Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003547

Physical Location & Seq

Genomic Coordinates Forward (+)

9195677 .. 9201093

Gene Structure 5417 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001012

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGGCTTCAATTTCCGGCATTTGTTCCTCTTCTCCGACTCTGCAACCGCGGCTGAGACCGGCGGCGAGTATATTACGTAGAGACTCTGTTTCGTTTCCGACGGCCCGGCCTAGGCCCGGCGATCGGAGCTTGTTCGCTCAGTCGGTGGCGCGGGAGATCAAGGCCGAGTTGGCCAAGGCTGACGGCGATGGCGCTCTGATTAAGGAGAGGAAGAAGGTTCTGGAGAAGGATCCGCGGGCGTTGTGGCGGAGGTACGTGGACTGGTTGTACCAGCACAAGGAGCTAGGGTTGTATTTGGATGTGAGTCGGGTCGGGTTTACGGACGAGTTCGTCGCGGAAATGGAGCCCCGGTTCCAGGCGGCGTTTAAGGCCATGGAGGACCTGGAGAAAGGCGCGATTGCGAATCCCGATGAGGGGCGGATGGTCGGGCATTACTGGCTGAGAGACCCTAAGCGGGCGCCGAAACCCGTACTCACGGCTCAGATTGAGAACACGCTCGAGGCGGTGCTTAAGTTCTCCGGCGACGTCGTCAGCGGTAAGATTAAGCCGCCAGCTTCTTCGGAGGGTCGTTTTACTCATGTTCTTTCGGTTGGAATCGGAGGCTCGGCACTTGGACCACAGTTTGTTGCTGAGGCATTGGCGCCTGATGTTCCTCCTCTTAAGATACGATTCATTGACAATACCGATCCAGCAGGAATTGATCATCAGATTGCACAGCTCGGTGAAGAACTGGCTTCTACTCTTGTAATTGTGATTTCAAAGAGTGGAGGTACTCCTGAAACTAGAAATGGTTTACTCGAAGTACAGAAGGCCTTTCGTGAGGCTGGCCTGAATTTCGCAAAACAGGGTGTGGCTATAACTCAAGAAAATTCATTATTAGACAACACTGCTAGAATTGAGGGCTGGTTAGCTAGATTCCCCATGTTTGACTGGGTTGGTGGAAGAACATCTGAAATGTCTGCGGTTGGTCTTCTTCCAGCAGCGCTTCAGGGAATTGACATTAAAGAAATGCTTGATGGTGGAAAGTTGATGGATGAGGCTAATAGAACCACTGTGGTTAAGAATAATCCTGCCGCCTTGCTTGCTTTATGCTGGTATTGGGCTACTGATGGAGTGGGATCCAAGGACATGGTTGTTCTTCCTTACAAGGACAGCCTATTATTATTTAGTCGGTATTTGCAGCAGTTGGTCATGGAATCTATTGGGAAAGAATTTGACCTTGATGGTAATCGGGTGAATCAAGGACTTACAGTGTATGGAAATAAAGGGAGCACGGATCAGCATGCATACATCCAACAACTGAGAGATGGCGTACACAATTTCTTTGTGACATTCATCGAAGTACTACAAGATAGACCTCCTGGTCATGATTGGGAGCTTGAACCAGGTGTCACATGTGGTGACTACCTATTTGGAATGCTACAGGGAACACGATCGGCTCTTTATTCTAATGACCGAGAGTCGATTACAGTTTCTGTGGAAGAAGTAACACCTAGATCTGTAGGTGCTCTTATAGCACTTTATGAGCGAGCGGTTGGGATTTACGCCTCACTTGTCAACATTAATGCTTACCATCAACCTGGTGTGGAGGCCGGGAAGAAAGCAGCCGGAGAAGTATTAGCCCTTCAGAAACGGGTTTTATCCGTACTTAATGAGGCCAGCTGTAAAGAACCTGTTGAACCATTGACACTAGATGAAGTAGCCGATCGGTGCCACGCTCCTGAAGATATTGAAATGATTTACAAGATCATCGCTCACATGGCGGCAAATGACAGAGCTCTTATTGCTGAAGGCAGTTGCGGTTCACCACGGAGCGTCAAAGTTTTCCTCGGCGAGTGTAATGTGGAGGCGTTGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009909 GO:0009911 GO:0009941 GO:0009987 GO:0016853 GO:0016860 GO:0016861 GO:0031967 GO:0031975 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0048831 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0065007 GO:0071704 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

PGI

Protein Analysis

620

Amino Acids

67.77

Weight (kDa)

5.46

Isoelectric Point (pI)

39.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGI	PF00342	124 - 451	2.2e-48	Phosphoglucose isomerase
PGI	PF00342	481 - 541	1.9e-09	Phosphoglucose isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003547)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24620 AT4G24620
fragaria_vesca	FvH4_5g35680 FvH4_5g35680
malus_domestica	MD08G1194100.v1.1 MD15G1382200.v1.1
prunus_persica	Prupe.1G528400_v2.0.a1 Prupe.1G528400_v2.0.a1
pyrus_communis	pycom15g34220
rosa_chinensis	RchiOBHm_Chr7g0237251 RchiOBHm_Chr7g0237271 RchiOBHm_Chr7g0237281 RchiOBHm_Chr7g0237311 RchiOBHm_Chr7g0237321
rosa_laevigata	RLG00000001012 RLG00000001018
rosa_multiflora	Rmu_sc0002923.1_g000043 Rmu_sc0002923.1_g000049 Rmu_sc0006438.1_g000001 Rmu_sc0006438.1_g000006 Rmu_sc0006438.1_g000007
rosa_roxburghii	Rroxscaffold_3G00224320
rosa_rugosa	Rorug07G0302600 Rorug07G0302700
rosa_samantha	Rh7AG459300 Rh7AG459500 Rh7AG459600 Rh7AG459700 Rh7AG459800 Rh7BG429700 Rh7CG477100 Rh7DG446400
rosa_wichuraiana	Rw0G003870 Rw7G038060 Rw7G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	329, 1362
AatII	GACGTC	1 cut(s)	528
AccB1I	GGYRCC	3 cut(s)	457, 640, 1710
AccBSI	CCGCTC	1 cut(s)	1532
AccII	CGCG	5 cut(s)	49, 151, 235, 335, 395
AclWI	GGATC	6 cut(s)	224, 237, 680, 1113, 1126, 1284
AcoI	YGGCCR	1 cut(s)	171
AcsI	RAATTY	3 cut(s)	832, 868, 1211
AcuI	CTGAAG	4 cut(s)	971, 1610, 1743, 1809
AcyI	GRCGYC	3 cut(s)	458, 525, 641
AfaI	GTAC	8 cut(s)	254, 269, 471, 772, 804, 1314, 1344, 1647
AfeI	AGCGCT	1 cut(s)	984
AfiI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 277, 355, 413, 414, 454
AflII	CTTAAG	2 cut(s)	509, 659
AflIII	ACRYGT	1 cut(s)	1394
AgsI	TTSAA	5 cut(s)	9, 759, 1382, 1679, 1733
AhdI	GACNNNNNGTC	1 cut(s)	1459
AjnI	CCWGG	5 cut(s)	354, 381, 1360, 1384, 1579
AloI	GAACNNNNNNTCC	2 cut(s)	335, 367
AluBI	AGCT	8 cut(s)	129, 283, 554, 718, 911, 1378, 1662, 1778
AluI	AGCT	8 cut(s)	129, 283, 554, 718, 911, 1378, 1662, 1778
Alw21I	GWGCWC	3 cut(s)	1274, 1510, 1780
Alw26I	GTCTC	3 cut(s)	49, 75, 438
AlwI	GGATC	6 cut(s)	224, 237, 680, 1113, 1126, 1284
AlwNI	CAGNNNCTG	1 cut(s)	1475
Ama87I	CYCGRG	1 cut(s)	497
Aor51HI	AGCGCT	1 cut(s)	984
ApeKI	GCWGC	3 cut(s)	980, 1180, 1604
ApoI	RAATTY	3 cut(s)	832, 868, 1211
ArsI	GACNNNNNNTTYG	2 cut(s)	1394, 1426
AseI	ATTAAT	1 cut(s)	1563
AspA2I	CCTAGG	1 cut(s)	110
AspLEI	GCGC	6 cut(s)	151, 194, 395, 460, 643, 985
AspS9I	GGNCC	4 cut(s)	103, 114, 379, 614
AsuC2I	CCSGG	4 cut(s)	106, 117, 349, 1594
AsuHPI	GGTGA	4 cut(s)	734, 1246, 1412, 1797
AvaI	CYCGRG	1 cut(s)	497
AvaII	GGWCC	2 cut(s)	379, 614
AvrII	CCTAGG	1 cut(s)	110
BalI	TGGCCA	1 cut(s)	173
BamHI	GGATCC	2 cut(s)	229, 1118
BanI	GGYRCC	3 cut(s)	457, 640, 1710
BanII	GRGCYC	2 cut(s)	348, 1780
BarI	GAAGNNNNNNTAC	2 cut(s)	1608, 1640
BbsI	GAAGAC	1 cut(s)	962
Bbv12I	GWGCWC	3 cut(s)	1274, 1510, 1780
BbvCI	CCTCAGC	1 cut(s)	630
BbvI	GCAGC	3 cut(s)	992, 1192, 1616
BccI	CCATC	8 cut(s)	182, 415, 1010, 1024, 1103, 1217, 1301, 1581
BceAI	ACGGC	3 cut(s)	117, 199, 492
BciT130I	CCWGG	5 cut(s)	356, 383, 1362, 1386, 1581
BclI	TGATCA	1 cut(s)	700
BcnI	CCSGG	4 cut(s)	106, 117, 349, 1594
BcoDI	GTCTC	3 cut(s)	49, 75, 438
BfaI	CTAG	7 cut(s)	111, 284, 783, 891, 912, 1494, 1691
BfmI	CTRYAG	2 cut(s)	1421, 1500
BfoI	RGCGCY	4 cut(s)	195, 461, 644, 986
BfrI	CTTAAG	2 cut(s)	509, 659
BglI	GCCNNNNNGGC	1 cut(s)	170
BglII	AGATCT	1 cut(s)	1496
BisI	GCNGC	9 cut(s)	50, 63, 360, 548, 981, 1074, 1181, 1605, 1764
BlnI	CCTAGG	1 cut(s)	110
BlsI	GCNGC	9 cut(s)	51, 64, 361, 549, 982, 1075, 1182, 1606, 1765
BmcAI	AGTACT	1 cut(s)	1344
Bme1390I	CCNGG	9 cut(s)	106, 117, 349, 356, 383, 1362, 1386, 1581, 1594
Bme18I	GGWCC	2 cut(s)	379, 614
BmeRI	GACNNNNNGTC	1 cut(s)	1459
BmeT110I	CYCGRG	1 cut(s)	497
BmgT120I	GGNCC	4 cut(s)	103, 114, 379, 614
BmiI	GGNNCC	7 cut(s)	231, 345, 353, 459, 642, 1120, 1712
BmrFI	CCNGG	9 cut(s)	106, 117, 349, 356, 383, 1362, 1386, 1581, 1594
BmrI	ACTGGG	1 cut(s)	940
BmuI	ACTGGG	1 cut(s)	940
BpiI	GAAGAC	1 cut(s)	962
BpmI	CTGGAG	2 cut(s)	242, 404
Bpu10I	CCTNAGC	2 cut(s)	450, 630
BpuEI	CTTGAG	1 cut(s)	846
BpuMI	CCSGG	4 cut(s)	106, 117, 349, 1594
BsaAI	YACGTR	2 cut(s)	77, 256
BsaBI	GATNNNNATC	1 cut(s)	1227
BsaHI	GRCGYC	3 cut(s)	458, 525, 641
BsaI	GGTCTC	2 cut(s)	49, 438
BsaJI	CCNNGG	9 cut(s)	47, 110, 174, 233, 347, 372, 1122, 1808, 1828
BsaXI	ACNNNNNCTCC	5 cut(s)	272, 302, 335, 365, 1838
Bsc4I	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 277, 355, 413, 414, 454
Bse118I	RCCGGY	1 cut(s)	58
Bse1I	ACTGG	4 cut(s)	266, 440, 735, 935
Bse8I	GATNNNNATC	1 cut(s)	1227
BseBI	CCWGG	5 cut(s)	356, 383, 1362, 1386, 1581
BseDI	CCNNGG	9 cut(s)	47, 110, 174, 233, 347, 372, 1122, 1808, 1828
BseGI	GGATG	4 cut(s)	304, 426, 1039, 1290
BseJI	GATNNNNATC	1 cut(s)	1227
BseLI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 277, 355, 413, 414, 454
BseMII	CTCAG	6 cut(s)	44, 152, 431, 494, 621, 1292
BseNI	ACTGG	4 cut(s)	266, 440, 735, 935
BseRI	GAGGAG	1 cut(s)	645
BseXI	GCAGC	3 cut(s)	992, 1192, 1616
Bsh1236I	CGCG	5 cut(s)	49, 151, 235, 335, 395
Bsh1285I	CGRYCG	3 cut(s)	124, 1436, 1708
BshNI	GGYRCC	3 cut(s)	457, 640, 1710
BsiEI	CGRYCG	3 cut(s)	124, 1436, 1708
BsiHKAI	GWGCWC	3 cut(s)	1274, 1510, 1780
BsiHKCI	CYCGRG	1 cut(s)	497
BsiSI	CCGG	8 cut(s)	15, 59, 106, 117, 349, 519, 1593, 1608
BslI	CCNNNNNNNGG	8 cut(s)	111, 116, 123, 277, 355, 413, 414, 454
BsmAI	GTCTC	3 cut(s)	49, 75, 438
BsmI	GAATGC	1 cut(s)	1422
Bso31I	GGTCTC	2 cut(s)	49, 438
BsoBI	CYCGRG	1 cut(s)	497
Bsp1286I	GDGCHC	4 cut(s)	348, 1274, 1510, 1780
Bsp19I	CCATGG	1 cut(s)	372
BspCNI	CTCAG	6 cut(s)	45, 151, 432, 493, 622, 1293
BspFNI	CGCG	5 cut(s)	49, 151, 235, 335, 395
BspHI	TCATGA	1 cut(s)	1366
BspLI	GGNNCC	7 cut(s)	231, 345, 353, 459, 642, 1120, 1712
BspPI	GGATC	6 cut(s)	224, 237, 680, 1113, 1126, 1284
BspT107I	GGYRCC	3 cut(s)	457, 640, 1710
BspTI	CTTAAG	2 cut(s)	509, 659
BspTNI	GGTCTC	2 cut(s)	49, 438
BsrBI	CCGCTC	1 cut(s)	1532
BsrFI	RCCGGY	1 cut(s)	58
BsrI	ACTGG	4 cut(s)	266, 440, 735, 935
BssAI	RCCGGY	1 cut(s)	58
BssECI	CCNNGG	9 cut(s)	47, 110, 174, 233, 347, 372, 1122, 1808, 1828
BssNI	GRCGYC	3 cut(s)	458, 525, 641
BssT1I	CCWWGG	4 cut(s)	110, 174, 372, 1122
Bst2UI	CCWGG	5 cut(s)	356, 383, 1362, 1386, 1581
Bst4CI	ACNGT	4 cut(s)	621, 1054, 1252, 1471
Bst6I	CTCTTC	1 cut(s)	34
BstACI	GRCGYC	3 cut(s)	458, 525, 641
BstAFI	CTTAAG	2 cut(s)	509, 659
BstBAI	YACGTR	2 cut(s)	77, 256
BstC8I	GCNNGC	8 cut(s)	237, 456, 552, 826, 1083, 1284, 1530, 1660
BstDEI	CTNAG	7 cut(s)	53, 138, 440, 450, 480, 630, 1301
BstDSI	CCRYGG	4 cut(s)	47, 233, 372, 1808
BstF5I	GGATG	4 cut(s)	304, 426, 1039, 1290
BstFNI	CGCG	5 cut(s)	49, 151, 235, 335, 395
BstH2I	RGCGCY	4 cut(s)	195, 461, 644, 986
BstHHI	GCGC	6 cut(s)	151, 194, 395, 460, 643, 985
BstMAI	GTCTC	3 cut(s)	49, 75, 438
BstMCI	CGRYCG	3 cut(s)	124, 1436, 1708
BstNI	CCWGG	5 cut(s)	356, 383, 1362, 1386, 1581
BstNSI	RCATGY	2 cut(s)	1286, 1398
BstSCI	CCNGG	9 cut(s)	104, 115, 347, 354, 381, 1360, 1384, 1579, 1592
BstSFI	CTRYAG	2 cut(s)	1421, 1500
BstSNI	TACGTA	1 cut(s)	77
BstUI	CGCG	5 cut(s)	49, 151, 235, 335, 395
BstV1I	GCAGC	3 cut(s)	992, 1192, 1616
BstV2I	GAAGAC	1 cut(s)	962
BstX2I	RGATCY	3 cut(s)	229, 1118, 1496
BstXI	CCANNNNNNTGG	2 cut(s)	1129, 1580
BstYI	RGATCY	3 cut(s)	229, 1118, 1496
BtgI	CCRYGG	4 cut(s)	47, 233, 372, 1808
BtgZI	GCGATG	2 cut(s)	201, 1735
BtsCI	GGATG	4 cut(s)	304, 426, 1039, 1290
BtsI	GCAGTG	1 cut(s)	885
BtsIMutI	CAGTG	3 cut(s)	885, 1050, 1257
Cac8I	GCNNGC	8 cut(s)	237, 456, 552, 826, 1083, 1284, 1530, 1660
CaiI	CAGNNNCTG	1 cut(s)	1475
CciI	TCATGA	1 cut(s)	1366
CfoI	GCGC	6 cut(s)	151, 194, 395, 460, 643, 985
Cfr10I	RCCGGY	1 cut(s)	58
Cfr13I	GGNCC	4 cut(s)	103, 114, 379, 614
Cfr42I	CCGCGG	2 cut(s)	50, 236
CseI	GACGC	1 cut(s)	1804
CsiI	ACCWGGT	2 cut(s)	1384, 1579
Csp6I	GTAC	8 cut(s)	253, 268, 470, 771, 803, 1313, 1343, 1646
CviAII	CATG	9 cut(s)	373, 578, 922, 1129, 1192, 1283, 1367, 1395, 1759
CviQI	GTAC	8 cut(s)	253, 268, 470, 771, 803, 1313, 1343, 1646
DdeI	CTNAG	7 cut(s)	53, 138, 440, 450, 480, 630, 1301
DinI	GGCGCC	2 cut(s)	459, 642
DrdI	GACNNNNNNGTC	2 cut(s)	329, 1362
DriI	GACNNNNNGTC	1 cut(s)	1459
DseDI	GACNNNNNNGTC	2 cut(s)	329, 1362
EaeI	YGGCCR	1 cut(s)	171
Eam1104I	CTCTTC	1 cut(s)	34
Eam1105I	GACNNNNNGTC	1 cut(s)	1459
EarI	CTCTTC	1 cut(s)	34
EciI	GGCGGA	2 cut(s)	262, 433
Ecl136II	GAGCTC	1 cut(s)	1778
Eco105I	TACGTA	1 cut(s)	77
Eco130I	CCWWGG	4 cut(s)	110, 174, 372, 1122
Eco147I	AGGCCT	1 cut(s)	812
Eco24I	GRGCYC	2 cut(s)	348, 1780
Eco31I	GGTCTC	2 cut(s)	49, 438
Eco47I	GGWCC	2 cut(s)	379, 614
Eco47III	AGCGCT	1 cut(s)	984
Eco53kI	GAGCTC	1 cut(s)	1778
Eco57I	CTGAAG	4 cut(s)	971, 1610, 1743, 1809
Eco88I	CYCGRG	1 cut(s)	497
EcoICRI	GAGCTC	1 cut(s)	1778
EcoO109I	RGGNCCY	1 cut(s)	379
EcoRII	CCWGG	5 cut(s)	354, 381, 1360, 1384, 1579
EcoT14I	CCWWGG	4 cut(s)	110, 174, 372, 1122
EcoT22I	ATGCAT	1 cut(s)	1288
EcoT38I	GRGCYC	2 cut(s)	348, 1780
EgeI	GGCGCC	2 cut(s)	459, 642
EheI	GGCGCC	2 cut(s)	459, 642
ErhI	CCWWGG	4 cut(s)	110, 174, 372, 1122
FaeI	CATG	9 cut(s)	376, 581, 925, 1132, 1195, 1286, 1370, 1398, 1762
FalI	AAGNNNNNCTT	2 cut(s)	996, 1028
FatI	CATG	9 cut(s)	372, 577, 921, 1128, 1191, 1282, 1366, 1394, 1758
FauI	CCCGC	3 cut(s)	144, 228, 447
FbaI	TGATCA	1 cut(s)	700
Fnu4HI	GCNGC	9 cut(s)	50, 63, 360, 548, 981, 1074, 1181, 1605, 1764
FokI	GGATG	4 cut(s)	311, 433, 1046, 1277
FriOI	GRGCYC	2 cut(s)	348, 1780
Fsp4HI	GCNGC	9 cut(s)	50, 63, 360, 548, 981, 1074, 1181, 1605, 1764
FspBI	CTAG	7 cut(s)	111, 284, 783, 891, 912, 1494, 1691
GlaI	GCGC	6 cut(s)	150, 193, 394, 459, 642, 984
GluI	GCNGC	9 cut(s)	50, 63, 360, 548, 981, 1074, 1181, 1605, 1764
GsuI	CTGGAG	2 cut(s)	242, 404
HaeII	RGCGCY	4 cut(s)	195, 461, 644, 986
HapII	CCGG	8 cut(s)	15, 59, 106, 117, 349, 519, 1593, 1608
HgaI	GACGC	1 cut(s)	1804
HhaI	GCGC	6 cut(s)	151, 194, 395, 460, 643, 985
Hin1I	GRCGYC	3 cut(s)	458, 525, 641
Hin1II	CATG	9 cut(s)	376, 581, 925, 1132, 1195, 1286, 1370, 1398, 1762
Hin6I	GCGC	6 cut(s)	149, 192, 393, 458, 641, 983
HinP1I	GCGC	6 cut(s)	149, 192, 393, 458, 641, 983
HincII	GTYRAC	1 cut(s)	1558
HindII	GTYRAC	1 cut(s)	1558
HpaII	CCGG	8 cut(s)	15, 59, 106, 117, 349, 519, 1593, 1608
HphI	GGTGA	4 cut(s)	734, 1246, 1412, 1797
Hpy166II	GTNNAC	6 cut(s)	259, 318, 794, 1315, 1558, 1805
Hpy188III	TCNNGA	7 cut(s)	221, 407, 776, 818, 863, 1367, 1721
Hpy8I	GTNNAC	6 cut(s)	259, 318, 794, 1315, 1558, 1805
Hpy99I	CGWCG	4 cut(s)	103, 335, 527, 530
HpyAV	CCTTC	5 cut(s)	208, 220, 802, 1634, 1784
HpyCH4III	ACNGT	4 cut(s)	621, 1054, 1252, 1471
HpyCH4IV	ACGT	3 cut(s)	76, 255, 525
HpyCH4V	TGCA	4 cut(s)	43, 713, 1180, 1286
HpyF3I	CTNAG	7 cut(s)	53, 138, 440, 450, 480, 630, 1301
HpySE526I	ACGT	3 cut(s)	76, 255, 525
Hsp92I	GRCGYC	3 cut(s)	458, 525, 641
Hsp92II	CATG	9 cut(s)	376, 581, 925, 1132, 1195, 1286, 1370, 1398, 1762
HspAI	GCGC	6 cut(s)	149, 192, 393, 458, 641, 983
KasI	GGCGCC	2 cut(s)	457, 640
Ksp22I	TGATCA	1 cut(s)	700
KspI	CCGCGG	2 cut(s)	50, 236
LmnI	GCTCC	7 cut(s)	126, 280, 343, 1269, 1375, 1723, 1812
Lsp1109I	GCAGC	3 cut(s)	992, 1192, 1616
MabI	ACCWGGT	2 cut(s)	1384, 1579
MaeI	CTAG	7 cut(s)	111, 284, 783, 891, 912, 1494, 1691
MaeII	ACGT	3 cut(s)	76, 255, 525
MaeIII	GTNAC	4 cut(s)	1327, 1390, 1400, 1486
MbiI	CCGCTC	1 cut(s)	1532
MflI	RGATCY	3 cut(s)	229, 1118, 1496
MhlI	GDGCHC	4 cut(s)	348, 1274, 1510, 1780
MlsI	TGGCCA	1 cut(s)	173
MluCI	AATT	9 cut(s)	9, 696, 747, 832, 868, 894, 993, 1211, 1318
MluNI	TGGCCA	1 cut(s)	173
Mly113I	GGCGCC	2 cut(s)	458, 641
MlyI	GAGTC	4 cut(s)	31, 77, 313, 1469
MmeI	TCCRAC	4 cut(s)	59, 122, 571, 1318
Mox20I	TGGCCA	1 cut(s)	173
Mph1103I	ATGCAT	1 cut(s)	1288
MscI	TGGCCA	1 cut(s)	173
MseI	TTAA	9 cut(s)	201, 366, 510, 543, 660, 1002, 1059, 1563, 1650
Msp20I	TGGCCA	1 cut(s)	173
MspA1I	CMGCKG	4 cut(s)	49, 235, 534, 1662
MspCI	CTTAAG	2 cut(s)	509, 659
MspI	CCGG	8 cut(s)	15, 59, 106, 117, 349, 519, 1593, 1608
MspR9I	CCNGG	9 cut(s)	106, 117, 349, 356, 383, 1362, 1386, 1581, 1594
Mva1269I	GAATGC	1 cut(s)	1422
MvaI	CCWGG	5 cut(s)	356, 383, 1362, 1386, 1581
MvnI	CGCG	5 cut(s)	49, 151, 235, 335, 395
NarI	GGCGCC	2 cut(s)	458, 641
NciI	CCSGG	4 cut(s)	106, 117, 349, 1594
NcoI	CCATGG	1 cut(s)	372
NlaIII	CATG	9 cut(s)	376, 581, 925, 1132, 1195, 1286, 1370, 1398, 1762
NlaIV	GGNNCC	7 cut(s)	231, 345, 353, 459, 642, 1120, 1712
NmeAIII	GCCGAG	3 cut(s)	190, 584, 1809
NmuCI	GTSAC	3 cut(s)	1327, 1390, 1400
NsiI	ATGCAT	1 cut(s)	1288
NspI	RCATGY	2 cut(s)	1286, 1398
PaeI	GCATGC	1 cut(s)	1286
PaeR7I	CTCGAG	1 cut(s)	497
PagI	TCATGA	1 cut(s)	1366
PceI	AGGCCT	1 cut(s)	812
PciI	ACATGT	1 cut(s)	1394
PctI	GAATGC	1 cut(s)	1422
PfeI	GAWTC	6 cut(s)	403, 594, 669, 915, 1196, 1237
PflFI	GACNNNGTC	1 cut(s)	527
PkrI	GCNGC	9 cut(s)	51, 64, 361, 549, 982, 1075, 1182, 1606, 1765
Ple19I	CGATCG	3 cut(s)	124, 1436, 1708
PleI	GAGTC	4 cut(s)	31, 77, 312, 1468
PluTI	GGCGCC	2 cut(s)	461, 644
PpsI	GAGTC	4 cut(s)	31, 77, 312, 1468
Ppu21I	YACGTR	2 cut(s)	77, 256
PpuMI	RGGWCCY	1 cut(s)	379
PscI	ACATGT	1 cut(s)	1394
PshBI	ATTAAT	1 cut(s)	1563
Psp124BI	GAGCTC	1 cut(s)	1780
Psp5II	RGGWCCY	1 cut(s)	379
Psp6I	CCWGG	5 cut(s)	354, 381, 1360, 1384, 1579
PspGI	CCWGG	5 cut(s)	354, 381, 1360, 1384, 1579
PspN4I	GGNNCC	7 cut(s)	231, 345, 353, 459, 642, 1120, 1712
PspPI	GGNCC	4 cut(s)	103, 114, 379, 614
PspPPI	RGGWCCY	1 cut(s)	379
PspXI	VCTCGAGB	1 cut(s)	497
PstNI	CAGNNNCTG	1 cut(s)	1475
PsuI	RGATCY	3 cut(s)	229, 1118, 1496
PsyI	GACNNNGTC	1 cut(s)	527
PvuI	CGATCG	3 cut(s)	124, 1436, 1708
PvuII	CAGCTG	1 cut(s)	1662
RsaI	GTAC	8 cut(s)	254, 269, 471, 772, 804, 1314, 1344, 1647
RsaNI	GTAC	8 cut(s)	253, 268, 470, 771, 803, 1313, 1343, 1646
SacI	GAGCTC	1 cut(s)	1780
SacII	CCGCGG	2 cut(s)	50, 236
SaqAI	TTAA	9 cut(s)	201, 366, 510, 543, 660, 1002, 1059, 1563, 1650
SatI	GCNGC	9 cut(s)	50, 63, 360, 548, 981, 1074, 1181, 1605, 1764
Sau96I	GGNCC	4 cut(s)	103, 114, 379, 614
ScaI	AGTACT	1 cut(s)	1344
SchI	GAGTC	4 cut(s)	31, 77, 313, 1469
ScrFI	CCNGG	9 cut(s)	106, 117, 349, 356, 383, 1362, 1386, 1581, 1594
SduI	GDGCHC	4 cut(s)	348, 1274, 1510, 1780
SexAI	ACCWGGT	2 cut(s)	1384, 1579
SfcI	CTRYAG	2 cut(s)	1421, 1500
SfiI	GGCCNNNNNGGCC	1 cut(s)	170
SfoI	GGCGCC	2 cut(s)	459, 642
Sfr274I	CTCGAG	1 cut(s)	497
Sfr303I	CCGCGG	2 cut(s)	50, 236
SgrBI	CCGCGG	2 cut(s)	50, 236
SinI	GGWCC	2 cut(s)	379, 614
SlaI	CTCGAG	1 cut(s)	497
SmlI	CTYRAG	4 cut(s)	497, 509, 659, 861
SmoI	CTYRAG	4 cut(s)	497, 509, 659, 861
SnaBI	TACGTA	1 cut(s)	77
SphI	GCATGC	1 cut(s)	1286
Sse9I	AATT	9 cut(s)	9, 696, 747, 832, 868, 894, 993, 1211, 1318
SseBI	AGGCCT	1 cut(s)	812
SspDI	GGCGCC	2 cut(s)	457, 640
SspMI	CTAG	7 cut(s)	111, 284, 783, 891, 912, 1494, 1691
SstI	GAGCTC	1 cut(s)	1780
StuI	AGGCCT	1 cut(s)	812
StyD4I	CCNGG	9 cut(s)	104, 115, 347, 354, 381, 1360, 1384, 1579, 1592
StyI	CCWWGG	4 cut(s)	110, 174, 372, 1122
TaaI	ACNGT	4 cut(s)	621, 1054, 1252, 1471
TaiI	ACGT	3 cut(s)	79, 258, 528
TaqI	TCGA	4 cut(s)	498, 798, 1338, 1463
TaqII	GACCGA	1 cut(s)	1470
TasI	AATT	9 cut(s)	9, 696, 747, 832, 868, 894, 993, 1211, 1318
TatI	WGTACW	2 cut(s)	802, 1342
TauI	GCSGC	6 cut(s)	52, 65, 362, 550, 1076, 1766
TfiI	GAWTC	6 cut(s)	403, 594, 669, 915, 1196, 1237
Tru1I	TTAA	9 cut(s)	201, 366, 510, 543, 660, 1002, 1059, 1563, 1650
Tru9I	TTAA	9 cut(s)	201, 366, 510, 543, 660, 1002, 1059, 1563, 1650
TscAI	CASTG	3 cut(s)	892, 1057, 1257
TseFI	GTSAC	3 cut(s)	1327, 1390, 1400
TseI	GCWGC	3 cut(s)	980, 1180, 1604
Tsp45I	GTSAC	3 cut(s)	1327, 1390, 1400
TspDTI	ATGAA	4 cut(s)	661, 861, 1324, 1710
TspGWI	ACGGA	4 cut(s)	335, 1289, 1633, 1825
TspRI	CASTG	3 cut(s)	892, 1057, 1257
Tth111I	GACNNNGTC	1 cut(s)	527
Vha464I	CTTAAG	2 cut(s)	509, 659
VpaK11BI	GGWCC	2 cut(s)	379, 614
VspI	ATTAAT	1 cut(s)	1563
XapI	RAATTY	3 cut(s)	832, 868, 1211
XceI	RCATGY	2 cut(s)	1286, 1398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	379
XhoI	CTCGAG	1 cut(s)	497
XmaJI	CCTAGG	1 cut(s)	110
XspI	CTAG	7 cut(s)	111, 284, 783, 891, 912, 1494, 1691
ZraI	GACGTC	1 cut(s)	526
ZrmI	AGTACT	1 cut(s)	1344
Zsp2I	ATGCAT	1 cut(s)	1288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.