Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003547

Physical Location & Seq

Genomic Coordinates Reverse (-)

62323660 .. 62337880

Gene Structure 14221 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21269

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGGCTTCAATTTCCGGCATTTATTCCTCTTCTCCGACTCTGCAACCGCGGCTGAGACCGGCGGCGAGTCTATTACGTAAAGACTCCGTTTCGTTTCCGACGGCCGGGCCCAGGCCCGGCGATCGGAGCTTGTTCGCTCAGTCGGTGGCGCGGGAGATCAAGGCCGAGTTGGCAAAGGTTGACGGCGATGGCGCTGTGATTAAGGAGAAGAAGAAGAAGGTTCTGGAGAAGGATCCTCGGGCGTTGTGGCGGAGGTACGTGGACTGGTTGTACCAGCACAAGGAGCTGGGGCTATATTTGGATGTGAGTCGGGTCGGGTTTACCGACGAGTTCGTCGCGGAAATGGAGCCCCGGCTCCAGGCGGCGTTTAAGGCGATGGATGACCTGGAGAAAGGCGCGATTGCGAATCCCGATGAGGGGCGGATGGTCGGGCATTACTGGCTGAGAGACCCTAAGCTGGCGCCGAAACCGATACTCACGGTTCAGATTGAGAACACGCTCGAGGCGCTGCTGAAGTTCTCCGGCGACGTCGTCAGCGGTAAGATTAAGCCGCCGGCTTCTTCGGAGGGTCGTTTTACTCAGGTTCTTTCGGTTGGAATCGGAGGCTCGGCACTTGGACCACAGTTTGTTGCTGAGGCATTGGCGCCTGATGTTCCTCCTCTTAAGATACGATTCATTGACAATACCGATCCAGCAGGAATTGATCATCAGATTGCACAGCTCGGTGAAGAGCTGGCTTCTACTCTTGTAATTGTGATTTCAAAGAGTGGAGGTACTCCGGAAACTAGAAATGGTTTACTCGAAGTACAGAAGGCCTTTCGTGAGGCTGGCCTAAATTTCGCAAAACAGGGAGTGGCTATCACTCAAGAAAATTCATTATTAGACAACACTGCTAGAATTGAGGGCTGGTTAGCTAGATTCCCAATGTTTGACTGGGTTGGTGGAAGAACATCTGAAATGTCTGCAGTTGGTCTTCTTCCAGCAGCGCTTCAGGGAATTGACATTAAAGAAATGCTCGCTGGTGGAAAGTTGATGGATGAAGCTAATAGAACCACTGTGGTTAAGAATAATCCTGCCGCCTTGCTTGCTTTATGCTGGTATTGGGCTACTGATGGATTGGGATCCAAGGATATGGTTGTTCTTCCTTACAAGGACAGCCTATTATTATTTAGTCGGTATTTGCAGCAGTTGGTCATGGAATCTATTGGGAAAGAATTTGACCTTGATGGTAATCGGGTGAATCAAGGACTTACAGTGTACGGAAACAAAGGGAGCACGGATCAGCATGCATACATCCAACAACTGAGAGATGGCGTGCACAATTTCTTTGTGACATTCATTGAAGTACTACGAGATAGGCCTCCTGGTCATGATTGGGAGCTTGAACCAGGTGTCACATGTGGTGACTATCTATTTGGAATGCTACAGGGAACACGATCGGCTCTTTATTCTAATGACCGAGAGTCGATTACGGTTTCTGTGGAAGAAGTGACACCTAGATCTGTAGGTGCTCTTATAGCACTTTATGAGAGAGCGGTTGGGATTTATGCCATACTTGTCAACATTAATGCTTACCATCAACCTGGTGTGGAGGCCGGGAAGAAAGCAGCCGGAGAAGTATTAGCTCTTCAGAAACGGGTTTTATCCGTACTTAATGAGGCCAGCTGTAAAGAGCCTGTTGAGCCATTGACACTAGATGAAGTAGCCGATCGGTGCCACGCTGCTGAAGATATTGAAATAATTTACAAGATCATCGCTCACATGGCGGCAAATGACAGAGCTCTTATTGCTGAAGGCAGTTGCGGTTCACCACGGAGTGTCAAAGTTTTCCTCGGCGAGTGTAATGTGGAGGCATTATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009909 GO:0009911 GO:0009941 GO:0009987 GO:0016853 GO:0016860 GO:0016861 GO:0031967 GO:0031975 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PGI

Protein Analysis

620

Amino Acids

67.75

Weight (kDa)

5.47

Isoelectric Point (pI)

36.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGI	PF00342	125 - 451	4.6e-47	Phosphoglucose isomerase
PGI	PF00342	482 - 542	4.9e-10	Phosphoglucose isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24620 AT4G24620
fragaria_vesca	FvH4_5g35680 FvH4_5g35680
malus_domestica	MD08G1194100.v1.1 MD15G1382200.v1.1
prunus_persica	Prupe.1G528400_v2.0.a1 Prupe.1G528400_v2.0.a1
pyrus_communis	pycom15g34220
rosa_chinensis	RchiOBHm_Chr7g0237251 RchiOBHm_Chr7g0237271 RchiOBHm_Chr7g0237281 RchiOBHm_Chr7g0237311 RchiOBHm_Chr7g0237321
rosa_laevigata	RLG00000001012 RLG00000001018
rosa_multiflora	Rmu_sc0002923.1_g000043 Rmu_sc0002923.1_g000049 Rmu_sc0006438.1_g000001 Rmu_sc0006438.1_g000006 Rmu_sc0006438.1_g000007
rosa_roxburghii	Rroxscaffold_3G00224320
rosa_rugosa	Rorug07G0302600 Rorug07G0302700
rosa_samantha	Rh7AG459300 Rh7AG459500 Rh7AG459600 Rh7AG459700 Rh7AG459800 Rh7BG429700 Rh7CG477100 Rh7DG446400
rosa_wichuraiana	Rw0G003870 Rw7G038060 Rw7G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	332
AatII	GACGTC	1 cut(s)	531
AccB1I	GGYRCC	3 cut(s)	460, 643, 1713
AccBSI	CCGCTC	1 cut(s)	1535
AccII	CGCG	4 cut(s)	49, 151, 338, 398
AccIII	TCCGGA	1 cut(s)	778
AclWI	GGATC	6 cut(s)	227, 240, 683, 1116, 1129, 1287
AcoI	YGGCCR	1 cut(s)	102
AcsI	RAATTY	3 cut(s)	835, 871, 1214
AcuI	CTGAAG	5 cut(s)	533, 974, 1613, 1746, 1812
AcyI	GRCGYC	3 cut(s)	461, 528, 644
AdeI	CACNNNGTG	1 cut(s)	1817
AfaI	GTAC	7 cut(s)	257, 272, 775, 807, 1259, 1347, 1650
AfeI	AGCGCT	1 cut(s)	987
AfiI	CCNNNNNNNGG	9 cut(s)	104, 111, 116, 123, 280, 358, 416, 417, 457
AflII	CTTAAG	1 cut(s)	662
AflIII	ACRYGT	1 cut(s)	1397
AgsI	TTSAA	5 cut(s)	9, 762, 1343, 1385, 1736
AhdI	GACNNNNNGTC	1 cut(s)	1462
AjnI	CCWGG	6 cut(s)	110, 357, 384, 1363, 1387, 1582
Alw21I	GWGCWC	4 cut(s)	1277, 1320, 1513, 1783
Alw26I	GTCTC	2 cut(s)	49, 441
Alw44I	GTGCAC	1 cut(s)	1316
AlwI	GGATC	6 cut(s)	227, 240, 683, 1116, 1129, 1287
Ama87I	CYCGRG	2 cut(s)	237, 500
Aor13HI	TCCGGA	1 cut(s)	778
Aor51HI	AGCGCT	1 cut(s)	987
AoxI	GGCC	9 cut(s)	102, 107, 113, 162, 813, 829, 1358, 1593, 1659
ApaI	GGGCCC	1 cut(s)	111
ApaLI	GTGCAC	1 cut(s)	1316
ApeKI	GCWGC	5 cut(s)	508, 983, 1183, 1607, 1721
ApoI	RAATTY	3 cut(s)	835, 871, 1214
ArsI	GACNNNNNNTTYG	2 cut(s)	1397, 1429
AseI	ATTAAT	1 cut(s)	1566
AspLEI	GCGC	7 cut(s)	151, 194, 398, 463, 508, 646, 988
AspS9I	GGNCC	4 cut(s)	107, 108, 114, 617
AsuC2I	CCSGG	4 cut(s)	106, 117, 352, 1597
AsuHPI	GGTGA	4 cut(s)	737, 1249, 1415, 1800
AvaI	CYCGRG	2 cut(s)	237, 500
AvaII	GGWCC	1 cut(s)	617
BaeGI	GKGCMC	2 cut(s)	111, 1320
BamHI	GGATCC	2 cut(s)	232, 1121
BanI	GGYRCC	3 cut(s)	460, 643, 1713
BanII	GRGCYC	3 cut(s)	111, 351, 1783
BarI	GAAGNNNNNNTAC	2 cut(s)	1611, 1643
BbsI	GAAGAC	1 cut(s)	965
Bbv12I	GWGCWC	4 cut(s)	1277, 1320, 1513, 1783
BbvCI	CCTCAGC	1 cut(s)	633
BbvI	GCAGC	5 cut(s)	495, 995, 1195, 1619, 1708
BccI	CCATC	8 cut(s)	182, 370, 418, 1027, 1106, 1220, 1304, 1584
BceAI	ACGGC	2 cut(s)	117, 199
BciT130I	CCWGG	6 cut(s)	112, 359, 386, 1365, 1389, 1584
BclI	TGATCA	1 cut(s)	703
BcnI	CCSGG	4 cut(s)	106, 117, 352, 1597
BcoDI	GTCTC	2 cut(s)	49, 441
BfaI	CTAG	5 cut(s)	786, 894, 915, 1497, 1694
BfmI	CTRYAG	3 cut(s)	963, 1424, 1503
BfoI	RGCGCY	5 cut(s)	195, 464, 509, 647, 989
BfrI	CTTAAG	1 cut(s)	662
BglI	GCCNNNNNGGC	1 cut(s)	170
BglII	AGATCT	1 cut(s)	1499
BmcAI	AGTACT	1 cut(s)	1347
Bme18I	GGWCC	1 cut(s)	617
BmeRI	GACNNNNNGTC	1 cut(s)	1462
BmeT110I	CYCGRG	2 cut(s)	237, 500
BmgT120I	GGNCC	4 cut(s)	107, 108, 114, 617
BmiI	GGNNCC	8 cut(s)	109, 234, 348, 356, 462, 645, 1123, 1715
BmrI	ACTGGG	1 cut(s)	943
BmuI	ACTGGG	1 cut(s)	943
BpiI	GAAGAC	1 cut(s)	965
BpmI	CTGGAG	3 cut(s)	245, 341, 407
Bpu10I	CCTNAGC	2 cut(s)	453, 633
BpuEI	CTTGAG	1 cut(s)	849
BpuMI	CCSGG	4 cut(s)	106, 117, 352, 1597
BsaAI	YACGTR	2 cut(s)	77, 259
BsaBI	GATNNNNATC	2 cut(s)	1120, 1230
BsaHI	GRCGYC	3 cut(s)	461, 528, 644
BsaI	GGTCTC	2 cut(s)	49, 441
BsaJI	CCNNGG	7 cut(s)	47, 110, 236, 350, 1125, 1811, 1831
BsaWI	WCCGGW	1 cut(s)	778
Bsc4I	CCNNNNNNNGG	9 cut(s)	104, 111, 116, 123, 280, 358, 416, 417, 457
Bse118I	RCCGGY	2 cut(s)	58, 553
Bse1I	ACTGG	3 cut(s)	269, 443, 938
Bse8I	GATNNNNATC	2 cut(s)	1120, 1230
BseAI	TCCGGA	1 cut(s)	778
BseBI	CCWGG	6 cut(s)	112, 359, 386, 1365, 1389, 1584
BseDI	CCNNGG	7 cut(s)	47, 110, 236, 350, 1125, 1811, 1831
BseGI	GGATG	5 cut(s)	307, 385, 429, 1042, 1293
BseJI	GATNNNNATC	2 cut(s)	1120, 1230
BseLI	CCNNNNNNNGG	9 cut(s)	104, 111, 116, 123, 280, 358, 416, 417, 457
BseMII	CTCAG	6 cut(s)	44, 152, 434, 593, 624, 1295
BseNI	ACTGG	3 cut(s)	269, 443, 938
BseRI	GAGGAG	1 cut(s)	648
BseSI	GKGCMC	2 cut(s)	111, 1320
BseX3I	CGGCCG	1 cut(s)	102
BseXI	GCAGC	5 cut(s)	495, 995, 1195, 1619, 1708
BseYI	CCCAGC	1 cut(s)	286
Bsh1236I	CGCG	4 cut(s)	49, 151, 338, 398
Bsh1285I	CGRYCG	4 cut(s)	105, 124, 1439, 1711
BshFI	GGCC	9 cut(s)	104, 109, 115, 164, 815, 831, 1360, 1595, 1661
BshNI	GGYRCC	3 cut(s)	460, 643, 1713
BsiEI	CGRYCG	4 cut(s)	105, 124, 1439, 1711
BsiHKAI	GWGCWC	4 cut(s)	1277, 1320, 1513, 1783
BsiHKCI	CYCGRG	2 cut(s)	237, 500
BslI	CCNNNNNNNGG	9 cut(s)	104, 111, 116, 123, 280, 358, 416, 417, 457
BsmAI	GTCTC	2 cut(s)	49, 441
BsmI	GAATGC	1 cut(s)	1425
BsnI	GGCC	9 cut(s)	104, 109, 115, 164, 815, 831, 1360, 1595, 1661
Bso31I	GGTCTC	2 cut(s)	49, 441
BsoBI	CYCGRG	2 cut(s)	237, 500
Bsp120I	GGGCCC	1 cut(s)	107
Bsp1286I	GDGCHC	6 cut(s)	111, 351, 1277, 1320, 1513, 1783
Bsp13I	TCCGGA	1 cut(s)	778
BspANI	GGCC	9 cut(s)	104, 109, 115, 164, 815, 831, 1360, 1595, 1661
BspCNI	CTCAG	6 cut(s)	45, 151, 435, 592, 625, 1296
BspEI	TCCGGA	1 cut(s)	778
BspFNI	CGCG	4 cut(s)	49, 151, 338, 398
BspHI	TCATGA	1 cut(s)	1369
BspLI	GGNNCC	8 cut(s)	109, 234, 348, 356, 462, 645, 1123, 1715
BspMAI	CTGCAG	1 cut(s)	967
BspPI	GGATC	6 cut(s)	227, 240, 683, 1116, 1129, 1287
BspQI	GCTCTTC	2 cut(s)	723, 1632
BspT107I	GGYRCC	3 cut(s)	460, 643, 1713
BspTI	CTTAAG	1 cut(s)	662
BspTNI	GGTCTC	2 cut(s)	49, 441
BsrBI	CCGCTC	1 cut(s)	1535
BsrFI	RCCGGY	2 cut(s)	58, 553
BsrI	ACTGG	3 cut(s)	269, 443, 938
BssAI	RCCGGY	2 cut(s)	58, 553
BssECI	CCNNGG	7 cut(s)	47, 110, 236, 350, 1125, 1811, 1831
BssNI	GRCGYC	3 cut(s)	461, 528, 644
BssT1I	CCWWGG	1 cut(s)	1125
Bst2UI	CCWGG	6 cut(s)	112, 359, 386, 1365, 1389, 1584
Bst4CI	ACNGT	5 cut(s)	481, 624, 1057, 1255, 1474
Bst6I	CTCTTC	3 cut(s)	34, 723, 1632
BstACI	GRCGYC	3 cut(s)	461, 528, 644
BstAFI	CTTAAG	1 cut(s)	662
BstBAI	YACGTR	2 cut(s)	77, 259
BstC8I	GCNNGC	9 cut(s)	459, 555, 735, 829, 1017, 1086, 1287, 1316, 1663
BstDEI	CTNAG	7 cut(s)	53, 138, 443, 453, 579, 633, 1304
BstDSI	CCRYGG	2 cut(s)	47, 1811
BstF5I	GGATG	5 cut(s)	307, 385, 429, 1042, 1293
BstFNI	CGCG	4 cut(s)	49, 151, 338, 398
BstH2I	RGCGCY	5 cut(s)	195, 464, 509, 647, 989
BstHHI	GCGC	7 cut(s)	151, 194, 398, 463, 508, 646, 988
BstMAI	GTCTC	2 cut(s)	49, 441
BstMCI	CGRYCG	4 cut(s)	105, 124, 1439, 1711
BstNI	CCWGG	6 cut(s)	112, 359, 386, 1365, 1389, 1584
BstNSI	RCATGY	2 cut(s)	1289, 1401
BstSFI	CTRYAG	3 cut(s)	963, 1424, 1503
BstSLI	GKGCMC	2 cut(s)	111, 1320
BstSNI	TACGTA	1 cut(s)	77
BstUI	CGCG	4 cut(s)	49, 151, 338, 398
BstV1I	GCAGC	5 cut(s)	495, 995, 1195, 1619, 1708
BstV2I	GAAGAC	1 cut(s)	965
BstX2I	RGATCY	3 cut(s)	232, 1121, 1499
BstXI	CCANNNNNNTGG	2 cut(s)	1132, 1583
BstYI	RGATCY	3 cut(s)	232, 1121, 1499
BstZI	CGGCCG	1 cut(s)	102
BsuRI	GGCC	9 cut(s)	104, 109, 115, 164, 815, 831, 1360, 1595, 1661
BtgI	CCRYGG	2 cut(s)	47, 1811
BtgZI	GCGATG	3 cut(s)	201, 389, 1738
BtsCI	GGATG	5 cut(s)	307, 385, 429, 1042, 1293
BtsI	GCAGTG	1 cut(s)	888
BtsIMutI	CAGTG	3 cut(s)	888, 1053, 1260
Cac8I	GCNNGC	9 cut(s)	459, 555, 735, 829, 1017, 1086, 1287, 1316, 1663
CciI	TCATGA	1 cut(s)	1369
CfoI	GCGC	7 cut(s)	151, 194, 398, 463, 508, 646, 988
Cfr10I	RCCGGY	2 cut(s)	58, 553
Cfr13I	GGNCC	4 cut(s)	107, 108, 114, 617
Cfr42I	CCGCGG	1 cut(s)	50
CsiI	ACCWGGT	2 cut(s)	1387, 1582
Csp6I	GTAC	7 cut(s)	256, 271, 774, 806, 1258, 1346, 1649
CviAII	CATG	5 cut(s)	1195, 1286, 1370, 1398, 1762
CviQI	GTAC	7 cut(s)	256, 271, 774, 806, 1258, 1346, 1649
DdeI	CTNAG	7 cut(s)	53, 138, 443, 453, 579, 633, 1304
DinI	GGCGCC	2 cut(s)	462, 645
DraIII	CACNNNGTG	1 cut(s)	1817
DrdI	GACNNNNNNGTC	1 cut(s)	332
DriI	GACNNNNNGTC	1 cut(s)	1462
DseDI	GACNNNNNNGTC	1 cut(s)	332
EaeI	YGGCCR	1 cut(s)	102
EagI	CGGCCG	1 cut(s)	102
Eam1104I	CTCTTC	3 cut(s)	34, 723, 1632
Eam1105I	GACNNNNNGTC	1 cut(s)	1462
EarI	CTCTTC	3 cut(s)	34, 723, 1632
EciI	GGCGGA	2 cut(s)	265, 436
Ecl136II	GAGCTC	1 cut(s)	1781
EclXI	CGGCCG	1 cut(s)	102
Eco105I	TACGTA	1 cut(s)	77
Eco130I	CCWWGG	1 cut(s)	1125
Eco147I	AGGCCT	2 cut(s)	815, 1360
Eco24I	GRGCYC	3 cut(s)	111, 351, 1783
Eco31I	GGTCTC	2 cut(s)	49, 441
Eco47I	GGWCC	1 cut(s)	617
Eco47III	AGCGCT	1 cut(s)	987
Eco52I	CGGCCG	1 cut(s)	102
Eco53kI	GAGCTC	1 cut(s)	1781
Eco57I	CTGAAG	5 cut(s)	533, 974, 1613, 1746, 1812
Eco88I	CYCGRG	2 cut(s)	237, 500
EcoICRI	GAGCTC	1 cut(s)	1781
EcoRII	CCWGG	6 cut(s)	110, 357, 384, 1363, 1387, 1582
EcoT14I	CCWWGG	1 cut(s)	1125
EcoT22I	ATGCAT	1 cut(s)	1291
EcoT38I	GRGCYC	3 cut(s)	111, 351, 1783
EgeI	GGCGCC	2 cut(s)	462, 645
EheI	GGCGCC	2 cut(s)	462, 645
ErhI	CCWWGG	1 cut(s)	1125
FaeI	CATG	5 cut(s)	1198, 1289, 1373, 1401, 1765
FatI	CATG	5 cut(s)	1194, 1285, 1369, 1397, 1761
FauI	CCCGC	1 cut(s)	144
FbaI	TGATCA	1 cut(s)	703
FokI	GGATG	5 cut(s)	314, 392, 436, 1049, 1280
FriOI	GRGCYC	3 cut(s)	111, 351, 1783
FspBI	CTAG	5 cut(s)	786, 894, 915, 1497, 1694
GlaI	GCGC	7 cut(s)	150, 193, 397, 462, 507, 645, 987
GsaI	CCCAGC	1 cut(s)	290
GsuI	CTGGAG	3 cut(s)	245, 341, 407
HaeII	RGCGCY	5 cut(s)	195, 464, 509, 647, 989
HaeIII	GGCC	9 cut(s)	104, 109, 115, 164, 815, 831, 1360, 1595, 1661
HhaI	GCGC	7 cut(s)	151, 194, 398, 463, 508, 646, 988
Hin1I	GRCGYC	3 cut(s)	461, 528, 644
Hin1II	CATG	5 cut(s)	1198, 1289, 1373, 1401, 1765
Hin6I	GCGC	7 cut(s)	149, 192, 396, 461, 506, 644, 986
HinP1I	GCGC	7 cut(s)	149, 192, 396, 461, 506, 644, 986
HincII	GTYRAC	2 cut(s)	181, 1561
HindII	GTYRAC	2 cut(s)	181, 1561
HphI	GGTGA	4 cut(s)	737, 1249, 1415, 1800
Hpy166II	GTNNAC	8 cut(s)	181, 262, 321, 797, 1258, 1318, 1561, 1808
Hpy188I	TCNGA	9 cut(s)	36, 99, 126, 486, 565, 602, 711, 955, 1632
Hpy188III	TCNNGA	6 cut(s)	224, 410, 779, 821, 866, 1370
Hpy8I	GTNNAC	8 cut(s)	181, 262, 321, 797, 1258, 1318, 1561, 1808
Hpy99I	CGWCG	5 cut(s)	103, 329, 338, 530, 533
HpyAV	CCTTC	4 cut(s)	211, 223, 805, 1787
HpyCH4III	ACNGT	5 cut(s)	481, 624, 1057, 1255, 1474
HpyCH4IV	ACGT	3 cut(s)	76, 258, 528
HpyCH4V	TGCA	6 cut(s)	43, 716, 965, 1183, 1289, 1318
HpyF3I	CTNAG	7 cut(s)	53, 138, 443, 453, 579, 633, 1304
HpySE526I	ACGT	3 cut(s)	76, 258, 528
Hsp92I	GRCGYC	3 cut(s)	461, 528, 644
Hsp92II	CATG	5 cut(s)	1198, 1289, 1373, 1401, 1765
HspAI	GCGC	7 cut(s)	149, 192, 396, 461, 506, 644, 986
KasI	GGCGCC	2 cut(s)	460, 643
Kpn2I	TCCGGA	1 cut(s)	778
KroI	GCCGGC	1 cut(s)	553
KroNI	GCCGGC	1 cut(s)	555
Ksp22I	TGATCA	1 cut(s)	703
KspI	CCGCGG	1 cut(s)	50
LguI	GCTCTTC	2 cut(s)	723, 1632
LmnI	GCTCC	6 cut(s)	126, 283, 346, 360, 1272, 1378
Lsp1109I	GCAGC	5 cut(s)	495, 995, 1195, 1619, 1708
MabI	ACCWGGT	2 cut(s)	1387, 1582
MaeI	CTAG	5 cut(s)	786, 894, 915, 1497, 1694
MaeII	ACGT	3 cut(s)	76, 258, 528
MaeIII	GTNAC	4 cut(s)	1330, 1393, 1403, 1489
MbiI	CCGCTC	1 cut(s)	1535
MflI	RGATCY	3 cut(s)	232, 1121, 1499
MhlI	GDGCHC	6 cut(s)	111, 351, 1277, 1320, 1513, 1783
Mly113I	GGCGCC	2 cut(s)	461, 644
MlyI	GAGTC	5 cut(s)	31, 76, 77, 316, 1472
MmeI	TCCRAC	4 cut(s)	59, 122, 574, 1321
Mph1103I	ATGCAT	1 cut(s)	1291
MroI	TCCGGA	1 cut(s)	778
MroNI	GCCGGC	1 cut(s)	553
MseI	TTAA	8 cut(s)	201, 369, 546, 663, 1005, 1062, 1566, 1653
MspA1I	CMGCKG	3 cut(s)	49, 537, 1665
MspCI	CTTAAG	1 cut(s)	662
Mva1269I	GAATGC	1 cut(s)	1425
MvaI	CCWGG	6 cut(s)	112, 359, 386, 1365, 1389, 1584
MvnI	CGCG	4 cut(s)	49, 151, 338, 398
NaeI	GCCGGC	1 cut(s)	555
NarI	GGCGCC	2 cut(s)	461, 644
NciI	CCSGG	4 cut(s)	106, 117, 352, 1597
NgoMIV	GCCGGC	1 cut(s)	553
NlaIII	CATG	5 cut(s)	1198, 1289, 1373, 1401, 1765
NlaIV	GGNNCC	8 cut(s)	109, 234, 348, 356, 462, 645, 1123, 1715
NmeAIII	GCCGAG	3 cut(s)	190, 587, 1812
NmuCI	GTSAC	4 cut(s)	1330, 1393, 1403, 1489
NsiI	ATGCAT	1 cut(s)	1291
NspI	RCATGY	2 cut(s)	1289, 1401
PaeI	GCATGC	1 cut(s)	1289
PaeR7I	CTCGAG	1 cut(s)	500
PagI	TCATGA	1 cut(s)	1369
PceI	AGGCCT	2 cut(s)	815, 1360
PciI	ACATGT	1 cut(s)	1397
PciSI	GCTCTTC	2 cut(s)	723, 1632
PcsI	WCGNNNNNNNCGW	1 cut(s)	321
PctI	GAATGC	1 cut(s)	1425
PdiI	GCCGGC	1 cut(s)	555
PfeI	GAWTC	6 cut(s)	406, 597, 672, 918, 1199, 1240
PflFI	GACNNNGTC	1 cut(s)	530
Ple19I	CGATCG	3 cut(s)	124, 1439, 1711
PleI	GAGTC	5 cut(s)	31, 75, 77, 315, 1471
PluTI	GGCGCC	2 cut(s)	464, 647
PpsI	GAGTC	5 cut(s)	31, 75, 77, 315, 1471
Ppu21I	YACGTR	2 cut(s)	77, 259
PscI	ACATGT	1 cut(s)	1397
PshBI	ATTAAT	1 cut(s)	1566
Psp124BI	GAGCTC	1 cut(s)	1783
Psp6I	CCWGG	6 cut(s)	110, 357, 384, 1363, 1387, 1582
PspFI	CCCAGC	1 cut(s)	286
PspGI	CCWGG	6 cut(s)	110, 357, 384, 1363, 1387, 1582
PspN4I	GGNNCC	8 cut(s)	109, 234, 348, 356, 462, 645, 1123, 1715
PspOMI	GGGCCC	1 cut(s)	107
PspPI	GGNCC	4 cut(s)	107, 108, 114, 617
PspXI	VCTCGAGB	1 cut(s)	500
PstI	CTGCAG	1 cut(s)	967
PsuI	RGATCY	3 cut(s)	232, 1121, 1499
PsyI	GACNNNGTC	1 cut(s)	530
PvuI	CGATCG	3 cut(s)	124, 1439, 1711
PvuII	CAGCTG	1 cut(s)	1665
RsaI	GTAC	7 cut(s)	257, 272, 775, 807, 1259, 1347, 1650
RsaNI	GTAC	7 cut(s)	256, 271, 774, 806, 1258, 1346, 1649
SacI	GAGCTC	1 cut(s)	1783
SacII	CCGCGG	1 cut(s)	50
SapI	GCTCTTC	2 cut(s)	723, 1632
SaqAI	TTAA	8 cut(s)	201, 369, 546, 663, 1005, 1062, 1566, 1653
Sau96I	GGNCC	4 cut(s)	107, 108, 114, 617
ScaI	AGTACT	1 cut(s)	1347
SchI	GAGTC	5 cut(s)	31, 76, 77, 316, 1472
SduI	GDGCHC	6 cut(s)	111, 351, 1277, 1320, 1513, 1783
SexAI	ACCWGGT	2 cut(s)	1387, 1582
SfcI	CTRYAG	3 cut(s)	963, 1424, 1503
SfoI	GGCGCC	2 cut(s)	462, 645
Sfr274I	CTCGAG	1 cut(s)	500
Sfr303I	CCGCGG	1 cut(s)	50
SgrBI	CCGCGG	1 cut(s)	50
SinI	GGWCC	1 cut(s)	617
SlaI	CTCGAG	1 cut(s)	500
SmlI	CTYRAG	3 cut(s)	500, 662, 864
SmoI	CTYRAG	3 cut(s)	500, 662, 864
SnaBI	TACGTA	1 cut(s)	77
SphI	GCATGC	1 cut(s)	1289
SseBI	AGGCCT	2 cut(s)	815, 1360
SspDI	GGCGCC	2 cut(s)	460, 643
SspMI	CTAG	5 cut(s)	786, 894, 915, 1497, 1694
SstI	GAGCTC	1 cut(s)	1783
StuI	AGGCCT	2 cut(s)	815, 1360
StyI	CCWWGG	1 cut(s)	1125
TaaI	ACNGT	5 cut(s)	481, 624, 1057, 1255, 1474
TaiI	ACGT	3 cut(s)	79, 261, 531
TaqI	TCGA	3 cut(s)	501, 801, 1466
TaqII	GACCGA	1 cut(s)	1473
TatI	WGTACW	2 cut(s)	805, 1345
TauI	GCSGC	6 cut(s)	52, 65, 365, 553, 1079, 1769
TfiI	GAWTC	6 cut(s)	406, 597, 672, 918, 1199, 1240
Tru1I	TTAA	8 cut(s)	201, 369, 546, 663, 1005, 1062, 1566, 1653
Tru9I	TTAA	8 cut(s)	201, 369, 546, 663, 1005, 1062, 1566, 1653
TscAI	CASTG	3 cut(s)	895, 1060, 1260
TseFI	GTSAC	4 cut(s)	1330, 1393, 1403, 1489
TseI	GCWGC	5 cut(s)	508, 983, 1183, 1607, 1721
Tsp45I	GTSAC	4 cut(s)	1330, 1393, 1403, 1489
TspDTI	ATGAA	5 cut(s)	664, 864, 1053, 1327, 1713
TspGWI	ACGGA	5 cut(s)	76, 1275, 1292, 1636, 1828
TspRI	CASTG	3 cut(s)	895, 1060, 1260
Tth111I	GACNNNGTC	1 cut(s)	530
Vha464I	CTTAAG	1 cut(s)	662
VneI	GTGCAC	1 cut(s)	1316
VpaK11BI	GGWCC	1 cut(s)	617
VspI	ATTAAT	1 cut(s)	1566
XapI	RAATTY	3 cut(s)	835, 871, 1214
XceI	RCATGY	2 cut(s)	1289, 1401
XcmI	CCANNNNNNNNNTGG	1 cut(s)	930
XhoI	CTCGAG	1 cut(s)	500
XspI	CTAG	5 cut(s)	786, 894, 915, 1497, 1694
ZraI	GACGTC	1 cut(s)	529
ZrmI	AGTACT	1 cut(s)	1347
Zsp2I	ATGCAT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.