Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002285

Physical Location & Seq

Genomic Coordinates Reverse (-)

26541730 .. 26547707

Gene Structure 5978 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1205000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGTCATCTCAAGGGGTTCGACTGATTATTCAGGACCGTTATGTGGTCATGGATAATGGCATACTTCAAGTTACGTTATCAAATCCAGATGGAATTGTTACCAGAATACAATACAGTGGCATCGACAATTTGCTCGAAGTTCTAAATGAAGAAGTAAATAGAGGGTATTGGGACCTTGTTTGGAGTGAAGCAGGAAGTGTGGGCACAACCGGGACATTTGATGTGATTAAAGGGACAAAATTCAAGGTTAGAGTGGAAAACGAGGAACAAGTAGAGATCTCCTTCACAAGAAAGTGGAATCCGTCTCAAGAGGGAAAACTTGTCCCTCTCAATATAGATAAAAGATTTATAATGCTTCGTAATTCTTCAGGCTTCTACTCATATGCCATCTATGAGCACTTGAAAGAATGGCCTCCTTTCAACCTTCCACAAACCAGGATTACCTTCAAGCTCAGAAAAGAAAAGTTTCACTATATGGCCATAGCAGATAACAGGCAAAGATACATGCCCCTTCCGGACGATCGATCACCTGAGAGAAGTCAAGCCCTAGCTTACCCTGAAGCAGTCCTTCTTGTCGATCCTATAGAGCCAGAATTCAAGGGAGAGGTGGATGATAAGTATGAATACTCCTGCGAGAATCAAAACCTGCATGTTCATGGTTGGGTATGTATGGACCCACCCATTGGCTTTTGGCAAATAACGCCCAGTGATGAATTTCGATCTGGTGGACCTCTCAAACAGAATCTCACCTCCCATGTTGGCCCCACTTGCCTTGCGATGTTTCTTAGCGCTCATTATTCAGGAGAAGACCTAGTGCTGAAGCTGAAACCAGATGAGCCATGGAAGAAAATTTTTGGTCCTGTATTCATTTATCTTAATTCTGTGTCCGATGCAACTGATCCATCACCACTTTGGGATGATGCAAAAAAACAGATGATGACAGAAGTGCAAAGCTGGCCTTACAAGTTTCCTGCCTCCGAGGATTATCCGCCGTCAGATCAACGGGGCAATGTTAAGGGTAGAATACAGATTCTGGATAGGTATGTTAGTAAAGATCACGTACCTGGAGACGGTGCGTATGTAGGCTTGGCACCACCAGGAGACGTTGGATCATGGCAAAGAGATTGTAAGGGCTACCAGTTCTGGACAAGGGCAGATGAGAAAGGATATTACTCCATACAGAATATACGCGAGGGTGACTATAATCTCTATGCATGGTGTCCTGGTTTTATTGGAGATTATCGATATGACGCAACTGTTAGCATAACCGCAGGTTGTGATATTGATGTGGATGATATTGTATATGAGCCTCCAAGAGATGGACCAACGTTGTGGGAAATTGGCATCCCTGATCGTTCTGCTGCTGAATTCTATGTTCCTGATCCTAATCCAATGTATATCAATAAACTTTATGTCAACCATCCTGATAGGTTTAGGCAGTACGGATTATGGGAAAGATATCAAGAGTTATATCCTGATGAAGATTTAATTTACAGCATCGGCACCAGTGACTATACTAAAGACTGGTTCTTCGCCCAGGTTCCTAGGAAGAAAGATGAAGACACATATCAAGGCACTACATGGCAAATCAAGTTTCAACTTAAGCATTTCAGTGAAAGTGATACCCTCAAACTTCGAATAGCCCTTGCCACTGCAAACATTGCAGAATTACAGATTCGAATAAATGATTCGAAAGCCGACCCTCCGCTGTTTACGACCGGAGTGATTGGAAACGACAATACAATTGCCAGGCATGGAATCCATGGTCTGTACCGGCTGTATAACATAGATATACCAGGCACATTGCTGGTGGAAGGAGCCAATACCCTATTTCTAACGCAACCAATGAGCAGTAGCCCTTTGGCACCTTTCCACGGACTCATGTATGACTATATTCGTTTAGAAGGTCCATCATCTTCTTCTTCTTCCAGTACCACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

647

Amino Acids

73.95

Weight (kDa)

5.01

Isoelectric Point (pI)

39.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhamnogal_lyase	PF06045	4 - 203	8.7e-98	Rhamnogalacturonate lyase family
fn3_3	PF14686	357 - 429	1.8e-27	Polysaccharide lyase family 4, domain II
CBM-like	PF14683	443 - 634	1.8e-50	Polysaccharide lyase family 4, domain III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24430 AT4G24430
fragaria_vesca	FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34150
malus_domestica	MD08G1204400.v1.1 MD08G1205000.v1.1
prunus_persica	Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536700_v2.0.a1 Prupe.1G536700_v2.0.a1
pyrus_communis	pycom08g17670 pycom08g17720
rosa_chinensis	RchiOBHm_Chr7g0233711 RchiOBHm_Chr7g0233721 RchiOBHm_Chr7g0233731 RchiOBHm_Chr7g0233741
rosa_laevigata	RLG00000001237 RLG00000001239 RLG00000001240
rosa_multiflora	Rmu_sc0000740.1_g000047 Rmu_sc0000740.1_g000058 Rmu_sc0010145.1_g000001 Rmu_sc0011583.1_g000004
rosa_roxburghii	Rroxscaffold_3G00227030 Rroxscaffold_3G00227050 Rroxscaffold_3G00227070
rosa_rugosa	Rorug07G0282300 Rorug07G0282300
rosa_samantha	Rh7AG438600 Rh7AG438700 Rh7BG411300 Rh7CG458200 Rh7CG458300 Rh7CG458400 Rh7DG428200
rosa_wichuraiana	Rw7G036170 Rw7G036180 Rw7G036230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	350
Acc36I	ACCTGC	2 cut(s)	654, 1262
AccB1I	GGYRCC	3 cut(s)	1090, 1502, 1864
AccB7I	CCANNNNNTGG	2 cut(s)	683, 1319
AccII	CGCG	1 cut(s)	1191
AccIII	TCCGGA	1 cut(s)	514
AciI	CCGC	3 cut(s)	989, 1269, 1706
AclI	AACGTT	1 cut(s)	1328
AclWI	GGATC	4 cut(s)	572, 893, 1117, 1376
AcoI	YGGCCR	1 cut(s)	477
AcsI	RAATTY	5 cut(s)	239, 593, 713, 849, 1367
AcuI	CTGAAG	3 cut(s)	351, 579, 839
AfaI	GTAC	4 cut(s)	1062, 1442, 1772, 1933
AfeI	AGCGCT	1 cut(s)	790
AfiI	CCNNNNNNNGG	5 cut(s)	43, 683, 1070, 1319, 1874
AflII	CTTAAG	1 cut(s)	1601
AgsI	TTSAA	7 cut(s)	68, 244, 403, 421, 448, 598, 1598
AjnI	CCWGG	7 cut(s)	434, 1063, 1096, 1222, 1536, 1748, 1795
AluBI	AGCT	4 cut(s)	451, 551, 823, 954
AluI	AGCT	4 cut(s)	451, 551, 823, 954
Alw21I	GWGCWC	1 cut(s)	399
Alw26I	GTCTC	3 cut(s)	309, 1062, 1095
AlwI	GGATC	4 cut(s)	572, 893, 1117, 1376
AlwNI	CAGNNNCTG	1 cut(s)	1033
Aor13HI	TCCGGA	1 cut(s)	514
Aor51HI	AGCGCT	1 cut(s)	790
AoxI	GGCC	4 cut(s)	410, 477, 760, 956
ApeKI	GCWGC	1 cut(s)	1361
ApoI	RAATTY	5 cut(s)	239, 593, 713, 849, 1367
ArsI	GACNNNNNNTTYG	2 cut(s)	1514, 1546
Asp700I	GAANNNNTTC	1 cut(s)	465
AspA2I	CCTAGG	1 cut(s)	1544
AspLEI	GCGC	1 cut(s)	791
AspS9I	GGNCC	8 cut(s)	34, 172, 673, 728, 761, 857, 1322, 1907
AsuC2I	CCSGG	1 cut(s)	211
AsuHPI	GGTGA	4 cut(s)	519, 739, 897, 1208
AsuII	TTCGAA	3 cut(s)	1636, 1678, 1691
AvaII	GGWCC	7 cut(s)	34, 172, 673, 728, 857, 1322, 1907
AvrII	CCTAGG	1 cut(s)	1544
BaeGI	GKGCMC	1 cut(s)	206
BalI	TGGCCA	1 cut(s)	479
BanI	GGYRCC	3 cut(s)	1090, 1502, 1864
BbsI	GAAGAC	2 cut(s)	813, 1566
Bbv12I	GWGCWC	1 cut(s)	399
BbvI	GCAGC	1 cut(s)	1348
BccI	CCATC	6 cut(s)	83, 395, 910, 1313, 1428, 1918
BceAI	ACGGC	1 cut(s)	976
BciT130I	CCWGG	7 cut(s)	436, 1065, 1098, 1224, 1538, 1750, 1797
BcnI	CCSGG	1 cut(s)	211
BcoDI	GTCTC	3 cut(s)	309, 1062, 1095
BfaI	CTAG	3 cut(s)	548, 812, 1545
BfmI	CTRYAG	1 cut(s)	582
BfoI	RGCGCY	1 cut(s)	792
BfrI	CTTAAG	1 cut(s)	1601
BfuAI	ACCTGC	2 cut(s)	654, 1262
BglII	AGATCT	1 cut(s)	276
BisI	GCNGC	1 cut(s)	1362
BlnI	CCTAGG	1 cut(s)	1544
BlsI	GCNGC	1 cut(s)	1363
Bme1390I	CCNGG	8 cut(s)	211, 436, 1065, 1098, 1224, 1538, 1750, 1797
Bme18I	GGWCC	7 cut(s)	34, 172, 673, 728, 857, 1322, 1907
BmgT120I	GGNCC	8 cut(s)	34, 172, 673, 728, 761, 857, 1322, 1907
BmiI	GGNNCC	8 cut(s)	173, 675, 763, 1092, 1504, 1542, 1819, 1866
BmrFI	CCNGG	8 cut(s)	211, 436, 1065, 1098, 1224, 1538, 1750, 1797
BmrI	ACTGGG	1 cut(s)	699
BmsI	GCATC	5 cut(s)	129, 880, 910, 1353, 1506
BmuI	ACTGGG	1 cut(s)	699
BpiI	GAAGAC	2 cut(s)	813, 1566
BpmI	CTGGAG	1 cut(s)	1086
Bpu14I	TTCGAA	3 cut(s)	1636, 1678, 1691
BpuEI	CTTGAG	1 cut(s)	291
BpuMI	CCSGG	1 cut(s)	211
Bsa29I	ATCGAT	2 cut(s)	523, 1243
BsaAI	YACGTR	1 cut(s)	1060
BsaBI	GATNNNNATC	1 cut(s)	1386
BsaJI	CCNNGG	6 cut(s)	839, 978, 1536, 1544, 1762, 1873
BsaWI	WCCGGW	2 cut(s)	514, 1718
Bsc4I	CCNNNNNNNGG	5 cut(s)	43, 683, 1070, 1319, 1874
Bse118I	RCCGGY	1 cut(s)	1773
Bse1I	ACTGG	5 cut(s)	705, 1138, 1506, 1529, 1929
Bse3DI	GCAATG	3 cut(s)	1015, 1659, 1802
Bse8I	GATNNNNATC	1 cut(s)	1386
BseAI	TCCGGA	1 cut(s)	514
BseBI	CCWGG	7 cut(s)	436, 1065, 1098, 1224, 1538, 1750, 1797
BseCI	ATCGAT	2 cut(s)	523, 1243
BseDI	CCNNGG	6 cut(s)	839, 978, 1536, 1544, 1762, 1873
BseGI	GGATG	5 cut(s)	616, 922, 1297, 1344, 1420
BseJI	GATNNNNATC	1 cut(s)	1386
BseLI	CCNNNNNNNGG	5 cut(s)	43, 683, 1070, 1319, 1874
BseMI	GCAATG	3 cut(s)	1015, 1659, 1802
BseMII	CTCAG	2 cut(s)	466, 522
BseNI	ACTGG	5 cut(s)	705, 1138, 1506, 1529, 1929
BseSI	GKGCMC	1 cut(s)	206
BseXI	GCAGC	1 cut(s)	1348
Bsh1236I	CGCG	1 cut(s)	1191
Bsh1285I	CGRYCG	2 cut(s)	523, 1719
BshFI	GGCC	4 cut(s)	412, 479, 762, 958
BshNI	GGYRCC	3 cut(s)	1090, 1502, 1864
BshVI	ATCGAT	2 cut(s)	523, 1243
BsiEI	CGRYCG	2 cut(s)	523, 1719
BsiHKAI	GWGCWC	1 cut(s)	399
BsiSI	CCGG	4 cut(s)	210, 515, 1719, 1774
BslFI	GGGAC	4 cut(s)	185, 226, 247, 308
BslI	CCNNNNNNNGG	5 cut(s)	43, 683, 1070, 1319, 1874
BsmAI	GTCTC	3 cut(s)	309, 1062, 1095
BsmBI	CGTCTC	3 cut(s)	309, 1062, 1095
BsmFI	GGGAC	4 cut(s)	185, 226, 247, 308
BsnI	GGCC	4 cut(s)	412, 479, 762, 958
Bsp119I	TTCGAA	3 cut(s)	1636, 1678, 1691
Bsp1286I	GDGCHC	2 cut(s)	206, 399
Bsp13I	TCCGGA	1 cut(s)	514
Bsp19I	CCATGG	2 cut(s)	839, 1762
BspACI	CCGC	3 cut(s)	989, 1269, 1706
BspANI	GGCC	4 cut(s)	412, 479, 762, 958
BspCNI	CTCAG	2 cut(s)	465, 523
BspDI	ATCGAT	2 cut(s)	523, 1243
BspEI	TCCGGA	1 cut(s)	514
BspFNI	CGCG	1 cut(s)	1191
BspLI	GGNNCC	8 cut(s)	173, 675, 763, 1092, 1504, 1542, 1819, 1866
BspMI	ACCTGC	2 cut(s)	654, 1262
BspPI	GGATC	4 cut(s)	572, 893, 1117, 1376
BspT104I	TTCGAA	3 cut(s)	1636, 1678, 1691
BspT107I	GGYRCC	3 cut(s)	1090, 1502, 1864
BspTI	CTTAAG	1 cut(s)	1601
BsrDI	GCAATG	3 cut(s)	1015, 1659, 1802
BsrFI	RCCGGY	1 cut(s)	1773
BsrI	ACTGG	5 cut(s)	705, 1138, 1506, 1529, 1929
BssAI	RCCGGY	1 cut(s)	1773
BssECI	CCNNGG	6 cut(s)	839, 978, 1536, 1544, 1762, 1873
BssT1I	CCWWGG	3 cut(s)	839, 1544, 1762
Bst2UI	CCWGG	7 cut(s)	436, 1065, 1098, 1224, 1538, 1750, 1797
Bst4CI	ACNGT	4 cut(s)	38, 116, 1073, 1258
BstAFI	CTTAAG	1 cut(s)	1601
BstAPI	GCANNNNNTGC	1 cut(s)	1661
BstBAI	YACGTR	1 cut(s)	1060
BstBI	TTCGAA	3 cut(s)	1636, 1678, 1691
BstC8I	GCNNGC	1 cut(s)	956
BstDEI	CTNAG	3 cut(s)	452, 531, 785
BstDSI	CCRYGG	3 cut(s)	839, 1762, 1873
BstF5I	GGATG	5 cut(s)	616, 922, 1297, 1344, 1420
BstFNI	CGCG	1 cut(s)	1191
BstH2I	RGCGCY	1 cut(s)	792
BstHHI	GCGC	1 cut(s)	791
BstMAI	GTCTC	3 cut(s)	309, 1062, 1095
BstMCI	CGRYCG	2 cut(s)	523, 1719
BstMWI	GCNNNNNNNGC	4 cut(s)	700, 768, 955, 1661
BstNI	CCWGG	7 cut(s)	436, 1065, 1098, 1224, 1538, 1750, 1797
BstNSI	RCATGY	2 cut(s)	508, 653
BstSCI	CCNGG	8 cut(s)	209, 434, 1063, 1096, 1222, 1536, 1748, 1795
BstSFI	CTRYAG	1 cut(s)	582
BstSLI	GKGCMC	1 cut(s)	206
BstUI	CGCG	1 cut(s)	1191
BstV1I	GCAGC	1 cut(s)	1348
BstV2I	GAAGAC	2 cut(s)	813, 1566
BstX2I	RGATCY	1 cut(s)	276
BstXI	CCANNNNNNTGG	1 cut(s)	1332
BstYI	RGATCY	1 cut(s)	276
Bsu15I	ATCGAT	2 cut(s)	523, 1243
BsuRI	GGCC	4 cut(s)	412, 479, 762, 958
BsuTUI	ATCGAT	2 cut(s)	523, 1243
BtgI	CCRYGG	3 cut(s)	839, 1762, 1873
BtgZI	GCGATG	1 cut(s)	791
BtsCI	GGATG	5 cut(s)	616, 922, 1297, 1344, 1420
BtsI	GCAGTG	1 cut(s)	1650
BtsIMutI	CAGTG	5 cut(s)	121, 712, 1513, 1618, 1650
BveI	ACCTGC	2 cut(s)	654, 1262
Cac8I	GCNNGC	1 cut(s)	956
CaiI	CAGNNNCTG	1 cut(s)	1033
CfoI	GCGC	1 cut(s)	791
Cfr10I	RCCGGY	1 cut(s)	1773
Cfr13I	GGNCC	8 cut(s)	34, 172, 673, 728, 761, 857, 1322, 1907
ClaI	ATCGAT	2 cut(s)	523, 1243
CseI	GACGC	1 cut(s)	1259
Csp6I	GTAC	4 cut(s)	1061, 1441, 1771, 1932
CspCI	CAANNNNNGTGG	2 cut(s)	754, 789
CviQI	GTAC	4 cut(s)	1061, 1441, 1771, 1932
DdeI	CTNAG	3 cut(s)	452, 531, 785
EaeI	YGGCCR	1 cut(s)	477
EciI	GGCGGA	1 cut(s)	978
Eco130I	CCWWGG	3 cut(s)	839, 1544, 1762
Eco32I	GATATC	1 cut(s)	1460
Eco47I	GGWCC	7 cut(s)	34, 172, 673, 728, 857, 1322, 1907
Eco47III	AGCGCT	1 cut(s)	790
Eco57I	CTGAAG	3 cut(s)	351, 579, 839
EcoO109I	RGGNCCY	1 cut(s)	172
EcoRI	GAATTC	2 cut(s)	593, 1367
EcoRII	CCWGG	7 cut(s)	434, 1063, 1096, 1222, 1536, 1748, 1795
EcoRV	GATATC	1 cut(s)	1460
EcoT14I	CCWWGG	3 cut(s)	839, 1544, 1762
EcoT22I	ATGCAT	1 cut(s)	1216
ErhI	CCWWGG	3 cut(s)	839, 1544, 1762
Esp3I	CGTCTC	3 cut(s)	309, 1062, 1095
FalI	AAGNNNNNCTT	4 cut(s)	552, 584, 943, 975
FaqI	GGGAC	4 cut(s)	185, 226, 247, 308
FauNDI	CATATG	1 cut(s)	382
Fnu4HI	GCNGC	1 cut(s)	1362
FokI	GGATG	5 cut(s)	623, 929, 1304, 1331, 1407
Fsp4HI	GCNGC	1 cut(s)	1362
FspBI	CTAG	3 cut(s)	548, 812, 1545
GlaI	GCGC	1 cut(s)	790
GluI	GCNGC	1 cut(s)	1362
GsuI	CTGGAG	1 cut(s)	1086
HaeII	RGCGCY	1 cut(s)	792
HaeIII	GGCC	4 cut(s)	412, 479, 762, 958
HapII	CCGG	4 cut(s)	210, 515, 1719, 1774
HgaI	GACGC	1 cut(s)	1259
HhaI	GCGC	1 cut(s)	791
Hin6I	GCGC	1 cut(s)	789
HinP1I	GCGC	1 cut(s)	789
HincII	GTYRAC	1 cut(s)	1417
HindII	GTYRAC	1 cut(s)	1417
HinfI	GANTC	8 cut(s)	298, 637, 742, 1030, 1675, 1688, 1758, 1878
HpaII	CCGG	4 cut(s)	210, 515, 1719, 1774
HphI	GGTGA	4 cut(s)	519, 739, 897, 1208
Hpy166II	GTNNAC	3 cut(s)	728, 1417, 1713
Hpy188I	TCNGA	4 cut(s)	455, 889, 979, 997
Hpy8I	GTNNAC	3 cut(s)	728, 1417, 1713
HpyAV	CCTTC	7 cut(s)	292, 434, 454, 521, 578, 1808, 1898
HpyCH4III	ACNGT	4 cut(s)	38, 116, 1073, 1258
HpyCH4IV	ACGT	4 cut(s)	74, 1059, 1104, 1328
HpyCH4V	TGCA	7 cut(s)	649, 893, 923, 949, 1214, 1655, 1664
HpyF10VI	GCNNNNNNNGC	4 cut(s)	700, 768, 955, 1661
HpyF3I	CTNAG	3 cut(s)	452, 531, 785
HpySE526I	ACGT	4 cut(s)	74, 1059, 1104, 1328
HspAI	GCGC	1 cut(s)	789
Kpn2I	TCCGGA	1 cut(s)	514
LmnI	GCTCC	1 cut(s)	1817
Lsp1109I	GCAGC	1 cut(s)	1348
LweI	GCATC	5 cut(s)	129, 880, 910, 1353, 1506
MaeI	CTAG	3 cut(s)	548, 812, 1545
MaeII	ACGT	4 cut(s)	74, 1059, 1104, 1328
MaeIII	GTNAC	4 cut(s)	70, 97, 1196, 1508
MfeI	CAATTG	1 cut(s)	1743
MflI	RGATCY	1 cut(s)	276
MhlI	GDGCHC	2 cut(s)	206, 399
MlsI	TGGCCA	1 cut(s)	479
MluNI	TGGCCA	1 cut(s)	479
MlyI	GAGTC	1 cut(s)	1872
MmeI	TCCRAC	1 cut(s)	1087
Mox20I	TGGCCA	1 cut(s)	479
Mph1103I	ATGCAT	1 cut(s)	1216
MroI	TCCGGA	1 cut(s)	514
MroXI	GAANNNNTTC	1 cut(s)	465
MscI	TGGCCA	1 cut(s)	479
MseI	TTAA	5 cut(s)	228, 876, 1014, 1487, 1602
MslI	CAYNNNNRTG	2 cut(s)	654, 1611
Msp20I	TGGCCA	1 cut(s)	479
MspA1I	CMGCKG	1 cut(s)	1708
MspCI	CTTAAG	1 cut(s)	1601
MspI	CCGG	4 cut(s)	210, 515, 1719, 1774
MspR9I	CCNGG	8 cut(s)	211, 436, 1065, 1098, 1224, 1538, 1750, 1797
MunI	CAATTG	1 cut(s)	1743
MvaI	CCWGG	7 cut(s)	436, 1065, 1098, 1224, 1538, 1750, 1797
MvnI	CGCG	1 cut(s)	1191
MwoI	GCNNNNNNNGC	4 cut(s)	700, 768, 955, 1661
NciI	CCSGG	1 cut(s)	211
NcoI	CCATGG	2 cut(s)	839, 1762
NdeI	CATATG	1 cut(s)	382
NlaIV	GGNNCC	8 cut(s)	173, 675, 763, 1092, 1504, 1542, 1819, 1866
NmuCI	GTSAC	2 cut(s)	1196, 1508
NsiI	ATGCAT	1 cut(s)	1216
NspI	RCATGY	2 cut(s)	508, 653
NspV	TTCGAA	3 cut(s)	1636, 1678, 1691
PdmI	GAANNNNTTC	1 cut(s)	465
PfeI	GAWTC	7 cut(s)	298, 637, 742, 1030, 1675, 1688, 1758
PflMI	CCANNNNNTGG	2 cut(s)	683, 1319
PkrI	GCNGC	1 cut(s)	1363
Ple19I	CGATCG	1 cut(s)	523
PleI	GAGTC	1 cut(s)	1872
PpsI	GAGTC	1 cut(s)	1872
Ppu21I	YACGTR	1 cut(s)	1060
PpuMI	RGGWCCY	1 cut(s)	172
PsiI	TTATAA	1 cut(s)	350
Psp1406I	AACGTT	1 cut(s)	1328
Psp5II	RGGWCCY	1 cut(s)	172
Psp6I	CCWGG	7 cut(s)	434, 1063, 1096, 1222, 1536, 1748, 1795
PspGI	CCWGG	7 cut(s)	434, 1063, 1096, 1222, 1536, 1748, 1795
PspN4I	GGNNCC	8 cut(s)	173, 675, 763, 1092, 1504, 1542, 1819, 1866
PspPI	GGNCC	8 cut(s)	34, 172, 673, 728, 761, 857, 1322, 1907
PspPPI	RGGWCCY	1 cut(s)	172
PstNI	CAGNNNCTG	1 cut(s)	1033
PsuI	RGATCY	1 cut(s)	276
PvuI	CGATCG	1 cut(s)	523
RsaI	GTAC	4 cut(s)	1062, 1442, 1772, 1933
RsaNI	GTAC	4 cut(s)	1061, 1441, 1771, 1932
RseI	CAYNNNNRTG	2 cut(s)	654, 1611
SaqAI	TTAA	5 cut(s)	228, 876, 1014, 1487, 1602
SatI	GCNGC	1 cut(s)	1362
Sau96I	GGNCC	8 cut(s)	34, 172, 673, 728, 761, 857, 1322, 1907
SchI	GAGTC	1 cut(s)	1872
ScrFI	CCNGG	8 cut(s)	211, 436, 1065, 1098, 1224, 1538, 1750, 1797
SduI	GDGCHC	2 cut(s)	206, 399
SfaNI	GCATC	5 cut(s)	129, 880, 910, 1353, 1506
SfcI	CTRYAG	1 cut(s)	582
SfuI	TTCGAA	3 cut(s)	1636, 1678, 1691
SinI	GGWCC	7 cut(s)	34, 172, 673, 728, 857, 1322, 1907
SmiMI	CAYNNNNRTG	2 cut(s)	654, 1611
SmlI	CTYRAG	3 cut(s)	9, 306, 1601
SmoI	CTYRAG	3 cut(s)	9, 306, 1601
SsiI	CCGC	3 cut(s)	989, 1269, 1706
SspMI	CTAG	3 cut(s)	548, 812, 1545
StyD4I	CCNGG	8 cut(s)	209, 434, 1063, 1096, 1222, 1536, 1748, 1795
StyI	CCWWGG	3 cut(s)	839, 1544, 1762
TaaI	ACNGT	4 cut(s)	38, 116, 1073, 1258
TaiI	ACGT	4 cut(s)	77, 1062, 1107, 1331
TfiI	GAWTC	7 cut(s)	298, 637, 742, 1030, 1675, 1688, 1758
Tru1I	TTAA	5 cut(s)	228, 876, 1014, 1487, 1602
Tru9I	TTAA	5 cut(s)	228, 876, 1014, 1487, 1602
TscAI	CASTG	5 cut(s)	121, 712, 1513, 1618, 1657
TseFI	GTSAC	2 cut(s)	1196, 1508
TseI	GCWGC	1 cut(s)	1361
Tsp45I	GTSAC	2 cut(s)	1196, 1508
TspDTI	ATGAA	7 cut(s)	162, 636, 644, 726, 856, 1494, 1572
TspGWI	ACGGA	3 cut(s)	291, 1458, 1890
TspRI	CASTG	5 cut(s)	121, 712, 1513, 1618, 1657
Van91I	CCANNNNNTGG	2 cut(s)	683, 1319
Vha464I	CTTAAG	1 cut(s)	1601
VpaK11BI	GGWCC	7 cut(s)	34, 172, 673, 728, 857, 1322, 1907
XapI	RAATTY	5 cut(s)	239, 593, 713, 849, 1367
XceI	RCATGY	2 cut(s)	508, 653
XcmI	CCANNNNNNNNNTGG	2 cut(s)	837, 909
XmaJI	CCTAGG	1 cut(s)	1544
XmnI	GAANNNNTTC	1 cut(s)	465
XspI	CTAG	3 cut(s)	548, 812, 1545
Zsp2I	ATGCAT	1 cut(s)	1216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.