Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002451

Physical Location & Seq

Genomic Coordinates Reverse (-)

25701874 .. 25709019

Gene Structure 7146 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1198900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGTTGAGAAGACCAAGGAACCTAAGAAGGGCTCTATCACCGAAGAAGATACCGCCACTCTCCTGCAAAGGTACGCGCCGACGACTGTACTGGCCCTGCTGCAAGAAGTGGCGAACTCGCCAAAGGGGAAGATTGATTGGAATCGGTTGGTGGCCAAGACTTCAACTGGAATTTCCAATGCCAGAGAGTATCAGATGCTTTGGCGCCATTTAGCGTATCGTGAAGCACTGCTTGACAAATTAGACAATGGGGCTCAACCTTTGGATGATGATAGTGATTTAGAATATGAAGTGGAAGCTTATCCTGCTGTTAGTGCTGAAGCTTCAACAGAAGCTGTGGCATGTGTGAAGGTTCTAATTTCATCTGGCTTATCAAGTGACTCAAGTCATCAAAATGGTTTAACTGTTGAGGGTCTGTTGTCCATGAATTTACCCAATCAGTCATCTAGATCGCAACAAAATTTACAACCAACATGTTCAATGCAAGGGAAGAACATTACAGTTCCGGTTTCTGTTCTGAAAAATTCCCTCCCTGCAGTGACAACAACTACAGTGGAAACTACTGAAGGAGATGATGCATTAGGAAATGGCATGGCTCCTCGAAAGAGAAGAAAGAACTGGTCTGAGGCTGAGGATTTGGAGCTGATTGCTGCTGTGAAGAAATATGGTGAAGGAAATTGGGCAAATATGTTACGAGGAGATTTCAAAGGGGACAGAACACCTAGTCAGCTAGCTCAGAGGTGGAAAAAGATTAGGGGGAACAATGACTTGAATGTTGGAGGCAGAAGTAAACTTTCAGAAGCACAGTTGGCAGCTCGTCATTCATTGTCCATAGCCCTGAATATGCCTAACATAACAGCTAATACAATTGGCACGGCTGGGACAAGTACACACAGCATGCTTGCTGGAACAAATGCAACTAGTAACTCTCTGCATTTTACTACTGCTGAAGCTAAACCGCTGTCCCAAAAAGGCCTTGACCCAGTGAAAGCTGAAGCAAAACCGCAGTCCCAGGAAGGCCTTAACCCAGCAAAAACATATCAAATGGGATTGTCAGGTTCCACGACAAAAGCTCCAGTGATCGCCAAAAAAACTATATCGAAGCTTACTGATAGTAAGGTAAGAGCTACTGCGGTTGCTGCTGGGGCACGCATTGCTTTTCCTTCAGATGCTGCATCACTTCTCAAGGCTGCTCAGGCAAAGAATGCTGTCCATATCATGCCTACAGTTTGTTCTTCTACCCAATCGGTTAAACCTGGTGGCATGTCTACCCACTCAGAACTTCCTCCTAATGTACATATACTGCGTAGCAGCTCGGTTGCTACGCCACTTTCGACCCCTTCATCCACTGCTGCCACACCTAGCACCACACGCCCTCGGTCTTCAAAAGTTCTTCCTCAGGCAAGTCAGCACACTCCAACAAAAGACGCAATGTTATCCAGACAGACCAATGGCGTTAGCTGTAACCTGGATGCTGAGCAGGTCCAAGATGGAGCAGTTATCTTTAGGGATGGACCGAATGACCAAGTTCAAAAGGATAAAAGGGATTCACCTGACCAGAAAGCTGAGTCAAAAAATTTATCAACTAATGCTGAAAAACCCGTTGATACTCCGAAGATTGAAAGTGATGAGACCGGTAGTAAAGCTGTCACGGGTGTACAAGCCAAAGAGGGAAAAAGTGCAAAAGATAATGAGATTCCGCGTTCATCTGTTAATGTAGGAGCCAGTGACCCATCTGCAGTTGACGGTTGTGAAAACCGAAGCGCAAGTGAGAAGCAGACAGATCTTGCCATTGAAACAGATGGATGCAAGGGAAAACAGGTCGTACGCAAAGGGGAGGGTGACATTAACATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

619

Amino Acids

65.63

Weight (kDa)

8.63

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	204 - 251	1.2e-08	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	207 - 251	2.8e-06	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002451)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09710 AT1G09710 AT1G09710 AT1G09710 AT1G09710 AT1G58220 AT1G58220
fragaria_vesca	FvH4_4g17970 FvH4_5g34820 FvH4_5g34820 FvH4_5g34820 FvH4_5g34820 FvH4_5g34820 FvH4_5g34820
malus_domestica	MD08G1198900.v1.1 MD15G1385800.v1.1 MD15G1386000.v1.1
prunus_persica	Prupe.1G532600_v2.0.a1 Prupe.1G532600_v2.0.a1 Prupe.1G532600_v2.0.a1 Prupe.1G532600_v2.0.a1 Prupe.1G532700_v2.0.a1 Prupe.1G532700_v2.0.a1 Prupe.1G532700_v2.0.a1 Prupe.1G532700_v2.0.a1
pyrus_communis	pycom08g17100 pycom08g17280 pycom15g34620
rosa_chinensis	RchiOBHm_Chr7g0235501
rosa_laevigata	RLG00000001154
rosa_multiflora	Rmu_sc0002892.1_g000016 Rmu_sc0004498.1_g000008 Rmu_sc0039003.1_g000001
rosa_roxburghii	Rroxscaffold_3G00225950
rosa_rugosa	Rorug07G0291300 Rorug07G0291400
rosa_samantha	Rh7AG446400 Rh7BG418100 Rh7CG466300 Rh7DG434900
rosa_wichuraiana	Rw7G037060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1471
AccB1I	GGYRCC	1 cut(s)	204
AccI	GTMKAC	1 cut(s)	1268
AccII	CGCG	2 cut(s)	77, 1702
AciI	CCGC	5 cut(s)	54, 959, 1004, 1133, 1700
AcoI	YGGCCR	1 cut(s)	153
AcsI	RAATTY	5 cut(s)	171, 427, 460, 523, 1576
AcuI	CTGAAG	5 cut(s)	339, 585, 969, 1014, 1149
AcyI	GRCGYC	1 cut(s)	205
AfaI	GTAC	6 cut(s)	74, 90, 889, 1296, 1659, 1827
AfiI	CCNNNNNNNGG	1 cut(s)	29
AflIII	ACRYGT	1 cut(s)	473
AgeI	ACCGGT	1 cut(s)	1634
AgsI	TTSAA	9 cut(s)	165, 327, 480, 706, 772, 1386, 1532, 1622, 1796
AhlI	ACTAGT	1 cut(s)	920
AjnI	CCWGG	3 cut(s)	1011, 1255, 1467
Alw26I	GTCTC	1 cut(s)	1625
AlwNI	CAGNNNCTG	2 cut(s)	335, 1172
AoxI	GGCC	4 cut(s)	93, 153, 973, 1018
ApeKI	GCWGC	8 cut(s)	100, 650, 812, 1139, 1172, 1190, 1311, 1352
ApoI	RAATTY	5 cut(s)	171, 427, 460, 523, 1576
ArsI	GACNNNNNNTTYG	2 cut(s)	992, 1024
AsiGI	ACCGGT	1 cut(s)	1634
AspLEI	GCGC	3 cut(s)	79, 207, 1766
AspS9I	GGNCC	3 cut(s)	94, 1483, 1514
AsuHPI	GGTGA	4 cut(s)	31, 680, 1542, 1853
AsuNHI	GCTAGC	1 cut(s)	730
AvaII	GGWCC	2 cut(s)	1483, 1514
AxyI	CCTNAGG	1 cut(s)	1398
BaeGI	GKGCMC	1 cut(s)	1150
BalI	TGGCCA	1 cut(s)	155
BanI	GGYRCC	1 cut(s)	204
BanII	GRGCYC	2 cut(s)	35, 256
BbsI	GAAGAC	2 cut(s)	17, 1374
BbvCI	CCTCAGC	1 cut(s)	630
BbvI	GCAGC	8 cut(s)	87, 637, 824, 1126, 1159, 1177, 1323, 1339
BccI	CCATC	4 cut(s)	1484, 1505, 1741, 1796
BceAI	ACGGC	1 cut(s)	891
BciT130I	CCWGG	3 cut(s)	1013, 1257, 1469
BcoDI	GTCTC	1 cut(s)	1625
BcuI	ACTAGT	1 cut(s)	920
BfaI	CTAG	6 cut(s)	447, 723, 731, 921, 1362, 1855
BfmI	CTRYAG	4 cut(s)	534, 549, 1224, 1737
BfoI	RGCGCY	1 cut(s)	208
BfuAI	ACCTGC	1 cut(s)	1471
BglII	AGATCT	1 cut(s)	1783
BisI	GCNGC	8 cut(s)	101, 651, 813, 1140, 1173, 1191, 1312, 1353
BlpI	GCTNAGC	1 cut(s)	1476
BlsI	GCNGC	8 cut(s)	102, 652, 814, 1141, 1174, 1192, 1313, 1354
Bme1390I	CCNGG	3 cut(s)	1013, 1257, 1469
Bme18I	GGWCC	2 cut(s)	1483, 1514
BmgT120I	GGNCC	3 cut(s)	94, 1483, 1514
BmiI	GGNNCC	5 cut(s)	21, 206, 597, 1060, 1723
BmrFI	CCNGG	3 cut(s)	1013, 1257, 1469
BmrI	ACTGGG	1 cut(s)	977
BmsI	GCATC	6 cut(s)	186, 565, 1159, 1184, 1462, 1796
BmtI	GCTAGC	1 cut(s)	734
BmuI	ACTGGG	1 cut(s)	977
BoxI	GACNNNNGTC	1 cut(s)	384
BpiI	GAAGAC	2 cut(s)	17, 1374
BpmI	CTGGAG	1 cut(s)	1059
Bpu10I	CCTNAGC	2 cut(s)	630, 1194
Bpu1102I	GCTNAGC	1 cut(s)	1476
BpuEI	CTTGAG	2 cut(s)	367, 1169
BsaBI	GATNNNNATC	1 cut(s)	141
BsaHI	GRCGYC	1 cut(s)	205
BsaI	GGTCTC	1 cut(s)	1625
BsaJI	CCNNGG	3 cut(s)	15, 1011, 1376
BsaWI	WCCGGW	2 cut(s)	505, 1634
BsaXI	ACNNNNNCTCC	2 cut(s)	1057, 1087
Bsc4I	CCNNNNNNNGG	1 cut(s)	29
Bse118I	RCCGGY	1 cut(s)	1634
Bse1I	ACTGG	6 cut(s)	96, 172, 623, 983, 1076, 1725
Bse21I	CCTNAGG	1 cut(s)	1398
Bse3DI	GCAATG	2 cut(s)	1152, 1437
Bse8I	GATNNNNATC	1 cut(s)	141
BseBI	CCWGG	3 cut(s)	1013, 1257, 1469
BseDI	CCNNGG	3 cut(s)	15, 1011, 1376
BseGI	GGATG	5 cut(s)	271, 1343, 1477, 1516, 1811
BseJI	GATNNNNATC	1 cut(s)	141
BseLI	CCNNNNNNNGG	1 cut(s)	29
BseMI	GCAATG	2 cut(s)	1152, 1437
BseMII	CTCAG	8 cut(s)	615, 621, 749, 1208, 1290, 1412, 1467, 1557
BseNI	ACTGG	6 cut(s)	96, 172, 623, 983, 1076, 1725
BseRI	GAGGAG	2 cut(s)	588, 711
BseSI	GKGCMC	1 cut(s)	1150
BseXI	GCAGC	8 cut(s)	87, 637, 824, 1126, 1159, 1177, 1323, 1339
BseYI	CCCAGC	3 cut(s)	878, 1027, 1142
Bsh1236I	CGCG	2 cut(s)	77, 1702
BshFI	GGCC	4 cut(s)	95, 155, 975, 1020
BshNI	GGYRCC	1 cut(s)	204
BshTI	ACCGGT	1 cut(s)	1634
BsiSI	CCGG	2 cut(s)	506, 1635
BsiWI	CGTACG	1 cut(s)	1825
BslFI	GGGAC	4 cut(s)	725, 895, 949, 994
BslI	CCNNNNNNNGG	1 cut(s)	29
BsmAI	GTCTC	1 cut(s)	1625
BsmFI	GGGAC	4 cut(s)	725, 895, 949, 994
BsmI	GAATGC	1 cut(s)	1210
BsnI	GGCC	4 cut(s)	95, 155, 975, 1020
Bso31I	GGTCTC	1 cut(s)	1625
Bsp1286I	GDGCHC	3 cut(s)	35, 256, 1150
Bsp1407I	TGTACA	2 cut(s)	1294, 1657
Bsp143I	GATC	3 cut(s)	449, 1080, 1783
Bsp1720I	GCTNAGC	1 cut(s)	1476
BspACI	CCGC	5 cut(s)	54, 959, 1004, 1133, 1700
BspANI	GGCC	4 cut(s)	95, 155, 975, 1020
BspCNI	CTCAG	8 cut(s)	616, 622, 748, 1207, 1289, 1411, 1468, 1558
BspFNI	CGCG	2 cut(s)	77, 1702
BspLI	GGNNCC	5 cut(s)	21, 206, 597, 1060, 1723
BspMAI	CTGCAG	2 cut(s)	538, 1741
BspMI	ACCTGC	1 cut(s)	1471
BspOI	GCTAGC	1 cut(s)	734
BspT107I	GGYRCC	1 cut(s)	204
BspTNI	GGTCTC	1 cut(s)	1625
BsrDI	GCAATG	2 cut(s)	1152, 1437
BsrFI	RCCGGY	1 cut(s)	1634
BsrGI	TGTACA	2 cut(s)	1294, 1657
BsrI	ACTGG	6 cut(s)	96, 172, 623, 983, 1076, 1725
BssAI	RCCGGY	1 cut(s)	1634
BssECI	CCNNGG	3 cut(s)	15, 1011, 1376
BssMI	GATC	3 cut(s)	449, 1080, 1783
BssNI	GRCGYC	1 cut(s)	205
BssT1I	CCWWGG	1 cut(s)	15
Bst2UI	CCWGG	3 cut(s)	1013, 1257, 1469
Bst4CI	ACNGT	7 cut(s)	88, 406, 502, 553, 807, 1228, 1748
BstACI	GRCGYC	1 cut(s)	205
BstAPI	GCANNNNNTGC	2 cut(s)	1154, 1205
BstAUI	TGTACA	2 cut(s)	1294, 1657
BstC8I	GCNNGC	4 cut(s)	732, 899, 903, 1150
BstDEI	CTNAG	9 cut(s)	24, 624, 630, 735, 1194, 1276, 1398, 1476, 1566
BstF5I	GGATG	5 cut(s)	271, 1343, 1477, 1516, 1811
BstFNI	CGCG	2 cut(s)	77, 1702
BstH2I	RGCGCY	1 cut(s)	208
BstHHI	GCGC	3 cut(s)	79, 207, 1766
BstKTI	GATC	3 cut(s)	452, 1083, 1786
BstMAI	GTCTC	1 cut(s)	1625
BstMBI	GATC	3 cut(s)	449, 1080, 1783
BstMWI	GCNNNNNNNGC	9 cut(s)	305, 314, 809, 1139, 1145, 1154, 1196, 1205, 1371
BstNI	CCWGG	3 cut(s)	1013, 1257, 1469
BstNSI	RCATGY	4 cut(s)	345, 477, 901, 1267
BstPAI	GACNNNNGTC	1 cut(s)	384
BstSCI	CCNGG	3 cut(s)	1011, 1255, 1467
BstSFI	CTRYAG	4 cut(s)	534, 549, 1224, 1737
BstSLI	GKGCMC	1 cut(s)	1150
BstUI	CGCG	2 cut(s)	77, 1702
BstV1I	GCAGC	8 cut(s)	87, 637, 824, 1126, 1159, 1177, 1323, 1339
BstV2I	GAAGAC	2 cut(s)	17, 1374
BstX2I	RGATCY	1 cut(s)	1783
BstYI	RGATCY	1 cut(s)	1783
Bsu36I	CCTNAGG	1 cut(s)	1398
BsuRI	GGCC	4 cut(s)	95, 155, 975, 1020
BtsCI	GGATG	5 cut(s)	271, 1343, 1477, 1516, 1811
BtsI	GCAGTG	3 cut(s)	227, 543, 1347
BtsIMutI	CAGTG	7 cut(s)	227, 543, 558, 990, 1083, 1347, 1732
BveI	ACCTGC	1 cut(s)	1471
Cac8I	GCNNGC	4 cut(s)	732, 899, 903, 1150
CaiI	CAGNNNCTG	2 cut(s)	335, 1172
CfoI	GCGC	3 cut(s)	79, 207, 1766
Cfr10I	RCCGGY	1 cut(s)	1634
Cfr13I	GGNCC	3 cut(s)	94, 1483, 1514
CseI	GACGC	1 cut(s)	1436
CsiI	ACCWGGT	1 cut(s)	1255
Csp6I	GTAC	6 cut(s)	73, 89, 888, 1295, 1658, 1826
CspAI	ACCGGT	1 cut(s)	1634
CspCI	CAANNNNNGTGG	2 cut(s)	534, 569
CviAII	CATG	7 cut(s)	342, 424, 474, 592, 898, 1219, 1264
CviQI	GTAC	6 cut(s)	73, 89, 888, 1295, 1658, 1826
DdeI	CTNAG	9 cut(s)	24, 624, 630, 735, 1194, 1276, 1398, 1476, 1566
DinI	GGCGCC	1 cut(s)	206
DpnI	GATC	3 cut(s)	451, 1082, 1785
DpnII	GATC	3 cut(s)	449, 1080, 1783
EaeI	YGGCCR	1 cut(s)	153
Eco130I	CCWWGG	1 cut(s)	15
Eco147I	AGGCCT	2 cut(s)	975, 1020
Eco24I	GRGCYC	2 cut(s)	35, 256
Eco31I	GGTCTC	1 cut(s)	1625
Eco47I	GGWCC	2 cut(s)	1483, 1514
Eco57I	CTGAAG	5 cut(s)	339, 585, 969, 1014, 1149
Eco81I	CCTNAGG	1 cut(s)	1398
EcoRII	CCWGG	3 cut(s)	1011, 1255, 1467
EcoT14I	CCWWGG	1 cut(s)	15
EcoT22I	ATGCAT	1 cut(s)	580
EcoT38I	GRGCYC	2 cut(s)	35, 256
EgeI	GGCGCC	1 cut(s)	206
EheI	GGCGCC	1 cut(s)	206
ErhI	CCWWGG	1 cut(s)	15
FaeI	CATG	7 cut(s)	345, 427, 477, 595, 901, 1222, 1267
FalI	AAGNNNNNCTT	2 cut(s)	216, 248
FaqI	GGGAC	4 cut(s)	725, 895, 949, 994
FatI	CATG	7 cut(s)	341, 423, 473, 591, 897, 1218, 1263
FblI	GTMKAC	1 cut(s)	1268
Fnu4HI	GCNGC	8 cut(s)	101, 651, 813, 1140, 1173, 1191, 1312, 1353
FokI	GGATG	5 cut(s)	278, 1330, 1484, 1523, 1818
FriOI	GRGCYC	2 cut(s)	35, 256
Fsp4HI	GCNGC	8 cut(s)	101, 651, 813, 1140, 1173, 1191, 1312, 1353
FspBI	CTAG	6 cut(s)	447, 723, 731, 921, 1362, 1855
GlaI	GCGC	3 cut(s)	78, 206, 1765
GluI	GCNGC	8 cut(s)	101, 651, 813, 1140, 1173, 1191, 1312, 1353
GsaI	CCCAGC	3 cut(s)	882, 1031, 1146
GsuI	CTGGAG	1 cut(s)	1059
HaeII	RGCGCY	1 cut(s)	208
HaeIII	GGCC	4 cut(s)	95, 155, 975, 1020
HapII	CCGG	2 cut(s)	506, 1635
HgaI	GACGC	1 cut(s)	1436
HhaI	GCGC	3 cut(s)	79, 207, 1766
Hin1I	GRCGYC	1 cut(s)	205
Hin1II	CATG	7 cut(s)	345, 427, 477, 595, 901, 1222, 1267
Hin6I	GCGC	3 cut(s)	77, 205, 1764
HinP1I	GCGC	3 cut(s)	77, 205, 1764
HincII	GTYRAC	1 cut(s)	1744
HindII	GTYRAC	1 cut(s)	1744
HindIII	AAGCTT	3 cut(s)	297, 321, 1103
HinfI	GANTC	5 cut(s)	142, 380, 1547, 1568, 1696
HpaII	CCGG	2 cut(s)	506, 1635
HphI	GGTGA	4 cut(s)	31, 680, 1542, 1853
Hpy166II	GTNNAC	5 cut(s)	791, 890, 1269, 1658, 1744
Hpy188I	TCNGA	8 cut(s)	195, 519, 625, 738, 799, 1168, 1279, 1614
Hpy188III	TCNNGA	3 cut(s)	221, 447, 1440
Hpy8I	GTNNAC	5 cut(s)	791, 890, 1269, 1658, 1744
Hpy99I	CGWCG	1 cut(s)	85
HpyAV	CCTTC	7 cut(s)	22, 343, 560, 665, 1010, 1173, 1350
HpyCH4III	ACNGT	7 cut(s)	88, 406, 502, 553, 807, 1228, 1748
HpyF10VI	GCNNNNNNNGC	9 cut(s)	305, 314, 809, 1139, 1145, 1154, 1196, 1205, 1371
HpyF3I	CTNAG	9 cut(s)	24, 624, 630, 735, 1194, 1276, 1398, 1476, 1566
Hsp92I	GRCGYC	1 cut(s)	205
Hsp92II	CATG	7 cut(s)	345, 427, 477, 595, 901, 1222, 1267
HspAI	GCGC	3 cut(s)	77, 205, 1764
KasI	GGCGCC	1 cut(s)	204
Kzo9I	GATC	3 cut(s)	449, 1080, 1783
LmnI	GCTCC	5 cut(s)	601, 640, 1078, 1493, 1721
Lsp1109I	GCAGC	8 cut(s)	87, 637, 824, 1126, 1159, 1177, 1323, 1339
LweI	GCATC	6 cut(s)	186, 565, 1159, 1184, 1462, 1796
MabI	ACCWGGT	1 cut(s)	1255
MaeI	CTAG	6 cut(s)	447, 723, 731, 921, 1362, 1855
MaeIII	GTNAC	8 cut(s)	377, 538, 690, 923, 1463, 1648, 1727, 1841
MalI	GATC	3 cut(s)	451, 1082, 1785
MboI	GATC	3 cut(s)	449, 1080, 1783
MfeI	CAATTG	1 cut(s)	867
MflI	RGATCY	1 cut(s)	1783
MhlI	GDGCHC	3 cut(s)	35, 256, 1150
MlsI	TGGCCA	1 cut(s)	155
MluCI	AATT	9 cut(s)	171, 239, 357, 427, 460, 523, 676, 867, 1576
MluNI	TGGCCA	1 cut(s)	155
Mly113I	GGCGCC	1 cut(s)	205
MlyI	GAGTC	2 cut(s)	374, 1577
MmeI	TCCRAC	2 cut(s)	757, 1442
Mox20I	TGGCCA	1 cut(s)	155
Mph1103I	ATGCAT	1 cut(s)	580
MscI	TGGCCA	1 cut(s)	155
MseI	TTAA	5 cut(s)	401, 1023, 1251, 1713, 1848
MslI	CAYNNNNRTG	1 cut(s)	393
Msp20I	TGGCCA	1 cut(s)	155
MspA1I	CMGCKG	1 cut(s)	961
MspI	CCGG	2 cut(s)	506, 1635
MspR9I	CCNGG	3 cut(s)	1013, 1257, 1469
MunI	CAATTG	1 cut(s)	867
Mva1269I	GAATGC	1 cut(s)	1210
MvaI	CCWGG	3 cut(s)	1013, 1257, 1469
MvnI	CGCG	2 cut(s)	77, 1702
MwoI	GCNNNNNNNGC	9 cut(s)	305, 314, 809, 1139, 1145, 1154, 1196, 1205, 1371
NarI	GGCGCC	1 cut(s)	205
NdeII	GATC	3 cut(s)	449, 1080, 1783
NheI	GCTAGC	1 cut(s)	730
NlaIII	CATG	7 cut(s)	345, 427, 477, 595, 901, 1222, 1267
NlaIV	GGNNCC	5 cut(s)	21, 206, 597, 1060, 1723
NmuCI	GTSAC	5 cut(s)	377, 538, 1648, 1727, 1841
NsiI	ATGCAT	1 cut(s)	580
NspI	RCATGY	4 cut(s)	345, 477, 901, 1267
PaeI	GCATGC	1 cut(s)	901
PceI	AGGCCT	2 cut(s)	975, 1020
PciI	ACATGT	1 cut(s)	473
PcsI	WCGNNNNNNNCGW	1 cut(s)	1331
PctI	GAATGC	1 cut(s)	1210
PfeI	GAWTC	3 cut(s)	142, 1547, 1696
Pfl23II	CGTACG	1 cut(s)	1825
PinAI	ACCGGT	1 cut(s)	1634
PkrI	GCNGC	8 cut(s)	102, 652, 814, 1141, 1174, 1192, 1313, 1354
PleI	GAGTC	2 cut(s)	374, 1576
PluTI	GGCGCC	1 cut(s)	208
PpsI	GAGTC	2 cut(s)	374, 1576
PscI	ACATGT	1 cut(s)	473
PshAI	GACNNNNGTC	1 cut(s)	384
Psp6I	CCWGG	3 cut(s)	1011, 1255, 1467
PspFI	CCCAGC	3 cut(s)	878, 1027, 1142
PspGI	CCWGG	3 cut(s)	1011, 1255, 1467
PspLI	CGTACG	1 cut(s)	1825
PspN4I	GGNNCC	5 cut(s)	21, 206, 597, 1060, 1723
PspPI	GGNCC	3 cut(s)	94, 1483, 1514
PstI	CTGCAG	2 cut(s)	538, 1741
PstNI	CAGNNNCTG	2 cut(s)	335, 1172
PsuI	RGATCY	1 cut(s)	1783
RsaI	GTAC	6 cut(s)	74, 90, 889, 1296, 1659, 1827
RsaNI	GTAC	6 cut(s)	73, 89, 888, 1295, 1658, 1826
RseI	CAYNNNNRTG	1 cut(s)	393
SaqAI	TTAA	5 cut(s)	401, 1023, 1251, 1713, 1848
SatI	GCNGC	8 cut(s)	101, 651, 813, 1140, 1173, 1191, 1312, 1353
Sau3AI	GATC	3 cut(s)	449, 1080, 1783
Sau96I	GGNCC	3 cut(s)	94, 1483, 1514
SchI	GAGTC	2 cut(s)	374, 1577
ScrFI	CCNGG	3 cut(s)	1013, 1257, 1469
SduI	GDGCHC	3 cut(s)	35, 256, 1150
SexAI	ACCWGGT	1 cut(s)	1255
SfaNI	GCATC	6 cut(s)	186, 565, 1159, 1184, 1462, 1796
SfcI	CTRYAG	4 cut(s)	534, 549, 1224, 1737
SfoI	GGCGCC	1 cut(s)	206
SinI	GGWCC	2 cut(s)	1483, 1514
SmiMI	CAYNNNNRTG	1 cut(s)	393
SmlI	CTYRAG	2 cut(s)	382, 1184
SmoI	CTYRAG	2 cut(s)	382, 1184
SpeI	ACTAGT	1 cut(s)	920
SphI	GCATGC	1 cut(s)	901
Sse9I	AATT	9 cut(s)	171, 239, 357, 427, 460, 523, 676, 867, 1576
SseBI	AGGCCT	2 cut(s)	975, 1020
SsiI	CCGC	5 cut(s)	54, 959, 1004, 1133, 1700
SspDI	GGCGCC	1 cut(s)	204
SspMI	CTAG	6 cut(s)	447, 723, 731, 921, 1362, 1855
StuI	AGGCCT	2 cut(s)	975, 1020
StyD4I	CCNGG	3 cut(s)	1011, 1255, 1467
StyI	CCWWGG	1 cut(s)	15
TaaI	ACNGT	7 cut(s)	88, 406, 502, 553, 807, 1228, 1748
TaqI	TCGA	3 cut(s)	601, 1100, 1334
TaqII	GACCGA	2 cut(s)	1368, 1531
TasI	AATT	9 cut(s)	171, 239, 357, 427, 460, 523, 676, 867, 1576
TatI	WGTACW	4 cut(s)	88, 887, 1294, 1657
TfiI	GAWTC	3 cut(s)	142, 1547, 1696
Tru1I	TTAA	5 cut(s)	401, 1023, 1251, 1713, 1848
Tru9I	TTAA	5 cut(s)	401, 1023, 1251, 1713, 1848
TscAI	CASTG	7 cut(s)	234, 543, 558, 990, 1083, 1354, 1732
TseFI	GTSAC	5 cut(s)	377, 538, 1648, 1727, 1841
TseI	GCWGC	8 cut(s)	100, 650, 812, 1139, 1172, 1190, 1311, 1352
Tsp45I	GTSAC	5 cut(s)	377, 538, 1648, 1727, 1841
TspDTI	ATGAA	6 cut(s)	303, 351, 440, 813, 1332, 1695
TspRI	CASTG	7 cut(s)	234, 543, 558, 990, 1083, 1354, 1732
VpaK11BI	GGWCC	2 cut(s)	1483, 1514
XapI	RAATTY	5 cut(s)	171, 427, 460, 523, 1576
XbaI	TCTAGA	1 cut(s)	446
XceI	RCATGY	4 cut(s)	345, 477, 901, 1267
XmiI	GTMKAC	1 cut(s)	1268
XspI	CTAG	6 cut(s)	447, 723, 731, 921, 1362, 1855
Zsp2I	ATGCAT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.