Rh7AG324000

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004707
Physical Location & Seq
Forward (+)
36715016 .. 36723065
8050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG324000.1

Sequence Viewer

Length: 2466 bp
ATGGAGGAAGAAACCCTAAATCCTGAACCAGAGACCGTGACTTCGCAAGAGAACAACGGAGACCAATCCATGCTGGATGCCGCTGACCAAAATCCTAACTCCTCCAGCTCCGATTCTGATTCCGACTCCGGCTCCGATGACGAAGACCAAGCCCAGCAAAACCTGGAGATTCAAACACTCGAAGCCGAGCTCGCAACCAACCCCTCCAACTACGATGCCCATGTCCAATACATTCAGATTCTGAGGAAAATGGCTGACATTGAAAAGCTGAGGCGAGCGAGGGAAGCTATGAGTATGCTGTTGCCGTTAACCCCCTCCATGTGGCAGGATTGGGCTAAGGACGAACTTGCTATGGGCTCCGGACCCGAGACGTTTTCTACAGTTGAAAAGCTTTATGAGCAAGGAGTCTCTGATTATCTGTCCATCTCTCTTTGGCGAGACTACTTAAATTTTGTTCGAGAAAATGATCCGTCAGTAAAAGAATCTTCACCTGCTGGTATTTCCAAGGAAAGAGATTTATTTGAGCGTGCTCTTACTGCAGCTGGTTTGCATGTATCTGAAGGCAATAAGATATGGGAAGCTTACAGGGAGTTTGAGCAAGCTATATGTGATACTATCGATGGGACTGACACTCAGGCAAGAGAGAAGCAAATTCAGCGCATTCGTGTTATATTCCAACGTCAGCTATCAGTTCCCCATGTTAATATGAGATCAACACTTTTGGCTTACAAGGCATGGGAGATGGAACAAGGAACCATTGTTGACACTGGCTGCAGTGATCAGGATGGTATTTCATCTCATGTTGCCGCAGCATACCAAAAGGCATTGGAAATTTATAACGCCCGTGTTCATTTTGAAGAACAAATCAGTCAGCATGATATGCCTGAGATAGAAAGACTTGAAAAGTTCATGAACTACTTGAAATTTGAGCAATCTTCTGGAGATCCTGCCCGTGTTCAAGTGTTGTATGAACGGGCTATTGCTGAGTTTCCTATAGCCAGTCAGCTCTGGCTTGATTATACTTCCTATTTGGACAAAACTTTGAAGGTTGGCAGAATTATAAGCAACGTTTATTCTAGGGCTGTAAAGAATTGCCCCTGGGTTGGAGAACTTTGGGTCAGATATTTGCTTTCCTTGGAGCGTGGCCATGCTTCTGAGAAAGAGATTGCAGCTGTGTTTTACAAGTCCCAGCAATGCACCTTTTCAACCCTTGATGAGTACGTGGAGTTGTTTCTCACCCGCATAGATGGCTTAAGACGAAGACTATCATGCCCCGTTGAAGGAGAGCACGCCTTGGATTATTCATTGTTGAGGGATACATTTCAGTCTGCATCGGATTACCTATCACCACAGATGAAGAACACAGATGTCCTGTTACGTTTGTATGCTTATTGGGCCCGCCTAGAGCTACATCTGGGAAAAGATTTAGTTGCTGCTCGGGGGGTTTGGGAAAGTTTACTGAAGATCAGTGGTATGATGATGGAAGCCTGGCTAGGTTATATAGCAATGGAAATTGAATCGGGCCATGTGAATGAAGCTCGGTCCATATATAGGAGATGCTACAGTAAAAAATTTTCCGGCACTGGTTCAGAGGACATTTGCTACTTGTGGTTGCGCTTTGAGAGGGAGTTTGGGTCGTTGGATGATTTCGACTATGCAGAACGTAAGGTTGCTCCCCGTCTGCAAGAGCTTCAACTATTAAGGTCCAAGCAAGAATCCAAATTGACTGAAGAGAGGGAGAATTCTCTCAAAAAGAATGTGCGTGAGAAGAGAAAGCAAGCTTCAGATGTAAGTGATGAACAACCTCCAGCAAAGAGGCAAAAGGATGCAACTCAAAAGCAAAAGAAAGTGCATGGGAAGGATTATGAGGTGCAGAACAGAGCTGAACAAAATGAAGGGAAAGAGATGAAAGCCAAGGGGGCCAAAGATGTACAAAATGAGATGAAAGAGCCTGTTCTAGAGAAAACAAAAGTATACGCTGACCAATGCACTGCTTTTGTATCAAACCTTAGCTTAAAGGCAAATAATGAACATCTTCATCAGTTCTTCAGTGATGTTGGAGGAGTTGTTGCTATTCGAATACTGAAGGACAAGTTCACTGGAAATTCAAGGGGACTAGCATATGTGGACTTCTCAGATGATGCACGCCTTGCTGCAGCCATAGCAAAAAATAAACAGACACTCCTAGACAGGAAGCTGAGCATTGCACGATCAAATCCAAAGCGTGGCAAGAAAGAACATGGGCACACTGATCAAACTGGCGGAGCCAGTGGATCTGGTGAGACCTCCAATGAACCTAGAGCACGCCAAGGAAGCGACAGCGCGATTAAGAGGAAGAACACATTTGCGGTTCCGAGGAATGTTGCTGCACTTGGTTGGATCACGAATAAGCCAAAAACTCAGGATCCAGATGATGAAAAGCCAAAATCTAATGATGAGTTCAGAAACATGTTCCTGAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

821

Amino Acids

93.57

Weight (kDa)

5.55

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 57 - 393 5.7e-29 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 60 - 242 1.1e-16 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 61 - 202 6.9e-06 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 281 - 363 7.4e-12 PRP39 C-terminal HAT repeat
HAT_PRP39_N PF23240 304 - 411 9e-07 PRP39 N-terminal HAT repeat
RRM_1 PF00076 666 - 733 7.3e-14 RNA recognition motif
Lsm_interact PF05391 803 - 820 1.7e-07 Lsm interaction motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 837, 1061
AarI CACCTGC 1 cut(s) 499
Acc36I ACCTGC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 2225
AccI GTMKAC 1 cut(s) 1974
AccII CGCG 1 cut(s) 2324
AccIII TCCGGA 1 cut(s) 359
AciI CCGC 6 cut(s) 81, 807, 1240, 1399, 2262, 2348
AclI AACGTT 1 cut(s) 1068
AclWI GGATC 6 cut(s) 461, 938, 2281, 2387, 2399, 2412
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 7 cut(s) 448, 651, 831, 923, 1571, 1741, 2104
AcuI CTGAAG 6 cut(s) 579, 1481, 1749, 1767, 2032, 2105
AfaI GTAC 2 cut(s) 1220, 1932
AfiI CCNNNNNNNGG 5 cut(s) 321, 1103, 1280, 1551, 2225
AflII CTTAAG 1 cut(s) 1252
AflIII ACRYGT 1 cut(s) 2448
AjnI CCWGG 3 cut(s) 162, 1097, 1487
Alw21I GWGCWC 4 cut(s) 192, 532, 1290, 2305
Alw26I GTCTC 6 cut(s) 26, 54, 362, 412, 432, 2276
AlwI GGATC 6 cut(s) 461, 938, 2281, 2387, 2399, 2412
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 2 cut(s) 365, 1437
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 4 cut(s) 1144, 1395, 1522, 1920
ApaI GGGCCC 1 cut(s) 1399
ApeKI GCWGC 8 cut(s) 539, 771, 809, 1169, 1433, 2153, 2156, 2366
ApoI RAATTY 7 cut(s) 448, 651, 831, 923, 1571, 1741, 2104
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 2034
AspLEI GCGC 3 cut(s) 660, 1617, 2324
AspS9I GGNCC 7 cut(s) 362, 1395, 1396, 1522, 1542, 1704, 1920
AsuHPI GGTGA 4 cut(s) 480, 1228, 1338, 2291
AsuII TTCGAA 1 cut(s) 2077
AvaI CYCGRG 2 cut(s) 365, 1437
AvaII GGWCC 3 cut(s) 362, 1542, 1704
BaeGI GKGCMC 2 cut(s) 1399, 2247
BalI TGGCCA 1 cut(s) 1146
BamHI GGATCC 1 cut(s) 2404
BanII GRGCYC 3 cut(s) 192, 359, 1399
BbsI GAAGAC 2 cut(s) 150, 1267
Bbv12I GWGCWC 4 cut(s) 192, 532, 1290, 2305
BbvCI CCTCAGC 1 cut(s) 269
BbvI GCAGC 8 cut(s) 551, 758, 821, 1181, 1420, 2140, 2168, 2353
BccI CCATC 6 cut(s) 431, 614, 736, 779, 1241, 1474
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 3 cut(s) 164, 1099, 1489
BciVI GTATCC 1 cut(s) 1309
BclI TGATCA 2 cut(s) 778, 2251
BcoDI GTCTC 6 cut(s) 26, 54, 362, 412, 432, 2276
BfaI CTAG 7 cut(s) 1077, 1403, 1493, 1958, 2117, 2186, 2298
BfmI CTRYAG 6 cut(s) 378, 537, 772, 993, 1561, 2154
BfrI CTTAAG 1 cut(s) 1252
BfuAI ACCTGC 1 cut(s) 499
BfuI GTATCC 1 cut(s) 1309
BglI GCCNNNNNGGC 1 cut(s) 1919
BlpI GCTNAGC 1 cut(s) 2198
Bme1390I CCNGG 3 cut(s) 164, 1099, 1489
Bme18I GGWCC 3 cut(s) 362, 1542, 1704
BmeT110I CYCGRG 2 cut(s) 365, 1437
BmgT120I GGNCC 7 cut(s) 362, 1395, 1396, 1522, 1542, 1704, 1920
BmiI GGNNCC 9 cut(s) 133, 358, 364, 754, 1397, 1921, 2266, 2352, 2406
BmrFI CCNGG 3 cut(s) 164, 1099, 1489
BmsI GCATC 6 cut(s) 67, 205, 1340, 1547, 1816, 2131
BpiI GAAGAC 2 cut(s) 150, 1267
BpmI CTGGAG 4 cut(s) 88, 185, 960, 1791
Bpu10I CCTNAGC 3 cut(s) 269, 336, 2009
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 1 cut(s) 2077
Bsa29I ATCGAT 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 1222
BsaI GGTCTC 3 cut(s) 26, 54, 2276
BsaJI CCNNGG 8 cut(s) 504, 1097, 1098, 1134, 1293, 1914, 2308, 2354
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 8 cut(s) 116, 146, 1619, 1649, 2052, 2082, 2166, 2196
Bsc4I CCNNNNNNNGG 5 cut(s) 321, 1103, 1280, 1551, 2225
Bse1I ACTGG 6 cut(s) 772, 999, 1588, 2104, 2263, 2268
Bse3DI GCAATG 3 cut(s) 1199, 1512, 2202
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 3 cut(s) 164, 1099, 1489
BseCI ATCGAT 1 cut(s) 618
BseDI CCNNGG 8 cut(s) 504, 1097, 1098, 1134, 1293, 1914, 2308, 2354
BseGI GGATG 4 cut(s) 82, 790, 1648, 1831
BseLI CCNNNNNNNGG 5 cut(s) 321, 1103, 1280, 1551, 2225
BseMI GCAATG 3 cut(s) 1199, 1512, 2202
BseMII CTCAG 9 cut(s) 233, 260, 647, 876, 975, 1146, 2148, 2189, 2414
BseNI ACTGG 6 cut(s) 772, 999, 1588, 2104, 2263, 2268
BseRI GAGGAG 2 cut(s) 91, 2076
BseSI GKGCMC 2 cut(s) 1399, 2247
BseXI GCAGC 8 cut(s) 551, 758, 821, 1181, 1420, 2140, 2168, 2353
BseYI CCCAGC 2 cut(s) 153, 1188
BsgI GTGCAG 2 cut(s) 1892, 2352
Bsh1236I CGCG 1 cut(s) 2324
BshFI GGCC 4 cut(s) 1146, 1397, 1524, 1922
BshVI ATCGAT 1 cut(s) 618
BsiHKAI GWGCWC 4 cut(s) 192, 532, 1290, 2305
BsiHKCI CYCGRG 2 cut(s) 365, 1437
BsiSI CCGG 3 cut(s) 129, 360, 1578
BslFI GGGAC 3 cut(s) 637, 1171, 2127
BslI CCNNNNNNNGG 5 cut(s) 321, 1103, 1280, 1551, 2225
BsmAI GTCTC 6 cut(s) 26, 54, 362, 412, 432, 2276
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 3 cut(s) 637, 1171, 2127
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 4 cut(s) 1146, 1397, 1524, 1922
Bso31I GGTCTC 3 cut(s) 26, 54, 2276
BsoBI CYCGRG 2 cut(s) 365, 1437
Bsp119I TTCGAA 1 cut(s) 2077
Bsp120I GGGCCC 1 cut(s) 1395
Bsp1286I GDGCHC 7 cut(s) 192, 359, 532, 1290, 1399, 2247, 2305
Bsp13I TCCGGA 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 1930
Bsp1720I GCTNAGC 1 cut(s) 2198
BspACI CCGC 6 cut(s) 81, 807, 1240, 1399, 2262, 2348
BspANI GGCC 4 cut(s) 1146, 1397, 1524, 1922
BspCNI CTCAG 9 cut(s) 234, 261, 646, 877, 976, 1147, 2147, 2190, 2413
BspDI ATCGAT 1 cut(s) 618
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 2324
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 9 cut(s) 133, 358, 364, 754, 1397, 1921, 2266, 2352, 2406
BspMAI CTGCAG 3 cut(s) 541, 776, 2158
BspMI ACCTGC 1 cut(s) 499
BspPI GGATC 6 cut(s) 461, 938, 2281, 2387, 2399, 2412
BspT104I TTCGAA 1 cut(s) 2077
BspTI CTTAAG 1 cut(s) 1252
BspTNI GGTCTC 3 cut(s) 26, 54, 2276
BsrDI GCAATG 3 cut(s) 1199, 1512, 2202
BsrGI TGTACA 1 cut(s) 1930
BsrI ACTGG 6 cut(s) 772, 999, 1588, 2104, 2263, 2268
BssECI CCNNGG 8 cut(s) 504, 1097, 1098, 1134, 1293, 1914, 2308, 2354
BssNAI GTATAC 1 cut(s) 1975
BssT1I CCWWGG 5 cut(s) 504, 1134, 1293, 1914, 2308
Bst1107I GTATAC 1 cut(s) 1975
Bst2UI CCWGG 3 cut(s) 164, 1099, 1489
Bst4CI ACNGT 3 cut(s) 37, 382, 1565
Bst6I CTCTTC 2 cut(s) 1725, 1763
BstAFI CTTAAG 1 cut(s) 1252
BstAPI GCANNNNNTGC 2 cut(s) 880, 2150
BstAUI TGTACA 1 cut(s) 1930
BstBAI YACGTR 1 cut(s) 1222
BstBI TTCGAA 1 cut(s) 2077
BstC8I GCNNGC 9 cut(s) 192, 276, 528, 600, 1290, 1399, 1779, 2146, 2305
BstF5I GGATG 4 cut(s) 82, 790, 1648, 1831
BstFNI CGCG 1 cut(s) 2324
BstHHI GCGC 3 cut(s) 660, 1617, 2324
BstMAI GTCTC 6 cut(s) 26, 54, 362, 412, 432, 2276
BstNI CCWGG 3 cut(s) 164, 1099, 1489
BstNSI RCATGY 2 cut(s) 554, 2452
BstSCI CCNGG 3 cut(s) 162, 1097, 1487
BstSFI CTRYAG 6 cut(s) 378, 537, 772, 993, 1561, 2154
BstSLI GKGCMC 2 cut(s) 1399, 2247
BstUI CGCG 1 cut(s) 2324
BstV1I GCAGC 8 cut(s) 551, 758, 821, 1181, 1420, 2140, 2168, 2353
BstV2I GAAGAC 2 cut(s) 150, 1267
BstX2I RGATCY 3 cut(s) 943, 2273, 2404
BstYI RGATCY 3 cut(s) 943, 2273, 2404
BstZ17I GTATAC 1 cut(s) 1975
Bsu15I ATCGAT 1 cut(s) 618
BsuI GTATCC 1 cut(s) 1309
BsuRI GGCC 4 cut(s) 1146, 1397, 1524, 1922
BsuTUI ATCGAT 1 cut(s) 618
BtsCI GGATG 4 cut(s) 82, 790, 1648, 1831
BtsI GCAGTG 2 cut(s) 781, 1989
BtsIMutI CAGTG 9 cut(s) 765, 781, 1474, 1581, 1989, 2056, 2097, 2247, 2275
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 9 cut(s) 192, 276, 528, 600, 1290, 1399, 1779, 2146, 2305
CaiI CAGNNNCTG 1 cut(s) 241
CciI TCATGA 1 cut(s) 909
CfoI GCGC 3 cut(s) 660, 1617, 2324
Cfr13I GGNCC 7 cut(s) 362, 1395, 1396, 1522, 1542, 1704, 1920
ClaI ATCGAT 1 cut(s) 618
Csp6I GTAC 2 cut(s) 1219, 1931
CviQI GTAC 2 cut(s) 1219, 1931
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 2 cut(s) 1725, 1763
EarI CTCTTC 2 cut(s) 1725, 1763
EciI GGCGGA 1 cut(s) 2277
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 5 cut(s) 504, 1134, 1293, 1914, 2308
Eco24I GRGCYC 3 cut(s) 192, 359, 1399
Eco31I GGTCTC 3 cut(s) 26, 54, 2276
Eco47I GGWCC 3 cut(s) 362, 1542, 1704
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 6 cut(s) 579, 1481, 1749, 1767, 2032, 2105
Eco88I CYCGRG 2 cut(s) 365, 1437
EcoICRI GAGCTC 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 1741
EcoRII CCWGG 3 cut(s) 162, 1097, 1487
EcoT14I CCWWGG 5 cut(s) 504, 1134, 1293, 1914, 2308
EcoT38I GRGCYC 3 cut(s) 192, 359, 1399
ErhI CCWWGG 5 cut(s) 504, 1134, 1293, 1914, 2308
Esp3I CGTCTC 1 cut(s) 362
FaqI GGGAC 3 cut(s) 637, 1171, 2127
FauI CCCGC 2 cut(s) 1247, 1406
FauNDI CATATG 1 cut(s) 2122
FbaI TGATCA 2 cut(s) 778, 2251
FblI GTMKAC 1 cut(s) 1974
FokI GGATG 4 cut(s) 89, 797, 1655, 1838
FriOI GRGCYC 3 cut(s) 192, 359, 1399
FspBI CTAG 7 cut(s) 1077, 1403, 1493, 1958, 2117, 2186, 2298
GlaI GCGC 3 cut(s) 659, 1616, 2323
GsaI CCCAGC 2 cut(s) 157, 1192
GsuI CTGGAG 4 cut(s) 88, 185, 960, 1791
HaeIII GGCC 4 cut(s) 1146, 1397, 1524, 1922
HapII CCGG 3 cut(s) 129, 360, 1578
HhaI GCGC 3 cut(s) 660, 1617, 2324
Hin6I GCGC 3 cut(s) 658, 1615, 2322
HinP1I GCGC 3 cut(s) 658, 1615, 2322
HincII GTYRAC 2 cut(s) 309, 763
HindII GTYRAC 2 cut(s) 309, 763
HindIII AAGCTT 3 cut(s) 389, 579, 1779
HinfI GANTC 9 cut(s) 113, 119, 125, 169, 238, 405, 482, 1517, 1715
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 3 cut(s) 129, 360, 1578
HphI GGTGA 4 cut(s) 480, 1228, 1338, 2291
Hpy166II GTNNAC 6 cut(s) 309, 763, 1457, 1975, 2097, 2128
Hpy8I GTNNAC 6 cut(s) 309, 763, 1457, 1975, 2097, 2128
HpyAV CCTTC 6 cut(s) 554, 1039, 1274, 1852, 1889, 2080
HpyCH4III ACNGT 3 cut(s) 37, 382, 1565
HpyCH4IV ACGT 6 cut(s) 371, 679, 1068, 1221, 1378, 1663
HpySE526I ACGT 6 cut(s) 371, 679, 1068, 1221, 1378, 1663
HspAI GCGC 3 cut(s) 658, 1615, 2322
Kpn2I TCCGGA 1 cut(s) 359
Ksp22I TGATCA 2 cut(s) 778, 2251
KspAI GTTAAC 1 cut(s) 309
LmnI GCTCC 6 cut(s) 113, 137, 362, 1138, 1678, 2264
Lsp1109I GCAGC 8 cut(s) 551, 758, 821, 1181, 1420, 2140, 2168, 2353
LweI GCATC 6 cut(s) 67, 205, 1340, 1547, 1816, 2131
MaeI CTAG 7 cut(s) 1077, 1403, 1493, 1958, 2117, 2186, 2298
MaeII ACGT 6 cut(s) 371, 679, 1068, 1221, 1378, 1663
MaeIII GTNAC 2 cut(s) 37, 1374
MflI RGATCY 3 cut(s) 943, 2273, 2404
MhlI GDGCHC 7 cut(s) 192, 359, 532, 1290, 1399, 2247, 2305
MlsI TGGCCA 1 cut(s) 1146
MluNI TGGCCA 1 cut(s) 1146
MlyI GAGTC 2 cut(s) 119, 414
MmeI TCCRAC 7 cut(s) 147, 231, 700, 1084, 1620, 2038, 2357
Mox20I TGGCCA 1 cut(s) 1146
MroI TCCGGA 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 2034
MscI TGGCCA 1 cut(s) 1146
MseI TTAA 7 cut(s) 308, 446, 702, 1253, 1700, 2015, 2328
MslI CAYNNNNRTG 1 cut(s) 1530
Msp20I TGGCCA 1 cut(s) 1146
MspA1I CMGCKG 3 cut(s) 83, 542, 1172
MspCI CTTAAG 1 cut(s) 1252
MspI CCGG 3 cut(s) 129, 360, 1578
MspR9I CCNGG 3 cut(s) 164, 1099, 1489
Mva1269I GAATGC 1 cut(s) 660
MvaI CCWGG 3 cut(s) 164, 1099, 1489
MvnI CGCG 1 cut(s) 2324
NdeI CATATG 1 cut(s) 2122
NlaIV GGNNCC 9 cut(s) 133, 358, 364, 754, 1397, 1921, 2266, 2352, 2406
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 2 cut(s) 554, 2452
NspV TTCGAA 1 cut(s) 2077
PagI TCATGA 1 cut(s) 909
PaqCI CACCTGC 1 cut(s) 499
PasI CCCWGGG 1 cut(s) 1098
PciI ACATGT 1 cut(s) 2448
PctI GAATGC 1 cut(s) 660
PdmI GAANNNNTTC 1 cut(s) 2034
PfeI GAWTC 7 cut(s) 113, 119, 169, 238, 482, 1517, 1715
PflMI CCANNNNNTGG 1 cut(s) 2225
PleI GAGTC 2 cut(s) 119, 413
PpsI GAGTC 2 cut(s) 119, 413
Ppu21I YACGTR 1 cut(s) 1222
PscI ACATGT 1 cut(s) 2448
PsiI TTATAA 2 cut(s) 837, 1061
Psp124BI GAGCTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 1068
Psp6I CCWGG 3 cut(s) 162, 1097, 1487
PspFI CCCAGC 2 cut(s) 153, 1188
PspGI CCWGG 3 cut(s) 162, 1097, 1487
PspN4I GGNNCC 9 cut(s) 133, 358, 364, 754, 1397, 1921, 2266, 2352, 2406
PspOMI GGGCCC 1 cut(s) 1395
PspPI GGNCC 7 cut(s) 362, 1395, 1396, 1522, 1542, 1704, 1920
PstI CTGCAG 3 cut(s) 541, 776, 2158
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 3 cut(s) 943, 2273, 2404
PvuII CAGCTG 2 cut(s) 542, 1172
RsaI GTAC 2 cut(s) 1220, 1932
RsaNI GTAC 2 cut(s) 1219, 1931
RseI CAYNNNNRTG 1 cut(s) 1530
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 7 cut(s) 308, 446, 702, 1253, 1700, 2015, 2328
Sau96I GGNCC 7 cut(s) 362, 1395, 1396, 1522, 1542, 1704, 1920
SchI GAGTC 2 cut(s) 119, 414
ScrFI CCNGG 3 cut(s) 164, 1099, 1489
SduI GDGCHC 7 cut(s) 192, 359, 532, 1290, 1399, 2247, 2305
SfaNI GCATC 6 cut(s) 67, 205, 1340, 1547, 1816, 2131
SfcI CTRYAG 6 cut(s) 378, 537, 772, 993, 1561, 2154
SfuI TTCGAA 1 cut(s) 2077
SinI GGWCC 3 cut(s) 362, 1542, 1704
SmiMI CAYNNNNRTG 1 cut(s) 1530
SmlI CTYRAG 1 cut(s) 1252
SmoI CTYRAG 1 cut(s) 1252
SsiI CCGC 6 cut(s) 81, 807, 1240, 1399, 2262, 2348
SspMI CTAG 7 cut(s) 1077, 1403, 1493, 1958, 2117, 2186, 2298
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 162, 1097, 1487
StyI CCWWGG 5 cut(s) 504, 1134, 1293, 1914, 2308
TaaI ACNGT 3 cut(s) 37, 382, 1565
TaiI ACGT 6 cut(s) 374, 682, 1071, 1224, 1381, 1666
TaqI TCGA 5 cut(s) 180, 457, 618, 1650, 2077
TaqII GACCGA 1 cut(s) 1530
TatI WGTACW 1 cut(s) 1930
TauI GCSGC 2 cut(s) 83, 809
TfiI GAWTC 7 cut(s) 113, 119, 169, 238, 482, 1517, 1715
Tru1I TTAA 7 cut(s) 308, 446, 702, 1253, 1700, 2015, 2328
Tru9I TTAA 7 cut(s) 308, 446, 702, 1253, 1700, 2015, 2328
TscAI CASTG 9 cut(s) 772, 781, 1474, 1588, 1996, 2056, 2104, 2254, 2275
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 8 cut(s) 539, 771, 809, 1169, 1433, 2153, 2156, 2366
Tsp45I GTSAC 1 cut(s) 37
TspGWI ACGGA 2 cut(s) 72, 459
TspRI CASTG 9 cut(s) 772, 781, 1474, 1588, 1996, 2056, 2104, 2254, 2275
Van91I CCANNNNNTGG 1 cut(s) 2225
Vha464I CTTAAG 1 cut(s) 1252
VpaK11BI GGWCC 3 cut(s) 362, 1542, 1704
XapI RAATTY 7 cut(s) 448, 651, 831, 923, 1571, 1741, 2104
XbaI TCTAGA 1 cut(s) 1957
XceI RCATGY 2 cut(s) 554, 2452
XmiI GTMKAC 1 cut(s) 1974
XmnI GAANNNNTTC 1 cut(s) 2034
XspI CTAG 7 cut(s) 1077, 1403, 1493, 1958, 2117, 2186, 2298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.