Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002285

Physical Location & Seq

Genomic Coordinates Reverse (-)

12663066 .. 12673287

Gene Structure 10222 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001237

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGGCGTCCTCGTCCTCGCGCCAGAAGTACTTGTACAAAACGACGACTTTGGGACAGCCGTGGAGAACGGCGGGCGCGGTAGACGTCGGAGTAGGCGCCGGATCTGACCCGGCTCGAGGATCTGGTACTTGGCGATTATCTCCGGTCGAGTGTGGATGCTCCAGCTCTTCGCCGGTTATAATGGATAACGGCATTCTCCAAGTTACACTATCAAAACCAGGTGGAATTGTAACTGGAATACAATATAATGGCATCAACAATTTGCTTGAAGTTCAGAATACAGAAACTAATAGAGGGTATTGGGACCTTGTTTGGAGTGAAGCAGGAAGTGTCGGAACAACCGGAACATTTGATGTAATTAAAGGAACAAAGTTCACCGTCATAGTGGAAAACAAGGAACAGCTAGAGATATCATTCACAAGGAACTGGAACCATAAGAACAAGCATGTCCCGCTAAATATAGACAAAAGGTTTATAATGCTTCGTAATTCGTCAGGCTTCTACTCGTATGCCATCTATGAGCACTTGAAAGAATGGCCTCCTTTTAACCTTCCCCAGACCAGGATAACATTCAAGCTCAGTCAAGAAAAGTTTCGGTACATGGCCATAGCGGATAACAAGCAAAGATACATGCCGCTTCCGGATGACCGATTACCAGACAGAAGCAAAGTTTTAGATGTTCCAGAGGCAGTTCTTCTTGTCCATCCCATAGAGCCAGAATTTGAGGGAGAGGTCGATGACAAATATCAATACTCCTGCGAGAACCAGAATCTGCAAGTGCATGGTTGGATATGCATGGATCCACCCGTCGGGTTCTGGCAAATAACACCCAGCAATGAGTTCCGATCTGGTGGACCTCTTAAACAGAACCTCACCTCCCATGTTGGCCCAACTTGTCTTGCAATGTTTCTCAGTGCTCACTATTCAGGAGAGGACCTTGTGCTAAAGCTCAAACCAGATGAGCCATGGAAGAAGGTGTTTGGCCCTATTTTTATCTATCTTAATTCGGCGCCGGATGCAAAAGACTCGTTGTCACTCTGGGAGGATGCAAAGGCACAGATGATGACAGAAGTACAAAGTTGGCCGTACGAGTTTCCAGCATCTCATGACTTTCCACCATCAGATCAACGGGGTAATCTTAGTGGCAGAATACGCGTTCAAGATAGGTACGTAAGCGATACTTATATACTCGCCAACGGTGCTTATGTGGGCTTGGCACCTCCGGGAGACGCTGGATCATGGCAGAGAGATTGCAAGGGCTACCAGTTCTGGACTAGGGCAGATCAGAATGGTTATTACTCTATAAATGATATACGTGAGGGCAACTACGATCTTTATGCATGGGTTCCTGGTTTTATAGGAGATTATCGATATGATGAAACCATTAACGTAACCGCAGGTTGTGATATTGACATTGGTGATATTGTATATGAACCACCAAGGAGTGGACCAACATTGTGGGAAATTGGCATCCCTGATCGTTCTGCTGCTGAATTTTATGTCCCAGATCCTAATCCAAAGTATATCAATAAGCTATACGTCAACCATCCTGACAGGTTTAGGCAGTATGGTTTATGGGAAAGATATGCAGACTTATATCCTGAGGAGGATTTAGTGTTTACAGTTGGTGTTAGTGATTATACTAGAGACTGGTTCTTTGCTCACGTTACCAGGAAGAAGGATGATGGCAAGTATCAAGGCACAACGTGGCAAATAAAGTTTCGACTTGACAATGTCAGTCAGACCAAAACATACACACTTCGAATTGCCCTTGCTACTGCGAACGTTGAAGAACTACAGATTCGCGTAAATGATTCCAAGACAGATTCACCTGTATTTACGACCGGTGTGATCGGACATGACAACACAATTTGCAGACATGGAATTCATGGTCTCTACCGGCTGTATACAATAGACGTACCCGGCACATTGCTTGTGGAAGGAGACAACATAGTGTTTCTAACCCAAGCAATGAGCACCAGCCCTTTCCAGGGACTCATGTATGACTATATCCGTTTAGAAGGTCCATCAACTTCTATTTCTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

683

Amino Acids

77.33

Weight (kDa)

5.33

Isoelectric Point (pI)

37.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhamnogal_lyase	PF06045	57 - 245	1.6e-85	Rhamnogalacturonate lyase family
fn3_3	PF14686	399 - 471	6e-27	Polysaccharide lyase family 4, domain II
CBM-like	PF14683	485 - 673	1.1e-53	Polysaccharide lyase family 4, domain III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24430 AT4G24430
fragaria_vesca	FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34150
malus_domestica	MD08G1204400.v1.1 MD08G1205000.v1.1
prunus_persica	Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536700_v2.0.a1 Prupe.1G536700_v2.0.a1
pyrus_communis	pycom08g17670 pycom08g17720
rosa_chinensis	RchiOBHm_Chr7g0233711 RchiOBHm_Chr7g0233721 RchiOBHm_Chr7g0233731 RchiOBHm_Chr7g0233741
rosa_laevigata	RLG00000001237 RLG00000001239 RLG00000001240
rosa_multiflora	Rmu_sc0000740.1_g000047 Rmu_sc0000740.1_g000058 Rmu_sc0010145.1_g000001 Rmu_sc0011583.1_g000004
rosa_roxburghii	Rroxscaffold_3G00227030 Rroxscaffold_3G00227050 Rroxscaffold_3G00227070
rosa_rugosa	Rorug07G0282300 Rorug07G0282300
rosa_samantha	Rh7AG438600 Rh7AG438700 Rh7BG411300 Rh7CG458200 Rh7CG458300 Rh7CG458400 Rh7DG428200
rosa_wichuraiana	Rw7G036170 Rw7G036180 Rw7G036230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	179, 476
AatII	GACGTC	1 cut(s)	87
Acc36I	ACCTGC	1 cut(s)	1388
AccB1I	GGYRCC	3 cut(s)	95, 1009, 1216
AccB7I	CCANNNNNTGG	1 cut(s)	1445
AccI	GTMKAC	2 cut(s)	81, 1907
AccII	CGCG	4 cut(s)	19, 77, 1155, 1806
AccIII	TCCGGA	1 cut(s)	640
AciI	CCGC	6 cut(s)	71, 77, 452, 611, 635, 1395
AclI	AACGTT	1 cut(s)	1785
AclWI	GGATC	6 cut(s)	109, 127, 794, 807, 1243, 1502
AcoI	YGGCCR	2 cut(s)	603, 1082
AcsI	RAATTY	3 cut(s)	719, 1493, 1884
AcyI	GRCGYC	4 cut(s)	5, 84, 96, 1010
AdeI	CACNNNGTG	1 cut(s)	1707
AfaI	GTAC	8 cut(s)	29, 35, 127, 599, 1074, 1088, 1169, 1920
AfiI	CCNNNNNNNGG	6 cut(s)	116, 561, 809, 1445, 1990, 1991
AflIII	ACRYGT	1 cut(s)	1153
AgeI	ACCGGT	1 cut(s)	1844
AgsI	TTSAA	5 cut(s)	269, 529, 574, 1160, 1790
AhdI	GACNNNNNGTC	1 cut(s)	1030
AjnI	CCWGG	5 cut(s)	217, 560, 1348, 1670, 1989
AloI	GAACNNNNNNTCC	2 cut(s)	860, 892
AluBI	AGCT	5 cut(s)	165, 403, 577, 949, 1534
AluI	AGCT	5 cut(s)	165, 403, 577, 949, 1534
Alw21I	GWGCWC	3 cut(s)	525, 919, 1979
Alw26I	GTCTC	4 cut(s)	1221, 1641, 1898, 1938
AlwI	GGATC	6 cut(s)	109, 127, 794, 807, 1243, 1502
AlwNI	CAGNNNCTG	1 cut(s)	772
Ama87I	CYCGRG	1 cut(s)	114
Aor13HI	TCCGGA	1 cut(s)	640
AoxI	GGCC	5 cut(s)	536, 603, 886, 982, 1082
ApeKI	GCWGC	1 cut(s)	1487
ApoI	RAATTY	3 cut(s)	719, 1493, 1884
ArsI	GACNNNNNNTTYG	2 cut(s)	1640, 1672
AsiGI	ACCGGT	1 cut(s)	1844
Asp700I	GAANNNNTTC	1 cut(s)	591
AspLEI	GCGC	4 cut(s)	21, 77, 98, 1012
AspS9I	GGNCC	7 cut(s)	304, 854, 887, 934, 983, 1448, 2024
AsuC2I	CCSGG	3 cut(s)	110, 1224, 1923
AsuHPI	GGTGA	4 cut(s)	367, 865, 1430, 1821
AsuII	TTCGAA	1 cut(s)	1762
AvaI	CYCGRG	1 cut(s)	114
AvaII	GGWCC	5 cut(s)	304, 854, 934, 1448, 2024
AxyI	CCTNAGG	1 cut(s)	1602
BalI	TGGCCA	1 cut(s)	605
BamHI	GGATCC	1 cut(s)	799
BanI	GGYRCC	3 cut(s)	95, 1009, 1216
BarI	GAAGNNNNNNTAC	2 cut(s)	17, 49
Bbv12I	GWGCWC	3 cut(s)	525, 919, 1979
BbvI	GCAGC	1 cut(s)	1474
BccI	CCATC	6 cut(s)	521, 711, 1126, 1554, 1679, 2035
BceAI	ACGGC	4 cut(s)	43, 84, 205, 1069
BcgI	CGANNNNNNTGC	4 cut(s)	1008, 1042, 1319, 1353
BciT130I	CCWGG	5 cut(s)	219, 562, 1350, 1672, 1991
BcnI	CCSGG	3 cut(s)	110, 1224, 1923
BcoDI	GTCTC	4 cut(s)	1221, 1641, 1898, 1938
BfaI	CTAG	3 cut(s)	404, 1275, 1644
BfmI	CTRYAG	1 cut(s)	1796
BfoI	RGCGCY	2 cut(s)	99, 1013
BfuAI	ACCTGC	1 cut(s)	1388
BisI	GCNGC	2 cut(s)	635, 1488
BlsI	GCNGC	2 cut(s)	636, 1489
BmcAI	AGTACT	1 cut(s)	29
Bme1390I	CCNGG	8 cut(s)	110, 219, 562, 1224, 1350, 1672, 1923, 1991
Bme18I	GGWCC	5 cut(s)	304, 854, 934, 1448, 2024
BmeRI	GACNNNNNGTC	1 cut(s)	1030
BmeT110I	CYCGRG	1 cut(s)	114
BmgT120I	GGNCC	7 cut(s)	304, 854, 887, 934, 983, 1448, 2024
BmiI	GGNNCC	7 cut(s)	97, 305, 431, 801, 1011, 1218, 1347
BmrFI	CCNGG	8 cut(s)	110, 219, 562, 1224, 1350, 1672, 1923, 1991
BmsI	GCATC	6 cut(s)	146, 261, 1006, 1036, 1109, 1479
BpmI	CTGGAG	1 cut(s)	145
Bpu14I	TTCGAA	1 cut(s)	1762
BpuMI	CCSGG	3 cut(s)	110, 1224, 1923
Bsa29I	ATCGAT	1 cut(s)	1369
BsaAI	YACGTR	2 cut(s)	1171, 1316
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	4 cut(s)	5, 84, 96, 1010
BsaI	GGTCTC	1 cut(s)	1898
BsaJI	CCNNGG	4 cut(s)	59, 965, 1439, 1990
BsaWI	WCCGGW	4 cut(s)	142, 341, 640, 1844
BsaXI	ACNNNNNCTCC	2 cut(s)	860, 890
Bsc4I	CCNNNNNNNGG	6 cut(s)	116, 561, 809, 1445, 1990, 1991
Bse118I	RCCGGY	3 cut(s)	172, 1844, 1899
Bse1I	ACTGG	4 cut(s)	238, 431, 1264, 1655
Bse21I	CCTNAGG	1 cut(s)	1602
Bse3DI	GCAATG	4 cut(s)	841, 909, 1928, 1977
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	1 cut(s)	640
BseBI	CCWGG	5 cut(s)	219, 562, 1350, 1672, 1991
BseCI	ATCGAT	1 cut(s)	1369
BseDI	CCNNGG	4 cut(s)	59, 965, 1439, 1990
BseGI	GGATG	8 cut(s)	161, 649, 703, 1021, 1051, 1470, 1546, 1687
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	6 cut(s)	116, 561, 809, 1445, 1990, 1991
BseMI	GCAATG	4 cut(s)	841, 909, 1928, 1977
BseMII	CTCAG	3 cut(s)	592, 925, 1593
BseNI	ACTGG	4 cut(s)	238, 431, 1264, 1655
BseRI	GAGGAG	1 cut(s)	1619
BseXI	GCAGC	1 cut(s)	1474
BseYI	CCCAGC	1 cut(s)	830
Bsh1236I	CGCG	4 cut(s)	19, 77, 1155, 1806
Bsh1285I	CGRYCG	2 cut(s)	147, 1845
BshFI	GGCC	5 cut(s)	538, 605, 888, 984, 1084
BshNI	GGYRCC	3 cut(s)	95, 1009, 1216
BshTI	ACCGGT	1 cut(s)	1844
BshVI	ATCGAT	1 cut(s)	1369
BsiEI	CGRYCG	2 cut(s)	147, 1845
BsiHKAI	GWGCWC	3 cut(s)	525, 919, 1979
BsiHKCI	CYCGRG	1 cut(s)	114
BsiWI	CGTACG	1 cut(s)	1086
BslFI	GGGAC	5 cut(s)	66, 317, 434, 1487, 2007
BslI	CCNNNNNNNGG	6 cut(s)	116, 561, 809, 1445, 1990, 1991
BsmAI	GTCTC	4 cut(s)	1221, 1641, 1898, 1938
BsmBI	CGTCTC	1 cut(s)	1221
BsmFI	GGGAC	5 cut(s)	66, 317, 434, 1487, 2007
BsmI	GAATGC	1 cut(s)	192
BsnI	GGCC	5 cut(s)	538, 605, 888, 984, 1084
Bso31I	GGTCTC	1 cut(s)	1898
BsoBI	CYCGRG	1 cut(s)	114
Bsp119I	TTCGAA	1 cut(s)	1762
Bsp1286I	GDGCHC	3 cut(s)	525, 919, 1979
Bsp13I	TCCGGA	1 cut(s)	640
Bsp1407I	TGTACA	1 cut(s)	33
Bsp19I	CCATGG	1 cut(s)	965
BspACI	CCGC	6 cut(s)	71, 77, 452, 611, 635, 1395
BspANI	GGCC	5 cut(s)	538, 605, 888, 984, 1084
BspCNI	CTCAG	3 cut(s)	591, 924, 1594
BspDI	ATCGAT	1 cut(s)	1369
BspEI	TCCGGA	1 cut(s)	640
BspFNI	CGCG	4 cut(s)	19, 77, 1155, 1806
BspHI	TCATGA	1 cut(s)	1105
BspLI	GGNNCC	7 cut(s)	97, 305, 431, 801, 1011, 1218, 1347
BspMI	ACCTGC	1 cut(s)	1388
BspPI	GGATC	6 cut(s)	109, 127, 794, 807, 1243, 1502
BspQI	GCTCTTC	1 cut(s)	172
BspT104I	TTCGAA	1 cut(s)	1762
BspT107I	GGYRCC	3 cut(s)	95, 1009, 1216
BspTNI	GGTCTC	1 cut(s)	1898
BsrDI	GCAATG	4 cut(s)	841, 909, 1928, 1977
BsrFI	RCCGGY	3 cut(s)	172, 1844, 1899
BsrGI	TGTACA	1 cut(s)	33
BsrI	ACTGG	4 cut(s)	238, 431, 1264, 1655
BssAI	RCCGGY	3 cut(s)	172, 1844, 1899
BssECI	CCNNGG	4 cut(s)	59, 965, 1439, 1990
BssNAI	GTATAC	1 cut(s)	1908
BssNI	GRCGYC	4 cut(s)	5, 84, 96, 1010
BssT1I	CCWWGG	2 cut(s)	965, 1439
Bst1107I	GTATAC	1 cut(s)	1908
Bst2UI	CCWGG	5 cut(s)	219, 562, 1350, 1672, 1991
Bst4CI	ACNGT	3 cut(s)	379, 1199, 1624
Bst6I	CTCTTC	1 cut(s)	172
BstACI	GRCGYC	4 cut(s)	5, 84, 96, 1010
BstAUI	TGTACA	1 cut(s)	33
BstBAI	YACGTR	2 cut(s)	1171, 1316
BstBI	TTCGAA	1 cut(s)	1762
BstC8I	GCNNGC	1 cut(s)	73
BstDEI	CTNAG	4 cut(s)	578, 911, 1139, 1602
BstDSI	CCRYGG	2 cut(s)	59, 965
BstF5I	GGATG	8 cut(s)	161, 649, 703, 1021, 1051, 1470, 1546, 1687
BstFNI	CGCG	4 cut(s)	19, 77, 1155, 1806
BstH2I	RGCGCY	2 cut(s)	99, 1013
BstHHI	GCGC	4 cut(s)	21, 77, 98, 1012
BstMAI	GTCTC	4 cut(s)	1221, 1641, 1898, 1938
BstMCI	CGRYCG	2 cut(s)	147, 1845
BstMWI	GCNNNNNNNGC	4 cut(s)	451, 1016, 1152, 1199
BstNI	CCWGG	5 cut(s)	219, 562, 1350, 1672, 1991
BstNSI	RCATGY	2 cut(s)	449, 634
BstSCI	CCNGG	8 cut(s)	108, 217, 560, 1222, 1348, 1670, 1921, 1989
BstSFI	CTRYAG	1 cut(s)	1796
BstSNI	TACGTA	1 cut(s)	1171
BstUI	CGCG	4 cut(s)	19, 77, 1155, 1806
BstV1I	GCAGC	1 cut(s)	1474
BstX2I	RGATCY	4 cut(s)	101, 119, 799, 1507
BstXI	CCANNNNNNTGG	1 cut(s)	1458
BstYI	RGATCY	4 cut(s)	101, 119, 799, 1507
BstZ17I	GTATAC	1 cut(s)	1908
Bsu15I	ATCGAT	1 cut(s)	1369
Bsu36I	CCTNAGG	1 cut(s)	1602
BsuRI	GGCC	5 cut(s)	538, 605, 888, 984, 1084
BsuTUI	ATCGAT	1 cut(s)	1369
BtgI	CCRYGG	2 cut(s)	59, 965
BtsCI	GGATG	8 cut(s)	161, 649, 703, 1021, 1051, 1470, 1546, 1687
BtsIMutI	CAGTG	1 cut(s)	919
BveI	ACCTGC	1 cut(s)	1388
Cac8I	GCNNGC	1 cut(s)	73
CaiI	CAGNNNCTG	1 cut(s)	772
CciI	TCATGA	1 cut(s)	1105
CfoI	GCGC	4 cut(s)	21, 77, 98, 1012
Cfr10I	RCCGGY	3 cut(s)	172, 1844, 1899
Cfr13I	GGNCC	7 cut(s)	304, 854, 887, 934, 983, 1448, 2024
ClaI	ATCGAT	1 cut(s)	1369
CseI	GACGC	1 cut(s)	1238
CsiI	ACCWGGT	1 cut(s)	217
Csp6I	GTAC	8 cut(s)	28, 34, 126, 598, 1073, 1087, 1168, 1919
CspAI	ACCGGT	1 cut(s)	1844
CviQI	GTAC	8 cut(s)	28, 34, 126, 598, 1073, 1087, 1168, 1919
DdeI	CTNAG	4 cut(s)	578, 911, 1139, 1602
DinI	GGCGCC	2 cut(s)	97, 1011
DraIII	CACNNNGTG	1 cut(s)	1707
DriI	GACNNNNNGTC	1 cut(s)	1030
EaeI	YGGCCR	2 cut(s)	603, 1082
Eam1104I	CTCTTC	1 cut(s)	172
Eam1105I	GACNNNNNGTC	1 cut(s)	1030
EarI	CTCTTC	1 cut(s)	172
Eco105I	TACGTA	1 cut(s)	1171
Eco130I	CCWWGG	2 cut(s)	965, 1439
Eco31I	GGTCTC	1 cut(s)	1898
Eco32I	GATATC	1 cut(s)	411
Eco47I	GGWCC	5 cut(s)	304, 854, 934, 1448, 2024
Eco81I	CCTNAGG	1 cut(s)	1602
Eco88I	CYCGRG	1 cut(s)	114
EcoO109I	RGGNCCY	2 cut(s)	304, 934
EcoRI	GAATTC	1 cut(s)	1884
EcoRII	CCWGG	5 cut(s)	217, 560, 1348, 1670, 1989
EcoRV	GATATC	1 cut(s)	411
EcoT14I	CCWWGG	2 cut(s)	965, 1439
EcoT22I	ATGCAT	2 cut(s)	797, 1342
EgeI	GGCGCC	2 cut(s)	97, 1011
EheI	GGCGCC	2 cut(s)	97, 1011
ErhI	CCWWGG	2 cut(s)	965, 1439
Esp3I	CGTCTC	1 cut(s)	1221
FalI	AAGNNNNNCTT	2 cut(s)	1165, 1197
FaqI	GGGAC	5 cut(s)	66, 317, 434, 1487, 2007
FauI	CCCGC	2 cut(s)	64, 459
FblI	GTMKAC	2 cut(s)	81, 1907
Fnu4HI	GCNGC	2 cut(s)	635, 1488
FokI	GGATG	8 cut(s)	168, 656, 690, 1028, 1058, 1457, 1533, 1694
Fsp4HI	GCNGC	2 cut(s)	635, 1488
FspBI	CTAG	3 cut(s)	404, 1275, 1644
GlaI	GCGC	4 cut(s)	20, 76, 97, 1011
GluI	GCNGC	2 cut(s)	635, 1488
GsaI	CCCAGC	1 cut(s)	834
GsuI	CTGGAG	1 cut(s)	145
HaeII	RGCGCY	2 cut(s)	99, 1013
HaeIII	GGCC	5 cut(s)	538, 605, 888, 984, 1084
HgaI	GACGC	1 cut(s)	1238
HhaI	GCGC	4 cut(s)	21, 77, 98, 1012
Hin1I	GRCGYC	4 cut(s)	5, 84, 96, 1010
Hin6I	GCGC	4 cut(s)	19, 75, 96, 1010
HinP1I	GCGC	4 cut(s)	19, 75, 96, 1010
HincII	GTYRAC	1 cut(s)	1543
HindII	GTYRAC	1 cut(s)	1543
HinfI	GANTC	6 cut(s)	769, 1025, 1801, 1814, 1826, 1995
HphI	GGTGA	4 cut(s)	367, 865, 1430, 1821
Hpy166II	GTNNAC	7 cut(s)	82, 375, 854, 1448, 1543, 1620, 1908
Hpy188I	TCNGA	9 cut(s)	89, 106, 276, 335, 845, 1123, 1287, 1743, 1856
Hpy188III	TCNNGA	9 cut(s)	584, 641, 683, 927, 1106, 1160, 1270, 1550, 1601
Hpy8I	GTNNAC	7 cut(s)	82, 375, 854, 1448, 1543, 1620, 1908
Hpy99I	CGWCG	3 cut(s)	46, 89, 812
HpyAV	CCTTC	5 cut(s)	560, 967, 1672, 1934, 2015
HpyCH4III	ACNGT	3 cut(s)	379, 1199, 1624
HpyCH4IV	ACGT	9 cut(s)	84, 1170, 1315, 1389, 1539, 1665, 1706, 1785, 1917
HpyF10VI	GCNNNNNNNGC	4 cut(s)	451, 1016, 1152, 1199
HpyF3I	CTNAG	4 cut(s)	578, 911, 1139, 1602
HpySE526I	ACGT	9 cut(s)	84, 1170, 1315, 1389, 1539, 1665, 1706, 1785, 1917
Hsp92I	GRCGYC	4 cut(s)	5, 84, 96, 1010
HspAI	GCGC	4 cut(s)	19, 75, 96, 1010
KasI	GGCGCC	2 cut(s)	95, 1009
Kpn2I	TCCGGA	1 cut(s)	640
LguI	GCTCTTC	1 cut(s)	172
LmnI	GCTCC	1 cut(s)	164
Lsp1109I	GCAGC	1 cut(s)	1474
LweI	GCATC	6 cut(s)	146, 261, 1006, 1036, 1109, 1479
MabI	ACCWGGT	1 cut(s)	217
MaeI	CTAG	3 cut(s)	404, 1275, 1644
MaeII	ACGT	9 cut(s)	84, 1170, 1315, 1389, 1539, 1665, 1706, 1785, 1917
MaeIII	GTNAC	5 cut(s)	202, 229, 1032, 1390, 1666
MboII	GAAGA	5 cut(s)	159, 686, 982, 1687, 1802
MflI	RGATCY	4 cut(s)	101, 119, 799, 1507
MhlI	GDGCHC	3 cut(s)	525, 919, 1979
MlsI	TGGCCA	1 cut(s)	605
MluI	ACGCGT	1 cut(s)	1153
MluNI	TGGCCA	1 cut(s)	605
Mly113I	GGCGCC	2 cut(s)	96, 1010
MlyI	GAGTC	2 cut(s)	1019, 1989
MmeI	TCCRAC	3 cut(s)	67, 313, 767
Mox20I	TGGCCA	1 cut(s)	605
Mph1103I	ATGCAT	2 cut(s)	797, 1342
MroI	TCCGGA	1 cut(s)	640
MroXI	GAANNNNTTC	1 cut(s)	591
MscI	TGGCCA	1 cut(s)	605
MseI	TTAA	5 cut(s)	360, 546, 861, 1002, 1386
Msp20I	TGGCCA	1 cut(s)	605
MspR9I	CCNGG	8 cut(s)	110, 219, 562, 1224, 1350, 1672, 1923, 1991
Mva1269I	GAATGC	1 cut(s)	192
MvaI	CCWGG	5 cut(s)	219, 562, 1350, 1672, 1991
MvnI	CGCG	4 cut(s)	19, 77, 1155, 1806
MwoI	GCNNNNNNNGC	4 cut(s)	451, 1016, 1152, 1199
NarI	GGCGCC	2 cut(s)	96, 1010
NciI	CCSGG	3 cut(s)	110, 1224, 1923
NcoI	CCATGG	1 cut(s)	965
NlaIV	GGNNCC	7 cut(s)	97, 305, 431, 801, 1011, 1218, 1347
NmuCI	GTSAC	1 cut(s)	1032
NsiI	ATGCAT	2 cut(s)	797, 1342
NspI	RCATGY	2 cut(s)	449, 634
NspV	TTCGAA	1 cut(s)	1762
PaeR7I	CTCGAG	1 cut(s)	114
PagI	TCATGA	1 cut(s)	1105
PciSI	GCTCTTC	1 cut(s)	172
PctI	GAATGC	1 cut(s)	192
PdmI	GAANNNNTTC	1 cut(s)	591
PfeI	GAWTC	4 cut(s)	769, 1801, 1814, 1826
Pfl23II	CGTACG	1 cut(s)	1086
PflFI	GACNNNGTC	1 cut(s)	1733
PflMI	CCANNNNNTGG	1 cut(s)	1445
PfoI	TCCNGGA	1 cut(s)	1222
PinAI	ACCGGT	1 cut(s)	1844
PkrI	GCNGC	2 cut(s)	636, 1489
PleI	GAGTC	2 cut(s)	1019, 1989
PluTI	GGCGCC	2 cut(s)	99, 1013
PpsI	GAGTC	2 cut(s)	1019, 1989
Ppu21I	YACGTR	2 cut(s)	1171, 1316
PpuMI	RGGWCCY	2 cut(s)	304, 934
PsiI	TTATAA	2 cut(s)	179, 476
Psp1406I	AACGTT	1 cut(s)	1785
Psp5II	RGGWCCY	2 cut(s)	304, 934
Psp6I	CCWGG	5 cut(s)	217, 560, 1348, 1670, 1989
PspFI	CCCAGC	1 cut(s)	830
PspGI	CCWGG	5 cut(s)	217, 560, 1348, 1670, 1989
PspLI	CGTACG	1 cut(s)	1086
PspN4I	GGNNCC	7 cut(s)	97, 305, 431, 801, 1011, 1218, 1347
PspPI	GGNCC	7 cut(s)	304, 854, 887, 934, 983, 1448, 2024
PspPPI	RGGWCCY	2 cut(s)	304, 934
PspXI	VCTCGAGB	1 cut(s)	114
PstNI	CAGNNNCTG	1 cut(s)	772
PsuI	RGATCY	4 cut(s)	101, 119, 799, 1507
PsyI	GACNNNGTC	1 cut(s)	1733
RsaI	GTAC	8 cut(s)	29, 35, 127, 599, 1074, 1088, 1169, 1920
RsaNI	GTAC	8 cut(s)	28, 34, 126, 598, 1073, 1087, 1168, 1919
SapI	GCTCTTC	1 cut(s)	172
SaqAI	TTAA	5 cut(s)	360, 546, 861, 1002, 1386
SatI	GCNGC	2 cut(s)	635, 1488
Sau96I	GGNCC	7 cut(s)	304, 854, 887, 934, 983, 1448, 2024
ScaI	AGTACT	1 cut(s)	29
SchI	GAGTC	2 cut(s)	1019, 1989
ScrFI	CCNGG	8 cut(s)	110, 219, 562, 1224, 1350, 1672, 1923, 1991
SduI	GDGCHC	3 cut(s)	525, 919, 1979
SexAI	ACCWGGT	1 cut(s)	217
SfaNI	GCATC	6 cut(s)	146, 261, 1006, 1036, 1109, 1479
SfcI	CTRYAG	1 cut(s)	1796
SfoI	GGCGCC	2 cut(s)	97, 1011
Sfr274I	CTCGAG	1 cut(s)	114
SfuI	TTCGAA	1 cut(s)	1762
SinI	GGWCC	5 cut(s)	304, 854, 934, 1448, 2024
SlaI	CTCGAG	1 cut(s)	114
SmlI	CTYRAG	1 cut(s)	114
SmoI	CTYRAG	1 cut(s)	114
SnaBI	TACGTA	1 cut(s)	1171
SsiI	CCGC	6 cut(s)	71, 77, 452, 611, 635, 1395
SspDI	GGCGCC	2 cut(s)	95, 1009
SspMI	CTAG	3 cut(s)	404, 1275, 1644
StyD4I	CCNGG	8 cut(s)	108, 217, 560, 1222, 1348, 1670, 1921, 1989
StyI	CCWWGG	2 cut(s)	965, 1439
TaaI	ACNGT	3 cut(s)	379, 1199, 1624
TaiI	ACGT	9 cut(s)	87, 1173, 1318, 1392, 1542, 1668, 1709, 1788, 1920
TaqI	TCGA	6 cut(s)	115, 147, 735, 1369, 1723, 1762
TaqII	GACCGA	1 cut(s)	663
TatI	WGTACW	3 cut(s)	27, 33, 1072
TauI	GCSGC	1 cut(s)	637
TfiI	GAWTC	4 cut(s)	769, 1801, 1814, 1826
Tru1I	TTAA	5 cut(s)	360, 546, 861, 1002, 1386
Tru9I	TTAA	5 cut(s)	360, 546, 861, 1002, 1386
TscAI	CASTG	1 cut(s)	919
TseFI	GTSAC	1 cut(s)	1032
TseI	GCWGC	1 cut(s)	1487
Tsp45I	GTSAC	1 cut(s)	1032
TspDTI	ATGAA	3 cut(s)	1392, 1446, 1877
TspGWI	ACGGA	1 cut(s)	2003
TspRI	CASTG	1 cut(s)	919
Tth111I	GACNNNGTC	1 cut(s)	1733
Van91I	CCANNNNNTGG	1 cut(s)	1445
VpaK11BI	GGWCC	5 cut(s)	304, 854, 934, 1448, 2024
XapI	RAATTY	3 cut(s)	719, 1493, 1884
XceI	RCATGY	2 cut(s)	449, 634
XcmI	CCANNNNNNNNNTGG	1 cut(s)	963
XhoI	CTCGAG	1 cut(s)	114
XmiI	GTMKAC	2 cut(s)	81, 1907
XmnI	GAANNNNTTC	1 cut(s)	591
XspI	CTAG	3 cut(s)	404, 1275, 1644
ZraI	GACGTC	1 cut(s)	85
ZrmI	AGTACT	1 cut(s)	29
Zsp2I	ATGCAT	2 cut(s)	797, 1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.