Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003547

Physical Location & Seq

Genomic Coordinates Forward (+)

24960189 .. 24966406

Gene Structure 6218 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1194100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGCTTCAATCTCCGGCATTTACTCTGCTTCTCCGACGATCAAGCGCCAGCTGAAACCGGCGAGTTTGCTGCGGAAGGACTTCGTATCGGTTTCGGTTGCGTTTCCGGCCCGGGTCAAATCGGCGGATCGGGGCTTTTCTCTATCCGTGGCTAGGGAGGTCTCGGCGGAGTTAGCGAAGACCGATGGGGCTCCGAGTAAGATGAAGGAGCTGGTGAAGGACCCGCATGCGCTGTGGCGGAGGTACGTTGACTGGCTGTACCAGCACAAGGAGCTCGGGTTGTTTCTGGATGTGAGTCGGGTCGGGTTCACCGACGAGTTCGTTTCTGAAATGGAGCCCCGGTTCCAGGCGGCGTTCAAGGCTATGGAGGAGCTGGAGAAAGGCGCGATTGCTAATCCCGATGAGGGCCGCATGGTCGGCCATTACTGGCTCCGTAATCCTAAGCTTGCCCCCAACTCGTTCCTCAGGTTGCAGATTGAGAACACACTCGAGGCGCTGCTTAAGTTCTCAAACGACGTCGTCAGCGGTAAGATTAAGCCGCCATCTTCGCCGGAAGGGCGGTTTACTCAGGTGCTTTCAGTTGGAATTGGAGGTTCAGCACTTGGACCACAGTTCGTTGCTGAGGCATTAGCTCCTGATAACCCTCCTCTTAAGATAAGATTTATTGACAATACCGATCCAGCGGGAATTGATCATCAGATTGCACAGCTTGGTCCTGAGTTGGCTTCTACACTTGTCATTGTGATTTCAAAGAGTGGAGGTACTCCTGAAACGAGAAATGGGTTACTGGAAGTACAGAAGGCCTTCCGTGAGGCTGGCCTGAATTTTGCAAAACAGGGTGTTGCTATAACTCAAGAAAAATCATTATTGGACAACACCGCCAGAATTGAAGGATGGTTAGCTAGATTCCCCATGTTTGACTGGGTTGGTGGCAGAACATCTGAAATGTCTGCAGTTGGTCTTCTTCCGGCAGCACTGCAGGGAATTGATATTAAAGAAATGCTTGCTGGCGGACACTTGATGGATGAGGCAAACAGAACCACTGTTCTTAGGAATAACCCGGCTGCGTTGCTTGCTTTATGCTGGTATTGGGCTTCTGACGGTGTGGGATCAAAGGATATGGTTGTTCTTCCTTACAAGGACAGCCTATTATTATTTAGTCGGTATTTGCAACAGCTGGTCATGGAATCTATTGGGAAGGAATTTGACCTTAATGGTAATCGGGTGAATCAAGGACTTACTGTCTATGGAAATAAAGGGAGCACGGATCAGCATGCCTACATTCAACAACTGAGAGATGGTGTGCACAATTTCTTTGTGACATTCATCGAAGTACTACGTGATAGGCCCCCAGGTCATGATTGGGAGCTTGAACCTGGCGTCACATGTGGTGACTACCTGTTTGGAATGCTGCAGGGAACAAGGTCGGCTCTTTATTCAAACGAGCGGGAGTCAATTACAGTTACAGTGGAAGAAGTGACACCAAGATCTGTGGGTGCTCTCATTGCACTTTATGAGAGAGCAGTCGGGATATATGCTTCACTTGTAAACATTAACGCCTACCATCAACCTGGTGTTGAAGCTGGGAAAAAAGCAGCTGGAGAAGTATTAGCCCTTCAGAAGCGGGTTTTAGCAGTACTCAATGAGGCCAGCTGCAAGGATCCTGTTGAACCGTTGACGCTTGAAGAAGTAGCTGACCGGTGTCATGCTACTGAAGATATTGAAATGATCTACAAGATCATTGCTCACATGGCTGCCAACGACAGAGCAATCATCGCTGAAGGCAACTGCGGTTCACCACGAAGCATCAAAGTTTTCCTCGGAGAGTGCAACGTGGATGCCTTGTACGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009909 GO:0009911 GO:0009941 GO:0009987 GO:0016853 GO:0016860 GO:0016861 GO:0031967 GO:0031975 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PGI

Protein Analysis

618

Amino Acids

67.62

Weight (kDa)

5.72

Isoelectric Point (pI)

35.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGI	PF00342	121 - 447	1.8e-47	Phosphoglucose isomerase
PGI	PF00342	478 - 538	1.8e-09	Phosphoglucose isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24620 AT4G24620
fragaria_vesca	FvH4_5g35680 FvH4_5g35680
malus_domestica	MD08G1194100.v1.1 MD15G1382200.v1.1
prunus_persica	Prupe.1G528400_v2.0.a1 Prupe.1G528400_v2.0.a1
pyrus_communis	pycom15g34220
rosa_chinensis	RchiOBHm_Chr7g0237251 RchiOBHm_Chr7g0237271 RchiOBHm_Chr7g0237281 RchiOBHm_Chr7g0237311 RchiOBHm_Chr7g0237321
rosa_laevigata	RLG00000001012 RLG00000001018
rosa_multiflora	Rmu_sc0002923.1_g000043 Rmu_sc0002923.1_g000049 Rmu_sc0006438.1_g000001 Rmu_sc0006438.1_g000006 Rmu_sc0006438.1_g000007
rosa_roxburghii	Rroxscaffold_3G00224320
rosa_rugosa	Rorug07G0302600 Rorug07G0302700
rosa_samantha	Rh7AG459300 Rh7AG459500 Rh7AG459600 Rh7AG459700 Rh7AG459800 Rh7BG429700 Rh7CG477100 Rh7DG446400
rosa_wichuraiana	Rw0G003870 Rw7G038060 Rw7G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	519
AccBSI	CCGCTC	1 cut(s)	1447
AccII	CGCG	1 cut(s)	386
AclWI	GGATC	6 cut(s)	135, 671, 1117, 1275, 1655, 1668
AcoI	YGGCCR	1 cut(s)	418
AcsI	RAATTY	2 cut(s)	823, 1202
AcuI	CTGAAG	3 cut(s)	1601, 1734, 1800
AcyI	GRCGYC	2 cut(s)	516, 1380
AfaI	GTAC	7 cut(s)	245, 260, 763, 795, 1335, 1638, 1847
AfiI	CCNNNNNNNGG	4 cut(s)	153, 268, 346, 404
AflII	CTTAAG	2 cut(s)	500, 650
AflIII	ACRYGT	1 cut(s)	1385
AgeI	ACCGGT	1 cut(s)	1698
AhdI	GACNNNNNGTC	1 cut(s)	1241
AjnI	CCWGG	4 cut(s)	345, 1351, 1375, 1570
AloI	GAACNNNNNNTCC	2 cut(s)	326, 358
Alw21I	GWGCWC	4 cut(s)	276, 1265, 1308, 1501
Alw26I	GTCTC	1 cut(s)	166
Alw44I	GTGCAC	1 cut(s)	1304
AlwI	GGATC	6 cut(s)	135, 671, 1117, 1275, 1655, 1668
Ama87I	CYCGRG	3 cut(s)	111, 275, 488
AoxI	GGCC	7 cut(s)	108, 406, 418, 801, 817, 1346, 1647
ApaLI	GTGCAC	1 cut(s)	1304
ApeKI	GCWGC	8 cut(s)	70, 496, 971, 1064, 1411, 1595, 1653, 1754
ApoI	RAATTY	2 cut(s)	823, 1202
ArsI	GACNNNNNNTTYG	2 cut(s)	1385, 1417
AsiGI	ACCGGT	1 cut(s)	1698
Asp700I	GAANNNNTTC	2 cut(s)	80, 803
AspLEI	GCGC	4 cut(s)	48, 232, 386, 496
AspS9I	GGNCC	6 cut(s)	109, 220, 406, 605, 713, 1347
AsuC2I	CCSGG	4 cut(s)	112, 113, 340, 1061
AsuHPI	GGTGA	5 cut(s)	226, 301, 1237, 1403, 1788
AvaI	CYCGRG	3 cut(s)	111, 275, 488
AvaII	GGWCC	3 cut(s)	220, 605, 713
AxyI	CCTNAGG	1 cut(s)	464
BaeGI	GKGCMC	1 cut(s)	1308
BamHI	GGATCC	1 cut(s)	1660
BanII	GRGCYC	3 cut(s)	193, 276, 339
BarI	GAAGNNNNNNTAC	2 cut(s)	1599, 1631
BbsI	GAAGAC	2 cut(s)	185, 953
Bbv12I	GWGCWC	4 cut(s)	276, 1265, 1308, 1501
BbvCI	CCTCAGC	1 cut(s)	621
BbvI	GCAGC	8 cut(s)	57, 483, 983, 1051, 1398, 1607, 1640, 1741
BccI	CCATC	6 cut(s)	179, 550, 888, 1015, 1292, 1572
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	4 cut(s)	347, 1353, 1377, 1572
BclI	TGATCA	1 cut(s)	691
BcnI	CCSGG	4 cut(s)	112, 113, 340, 1061
BcoDI	GTCTC	1 cut(s)	166
BfaI	CTAG	2 cut(s)	153, 903
BfmI	CTRYAG	3 cut(s)	951, 977, 1412
BfoI	RGCGCY	2 cut(s)	49, 497
BfrI	CTTAAG	2 cut(s)	500, 650
BglI	GCCNNNNNGGC	1 cut(s)	556
BglII	AGATCT	1 cut(s)	1487
BmcAI	AGTACT	2 cut(s)	1335, 1638
Bme1390I	CCNGG	8 cut(s)	112, 113, 340, 347, 1061, 1353, 1377, 1572
Bme18I	GGWCC	3 cut(s)	220, 605, 713
BmeRI	GACNNNNNGTC	1 cut(s)	1241
BmeT110I	CYCGRG	3 cut(s)	111, 275, 488
BmgT120I	GGNCC	6 cut(s)	109, 220, 406, 605, 713, 1347
BmiI	GGNNCC	7 cut(s)	192, 222, 336, 344, 431, 1349, 1662
BmrFI	CCNGG	8 cut(s)	112, 113, 340, 347, 1061, 1353, 1377, 1572
BmrI	ACTGGG	1 cut(s)	931
BmsI	GCATC	2 cut(s)	1815, 1828
BmuI	ACTGGG	1 cut(s)	931
BoxI	GACNNNNGTC	1 cut(s)	1701
BpiI	GAAGAC	2 cut(s)	185, 953
BpmI	CTGGAG	2 cut(s)	395, 1620
Bpu10I	CCTNAGC	2 cut(s)	441, 621
BpuEI	CTTGAG	1 cut(s)	837
BpuMI	CCSGG	4 cut(s)	112, 113, 340, 1061
BsaAI	YACGTR	1 cut(s)	1340
BsaHI	GRCGYC	2 cut(s)	516, 1380
BsaI	GGTCTC	1 cut(s)	166
BsaJI	CCNNGG	5 cut(s)	111, 147, 338, 1351, 1819
BsaWI	WCCGGW	1 cut(s)	1698
BsaXI	ACNNNNNCTCC	4 cut(s)	263, 293, 326, 356
Bsc4I	CCNNNNNNNGG	4 cut(s)	153, 268, 346, 404
Bse118I	RCCGGY	2 cut(s)	58, 1698
Bse1I	ACTGG	4 cut(s)	257, 431, 792, 926
Bse21I	CCTNAGG	1 cut(s)	464
Bse3DI	GCAATG	2 cut(s)	1503, 1740
BseBI	CCWGG	4 cut(s)	347, 1353, 1377, 1572
BseDI	CCNNGG	5 cut(s)	111, 147, 338, 1351, 1819
BseGI	GGATG	4 cut(s)	295, 899, 1030, 1843
BseLI	CCNNNNNNNGG	4 cut(s)	153, 268, 346, 404
BseMI	GCAATG	2 cut(s)	1503, 1740
BseMII	CTCAG	5 cut(s)	478, 581, 612, 708, 1283
BseNI	ACTGG	4 cut(s)	257, 431, 792, 926
BseRI	GAGGAG	2 cut(s)	383, 636
BseSI	GKGCMC	1 cut(s)	1308
BseXI	GCAGC	8 cut(s)	57, 483, 983, 1051, 1398, 1607, 1640, 1741
BseYI	CCCAGC	1 cut(s)	1583
Bsh1236I	CGCG	1 cut(s)	386
BshFI	GGCC	7 cut(s)	110, 408, 420, 803, 819, 1348, 1649
BshTI	ACCGGT	1 cut(s)	1698
BsiHKAI	GWGCWC	4 cut(s)	276, 1265, 1308, 1501
BsiHKCI	CYCGRG	3 cut(s)	111, 275, 488
BsiSI	CCGG	9 cut(s)	15, 59, 107, 112, 340, 551, 968, 1061, 1699
BslI	CCNNNNNNNGG	4 cut(s)	153, 268, 346, 404
BsmAI	GTCTC	1 cut(s)	166
BsmI	GAATGC	1 cut(s)	1413
BsnI	GGCC	7 cut(s)	110, 408, 420, 803, 819, 1348, 1649
Bso31I	GGTCTC	1 cut(s)	166
BsoBI	CYCGRG	3 cut(s)	111, 275, 488
Bsp1286I	GDGCHC	6 cut(s)	193, 276, 339, 1265, 1308, 1501
BspANI	GGCC	7 cut(s)	110, 408, 420, 803, 819, 1348, 1649
BspCNI	CTCAG	5 cut(s)	477, 580, 613, 709, 1284
BspFNI	CGCG	1 cut(s)	386
BspHI	TCATGA	1 cut(s)	1357
BspLI	GGNNCC	7 cut(s)	192, 222, 336, 344, 431, 1349, 1662
BspMAI	CTGCAG	3 cut(s)	955, 981, 1416
BspPI	GGATC	6 cut(s)	135, 671, 1117, 1275, 1655, 1668
BspTI	CTTAAG	2 cut(s)	500, 650
BspTNI	GGTCTC	1 cut(s)	166
BsrBI	CCGCTC	1 cut(s)	1447
BsrDI	GCAATG	2 cut(s)	1503, 1740
BsrFI	RCCGGY	2 cut(s)	58, 1698
BsrI	ACTGG	4 cut(s)	257, 431, 792, 926
BssAI	RCCGGY	2 cut(s)	58, 1698
BssECI	CCNNGG	5 cut(s)	111, 147, 338, 1351, 1819
BssNI	GRCGYC	2 cut(s)	516, 1380
Bst2UI	CCWGG	4 cut(s)	347, 1353, 1377, 1572
Bst4CI	ACNGT	8 cut(s)	612, 1045, 1103, 1243, 1462, 1468, 1674, 1850
BstACI	GRCGYC	2 cut(s)	516, 1380
BstAFI	CTTAAG	2 cut(s)	500, 650
BstBAI	YACGTR	1 cut(s)	1340
BstC8I	GCNNGC	9 cut(s)	50, 228, 447, 817, 1005, 1009, 1074, 1275, 1651
BstDEI	CTNAG	7 cut(s)	441, 464, 567, 621, 717, 1049, 1292
BstDSI	CCRYGG	1 cut(s)	147
BstF5I	GGATG	4 cut(s)	295, 899, 1030, 1843
BstFNI	CGCG	1 cut(s)	386
BstH2I	RGCGCY	2 cut(s)	49, 497
BstHHI	GCGC	4 cut(s)	48, 232, 386, 496
BstMAI	GTCTC	1 cut(s)	166
BstNI	CCWGG	4 cut(s)	347, 1353, 1377, 1572
BstNSI	RCATGY	3 cut(s)	230, 1277, 1389
BstPAI	GACNNNNGTC	1 cut(s)	1701
BstSCI	CCNGG	8 cut(s)	110, 111, 338, 345, 1059, 1351, 1375, 1570
BstSFI	CTRYAG	3 cut(s)	951, 977, 1412
BstSLI	GKGCMC	1 cut(s)	1308
BstUI	CGCG	1 cut(s)	386
BstV1I	GCAGC	8 cut(s)	57, 483, 983, 1051, 1398, 1607, 1640, 1741
BstV2I	GAAGAC	2 cut(s)	185, 953
BstX2I	RGATCY	2 cut(s)	1487, 1660
BstXI	CCANNNNNNTGG	1 cut(s)	1571
BstYI	RGATCY	2 cut(s)	1487, 1660
Bsu36I	CCTNAGG	1 cut(s)	464
BsuRI	GGCC	7 cut(s)	110, 408, 420, 803, 819, 1348, 1649
BtgI	CCRYGG	1 cut(s)	147
BtgZI	GCGATG	1 cut(s)	1759
BtsCI	GGATG	4 cut(s)	295, 899, 1030, 1843
BtsI	GCAGTG	1 cut(s)	974
BtsIMutI	CAGTG	3 cut(s)	974, 1041, 1473
Cac8I	GCNNGC	9 cut(s)	50, 228, 447, 817, 1005, 1009, 1074, 1275, 1651
CciI	TCATGA	1 cut(s)	1357
CfoI	GCGC	4 cut(s)	48, 232, 386, 496
Cfr10I	RCCGGY	2 cut(s)	58, 1698
Cfr13I	GGNCC	6 cut(s)	109, 220, 406, 605, 713, 1347
Cfr9I	CCCGGG	1 cut(s)	111
CseI	GACGC	2 cut(s)	1369, 1687
CsiI	ACCWGGT	1 cut(s)	1570
Csp6I	GTAC	7 cut(s)	244, 259, 762, 794, 1334, 1637, 1846
CspAI	ACCGGT	1 cut(s)	1698
CspCI	CAANNNNNGTGG	2 cut(s)	1473, 1508
CviAII	CATG	9 cut(s)	227, 412, 913, 1183, 1274, 1358, 1386, 1706, 1750
CviQI	GTAC	7 cut(s)	244, 259, 762, 794, 1334, 1637, 1846
DdeI	CTNAG	7 cut(s)	441, 464, 567, 621, 717, 1049, 1292
DriI	GACNNNNNGTC	1 cut(s)	1241
EaeI	YGGCCR	1 cut(s)	418
Eam1105I	GACNNNNNGTC	1 cut(s)	1241
EciI	GGCGGA	4 cut(s)	140, 182, 253, 1026
Ecl136II	GAGCTC	1 cut(s)	274
Eco147I	AGGCCT	1 cut(s)	803
Eco24I	GRGCYC	3 cut(s)	193, 276, 339
Eco31I	GGTCTC	1 cut(s)	166
Eco47I	GGWCC	3 cut(s)	220, 605, 713
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	3 cut(s)	1601, 1734, 1800
Eco81I	CCTNAGG	1 cut(s)	464
Eco88I	CYCGRG	3 cut(s)	111, 275, 488
EcoICRI	GAGCTC	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	220, 1347
EcoRII	CCWGG	4 cut(s)	345, 1351, 1375, 1570
EcoT38I	GRGCYC	3 cut(s)	193, 276, 339
FaeI	CATG	9 cut(s)	230, 415, 916, 1186, 1277, 1361, 1389, 1709, 1753
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FatI	CATG	9 cut(s)	226, 411, 912, 1182, 1273, 1357, 1385, 1705, 1749
FauI	CCCGC	4 cut(s)	231, 676, 1440, 1617
FbaI	TGATCA	1 cut(s)	691
FokI	GGATG	4 cut(s)	302, 906, 1037, 1850
FriOI	GRGCYC	3 cut(s)	193, 276, 339
FspBI	CTAG	2 cut(s)	153, 903
GlaI	GCGC	4 cut(s)	47, 231, 385, 495
GsaI	CCCAGC	1 cut(s)	1587
GsuI	CTGGAG	2 cut(s)	395, 1620
HaeII	RGCGCY	2 cut(s)	49, 497
HaeIII	GGCC	7 cut(s)	110, 408, 420, 803, 819, 1348, 1649
HapII	CCGG	9 cut(s)	15, 59, 107, 112, 340, 551, 968, 1061, 1699
HgaI	GACGC	2 cut(s)	1369, 1687
HhaI	GCGC	4 cut(s)	48, 232, 386, 496
Hin1I	GRCGYC	2 cut(s)	516, 1380
Hin1II	CATG	9 cut(s)	230, 415, 916, 1186, 1277, 1361, 1389, 1709, 1753
Hin6I	GCGC	4 cut(s)	46, 230, 384, 494
HinP1I	GCGC	4 cut(s)	46, 230, 384, 494
HincII	GTYRAC	2 cut(s)	250, 1677
HindII	GTYRAC	2 cut(s)	250, 1677
HindIII	AAGCTT	1 cut(s)	443
HinfI	GANTC	5 cut(s)	295, 906, 1187, 1228, 1451
HpaII	CCGG	9 cut(s)	15, 59, 107, 112, 340, 551, 968, 1061, 1699
HphI	GGTGA	5 cut(s)	226, 301, 1237, 1403, 1788
Hpy166II	GTNNAC	7 cut(s)	250, 309, 564, 1306, 1549, 1677, 1796
Hpy188I	TCNGA	8 cut(s)	36, 195, 328, 699, 943, 1099, 1620, 1823
Hpy188III	TCNNGA	8 cut(s)	287, 398, 635, 716, 767, 854, 1358, 1528
Hpy8I	GTNNAC	7 cut(s)	250, 309, 564, 1306, 1549, 1677, 1796
Hpy99I	CGWCG	4 cut(s)	40, 317, 518, 521
HpyCH4III	ACNGT	8 cut(s)	612, 1045, 1103, 1243, 1462, 1468, 1674, 1850
HpyCH4IV	ACGT	4 cut(s)	246, 516, 1339, 1833
HpyF3I	CTNAG	7 cut(s)	441, 464, 567, 621, 717, 1049, 1292
HpySE526I	ACGT	4 cut(s)	246, 516, 1339, 1833
Hsp92I	GRCGYC	2 cut(s)	516, 1380
Hsp92II	CATG	9 cut(s)	230, 415, 916, 1186, 1277, 1361, 1389, 1709, 1753
HspAI	GCGC	4 cut(s)	46, 230, 384, 494
Ksp22I	TGATCA	1 cut(s)	691
LmnI	GCTCC	9 cut(s)	196, 208, 271, 334, 370, 435, 637, 1260, 1366
Lsp1109I	GCAGC	8 cut(s)	57, 483, 983, 1051, 1398, 1607, 1640, 1741
LweI	GCATC	2 cut(s)	1815, 1828
MabI	ACCWGGT	1 cut(s)	1570
MaeI	CTAG	2 cut(s)	153, 903
MaeII	ACGT	4 cut(s)	246, 516, 1339, 1833
MaeIII	GTNAC	6 cut(s)	783, 1318, 1381, 1391, 1462, 1477
MbiI	CCGCTC	1 cut(s)	1447
MboII	GAAGA	8 cut(s)	190, 537, 953, 956, 1121, 1484, 1697, 1727
MflI	RGATCY	2 cut(s)	1487, 1660
MhlI	GDGCHC	6 cut(s)	193, 276, 339, 1265, 1308, 1501
MluCI	AATT	8 cut(s)	585, 687, 823, 885, 984, 1202, 1309, 1455
MlyI	GAGTC	2 cut(s)	304, 1460
MmeI	TCCRAC	2 cut(s)	59, 562
MroXI	GAANNNNTTC	2 cut(s)	80, 803
MseI	TTAA	7 cut(s)	501, 534, 651, 993, 1212, 1554, 1852
MspA1I	CMGCKG	6 cut(s)	52, 525, 683, 1177, 1598, 1653
MspCI	CTTAAG	2 cut(s)	500, 650
MspI	CCGG	9 cut(s)	15, 59, 107, 112, 340, 551, 968, 1061, 1699
MspR9I	CCNGG	8 cut(s)	112, 113, 340, 347, 1061, 1353, 1377, 1572
Mva1269I	GAATGC	1 cut(s)	1413
MvaI	CCWGG	4 cut(s)	347, 1353, 1377, 1572
MvnI	CGCG	1 cut(s)	386
NciI	CCSGG	4 cut(s)	112, 113, 340, 1061
NlaIII	CATG	9 cut(s)	230, 415, 916, 1186, 1277, 1361, 1389, 1709, 1753
NlaIV	GGNNCC	7 cut(s)	192, 222, 336, 344, 431, 1349, 1662
NmeAIII	GCCGAG	1 cut(s)	143
NmuCI	GTSAC	4 cut(s)	1318, 1381, 1391, 1477
NspI	RCATGY	3 cut(s)	230, 1277, 1389
PaeI	GCATGC	2 cut(s)	230, 1277
PaeR7I	CTCGAG	1 cut(s)	488
PagI	TCATGA	1 cut(s)	1357
PceI	AGGCCT	1 cut(s)	803
PciI	ACATGT	1 cut(s)	1385
PcsI	WCGNNNNNNNCGW	1 cut(s)	309
PctI	GAATGC	1 cut(s)	1413
PdmI	GAANNNNTTC	2 cut(s)	80, 803
PfeI	GAWTC	3 cut(s)	906, 1187, 1228
PflFI	GACNNNGTC	1 cut(s)	518
PinAI	ACCGGT	1 cut(s)	1698
PleI	GAGTC	2 cut(s)	303, 1459
PpsI	GAGTC	2 cut(s)	303, 1459
Ppu21I	YACGTR	1 cut(s)	1340
PpuMI	RGGWCCY	1 cut(s)	220
PscI	ACATGT	1 cut(s)	1385
PshAI	GACNNNNGTC	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	220
Psp6I	CCWGG	4 cut(s)	345, 1351, 1375, 1570
PspFI	CCCAGC	1 cut(s)	1583
PspGI	CCWGG	4 cut(s)	345, 1351, 1375, 1570
PspN4I	GGNNCC	7 cut(s)	192, 222, 336, 344, 431, 1349, 1662
PspPI	GGNCC	6 cut(s)	109, 220, 406, 605, 713, 1347
PspPPI	RGGWCCY	1 cut(s)	220
PspXI	VCTCGAGB	1 cut(s)	488
PstI	CTGCAG	3 cut(s)	955, 981, 1416
PsuI	RGATCY	2 cut(s)	1487, 1660
PsyI	GACNNNGTC	1 cut(s)	518
PvuII	CAGCTG	4 cut(s)	52, 1177, 1598, 1653
RsaI	GTAC	7 cut(s)	245, 260, 763, 795, 1335, 1638, 1847
RsaNI	GTAC	7 cut(s)	244, 259, 762, 794, 1334, 1637, 1846
SacI	GAGCTC	1 cut(s)	276
SaqAI	TTAA	7 cut(s)	501, 534, 651, 993, 1212, 1554, 1852
Sau96I	GGNCC	6 cut(s)	109, 220, 406, 605, 713, 1347
ScaI	AGTACT	2 cut(s)	1335, 1638
SchI	GAGTC	2 cut(s)	304, 1460
ScrFI	CCNGG	8 cut(s)	112, 113, 340, 347, 1061, 1353, 1377, 1572
SduI	GDGCHC	6 cut(s)	193, 276, 339, 1265, 1308, 1501
SexAI	ACCWGGT	1 cut(s)	1570
SfaNI	GCATC	2 cut(s)	1815, 1828
SfcI	CTRYAG	3 cut(s)	951, 977, 1412
Sfr274I	CTCGAG	1 cut(s)	488
SinI	GGWCC	3 cut(s)	220, 605, 713
SlaI	CTCGAG	1 cut(s)	488
SmaI	CCCGGG	1 cut(s)	113
SmlI	CTYRAG	4 cut(s)	488, 500, 650, 852
SmoI	CTYRAG	4 cut(s)	488, 500, 650, 852
SphI	GCATGC	2 cut(s)	230, 1277
Sse9I	AATT	8 cut(s)	585, 687, 823, 885, 984, 1202, 1309, 1455
SseBI	AGGCCT	1 cut(s)	803
SspMI	CTAG	2 cut(s)	153, 903
SstI	GAGCTC	1 cut(s)	276
StuI	AGGCCT	1 cut(s)	803
StyD4I	CCNGG	8 cut(s)	110, 111, 338, 345, 1059, 1351, 1375, 1570
TaaI	ACNGT	8 cut(s)	612, 1045, 1103, 1243, 1462, 1468, 1674, 1850
TaiI	ACGT	4 cut(s)	249, 519, 1342, 1836
TaqI	TCGA	2 cut(s)	489, 1329
TaqII	GACCGA	1 cut(s)	197
TasI	AATT	8 cut(s)	585, 687, 823, 885, 984, 1202, 1309, 1455
TatI	WGTACW	3 cut(s)	793, 1333, 1636
TauI	GCSGC	3 cut(s)	353, 411, 541
TfiI	GAWTC	3 cut(s)	906, 1187, 1228
Tru1I	TTAA	7 cut(s)	501, 534, 651, 993, 1212, 1554, 1852
Tru9I	TTAA	7 cut(s)	501, 534, 651, 993, 1212, 1554, 1852
TscAI	CASTG	3 cut(s)	981, 1048, 1473
TseFI	GTSAC	4 cut(s)	1318, 1381, 1391, 1477
TseI	GCWGC	8 cut(s)	70, 496, 971, 1064, 1411, 1595, 1653, 1754
Tsp45I	GTSAC	4 cut(s)	1318, 1381, 1391, 1477
TspDTI	ATGAA	2 cut(s)	218, 1315
TspGWI	ACGGA	4 cut(s)	136, 422, 797, 1280
TspMI	CCCGGG	1 cut(s)	111
TspRI	CASTG	3 cut(s)	981, 1048, 1473
Tth111I	GACNNNGTC	1 cut(s)	518
Vha464I	CTTAAG	2 cut(s)	500, 650
VneI	GTGCAC	1 cut(s)	1304
VpaK11BI	GGWCC	3 cut(s)	220, 605, 713
XapI	RAATTY	2 cut(s)	823, 1202
XceI	RCATGY	3 cut(s)	230, 1277, 1389
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1359
XhoI	CTCGAG	1 cut(s)	488
XmaI	CCCGGG	1 cut(s)	111
XmnI	GAANNNNTTC	2 cut(s)	80, 803
XspI	CTAG	2 cut(s)	153, 903
ZraI	GACGTC	1 cut(s)	517
ZrmI	AGTACT	2 cut(s)	1335, 1638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.