MD15G1443600.v1.1

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004707
Physical Location & Seq
Reverse (-)
54383615 .. 54405229
21615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1443600.v1.1.491

Sequence Viewer

Length: 2478 bp
ATGGACGAAGAAACCCTAAAACCCCTTACTTCTGAAGAAGGCAACAATGGCGACCAACCCATGGCAGACCTCCCTGACCAAGACCTTAGCTCCTCCGATTCCGATTCCGACTCTGACTCTGGCTCTGAAGACGGCGACGACCAAGCCCACCATAACCTCCAACTCCAAACCCTTGAAGCCGAACTGTCTGCCAATCCTGGCAACTACGATACTCATGTCCAATACATCAAGATTCTGAGGCAAATGGCTAAAATTGAAAAGCTGAGGCAAGCAAGGGAAGCTATGAATCAGCTGTTTCCGTTAACCCCTTCTATGTGGCAGGATTGGGCTAAGGACGAAGCTTCTCTTTCCACTGGGTCTGATGCTTTTTGGGCTATTGAAAAGCTTTACGAGCGAGGAGTCTTTGAATATCTGTCCATCTCTCTTTGGTGTGACTACTTAGATTTTGTTAAAGAAAATGATCCCTCAGTAAGAGAATGTTCGCCTGCTGGTATTTCGAAGGCCAGAGATCTATTTGAACGTGCTCTTACTGCAGCTGGTTTGCATGTTTCTGAAGGCAATAAACTATGGGAAGCCTATCGGTCATTTGAGCAAGCTATATTTCATACTATCGACGAGAAAGACAATCAGGCAAGAGAAAAGCAAATTCAGCGTGTCCGCTCTTTATTCCACCGTCAGTTGTCAGTCCCCCATGTTAATATGAGGTCAACACTTTTGGATTACAAGGCATGGGAGGTGGAACAAGGAAACATTCTTGATGCTGGCTCCAGTGACCTGGATGGAATTTCATTCCAGGTTGCCTCAGCGTACAAAAAGGCACTGGAAATGTATGATGCTCGCATTCATCTTGAAGAACAGATTTGTCTGCAGGATATGTCAGAGTCGGAAAGACTTCAAAATTTCATGAACTACTTGAGATTTGAACAGTCTTCTGGAGATCCAGCCCGGGTCCAGACGTTGTATGAACGTGCTATTACGGCCTTTCCTATATCCAGTTATCTCTGGCTTGATTATACTCGTTATTTGGACAAAACATTGAAGGTTGGCAATGTTATAACCAACGTTTATTCCAGGGCTGTAATGAATTGCCCCTGTGTTGGAGAGCTTTGGGTCCAATACATGCTTTCCTTGGAGCGTGGTCATGTTTCTGAGAAAGAGATTGCCTCTGTGTTTGAGAAGTCTCTGCTATGCACTTTAACCCTTGATGAGTACCTAGATTTGTACCTCACCCGTGTAGATGGCTTAAGAAGAAGAATATCATGCGCTGTTGAAAGAGAGCATGGCTTGGAGTATTCATTAATCAGGGAAACCTTTCAGAATGCATCAGATTACTTGTCAGCATACCTGAAGAACACGGATGGCTTGCTACGTTTGCATGCTTATTGGGCCCGTTTGGAGCTAAATCTACATAAGGACCAACTGGCAGCTCGTGGAGTTTGGGAGACTTTACTTAAGCTCTGTGGTTCAATGCTGGAAGCCTGGCAAGGTTATATAGCAATGGAAACTGAATTGGGTCATGTTAATGAAGCTCGGGCCCTATATAGGAGATGTTACAGTAAAAGATTTCCCAGAACTGGTTCAGAGGACATCTGCCACTCGTGGTTACGTTTTGAGAGGGAGTTTGGTTCGTTGGATGATTTTGACCATGCAGTACAGAAGGTCACTCCTCGTCTGGAAGAGCTAGAATTATTCAGGTCACAACAAGAATCCAAATTGACAGAAGAGTCTCCTTTGAAGAAGAATGCTCGTGATAAGAGAAAGCAAGTTTCTGAGATACCTAATGAACAGCATCCAGCAAAGAGGCAAAAAGATGCAGCCCAAAAGCCAAAGAAAGCACATGGGAAGGATTCTGAGGTGGTGAACTCAGTTGAACAAACTAAAGTGGAAAAGATCAAGGCCATGGGTGACCAACCAGATAGAGAAAATGAGCAACAGATGAAAGAGCGTGTTCCAGAGAAAGGAAAAGTATATGCTGACCAGTGTACTGTTTTTGTTTCAAATATTAACCTTAAGGCAAATGATGAACATCTGCGTCAATTCTTCAGTGATGTTGGGGGAGTGGTTGCTGTAAGAATACTACATGACAAGTTCACTGGAAAGTCAAGGGGATTGGCATATGTGGATTTCTCAGATGATGCCCACCTCGTTGCAGCTGTTGCAAAAAATAAGCAGATGCTCCTTGGGAAGAAGCTGAGCATTGCACGATCAGATCCAAAGCATGGGAAGAAGGAACAAGCCAGGGGAGCTGAGTCTAATGATTCTGCTGAGGCCTCCAATGCACCTAGAGCACGTCAAGGACAGCAGTCTACTTATAACCGAGGAAGAGATAATGTACAGCTGAAGGGTAGGAACACATTTGCAACTCCCAGGAATATCCTTGCACTTGGTCGTAGTACAAACAAACCAACTACTGAGAAACAGGATGATGAAAAGCCAAAATCCAATGATGAGTTCAGAAACATGTTCCTCAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

826

Amino Acids

94.39

Weight (kDa)

5.78

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 55 - 560 1.6e-33 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 58 - 241 3.7e-18 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 59 - 199 2.9e-06 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 294 - 361 4.9e-12 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 294 - 380 2.6e-08 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
RRM_1 PF00076 663 - 731 7.9e-14 RNA recognition motif
Lsm_interact PF05391 808 - 824 6.9e-07 Lsm interaction motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1055, 2313
AasI GACNNNNNNGTC 1 cut(s) 1723
AccB7I CCANNNNNTGG 2 cut(s) 61, 2219
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 2306
AciI CCGC 1 cut(s) 658
AclI AACGTT 1 cut(s) 1062
AclWI GGATC 3 cut(s) 455, 932, 2204
AcsI RAATTY 3 cut(s) 645, 783, 898
AcuI CTGAAG 6 cut(s) 54, 147, 573, 1367, 2026, 2360
AfaI GTAC 7 cut(s) 809, 1211, 1223, 1653, 1984, 2334, 2395
AfiI CCNNNNNNNGG 6 cut(s) 61, 1097, 1542, 1574, 1958, 2219
AflII CTTAAG 3 cut(s) 1243, 1451, 2009
AflIII ACRYGT 1 cut(s) 2460
AjiI CACGTC 1 cut(s) 2291
AjnI CCWGG 7 cut(s) 196, 774, 792, 1070, 1478, 2237, 2366
AjuI GAANNNNNNNTTGG 2 cut(s) 2435, 2467
Alw21I GWGCWC 2 cut(s) 526, 2290
Alw26I GTCTC 3 cut(s) 1185, 1436, 1731
AlwI GGATC 3 cut(s) 455, 932, 2204
Ama87I CYCGRG 2 cut(s) 945, 1530
AoxI GGCC 6 cut(s) 501, 978, 1386, 1533, 1896, 2268
ApaI GGGCCC 2 cut(s) 1390, 1537
ApeKI GCWGC 4 cut(s) 533, 1424, 1814, 2150
ApoI RAATTY 3 cut(s) 645, 783, 898
AseI ATTAAT 1 cut(s) 1298
Asp700I GAANNNNTTC 3 cut(s) 891, 1311, 1576
AspLEI GCGC 1 cut(s) 1265
AspS9I GGNCC 7 cut(s) 949, 1111, 1386, 1387, 1414, 1533, 1534
AsuC2I CCSGG 2 cut(s) 946, 947
AsuHPI GGTGA 3 cut(s) 1219, 1870, 1916
AsuII TTCGAA 1 cut(s) 497
AvaI CYCGRG 2 cut(s) 945, 1530
AvaII GGWCC 3 cut(s) 949, 1111, 1414
BaeGI GKGCMC 2 cut(s) 1390, 1537
BaeI ACNNNNGTAYC 2 cut(s) 201, 234
BanII GRGCYC 2 cut(s) 1390, 1537
BauI CACGAG 3 cut(s) 1428, 1597, 1746
BbsI GAAGAC 2 cut(s) 135, 921
Bbv12I GWGCWC 2 cut(s) 526, 2290
BbvCI CCTCAGC 3 cut(s) 263, 802, 2265
BbvI GCAGC 4 cut(s) 545, 1436, 1826, 2162
BccI CCATC 4 cut(s) 425, 773, 1232, 1352
BceAI ACGGC 2 cut(s) 148, 993
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 7 cut(s) 198, 776, 794, 1072, 1480, 2239, 2368
BcnI CCSGG 2 cut(s) 946, 947
BcoDI GTCTC 3 cut(s) 1185, 1436, 1731
BfaI CTAG 3 cut(s) 1214, 1682, 2283
BfmI CTRYAG 2 cut(s) 531, 866
BfrI CTTAAG 3 cut(s) 1243, 1451, 2009
BglII AGATCT 1 cut(s) 508
BisI GCNGC 4 cut(s) 534, 1425, 1815, 2151
BlpI GCTNAGC 1 cut(s) 2192
BlsI GCNGC 4 cut(s) 535, 1426, 1816, 2152
Bme1390I CCNGG 9 cut(s) 198, 776, 794, 946, 947, 1072, 1480, 2239, 2368
Bme18I GGWCC 3 cut(s) 949, 1111, 1414
BmeT110I CYCGRG 2 cut(s) 945, 1530
BmgBI CACGTC 1 cut(s) 2291
BmgT120I GGNCC 7 cut(s) 949, 1111, 1386, 1387, 1414, 1533, 1534
BmiI GGNNCC 5 cut(s) 766, 950, 1112, 1388, 1535
BmrFI CCNGG 9 cut(s) 198, 776, 794, 946, 947, 1072, 1480, 2239, 2368
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 8 cut(s) 352, 748, 823, 1331, 1798, 1801, 2125, 2163
BmuI ACTGGG 1 cut(s) 363
BoxI GACNNNNGTC 1 cut(s) 2302
BpiI GAAGAC 2 cut(s) 135, 921
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 2 cut(s) 751, 954
Bpu10I CCTNAGC 5 cut(s) 86, 263, 330, 802, 2265
Bpu1102I GCTNAGC 1 cut(s) 2192
Bpu14I TTCGAA 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 2 cut(s) 946, 947
BsaBI GATNNNNATC 1 cut(s) 2025
BsaJI CCNNGG 9 cut(s) 60, 945, 1071, 1128, 1899, 2179, 2238, 2317, 2366
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 1097, 1542, 1574, 1958, 2219
Bse1I ACTGG 8 cut(s) 358, 768, 825, 993, 1425, 1579, 1978, 2098
Bse3DI GCAATG 3 cut(s) 1054, 1503, 2196
Bse8I GATNNNNATC 1 cut(s) 2025
BseBI CCWGG 7 cut(s) 198, 776, 794, 1072, 1480, 2239, 2368
BseDI CCNNGG 9 cut(s) 60, 945, 1071, 1128, 1899, 2179, 2238, 2317, 2366
BseGI GGATG 5 cut(s) 784, 1363, 1639, 1789, 2428
BseJI GATNNNNATC 1 cut(s) 2025
BseLI CCNNNNNNNGG 6 cut(s) 61, 1097, 1542, 1574, 1958, 2219
BseMI GCAATG 3 cut(s) 1054, 1503, 2196
BseNI ACTGG 8 cut(s) 358, 768, 825, 993, 1425, 1579, 1978, 2098
BseRI GAGGAG 3 cut(s) 82, 411, 1656
BseSI GKGCMC 2 cut(s) 1390, 1537
BseXI GCAGC 4 cut(s) 545, 1436, 1826, 2162
BshFI GGCC 6 cut(s) 503, 980, 1388, 1535, 1898, 2270
BsiHKAI GWGCWC 2 cut(s) 526, 2290
BsiHKCI CYCGRG 2 cut(s) 945, 1530
BsiSI CCGG 1 cut(s) 946
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 6 cut(s) 61, 1097, 1542, 1574, 1958, 2219
BsmAI GTCTC 3 cut(s) 1185, 1436, 1731
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 3 cut(s) 840, 1324, 1747
BsnI GGCC 6 cut(s) 503, 980, 1388, 1535, 1898, 2270
BsoBI CYCGRG 2 cut(s) 945, 1530
Bsp119I TTCGAA 1 cut(s) 497
Bsp120I GGGCCC 2 cut(s) 1386, 1533
Bsp1286I GDGCHC 4 cut(s) 526, 1390, 1537, 2290
Bsp1407I TGTACA 1 cut(s) 2332
Bsp143I GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
Bsp1720I GCTNAGC 1 cut(s) 2192
Bsp19I CCATGG 2 cut(s) 60, 1899
BspACI CCGC 1 cut(s) 658
BspANI GGCC 6 cut(s) 503, 980, 1388, 1535, 1898, 2270
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 5 cut(s) 766, 950, 1112, 1388, 1535
BspMAI CTGCAG 2 cut(s) 535, 870
BspPI GGATC 3 cut(s) 455, 932, 2204
BspQI GCTCTTC 1 cut(s) 1671
BspT104I TTCGAA 1 cut(s) 497
BspTI CTTAAG 3 cut(s) 1243, 1451, 2009
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 3 cut(s) 1054, 1503, 2196
BsrGI TGTACA 1 cut(s) 2332
BsrI ACTGG 8 cut(s) 358, 768, 825, 993, 1425, 1579, 1978, 2098
BssECI CCNNGG 9 cut(s) 60, 945, 1071, 1128, 1899, 2179, 2238, 2317, 2366
BssMI GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
BssSI CACGAG 3 cut(s) 1428, 1597, 1746
BssT1I CCWWGG 4 cut(s) 60, 1128, 1899, 2179
Bst2BI CACGAG 3 cut(s) 1428, 1597, 1746
Bst2UI CCWGG 7 cut(s) 198, 776, 794, 1072, 1480, 2239, 2368
Bst4CI ACNGT 5 cut(s) 186, 674, 927, 1556, 1987
Bst6I CTCTTC 3 cut(s) 1671, 1716, 2317
BstAFI CTTAAG 3 cut(s) 1243, 1451, 2009
BstAPI GCANNNNNTGC 1 cut(s) 2156
BstAUI TGTACA 1 cut(s) 2332
BstBI TTCGAA 1 cut(s) 497
BstC8I GCNNGC 7 cut(s) 270, 486, 594, 763, 838, 1364, 1377
BstDSI CCRYGG 2 cut(s) 60, 1899
BstEII GGTNACC 1 cut(s) 1904
BstF5I GGATG 5 cut(s) 784, 1363, 1639, 1789, 2428
BstHHI GCGC 1 cut(s) 1265
BstKTI GATC 6 cut(s) 463, 511, 940, 1893, 2207, 2212
BstMAI GTCTC 3 cut(s) 1185, 1436, 1731
BstMBI GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
BstNI CCWGG 7 cut(s) 198, 776, 794, 1072, 1480, 2239, 2368
BstNSI RCATGY 4 cut(s) 548, 1123, 1379, 2464
BstPAI GACNNNNGTC 1 cut(s) 2302
BstPI GGTNACC 1 cut(s) 1904
BstSCI CCNGG 9 cut(s) 196, 774, 792, 944, 945, 1070, 1478, 2237, 2366
BstSFI CTRYAG 2 cut(s) 531, 866
BstSLI GKGCMC 2 cut(s) 1390, 1537
BstV1I GCAGC 4 cut(s) 545, 1436, 1826, 2162
BstV2I GAAGAC 2 cut(s) 135, 921
BstX2I RGATCY 3 cut(s) 508, 937, 2209
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 3 cut(s) 508, 937, 2209
BsuRI GGCC 6 cut(s) 503, 980, 1388, 1535, 1898, 2270
BtgI CCRYGG 2 cut(s) 60, 1899
BtrI CACGTC 1 cut(s) 2291
BtsCI GGATG 5 cut(s) 784, 1363, 1639, 1789, 2428
BtsIMutI CAGTG 6 cut(s) 351, 775, 818, 1985, 2050, 2091
Cac8I GCNNGC 7 cut(s) 270, 486, 594, 763, 838, 1364, 1377
CciI TCATGA 1 cut(s) 903
CfoI GCGC 1 cut(s) 1265
Cfr13I GGNCC 7 cut(s) 949, 1111, 1386, 1387, 1414, 1533, 1534
Cfr9I CCCGGG 1 cut(s) 945
CseI GACGC 1 cut(s) 2021
Csp6I GTAC 7 cut(s) 808, 1210, 1222, 1652, 1983, 2333, 2394
CviQI GTAC 7 cut(s) 808, 1210, 1222, 1652, 1983, 2333, 2394
DpnI GATC 6 cut(s) 462, 510, 939, 1892, 2206, 2211
DpnII GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
DrdI GACNNNNNNGTC 1 cut(s) 1723
DseDI GACNNNNNNGTC 1 cut(s) 1723
Eam1104I CTCTTC 3 cut(s) 1671, 1716, 2317
EarI CTCTTC 3 cut(s) 1671, 1716, 2317
Eco130I CCWWGG 4 cut(s) 60, 1128, 1899, 2179
Eco147I AGGCCT 1 cut(s) 2270
Eco24I GRGCYC 2 cut(s) 1390, 1537
Eco47I GGWCC 3 cut(s) 949, 1111, 1414
Eco57I CTGAAG 6 cut(s) 54, 147, 573, 1367, 2026, 2360
Eco88I CYCGRG 2 cut(s) 945, 1530
Eco91I GGTNACC 1 cut(s) 1904
EcoO109I RGGNCCY 1 cut(s) 1534
EcoO65I GGTNACC 1 cut(s) 1904
EcoRII CCWGG 7 cut(s) 196, 774, 792, 1070, 1478, 2237, 2366
EcoT14I CCWWGG 4 cut(s) 60, 1128, 1899, 2179
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 2 cut(s) 1390, 1537
ErhI CCWWGG 4 cut(s) 60, 1128, 1899, 2179
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 1 cut(s) 671
FauNDI CATATG 1 cut(s) 2116
FblI GTMKAC 1 cut(s) 2306
Fnu4HI GCNGC 4 cut(s) 534, 1425, 1815, 2151
FokI GGATG 5 cut(s) 791, 1370, 1646, 1776, 2435
FriOI GRGCYC 2 cut(s) 1390, 1537
Fsp4HI GCNGC 4 cut(s) 534, 1425, 1815, 2151
FspBI CTAG 3 cut(s) 1214, 1682, 2283
GlaI GCGC 1 cut(s) 1264
GluI GCNGC 4 cut(s) 534, 1425, 1815, 2151
GsuI CTGGAG 2 cut(s) 751, 954
HaeIII GGCC 6 cut(s) 503, 980, 1388, 1535, 1898, 2270
HapII CCGG 1 cut(s) 946
HgaI GACGC 1 cut(s) 2021
HhaI GCGC 1 cut(s) 1265
Hin6I GCGC 1 cut(s) 1263
HinP1I GCGC 1 cut(s) 1263
HincII GTYRAC 2 cut(s) 303, 708
HindII GTYRAC 2 cut(s) 303, 708
HindIII AAGCTT 2 cut(s) 339, 383
HpaI GTTAAC 1 cut(s) 303
HpaII CCGG 1 cut(s) 946
HphI GGTGA 3 cut(s) 1219, 1870, 1916
Hpy166II GTNNAC 6 cut(s) 303, 708, 1861, 1983, 2091, 2307
Hpy188III TCNNGA 9 cut(s) 229, 755, 848, 904, 933, 952, 1673, 1748, 1952
Hpy8I GTNNAC 6 cut(s) 303, 708, 1861, 1983, 2091, 2307
Hpy99I CGWCG 2 cut(s) 140, 617
HpyAV CCTTC 9 cut(s) 32, 318, 493, 548, 1033, 1651, 1837, 2221, 2335
HpyCH4III ACNGT 5 cut(s) 186, 674, 927, 1556, 1987
HpyCH4IV ACGT 7 cut(s) 520, 956, 967, 1062, 1369, 1606, 2290
HpySE526I ACGT 7 cut(s) 520, 956, 967, 1062, 1369, 1606, 2290
HspAI GCGC 1 cut(s) 1263
KspAI GTTAAC 1 cut(s) 303
Kzo9I GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
LguI GCTCTTC 1 cut(s) 1671
LmnI GCTCC 6 cut(s) 95, 770, 1132, 1396, 2181, 2243
Lsp1109I GCAGC 4 cut(s) 545, 1436, 1826, 2162
LweI GCATC 8 cut(s) 352, 748, 823, 1331, 1798, 1801, 2125, 2163
MaeI CTAG 3 cut(s) 1214, 1682, 2283
MaeII ACGT 7 cut(s) 520, 956, 967, 1062, 1369, 1606, 2290
MaeIII GTNAC 7 cut(s) 431, 770, 1550, 1602, 1660, 1695, 1904
MalI GATC 6 cut(s) 462, 510, 939, 1892, 2206, 2211
MbiI CCGCTC 1 cut(s) 660
MboI GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
MflI RGATCY 3 cut(s) 508, 937, 2209
MhlI GDGCHC 4 cut(s) 526, 1390, 1537, 2290
MluCI AATT 9 cut(s) 252, 645, 783, 898, 1084, 1508, 1685, 1712, 2036
MlyI GAGTC 6 cut(s) 104, 110, 408, 890, 1733, 2258
MmeI TCCRAC 5 cut(s) 132, 184, 864, 1078, 1611
Mph1103I ATGCAT 1 cut(s) 1324
MroXI GAANNNNTTC 3 cut(s) 891, 1311, 1576
MslI CAYNNNNRTG 1 cut(s) 1521
MspA1I CMGCKG 4 cut(s) 292, 536, 2153, 2338
MspCI CTTAAG 3 cut(s) 1243, 1451, 2009
MspI CCGG 1 cut(s) 946
MspR9I CCNGG 9 cut(s) 198, 776, 794, 946, 947, 1072, 1480, 2239, 2368
Mva1269I GAATGC 3 cut(s) 840, 1324, 1747
MvaI CCWGG 7 cut(s) 198, 776, 794, 1072, 1480, 2239, 2368
NciI CCSGG 2 cut(s) 946, 947
NcoI CCATGG 2 cut(s) 60, 1899
NdeI CATATG 1 cut(s) 2116
NdeII GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
NlaIV GGNNCC 5 cut(s) 766, 950, 1112, 1388, 1535
NmuCI GTSAC 5 cut(s) 431, 770, 1660, 1695, 1904
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 4 cut(s) 548, 1123, 1379, 2464
NspV TTCGAA 1 cut(s) 497
PaeI GCATGC 1 cut(s) 1379
PagI TCATGA 1 cut(s) 903
PceI AGGCCT 1 cut(s) 2270
PciI ACATGT 1 cut(s) 2460
PciSI GCTCTTC 1 cut(s) 1671
PctI GAATGC 3 cut(s) 840, 1324, 1747
PdmI GAANNNNTTC 3 cut(s) 891, 1311, 1576
PfeI GAWTC 7 cut(s) 98, 104, 232, 286, 1706, 1847, 2258
PflMI CCANNNNNTGG 2 cut(s) 61, 2219
PkrI GCNGC 4 cut(s) 535, 1426, 1816, 2152
PleI GAGTC 6 cut(s) 104, 110, 407, 889, 1732, 2257
PpsI GAGTC 6 cut(s) 104, 110, 407, 889, 1732, 2257
PscI ACATGT 1 cut(s) 2460
PshAI GACNNNNGTC 1 cut(s) 2302
PshBI ATTAAT 1 cut(s) 1298
PsiI TTATAA 2 cut(s) 1055, 2313
Psp1406I AACGTT 1 cut(s) 1062
Psp6I CCWGG 7 cut(s) 196, 774, 792, 1070, 1478, 2237, 2366
PspEI GGTNACC 1 cut(s) 1904
PspGI CCWGG 7 cut(s) 196, 774, 792, 1070, 1478, 2237, 2366
PspN4I GGNNCC 5 cut(s) 766, 950, 1112, 1388, 1535
PspOMI GGGCCC 2 cut(s) 1386, 1533
PspPI GGNCC 7 cut(s) 949, 1111, 1386, 1387, 1414, 1533, 1534
PstI CTGCAG 2 cut(s) 535, 870
PsuI RGATCY 3 cut(s) 508, 937, 2209
PvuII CAGCTG 4 cut(s) 292, 536, 2153, 2338
RsaI GTAC 7 cut(s) 809, 1211, 1223, 1653, 1984, 2334, 2395
RsaNI GTAC 7 cut(s) 808, 1210, 1222, 1652, 1983, 2333, 2394
RseI CAYNNNNRTG 1 cut(s) 1521
SapI GCTCTTC 1 cut(s) 1671
SatI GCNGC 4 cut(s) 534, 1425, 1815, 2151
Sau3AI GATC 6 cut(s) 460, 508, 937, 1890, 2204, 2209
Sau96I GGNCC 7 cut(s) 949, 1111, 1386, 1387, 1414, 1533, 1534
SchI GAGTC 6 cut(s) 104, 110, 408, 890, 1733, 2258
ScrFI CCNGG 9 cut(s) 198, 776, 794, 946, 947, 1072, 1480, 2239, 2368
SduI GDGCHC 4 cut(s) 526, 1390, 1537, 2290
SfaNI GCATC 8 cut(s) 352, 748, 823, 1331, 1798, 1801, 2125, 2163
SfcI CTRYAG 2 cut(s) 531, 866
SfuI TTCGAA 1 cut(s) 497
SinI GGWCC 3 cut(s) 949, 1111, 1414
SmaI CCCGGG 1 cut(s) 947
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 4 cut(s) 913, 1243, 1451, 2009
SmoI CTYRAG 4 cut(s) 913, 1243, 1451, 2009
SphI GCATGC 1 cut(s) 1379
Sse9I AATT 9 cut(s) 252, 645, 783, 898, 1084, 1508, 1685, 1712, 2036
SseBI AGGCCT 1 cut(s) 2270
SsiI CCGC 1 cut(s) 658
SspI AATATT 1 cut(s) 2002
SspMI CTAG 3 cut(s) 1214, 1682, 2283
StuI AGGCCT 1 cut(s) 2270
StyD4I CCNGG 9 cut(s) 196, 774, 792, 944, 945, 1070, 1478, 2237, 2366
StyI CCWWGG 4 cut(s) 60, 1128, 1899, 2179
TaaI ACNGT 5 cut(s) 186, 674, 927, 1556, 1987
TaiI ACGT 7 cut(s) 523, 959, 970, 1065, 1372, 1609, 2293
TaqI TCGA 2 cut(s) 497, 612
TaqII GACCGA 1 cut(s) 570
TasI AATT 9 cut(s) 252, 645, 783, 898, 1084, 1508, 1685, 1712, 2036
TatI WGTACW 4 cut(s) 1651, 1982, 2332, 2393
TfiI GAWTC 7 cut(s) 98, 104, 232, 286, 1706, 1847, 2258
TscAI CASTG 6 cut(s) 358, 775, 825, 1985, 2050, 2098
TseFI GTSAC 5 cut(s) 431, 770, 1660, 1695, 1904
TseI GCWGC 4 cut(s) 533, 1424, 1814, 2150
Tsp45I GTSAC 5 cut(s) 431, 770, 1660, 1695, 1904
TspGWI ACGGA 2 cut(s) 288, 1370
TspMI CCCGGG 1 cut(s) 945
TspRI CASTG 6 cut(s) 358, 775, 825, 1985, 2050, 2098
Van91I CCANNNNNTGG 2 cut(s) 61, 2219
Vha464I CTTAAG 3 cut(s) 1243, 1451, 2009
VpaK11BI GGWCC 3 cut(s) 949, 1111, 1414
VspI ATTAAT 1 cut(s) 1298
XapI RAATTY 3 cut(s) 645, 783, 898
XceI RCATGY 4 cut(s) 548, 1123, 1379, 2464
XmaI CCCGGG 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 2306
XmnI GAANNNNTTC 3 cut(s) 891, 1311, 1576
XspI CTAG 3 cut(s) 1214, 1682, 2283
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.