Rroxscaffold_3G00240230

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004707
Physical Location & Seq
Reverse (-)
31907217 .. 31915805
8589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240230.1

Sequence Viewer

Length: 2541 bp
ATGGAGGAAGAAACCCTAAATCCTGAACCAGAGACCGTGACTTCGCAAGAGAACAACAATGGAGACCAACCCATGCTGGATGCCACTGACCAAAATCCTAACTCCTCCAGCTCCGATTCCGATTCCGACTCCGACTCCGGCTCCGATGACGAAGACCAAGCCCAGCAAAACCTGGAGATTCAAACACTCGAAGCCGAGCTCGCAACCAACCCCTCCAACTACGATGCCCATGTCCAATACATTAAGATTCTGAGGAAAATGGCTGACATTGAAAAGCTGAGGCGAGCGAGGGAAGCTATGAGTAAGCTGTTGCCGTTAACCCCTCCATGTGGCAGGATTGGGCCAAGGACGAACTTGCTATGGGCTCCGGACCCGAGACGTTTTCTACAGTTGAAAAGCTTTATGAGCAAGGAGTCTCTGATTATCTGGTTGTCCATCTCTCTTTGGCGAGACTACTTAAATTTTGTTCGAGAAAATGATCCGTCCGTAAAAGAATCTTCACCTGCTGGTATTTCCAAGGAAAGAGATTTATTTGAGCATGCTCTTACTGCAGCTGGTTTGCATGTATCTGAAGGCAATAAGATATGGGAAGCTTACAGGGAGTTTGAGCAAGCTATATGTGATACTATTGATGGGACTGACACTCAGGCAAGAGAGAAGCAAATTCAGCGCATTCGTGTTATATTCCAACGTCAGCTATCAGTTCCCCATGTTAATATGAGATCAACACTTTTGGCTTACAAGGCATGGGAGATGGAACAAGGAACCATTGTTGACACTGGCTGCAGTGATCAGGATTGTATTTCATCCCATGTTGCCGCAGCATACCAAAAGGCATTGGAAATTTATAATGCCCGTGTTCATTTTGAAGAACAAATCAGTCAGCATGATATGCCTGAGATAGAAAGACTTGAAAAGTTCATGAACTACTTGAAATTTGAGCAATCTTCTGGAGATCCTGCCCGTGTTCAAGTGTTGTATGAACGGGCTGTTGCTGAGTTTCCTATAGCCAGTCAGCTCTGGCTTGATTATACTTCCTATTTGGACAAAACTTTGAAGGTTGGCAGAATTATAAGCAACGTCTATTCTAGGGCTGTAAAGAATTGCCCCTGGGTTGGAGAACTTTGGGTCAGATATTTGCTTTCCTTGGAGCGTGGCCATGCTTCTGAGAAAGAGATTGCAGCTGTGTTTTACAAGTCCCAGCAATGCACCTTTTCAACCCTTGATGAGTACGTGGAGTTGTTTCTCACCCGCATAGATGGCTTAAGAAGAAGACTATCATGCCCCGTTGAAGGAGAGCACGCCTTGGATTATTCATTGTTGAGGGATACATTTCAGGTTCTCAGGCTAGATTCTGAAACCATTAAATCATCTAACAATTTGCATTTTCTAGATAGTATTGTTTGTGCTAAGGTTACTTGGGAGGGAGTCTGCATCGGATTACCTATCACCACAGATGAAGAACACAGATGTCCTGTTACTGGTACGATGATGGAAGCCTGGCTAGGTTATATAGCAATGGAAATTGAATCGGGCCATGTGAATGAAGCTCGTTCCATATATAGGAGATGCTACAGTAAAAAATTTCCCGGCACTGGTTCAGAGGACATTTGCTACTTGTGGTTGCGCTTTGAGAGGGAGTTTGGGTCGTTGGATGATTTCGACTATGCAGAACGTAAGGTTGCTCCCCGTCTGCAAGAGCTTCAACTATTAAGGTCCAAGCAAGAATCCAAATTGACTGAAGAGAGGGAGAATTCTCTCAAAAAGAATGTGCGTGAGAAGAGAAAGCAAGCTTCAGATGTAAGTGATGAACAACCTCCAGCAAAGAGGCAAAAGGATGCAACTCAAAAGCAAAAGAAAGTGCATGGGAAGGATTATGAGGTGCAGAACAAAGCTGAACAAAATGAAGGGAAAGAGATGAAAGCCAAGGGGGCCAAAGATGTACAAAATGAGATGAAAGAGCCTGTTCTAGAGAAAACAAAAGCAAATAATGAACATCTTCATCAGTTCTTCAGTGATGTTGGAGGAGTTGTTGCTATTCGAATACTGAAGGACAAGTTCACTGGAAATTCAAGGGGACTAGCATATGTGGACTTCTCAGATGATGCACACCTTGCTGCAGCCATAGCAAAAAATAAACAGACACTCCTAGACAGGAAGCTGAGCATTGCACGATCAAATCCAAAGCGTGGCAAGAAAGAACATGGTAGGCCAAATGAGTTTTATAATTTCTTTCTATGCACATTTCGTGTTCATCTGTGTAGAACCATGCTGATATGGGAACAGAAAAGCCCTTATCTCTTATATCATTTTCCTGGGCACACTGATCAAACTGGCACAGCCAGTGGATCTGGTGAGACCCCCAATGAATCTAGAGCAAGCCAAGGAAGTGACAGCGCGATTAAGAGGAAGAACACATTTGCGGTTCCGAGGAATGTTGCTGCACTTGGTTGGATCACGAATAAGCCAAAAACTCAGGATCCAGATGATGAAAAGCCAAAATCTAATGATGAGTTCAGAAACATGTTCCTGAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

846

Amino Acids

96.84

Weight (kDa)

5.96

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 60 - 397 1.5e-21 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 65 - 246 1e-08 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 285 - 367 7.1e-12 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 287 - 383 1.5e-07 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 308 - 415 6.2e-07 PRP39 N-terminal HAT repeat
RRM_1 PF00076 662 - 721 2.8e-11 RNA recognition motif
Lsm_interact PF05391 828 - 845 1.7e-07 Lsm interaction motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 849, 1073, 2226
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 511
AccB7I CCANNNNNTGG 1 cut(s) 2189
AccII CGCG 1 cut(s) 2399
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 3 cut(s) 819, 1252, 2423
AclWI GGATC 6 cut(s) 473, 950, 2356, 2462, 2474, 2487
AcoI YGGCCR 1 cut(s) 1156
AcsI RAATTY 7 cut(s) 460, 663, 843, 935, 1583, 1753, 2068
AcuI CTGAAG 5 cut(s) 591, 1761, 1779, 1996, 2069
AfaI GTAC 3 cut(s) 1232, 1486, 1944
AfiI CCNNNNNNNGG 7 cut(s) 329, 1115, 1292, 1481, 1563, 1595, 2189
AflII CTTAAG 1 cut(s) 1264
AflIII ACRYGT 1 cut(s) 2523
AjnI CCWGG 4 cut(s) 171, 1109, 1499, 2314
Alw21I GWGCWC 2 cut(s) 201, 1302
Alw26I GTCTC 6 cut(s) 26, 57, 370, 420, 444, 2351
AlwI GGATC 6 cut(s) 473, 950, 2356, 2462, 2474, 2487
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 5 cut(s) 341, 1156, 1534, 1932, 2210
ApeKI GCWGC 7 cut(s) 551, 783, 821, 1181, 2117, 2120, 2441
ApoI RAATTY 7 cut(s) 460, 663, 843, 935, 1583, 1753, 2068
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 1998
AspLEI GCGC 3 cut(s) 672, 1629, 2399
AspS9I GGNCC 5 cut(s) 341, 370, 1534, 1716, 1932
AsuC2I CCSGG 1 cut(s) 1590
AsuHPI GGTGA 4 cut(s) 492, 1240, 1441, 2366
AsuII TTCGAA 1 cut(s) 2041
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 2 cut(s) 370, 1716
BaeGI GKGCMC 1 cut(s) 2322
BalI TGGCCA 1 cut(s) 1158
BamHI GGATCC 1 cut(s) 2479
BanII GRGCYC 2 cut(s) 201, 367
BbsI GAAGAC 2 cut(s) 159, 1279
Bbv12I GWGCWC 2 cut(s) 201, 1302
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 7 cut(s) 563, 770, 833, 1193, 2104, 2132, 2428
BccI CCATC 5 cut(s) 443, 626, 748, 1253, 1486
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 4 cut(s) 173, 1111, 1501, 2316
BciVI GTATCC 1 cut(s) 1321
BclI TGATCA 2 cut(s) 790, 2326
BcnI CCSGG 1 cut(s) 1590
BcoDI GTCTC 6 cut(s) 26, 57, 370, 420, 444, 2351
BfaI CTAG 8 cut(s) 1089, 1349, 1391, 1505, 1970, 2081, 2150, 2373
BfmI CTRYAG 6 cut(s) 386, 549, 784, 1005, 1573, 2118
BfrI CTTAAG 1 cut(s) 1264
BfuAI ACCTGC 1 cut(s) 511
BfuI GTATCC 1 cut(s) 1321
BglI GCCNNNNNGGC 1 cut(s) 1931
BisI GCNGC 8 cut(s) 552, 784, 819, 822, 1182, 2118, 2121, 2442
BlpI GCTNAGC 1 cut(s) 2162
BlsI GCNGC 8 cut(s) 553, 785, 820, 823, 1183, 2119, 2122, 2443
Bme1390I CCNGG 5 cut(s) 173, 1111, 1501, 1590, 2316
Bme18I GGWCC 2 cut(s) 370, 1716
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 5 cut(s) 341, 370, 1534, 1716, 1932
BmiI GGNNCC 7 cut(s) 142, 366, 372, 766, 1933, 2427, 2481
BmrFI CCNGG 5 cut(s) 173, 1111, 1501, 1590, 2316
BmsI GCATC 6 cut(s) 70, 214, 1443, 1559, 1828, 2095
BpiI GAAGAC 2 cut(s) 159, 1279
BpmI CTGGAG 4 cut(s) 91, 194, 972, 1803
Bpu10I CCTNAGC 2 cut(s) 278, 1410
Bpu1102I GCTNAGC 1 cut(s) 2162
Bpu14I TTCGAA 1 cut(s) 2041
BpuMI CCSGG 1 cut(s) 1590
BsaAI YACGTR 1 cut(s) 1234
BsaI GGTCTC 3 cut(s) 26, 57, 2351
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 7 cut(s) 329, 1115, 1292, 1481, 1563, 1595, 2189
Bse1I ACTGG 7 cut(s) 784, 1011, 1486, 1600, 2068, 2338, 2343
Bse3DI GCAATG 3 cut(s) 1211, 1524, 2166
BseAI TCCGGA 1 cut(s) 367
BseBI CCWGG 4 cut(s) 173, 1111, 1501, 2316
BseGI GGATG 4 cut(s) 85, 806, 1660, 1843
BseLI CCNNNNNNNGG 7 cut(s) 329, 1115, 1292, 1481, 1563, 1595, 2189
BseMI GCAATG 3 cut(s) 1211, 1524, 2166
BseNI ACTGG 7 cut(s) 784, 1011, 1486, 1600, 2068, 2338, 2343
BseRI GAGGAG 2 cut(s) 94, 2040
BseSI GKGCMC 1 cut(s) 2322
BseXI GCAGC 7 cut(s) 563, 770, 833, 1193, 2104, 2132, 2428
BseYI CCCAGC 2 cut(s) 162, 1200
BsgI GTGCAG 2 cut(s) 1904, 2427
Bsh1236I CGCG 1 cut(s) 2399
BshFI GGCC 5 cut(s) 343, 1158, 1536, 1934, 2212
BsiHKAI GWGCWC 2 cut(s) 201, 1302
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 3 cut(s) 138, 368, 1590
BslFI GGGAC 3 cut(s) 649, 1183, 2091
BslI CCNNNNNNNGG 7 cut(s) 329, 1115, 1292, 1481, 1563, 1595, 2189
BsmAI GTCTC 6 cut(s) 26, 57, 370, 420, 444, 2351
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 3 cut(s) 649, 1183, 2091
BsmI GAATGC 1 cut(s) 672
BsnI GGCC 5 cut(s) 343, 1158, 1536, 1934, 2212
Bso31I GGTCTC 3 cut(s) 26, 57, 2351
BsoBI CYCGRG 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 2041
Bsp1286I GDGCHC 4 cut(s) 201, 367, 1302, 2322
Bsp13I TCCGGA 1 cut(s) 367
Bsp1407I TGTACA 1 cut(s) 1942
Bsp143I GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
Bsp1720I GCTNAGC 1 cut(s) 2162
BspACI CCGC 3 cut(s) 819, 1252, 2423
BspANI GGCC 5 cut(s) 343, 1158, 1536, 1934, 2212
BspEI TCCGGA 1 cut(s) 367
BspFNI CGCG 1 cut(s) 2399
BspHI TCATGA 1 cut(s) 921
BspLI GGNNCC 7 cut(s) 142, 366, 372, 766, 1933, 2427, 2481
BspMAI CTGCAG 3 cut(s) 553, 788, 2122
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 6 cut(s) 473, 950, 2356, 2462, 2474, 2487
BspT104I TTCGAA 1 cut(s) 2041
BspTI CTTAAG 1 cut(s) 1264
BspTNI GGTCTC 3 cut(s) 26, 57, 2351
BsrDI GCAATG 3 cut(s) 1211, 1524, 2166
BsrGI TGTACA 1 cut(s) 1942
BsrI ACTGG 7 cut(s) 784, 1011, 1486, 1600, 2068, 2338, 2343
BssMI GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
BssT1I CCWWGG 6 cut(s) 344, 516, 1146, 1305, 1926, 2383
Bst2UI CCWGG 4 cut(s) 173, 1111, 1501, 2316
Bst4CI ACNGT 3 cut(s) 37, 390, 1577
Bst6I CTCTTC 2 cut(s) 1737, 1775
BstAFI CTTAAG 1 cut(s) 1264
BstAPI GCANNNNNTGC 2 cut(s) 892, 2114
BstAUI TGTACA 1 cut(s) 1942
BstBAI YACGTR 1 cut(s) 1234
BstBI TTCGAA 1 cut(s) 2041
BstC8I GCNNGC 7 cut(s) 201, 285, 540, 612, 1302, 1791, 2380
BstF5I GGATG 4 cut(s) 85, 806, 1660, 1843
BstFNI CGCG 1 cut(s) 2399
BstHHI GCGC 3 cut(s) 672, 1629, 2399
BstKTI GATC 9 cut(s) 481, 725, 793, 958, 2177, 2329, 2351, 2457, 2482
BstMAI GTCTC 6 cut(s) 26, 57, 370, 420, 444, 2351
BstMBI GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
BstNI CCWGG 4 cut(s) 173, 1111, 1501, 2316
BstNSI RCATGY 3 cut(s) 542, 566, 2527
BstSCI CCNGG 5 cut(s) 171, 1109, 1499, 1588, 2314
BstSFI CTRYAG 6 cut(s) 386, 549, 784, 1005, 1573, 2118
BstSLI GKGCMC 1 cut(s) 2322
BstUI CGCG 1 cut(s) 2399
BstV1I GCAGC 7 cut(s) 563, 770, 833, 1193, 2104, 2132, 2428
BstV2I GAAGAC 2 cut(s) 159, 1279
BstX2I RGATCY 3 cut(s) 955, 2348, 2479
BstYI RGATCY 3 cut(s) 955, 2348, 2479
BsuI GTATCC 1 cut(s) 1321
BsuRI GGCC 5 cut(s) 343, 1158, 1536, 1934, 2212
BtsCI GGATG 4 cut(s) 85, 806, 1660, 1843
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 8 cut(s) 84, 777, 793, 1593, 2020, 2061, 2322, 2350
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 7 cut(s) 201, 285, 540, 612, 1302, 1791, 2380
CciI TCATGA 1 cut(s) 921
CfoI GCGC 3 cut(s) 672, 1629, 2399
Cfr13I GGNCC 5 cut(s) 341, 370, 1534, 1716, 1932
Csp6I GTAC 3 cut(s) 1231, 1485, 1943
CviQI GTAC 3 cut(s) 1231, 1485, 1943
DpnI GATC 9 cut(s) 480, 724, 792, 957, 2176, 2328, 2350, 2456, 2481
DpnII GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
EaeI YGGCCR 1 cut(s) 1156
Eam1104I CTCTTC 2 cut(s) 1737, 1775
EarI CTCTTC 2 cut(s) 1737, 1775
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 6 cut(s) 344, 516, 1146, 1305, 1926, 2383
Eco24I GRGCYC 2 cut(s) 201, 367
Eco31I GGTCTC 3 cut(s) 26, 57, 2351
Eco47I GGWCC 2 cut(s) 370, 1716
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 5 cut(s) 591, 1761, 1779, 1996, 2069
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 1753
EcoRII CCWGG 4 cut(s) 171, 1109, 1499, 2314
EcoT14I CCWWGG 6 cut(s) 344, 516, 1146, 1305, 1926, 2383
EcoT38I GRGCYC 2 cut(s) 201, 367
ErhI CCWWGG 6 cut(s) 344, 516, 1146, 1305, 1926, 2383
Esp3I CGTCTC 1 cut(s) 370
FaqI GGGAC 3 cut(s) 649, 1183, 2091
FauI CCCGC 1 cut(s) 1259
FauNDI CATATG 1 cut(s) 2086
FbaI TGATCA 2 cut(s) 790, 2326
Fnu4HI GCNGC 8 cut(s) 552, 784, 819, 822, 1182, 2118, 2121, 2442
FokI GGATG 4 cut(s) 92, 793, 1667, 1850
FriOI GRGCYC 2 cut(s) 201, 367
Fsp4HI GCNGC 8 cut(s) 552, 784, 819, 822, 1182, 2118, 2121, 2442
FspBI CTAG 8 cut(s) 1089, 1349, 1391, 1505, 1970, 2081, 2150, 2373
GlaI GCGC 3 cut(s) 671, 1628, 2398
GluI GCNGC 8 cut(s) 552, 784, 819, 822, 1182, 2118, 2121, 2442
GsaI CCCAGC 2 cut(s) 166, 1204
GsuI CTGGAG 4 cut(s) 91, 194, 972, 1803
HaeIII GGCC 5 cut(s) 343, 1158, 1536, 1934, 2212
HapII CCGG 3 cut(s) 138, 368, 1590
HhaI GCGC 3 cut(s) 672, 1629, 2399
Hin6I GCGC 3 cut(s) 670, 1627, 2397
HinP1I GCGC 3 cut(s) 670, 1627, 2397
HincII GTYRAC 2 cut(s) 318, 775
HindII GTYRAC 2 cut(s) 318, 775
HindIII AAGCTT 3 cut(s) 397, 591, 1791
HpaI GTTAAC 1 cut(s) 318
HpaII CCGG 3 cut(s) 138, 368, 1590
HphI GGTGA 4 cut(s) 492, 1240, 1441, 2366
Hpy166II GTNNAC 4 cut(s) 318, 775, 2061, 2092
Hpy8I GTNNAC 4 cut(s) 318, 775, 2061, 2092
HpyAV CCTTC 6 cut(s) 566, 1051, 1286, 1864, 1901, 2044
HpyCH4III ACNGT 3 cut(s) 37, 390, 1577
HpyCH4IV ACGT 5 cut(s) 379, 691, 1080, 1233, 1675
HpySE526I ACGT 5 cut(s) 379, 691, 1080, 1233, 1675
HspAI GCGC 3 cut(s) 670, 1627, 2397
Kpn2I TCCGGA 1 cut(s) 367
Ksp22I TGATCA 2 cut(s) 790, 2326
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
LmnI GCTCC 5 cut(s) 116, 146, 370, 1150, 1690
Lsp1109I GCAGC 7 cut(s) 563, 770, 833, 1193, 2104, 2132, 2428
LweI GCATC 6 cut(s) 70, 214, 1443, 1559, 1828, 2095
MaeI CTAG 8 cut(s) 1089, 1349, 1391, 1505, 1970, 2081, 2150, 2373
MaeII ACGT 5 cut(s) 379, 691, 1080, 1233, 1675
MaeIII GTNAC 4 cut(s) 37, 1414, 1477, 2390
MalI GATC 9 cut(s) 480, 724, 792, 957, 2176, 2328, 2350, 2456, 2481
MboI GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
MflI RGATCY 3 cut(s) 955, 2348, 2479
MhlI GDGCHC 4 cut(s) 201, 367, 1302, 2322
MlsI TGGCCA 1 cut(s) 1158
MluNI TGGCCA 1 cut(s) 1158
MlyI GAGTC 4 cut(s) 122, 128, 422, 1437
MmeI TCCRAC 8 cut(s) 150, 156, 240, 712, 1096, 1632, 2002, 2432
Mox20I TGGCCA 1 cut(s) 1158
MroI TCCGGA 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 1998
MscI TGGCCA 1 cut(s) 1158
MseI TTAA 8 cut(s) 243, 317, 458, 714, 1265, 1365, 1712, 2403
MslI CAYNNNNRTG 1 cut(s) 1542
Msp20I TGGCCA 1 cut(s) 1158
MspA1I CMGCKG 2 cut(s) 554, 1184
MspCI CTTAAG 1 cut(s) 1264
MspI CCGG 3 cut(s) 138, 368, 1590
MspR9I CCNGG 5 cut(s) 173, 1111, 1501, 1590, 2316
Mva1269I GAATGC 1 cut(s) 672
MvaI CCWGG 4 cut(s) 173, 1111, 1501, 2316
MvnI CGCG 1 cut(s) 2399
NciI CCSGG 1 cut(s) 1590
NdeI CATATG 1 cut(s) 2086
NdeII GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
NlaIV GGNNCC 7 cut(s) 142, 366, 372, 766, 1933, 2427, 2481
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 37, 2390
NspI RCATGY 3 cut(s) 542, 566, 2527
NspV TTCGAA 1 cut(s) 2041
PaeI GCATGC 1 cut(s) 542
PagI TCATGA 1 cut(s) 921
PaqCI CACCTGC 1 cut(s) 511
PasI CCCWGGG 1 cut(s) 1110
PciI ACATGT 1 cut(s) 2523
PctI GAATGC 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 1998
PfeI GAWTC 9 cut(s) 116, 122, 178, 247, 494, 1352, 1529, 1727, 2369
PflMI CCANNNNNTGG 1 cut(s) 2189
PkrI GCNGC 8 cut(s) 553, 785, 820, 823, 1183, 2119, 2122, 2443
PleI GAGTC 4 cut(s) 122, 128, 421, 1436
PpsI GAGTC 4 cut(s) 122, 128, 421, 1436
Ppu21I YACGTR 1 cut(s) 1234
PscI ACATGT 1 cut(s) 2523
PsiI TTATAA 3 cut(s) 849, 1073, 2226
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 4 cut(s) 171, 1109, 1499, 2314
PspFI CCCAGC 2 cut(s) 162, 1200
PspGI CCWGG 4 cut(s) 171, 1109, 1499, 2314
PspN4I GGNNCC 7 cut(s) 142, 366, 372, 766, 1933, 2427, 2481
PspPI GGNCC 5 cut(s) 341, 370, 1534, 1716, 1932
PstI CTGCAG 3 cut(s) 553, 788, 2122
PsuI RGATCY 3 cut(s) 955, 2348, 2479
PvuII CAGCTG 2 cut(s) 554, 1184
RsaI GTAC 3 cut(s) 1232, 1486, 1944
RsaNI GTAC 3 cut(s) 1231, 1485, 1943
RseI CAYNNNNRTG 1 cut(s) 1542
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 8 cut(s) 243, 317, 458, 714, 1265, 1365, 1712, 2403
SatI GCNGC 8 cut(s) 552, 784, 819, 822, 1182, 2118, 2121, 2442
Sau3AI GATC 9 cut(s) 478, 722, 790, 955, 2174, 2326, 2348, 2454, 2479
Sau96I GGNCC 5 cut(s) 341, 370, 1534, 1716, 1932
SchI GAGTC 4 cut(s) 122, 128, 422, 1437
ScrFI CCNGG 5 cut(s) 173, 1111, 1501, 1590, 2316
SduI GDGCHC 4 cut(s) 201, 367, 1302, 2322
SfaNI GCATC 6 cut(s) 70, 214, 1443, 1559, 1828, 2095
SfcI CTRYAG 6 cut(s) 386, 549, 784, 1005, 1573, 2118
SfuI TTCGAA 1 cut(s) 2041
SinI GGWCC 2 cut(s) 370, 1716
SmiMI CAYNNNNRTG 1 cut(s) 1542
SmlI CTYRAG 1 cut(s) 1264
SmoI CTYRAG 1 cut(s) 1264
SphI GCATGC 1 cut(s) 542
SsiI CCGC 3 cut(s) 819, 1252, 2423
SspMI CTAG 8 cut(s) 1089, 1349, 1391, 1505, 1970, 2081, 2150, 2373
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 5 cut(s) 171, 1109, 1499, 1588, 2314
StyI CCWWGG 6 cut(s) 344, 516, 1146, 1305, 1926, 2383
TaaI ACNGT 3 cut(s) 37, 390, 1577
TaiI ACGT 5 cut(s) 382, 694, 1083, 1236, 1678
TaqI TCGA 4 cut(s) 189, 469, 1662, 2041
TatI WGTACW 1 cut(s) 1942
TauI GCSGC 1 cut(s) 821
TfiI GAWTC 9 cut(s) 116, 122, 178, 247, 494, 1352, 1529, 1727, 2369
Tru1I TTAA 8 cut(s) 243, 317, 458, 714, 1265, 1365, 1712, 2403
Tru9I TTAA 8 cut(s) 243, 317, 458, 714, 1265, 1365, 1712, 2403
TscAI CASTG 8 cut(s) 91, 784, 793, 1600, 2020, 2068, 2329, 2350
TseFI GTSAC 2 cut(s) 37, 2390
TseI GCWGC 7 cut(s) 551, 783, 821, 1181, 2117, 2120, 2441
Tsp45I GTSAC 2 cut(s) 37, 2390
TspGWI ACGGA 2 cut(s) 471, 475
TspRI CASTG 8 cut(s) 91, 784, 793, 1600, 2020, 2068, 2329, 2350
Van91I CCANNNNNTGG 1 cut(s) 2189
Vha464I CTTAAG 1 cut(s) 1264
VpaK11BI GGWCC 2 cut(s) 370, 1716
XapI RAATTY 7 cut(s) 460, 663, 843, 935, 1583, 1753, 2068
XbaI TCTAGA 3 cut(s) 1390, 1969, 2372
XceI RCATGY 3 cut(s) 542, 566, 2527
XmnI GAANNNNTTC 1 cut(s) 1998
XspI CTAG 8 cut(s) 1089, 1349, 1391, 1505, 1970, 2081, 2150, 2373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.