RchiOBHm_Chr7g0219031

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004707
Physical Location & Seq
Forward (+)
37212062 .. 37220744
8683 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19605

Sequence Viewer

Length: 2547 bp
ATGCAACAAAACCCACACTCCAGTGCAGCGAGAGAGAGAGAGAAGAAAAACCCAATAGAAATCAAATCAGAGATGGAGGAAGAAACCCTAAATCCTGAACCAGAGACCTTGACTTTGCAAGAGAACAACAATGGAGACCAACCCATGCTGGATGCCGCTGACCAAAATCCTAACTCCTCCAGCTCCGATTCTGATTCCGACTCCGGCTCCGGCTCCGATGACGAAGACCAAGCCCAGCAAAACCTGGAGATTCAAACACTCGAAGCCGAGCTCGCAACCAACCCCTCCAACTACGATGCCCATGTCCAATACATTCAGATTCTGAGGAAAATGGCTGACATTGAAAAGCTGAGGCGAGCGAGGGAAGCTATGAGTATGCTGTTGCCTTTAACCCCCTCTATGTGGCAGGATTGGGCTAAGGACGAACTTGCTATGGGCTCCGGACCCGAGACGTTTTCTACAGTTGAAAAGCTTTATGAGCAAGGAGTCTCTGATTATCTGTCCATCTCTCTTTGGCGAGACTACTTAAATTTTGTCCGAGAAAATGATCCGTCAGTAAAAGAATCTTCACCTGCTGGTATTTCGAAGGAAAGAGATTTATTTGAGCGTGCTCTTACTGCAGCTGGTTTGCATGTATCTGAAGGCAATAAGATATGGGAAGCTTACAGGGAGTTTGAGCAAGCTATATGTGATACTATCGATGGGACTGACACTCAGGCAAGAGAGAAGCAAATTCAGCGCATTCGTGTTATATTCCAACGTCAGCTATCAGTTCCCCATGTTAATATGAGATCAACACTTTTGGCTTACAAGGCATGGGAGATGGAACAAGGAACCATTGTTGACACTGGCTTCAGTGATCAGGATGGTATTTCATCTCATGTTGCCGCAGCATACCAAAAGGCATTGGAAATTTATAATGCCCGTGTTCATTTTGAAGAACAAATCAGTCAGCATGATATGCCTGAGATAGAAAGACTGGAAAAGTTCATGAACTACTTGAAATTCGAGCAATCTTCTGGAGATCCTGCCCGTGTTCAAGTGTTGTATGAACGGGCTATTGCTGAGTTTCCTATAGCCAGTCAGCTCTGGCTTGATTATACTTCCTATTTGGACAAAACTTTGAAGGTTGGCAGAATTATAAGCAACGTTTATTCTAGGGCTGTAAAGAATTGCCCCTGGGTTGGAGAACTTTGGGTCAGATATTTGCTTTCCTTGGAGCGTGGCCATGCTTCTGAGAAAGAGATTGCAGCTGTGTTTTACAAGTCCCAGCAATGCACCTTTTCAACCCTTGATGAGTACGTGGAGTTGTTTCTCACCCGCATAGATGGCTTAAGACGAAGACTATCATGCCCCGTTGAAGGAGAGCACGCCTTGGATTATTCATTGTTCAGGGATACATTTCAGTCTGCATCGGATTACCTATCACCACAGATGAAGAACACAGATGTCCTGTTACGTTTGTATGCTTATTGGGCCCGCCTAGAGCTACATCTGGGAAAAGATTTAGTTGCTGCTCGGGGGGTTTGGGAAAGTTTACTGAAGATCAGTGGTACGATGATGGAAGCCTGGCTAGGTTATATAGCAATGGAAATTGAATCGGGCCATGTGAATGAAGCTCGGTCCATATATAGGAGATGCTACGGTAAAAAATTTTCCGGCACTGGTTCAGAGGACATTTGCTACTTGTGGTTGCGCTTTGAGAGGGAGTTTGGGTCGTTGGATGATTTCGACTATGCAGAACGTAAGGTTGCTCCCCGTCTGCAAGAGCTTCAACTATTAAGGTCCAAGCAAGAATCCAAATTGACTGAAGAGAGGGAGAATTCTCTAAAAAAGAATGTGCGTGAGAAGAGAAAGCAAGCTTCAGATGTAAGTGATGAACAACCTCCAGCAAAGAGGCAAAAGGATGCAACTCAAAAGCAAAAGAAAGTGCATGGGAAGGATTATGAGGTGCAGAACAAAGCTGAACAAAATGAAGGGAAAGAGATGAAAGCCAAGGGGGCCAAAGATGTACAAAATGAGATGAAAGAGCCTGTTCTAGAGAAAACAAAAGTATACGCTGACCAATGCACTGCTTTTGTATCAAACCTTAGCTTAAAGGCAAATAATGAACATCTTCATCAGTTCTTCAGTGATGTTGGAGGAGTTGTTGCTATTCGAATACTGAAGGACAAGTTCACTGGAAATTCAAGGGGACTAGCATATGTGGACTTCTCAGATGATGCACACCTTGCTGCAGCCATAGCAAAAAATAAACAGACACTCCTAGACAGGAAGCTGAGCATTGCACGATCAAATCCAAAGCGTGGCAAGAAAGAACATGGGCACACTGATCAAACTGGGGCAGCCAGTGGATCTGGTGAGACCTCCAATGAACCTAGAGCACGCCAAGGAAGCGACAGTGCGATTAAGAGGAAGAACACATTTGCGGTTCCGAGGAATGTTGCTGCACTTGGTTGGATCACGAATAAGCCAAAAACTCAGGATCCAGATGATGAAAAGCCAAAATCTAATGATGAGTTCAGAAACATGTTCCTGAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

848

Amino Acids

96.65

Weight (kDa)

5.63

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 84 - 420 1.2e-29 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 87 - 269 1.2e-16 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 88 - 229 6.9e-06 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 308 - 390 7.5e-12 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 310 - 406 2.5e-07 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 331 - 438 9.6e-07 PRP39 N-terminal HAT repeat
RRM_1 PF00076 693 - 760 4.2e-14 RNA recognition motif
Lsm_interact PF05391 830 - 847 1.7e-07 Lsm interaction motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 918, 1142
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 2306
AccI GTMKAC 1 cut(s) 2055
AccIII TCCGGA 1 cut(s) 440
AciI CCGC 5 cut(s) 156, 888, 1321, 1480, 2429
AclI AACGTT 1 cut(s) 1149
AclWI GGATC 6 cut(s) 542, 1019, 2362, 2468, 2480, 2493
AcoI YGGCCR 1 cut(s) 1225
AcsI RAATTY 7 cut(s) 529, 732, 912, 1004, 1652, 1822, 2185
AcuI CTGAAG 7 cut(s) 660, 838, 1562, 1830, 1848, 2113, 2186
AfaI GTAC 3 cut(s) 1301, 1555, 2013
AfiI CCNNNNNNNGG 5 cut(s) 402, 1184, 1361, 1632, 2306
AflII CTTAAG 1 cut(s) 1333
AflIII ACRYGT 1 cut(s) 2529
AjnI CCWGG 3 cut(s) 243, 1178, 1568
AleI CACNNNNGTG 1 cut(s) 21
Alw21I GWGCWC 4 cut(s) 273, 613, 1371, 2386
Alw26I GTCTC 6 cut(s) 98, 129, 443, 493, 513, 2357
AlwI GGATC 6 cut(s) 542, 1019, 2362, 2468, 2480, 2493
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 2 cut(s) 446, 1518
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 4 cut(s) 1225, 1476, 1603, 2001
ApaI GGGCCC 1 cut(s) 1480
ApeKI GCWGC 9 cut(s) 26, 620, 890, 1250, 1514, 2234, 2237, 2345, 2447
ApoI RAATTY 7 cut(s) 529, 732, 912, 1004, 1652, 1822, 2185
ArsI GACNNNNNNTTYG 2 cut(s) 97, 129
Asp700I GAANNNNTTC 1 cut(s) 2115
AspLEI GCGC 2 cut(s) 741, 1698
AspS9I GGNCC 7 cut(s) 443, 1476, 1477, 1603, 1623, 1785, 2001
AsuHPI GGTGA 4 cut(s) 561, 1309, 1419, 2372
AsuII TTCGAA 2 cut(s) 584, 2158
AvaI CYCGRG 2 cut(s) 446, 1518
AvaII GGWCC 3 cut(s) 443, 1623, 1785
BaeGI GKGCMC 2 cut(s) 1480, 2328
BalI TGGCCA 1 cut(s) 1227
BamHI GGATCC 1 cut(s) 2485
BanII GRGCYC 3 cut(s) 273, 440, 1480
BbsI GAAGAC 2 cut(s) 231, 1348
Bbv12I GWGCWC 4 cut(s) 273, 613, 1371, 2386
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 9 cut(s) 38, 632, 902, 1262, 1501, 2221, 2249, 2357, 2434
BccI CCATC 7 cut(s) 67, 512, 695, 817, 860, 1322, 1555
BciT130I CCWGG 3 cut(s) 245, 1180, 1570
BciVI GTATCC 1 cut(s) 1390
BclI TGATCA 2 cut(s) 859, 2332
BcoDI GTCTC 6 cut(s) 98, 129, 443, 493, 513, 2357
BfaI CTAG 7 cut(s) 1158, 1484, 1574, 2039, 2198, 2267, 2379
BfmI CTRYAG 4 cut(s) 459, 618, 1074, 2235
BfrI CTTAAG 1 cut(s) 1333
BfuAI ACCTGC 1 cut(s) 580
BfuI GTATCC 1 cut(s) 1390
BglI GCCNNNNNGGC 1 cut(s) 2000
BlpI GCTNAGC 1 cut(s) 2279
Bme1390I CCNGG 3 cut(s) 245, 1180, 1570
Bme18I GGWCC 3 cut(s) 443, 1623, 1785
BmeT110I CYCGRG 2 cut(s) 446, 1518
BmgT120I GGNCC 7 cut(s) 443, 1476, 1477, 1603, 1623, 1785, 2001
BmiI GGNNCC 9 cut(s) 208, 214, 439, 445, 835, 1478, 2002, 2433, 2487
BmrFI CCNGG 3 cut(s) 245, 1180, 1570
BmrI ACTGGG 1 cut(s) 2349
BmsI GCATC 6 cut(s) 142, 286, 1421, 1628, 1897, 2212
BmuI ACTGGG 1 cut(s) 2349
BpiI GAAGAC 2 cut(s) 231, 1348
BpmI CTGGAG 5 cut(s) 4, 163, 266, 1041, 1872
Bpu10I CCTNAGC 3 cut(s) 350, 417, 2090
Bpu1102I GCTNAGC 1 cut(s) 2279
Bpu14I TTCGAA 2 cut(s) 584, 2158
Bsa29I ATCGAT 1 cut(s) 699
BsaAI YACGTR 1 cut(s) 1303
BsaI GGTCTC 3 cut(s) 98, 129, 2357
BsaJI CCNNGG 7 cut(s) 1178, 1179, 1215, 1374, 1995, 2389, 2435
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 9 cut(s) 32, 191, 221, 1700, 1730, 2133, 2163, 2247, 2277
Bsc4I CCNNNNNNNGG 5 cut(s) 402, 1184, 1361, 1632, 2306
Bse1I ACTGG 8 cut(s) 21, 853, 984, 1080, 1669, 2185, 2344, 2349
Bse3DI GCAATG 3 cut(s) 1280, 1593, 2283
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 3 cut(s) 245, 1180, 1570
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 7 cut(s) 1178, 1179, 1215, 1374, 1995, 2389, 2435
BseGI GGATG 4 cut(s) 157, 871, 1729, 1912
BseLI CCNNNNNNNGG 5 cut(s) 402, 1184, 1361, 1632, 2306
BseMI GCAATG 3 cut(s) 1280, 1593, 2283
BseMII CTCAG 9 cut(s) 314, 341, 728, 957, 1056, 1227, 2229, 2270, 2495
BseNI ACTGG 8 cut(s) 21, 853, 984, 1080, 1669, 2185, 2344, 2349
BseRI GAGGAG 2 cut(s) 166, 2157
BseSI GKGCMC 2 cut(s) 1480, 2328
BseXI GCAGC 9 cut(s) 38, 632, 902, 1262, 1501, 2221, 2249, 2357, 2434
BseYI CCCAGC 2 cut(s) 234, 1269
BsgI GTGCAG 3 cut(s) 45, 1973, 2433
BshFI GGCC 4 cut(s) 1227, 1478, 1605, 2003
BshVI ATCGAT 1 cut(s) 699
BsiHKAI GWGCWC 4 cut(s) 273, 613, 1371, 2386
BsiHKCI CYCGRG 2 cut(s) 446, 1518
BsiSI CCGG 4 cut(s) 204, 210, 441, 1659
BslFI GGGAC 3 cut(s) 718, 1252, 2208
BslI CCNNNNNNNGG 5 cut(s) 402, 1184, 1361, 1632, 2306
BsmAI GTCTC 6 cut(s) 98, 129, 443, 493, 513, 2357
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 3 cut(s) 718, 1252, 2208
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 4 cut(s) 1227, 1478, 1605, 2003
Bso31I GGTCTC 3 cut(s) 98, 129, 2357
BsoBI CYCGRG 2 cut(s) 446, 1518
Bsp119I TTCGAA 2 cut(s) 584, 2158
Bsp120I GGGCCC 1 cut(s) 1476
Bsp1286I GDGCHC 7 cut(s) 273, 440, 613, 1371, 1480, 2328, 2386
Bsp13I TCCGGA 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 2011
Bsp1720I GCTNAGC 1 cut(s) 2279
BspACI CCGC 5 cut(s) 156, 888, 1321, 1480, 2429
BspANI GGCC 4 cut(s) 1227, 1478, 1605, 2003
BspCNI CTCAG 9 cut(s) 315, 342, 727, 958, 1057, 1228, 2228, 2271, 2494
BspDI ATCGAT 1 cut(s) 699
BspEI TCCGGA 1 cut(s) 440
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 9 cut(s) 208, 214, 439, 445, 835, 1478, 2002, 2433, 2487
BspMAI CTGCAG 2 cut(s) 622, 2239
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 6 cut(s) 542, 1019, 2362, 2468, 2480, 2493
BspT104I TTCGAA 2 cut(s) 584, 2158
BspTI CTTAAG 1 cut(s) 1333
BspTNI GGTCTC 3 cut(s) 98, 129, 2357
BsrDI GCAATG 3 cut(s) 1280, 1593, 2283
BsrGI TGTACA 1 cut(s) 2011
BsrI ACTGG 8 cut(s) 21, 853, 984, 1080, 1669, 2185, 2344, 2349
BssECI CCNNGG 7 cut(s) 1178, 1179, 1215, 1374, 1995, 2389, 2435
BssNAI GTATAC 1 cut(s) 2056
BssT1I CCWWGG 4 cut(s) 1215, 1374, 1995, 2389
Bst1107I GTATAC 1 cut(s) 2056
Bst2UI CCWGG 3 cut(s) 245, 1180, 1570
Bst4CI ACNGT 3 cut(s) 463, 1646, 2402
Bst6I CTCTTC 2 cut(s) 1806, 1844
BstAFI CTTAAG 1 cut(s) 1333
BstAPI GCANNNNNTGC 2 cut(s) 961, 2231
BstAUI TGTACA 1 cut(s) 2011
BstBAI YACGTR 1 cut(s) 1303
BstBI TTCGAA 2 cut(s) 584, 2158
BstC8I GCNNGC 8 cut(s) 273, 357, 609, 681, 1371, 1480, 1860, 2386
BstF5I GGATG 4 cut(s) 157, 871, 1729, 1912
BstHHI GCGC 2 cut(s) 741, 1698
BstMAI GTCTC 6 cut(s) 98, 129, 443, 493, 513, 2357
BstNI CCWGG 3 cut(s) 245, 1180, 1570
BstNSI RCATGY 2 cut(s) 635, 2533
BstSCI CCNGG 3 cut(s) 243, 1178, 1568
BstSFI CTRYAG 4 cut(s) 459, 618, 1074, 2235
BstSLI GKGCMC 2 cut(s) 1480, 2328
BstV1I GCAGC 9 cut(s) 38, 632, 902, 1262, 1501, 2221, 2249, 2357, 2434
BstV2I GAAGAC 2 cut(s) 231, 1348
BstX2I RGATCY 3 cut(s) 1024, 2354, 2485
BstYI RGATCY 3 cut(s) 1024, 2354, 2485
BstZ17I GTATAC 1 cut(s) 2056
Bsu15I ATCGAT 1 cut(s) 699
BsuI GTATCC 1 cut(s) 1390
BsuRI GGCC 4 cut(s) 1227, 1478, 1605, 2003
BsuTUI ATCGAT 1 cut(s) 699
BtsCI GGATG 4 cut(s) 157, 871, 1729, 1912
BtsI GCAGTG 1 cut(s) 2070
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 8 cut(s) 273, 357, 609, 681, 1371, 1480, 1860, 2386
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 1 cut(s) 990
CfoI GCGC 2 cut(s) 741, 1698
Cfr13I GGNCC 7 cut(s) 443, 1476, 1477, 1603, 1623, 1785, 2001
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 3 cut(s) 1300, 1554, 2012
CviQI GTAC 3 cut(s) 1300, 1554, 2012
EaeI YGGCCR 1 cut(s) 1225
Eam1104I CTCTTC 2 cut(s) 1806, 1844
EarI CTCTTC 2 cut(s) 1806, 1844
Ecl136II GAGCTC 1 cut(s) 271
Eco130I CCWWGG 4 cut(s) 1215, 1374, 1995, 2389
Eco24I GRGCYC 3 cut(s) 273, 440, 1480
Eco31I GGTCTC 3 cut(s) 98, 129, 2357
Eco47I GGWCC 3 cut(s) 443, 1623, 1785
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 7 cut(s) 660, 838, 1562, 1830, 1848, 2113, 2186
Eco88I CYCGRG 2 cut(s) 446, 1518
EcoICRI GAGCTC 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 1822
EcoRII CCWGG 3 cut(s) 243, 1178, 1568
EcoT14I CCWWGG 4 cut(s) 1215, 1374, 1995, 2389
EcoT38I GRGCYC 3 cut(s) 273, 440, 1480
ErhI CCWWGG 4 cut(s) 1215, 1374, 1995, 2389
Esp3I CGTCTC 1 cut(s) 443
FaqI GGGAC 3 cut(s) 718, 1252, 2208
FauI CCCGC 2 cut(s) 1328, 1487
FauNDI CATATG 1 cut(s) 2203
FbaI TGATCA 2 cut(s) 859, 2332
FblI GTMKAC 1 cut(s) 2055
FokI GGATG 4 cut(s) 164, 878, 1736, 1919
FriOI GRGCYC 3 cut(s) 273, 440, 1480
FspBI CTAG 7 cut(s) 1158, 1484, 1574, 2039, 2198, 2267, 2379
GlaI GCGC 2 cut(s) 740, 1697
GsaI CCCAGC 2 cut(s) 238, 1273
GsuI CTGGAG 5 cut(s) 4, 163, 266, 1041, 1872
HaeIII GGCC 4 cut(s) 1227, 1478, 1605, 2003
HapII CCGG 4 cut(s) 204, 210, 441, 1659
HhaI GCGC 2 cut(s) 741, 1698
Hin6I GCGC 2 cut(s) 739, 1696
HinP1I GCGC 2 cut(s) 739, 1696
HincII GTYRAC 1 cut(s) 844
HindII GTYRAC 1 cut(s) 844
HindIII AAGCTT 3 cut(s) 470, 660, 1860
HinfI GANTC 9 cut(s) 188, 194, 200, 250, 319, 486, 563, 1598, 1796
HpaII CCGG 4 cut(s) 204, 210, 441, 1659
HphI GGTGA 4 cut(s) 561, 1309, 1419, 2372
Hpy166II GTNNAC 5 cut(s) 844, 1538, 2056, 2178, 2209
Hpy8I GTNNAC 5 cut(s) 844, 1538, 2056, 2178, 2209
HpyAV CCTTC 7 cut(s) 580, 635, 1120, 1355, 1933, 1970, 2161
HpyCH4III ACNGT 3 cut(s) 463, 1646, 2402
HpyCH4IV ACGT 6 cut(s) 452, 760, 1149, 1302, 1459, 1744
HpySE526I ACGT 6 cut(s) 452, 760, 1149, 1302, 1459, 1744
HspAI GCGC 2 cut(s) 739, 1696
Kpn2I TCCGGA 1 cut(s) 440
Ksp22I TGATCA 2 cut(s) 859, 2332
LmnI GCTCC 6 cut(s) 188, 212, 218, 443, 1219, 1759
Lsp1109I GCAGC 9 cut(s) 38, 632, 902, 1262, 1501, 2221, 2249, 2357, 2434
LweI GCATC 6 cut(s) 142, 286, 1421, 1628, 1897, 2212
MaeI CTAG 7 cut(s) 1158, 1484, 1574, 2039, 2198, 2267, 2379
MaeII ACGT 6 cut(s) 452, 760, 1149, 1302, 1459, 1744
MaeIII GTNAC 1 cut(s) 1455
MflI RGATCY 3 cut(s) 1024, 2354, 2485
MhlI GDGCHC 7 cut(s) 273, 440, 613, 1371, 1480, 2328, 2386
MlsI TGGCCA 1 cut(s) 1227
MluNI TGGCCA 1 cut(s) 1227
MlyI GAGTC 2 cut(s) 194, 495
MmeI TCCRAC 7 cut(s) 222, 312, 781, 1165, 1701, 2119, 2438
Mox20I TGGCCA 1 cut(s) 1227
MroI TCCGGA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 2115
MscI TGGCCA 1 cut(s) 1227
MseI TTAA 7 cut(s) 389, 527, 783, 1334, 1781, 2096, 2409
MslI CAYNNNNRTG 2 cut(s) 21, 1611
Msp20I TGGCCA 1 cut(s) 1227
MspA1I CMGCKG 3 cut(s) 158, 623, 1253
MspCI CTTAAG 1 cut(s) 1333
MspI CCGG 4 cut(s) 204, 210, 441, 1659
MspR9I CCNGG 3 cut(s) 245, 1180, 1570
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 3 cut(s) 245, 1180, 1570
NdeI CATATG 1 cut(s) 2203
NlaIV GGNNCC 9 cut(s) 208, 214, 439, 445, 835, 1478, 2002, 2433, 2487
NmeAIII GCCGAG 1 cut(s) 292
NspI RCATGY 2 cut(s) 635, 2533
NspV TTCGAA 2 cut(s) 584, 2158
OliI CACNNNNGTG 1 cut(s) 21
PagI TCATGA 1 cut(s) 990
PaqCI CACCTGC 1 cut(s) 580
PasI CCCWGGG 1 cut(s) 1179
PciI ACATGT 1 cut(s) 2529
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 2115
PfeI GAWTC 7 cut(s) 188, 194, 250, 319, 563, 1598, 1796
PflMI CCANNNNNTGG 1 cut(s) 2306
PleI GAGTC 2 cut(s) 194, 494
PpsI GAGTC 2 cut(s) 194, 494
Ppu21I YACGTR 1 cut(s) 1303
PscI ACATGT 1 cut(s) 2529
PsiI TTATAA 2 cut(s) 918, 1142
Psp124BI GAGCTC 1 cut(s) 273
Psp1406I AACGTT 1 cut(s) 1149
Psp6I CCWGG 3 cut(s) 243, 1178, 1568
PspFI CCCAGC 2 cut(s) 234, 1269
PspGI CCWGG 3 cut(s) 243, 1178, 1568
PspN4I GGNNCC 9 cut(s) 208, 214, 439, 445, 835, 1478, 2002, 2433, 2487
PspOMI GGGCCC 1 cut(s) 1476
PspPI GGNCC 7 cut(s) 443, 1476, 1477, 1603, 1623, 1785, 2001
PstI CTGCAG 2 cut(s) 622, 2239
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 3 cut(s) 1024, 2354, 2485
PvuII CAGCTG 2 cut(s) 623, 1253
RsaI GTAC 3 cut(s) 1301, 1555, 2013
RsaNI GTAC 3 cut(s) 1300, 1554, 2012
RseI CAYNNNNRTG 2 cut(s) 21, 1611
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 7 cut(s) 389, 527, 783, 1334, 1781, 2096, 2409
Sau96I GGNCC 7 cut(s) 443, 1476, 1477, 1603, 1623, 1785, 2001
SchI GAGTC 2 cut(s) 194, 495
ScrFI CCNGG 3 cut(s) 245, 1180, 1570
SduI GDGCHC 7 cut(s) 273, 440, 613, 1371, 1480, 2328, 2386
SfaNI GCATC 6 cut(s) 142, 286, 1421, 1628, 1897, 2212
SfcI CTRYAG 4 cut(s) 459, 618, 1074, 2235
SfuI TTCGAA 2 cut(s) 584, 2158
SinI GGWCC 3 cut(s) 443, 1623, 1785
SmiMI CAYNNNNRTG 2 cut(s) 21, 1611
SmlI CTYRAG 1 cut(s) 1333
SmoI CTYRAG 1 cut(s) 1333
SsiI CCGC 5 cut(s) 156, 888, 1321, 1480, 2429
SspMI CTAG 7 cut(s) 1158, 1484, 1574, 2039, 2198, 2267, 2379
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 3 cut(s) 243, 1178, 1568
StyI CCWWGG 4 cut(s) 1215, 1374, 1995, 2389
TaaI ACNGT 3 cut(s) 463, 1646, 2402
TaiI ACGT 6 cut(s) 455, 763, 1152, 1305, 1462, 1747
TaqI TCGA 6 cut(s) 261, 584, 699, 1008, 1731, 2158
TaqII GACCGA 1 cut(s) 1611
TatI WGTACW 1 cut(s) 2011
TauI GCSGC 2 cut(s) 158, 890
TfiI GAWTC 7 cut(s) 188, 194, 250, 319, 563, 1598, 1796
Tru1I TTAA 7 cut(s) 389, 527, 783, 1334, 1781, 2096, 2409
Tru9I TTAA 7 cut(s) 389, 527, 783, 1334, 1781, 2096, 2409
TseI GCWGC 9 cut(s) 26, 620, 890, 1250, 1514, 2234, 2237, 2345, 2447
TspGWI ACGGA 1 cut(s) 540
Van91I CCANNNNNTGG 1 cut(s) 2306
Vha464I CTTAAG 1 cut(s) 1333
VpaK11BI GGWCC 3 cut(s) 443, 1623, 1785
XapI RAATTY 7 cut(s) 529, 732, 912, 1004, 1652, 1822, 2185
XbaI TCTAGA 1 cut(s) 2038
XceI RCATGY 2 cut(s) 635, 2533
XmiI GTMKAC 1 cut(s) 2055
XmnI GAANNNNTTC 1 cut(s) 2115
XspI CTAG 7 cut(s) 1158, 1484, 1574, 2039, 2198, 2267, 2379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.