC 2 cut(s) 338, 497
HinP1I GCGC 2 cut(s) 338, 497
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 2 cut(s) 548, 710
HpaII CCGG 2 cut(s) 228, 428
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 5 cut(s) 11, 78, 113, 120, 220
Hpy188III TCNNGA 4 cut(s) 39, 92, 142, 428
Hpy8I GTNNAC 1 cut(s) 526
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 7 cut(s) 43, 139, 225, 234, 370, 391, 425
HpyCH4V TGCA 4 cut(s) 33, 130, 310, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 500
HpyF3I CTNAG 1 cut(s) 77
HspAI GCGC 2 cut(s) 338, 497
Kpn2I TCCGGA 1 cut(s) 427
Kzo9I GATC 5 cut(s) 6, 220, 418, 508, 654
LmnI GCTCC 1 cut(s) 120
LpnPI CCDG 9 cut(s) 155, 176, 186, 213, 241, 326, 369, 441, 468
Lsp1109I GCAGC 3 cut(s) 142, 461, 481
LweI GCATC 4 cut(s) 249, 481, 613, 688
MaeI CTAG 3 cut(s) 39, 69, 500
MalI GATC 5 cut(s) 8, 222, 420, 510, 656
MboI GATC 5 cut(s) 6, 220, 418, 508, 654
MboII GAAGA 7 cut(s) 203, 301, 365, 416, 593, 596, 611
MlsI TGGCCA 1 cut(s) 304
MluCI AATT 2 cut(s) 235, 615
MluNI TGGCCA 1 cut(s) 304
MlyI GAGTC 1 cut(s) 704
MnlI CCTC 2 cut(s) 219, 535
Mox20I TGGCCA 1 cut(s) 304
MroI TCCGGA 1 cut(s) 427
MscI TGGCCA 1 cut(s) 304
MseI TTAA 4 cut(s) 62, 618, 636, 675
Msp20I TGGCCA 1 cut(s) 304
MspI CCGG 2 cut(s) 228, 428
MspR9I CCNGG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 479, 500
NdeII GATC 5 cut(s) 6, 220, 418, 508, 654
NheI GCTAGC 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 530
PkrI GCNGC 3 cut(s) 132, 451, 496
PleI GAGTC 1 cut(s) 704
PpsI GAGTC 1 cut(s) 704
PshAI GACNNNNGTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 199
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 2 cut(s) 82, 232
RsaNI GTAC 2 cut(s) 81, 231
SaqAI TTAA 4 cut(s) 62, 618, 636, 675
SatI GCNGC 3 cut(s) 131, 450, 495
Sau3AI GATC 5 cut(s) 6, 220, 418, 508, 654
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 1 cut(s) 704
ScrFI CCNGG 1 cut(s) 201
SetI ASST 9 cut(s) 17, 70, 125, 150, 277, 381, 402, 525, 546
SfaNI GCATC 4 cut(s) 249, 481, 613, 688
Sse9I AATT 2 cut(s) 235, 615
SsiI CCGC 1 cut(s) 650
SspMI CTAG 3 cut(s) 39, 69, 500
StyD4I CCNGG 1 cut(s) 199
TaqI TCGA 5 cut(s) 5, 150, 332, 534, 579
TasI AATT 2 cut(s) 235, 615
TatI WGTACW 1 cut(s) 80
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 4 cut(s) 62, 618, 636, 675
Tru9I TTAA 4 cut(s) 62, 618, 636, 675
TseI GCWGC 3 cut(s) 130, 449, 494
TspGWI ACGGA 1 cut(s) 111
Tth111I GACNNNGTC 1 cut(s) 530
XbaI TCTAGA 1 cut(s) 38
XspI CTAG 3 cut(s) 39, 69, 500
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.