MD08G1248800.v1.1

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004707
Physical Location & Seq
Reverse (-)
31333488 .. 31343398
9911 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1248800.v1.1.491

Sequence Viewer

Length: 2478 bp
ATGGAAGAAGAAACCCTAAACCCCCTTACTTCTGAACAAGGCAACAATGACGACCAATCCATGCCGGACCTCCCTGACCAAGACCTTGGCTCCTCCGATTCCTATTCCGACTCCGATTCTGGCTCTGAGGATGGCGACGACCAAGCCCACCAAAACCTCCAACTCCAAACCCTAGAAGCCAAGCTCTCCACCAATCCTGCCAACTACGATACTCATGTCCAATACGTAAAGATTCTGAGGCAAATGGCTGACATTGAAAAGCTAAGGCAAGCAAGGGAAGCTATGAACCAACTGTTTCCGTTAACCCCTTCTATGTGGCAGGATTGGGCTAAGGACGATGCTTCTCTTTCCACTGGGTCTGATGCTTTTTTGGCTATTGAAAAGCTTTACGAGCGAGGAGTCTTTGAATATCTGTCCATCTCTCTTTGGCGCGACTTCTTAGATTTTGTTAAAGAGAATGATCCCTCAATAAGAGAATGTTCACCTGCTGGTATTTCGAAGGCAAGAGATCTATTTGAACGTGCTCTTACTGCAGCTGGTTTGCATGTTTCTGAAGGCAATAAACTATGGGAAGCCTACCGGTCATTTGAGCTTGCTATATTTCATACTATTGATGAGAAAGACAATCAGGCAAGAGAAAAGCAAATTCAGCGTATCCGCTCTTTATTCCACCGTCAGTTGTCAGTCCCCCATGTTAACATGAGGTCAACACTTTTGGATTACAAGGCATGGGAAGTGGAACAAGGAAACATTCTTGATGCTGGCTCCAGTGACCTGGATGGAATTTCATTCCAGGTTGCCTCAGCATATAAAAAGGCATTGGAAATGTATGATGCTCGCGTTCATCTTGAGGAACAGATTTGTCGGCAGGATATGTCGGACTCGGAAAGACTTCAAAATTTCATGAACTACTTGAGATTTGAACAGTCTTCCGGAGACCCAGCCCGGGTTCAGATGTTGTATGAACGTGCTATTACTGACTTTCCTATATCCAGTTATCTCTGGCTTGATTATACTCACTATTTGGACAAAACATTGAAGGTTGGCAATGTTATAACCAACGTTTATTCCAGGGCTGTAATGAATTGCCCCTCTGTTGGAGAGCTTTGGGTCCGATATATGCTTTCCTTGGAGCGTGGTCATGCTTCTGAGAAAGAGATTGCCTCTGTATTTGAGAAGTCTCTGCTATGCACTTTAACCCTTGATGAGTACCTAGATTTGTACCTCACACGCGTAGATAGCTTAAGAAGAAGAATCTCATGCGCCATTGAAGGAGAATATGGGTTGGAGTATTCATTAATCAGGGAAACCTTTCAGAATGCATCAGATTACTTGTCAGCATACCTGAAGAATACGGATGGCTTGCTACGTTTGCATGCTTATTGGGCCCGTTTAGAGTTAAACCTGCATAAGGACCAACTTGCAGCTCGTGGAGTCTGGGAGACTTTACTTAAGCTCTGTGGTTCAATGCTGGAAGCCTGGCAAGGTTATATAGCAATGGAAACTGAATTGGGTCATGTTAATGAAGCTCGGGCCCTATATAGGAGATGTTACAGTAAAAGATTTCTCGGAACTGGTTCAGAGGACATCTGCCACTCGTGGTTACGTTTTGAGAGGGAGTTTGGTTCGTTGGATGATTTTGACCATGCAGTACAGAAGGTCACTCCTCGTCTGGAAGAGCTAGAATTATTAAAGTCACAACAAGAATCCAAATTGACAGAAGAGAATCCTTTGAAGAAGAATGCTCGTGACAAGAGAAAGCAAGTTTCTGACGTACCTAATGAACAACATCCAGCAAAGAGGCAAAAAGATGCAGCTCAAAAGCCTAAGAAAGCACATGGGAAGGATTCTGAGGTGATGAACTCAGTTGAACAAACTAAAGTGGAAAAGACCAAGGCCACGGGTGACCAACCAGATATAGAAAATGAGCAACAGATCAAAAAGCGTGGTCCAGAGAAAAGAAAAGTATATGCCGACCAGTGTACTGTTTTTGTTTCAAATATTAACCTTAAGGCAAATGATGAACATTTGCGTCAATTCTTCAGTGATGTTGGGGGAGTGGTTGCTATAAGAATACTACATGACAAGTTCACTGGAAAGTCAAGGGGACTGGCATATGTGGATTTCTCAGATGATGGCCACCTCGTTGCAGCTGTTGCAAAAAATAAGCAGGTGCTGCTTGGGAAGAAGCTGAGCATTGCACGATCAGATCCAAAGCATGGGAAGAAGGAACATGCCAGGGGAGCCGAGTCTAATAATTCTGCTGAGGCCTCCAATGCGCCTAGGGCACGTCAAGCACAGCAGTCTACCTATAACCGAGGAAGCAATAATGTACAGCTGAGGGGTAAGAACACATTTGCAACTCCCAGGAATATCTTTGCACTTGGTCATAGTAGGAATAAACCAAAAACTGAGGAACAGGATGATGAAAAGCCAAAATCCAATGATGAGTTCAGAAACATGTTCCGCAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

826

Amino Acids

94.37

Weight (kDa)

5.9

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 55 - 560 1.7e-32 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 58 - 241 1.5e-16 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 283 - 393 2.6e-09 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 293 - 361 1.7e-11 PRP39 C-terminal HAT repeat
HAT_PRP39_N PF23240 302 - 397 7.3e-07 PRP39 N-terminal HAT repeat
RRM_1 PF00076 663 - 731 2.7e-13 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1055
AarI CACCTGC 2 cut(s) 493, 2161
Acc36I ACCTGC 3 cut(s) 493, 1413, 2161
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 2306
AccII CGCG 3 cut(s) 432, 840, 1233
AccIII TCCGGA 1 cut(s) 932
AciI CCGC 2 cut(s) 658, 2467
AclI AACGTT 1 cut(s) 1062
AclWI GGATC 2 cut(s) 455, 2204
AcoI YGGCCR 1 cut(s) 2137
AcsI RAATTY 3 cut(s) 645, 783, 898
AcuI CTGAAG 3 cut(s) 573, 1367, 2026
AfaI GTAC 6 cut(s) 1211, 1223, 1653, 1776, 1984, 2334
AfiI CCNNNNNNNGG 5 cut(s) 946, 1097, 1411, 1542, 2219
AflII CTTAAG 3 cut(s) 1243, 1451, 2009
AflIII ACRYGT 2 cut(s) 1231, 2460
AgeI ACCGGT 1 cut(s) 579
AjiI CACGTC 1 cut(s) 2291
AjnI CCWGG 6 cut(s) 774, 792, 1070, 1478, 2237, 2366
AjuI GAANNNNNNNTTGG 2 cut(s) 2435, 2467
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 3 cut(s) 930, 1185, 1436
AlwI GGATC 2 cut(s) 455, 2204
AlwNI CAGNNNCTG 1 cut(s) 2176
Ama87I CYCGRG 2 cut(s) 945, 1530
Aor13HI TCCGGA 1 cut(s) 932
AoxI GGCC 5 cut(s) 1386, 1533, 1896, 2137, 2268
ApaI GGGCCC 2 cut(s) 1390, 1537
ApeKI GCWGC 5 cut(s) 533, 1424, 1814, 2150, 2176
ApoI RAATTY 3 cut(s) 645, 783, 898
AseI ATTAAT 1 cut(s) 1298
AsiGI ACCGGT 1 cut(s) 579
Asp700I GAANNNNTTC 3 cut(s) 891, 1311, 1576
AspA2I CCTAGG 1 cut(s) 2282
AspLEI GCGC 3 cut(s) 432, 1265, 2281
AspS9I GGNCC 8 cut(s) 67, 1111, 1386, 1387, 1414, 1533, 1534, 1949
AsuC2I CCSGG 2 cut(s) 946, 947
AsuHPI GGTGA 3 cut(s) 474, 1867, 1916
AsuII TTCGAA 1 cut(s) 497
AvaI CYCGRG 2 cut(s) 945, 1530
AvaII GGWCC 4 cut(s) 67, 1111, 1414, 1949
AvrII CCTAGG 1 cut(s) 2282
BaeGI GKGCMC 3 cut(s) 1390, 1537, 2290
BaeI ACNNNNGTAYC 2 cut(s) 201, 234
BalI TGGCCA 1 cut(s) 2139
BanII GRGCYC 2 cut(s) 1390, 1537
BauI CACGAG 3 cut(s) 1428, 1597, 1746
BbsI GAAGAC 1 cut(s) 921
Bbv12I GWGCWC 1 cut(s) 526
BbvCI CCTCAGC 3 cut(s) 802, 2265, 2339
BbvI GCAGC 5 cut(s) 545, 1436, 1826, 2162, 2163
BccI CCATC 5 cut(s) 125, 425, 773, 1352, 2129
BciT130I CCWGG 6 cut(s) 776, 794, 1072, 1480, 2239, 2368
BciVI GTATCC 1 cut(s) 665
BcnI CCSGG 2 cut(s) 946, 947
BcoDI GTCTC 3 cut(s) 930, 1185, 1436
BfaI CTAG 4 cut(s) 173, 1214, 1682, 2283
BfmI CTRYAG 1 cut(s) 531
BfrI CTTAAG 3 cut(s) 1243, 1451, 2009
BfuAI ACCTGC 3 cut(s) 493, 1413, 2161
BfuI GTATCC 1 cut(s) 665
BglII AGATCT 1 cut(s) 508
BisI GCNGC 5 cut(s) 534, 1425, 1815, 2151, 2177
BlnI CCTAGG 1 cut(s) 2282
BlpI GCTNAGC 1 cut(s) 2192
BlsI GCNGC 5 cut(s) 535, 1426, 1816, 2152, 2178
Bme1390I CCNGG 8 cut(s) 776, 794, 946, 947, 1072, 1480, 2239, 2368
Bme18I GGWCC 4 cut(s) 67, 1111, 1414, 1949
BmeT110I CYCGRG 2 cut(s) 945, 1530
BmgBI CACGTC 1 cut(s) 2291
BmgT120I GGNCC 8 cut(s) 67, 1111, 1386, 1387, 1414, 1533, 1534, 1949
BmiI GGNNCC 6 cut(s) 91, 766, 1112, 1388, 1535, 2245
BmrFI CCNGG 8 cut(s) 776, 794, 946, 947, 1072, 1480, 2239, 2368
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 6 cut(s) 328, 352, 748, 823, 1331, 1801
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 921
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 1 cut(s) 751
Bpu10I CCTNAGC 5 cut(s) 263, 330, 802, 2265, 2339
Bpu1102I GCTNAGC 1 cut(s) 2192
Bpu14I TTCGAA 1 cut(s) 497
BpuEI CTTGAG 2 cut(s) 869, 934
BpuMI CCSGG 2 cut(s) 946, 947
BsaAI YACGTR 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 930
BsaWI WCCGGW 2 cut(s) 579, 932
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 5 cut(s) 946, 1097, 1411, 1542, 2219
Bse118I RCCGGY 1 cut(s) 579
Bse1I ACTGG 7 cut(s) 358, 768, 993, 1579, 1978, 2098, 2115
Bse3DI GCAATG 3 cut(s) 1054, 1503, 2196
BseAI TCCGGA 1 cut(s) 932
BseBI CCWGG 6 cut(s) 776, 794, 1072, 1480, 2239, 2368
BseGI GGATG 6 cut(s) 136, 784, 1363, 1639, 1789, 2428
BseLI CCNNNNNNNGG 5 cut(s) 946, 1097, 1411, 1542, 2219
BseMI GCAATG 3 cut(s) 1054, 1503, 2196
BseNI ACTGG 7 cut(s) 358, 768, 993, 1579, 1978, 2098, 2115
BseRI GAGGAG 3 cut(s) 82, 411, 1656
BseSI GKGCMC 3 cut(s) 1390, 1537, 2290
BseXI GCAGC 5 cut(s) 545, 1436, 1826, 2162, 2163
BseYI CCCAGC 1 cut(s) 940
Bsh1236I CGCG 3 cut(s) 432, 840, 1233
BshFI GGCC 5 cut(s) 1388, 1535, 1898, 2139, 2270
BshTI ACCGGT 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 526
BsiHKCI CYCGRG 2 cut(s) 945, 1530
BsiSI CCGG 4 cut(s) 65, 580, 933, 946
BslFI GGGAC 2 cut(s) 671, 2121
BslI CCNNNNNNNGG 5 cut(s) 946, 1097, 1411, 1542, 2219
BsmAI GTCTC 3 cut(s) 930, 1185, 1436
BsmFI GGGAC 2 cut(s) 671, 2121
BsmI GAATGC 2 cut(s) 1324, 1747
BsnI GGCC 5 cut(s) 1388, 1535, 1898, 2139, 2270
Bso31I GGTCTC 1 cut(s) 930
BsoBI CYCGRG 2 cut(s) 945, 1530
Bsp119I TTCGAA 1 cut(s) 497
Bsp120I GGGCCC 2 cut(s) 1386, 1533
Bsp1286I GDGCHC 4 cut(s) 526, 1390, 1537, 2290
Bsp13I TCCGGA 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 2332
Bsp143I GATC 5 cut(s) 460, 508, 1935, 2204, 2209
Bsp1720I GCTNAGC 1 cut(s) 2192
BspACI CCGC 2 cut(s) 658, 2467
BspANI GGCC 5 cut(s) 1388, 1535, 1898, 2139, 2270
BspEI TCCGGA 1 cut(s) 932
BspFNI CGCG 3 cut(s) 432, 840, 1233
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 6 cut(s) 91, 766, 1112, 1388, 1535, 2245
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 3 cut(s) 493, 1413, 2161
BspPI GGATC 2 cut(s) 455, 2204
BspQI GCTCTTC 1 cut(s) 1671
BspT104I TTCGAA 1 cut(s) 497
BspTI CTTAAG 3 cut(s) 1243, 1451, 2009
BspTNI GGTCTC 1 cut(s) 930
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 3 cut(s) 1054, 1503, 2196
BsrFI RCCGGY 1 cut(s) 579
BsrGI TGTACA 1 cut(s) 2332
BsrI ACTGG 7 cut(s) 358, 768, 993, 1579, 1978, 2098, 2115
BssAI RCCGGY 1 cut(s) 579
BssMI GATC 5 cut(s) 460, 508, 1935, 2204, 2209
BssSI CACGAG 3 cut(s) 1428, 1597, 1746
BssT1I CCWWGG 4 cut(s) 85, 1128, 1893, 2282
Bst2BI CACGAG 3 cut(s) 1428, 1597, 1746
Bst2UI CCWGG 6 cut(s) 776, 794, 1072, 1480, 2239, 2368
Bst4CI ACNGT 5 cut(s) 294, 674, 927, 1556, 1987
Bst6I CTCTTC 2 cut(s) 1671, 1716
BstAFI CTTAAG 3 cut(s) 1243, 1451, 2009
BstAPI GCANNNNNTGC 2 cut(s) 2156, 2176
BstAUI TGTACA 1 cut(s) 2332
BstBAI YACGTR 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 497
BstC8I GCNNGC 6 cut(s) 270, 594, 763, 838, 1364, 1377
BstDSI CCRYGG 1 cut(s) 1899
BstEII GGTNACC 1 cut(s) 1904
BstENI CCTNNNNNAGG 1 cut(s) 1409
BstF5I GGATG 6 cut(s) 136, 784, 1363, 1639, 1789, 2428
BstFNI CGCG 3 cut(s) 432, 840, 1233
BstHHI GCGC 3 cut(s) 432, 1265, 2281
BstKTI GATC 5 cut(s) 463, 511, 1938, 2207, 2212
BstMAI GTCTC 3 cut(s) 930, 1185, 1436
BstMBI GATC 5 cut(s) 460, 508, 1935, 2204, 2209
BstNI CCWGG 6 cut(s) 776, 794, 1072, 1480, 2239, 2368
BstNSI RCATGY 4 cut(s) 548, 1379, 2237, 2464
BstPI GGTNACC 1 cut(s) 1904
BstSCI CCNGG 8 cut(s) 774, 792, 944, 945, 1070, 1478, 2237, 2366
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 3 cut(s) 1390, 1537, 2290
BstSNI TACGTA 1 cut(s) 226
BstUI CGCG 3 cut(s) 432, 840, 1233
BstV1I GCAGC 5 cut(s) 545, 1436, 1826, 2162, 2163
BstV2I GAAGAC 1 cut(s) 921
BstX2I RGATCY 2 cut(s) 508, 2209
BstXI CCANNNNNNTGG 2 cut(s) 86, 775
BstYI RGATCY 2 cut(s) 508, 2209
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 5 cut(s) 1388, 1535, 1898, 2139, 2270
BtgI CCRYGG 1 cut(s) 1899
BtrI CACGTC 1 cut(s) 2291
BtsCI GGATG 6 cut(s) 136, 784, 1363, 1639, 1789, 2428
BtsIMutI CAGTG 5 cut(s) 351, 775, 1985, 2050, 2091
BveI ACCTGC 3 cut(s) 493, 1413, 2161
Cac8I GCNNGC 6 cut(s) 270, 594, 763, 838, 1364, 1377
CaiI CAGNNNCTG 1 cut(s) 2176
CciI TCATGA 1 cut(s) 903
CfoI GCGC 3 cut(s) 432, 1265, 2281
Cfr10I RCCGGY 1 cut(s) 579
Cfr13I GGNCC 8 cut(s) 67, 1111, 1386, 1387, 1414, 1533, 1534, 1949
Cfr9I CCCGGG 1 cut(s) 945
CseI GACGC 1 cut(s) 2021
Csp6I GTAC 6 cut(s) 1210, 1222, 1652, 1775, 1983, 2333
CspAI ACCGGT 1 cut(s) 579
CspCI CAANNNNNGTGG 2 cut(s) 1927, 1962
CviQI GTAC 6 cut(s) 1210, 1222, 1652, 1775, 1983, 2333
DpnI GATC 5 cut(s) 462, 510, 1937, 2206, 2211
DpnII GATC 5 cut(s) 460, 508, 1935, 2204, 2209
EaeI YGGCCR 1 cut(s) 2137
Eam1104I CTCTTC 2 cut(s) 1671, 1716
EarI CTCTTC 2 cut(s) 1671, 1716
Eco105I TACGTA 1 cut(s) 226
Eco130I CCWWGG 4 cut(s) 85, 1128, 1893, 2282
Eco147I AGGCCT 1 cut(s) 2270
Eco24I GRGCYC 2 cut(s) 1390, 1537
Eco31I GGTCTC 1 cut(s) 930
Eco47I GGWCC 4 cut(s) 67, 1111, 1414, 1949
Eco57I CTGAAG 3 cut(s) 573, 1367, 2026
Eco88I CYCGRG 2 cut(s) 945, 1530
Eco91I GGTNACC 1 cut(s) 1904
EcoNI CCTNNNNNAGG 1 cut(s) 1409
EcoO109I RGGNCCY 1 cut(s) 1534
EcoO65I GGTNACC 1 cut(s) 1904
EcoRII CCWGG 6 cut(s) 774, 792, 1070, 1478, 2237, 2366
EcoT14I CCWWGG 4 cut(s) 85, 1128, 1893, 2282
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 2 cut(s) 1390, 1537
ErhI CCWWGG 4 cut(s) 85, 1128, 1893, 2282
FaqI GGGAC 2 cut(s) 671, 2121
FauNDI CATATG 1 cut(s) 2116
FblI GTMKAC 1 cut(s) 2306
Fnu4HI GCNGC 5 cut(s) 534, 1425, 1815, 2151, 2177
FokI GGATG 6 cut(s) 143, 791, 1370, 1646, 1776, 2435
FriOI GRGCYC 2 cut(s) 1390, 1537
Fsp4HI GCNGC 5 cut(s) 534, 1425, 1815, 2151, 2177
FspBI CTAG 4 cut(s) 173, 1214, 1682, 2283
GlaI GCGC 3 cut(s) 431, 1264, 2280
GluI GCNGC 5 cut(s) 534, 1425, 1815, 2151, 2177
GsaI CCCAGC 1 cut(s) 944
GsuI CTGGAG 1 cut(s) 751
HaeIII GGCC 5 cut(s) 1388, 1535, 1898, 2139, 2270
HapII CCGG 4 cut(s) 65, 580, 933, 946
HgaI GACGC 1 cut(s) 2021
HhaI GCGC 3 cut(s) 432, 1265, 2281
Hin6I GCGC 3 cut(s) 430, 1263, 2279
HinP1I GCGC 3 cut(s) 430, 1263, 2279
HincII GTYRAC 3 cut(s) 303, 697, 708
HindII GTYRAC 3 cut(s) 303, 697, 708
HindIII AAGCTT 1 cut(s) 383
HpaI GTTAAC 2 cut(s) 303, 697
HpaII CCGG 4 cut(s) 65, 580, 933, 946
HphI GGTGA 3 cut(s) 474, 1867, 1916
Hpy166II GTNNAC 7 cut(s) 303, 482, 697, 708, 1983, 2091, 2307
Hpy188III TCNNGA 7 cut(s) 755, 848, 904, 933, 1673, 1748, 1952
Hpy8I GTNNAC 7 cut(s) 303, 482, 697, 708, 1983, 2091, 2307
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 8 cut(s) 318, 493, 548, 1033, 1265, 1651, 1837, 2221
HpyCH4III ACNGT 5 cut(s) 294, 674, 927, 1556, 1987
HpyCH4IV ACGT 8 cut(s) 225, 520, 967, 1062, 1369, 1606, 1773, 2290
HpySE526I ACGT 8 cut(s) 225, 520, 967, 1062, 1369, 1606, 1773, 2290
HspAI GCGC 3 cut(s) 430, 1263, 2279
Kpn2I TCCGGA 1 cut(s) 932
KspAI GTTAAC 2 cut(s) 303, 697
Kzo9I GATC 5 cut(s) 460, 508, 1935, 2204, 2209
LguI GCTCTTC 1 cut(s) 1671
LmnI GCTCC 4 cut(s) 95, 770, 1132, 2243
Lsp1109I GCAGC 5 cut(s) 545, 1436, 1826, 2162, 2163
LweI GCATC 6 cut(s) 328, 352, 748, 823, 1331, 1801
MaeI CTAG 4 cut(s) 173, 1214, 1682, 2283
MaeII ACGT 8 cut(s) 225, 520, 967, 1062, 1369, 1606, 1773, 2290
MaeIII GTNAC 7 cut(s) 770, 1550, 1602, 1660, 1695, 1748, 1904
MalI GATC 5 cut(s) 462, 510, 1937, 2206, 2211
MbiI CCGCTC 1 cut(s) 660
MboI GATC 5 cut(s) 460, 508, 1935, 2204, 2209
MflI RGATCY 2 cut(s) 508, 2209
MhlI GDGCHC 4 cut(s) 526, 1390, 1537, 2290
MlsI TGGCCA 1 cut(s) 2139
MluCI AATT 9 cut(s) 645, 783, 898, 1084, 1508, 1685, 1712, 2036, 2257
MluI ACGCGT 1 cut(s) 1231
MluNI TGGCCA 1 cut(s) 2139
MlyI GAGTC 5 cut(s) 104, 408, 875, 1443, 2258
MmeI TCCRAC 6 cut(s) 132, 184, 858, 1078, 1266, 1611
Mox20I TGGCCA 1 cut(s) 2139
Mph1103I ATGCAT 1 cut(s) 1324
MroI TCCGGA 1 cut(s) 932
MroXI GAANNNNTTC 3 cut(s) 891, 1311, 1576
MscI TGGCCA 1 cut(s) 2139
MslI CAYNNNNRTG 1 cut(s) 1521
Msp20I TGGCCA 1 cut(s) 2139
MspA1I CMGCKG 3 cut(s) 536, 2153, 2338
MspCI CTTAAG 3 cut(s) 1243, 1451, 2009
MspI CCGG 4 cut(s) 65, 580, 933, 946
MspR9I CCNGG 8 cut(s) 776, 794, 946, 947, 1072, 1480, 2239, 2368
Mva1269I GAATGC 2 cut(s) 1324, 1747
MvaI CCWGG 6 cut(s) 776, 794, 1072, 1480, 2239, 2368
MvnI CGCG 3 cut(s) 432, 840, 1233
NciI CCSGG 2 cut(s) 946, 947
NdeI CATATG 1 cut(s) 2116
NdeII GATC 5 cut(s) 460, 508, 1935, 2204, 2209
NlaIV GGNNCC 6 cut(s) 91, 766, 1112, 1388, 1535, 2245
NmeAIII GCCGAG 1 cut(s) 2272
NmuCI GTSAC 5 cut(s) 770, 1660, 1695, 1748, 1904
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 4 cut(s) 548, 1379, 2237, 2464
NspV TTCGAA 1 cut(s) 497
PaeI GCATGC 1 cut(s) 1379
PagI TCATGA 1 cut(s) 903
PaqCI CACCTGC 2 cut(s) 493, 2161
PceI AGGCCT 1 cut(s) 2270
PciI ACATGT 1 cut(s) 2460
PciSI GCTCTTC 1 cut(s) 1671
PctI GAATGC 2 cut(s) 1324, 1747
PdmI GAANNNNTTC 3 cut(s) 891, 1311, 1576
PfeI GAWTC 7 cut(s) 98, 116, 232, 1254, 1706, 1726, 1847
PflMI CCANNNNNTGG 1 cut(s) 2219
PinAI ACCGGT 1 cut(s) 579
PkrI GCNGC 5 cut(s) 535, 1426, 1816, 2152, 2178
PleI GAGTC 5 cut(s) 104, 407, 875, 1442, 2257
PpsI GAGTC 5 cut(s) 104, 407, 875, 1442, 2257
Ppu21I YACGTR 1 cut(s) 226
PscI ACATGT 1 cut(s) 2460
PshBI ATTAAT 1 cut(s) 1298
PsiI TTATAA 1 cut(s) 1055
Psp1406I AACGTT 1 cut(s) 1062
Psp6I CCWGG 6 cut(s) 774, 792, 1070, 1478, 2237, 2366
PspEI GGTNACC 1 cut(s) 1904
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 6 cut(s) 774, 792, 1070, 1478, 2237, 2366
PspN4I GGNNCC 6 cut(s) 91, 766, 1112, 1388, 1535, 2245
PspOMI GGGCCC 2 cut(s) 1386, 1533
PspPI GGNCC 8 cut(s) 67, 1111, 1386, 1387, 1414, 1533, 1534, 1949
PstI CTGCAG 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 2176
PsuI RGATCY 2 cut(s) 508, 2209
PvuII CAGCTG 3 cut(s) 536, 2153, 2338
RsaI GTAC 6 cut(s) 1211, 1223, 1653, 1776, 1984, 2334
RsaNI GTAC 6 cut(s) 1210, 1222, 1652, 1775, 1983, 2333
RseI CAYNNNNRTG 1 cut(s) 1521
SapI GCTCTTC 1 cut(s) 1671
SatI GCNGC 5 cut(s) 534, 1425, 1815, 2151, 2177
Sau3AI GATC 5 cut(s) 460, 508, 1935, 2204, 2209
Sau96I GGNCC 8 cut(s) 67, 1111, 1386, 1387, 1414, 1533, 1534, 1949
SchI GAGTC 5 cut(s) 104, 408, 875, 1443, 2258
ScrFI CCNGG 8 cut(s) 776, 794, 946, 947, 1072, 1480, 2239, 2368
SduI GDGCHC 4 cut(s) 526, 1390, 1537, 2290
SfaNI GCATC 6 cut(s) 328, 352, 748, 823, 1331, 1801
SfcI CTRYAG 1 cut(s) 531
SfuI TTCGAA 1 cut(s) 497
SinI GGWCC 4 cut(s) 67, 1111, 1414, 1949
SmaI CCCGGG 1 cut(s) 947
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 5 cut(s) 848, 913, 1243, 1451, 2009
SmoI CTYRAG 5 cut(s) 848, 913, 1243, 1451, 2009
SnaBI TACGTA 1 cut(s) 226
SphI GCATGC 1 cut(s) 1379
Sse9I AATT 9 cut(s) 645, 783, 898, 1084, 1508, 1685, 1712, 2036, 2257
SseBI AGGCCT 1 cut(s) 2270
SsiI CCGC 2 cut(s) 658, 2467
SspI AATATT 1 cut(s) 2002
SspMI CTAG 4 cut(s) 173, 1214, 1682, 2283
StuI AGGCCT 1 cut(s) 2270
StyD4I CCNGG 8 cut(s) 774, 792, 944, 945, 1070, 1478, 2237, 2366
StyI CCWWGG 4 cut(s) 85, 1128, 1893, 2282
TaaI ACNGT 5 cut(s) 294, 674, 927, 1556, 1987
TaiI ACGT 8 cut(s) 228, 523, 970, 1065, 1372, 1609, 1776, 2293
TaqI TCGA 1 cut(s) 497
TasI AATT 9 cut(s) 645, 783, 898, 1084, 1508, 1685, 1712, 2036, 2257
TatI WGTACW 3 cut(s) 1651, 1982, 2332
TfiI GAWTC 7 cut(s) 98, 116, 232, 1254, 1706, 1726, 1847
TscAI CASTG 5 cut(s) 358, 775, 1985, 2050, 2098
TseFI GTSAC 5 cut(s) 770, 1660, 1695, 1748, 1904
TseI GCWGC 5 cut(s) 533, 1424, 1814, 2150, 2176
Tsp45I GTSAC 5 cut(s) 770, 1660, 1695, 1748, 1904
TspGWI ACGGA 2 cut(s) 288, 1370
TspMI CCCGGG 1 cut(s) 945
TspRI CASTG 5 cut(s) 358, 775, 1985, 2050, 2098
Van91I CCANNNNNTGG 1 cut(s) 2219
Vha464I CTTAAG 3 cut(s) 1243, 1451, 2009
VpaK11BI GGWCC 4 cut(s) 67, 1111, 1414, 1949
VspI ATTAAT 1 cut(s) 1298
XagI CCTNNNNNAGG 1 cut(s) 1409
XapI RAATTY 3 cut(s) 645, 783, 898
XceI RCATGY 4 cut(s) 548, 1379, 2237, 2464
XmaI CCCGGG 1 cut(s) 945
XmaJI CCTAGG 1 cut(s) 2282
XmiI GTMKAC 1 cut(s) 2306
XmnI GAANNNNTTC 3 cut(s) 891, 1311, 1576
XspI CTAG 4 cut(s) 173, 1214, 1682, 2283
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.