Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0002285

Physical Location & Seq

Genomic Coordinates Forward (+)

60413087 .. 60427167

Gene Structure 14081 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG458400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGACTTGCAGAAAAGTAATTGTATTCGGCCTCCAAATCCACACTAACATTTCAAAGGGAGTCCTCACAATTGCAATGTCGTCCCAAGCTGTGCAATTGCATATTCAGGATCATCATGTTATAATGGATAACGGCATACTCCAAGTTACACTGTCAAAACCAGGTGGAATTGTAACTGGAATACAATATAATGGCATCAACAATTTGCTTGAAGTTCAGAATACAGAAACTAATAGAGGGTATTGGGACCTTGTTTGGAGTGAAGCAGGAAGTGTCGGAACAACCGGAACATTTGATGTAATTAAAGGAACAAAGTTCACCGTCATAGTGGAAAACGAGGAACAGGTAGAGATATCATTCACAAGGAACTGGAACCATAAGAACAAGCATGTCCCGCTAAATATAGACAAAAGGTTTATAATGCTTCGTAATTCGTCAGGCTTCTACTCGTATGCCATCTATGAGCACTTGAAAGAATGGCCTCCTTTTAACCTTCCGCAGACCAGGATAACATTCAAGCTCAGTCAAGAAAAGTTTCGGTACATGGCCATAGCGGATAACAGGCAAAGATACATGCCGCTTCCGGATGACCGATTACCAGACAGAAGCAAAGTTTTAGATTTTCCAGAGGCAGTTCTTCTTGTCCATCCCATAGAGCCAGAATTTGAGGGAGAGGTCGATGACAAATATCAATACTCCTGCGAGAACCAGAATCTGCAAGTGCATGGTTGGATATGCATGGATCCACCCGTCGGGTTCTGGCAAATAACACCCAGCAATGAGTTCCGATCTGGTGGACCTCTTAAACAGAACCTCACCTCCCATGTTGGCCCAACTTGTCTTGCAATGTTTCTCAGTGCTCACTATTCAGGAGAGGACCTTGTGCTAAAGCTCAAACCAGATGAGCCATGGAAGAAGGTGTTTGGCCCTATTTTTATCTATCTTAATTCGGCGCCGGATGCAAAAGACTCGTTGTCACTCTGGGAGGATGCAAAGGCACAGATGATGACAGAAGTACAAAGTTGGCCGTACGAGTTTCCAGCATCTCATGACTTTCCACCATCAGATCAACGGGGTAATCTTAGTGGCAGAATACGCGTTCAAGATAGGTACGTAAGCGATACTTATATACTCGCCAACGGTGCTTACGTGGGCTTGGCACCTCCGGGAGACGCTGGATCATGGCAGAGAGATTGCAAGGGCTACCAGTTCTGGACTAGGGCAGATCAGAATGGTTATTACTCTATAAATGATATACGTGAGGGCAACTACGATCTTTATGCATGGGTTCCTGGTTTTATAGGAGATTATCGATATGATGAAACCATTAACGTAACCGCAGGTTGTGATATTGACATTGGTGATATTGTATATGAACCACCAAGGAGTGGACCAACATTGTGGGAAATTGGCATCCCTGATCGTTCTGCTGCTGAATTTTATGTCCCAGATCCTAATCCAAAGTATATCAATAAGCTATATGTCAACCATCCTGACAGGTTTAGGCAGTATGGTTTATGGGAAAGATATGCAGACTTATATCCTGAGGAGGATTTAGTGTTTACAGTTGGTGTTAGTGATTATACTAGAGACTGGTTCTTTGCTCACGTTACCAGGAAGAAGGATGATGGCAAGTATCAAAGCACTACGTGGCAAATAAAGTTTCGACTTGACAATGTCAGTCAGACCAAAACATACACACTTCGAATTGCCCTTGCTACTGCAAACGTTGCAGAACTACAGATTCGCGTAAATGATTCCAAGACAGATTCACCTGTATTTACGACCGGTGTGATCGGACACGACAACACAATTTGCAGACATGGAATTCATGGTCTCTACCGGCTGTATACAATAGACGTACCCGGCACATTGCTTGTGGAAGGAGACAACATAGTGTTTCTAACCCAAGCAATGAGCACCAGCCCTTTCCAGGGACTCATGTATGACTATATCCGTTTAGAAGGTCCATCAACTTCTATTTCTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

663

Amino Acids

75.77

Weight (kDa)

5.39

Isoelectric Point (pI)

36.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhamnogal_lyase	PF06045	23 - 226	3.6e-91	Rhamnogalacturonate lyase family
fn3_3	PF14686	380 - 452	5.7e-27	Polysaccharide lyase family 4, domain II
CBM-like	PF14683	466 - 654	4.9e-54	Polysaccharide lyase family 4, domain III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24430 AT4G24430
fragaria_vesca	FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34150
malus_domestica	MD08G1204400.v1.1 MD08G1205000.v1.1
prunus_persica	Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536700_v2.0.a1 Prupe.1G536700_v2.0.a1
pyrus_communis	pycom08g17670 pycom08g17720
rosa_chinensis	RchiOBHm_Chr7g0233711 RchiOBHm_Chr7g0233721 RchiOBHm_Chr7g0233731 RchiOBHm_Chr7g0233741
rosa_laevigata	RLG00000001237 RLG00000001239 RLG00000001240
rosa_multiflora	Rmu_sc0000740.1_g000047 Rmu_sc0000740.1_g000058 Rmu_sc0010145.1_g000001 Rmu_sc0011583.1_g000004
rosa_roxburghii	Rroxscaffold_3G00227030 Rroxscaffold_3G00227050 Rroxscaffold_3G00227070
rosa_rugosa	Rorug07G0282300 Rorug07G0282300
rosa_samantha	Rh7AG438600 Rh7AG438700 Rh7BG411300 Rh7CG458200 Rh7CG458300 Rh7CG458400 Rh7DG428200
rosa_wichuraiana	Rw7G036170 Rw7G036180 Rw7G036230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	122, 419
Acc36I	ACCTGC	1 cut(s)	1331
AccB1I	GGYRCC	2 cut(s)	952, 1159
AccB7I	CCANNNNNTGG	1 cut(s)	1388
AccI	GTMKAC	1 cut(s)	1850
AccII	CGCG	2 cut(s)	1098, 1749
AccIII	TCCGGA	1 cut(s)	583
AciI	CCGC	5 cut(s)	395, 497, 554, 578, 1338
AclI	AACGTT	1 cut(s)	1728
AclWI	GGATC	5 cut(s)	117, 737, 750, 1186, 1445
AcoI	YGGCCR	2 cut(s)	546, 1025
AcsI	RAATTY	3 cut(s)	662, 1436, 1827
AcyI	GRCGYC	1 cut(s)	953
AdeI	CACNNNGTG	1 cut(s)	1650
AfaI	GTAC	5 cut(s)	542, 1017, 1031, 1112, 1863
AfiI	CCNNNNNNNGG	4 cut(s)	752, 1388, 1933, 1934
AflIII	ACRYGT	1 cut(s)	1096
AgeI	ACCGGT	1 cut(s)	1787
AgsI	TTSAA	5 cut(s)	54, 212, 472, 517, 1103
AhdI	GACNNNNNGTC	1 cut(s)	973
AjnI	CCWGG	5 cut(s)	160, 503, 1291, 1613, 1932
AloI	GAACNNNNNNTCC	2 cut(s)	803, 835
AluBI	AGCT	4 cut(s)	89, 520, 892, 1477
AluI	AGCT	4 cut(s)	89, 520, 892, 1477
Alw21I	GWGCWC	3 cut(s)	468, 862, 1922
Alw26I	GTCTC	4 cut(s)	1164, 1584, 1841, 1881
AlwI	GGATC	5 cut(s)	117, 737, 750, 1186, 1445
AlwNI	CAGNNNCTG	1 cut(s)	715
Aor13HI	TCCGGA	1 cut(s)	583
AoxI	GGCC	6 cut(s)	28, 479, 546, 829, 925, 1025
ApeKI	GCWGC	1 cut(s)	1430
ApoI	RAATTY	3 cut(s)	662, 1436, 1827
ArsI	GACNNNNNNTTYG	2 cut(s)	1583, 1615
AsiGI	ACCGGT	1 cut(s)	1787
Asp700I	GAANNNNTTC	1 cut(s)	534
AspLEI	GCGC	1 cut(s)	955
AspS9I	GGNCC	7 cut(s)	247, 797, 830, 877, 926, 1391, 1967
AsuC2I	CCSGG	2 cut(s)	1167, 1866
AsuHPI	GGTGA	4 cut(s)	310, 808, 1373, 1764
AsuII	TTCGAA	1 cut(s)	1705
AvaII	GGWCC	5 cut(s)	247, 797, 877, 1391, 1967
AxyI	CCTNAGG	1 cut(s)	1545
BalI	TGGCCA	1 cut(s)	548
BamHI	GGATCC	1 cut(s)	742
BanI	GGYRCC	2 cut(s)	952, 1159
Bbv12I	GWGCWC	3 cut(s)	468, 862, 1922
BbvI	GCAGC	1 cut(s)	1417
BccI	CCATC	6 cut(s)	464, 654, 1069, 1497, 1622, 1978
BceAI	ACGGC	2 cut(s)	148, 1012
BcgI	CGANNNNNNTGC	4 cut(s)	951, 985, 1262, 1296
BciT130I	CCWGG	5 cut(s)	162, 505, 1293, 1615, 1934
BcnI	CCSGG	2 cut(s)	1167, 1866
BcoDI	GTCTC	4 cut(s)	1164, 1584, 1841, 1881
BfaI	CTAG	2 cut(s)	1218, 1587
BfmI	CTRYAG	1 cut(s)	1739
BfoI	RGCGCY	1 cut(s)	956
BfuAI	ACCTGC	1 cut(s)	1331
BisI	GCNGC	2 cut(s)	578, 1431
BlsI	GCNGC	2 cut(s)	579, 1432
Bme1390I	CCNGG	7 cut(s)	162, 505, 1167, 1293, 1615, 1866, 1934
Bme18I	GGWCC	5 cut(s)	247, 797, 877, 1391, 1967
BmeRI	GACNNNNNGTC	1 cut(s)	973
BmgT120I	GGNCC	7 cut(s)	247, 797, 830, 877, 926, 1391, 1967
BmiI	GGNNCC	6 cut(s)	248, 374, 744, 954, 1161, 1290
BmrFI	CCNGG	7 cut(s)	162, 505, 1167, 1293, 1615, 1866, 1934
BmsI	GCATC	5 cut(s)	204, 949, 979, 1052, 1422
Bpu14I	TTCGAA	1 cut(s)	1705
BpuMI	CCSGG	2 cut(s)	1167, 1866
Bsa29I	ATCGAT	1 cut(s)	1312
BsaAI	YACGTR	4 cut(s)	1114, 1150, 1259, 1650
BsaBI	GATNNNNATC	1 cut(s)	1455
BsaHI	GRCGYC	1 cut(s)	953
BsaI	GGTCTC	1 cut(s)	1841
BsaJI	CCNNGG	3 cut(s)	908, 1382, 1933
BsaWI	WCCGGW	3 cut(s)	284, 583, 1787
BsaXI	ACNNNNNCTCC	2 cut(s)	803, 833
Bsc4I	CCNNNNNNNGG	4 cut(s)	752, 1388, 1933, 1934
Bse118I	RCCGGY	2 cut(s)	1787, 1842
Bse1I	ACTGG	4 cut(s)	181, 374, 1207, 1598
Bse21I	CCTNAGG	1 cut(s)	1545
Bse3DI	GCAATG	5 cut(s)	81, 784, 852, 1871, 1920
Bse8I	GATNNNNATC	1 cut(s)	1455
BseAI	TCCGGA	1 cut(s)	583
BseBI	CCWGG	5 cut(s)	162, 505, 1293, 1615, 1934
BseCI	ATCGAT	1 cut(s)	1312
BseDI	CCNNGG	3 cut(s)	908, 1382, 1933
BseGI	GGATG	7 cut(s)	592, 646, 964, 994, 1413, 1489, 1630
BseJI	GATNNNNATC	1 cut(s)	1455
BseLI	CCNNNNNNNGG	4 cut(s)	752, 1388, 1933, 1934
BseMI	GCAATG	5 cut(s)	81, 784, 852, 1871, 1920
BseMII	CTCAG	3 cut(s)	535, 868, 1536
BseNI	ACTGG	4 cut(s)	181, 374, 1207, 1598
BseRI	GAGGAG	1 cut(s)	1562
BseXI	GCAGC	1 cut(s)	1417
BseYI	CCCAGC	1 cut(s)	773
Bsh1236I	CGCG	2 cut(s)	1098, 1749
Bsh1285I	CGRYCG	1 cut(s)	1788
BshFI	GGCC	6 cut(s)	30, 481, 548, 831, 927, 1027
BshNI	GGYRCC	2 cut(s)	952, 1159
BshTI	ACCGGT	1 cut(s)	1787
BshVI	ATCGAT	1 cut(s)	1312
BsiEI	CGRYCG	1 cut(s)	1788
BsiHKAI	GWGCWC	3 cut(s)	468, 862, 1922
BsiSI	CCGG	7 cut(s)	285, 584, 956, 1166, 1788, 1843, 1866
BsiWI	CGTACG	1 cut(s)	1029
BslFI	GGGAC	5 cut(s)	67, 260, 377, 1430, 1950
BslI	CCNNNNNNNGG	4 cut(s)	752, 1388, 1933, 1934
BsmAI	GTCTC	4 cut(s)	1164, 1584, 1841, 1881
BsmBI	CGTCTC	1 cut(s)	1164
BsmFI	GGGAC	5 cut(s)	67, 260, 377, 1430, 1950
BsnI	GGCC	6 cut(s)	30, 481, 548, 831, 927, 1027
Bso31I	GGTCTC	1 cut(s)	1841
Bsp119I	TTCGAA	1 cut(s)	1705
Bsp1286I	GDGCHC	3 cut(s)	468, 862, 1922
Bsp13I	TCCGGA	1 cut(s)	583
Bsp19I	CCATGG	1 cut(s)	908
BspACI	CCGC	5 cut(s)	395, 497, 554, 578, 1338
BspANI	GGCC	6 cut(s)	30, 481, 548, 831, 927, 1027
BspCNI	CTCAG	3 cut(s)	534, 867, 1537
BspDI	ATCGAT	1 cut(s)	1312
BspEI	TCCGGA	1 cut(s)	583
BspFNI	CGCG	2 cut(s)	1098, 1749
BspHI	TCATGA	1 cut(s)	1048
BspLI	GGNNCC	6 cut(s)	248, 374, 744, 954, 1161, 1290
BspMI	ACCTGC	1 cut(s)	1331
BspPI	GGATC	5 cut(s)	117, 737, 750, 1186, 1445
BspT104I	TTCGAA	1 cut(s)	1705
BspT107I	GGYRCC	2 cut(s)	952, 1159
BspTNI	GGTCTC	1 cut(s)	1841
BsrDI	GCAATG	5 cut(s)	81, 784, 852, 1871, 1920
BsrFI	RCCGGY	2 cut(s)	1787, 1842
BsrI	ACTGG	4 cut(s)	181, 374, 1207, 1598
BssAI	RCCGGY	2 cut(s)	1787, 1842
BssECI	CCNNGG	3 cut(s)	908, 1382, 1933
BssNAI	GTATAC	1 cut(s)	1851
BssNI	GRCGYC	1 cut(s)	953
BssT1I	CCWWGG	2 cut(s)	908, 1382
Bst1107I	GTATAC	1 cut(s)	1851
Bst2UI	CCWGG	5 cut(s)	162, 505, 1293, 1615, 1934
Bst4CI	ACNGT	4 cut(s)	153, 322, 1142, 1567
BstACI	GRCGYC	1 cut(s)	953
BstAPI	GCANNNNNTGC	1 cut(s)	1730
BstBAI	YACGTR	4 cut(s)	1114, 1150, 1259, 1650
BstBI	TTCGAA	1 cut(s)	1705
BstDEI	CTNAG	4 cut(s)	521, 854, 1082, 1545
BstDSI	CCRYGG	1 cut(s)	908
BstF5I	GGATG	7 cut(s)	592, 646, 964, 994, 1413, 1489, 1630
BstFNI	CGCG	2 cut(s)	1098, 1749
BstH2I	RGCGCY	1 cut(s)	956
BstHHI	GCGC	1 cut(s)	955
BstMAI	GTCTC	4 cut(s)	1164, 1584, 1841, 1881
BstMCI	CGRYCG	1 cut(s)	1788
BstMWI	GCNNNNNNNGC	5 cut(s)	394, 959, 1095, 1142, 1730
BstNI	CCWGG	5 cut(s)	162, 505, 1293, 1615, 1934
BstNSI	RCATGY	2 cut(s)	392, 577
BstSCI	CCNGG	7 cut(s)	160, 503, 1165, 1291, 1613, 1864, 1932
BstSFI	CTRYAG	1 cut(s)	1739
BstSNI	TACGTA	1 cut(s)	1114
BstUI	CGCG	2 cut(s)	1098, 1749
BstV1I	GCAGC	1 cut(s)	1417
BstX2I	RGATCY	2 cut(s)	742, 1450
BstXI	CCANNNNNNTGG	1 cut(s)	1401
BstYI	RGATCY	2 cut(s)	742, 1450
BstZ17I	GTATAC	1 cut(s)	1851
Bsu15I	ATCGAT	1 cut(s)	1312
Bsu36I	CCTNAGG	1 cut(s)	1545
BsuRI	GGCC	6 cut(s)	30, 481, 548, 831, 927, 1027
BsuTUI	ATCGAT	1 cut(s)	1312
BtgI	CCRYGG	1 cut(s)	908
BtsCI	GGATG	7 cut(s)	592, 646, 964, 994, 1413, 1489, 1630
BtsIMutI	CAGTG	2 cut(s)	149, 862
BveI	ACCTGC	1 cut(s)	1331
CaiI	CAGNNNCTG	1 cut(s)	715
CciI	TCATGA	1 cut(s)	1048
CfoI	GCGC	1 cut(s)	955
Cfr10I	RCCGGY	2 cut(s)	1787, 1842
Cfr13I	GGNCC	7 cut(s)	247, 797, 830, 877, 926, 1391, 1967
ClaI	ATCGAT	1 cut(s)	1312
CseI	GACGC	1 cut(s)	1181
CsiI	ACCWGGT	1 cut(s)	160
Csp6I	GTAC	5 cut(s)	541, 1016, 1030, 1111, 1862
CspAI	ACCGGT	1 cut(s)	1787
CviQI	GTAC	5 cut(s)	541, 1016, 1030, 1111, 1862
DdeI	CTNAG	4 cut(s)	521, 854, 1082, 1545
DinI	GGCGCC	1 cut(s)	954
DraIII	CACNNNGTG	1 cut(s)	1650
DriI	GACNNNNNGTC	1 cut(s)	973
EaeI	YGGCCR	2 cut(s)	546, 1025
Eam1105I	GACNNNNNGTC	1 cut(s)	973
Eco105I	TACGTA	1 cut(s)	1114
Eco130I	CCWWGG	2 cut(s)	908, 1382
Eco31I	GGTCTC	1 cut(s)	1841
Eco32I	GATATC	1 cut(s)	354
Eco47I	GGWCC	5 cut(s)	247, 797, 877, 1391, 1967
Eco81I	CCTNAGG	1 cut(s)	1545
EcoO109I	RGGNCCY	2 cut(s)	247, 877
EcoRI	GAATTC	1 cut(s)	1827
EcoRII	CCWGG	5 cut(s)	160, 503, 1291, 1613, 1932
EcoRV	GATATC	1 cut(s)	354
EcoT14I	CCWWGG	2 cut(s)	908, 1382
EcoT22I	ATGCAT	2 cut(s)	740, 1285
EgeI	GGCGCC	1 cut(s)	954
EheI	GGCGCC	1 cut(s)	954
ErhI	CCWWGG	2 cut(s)	908, 1382
Esp3I	CGTCTC	1 cut(s)	1164
FalI	AAGNNNNNCTT	2 cut(s)	1108, 1140
FaqI	GGGAC	5 cut(s)	67, 260, 377, 1430, 1950
FauI	CCCGC	1 cut(s)	402
FblI	GTMKAC	1 cut(s)	1850
Fnu4HI	GCNGC	2 cut(s)	578, 1431
FokI	GGATG	7 cut(s)	599, 633, 971, 1001, 1400, 1476, 1637
Fsp4HI	GCNGC	2 cut(s)	578, 1431
FspBI	CTAG	2 cut(s)	1218, 1587
GlaI	GCGC	1 cut(s)	954
GluI	GCNGC	2 cut(s)	578, 1431
GsaI	CCCAGC	1 cut(s)	777
HaeII	RGCGCY	1 cut(s)	956
HaeIII	GGCC	6 cut(s)	30, 481, 548, 831, 927, 1027
HapII	CCGG	7 cut(s)	285, 584, 956, 1166, 1788, 1843, 1866
HgaI	GACGC	1 cut(s)	1181
HhaI	GCGC	1 cut(s)	955
Hin1I	GRCGYC	1 cut(s)	953
Hin6I	GCGC	1 cut(s)	953
HinP1I	GCGC	1 cut(s)	953
HincII	GTYRAC	1 cut(s)	1486
HindII	GTYRAC	1 cut(s)	1486
HinfI	GANTC	7 cut(s)	60, 712, 968, 1744, 1757, 1769, 1938
HpaII	CCGG	7 cut(s)	285, 584, 956, 1166, 1788, 1843, 1866
HphI	GGTGA	4 cut(s)	310, 808, 1373, 1764
Hpy166II	GTNNAC	6 cut(s)	318, 797, 1391, 1486, 1563, 1851
Hpy188I	TCNGA	7 cut(s)	219, 278, 788, 1066, 1230, 1686, 1799
Hpy8I	GTNNAC	6 cut(s)	318, 797, 1391, 1486, 1563, 1851
Hpy99I	CGWCG	1 cut(s)	755
HpyAV	CCTTC	5 cut(s)	503, 910, 1615, 1877, 1958
HpyCH4III	ACNGT	4 cut(s)	153, 322, 1142, 1567
HpyCH4IV	ACGT	8 cut(s)	1113, 1149, 1258, 1332, 1608, 1649, 1728, 1860
HpyF10VI	GCNNNNNNNGC	5 cut(s)	394, 959, 1095, 1142, 1730
HpyF3I	CTNAG	4 cut(s)	521, 854, 1082, 1545
HpySE526I	ACGT	8 cut(s)	1113, 1149, 1258, 1332, 1608, 1649, 1728, 1860
Hsp92I	GRCGYC	1 cut(s)	953
HspAI	GCGC	1 cut(s)	953
KasI	GGCGCC	1 cut(s)	952
Kpn2I	TCCGGA	1 cut(s)	583
Lsp1109I	GCAGC	1 cut(s)	1417
LweI	GCATC	5 cut(s)	204, 949, 979, 1052, 1422
MabI	ACCWGGT	1 cut(s)	160
MaeI	CTAG	2 cut(s)	1218, 1587
MaeII	ACGT	8 cut(s)	1113, 1149, 1258, 1332, 1608, 1649, 1728, 1860
MaeIII	GTNAC	5 cut(s)	145, 172, 975, 1333, 1609
MboII	GAAGA	3 cut(s)	629, 925, 1630
MfeI	CAATTG	2 cut(s)	69, 95
MflI	RGATCY	2 cut(s)	742, 1450
MhlI	GDGCHC	3 cut(s)	468, 862, 1922
MlsI	TGGCCA	1 cut(s)	548
MluI	ACGCGT	1 cut(s)	1096
MluNI	TGGCCA	1 cut(s)	548
Mly113I	GGCGCC	1 cut(s)	953
MlyI	GAGTC	3 cut(s)	69, 962, 1932
MmeI	TCCRAC	2 cut(s)	256, 710
Mox20I	TGGCCA	1 cut(s)	548
Mph1103I	ATGCAT	2 cut(s)	740, 1285
MroI	TCCGGA	1 cut(s)	583
MroXI	GAANNNNTTC	1 cut(s)	534
MscI	TGGCCA	1 cut(s)	548
MseI	TTAA	5 cut(s)	303, 489, 804, 945, 1329
Msp20I	TGGCCA	1 cut(s)	548
MspI	CCGG	7 cut(s)	285, 584, 956, 1166, 1788, 1843, 1866
MspR9I	CCNGG	7 cut(s)	162, 505, 1167, 1293, 1615, 1866, 1934
MunI	CAATTG	2 cut(s)	69, 95
MvaI	CCWGG	5 cut(s)	162, 505, 1293, 1615, 1934
MvnI	CGCG	2 cut(s)	1098, 1749
MwoI	GCNNNNNNNGC	5 cut(s)	394, 959, 1095, 1142, 1730
NarI	GGCGCC	1 cut(s)	953
NciI	CCSGG	2 cut(s)	1167, 1866
NcoI	CCATGG	1 cut(s)	908
NlaIV	GGNNCC	6 cut(s)	248, 374, 744, 954, 1161, 1290
NmuCI	GTSAC	1 cut(s)	975
NsiI	ATGCAT	2 cut(s)	740, 1285
NspI	RCATGY	2 cut(s)	392, 577
NspV	TTCGAA	1 cut(s)	1705
PagI	TCATGA	1 cut(s)	1048
PcsI	WCGNNNNNNNCGW	1 cut(s)	1146
PdmI	GAANNNNTTC	1 cut(s)	534
PfeI	GAWTC	4 cut(s)	712, 1744, 1757, 1769
Pfl23II	CGTACG	1 cut(s)	1029
PflFI	GACNNNGTC	1 cut(s)	1676
PflMI	CCANNNNNTGG	1 cut(s)	1388
PfoI	TCCNGGA	1 cut(s)	1165
PinAI	ACCGGT	1 cut(s)	1787
PkrI	GCNGC	2 cut(s)	579, 1432
PleI	GAGTC	3 cut(s)	68, 962, 1932
PluTI	GGCGCC	1 cut(s)	956
PpsI	GAGTC	3 cut(s)	68, 962, 1932
Ppu21I	YACGTR	4 cut(s)	1114, 1150, 1259, 1650
PpuMI	RGGWCCY	2 cut(s)	247, 877
PsiI	TTATAA	2 cut(s)	122, 419
Psp1406I	AACGTT	1 cut(s)	1728
Psp5II	RGGWCCY	2 cut(s)	247, 877
Psp6I	CCWGG	5 cut(s)	160, 503, 1291, 1613, 1932
PspFI	CCCAGC	1 cut(s)	773
PspGI	CCWGG	5 cut(s)	160, 503, 1291, 1613, 1932
PspLI	CGTACG	1 cut(s)	1029
PspN4I	GGNNCC	6 cut(s)	248, 374, 744, 954, 1161, 1290
PspPI	GGNCC	7 cut(s)	247, 797, 830, 877, 926, 1391, 1967
PspPPI	RGGWCCY	2 cut(s)	247, 877
PstNI	CAGNNNCTG	1 cut(s)	715
PsuI	RGATCY	2 cut(s)	742, 1450
PsyI	GACNNNGTC	1 cut(s)	1676
RsaI	GTAC	5 cut(s)	542, 1017, 1031, 1112, 1863
RsaNI	GTAC	5 cut(s)	541, 1016, 1030, 1111, 1862
SaqAI	TTAA	5 cut(s)	303, 489, 804, 945, 1329
SatI	GCNGC	2 cut(s)	578, 1431
Sau96I	GGNCC	7 cut(s)	247, 797, 830, 877, 926, 1391, 1967
SchI	GAGTC	3 cut(s)	69, 962, 1932
ScrFI	CCNGG	7 cut(s)	162, 505, 1167, 1293, 1615, 1866, 1934
SduI	GDGCHC	3 cut(s)	468, 862, 1922
SexAI	ACCWGGT	1 cut(s)	160
SfaNI	GCATC	5 cut(s)	204, 949, 979, 1052, 1422
SfcI	CTRYAG	1 cut(s)	1739
SfoI	GGCGCC	1 cut(s)	954
SfuI	TTCGAA	1 cut(s)	1705
SinI	GGWCC	5 cut(s)	247, 797, 877, 1391, 1967
SnaBI	TACGTA	1 cut(s)	1114
SsiI	CCGC	5 cut(s)	395, 497, 554, 578, 1338
SspDI	GGCGCC	1 cut(s)	952
SspMI	CTAG	2 cut(s)	1218, 1587
StyD4I	CCNGG	7 cut(s)	160, 503, 1165, 1291, 1613, 1864, 1932
StyI	CCWWGG	2 cut(s)	908, 1382
TaaI	ACNGT	4 cut(s)	153, 322, 1142, 1567
TaiI	ACGT	8 cut(s)	1116, 1152, 1261, 1335, 1611, 1652, 1731, 1863
TaqI	TCGA	4 cut(s)	678, 1312, 1666, 1705
TaqII	GACCGA	1 cut(s)	606
TatI	WGTACW	1 cut(s)	1015
TauI	GCSGC	1 cut(s)	580
TfiI	GAWTC	4 cut(s)	712, 1744, 1757, 1769
Tru1I	TTAA	5 cut(s)	303, 489, 804, 945, 1329
Tru9I	TTAA	5 cut(s)	303, 489, 804, 945, 1329
TscAI	CASTG	2 cut(s)	156, 862
TseFI	GTSAC	1 cut(s)	975
TseI	GCWGC	1 cut(s)	1430
Tsp45I	GTSAC	1 cut(s)	975
TspDTI	ATGAA	3 cut(s)	1335, 1389, 1820
TspGWI	ACGGA	1 cut(s)	1946
TspRI	CASTG	2 cut(s)	156, 862
Tth111I	GACNNNGTC	1 cut(s)	1676
Van91I	CCANNNNNTGG	1 cut(s)	1388
VpaK11BI	GGWCC	5 cut(s)	247, 797, 877, 1391, 1967
XapI	RAATTY	3 cut(s)	662, 1436, 1827
XceI	RCATGY	2 cut(s)	392, 577
XcmI	CCANNNNNNNNNTGG	1 cut(s)	906
XmiI	GTMKAC	1 cut(s)	1850
XmnI	GAANNNNTTC	1 cut(s)	534
XspI	CTAG	2 cut(s)	1218, 1587
Zsp2I	ATGCAT	2 cut(s)	740, 1285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.