Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003547

Physical Location & Seq

Genomic Coordinates Forward (+)

47118820 .. 47125706

Gene Structure 6887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1382200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGCTTCAATCTCCGGCATTTACTCTGCTTCTCCGACGATCACGCGCCACCTGAAACCGGCGAGTTCGCTGCGGAAGGATTCCGCTTCGGTTTCTGTTGCGTTTCCCGGCCGGATCAAATCGGCCGATCGCGGCTTTGCTCTGTCGGTGGCTCGGGACGTCTCGGCGGAGTTAGCGAAGACCACCGATGGGGCTCCGAGTAAGAAGAAGGGGCTGGTGAAGGACCCACACGCTCTGTGGCGGAGGTACGTTGACTGGCTGTACCAGCACAAGGAGCTCGGGTTGTTTCTGGATGTGAGTCGGGTCGGGTTCACGGACGAGTTCGTTTCCGAAATGGAGCCCCGGTTCCAGGCGGCGTTCAAGGCCATGGAGGAGCTGGAGAAGGGCGCGATTGCTAATCCCGATGAGCGCCGCATGGTCGGCCATTACTGGCTCCGAAACCCAAAGCTCGCCCCCACCTCGTTCCTCAGGTTGCAGATTGAGAAGACCCTCGAGGCACTTCTGAAGTTCTCGAACGACGTCGTCAGCGGTAAGATTAAGCCGCCATCTTCGCCGGAAGGTCGGTTTACTCAGATACTATCAGTTGGAATTGGAGGCTCAGCACTTGGACCACAGTTCGTTGCTGAGGCATTAGCTCCGGATAACCCTCCTCTTAAGATAAGATTTATTGACAATACCGATCCAGGAGGAATTGATCATCAGATTGCACAGCTTGGGCCCGAGCTGGCTTCAACACTTGTCATTGTGATTTCAAAGAGTGGAGGTACTCCTGAAACTAGAAATGGGTTACTGGAAGTACAGAAGGCCTTCCGTGAGGCTGGCCTGAACTTTGCAAAACAGGGTGTCGCTATAACTCAAGAAAATTCATTATTGGACAACACTGCCAGAATTGAGGGATGGTTAGCTAGATTCCCCATGTTTGACTGGGTTGGTGGCAGAACATCTGAAATGTCTGCAGTTGGTCTTCTTCCGGCAGCACTGCAGGGGATTGATATTAAAGAAATGCTCGCTGGTGGAGACTTGATGGATGAGGCAAACCGAACCACTGTGCTTAGGAATAATCCGGCTGCGTTGCTTGCTTTATGCTGGTATTGGGCTTCTGATGGGGTGGGATCAAAGGATATGGTTGTTCTTCCTTACAAGGATAGCCTATTATTATTTAGTCGGTATTTACAACAGCTGGTCATGGAATCTATTGGGAAGGAATTTGACCTTGATGGAAATCGGGTGAATCAAGGACTTACTGTCTATGGAAATAAAGGGAGCACAGATCAGCATGCCTACATTCAACAACTGAGAGACGGCGTGCACAATTTCTTTGCGACATTCATTGAAGTACTACGTGATAGGCCGCCAGGTCATGATTGGGAGCTTGAACCCGGCGTCACATGTGGTGACTACCTGTTCGGAATGCTGCAGGGAACAAGGTCAGCTCTTTATTCAAACGACCGAGAGTCGATTACGGTTACAGTGGAAGAAGTGACACCAAGATCTGTTGGCGCTCTTATAGCACTTTATGAGAGAGCAGTCGGGATATATGCTTCACTTGTCAACATTAACGCTTACCATCAACCTGGTGTTGAAGCTGGGAAAAAGGCAGCTGGAGAAGTATTAGCCCTTCAGAAGCGGGTTTTAGCAGTGCTCAATGAGGCCAGCTGCAAGGATCCTGTCGAACCATTGACGCTTGAAGAAGTGGCTGACCGGTGCCACGCTACTGAAGATATTGAAATGATCTACAAGATCATCGCTCACATGGCGGCCAATGACAGAGCAATTATCGCTGAAGGCAACTGCGGTTCACCGCGAAGCATCAAAGTTTTCGGCGGGGAGTGTAATGTCGATGCGTTGTACGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009909 GO:0009911 GO:0009941 GO:0009987 GO:0016853 GO:0016860 GO:0016861 GO:0031967 GO:0031975 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PGI

Protein Analysis

619

Amino Acids

67.45

Weight (kDa)

5.71

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGI	PF00342	122 - 448	1.2e-46	Phosphoglucose isomerase
PGI	PF00342	479 - 539	1.2e-09	Phosphoglucose isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24620 AT4G24620
fragaria_vesca	FvH4_5g35680 FvH4_5g35680
malus_domestica	MD08G1194100.v1.1 MD15G1382200.v1.1
prunus_persica	Prupe.1G528400_v2.0.a1 Prupe.1G528400_v2.0.a1
pyrus_communis	pycom15g34220
rosa_chinensis	RchiOBHm_Chr7g0237251 RchiOBHm_Chr7g0237271 RchiOBHm_Chr7g0237281 RchiOBHm_Chr7g0237311 RchiOBHm_Chr7g0237321
rosa_laevigata	RLG00000001012 RLG00000001018
rosa_multiflora	Rmu_sc0002923.1_g000043 Rmu_sc0002923.1_g000049 Rmu_sc0006438.1_g000001 Rmu_sc0006438.1_g000006 Rmu_sc0006438.1_g000007
rosa_roxburghii	Rroxscaffold_3G00224320
rosa_rugosa	Rorug07G0302600 Rorug07G0302700
rosa_samantha	Rh7AG459300 Rh7AG459500 Rh7AG459600 Rh7AG459700 Rh7AG459800 Rh7BG429700 Rh7CG477100 Rh7DG446400
rosa_wichuraiana	Rw0G003870 Rw7G038060 Rw7G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	162, 522
AbsI	CCTCGAGG	1 cut(s)	491
AccB1I	GGYRCC	1 cut(s)	1704
AccII	CGCG	4 cut(s)	46, 132, 389, 1804
AccIII	TCCGGA	1 cut(s)	637
AclWI	GGATC	5 cut(s)	122, 674, 1120, 1658, 1671
AcoI	YGGCCR	4 cut(s)	109, 123, 421, 1758
AcsI	RAATTY	2 cut(s)	862, 1205
AcuI	CTGAAG	4 cut(s)	524, 1604, 1737, 1803
AcyI	GRCGYC	3 cut(s)	159, 519, 1383
AfaI	GTAC	6 cut(s)	248, 263, 766, 798, 1338, 1850
AfiI	CCNNNNNNNGG	4 cut(s)	58, 189, 271, 349
AflII	CTTAAG	1 cut(s)	653
AflIII	ACRYGT	1 cut(s)	1388
AgeI	ACCGGT	1 cut(s)	1701
AhdI	GACNNNNNGTC	2 cut(s)	1244, 1453
AjnI	CCWGG	4 cut(s)	348, 682, 1354, 1573
AloI	GAACNNNNNNTCC	2 cut(s)	329, 361
Alw21I	GWGCWC	4 cut(s)	279, 1268, 1311, 1644
Alw26I	GTCTC	3 cut(s)	166, 1011, 1293
Alw44I	GTGCAC	1 cut(s)	1307
AlwI	GGATC	5 cut(s)	122, 674, 1120, 1658, 1671
Ama87I	CYCGRG	4 cut(s)	153, 278, 491, 719
Aor13HI	TCCGGA	1 cut(s)	637
ApaI	GGGCCC	1 cut(s)	720
ApaLI	GTGCAC	1 cut(s)	1307
ApeKI	GCWGC	6 cut(s)	70, 974, 1067, 1414, 1598, 1656
ApoI	RAATTY	2 cut(s)	862, 1205
ArsI	GACNNNNNNTTYG	2 cut(s)	1388, 1420
AsiGI	ACCGGT	1 cut(s)	1701
Asp700I	GAANNNNTTC	1 cut(s)	806
AspLEI	GCGC	4 cut(s)	48, 389, 411, 1502
AspS9I	GGNCC	4 cut(s)	223, 608, 716, 717
AsuC2I	CCSGG	3 cut(s)	108, 343, 1380
AsuHPI	GGTGA	4 cut(s)	229, 1240, 1406, 1791
AvaI	CYCGRG	4 cut(s)	153, 278, 491, 719
AvaII	GGWCC	2 cut(s)	223, 608
AxyI	CCTNAGG	1 cut(s)	467
BaeGI	GKGCMC	2 cut(s)	720, 1311
BamHI	GGATCC	1 cut(s)	1663
BanI	GGYRCC	1 cut(s)	1704
BanII	GRGCYC	4 cut(s)	196, 279, 342, 720
BarI	GAAGNNNNNNTAC	2 cut(s)	1602, 1634
BbsI	GAAGAC	3 cut(s)	185, 491, 956
Bbv12I	GWGCWC	4 cut(s)	279, 1268, 1311, 1644
BbvCI	CCTCAGC	1 cut(s)	624
BbvI	GCAGC	6 cut(s)	57, 986, 1054, 1401, 1610, 1643
BccI	CCATC	7 cut(s)	182, 553, 891, 1018, 1097, 1211, 1575
BceAI	ACGGC	1 cut(s)	1318
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	4 cut(s)	350, 684, 1356, 1575
BclI	TGATCA	1 cut(s)	694
BcnI	CCSGG	3 cut(s)	108, 343, 1380
BcoDI	GTCTC	3 cut(s)	166, 1011, 1293
BfaI	CTAG	2 cut(s)	777, 906
BfmI	CTRYAG	3 cut(s)	954, 980, 1415
BfoI	RGCGCY	2 cut(s)	412, 1503
BfrI	CTTAAG	1 cut(s)	653
BglII	AGATCT	1 cut(s)	1490
BlpI	GCTNAGC	1 cut(s)	598
BmcAI	AGTACT	1 cut(s)	1338
Bme1390I	CCNGG	7 cut(s)	108, 343, 350, 684, 1356, 1380, 1575
Bme18I	GGWCC	2 cut(s)	223, 608
BmeRI	GACNNNNNGTC	2 cut(s)	1244, 1453
BmeT110I	CYCGRG	4 cut(s)	153, 278, 491, 719
BmgT120I	GGNCC	4 cut(s)	223, 608, 716, 717
BmiI	GGNNCC	8 cut(s)	195, 225, 339, 347, 434, 718, 1665, 1706
BmrFI	CCNGG	7 cut(s)	108, 343, 350, 684, 1356, 1380, 1575
BmrI	ACTGGG	1 cut(s)	934
BmsI	GCATC	2 cut(s)	1818, 1831
BmuI	ACTGGG	1 cut(s)	934
BpiI	GAAGAC	3 cut(s)	185, 491, 956
BpmI	CTGGAG	2 cut(s)	398, 1623
Bpu10I	CCTNAGC	2 cut(s)	624, 1052
Bpu1102I	GCTNAGC	1 cut(s)	598
BpuEI	CTTGAG	1 cut(s)	840
BpuMI	CCSGG	3 cut(s)	108, 343, 1380
BsaAI	YACGTR	1 cut(s)	1343
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaHI	GRCGYC	3 cut(s)	159, 519, 1383
BsaJI	CCNNGG	2 cut(s)	341, 366
BsaWI	WCCGGW	2 cut(s)	637, 1701
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 329, 359
Bsc4I	CCNNNNNNNGG	4 cut(s)	58, 189, 271, 349
Bse118I	RCCGGY	2 cut(s)	58, 1701
Bse1I	ACTGG	4 cut(s)	260, 434, 795, 929
Bse21I	CCTNAGG	1 cut(s)	467
Bse8I	GATNNNNATC	1 cut(s)	1221
BseAI	TCCGGA	1 cut(s)	637
BseBI	CCWGG	4 cut(s)	350, 684, 1356, 1575
BseDI	CCNNGG	2 cut(s)	341, 366
BseGI	GGATG	3 cut(s)	298, 902, 1033
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	4 cut(s)	58, 189, 271, 349
BseMII	CTCAG	5 cut(s)	481, 584, 612, 615, 1286
BseNI	ACTGG	4 cut(s)	260, 434, 795, 929
BseRI	GAGGAG	2 cut(s)	386, 639
BseSI	GKGCMC	2 cut(s)	720, 1311
BseX3I	CGGCCG	2 cut(s)	109, 123
BseXI	GCAGC	6 cut(s)	57, 986, 1054, 1401, 1610, 1643
BseYI	CCCAGC	1 cut(s)	1586
Bsh1236I	CGCG	4 cut(s)	46, 132, 389, 1804
Bsh1285I	CGRYCG	4 cut(s)	112, 126, 130, 1450
BshNI	GGYRCC	1 cut(s)	1704
BshTI	ACCGGT	1 cut(s)	1701
BsiEI	CGRYCG	4 cut(s)	112, 126, 130, 1450
BsiHKAI	GWGCWC	4 cut(s)	279, 1268, 1311, 1644
BsiHKCI	CYCGRG	4 cut(s)	153, 278, 491, 719
BslFI	GGGAC	1 cut(s)	170
BslI	CCNNNNNNNGG	4 cut(s)	58, 189, 271, 349
BsmAI	GTCTC	3 cut(s)	166, 1011, 1293
BsmBI	CGTCTC	2 cut(s)	166, 1293
BsmFI	GGGAC	1 cut(s)	170
BsmI	GAATGC	1 cut(s)	1416
BsoBI	CYCGRG	4 cut(s)	153, 278, 491, 719
Bsp120I	GGGCCC	1 cut(s)	716
Bsp1286I	GDGCHC	7 cut(s)	196, 279, 342, 720, 1268, 1311, 1644
Bsp13I	TCCGGA	1 cut(s)	637
Bsp1720I	GCTNAGC	1 cut(s)	598
Bsp19I	CCATGG	1 cut(s)	366
BspCNI	CTCAG	5 cut(s)	480, 583, 611, 616, 1287
BspEI	TCCGGA	1 cut(s)	637
BspFNI	CGCG	4 cut(s)	46, 132, 389, 1804
BspHI	TCATGA	1 cut(s)	1360
BspLI	GGNNCC	8 cut(s)	195, 225, 339, 347, 434, 718, 1665, 1706
BspMAI	CTGCAG	3 cut(s)	958, 984, 1419
BspPI	GGATC	5 cut(s)	122, 674, 1120, 1658, 1671
BspT107I	GGYRCC	1 cut(s)	1704
BspTI	CTTAAG	1 cut(s)	653
BsrFI	RCCGGY	2 cut(s)	58, 1701
BsrI	ACTGG	4 cut(s)	260, 434, 795, 929
BssAI	RCCGGY	2 cut(s)	58, 1701
BssECI	CCNNGG	2 cut(s)	341, 366
BssNI	GRCGYC	3 cut(s)	159, 519, 1383
BssT1I	CCWWGG	1 cut(s)	366
Bst2UI	CCWGG	4 cut(s)	350, 684, 1356, 1575
Bst4CI	ACNGT	6 cut(s)	615, 1048, 1246, 1465, 1471, 1853
BstACI	GRCGYC	3 cut(s)	159, 519, 1383
BstAFI	CTTAAG	1 cut(s)	653
BstBAI	YACGTR	1 cut(s)	1343
BstC8I	GCNNGC	8 cut(s)	450, 726, 820, 1008, 1077, 1278, 1307, 1654
BstDEI	CTNAG	6 cut(s)	467, 570, 598, 624, 1052, 1295
BstDSI	CCRYGG	1 cut(s)	366
BstF5I	GGATG	3 cut(s)	298, 902, 1033
BstFNI	CGCG	4 cut(s)	46, 132, 389, 1804
BstH2I	RGCGCY	2 cut(s)	412, 1503
BstHHI	GCGC	4 cut(s)	48, 389, 411, 1502
BstMAI	GTCTC	3 cut(s)	166, 1011, 1293
BstMCI	CGRYCG	4 cut(s)	112, 126, 130, 1450
BstNI	CCWGG	4 cut(s)	350, 684, 1356, 1575
BstNSI	RCATGY	2 cut(s)	1280, 1392
BstSCI	CCNGG	7 cut(s)	106, 341, 348, 682, 1354, 1378, 1573
BstSFI	CTRYAG	3 cut(s)	954, 980, 1415
BstSLI	GKGCMC	2 cut(s)	720, 1311
BstUI	CGCG	4 cut(s)	46, 132, 389, 1804
BstV1I	GCAGC	6 cut(s)	57, 986, 1054, 1401, 1610, 1643
BstV2I	GAAGAC	3 cut(s)	185, 491, 956
BstX2I	RGATCY	2 cut(s)	1490, 1663
BstXI	CCANNNNNNTGG	1 cut(s)	1574
BstYI	RGATCY	2 cut(s)	1490, 1663
BstZI	CGGCCG	2 cut(s)	109, 123
Bsu36I	CCTNAGG	1 cut(s)	467
BtgI	CCRYGG	1 cut(s)	366
BtgZI	GCGATG	1 cut(s)	1729
BtsCI	GGATG	3 cut(s)	298, 902, 1033
BtsI	GCAGTG	3 cut(s)	879, 977, 1644
BtsIMutI	CAGTG	5 cut(s)	879, 977, 1044, 1476, 1644
Cac8I	GCNNGC	8 cut(s)	450, 726, 820, 1008, 1077, 1278, 1307, 1654
CciI	TCATGA	1 cut(s)	1360
CfoI	GCGC	4 cut(s)	48, 389, 411, 1502
Cfr10I	RCCGGY	2 cut(s)	58, 1701
Cfr13I	GGNCC	4 cut(s)	223, 608, 716, 717
CseI	GACGC	2 cut(s)	1372, 1690
CsiI	ACCWGGT	1 cut(s)	1573
Csp6I	GTAC	6 cut(s)	247, 262, 765, 797, 1337, 1849
CspAI	ACCGGT	1 cut(s)	1701
CviAII	CATG	8 cut(s)	367, 415, 916, 1186, 1277, 1361, 1389, 1753
CviQI	GTAC	6 cut(s)	247, 262, 765, 797, 1337, 1849
DdeI	CTNAG	6 cut(s)	467, 570, 598, 624, 1052, 1295
DriI	GACNNNNNGTC	2 cut(s)	1244, 1453
EaeI	YGGCCR	4 cut(s)	109, 123, 421, 1758
EagI	CGGCCG	2 cut(s)	109, 123
Eam1105I	GACNNNNNGTC	2 cut(s)	1244, 1453
EciI	GGCGGA	2 cut(s)	182, 256
Ecl136II	GAGCTC	1 cut(s)	277
EclXI	CGGCCG	2 cut(s)	109, 123
Eco130I	CCWWGG	1 cut(s)	366
Eco147I	AGGCCT	1 cut(s)	806
Eco24I	GRGCYC	4 cut(s)	196, 279, 342, 720
Eco47I	GGWCC	2 cut(s)	223, 608
Eco52I	CGGCCG	2 cut(s)	109, 123
Eco53kI	GAGCTC	1 cut(s)	277
Eco57I	CTGAAG	4 cut(s)	524, 1604, 1737, 1803
Eco81I	CCTNAGG	1 cut(s)	467
Eco88I	CYCGRG	4 cut(s)	153, 278, 491, 719
EcoICRI	GAGCTC	1 cut(s)	277
EcoO109I	RGGNCCY	1 cut(s)	223
EcoRII	CCWGG	4 cut(s)	348, 682, 1354, 1573
EcoT14I	CCWWGG	1 cut(s)	366
EcoT38I	GRGCYC	4 cut(s)	196, 279, 342, 720
ErhI	CCWWGG	1 cut(s)	366
Esp3I	CGTCTC	2 cut(s)	166, 1293
FaeI	CATG	8 cut(s)	370, 418, 919, 1189, 1280, 1364, 1392, 1756
FaqI	GGGAC	1 cut(s)	170
FatI	CATG	8 cut(s)	366, 414, 915, 1185, 1276, 1360, 1388, 1752
FauI	CCCGC	2 cut(s)	1620, 1817
FbaI	TGATCA	1 cut(s)	694
FokI	GGATG	3 cut(s)	305, 909, 1040
FriOI	GRGCYC	4 cut(s)	196, 279, 342, 720
FspBI	CTAG	2 cut(s)	777, 906
GlaI	GCGC	4 cut(s)	47, 388, 410, 1501
GsaI	CCCAGC	1 cut(s)	1590
GsuI	CTGGAG	2 cut(s)	398, 1623
HaeII	RGCGCY	2 cut(s)	412, 1503
HgaI	GACGC	2 cut(s)	1372, 1690
HhaI	GCGC	4 cut(s)	48, 389, 411, 1502
Hin1I	GRCGYC	3 cut(s)	159, 519, 1383
Hin1II	CATG	8 cut(s)	370, 418, 919, 1189, 1280, 1364, 1392, 1756
Hin6I	GCGC	4 cut(s)	46, 387, 409, 1500
HinP1I	GCGC	4 cut(s)	46, 387, 409, 1500
HincII	GTYRAC	2 cut(s)	253, 1552
HindII	GTYRAC	2 cut(s)	253, 1552
HinfI	GANTC	6 cut(s)	80, 298, 909, 1190, 1231, 1454
HphI	GGTGA	4 cut(s)	229, 1240, 1406, 1791
Hpy166II	GTNNAC	6 cut(s)	253, 312, 567, 1309, 1552, 1799
Hpy188III	TCNNGA	9 cut(s)	155, 290, 401, 511, 638, 770, 857, 1361, 1531
Hpy8I	GTNNAC	6 cut(s)	253, 312, 567, 1309, 1552, 1799
Hpy99I	CGWCG	3 cut(s)	40, 521, 524
HpyCH4III	ACNGT	6 cut(s)	615, 1048, 1246, 1465, 1471, 1853
HpyCH4IV	ACGT	4 cut(s)	159, 249, 519, 1342
HpyCH4V	TGCA	8 cut(s)	475, 707, 833, 956, 982, 1309, 1417, 1659
HpyF3I	CTNAG	6 cut(s)	467, 570, 598, 624, 1052, 1295
HpySE526I	ACGT	4 cut(s)	159, 249, 519, 1342
Hsp92I	GRCGYC	3 cut(s)	159, 519, 1383
Hsp92II	CATG	8 cut(s)	370, 418, 919, 1189, 1280, 1364, 1392, 1756
HspAI	GCGC	4 cut(s)	46, 387, 409, 1500
Kpn2I	TCCGGA	1 cut(s)	637
Ksp22I	TGATCA	1 cut(s)	694
LmnI	GCTCC	8 cut(s)	199, 274, 337, 373, 438, 640, 1263, 1369
Lsp1109I	GCAGC	6 cut(s)	57, 986, 1054, 1401, 1610, 1643
LweI	GCATC	2 cut(s)	1818, 1831
MabI	ACCWGGT	1 cut(s)	1573
MaeI	CTAG	2 cut(s)	777, 906
MaeII	ACGT	4 cut(s)	159, 249, 519, 1342
MaeIII	GTNAC	5 cut(s)	786, 1384, 1394, 1465, 1480
MflI	RGATCY	2 cut(s)	1490, 1663
MhlI	GDGCHC	7 cut(s)	196, 279, 342, 720, 1268, 1311, 1644
MluCI	AATT	7 cut(s)	588, 690, 862, 888, 1205, 1312, 1773
MlyI	GAGTC	2 cut(s)	307, 1463
MmeI	TCCRAC	2 cut(s)	59, 565
MroI	TCCGGA	1 cut(s)	637
MroXI	GAANNNNTTC	1 cut(s)	806
MseI	TTAA	5 cut(s)	537, 654, 996, 1557, 1855
MspA1I	CMGCKG	4 cut(s)	528, 1180, 1601, 1656
MspCI	CTTAAG	1 cut(s)	653
MspR9I	CCNGG	7 cut(s)	108, 343, 350, 684, 1356, 1380, 1575
Mva1269I	GAATGC	1 cut(s)	1416
MvaI	CCWGG	4 cut(s)	350, 684, 1356, 1575
MvnI	CGCG	4 cut(s)	46, 132, 389, 1804
NciI	CCSGG	3 cut(s)	108, 343, 1380
NcoI	CCATGG	1 cut(s)	366
NlaIII	CATG	8 cut(s)	370, 418, 919, 1189, 1280, 1364, 1392, 1756
NlaIV	GGNNCC	8 cut(s)	195, 225, 339, 347, 434, 718, 1665, 1706
NmeAIII	GCCGAG	1 cut(s)	143
NmuCI	GTSAC	3 cut(s)	1384, 1394, 1480
NspI	RCATGY	2 cut(s)	1280, 1392
PaeI	GCATGC	1 cut(s)	1280
PaeR7I	CTCGAG	1 cut(s)	491
PagI	TCATGA	1 cut(s)	1360
PceI	AGGCCT	1 cut(s)	806
PciI	ACATGT	1 cut(s)	1388
PctI	GAATGC	1 cut(s)	1416
PdmI	GAANNNNTTC	1 cut(s)	806
PfeI	GAWTC	4 cut(s)	80, 909, 1190, 1231
PflFI	GACNNNGTC	1 cut(s)	521
PfoI	TCCNGGA	1 cut(s)	682
PinAI	ACCGGT	1 cut(s)	1701
Ple19I	CGATCG	1 cut(s)	130
PleI	GAGTC	2 cut(s)	306, 1462
PpsI	GAGTC	2 cut(s)	306, 1462
Ppu21I	YACGTR	1 cut(s)	1343
PpuMI	RGGWCCY	1 cut(s)	223
PscI	ACATGT	1 cut(s)	1388
Psp124BI	GAGCTC	1 cut(s)	279
Psp5II	RGGWCCY	1 cut(s)	223
Psp6I	CCWGG	4 cut(s)	348, 682, 1354, 1573
PspFI	CCCAGC	1 cut(s)	1586
PspGI	CCWGG	4 cut(s)	348, 682, 1354, 1573
PspN4I	GGNNCC	8 cut(s)	195, 225, 339, 347, 434, 718, 1665, 1706
PspOMI	GGGCCC	1 cut(s)	716
PspPI	GGNCC	4 cut(s)	223, 608, 716, 717
PspPPI	RGGWCCY	1 cut(s)	223
PspXI	VCTCGAGB	1 cut(s)	491
PstI	CTGCAG	3 cut(s)	958, 984, 1419
PsuI	RGATCY	2 cut(s)	1490, 1663
PsyI	GACNNNGTC	1 cut(s)	521
PvuI	CGATCG	1 cut(s)	130
PvuII	CAGCTG	3 cut(s)	1180, 1601, 1656
RsaI	GTAC	6 cut(s)	248, 263, 766, 798, 1338, 1850
RsaNI	GTAC	6 cut(s)	247, 262, 765, 797, 1337, 1849
SacI	GAGCTC	1 cut(s)	279
SaqAI	TTAA	5 cut(s)	537, 654, 996, 1557, 1855
Sau96I	GGNCC	4 cut(s)	223, 608, 716, 717
ScaI	AGTACT	1 cut(s)	1338
SchI	GAGTC	2 cut(s)	307, 1463
ScrFI	CCNGG	7 cut(s)	108, 343, 350, 684, 1356, 1380, 1575
SduI	GDGCHC	7 cut(s)	196, 279, 342, 720, 1268, 1311, 1644
SexAI	ACCWGGT	1 cut(s)	1573
SfaNI	GCATC	2 cut(s)	1818, 1831
SfcI	CTRYAG	3 cut(s)	954, 980, 1415
Sfr274I	CTCGAG	1 cut(s)	491
SinI	GGWCC	2 cut(s)	223, 608
SlaI	CTCGAG	1 cut(s)	491
SmlI	CTYRAG	3 cut(s)	491, 653, 855
SmoI	CTYRAG	3 cut(s)	491, 653, 855
SphI	GCATGC	1 cut(s)	1280
Sse9I	AATT	7 cut(s)	588, 690, 862, 888, 1205, 1312, 1773
SseBI	AGGCCT	1 cut(s)	806
SspMI	CTAG	2 cut(s)	777, 906
SstI	GAGCTC	1 cut(s)	279
StuI	AGGCCT	1 cut(s)	806
StyD4I	CCNGG	7 cut(s)	106, 341, 348, 682, 1354, 1378, 1573
StyI	CCWWGG	1 cut(s)	366
TaaI	ACNGT	6 cut(s)	615, 1048, 1246, 1465, 1471, 1853
TaiI	ACGT	4 cut(s)	162, 252, 522, 1345
TaqI	TCGA	5 cut(s)	492, 512, 1457, 1671, 1839
TaqII	GACCGA	1 cut(s)	1464
TasI	AATT	7 cut(s)	588, 690, 862, 888, 1205, 1312, 1773
TatI	WGTACW	2 cut(s)	796, 1336
TauI	GCSGC	6 cut(s)	135, 356, 414, 544, 1354, 1760
TfiI	GAWTC	4 cut(s)	80, 909, 1190, 1231
Tru1I	TTAA	5 cut(s)	537, 654, 996, 1557, 1855
Tru9I	TTAA	5 cut(s)	537, 654, 996, 1557, 1855
TscAI	CASTG	5 cut(s)	886, 984, 1051, 1476, 1644
TseFI	GTSAC	3 cut(s)	1384, 1394, 1480
TseI	GCWGC	6 cut(s)	70, 974, 1067, 1414, 1598, 1656
Tsp45I	GTSAC	3 cut(s)	1384, 1394, 1480
TspDTI	ATGAA	2 cut(s)	855, 1318
TspGWI	ACGGA	2 cut(s)	329, 800
TspRI	CASTG	5 cut(s)	886, 984, 1051, 1476, 1644
Tth111I	GACNNNGTC	1 cut(s)	521
Vha464I	CTTAAG	1 cut(s)	653
VneI	GTGCAC	1 cut(s)	1307
VpaK11BI	GGWCC	2 cut(s)	223, 608
XapI	RAATTY	2 cut(s)	862, 1205
XceI	RCATGY	2 cut(s)	1280, 1392
XcmI	CCANNNNNNNNNTGG	3 cut(s)	234, 373, 1362
XhoI	CTCGAG	1 cut(s)	491
XmnI	GAANNNNTTC	1 cut(s)	806
XspI	CTAG	2 cut(s)	777, 906
ZraI	GACGTC	2 cut(s)	160, 520
ZrmI	AGTACT	1 cut(s)	1338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.