Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0003547

Physical Location & Seq

Genomic Coordinates Reverse (-)

57797577 .. 57803537

Gene Structure 5961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G038080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGCTTCAATTTCCGGCATTTGTTCCTCTTCTCCGACTCTGCAACCGCGGCTGAGACCGGCGGCGAGTCTATTACGTAAAGACTCCGTTTCGTTTCCGACGTCCCGGCCCAGGCCCGGCGATCGGAGCTTGTTCGCTCAGTCGGTGGCCCGGGAGATCAAGGCCGAGTTGGCCAAGGCCGACGGCGATGGCGCTCTGATTAAGGAGGAGAAGAAGAAGAAGGTTCTGGAGAAGGATCCTCGGGCGTTGTGGCGGAGGTACGTGGACTGGTTGTACCAGCACAAGGACCTAGGGCTGTATTTGGATGTGAGTCGGGTCGGGTTTACCGACGAGTTCGCCGCGGAAATGGAGCCCCGGCTCCAGGCGGCGTTTAAGGCCATGGAGGACCTGGAGAAAGGCGCGATTGCGAATCCCGATGAGGGGCGGATGGTCGGGCATTACTGGCTGAGAGACCCTAAGCGGGCGCCGAAACCGATGCTCACGGCTCAGATTGAGAACACGCTCGAGGCGCTGCTCAAGTTCTCCGACGACGTCGTCAGCGGTAAGATTAAGCCGCCGGCTTCTTCGGAGGGTCGTTTTACTCAGGTTCTTTCGGTTGGAATCGGAGGCTCGGCACTTGGACCACAGTTTGTTGCTGAGGCATTGGCGCCTGATGTTCCTCCTCTTAAGATACGATTCATTGACAATACCGATCCAGCAGGAATTGATCATCAGATTGCACAGCTCGGTGAAGAGCTGGCTTCTACTCTTGTAATTGTGATTTCAAAGAGTGGGGGTACTCCTGAAACTAGAAATGGTTTACTCGAAGTACAGAAGGCCTTTCGTGAGGCTGGCCTAAATTTCGCAAAACAGGGTGTGGCTATCACTCAAGAAAATTCATTATTAGACAACACTGCTAGAATTGAGGGCTGGTTAGCTAGATTCCCAATGTTTGACTGGGTTGGTGGAAGAACATCTGAAATGTCTGCAGTTGGTCTTCTTCCAGCAGCGCTTCAGGGAATTGACATTAAAGAAATGCTCGCTGGTGGAAAGTTGATGGATGAAGCTAATAGAACCACTGTGGTTAAGAATAATCCTGCTGCCTTACTTGCTTTATGCTGGTATTGGGCTACTGATGGATTGGGATCCAAGGATATGGTTGTTCTTCCTTACAAGGACAGCCTATTATTATTTAGTCGGTATTTGCAGCAGTTGGTCATGGAATCTATTGGGAAAGAATTTGACCTTGATGGTAATCGGGTGAATCAAGGACTTACAGTGTACGGAAACAAAGGGAGCACGGATCAGCATGCATACATCCAACAACTGAGAGATGGCGTGCACAATTTCTTTGTGACATTCATTGAAGTACTACGAGATAGGCCTCCTGGTCATGATTGGGAGCTTGAACCAGGTGTCACATGTGGTGACTATCTATTTGGAATGCTACAGGGAACACGATCGGCTCTTTATTCTAATGACCGAGAGTCGATTACGGTTTCTGTGGAAGAAGTGACACCTAGATCTGTAGGTGCTCTTATAGCACTTTATGAGAGAGCGGTTGGGATTTATGCCTCACTTGTCAACATTAATGCTTACCATCAACCTGGTGTGGAGGCCGGGAAGAAAGCAGCCGGAGAAGTATTAGCTCTTCAGAAACGGGTTTTATCCGTACTTAATGAGGCCAGCTGTAAAGAGCCTGTTGAGCCATTGACACTAGATGAAGTAGCCGATCGGTGCCACGCTGCTGAAGATATTGAAATGATTTACAAGATCATCGCTCACATGGCGGCAAATGACAGAGCTCTTATTGCTGAAGGCAGTTGCGGTTCACCACGGAGTGTCAAAGTTTTCCTCGGCGAGTGTAATGTGGAGGCATTATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009909 GO:0009911 GO:0009941 GO:0009987 GO:0016853 GO:0016860 GO:0016861 GO:0031967 GO:0031975 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PGI

Protein Analysis

621

Amino Acids

67.97

Weight (kDa)

5.48

Isoelectric Point (pI)

38.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGI	PF00342	126 - 452	3e-48	Phosphoglucose isomerase
PGI	PF00342	483 - 543	1.9e-09	Phosphoglucose isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24620 AT4G24620
fragaria_vesca	FvH4_5g35680 FvH4_5g35680
malus_domestica	MD08G1194100.v1.1 MD15G1382200.v1.1
prunus_persica	Prupe.1G528400_v2.0.a1 Prupe.1G528400_v2.0.a1
pyrus_communis	pycom15g34220
rosa_chinensis	RchiOBHm_Chr7g0237251 RchiOBHm_Chr7g0237271 RchiOBHm_Chr7g0237281 RchiOBHm_Chr7g0237311 RchiOBHm_Chr7g0237321
rosa_laevigata	RLG00000001012 RLG00000001018
rosa_multiflora	Rmu_sc0002923.1_g000043 Rmu_sc0002923.1_g000049 Rmu_sc0006438.1_g000001 Rmu_sc0006438.1_g000006 Rmu_sc0006438.1_g000007
rosa_roxburghii	Rroxscaffold_3G00224320
rosa_rugosa	Rorug07G0302600 Rorug07G0302700
rosa_samantha	Rh7AG459300 Rh7AG459500 Rh7AG459600 Rh7AG459700 Rh7AG459800 Rh7BG429700 Rh7CG477100 Rh7DG446400
rosa_wichuraiana	Rw0G003870 Rw7G038060 Rw7G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	533
AatII	GACGTC	2 cut(s)	104, 534
AccB1I	GGYRCC	3 cut(s)	463, 646, 1716
AccBSI	CCGCTC	1 cut(s)	1538
AccII	CGCG	3 cut(s)	49, 341, 401
AclWI	GGATC	6 cut(s)	230, 243, 686, 1119, 1132, 1290
AcoI	YGGCCR	1 cut(s)	171
AcsI	RAATTY	3 cut(s)	838, 874, 1217
AcuI	CTGAAG	4 cut(s)	977, 1616, 1749, 1815
AcyI	GRCGYC	4 cut(s)	101, 464, 531, 647
AdeI	CACNNNGTG	1 cut(s)	1820
AfaI	GTAC	7 cut(s)	260, 275, 778, 810, 1262, 1350, 1653
AfeI	AGCGCT	1 cut(s)	990
AfiI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 283, 361, 419, 420, 460
AflII	CTTAAG	1 cut(s)	665
AflIII	ACRYGT	1 cut(s)	1400
AgsI	TTSAA	5 cut(s)	9, 765, 1346, 1388, 1739
AhdI	GACNNNNNGTC	1 cut(s)	1465
AjnI	CCWGG	6 cut(s)	110, 360, 387, 1366, 1390, 1585
AluBI	AGCT	9 cut(s)	129, 724, 736, 917, 1046, 1384, 1628, 1668, 1784
AluI	AGCT	9 cut(s)	129, 724, 736, 917, 1046, 1384, 1628, 1668, 1784
Alw21I	GWGCWC	4 cut(s)	1280, 1323, 1516, 1786
Alw26I	GTCTC	2 cut(s)	49, 444
Alw44I	GTGCAC	1 cut(s)	1319
AlwI	GGATC	6 cut(s)	230, 243, 686, 1119, 1132, 1290
Ama87I	CYCGRG	3 cut(s)	150, 240, 503
Aor51HI	AGCGCT	1 cut(s)	990
ApaLI	GTGCAC	1 cut(s)	1319
ApeKI	GCWGC	6 cut(s)	511, 986, 1079, 1186, 1610, 1724
ApoI	RAATTY	3 cut(s)	838, 874, 1217
ArsI	GACNNNNNNTTYG	2 cut(s)	1400, 1432
AseI	ATTAAT	1 cut(s)	1569
AspA2I	CCTAGG	1 cut(s)	289
AspLEI	GCGC	6 cut(s)	194, 401, 466, 511, 649, 991
AspS9I	GGNCC	6 cut(s)	108, 114, 148, 286, 385, 620
AsuC2I	CCSGG	6 cut(s)	106, 117, 151, 152, 355, 1600
AsuHPI	GGTGA	4 cut(s)	740, 1252, 1418, 1803
AvaI	CYCGRG	3 cut(s)	150, 240, 503
AvaII	GGWCC	3 cut(s)	286, 385, 620
AvrII	CCTAGG	1 cut(s)	289
BaeGI	GKGCMC	1 cut(s)	1323
BalI	TGGCCA	1 cut(s)	173
BamHI	GGATCC	2 cut(s)	235, 1124
BanI	GGYRCC	3 cut(s)	463, 646, 1716
BanII	GRGCYC	2 cut(s)	354, 1786
BarI	GAAGNNNNNNTAC	2 cut(s)	1614, 1646
BbsI	GAAGAC	1 cut(s)	968
Bbv12I	GWGCWC	4 cut(s)	1280, 1323, 1516, 1786
BbvCI	CCTCAGC	1 cut(s)	636
BbvI	GCAGC	6 cut(s)	498, 998, 1066, 1198, 1622, 1711
BccI	CCATC	7 cut(s)	182, 421, 1030, 1109, 1223, 1307, 1587
BceAI	ACGGC	2 cut(s)	199, 498
BcgI	CGANNNNNNTGC	2 cut(s)	457, 491
BciT130I	CCWGG	6 cut(s)	112, 362, 389, 1368, 1392, 1587
BclI	TGATCA	1 cut(s)	706
BcnI	CCSGG	6 cut(s)	106, 117, 151, 152, 355, 1600
BcoDI	GTCTC	2 cut(s)	49, 444
BfaI	CTAG	6 cut(s)	290, 789, 897, 918, 1500, 1697
BfmI	CTRYAG	3 cut(s)	966, 1427, 1506
BfoI	RGCGCY	5 cut(s)	195, 467, 512, 650, 992
BfrI	CTTAAG	1 cut(s)	665
BglI	GCCNNNNNGGC	1 cut(s)	170
BglII	AGATCT	1 cut(s)	1502
BlnI	CCTAGG	1 cut(s)	289
BmcAI	AGTACT	1 cut(s)	1350
Bme18I	GGWCC	3 cut(s)	286, 385, 620
BmeRI	GACNNNNNGTC	1 cut(s)	1465
BmeT110I	CYCGRG	3 cut(s)	150, 240, 503
BmgT120I	GGNCC	6 cut(s)	108, 114, 148, 286, 385, 620
BmiI	GGNNCC	7 cut(s)	237, 351, 359, 465, 648, 1126, 1718
BmrI	ACTGGG	1 cut(s)	946
BmsI	GCATC	1 cut(s)	465
BmuI	ACTGGG	1 cut(s)	946
BpiI	GAAGAC	1 cut(s)	968
BpmI	CTGGAG	3 cut(s)	248, 344, 410
Bpu10I	CCTNAGC	2 cut(s)	456, 636
BpuEI	CTTGAG	2 cut(s)	500, 852
BpuMI	CCSGG	6 cut(s)	106, 117, 151, 152, 355, 1600
BsaAI	YACGTR	2 cut(s)	77, 262
BsaBI	GATNNNNATC	2 cut(s)	1123, 1233
BsaHI	GRCGYC	4 cut(s)	101, 464, 531, 647
BsaI	GGTCTC	2 cut(s)	49, 444
Bsc4I	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 283, 361, 419, 420, 460
Bse118I	RCCGGY	2 cut(s)	58, 556
Bse1I	ACTGG	3 cut(s)	272, 446, 941
Bse8I	GATNNNNATC	2 cut(s)	1123, 1233
BseBI	CCWGG	6 cut(s)	112, 362, 389, 1368, 1392, 1587
BseGI	GGATG	4 cut(s)	310, 432, 1045, 1296
BseJI	GATNNNNATC	2 cut(s)	1123, 1233
BseLI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 283, 361, 419, 420, 460
BseMII	CTCAG	7 cut(s)	44, 152, 437, 500, 596, 627, 1298
BseNI	ACTGG	3 cut(s)	272, 446, 941
BseRI	GAGGAG	2 cut(s)	221, 651
BseSI	GKGCMC	1 cut(s)	1323
BseXI	GCAGC	6 cut(s)	498, 998, 1066, 1198, 1622, 1711
Bsh1236I	CGCG	3 cut(s)	49, 341, 401
Bsh1285I	CGRYCG	3 cut(s)	124, 1442, 1714
BshNI	GGYRCC	3 cut(s)	463, 646, 1716
BsiEI	CGRYCG	3 cut(s)	124, 1442, 1714
BsiHKAI	GWGCWC	4 cut(s)	1280, 1323, 1516, 1786
BsiHKCI	CYCGRG	3 cut(s)	150, 240, 503
BsiSI	CCGG	9 cut(s)	15, 59, 106, 117, 151, 355, 557, 1599, 1614
BslFI	GGGAC	1 cut(s)	88
BslI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 283, 361, 419, 420, 460
BsmAI	GTCTC	2 cut(s)	49, 444
BsmFI	GGGAC	1 cut(s)	88
BsmI	GAATGC	1 cut(s)	1428
Bso31I	GGTCTC	2 cut(s)	49, 444
BsoBI	CYCGRG	3 cut(s)	150, 240, 503
Bsp1286I	GDGCHC	5 cut(s)	354, 1280, 1323, 1516, 1786
Bsp19I	CCATGG	1 cut(s)	378
BspCNI	CTCAG	7 cut(s)	45, 151, 438, 499, 595, 628, 1299
BspFNI	CGCG	3 cut(s)	49, 341, 401
BspHI	TCATGA	1 cut(s)	1372
BspLI	GGNNCC	7 cut(s)	237, 351, 359, 465, 648, 1126, 1718
BspMAI	CTGCAG	1 cut(s)	970
BspPI	GGATC	6 cut(s)	230, 243, 686, 1119, 1132, 1290
BspQI	GCTCTTC	2 cut(s)	726, 1635
BspT107I	GGYRCC	3 cut(s)	463, 646, 1716
BspTI	CTTAAG	1 cut(s)	665
BspTNI	GGTCTC	2 cut(s)	49, 444
BsrBI	CCGCTC	1 cut(s)	1538
BsrFI	RCCGGY	2 cut(s)	58, 556
BsrI	ACTGG	3 cut(s)	272, 446, 941
BssAI	RCCGGY	2 cut(s)	58, 556
BssNI	GRCGYC	4 cut(s)	101, 464, 531, 647
BssT1I	CCWWGG	4 cut(s)	174, 289, 378, 1128
Bst2UI	CCWGG	6 cut(s)	112, 362, 389, 1368, 1392, 1587
Bst4CI	ACNGT	4 cut(s)	627, 1060, 1258, 1477
Bst6I	CTCTTC	3 cut(s)	34, 726, 1635
BstACI	GRCGYC	4 cut(s)	101, 464, 531, 647
BstAFI	CTTAAG	1 cut(s)	665
BstBAI	YACGTR	2 cut(s)	77, 262
BstC8I	GCNNGC	8 cut(s)	462, 558, 738, 832, 1020, 1290, 1319, 1666
BstDEI	CTNAG	8 cut(s)	53, 138, 446, 456, 486, 582, 636, 1307
BstDSI	CCRYGG	4 cut(s)	47, 339, 378, 1814
BstF5I	GGATG	4 cut(s)	310, 432, 1045, 1296
BstFNI	CGCG	3 cut(s)	49, 341, 401
BstH2I	RGCGCY	5 cut(s)	195, 467, 512, 650, 992
BstHHI	GCGC	6 cut(s)	194, 401, 466, 511, 649, 991
BstMAI	GTCTC	2 cut(s)	49, 444
BstMCI	CGRYCG	3 cut(s)	124, 1442, 1714
BstNI	CCWGG	6 cut(s)	112, 362, 389, 1368, 1392, 1587
BstNSI	RCATGY	2 cut(s)	1292, 1404
BstSFI	CTRYAG	3 cut(s)	966, 1427, 1506
BstSLI	GKGCMC	1 cut(s)	1323
BstSNI	TACGTA	1 cut(s)	77
BstUI	CGCG	3 cut(s)	49, 341, 401
BstV1I	GCAGC	6 cut(s)	498, 998, 1066, 1198, 1622, 1711
BstV2I	GAAGAC	1 cut(s)	968
BstX2I	RGATCY	3 cut(s)	235, 1124, 1502
BstXI	CCANNNNNNTGG	2 cut(s)	1135, 1586
BstYI	RGATCY	3 cut(s)	235, 1124, 1502
BtgI	CCRYGG	4 cut(s)	47, 339, 378, 1814
BtgZI	GCGATG	2 cut(s)	201, 1741
BtsCI	GGATG	4 cut(s)	310, 432, 1045, 1296
BtsI	GCAGTG	1 cut(s)	891
BtsIMutI	CAGTG	3 cut(s)	891, 1056, 1263
Cac8I	GCNNGC	8 cut(s)	462, 558, 738, 832, 1020, 1290, 1319, 1666
CciI	TCATGA	1 cut(s)	1372
CfoI	GCGC	6 cut(s)	194, 401, 466, 511, 649, 991
Cfr10I	RCCGGY	2 cut(s)	58, 556
Cfr13I	GGNCC	6 cut(s)	108, 114, 148, 286, 385, 620
Cfr42I	CCGCGG	2 cut(s)	50, 342
Cfr9I	CCCGGG	1 cut(s)	150
CsiI	ACCWGGT	2 cut(s)	1390, 1585
Csp6I	GTAC	7 cut(s)	259, 274, 777, 809, 1261, 1349, 1652
CviAII	CATG	6 cut(s)	379, 1198, 1289, 1373, 1401, 1765
CviQI	GTAC	7 cut(s)	259, 274, 777, 809, 1261, 1349, 1652
DdeI	CTNAG	8 cut(s)	53, 138, 446, 456, 486, 582, 636, 1307
DinI	GGCGCC	2 cut(s)	465, 648
DraIII	CACNNNGTG	1 cut(s)	1820
DrdI	GACNNNNNNGTC	1 cut(s)	533
DriI	GACNNNNNGTC	1 cut(s)	1465
DseDI	GACNNNNNNGTC	1 cut(s)	533
EaeI	YGGCCR	1 cut(s)	171
Eam1104I	CTCTTC	3 cut(s)	34, 726, 1635
Eam1105I	GACNNNNNGTC	1 cut(s)	1465
EarI	CTCTTC	3 cut(s)	34, 726, 1635
EciI	GGCGGA	2 cut(s)	268, 439
Ecl136II	GAGCTC	1 cut(s)	1784
Eco105I	TACGTA	1 cut(s)	77
Eco130I	CCWWGG	4 cut(s)	174, 289, 378, 1128
Eco147I	AGGCCT	2 cut(s)	818, 1363
Eco24I	GRGCYC	2 cut(s)	354, 1786
Eco31I	GGTCTC	2 cut(s)	49, 444
Eco47I	GGWCC	3 cut(s)	286, 385, 620
Eco47III	AGCGCT	1 cut(s)	990
Eco53kI	GAGCTC	1 cut(s)	1784
Eco57I	CTGAAG	4 cut(s)	977, 1616, 1749, 1815
Eco88I	CYCGRG	3 cut(s)	150, 240, 503
EcoICRI	GAGCTC	1 cut(s)	1784
EcoO109I	RGGNCCY	2 cut(s)	286, 385
EcoRII	CCWGG	6 cut(s)	110, 360, 387, 1366, 1390, 1585
EcoT14I	CCWWGG	4 cut(s)	174, 289, 378, 1128
EcoT22I	ATGCAT	1 cut(s)	1294
EcoT38I	GRGCYC	2 cut(s)	354, 1786
EgeI	GGCGCC	2 cut(s)	465, 648
EheI	GGCGCC	2 cut(s)	465, 648
ErhI	CCWWGG	4 cut(s)	174, 289, 378, 1128
FaeI	CATG	6 cut(s)	382, 1201, 1292, 1376, 1404, 1768
FaqI	GGGAC	1 cut(s)	88
FatI	CATG	6 cut(s)	378, 1197, 1288, 1372, 1400, 1764
FauI	CCCGC	1 cut(s)	453
FbaI	TGATCA	1 cut(s)	706
FokI	GGATG	4 cut(s)	317, 439, 1052, 1283
FriOI	GRGCYC	2 cut(s)	354, 1786
FspBI	CTAG	6 cut(s)	290, 789, 897, 918, 1500, 1697
GlaI	GCGC	6 cut(s)	193, 400, 465, 510, 648, 990
GsuI	CTGGAG	3 cut(s)	248, 344, 410
HaeII	RGCGCY	5 cut(s)	195, 467, 512, 650, 992
HapII	CCGG	9 cut(s)	15, 59, 106, 117, 151, 355, 557, 1599, 1614
HhaI	GCGC	6 cut(s)	194, 401, 466, 511, 649, 991
Hin1I	GRCGYC	4 cut(s)	101, 464, 531, 647
Hin1II	CATG	6 cut(s)	382, 1201, 1292, 1376, 1404, 1768
Hin6I	GCGC	6 cut(s)	192, 399, 464, 509, 647, 989
HinP1I	GCGC	6 cut(s)	192, 399, 464, 509, 647, 989
HincII	GTYRAC	1 cut(s)	1564
HindII	GTYRAC	1 cut(s)	1564
HpaII	CCGG	9 cut(s)	15, 59, 106, 117, 151, 355, 557, 1599, 1614
HphI	GGTGA	4 cut(s)	740, 1252, 1418, 1803
Hpy166II	GTNNAC	7 cut(s)	265, 324, 800, 1261, 1321, 1564, 1811
Hpy188III	TCNNGA	6 cut(s)	227, 413, 782, 824, 869, 1373
Hpy8I	GTNNAC	7 cut(s)	265, 324, 800, 1261, 1321, 1564, 1811
Hpy99I	CGWCG	6 cut(s)	103, 185, 332, 530, 533, 536
HpyAV	CCTTC	4 cut(s)	214, 226, 808, 1790
HpyCH4III	ACNGT	4 cut(s)	627, 1060, 1258, 1477
HpyCH4IV	ACGT	4 cut(s)	76, 101, 261, 531
HpyCH4V	TGCA	6 cut(s)	43, 719, 968, 1186, 1292, 1321
HpyF3I	CTNAG	8 cut(s)	53, 138, 446, 456, 486, 582, 636, 1307
HpySE526I	ACGT	4 cut(s)	76, 101, 261, 531
Hsp92I	GRCGYC	4 cut(s)	101, 464, 531, 647
Hsp92II	CATG	6 cut(s)	382, 1201, 1292, 1376, 1404, 1768
HspAI	GCGC	6 cut(s)	192, 399, 464, 509, 647, 989
KasI	GGCGCC	2 cut(s)	463, 646
KroI	GCCGGC	1 cut(s)	556
KroNI	GCCGGC	1 cut(s)	558
Ksp22I	TGATCA	1 cut(s)	706
KspI	CCGCGG	2 cut(s)	50, 342
LguI	GCTCTTC	2 cut(s)	726, 1635
LmnI	GCTCC	5 cut(s)	126, 349, 363, 1275, 1381
Lsp1109I	GCAGC	6 cut(s)	498, 998, 1066, 1198, 1622, 1711
LweI	GCATC	1 cut(s)	465
MabI	ACCWGGT	2 cut(s)	1390, 1585
MaeI	CTAG	6 cut(s)	290, 789, 897, 918, 1500, 1697
MaeII	ACGT	4 cut(s)	76, 101, 261, 531
MaeIII	GTNAC	4 cut(s)	1333, 1396, 1406, 1492
MbiI	CCGCTC	1 cut(s)	1538
MflI	RGATCY	3 cut(s)	235, 1124, 1502
MhlI	GDGCHC	5 cut(s)	354, 1280, 1323, 1516, 1786
MlsI	TGGCCA	1 cut(s)	173
MluCI	AATT	9 cut(s)	9, 702, 753, 838, 874, 900, 999, 1217, 1324
MluNI	TGGCCA	1 cut(s)	173
Mly113I	GGCGCC	2 cut(s)	464, 647
MlyI	GAGTC	5 cut(s)	31, 76, 77, 319, 1475
MmeI	TCCRAC	5 cut(s)	59, 122, 549, 577, 1324
Mox20I	TGGCCA	1 cut(s)	173
Mph1103I	ATGCAT	1 cut(s)	1294
MroNI	GCCGGC	1 cut(s)	556
MscI	TGGCCA	1 cut(s)	173
MseI	TTAA	8 cut(s)	201, 372, 549, 666, 1008, 1065, 1569, 1656
Msp20I	TGGCCA	1 cut(s)	173
MspA1I	CMGCKG	4 cut(s)	49, 341, 540, 1668
MspCI	CTTAAG	1 cut(s)	665
MspI	CCGG	9 cut(s)	15, 59, 106, 117, 151, 355, 557, 1599, 1614
Mva1269I	GAATGC	1 cut(s)	1428
MvaI	CCWGG	6 cut(s)	112, 362, 389, 1368, 1392, 1587
MvnI	CGCG	3 cut(s)	49, 341, 401
NaeI	GCCGGC	1 cut(s)	558
NarI	GGCGCC	2 cut(s)	464, 647
NciI	CCSGG	6 cut(s)	106, 117, 151, 152, 355, 1600
NcoI	CCATGG	1 cut(s)	378
NgoMIV	GCCGGC	1 cut(s)	556
NlaIII	CATG	6 cut(s)	382, 1201, 1292, 1376, 1404, 1768
NlaIV	GGNNCC	7 cut(s)	237, 351, 359, 465, 648, 1126, 1718
NmeAIII	GCCGAG	3 cut(s)	190, 590, 1815
NmuCI	GTSAC	4 cut(s)	1333, 1396, 1406, 1492
NsiI	ATGCAT	1 cut(s)	1294
NspI	RCATGY	2 cut(s)	1292, 1404
PaeI	GCATGC	1 cut(s)	1292
PaeR7I	CTCGAG	1 cut(s)	503
PagI	TCATGA	1 cut(s)	1372
PceI	AGGCCT	2 cut(s)	818, 1363
PciI	ACATGT	1 cut(s)	1400
PciSI	GCTCTTC	2 cut(s)	726, 1635
PcsI	WCGNNNNNNNCGW	2 cut(s)	98, 324
PctI	GAATGC	1 cut(s)	1428
PdiI	GCCGGC	1 cut(s)	558
PfeI	GAWTC	6 cut(s)	409, 600, 675, 921, 1202, 1243
PflFI	GACNNNGTC	2 cut(s)	530, 533
Ple19I	CGATCG	3 cut(s)	124, 1442, 1714
PleI	GAGTC	5 cut(s)	31, 75, 77, 318, 1474
PluTI	GGCGCC	2 cut(s)	467, 650
PpsI	GAGTC	5 cut(s)	31, 75, 77, 318, 1474
Ppu21I	YACGTR	2 cut(s)	77, 262
PpuMI	RGGWCCY	2 cut(s)	286, 385
PscI	ACATGT	1 cut(s)	1400
PshBI	ATTAAT	1 cut(s)	1569
Psp124BI	GAGCTC	1 cut(s)	1786
Psp5II	RGGWCCY	2 cut(s)	286, 385
Psp6I	CCWGG	6 cut(s)	110, 360, 387, 1366, 1390, 1585
PspGI	CCWGG	6 cut(s)	110, 360, 387, 1366, 1390, 1585
PspN4I	GGNNCC	7 cut(s)	237, 351, 359, 465, 648, 1126, 1718
PspPI	GGNCC	6 cut(s)	108, 114, 148, 286, 385, 620
PspPPI	RGGWCCY	2 cut(s)	286, 385
PspXI	VCTCGAGB	1 cut(s)	503
PstI	CTGCAG	1 cut(s)	970
PsuI	RGATCY	3 cut(s)	235, 1124, 1502
PsyI	GACNNNGTC	2 cut(s)	530, 533
PvuI	CGATCG	3 cut(s)	124, 1442, 1714
PvuII	CAGCTG	1 cut(s)	1668
RsaI	GTAC	7 cut(s)	260, 275, 778, 810, 1262, 1350, 1653
RsaNI	GTAC	7 cut(s)	259, 274, 777, 809, 1261, 1349, 1652
SacI	GAGCTC	1 cut(s)	1786
SacII	CCGCGG	2 cut(s)	50, 342
SapI	GCTCTTC	2 cut(s)	726, 1635
SaqAI	TTAA	8 cut(s)	201, 372, 549, 666, 1008, 1065, 1569, 1656
Sau96I	GGNCC	6 cut(s)	108, 114, 148, 286, 385, 620
ScaI	AGTACT	1 cut(s)	1350
SchI	GAGTC	5 cut(s)	31, 76, 77, 319, 1475
SduI	GDGCHC	5 cut(s)	354, 1280, 1323, 1516, 1786
SexAI	ACCWGGT	2 cut(s)	1390, 1585
SfaNI	GCATC	1 cut(s)	465
SfcI	CTRYAG	3 cut(s)	966, 1427, 1506
SfiI	GGCCNNNNNGGCC	1 cut(s)	170
SfoI	GGCGCC	2 cut(s)	465, 648
Sfr274I	CTCGAG	1 cut(s)	503
Sfr303I	CCGCGG	2 cut(s)	50, 342
SgrBI	CCGCGG	2 cut(s)	50, 342
SinI	GGWCC	3 cut(s)	286, 385, 620
SlaI	CTCGAG	1 cut(s)	503
SmaI	CCCGGG	1 cut(s)	152
SmlI	CTYRAG	4 cut(s)	503, 515, 665, 867
SmoI	CTYRAG	4 cut(s)	503, 515, 665, 867
SnaBI	TACGTA	1 cut(s)	77
SphI	GCATGC	1 cut(s)	1292
Sse9I	AATT	9 cut(s)	9, 702, 753, 838, 874, 900, 999, 1217, 1324
SseBI	AGGCCT	2 cut(s)	818, 1363
SspDI	GGCGCC	2 cut(s)	463, 646
SspMI	CTAG	6 cut(s)	290, 789, 897, 918, 1500, 1697
SstI	GAGCTC	1 cut(s)	1786
StuI	AGGCCT	2 cut(s)	818, 1363
StyI	CCWWGG	4 cut(s)	174, 289, 378, 1128
TaaI	ACNGT	4 cut(s)	627, 1060, 1258, 1477
TaiI	ACGT	4 cut(s)	79, 104, 264, 534
TaqI	TCGA	3 cut(s)	504, 804, 1469
TaqII	GACCGA	1 cut(s)	1476
TasI	AATT	9 cut(s)	9, 702, 753, 838, 874, 900, 999, 1217, 1324
TatI	WGTACW	2 cut(s)	808, 1348
TauI	GCSGC	6 cut(s)	52, 65, 341, 368, 556, 1772
TfiI	GAWTC	6 cut(s)	409, 600, 675, 921, 1202, 1243
Tru1I	TTAA	8 cut(s)	201, 372, 549, 666, 1008, 1065, 1569, 1656
Tru9I	TTAA	8 cut(s)	201, 372, 549, 666, 1008, 1065, 1569, 1656
TscAI	CASTG	3 cut(s)	898, 1063, 1263
TseFI	GTSAC	4 cut(s)	1333, 1396, 1406, 1492
TseI	GCWGC	6 cut(s)	511, 986, 1079, 1186, 1610, 1724
Tsp45I	GTSAC	4 cut(s)	1333, 1396, 1406, 1492
TspDTI	ATGAA	5 cut(s)	667, 867, 1056, 1330, 1716
TspGWI	ACGGA	5 cut(s)	76, 1278, 1295, 1639, 1831
TspMI	CCCGGG	1 cut(s)	150
TspRI	CASTG	3 cut(s)	898, 1063, 1263
Tth111I	GACNNNGTC	2 cut(s)	530, 533
Vha464I	CTTAAG	1 cut(s)	665
VneI	GTGCAC	1 cut(s)	1319
VpaK11BI	GGWCC	3 cut(s)	286, 385, 620
VspI	ATTAAT	1 cut(s)	1569
XapI	RAATTY	3 cut(s)	838, 874, 1217
XceI	RCATGY	2 cut(s)	1292, 1404
XcmI	CCANNNNNNNNNTGG	2 cut(s)	385, 933
XhoI	CTCGAG	1 cut(s)	503
XmaI	CCCGGG	1 cut(s)	150
XmaJI	CCTAGG	1 cut(s)	289
XspI	CTAG	6 cut(s)	290, 789, 897, 918, 1500, 1697
ZraI	GACGTC	2 cut(s)	102, 532
ZrmI	AGTACT	1 cut(s)	1350
Zsp2I	ATGCAT	1 cut(s)	1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.