Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002285

Physical Location & Seq

Genomic Coordinates Reverse (-)

10910525 .. 10927527

Gene Structure 17003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00227030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGTCGTCCCGAGCTGTGCAGTTGCATATTCAGGATCATCATGTTATAATGGATAACGGCATACTCCAAGTTACACTATCAAAACCAGGTGGAATTGTAACTGGAATACAATATAATGGCATCAACAATTTGCTTGAAGTTCAGAATACAGAAACTAATAGAGGGTATTGGGACCTTGTTTGGAGTGAAGCAGGAAGTGTCGGAACAACCGGAACATTTGATGTAATTAAAGGAACAAAGTTCACCGTCATAGTGGAAAACGAGGAACAGGTAGAGATATCATTCACAAGGAACTGGAACCATAAGAACAAGCATGTCCCGCTAAATATAGACAAAAGGTTTATAATGCTTCGTAATTCGTCAGGCTTCTACTCGTATGCCATCTATGAGCACTTGAAAGAATGGCCTCCTTTTAACCTTCCCCAGACCAGGATAACATTCAAGCTCAGTCAAGAAAAGTTTCGGTACATGGCCATAGCGGATAACAGGCAAAGATACATGCCGCTTCCGGATGACCGATTACCAGACAGAAGCAAAGTTTTAGATTTTCCAGAGGCAGTTCTTCTTGTCCATCCCATAGAGCCAGAATTTGAGGGAGAGGTCGATGACAAATATCAATACTCCTGCGAGAACCAGAATCTGCAAATGCATGGTTGGATATGCATGGATCCACCCGTCGGGTTCTGGCAAATAACACCCAGCAATGAGTTCCGATCTGGTGGACCTCTTAAACAGAACCTCACCTCCCATGTTGGCCCAACTTGTCTTGCAATGTTTCTCAGTGCTCACTATTCAGGAGAGGACCTTGTGCTAAAGCTCAAACCAGATGAGCCATGGAAGAAGGTGTTTGGCCCTATTTTTATCTATCTTAATTCGGCGCCGGATGCAAAAGACTCGTTGTCACTCTGGGAGGATGCAAAGGCACAGATGATGACAGAAGTACAAAGTTGGCCGTACGAGTTTCCAGCATCTCATGACTTTCCACCATCAGATCAACGGGGTAATCTTAGTGGCAGAATACGCGTTCAAGATAGGTACGTAAGCGATACTTATATACTCGCCAACGGTGCTTATGTGGGCTTGGCACCTCCGGGAGACGCTGGATCATGGCAGAGAGATTGCAAGGGCTACCAGTTCTGGACTAGGGCAGATCAGAATGGTTATTACTCTATAAATGATATACGTGAGGGCAACTACGATCTTTATGCATGGGTTCCTGGTTTTATAGGAGATTATCGATATGATGAAACCATTAACGTAACCGCAGGTTGTGATATTGACATTGGTGATATTGTATATGAACCACCAAGGAGTGGACCAACATTGTGGGAAATTGGCATCCCTGATCGTTCTGCTGCTGAATTTTATGTCCCAGATCCTAATCCAAAGTATATCAATAAGCTATATGTCAACCATCCTGACAGGTTTAGGCAGTATGGTTTATGGGAAAGATATGCAGACTTATATCCTGAGGAGGATTTAGTGTTTACAGTTGGTGTTAGTGATTATACTAGAGACTGGTTCTTTGCTCAATTTACCAGGAAGAAGGATGATGGCAAGTATCAAGGCACTACGTGGCAAATAAAGTTTAGACTTGACAATGTCAGTCGGACCAAAACATACACACTTCGAATTGCCCTTGCTACTGCAAACGTTGCAGAACTACAGATTCGCGTAAATGATTCCAAGACAGATTCACCTGTATTTACGACCGGTGTGATCGGACACGACAACACAATTTGCAGACATGGAATTCATGGTCTCTACCGGCTGTATACAATAGACGTACCCGGCACATTGCTTGTGGAAGGAGACAACATAGTGTTTCTAACCCAAGCAATGAGCACCAGTCCTTTCCAGGGACTCATGTATGACTATATCCGTTTAGAAGGTCCATCAACTTCTATTTCTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

638

Amino Acids

73.15

Weight (kDa)

5.25

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhamnogal_lyase	PF06045	4 - 201	1.7e-90	Rhamnogalacturonate lyase family
fn3_3	PF14686	355 - 427	5.4e-27	Polysaccharide lyase family 4, domain II
CBM-like	PF14683	441 - 629	3.1e-53	Polysaccharide lyase family 4, domain III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24430 AT4G24430
fragaria_vesca	FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34131 FvH4_5g34150
malus_domestica	MD08G1204400.v1.1 MD08G1205000.v1.1
prunus_persica	Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536600_v2.0.a1 Prupe.1G536700_v2.0.a1 Prupe.1G536700_v2.0.a1
pyrus_communis	pycom08g17670 pycom08g17720
rosa_chinensis	RchiOBHm_Chr7g0233711 RchiOBHm_Chr7g0233721 RchiOBHm_Chr7g0233731 RchiOBHm_Chr7g0233741
rosa_laevigata	RLG00000001237 RLG00000001239 RLG00000001240
rosa_multiflora	Rmu_sc0000740.1_g000047 Rmu_sc0000740.1_g000058 Rmu_sc0010145.1_g000001 Rmu_sc0011583.1_g000004
rosa_roxburghii	Rroxscaffold_3G00227030 Rroxscaffold_3G00227050 Rroxscaffold_3G00227070
rosa_rugosa	Rorug07G0282300 Rorug07G0282300
rosa_samantha	Rh7AG438600 Rh7AG438700 Rh7BG411300 Rh7CG458200 Rh7CG458300 Rh7CG458400 Rh7DG428200
rosa_wichuraiana	Rw7G036170 Rw7G036180 Rw7G036230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	47, 344
Acc36I	ACCTGC	1 cut(s)	1256
AccB1I	GGYRCC	2 cut(s)	877, 1084
AccB7I	CCANNNNNTGG	1 cut(s)	1313
AccI	GTMKAC	1 cut(s)	1775
AccII	CGCG	2 cut(s)	1023, 1674
AccIII	TCCGGA	1 cut(s)	508
AciI	CCGC	4 cut(s)	320, 479, 503, 1263
AclI	AACGTT	1 cut(s)	1653
AclWI	GGATC	5 cut(s)	42, 662, 675, 1111, 1370
AcoI	YGGCCR	2 cut(s)	471, 950
AcsI	RAATTY	3 cut(s)	587, 1361, 1752
AcyI	GRCGYC	1 cut(s)	878
AdeI	CACNNNGTG	1 cut(s)	1575
AfaI	GTAC	5 cut(s)	467, 942, 956, 1037, 1788
AfiI	CCNNNNNNNGG	4 cut(s)	429, 677, 1313, 1859
AflIII	ACRYGT	1 cut(s)	1021
AgeI	ACCGGT	1 cut(s)	1712
AgsI	TTSAA	4 cut(s)	137, 397, 442, 1028
AhdI	GACNNNNNGTC	1 cut(s)	898
AjnI	CCWGG	5 cut(s)	85, 428, 1216, 1538, 1857
AloI	GAACNNNNNNTCC	2 cut(s)	728, 760
AluBI	AGCT	4 cut(s)	14, 445, 817, 1402
AluI	AGCT	4 cut(s)	14, 445, 817, 1402
Alw21I	GWGCWC	3 cut(s)	393, 787, 1847
Alw26I	GTCTC	4 cut(s)	1089, 1509, 1766, 1806
AlwI	GGATC	5 cut(s)	42, 662, 675, 1111, 1370
AlwNI	CAGNNNCTG	1 cut(s)	640
Ama87I	CYCGRG	1 cut(s)	9
Aor13HI	TCCGGA	1 cut(s)	508
AoxI	GGCC	5 cut(s)	404, 471, 754, 850, 950
ApeKI	GCWGC	1 cut(s)	1355
ApoI	RAATTY	3 cut(s)	587, 1361, 1752
ArsI	GACNNNNNNTTYG	2 cut(s)	1508, 1540
AsiGI	ACCGGT	1 cut(s)	1712
Asp700I	GAANNNNTTC	1 cut(s)	459
AspLEI	GCGC	1 cut(s)	880
AspS9I	GGNCC	8 cut(s)	172, 722, 755, 802, 851, 1316, 1611, 1892
AsuC2I	CCSGG	2 cut(s)	1092, 1791
AsuHPI	GGTGA	4 cut(s)	235, 733, 1298, 1689
AsuII	TTCGAA	1 cut(s)	1630
AvaI	CYCGRG	1 cut(s)	9
AvaII	GGWCC	6 cut(s)	172, 722, 802, 1316, 1611, 1892
AxyI	CCTNAGG	1 cut(s)	1470
BalI	TGGCCA	1 cut(s)	473
BamHI	GGATCC	1 cut(s)	667
BanI	GGYRCC	2 cut(s)	877, 1084
Bbv12I	GWGCWC	3 cut(s)	393, 787, 1847
BbvI	GCAGC	1 cut(s)	1342
BccI	CCATC	6 cut(s)	389, 579, 994, 1422, 1547, 1903
BceAI	ACGGC	2 cut(s)	73, 937
BcgI	CGANNNNNNTGC	4 cut(s)	876, 910, 1187, 1221
BciT130I	CCWGG	5 cut(s)	87, 430, 1218, 1540, 1859
BcnI	CCSGG	2 cut(s)	1092, 1791
BcoDI	GTCTC	4 cut(s)	1089, 1509, 1766, 1806
BfaI	CTAG	2 cut(s)	1143, 1512
BfmI	CTRYAG	1 cut(s)	1664
BfoI	RGCGCY	1 cut(s)	881
BfuAI	ACCTGC	1 cut(s)	1256
BisI	GCNGC	2 cut(s)	503, 1356
BlsI	GCNGC	2 cut(s)	504, 1357
Bme1390I	CCNGG	7 cut(s)	87, 430, 1092, 1218, 1540, 1791, 1859
Bme18I	GGWCC	6 cut(s)	172, 722, 802, 1316, 1611, 1892
BmeRI	GACNNNNNGTC	1 cut(s)	898
BmeT110I	CYCGRG	1 cut(s)	9
BmgT120I	GGNCC	8 cut(s)	172, 722, 755, 802, 851, 1316, 1611, 1892
BmiI	GGNNCC	6 cut(s)	173, 299, 669, 879, 1086, 1215
BmrFI	CCNGG	7 cut(s)	87, 430, 1092, 1218, 1540, 1791, 1859
BmsI	GCATC	5 cut(s)	129, 874, 904, 977, 1347
Bpu14I	TTCGAA	1 cut(s)	1630
BpuMI	CCSGG	2 cut(s)	1092, 1791
Bsa29I	ATCGAT	1 cut(s)	1237
BsaAI	YACGTR	3 cut(s)	1039, 1184, 1575
BsaBI	GATNNNNATC	1 cut(s)	1380
BsaHI	GRCGYC	1 cut(s)	878
BsaI	GGTCTC	1 cut(s)	1766
BsaJI	CCNNGG	3 cut(s)	833, 1307, 1858
BsaWI	WCCGGW	3 cut(s)	209, 508, 1712
BsaXI	ACNNNNNCTCC	2 cut(s)	728, 758
Bsc4I	CCNNNNNNNGG	4 cut(s)	429, 677, 1313, 1859
Bse118I	RCCGGY	2 cut(s)	1712, 1767
Bse1I	ACTGG	5 cut(s)	106, 299, 1132, 1523, 1848
Bse21I	CCTNAGG	1 cut(s)	1470
Bse3DI	GCAATG	4 cut(s)	709, 777, 1796, 1845
Bse8I	GATNNNNATC	1 cut(s)	1380
BseAI	TCCGGA	1 cut(s)	508
BseBI	CCWGG	5 cut(s)	87, 430, 1218, 1540, 1859
BseCI	ATCGAT	1 cut(s)	1237
BseDI	CCNNGG	3 cut(s)	833, 1307, 1858
BseGI	GGATG	7 cut(s)	517, 571, 889, 919, 1338, 1414, 1555
BseJI	GATNNNNATC	1 cut(s)	1380
BseLI	CCNNNNNNNGG	4 cut(s)	429, 677, 1313, 1859
BseMI	GCAATG	4 cut(s)	709, 777, 1796, 1845
BseMII	CTCAG	3 cut(s)	460, 793, 1461
BseNI	ACTGG	5 cut(s)	106, 299, 1132, 1523, 1848
BseRI	GAGGAG	1 cut(s)	1487
BseXI	GCAGC	1 cut(s)	1342
BseYI	CCCAGC	1 cut(s)	698
BsgI	GTGCAG	1 cut(s)	38
Bsh1236I	CGCG	2 cut(s)	1023, 1674
Bsh1285I	CGRYCG	1 cut(s)	1713
BshFI	GGCC	5 cut(s)	406, 473, 756, 852, 952
BshNI	GGYRCC	2 cut(s)	877, 1084
BshTI	ACCGGT	1 cut(s)	1712
BshVI	ATCGAT	1 cut(s)	1237
BsiEI	CGRYCG	1 cut(s)	1713
BsiHKAI	GWGCWC	3 cut(s)	393, 787, 1847
BsiHKCI	CYCGRG	1 cut(s)	9
BsiSI	CCGG	7 cut(s)	210, 509, 881, 1091, 1713, 1768, 1791
BsiWI	CGTACG	1 cut(s)	954
BslFI	GGGAC	4 cut(s)	185, 302, 1355, 1875
BslI	CCNNNNNNNGG	4 cut(s)	429, 677, 1313, 1859
BsmAI	GTCTC	4 cut(s)	1089, 1509, 1766, 1806
BsmBI	CGTCTC	1 cut(s)	1089
BsmFI	GGGAC	4 cut(s)	185, 302, 1355, 1875
BsnI	GGCC	5 cut(s)	406, 473, 756, 852, 952
Bso31I	GGTCTC	1 cut(s)	1766
BsoBI	CYCGRG	1 cut(s)	9
Bsp119I	TTCGAA	1 cut(s)	1630
Bsp1286I	GDGCHC	3 cut(s)	393, 787, 1847
Bsp13I	TCCGGA	1 cut(s)	508
Bsp19I	CCATGG	1 cut(s)	833
BspACI	CCGC	4 cut(s)	320, 479, 503, 1263
BspANI	GGCC	5 cut(s)	406, 473, 756, 852, 952
BspCNI	CTCAG	3 cut(s)	459, 792, 1462
BspDI	ATCGAT	1 cut(s)	1237
BspEI	TCCGGA	1 cut(s)	508
BspFNI	CGCG	2 cut(s)	1023, 1674
BspHI	TCATGA	1 cut(s)	973
BspLI	GGNNCC	6 cut(s)	173, 299, 669, 879, 1086, 1215
BspMI	ACCTGC	1 cut(s)	1256
BspPI	GGATC	5 cut(s)	42, 662, 675, 1111, 1370
BspT104I	TTCGAA	1 cut(s)	1630
BspT107I	GGYRCC	2 cut(s)	877, 1084
BspTNI	GGTCTC	1 cut(s)	1766
BsrDI	GCAATG	4 cut(s)	709, 777, 1796, 1845
BsrFI	RCCGGY	2 cut(s)	1712, 1767
BsrI	ACTGG	5 cut(s)	106, 299, 1132, 1523, 1848
BssAI	RCCGGY	2 cut(s)	1712, 1767
BssECI	CCNNGG	3 cut(s)	833, 1307, 1858
BssNAI	GTATAC	1 cut(s)	1776
BssNI	GRCGYC	1 cut(s)	878
BssT1I	CCWWGG	2 cut(s)	833, 1307
Bst1107I	GTATAC	1 cut(s)	1776
Bst2UI	CCWGG	5 cut(s)	87, 430, 1218, 1540, 1859
Bst4CI	ACNGT	3 cut(s)	247, 1067, 1492
BstACI	GRCGYC	1 cut(s)	878
BstAPI	GCANNNNNTGC	1 cut(s)	1655
BstBAI	YACGTR	3 cut(s)	1039, 1184, 1575
BstBI	TTCGAA	1 cut(s)	1630
BstDEI	CTNAG	4 cut(s)	446, 779, 1007, 1470
BstDSI	CCRYGG	1 cut(s)	833
BstF5I	GGATG	7 cut(s)	517, 571, 889, 919, 1338, 1414, 1555
BstFNI	CGCG	2 cut(s)	1023, 1674
BstH2I	RGCGCY	1 cut(s)	881
BstHHI	GCGC	1 cut(s)	880
BstMAI	GTCTC	4 cut(s)	1089, 1509, 1766, 1806
BstMCI	CGRYCG	1 cut(s)	1713
BstMWI	GCNNNNNNNGC	5 cut(s)	319, 884, 1020, 1067, 1655
BstNI	CCWGG	5 cut(s)	87, 430, 1218, 1540, 1859
BstNSI	RCATGY	2 cut(s)	317, 502
BstSCI	CCNGG	7 cut(s)	85, 428, 1090, 1216, 1538, 1789, 1857
BstSFI	CTRYAG	1 cut(s)	1664
BstSNI	TACGTA	1 cut(s)	1039
BstUI	CGCG	2 cut(s)	1023, 1674
BstV1I	GCAGC	1 cut(s)	1342
BstX2I	RGATCY	2 cut(s)	667, 1375
BstXI	CCANNNNNNTGG	1 cut(s)	1326
BstYI	RGATCY	2 cut(s)	667, 1375
BstZ17I	GTATAC	1 cut(s)	1776
Bsu15I	ATCGAT	1 cut(s)	1237
Bsu36I	CCTNAGG	1 cut(s)	1470
BsuRI	GGCC	5 cut(s)	406, 473, 756, 852, 952
BsuTUI	ATCGAT	1 cut(s)	1237
BtgI	CCRYGG	1 cut(s)	833
BtsCI	GGATG	7 cut(s)	517, 571, 889, 919, 1338, 1414, 1555
BtsIMutI	CAGTG	1 cut(s)	787
BveI	ACCTGC	1 cut(s)	1256
CaiI	CAGNNNCTG	1 cut(s)	640
CciI	TCATGA	1 cut(s)	973
CfoI	GCGC	1 cut(s)	880
Cfr10I	RCCGGY	2 cut(s)	1712, 1767
Cfr13I	GGNCC	8 cut(s)	172, 722, 755, 802, 851, 1316, 1611, 1892
ClaI	ATCGAT	1 cut(s)	1237
CseI	GACGC	1 cut(s)	1106
CsiI	ACCWGGT	1 cut(s)	85
Csp6I	GTAC	5 cut(s)	466, 941, 955, 1036, 1787
CspAI	ACCGGT	1 cut(s)	1712
CviQI	GTAC	5 cut(s)	466, 941, 955, 1036, 1787
DdeI	CTNAG	4 cut(s)	446, 779, 1007, 1470
DinI	GGCGCC	1 cut(s)	879
DraIII	CACNNNGTG	1 cut(s)	1575
DriI	GACNNNNNGTC	1 cut(s)	898
EaeI	YGGCCR	2 cut(s)	471, 950
Eam1105I	GACNNNNNGTC	1 cut(s)	898
Eco105I	TACGTA	1 cut(s)	1039
Eco130I	CCWWGG	2 cut(s)	833, 1307
Eco31I	GGTCTC	1 cut(s)	1766
Eco32I	GATATC	1 cut(s)	279
Eco47I	GGWCC	6 cut(s)	172, 722, 802, 1316, 1611, 1892
Eco81I	CCTNAGG	1 cut(s)	1470
Eco88I	CYCGRG	1 cut(s)	9
EcoO109I	RGGNCCY	2 cut(s)	172, 802
EcoRI	GAATTC	1 cut(s)	1752
EcoRII	CCWGG	5 cut(s)	85, 428, 1216, 1538, 1857
EcoRV	GATATC	1 cut(s)	279
EcoT14I	CCWWGG	2 cut(s)	833, 1307
EcoT22I	ATGCAT	3 cut(s)	651, 665, 1210
EgeI	GGCGCC	1 cut(s)	879
EheI	GGCGCC	1 cut(s)	879
ErhI	CCWWGG	2 cut(s)	833, 1307
Esp3I	CGTCTC	1 cut(s)	1089
FalI	AAGNNNNNCTT	2 cut(s)	1033, 1065
FaqI	GGGAC	4 cut(s)	185, 302, 1355, 1875
FauI	CCCGC	1 cut(s)	327
FblI	GTMKAC	1 cut(s)	1775
Fnu4HI	GCNGC	2 cut(s)	503, 1356
FokI	GGATG	7 cut(s)	524, 558, 896, 926, 1325, 1401, 1562
Fsp4HI	GCNGC	2 cut(s)	503, 1356
FspBI	CTAG	2 cut(s)	1143, 1512
GlaI	GCGC	1 cut(s)	879
GluI	GCNGC	2 cut(s)	503, 1356
GsaI	CCCAGC	1 cut(s)	702
HaeII	RGCGCY	1 cut(s)	881
HaeIII	GGCC	5 cut(s)	406, 473, 756, 852, 952
HapII	CCGG	7 cut(s)	210, 509, 881, 1091, 1713, 1768, 1791
HgaI	GACGC	1 cut(s)	1106
HhaI	GCGC	1 cut(s)	880
Hin1I	GRCGYC	1 cut(s)	878
Hin6I	GCGC	1 cut(s)	878
HinP1I	GCGC	1 cut(s)	878
HincII	GTYRAC	1 cut(s)	1411
HindII	GTYRAC	1 cut(s)	1411
HinfI	GANTC	6 cut(s)	637, 893, 1669, 1682, 1694, 1863
HpaII	CCGG	7 cut(s)	210, 509, 881, 1091, 1713, 1768, 1791
HphI	GGTGA	4 cut(s)	235, 733, 1298, 1689
Hpy166II	GTNNAC	6 cut(s)	243, 722, 1316, 1411, 1488, 1776
Hpy188I	TCNGA	7 cut(s)	144, 203, 713, 991, 1155, 1611, 1724
Hpy8I	GTNNAC	6 cut(s)	243, 722, 1316, 1411, 1488, 1776
Hpy99I	CGWCG	1 cut(s)	680
HpyAV	CCTTC	5 cut(s)	428, 835, 1540, 1802, 1883
HpyCH4III	ACNGT	3 cut(s)	247, 1067, 1492
HpyCH4IV	ACGT	6 cut(s)	1038, 1183, 1257, 1574, 1653, 1785
HpyF10VI	GCNNNNNNNGC	5 cut(s)	319, 884, 1020, 1067, 1655
HpyF3I	CTNAG	4 cut(s)	446, 779, 1007, 1470
HpySE526I	ACGT	6 cut(s)	1038, 1183, 1257, 1574, 1653, 1785
Hsp92I	GRCGYC	1 cut(s)	878
HspAI	GCGC	1 cut(s)	878
KasI	GGCGCC	1 cut(s)	877
Kpn2I	TCCGGA	1 cut(s)	508
Lsp1109I	GCAGC	1 cut(s)	1342
LweI	GCATC	5 cut(s)	129, 874, 904, 977, 1347
MabI	ACCWGGT	1 cut(s)	85
MaeI	CTAG	2 cut(s)	1143, 1512
MaeII	ACGT	6 cut(s)	1038, 1183, 1257, 1574, 1653, 1785
MaeIII	GTNAC	4 cut(s)	70, 97, 900, 1258
MboII	GAAGA	3 cut(s)	554, 850, 1555
MflI	RGATCY	2 cut(s)	667, 1375
MhlI	GDGCHC	3 cut(s)	393, 787, 1847
MlsI	TGGCCA	1 cut(s)	473
MluI	ACGCGT	1 cut(s)	1021
MluNI	TGGCCA	1 cut(s)	473
Mly113I	GGCGCC	1 cut(s)	878
MlyI	GAGTC	2 cut(s)	887, 1857
MmeI	TCCRAC	3 cut(s)	181, 635, 1589
Mox20I	TGGCCA	1 cut(s)	473
Mph1103I	ATGCAT	3 cut(s)	651, 665, 1210
MroI	TCCGGA	1 cut(s)	508
MroXI	GAANNNNTTC	1 cut(s)	459
MscI	TGGCCA	1 cut(s)	473
MseI	TTAA	5 cut(s)	228, 414, 729, 870, 1254
Msp20I	TGGCCA	1 cut(s)	473
MspI	CCGG	7 cut(s)	210, 509, 881, 1091, 1713, 1768, 1791
MspR9I	CCNGG	7 cut(s)	87, 430, 1092, 1218, 1540, 1791, 1859
MvaI	CCWGG	5 cut(s)	87, 430, 1218, 1540, 1859
MvnI	CGCG	2 cut(s)	1023, 1674
MwoI	GCNNNNNNNGC	5 cut(s)	319, 884, 1020, 1067, 1655
NarI	GGCGCC	1 cut(s)	878
NciI	CCSGG	2 cut(s)	1092, 1791
NcoI	CCATGG	1 cut(s)	833
NlaIV	GGNNCC	6 cut(s)	173, 299, 669, 879, 1086, 1215
NmuCI	GTSAC	1 cut(s)	900
NsiI	ATGCAT	3 cut(s)	651, 665, 1210
NspI	RCATGY	2 cut(s)	317, 502
NspV	TTCGAA	1 cut(s)	1630
PagI	TCATGA	1 cut(s)	973
PdmI	GAANNNNTTC	1 cut(s)	459
PfeI	GAWTC	4 cut(s)	637, 1669, 1682, 1694
Pfl23II	CGTACG	1 cut(s)	954
PflFI	GACNNNGTC	1 cut(s)	1601
PflMI	CCANNNNNTGG	1 cut(s)	1313
PfoI	TCCNGGA	1 cut(s)	1090
PinAI	ACCGGT	1 cut(s)	1712
PkrI	GCNGC	2 cut(s)	504, 1357
PleI	GAGTC	2 cut(s)	887, 1857
PluTI	GGCGCC	1 cut(s)	881
PpsI	GAGTC	2 cut(s)	887, 1857
Ppu21I	YACGTR	3 cut(s)	1039, 1184, 1575
PpuMI	RGGWCCY	2 cut(s)	172, 802
PsiI	TTATAA	2 cut(s)	47, 344
Psp1406I	AACGTT	1 cut(s)	1653
Psp5II	RGGWCCY	2 cut(s)	172, 802
Psp6I	CCWGG	5 cut(s)	85, 428, 1216, 1538, 1857
PspFI	CCCAGC	1 cut(s)	698
PspGI	CCWGG	5 cut(s)	85, 428, 1216, 1538, 1857
PspLI	CGTACG	1 cut(s)	954
PspN4I	GGNNCC	6 cut(s)	173, 299, 669, 879, 1086, 1215
PspPI	GGNCC	8 cut(s)	172, 722, 755, 802, 851, 1316, 1611, 1892
PspPPI	RGGWCCY	2 cut(s)	172, 802
PstNI	CAGNNNCTG	1 cut(s)	640
PsuI	RGATCY	2 cut(s)	667, 1375
PsyI	GACNNNGTC	1 cut(s)	1601
RsaI	GTAC	5 cut(s)	467, 942, 956, 1037, 1788
RsaNI	GTAC	5 cut(s)	466, 941, 955, 1036, 1787
SaqAI	TTAA	5 cut(s)	228, 414, 729, 870, 1254
SatI	GCNGC	2 cut(s)	503, 1356
Sau96I	GGNCC	8 cut(s)	172, 722, 755, 802, 851, 1316, 1611, 1892
SchI	GAGTC	2 cut(s)	887, 1857
ScrFI	CCNGG	7 cut(s)	87, 430, 1092, 1218, 1540, 1791, 1859
SduI	GDGCHC	3 cut(s)	393, 787, 1847
SexAI	ACCWGGT	1 cut(s)	85
SfaNI	GCATC	5 cut(s)	129, 874, 904, 977, 1347
SfcI	CTRYAG	1 cut(s)	1664
SfoI	GGCGCC	1 cut(s)	879
SfuI	TTCGAA	1 cut(s)	1630
SinI	GGWCC	6 cut(s)	172, 722, 802, 1316, 1611, 1892
SnaBI	TACGTA	1 cut(s)	1039
SsiI	CCGC	4 cut(s)	320, 479, 503, 1263
SspDI	GGCGCC	1 cut(s)	877
SspMI	CTAG	2 cut(s)	1143, 1512
StyD4I	CCNGG	7 cut(s)	85, 428, 1090, 1216, 1538, 1789, 1857
StyI	CCWWGG	2 cut(s)	833, 1307
TaaI	ACNGT	3 cut(s)	247, 1067, 1492
TaiI	ACGT	6 cut(s)	1041, 1186, 1260, 1577, 1656, 1788
TaqI	TCGA	3 cut(s)	603, 1237, 1630
TaqII	GACCGA	1 cut(s)	531
TatI	WGTACW	1 cut(s)	940
TauI	GCSGC	1 cut(s)	505
TfiI	GAWTC	4 cut(s)	637, 1669, 1682, 1694
Tru1I	TTAA	5 cut(s)	228, 414, 729, 870, 1254
Tru9I	TTAA	5 cut(s)	228, 414, 729, 870, 1254
TscAI	CASTG	1 cut(s)	787
TseFI	GTSAC	1 cut(s)	900
TseI	GCWGC	1 cut(s)	1355
Tsp45I	GTSAC	1 cut(s)	900
TspDTI	ATGAA	3 cut(s)	1260, 1314, 1745
TspGWI	ACGGA	1 cut(s)	1871
TspRI	CASTG	1 cut(s)	787
Tth111I	GACNNNGTC	1 cut(s)	1601
Van91I	CCANNNNNTGG	1 cut(s)	1313
VpaK11BI	GGWCC	6 cut(s)	172, 722, 802, 1316, 1611, 1892
XapI	RAATTY	3 cut(s)	587, 1361, 1752
XceI	RCATGY	2 cut(s)	317, 502
XcmI	CCANNNNNNNNNTGG	1 cut(s)	831
XmiI	GTMKAC	1 cut(s)	1775
XmnI	GAANNNNTTC	1 cut(s)	459
XspI	CTAG	2 cut(s)	1143, 1512
Zsp2I	ATGCAT	3 cut(s)	651, 665, 1210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.