Rh7BG315500

Squamous cell carcinoma antigen recognized by T-cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004707
Physical Location & Seq
Forward (+)
30461677 .. 30469765
8089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG315500.1

Sequence Viewer

Length: 2466 bp
ATGGAGGAAGAAACCCTAAATCCTGAACCAGAGACCGCGACTTCGCAAGAGAACAACAATGGAGACCAACCCATGCTGGATGCCGCTGTCCAAAATCCTAACTCCTCCAGCTCCGATTCCGATTCCGACTCCGGCTCCGACTCCGATGACGAAGACCAAGCCCAGCAAAACCTGGAGATTCAAACACTCGAAGCCGAGCTCGCAACCAACCCCTCCAACTACGATGCCCATGTCCAATACATTAAGATTCTGAGGAAAATGGCTGACATTGAAAAGCTGAGGCGAGCGAGGGAAGCTATGAGTAAGCTGTTGCCGTTAACCCCCTCCATGTGGCAGGATTGGGCCAAGGACGAACTTGCTATGGGCTCCGGACCCGAGACGTTTTCTACAGTCGAAAAGCTTTATGAGCAAGGAGTCTCTGATTATCTGTCCATCTCTCTTTGGCGAGACTACTTAAATTTTGTTCGAGAAAATGATCCGTCAGTAAAAGAATCTTCACCTGCTGGTATTTCCAAGGCAAGAGATTTATTTGAGCGTGCTCTTACTGCAGCTGGTTTGCATGTATCTGAAGGCAATAAGATATGGGAAGCTTACAGGGAGTTTGAGCAAGCTATATGTGATACTATTGATGGGACTGACACTCAGGCAAGAGAGAAGCAAATTCAGCGCATTCGTGTTATATTCCAACGTCAGCTATCAGTTTCCCATGTTAATATGAGATCAACACTATTGGCTCACAAGGCATGGGAGATGGAACAAGGAACCATTGTTGACGCTGGCTGCAGTGATCAGGATGGTATTGCATCTCATGTTGCCTCAGCATACCAAAAGGCATTGGAAATTTATAATGCCCGTGTTCATTTTGAAGAACAAATCAGTCAGCATGATATGCCTGAGATAGAAAGACTTGAAAAGTTCATGAACTACTTGAAATTTGAGCAATCTTCTGGAGATCCTGCCCGTGTTCAAGTGTTGTATGAACGGGCTATTGCTGAGTTCCCTATAGCCAGTCAGCTCTGGCTTGATTATACTTCCTATTTGGACAAAACTTTGAAGGTTGGCAGAATTATAAGCAACGTTTATTCTAGGGCTGTAAAGAATTGCCCCTGGGTTGGAGAACTTTGGGTCAGATATTTGCTTTCCTTGGAGCGTGGCCATGCTTCTGAGAAAGAGATTGCAGCTGTGTTTTACAAGTCCCAGCAATGCACCTTTTCAACCCTTGATGAGTACGTGGAGTTGTTTCTCACCCGCATAGATGGCTTAAGACGAAGACTCTCATGCCCCGTTGAAGGAGAGCACGCCTTGGATTATTCGTTGTTGAGGGATACATTTCAGTCTGCATCGGATTACCTATCACCACAGATGAAGAACACAGATGTCCTGTTACGTTTGTATGCTTATTGGGCCCGCCTAGAGCTACATCTGGGAAAAGATGTAGTTGCTGCTCGGGGGGTTTGGGAAAGTTTACTGAAGATCAGTGGTACGATGATGGAAGCCTGGCTAGGTTATATAGCAATGGAAATTGAATCGGGCCATGTGAATGAAGCTCGGTCCATATATAGGAGATGCTACAGTAAAAAATTTTCCGGCACTGGTTCAGAGGACATTTGCTACTTGTGGTTGCGCTTTGAGAGGGAGTTTGGGTCGTTGGATGATTTCGACTATGCAGAACGTAAGGTTGCTCACCGTCTGCAAGAGCTTCAACTATTAAGGTCCAAGCAAGAATCCAAATTGACTGAAGAGAGGGAGAATTCTCTCAAAAAGAATGTGCGTGAGAAGAGAAAGCAAGCTTCAGATGAACAACCTCCAGCAAAGAGGCAAAAGGATGCAACTCAAAAGCAAAAGAAAGTGCACGGGAAGGATTATGAGGTGCAGAACAAAGCTGAACAAAATGAAGGGAAAGAGATGAAAGCCAAGGGTGCCAAAGATGTACAAAATGAGATGAAAGAGCCTGTTCTAGAGAAAACAAAAGTATACGCTGACCAATGCACTGCTTTTGTATCAAACCTTAGCTTAGAGGCAAATAATGAACATCTTCATCAGTTCTTCAGTGATGTTGGAGGAGTTGTTGCTATTCGAATACTGAAGGACAAGTTCACTGGAAAGTCAAGGGGACTAGCATATGTGGACTTCTCAGATGATGCACACCTTGCTGCAGCCATAGCAAAAAATAAACAGACACTCCTGGACAAGAAGCTGAGCATTGCACGATCAAATCCAAAGCGTGGCAAGAAAGAACATGGGCACACTTATCAAACTGGAGCAGCCAGTGGATCTAGTGAGACCTCCAATGAACCTAGAGCACGCCAAGGAAGCGACAGTGCGATTAAGGGGAAGAACACATTTGCGGTTCCGAGGAATGTTACTGCACTTGGTTGGAGCACGAATAAGCCAAAAACTCAGGATCCAGATGATGAAAAGCCAAAATCTAATGATGAGTTCAGAAACATGTTCCTGAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lsm_interact RRM_1 Suf

Protein Analysis

821

Amino Acids

93.36

Weight (kDa)

5.74

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suf PF05843 60 - 396 7e-27 Suppressor of forked protein (Suf)
HAT_PRP39_N PF23240 63 - 234 2.7e-16 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 64 - 204 1.4e-06 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 284 - 366 7.4e-12 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 286 - 379 3.3e-07 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 307 - 414 7.6e-07 PRP39 N-terminal HAT repeat
RRM_1 PF00076 666 - 733 1.4e-14 RNA recognition motif
Lsm_interact PF05391 803 - 820 1.7e-07 Lsm interaction motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 846, 1070
AarI CACCTGC 1 cut(s) 508
Acc36I ACCTGC 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 1919
AccB7I CCANNNNNTGG 1 cut(s) 2225
AccI GTMKAC 1 cut(s) 1974
AccII CGCG 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 5 cut(s) 36, 84, 1249, 1408, 2348
AclI AACGTT 1 cut(s) 1077
AclWI GGATC 5 cut(s) 470, 947, 2281, 2399, 2412
AcoI YGGCCR 1 cut(s) 1153
AcsI RAATTY 6 cut(s) 457, 660, 840, 932, 1580, 1750
AcuI CTGAAG 6 cut(s) 588, 1490, 1758, 1776, 2032, 2105
AfaI GTAC 3 cut(s) 1229, 1483, 1932
AfiI CCNNNNNNNGG 5 cut(s) 330, 1112, 1289, 1560, 2225
AflII CTTAAG 1 cut(s) 1261
AflIII ACRYGT 1 cut(s) 2448
AjnI CCWGG 4 cut(s) 171, 1106, 1496, 2184
Alw21I GWGCWC 6 cut(s) 201, 541, 1299, 1854, 2305, 2384
Alw26I GTCTC 6 cut(s) 26, 57, 371, 421, 441, 2276
Alw44I GTGCAC 1 cut(s) 1850
AlwI GGATC 5 cut(s) 470, 947, 2281, 2399, 2412
Ama87I CYCGRG 2 cut(s) 374, 1446
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 4 cut(s) 342, 1153, 1404, 1531
ApaI GGGCCC 1 cut(s) 1408
ApaLI GTGCAC 1 cut(s) 1850
ApeKI GCWGC 7 cut(s) 548, 780, 1178, 1442, 2153, 2156, 2264
ApoI RAATTY 6 cut(s) 457, 660, 840, 932, 1580, 1750
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 2034
AspLEI GCGC 2 cut(s) 669, 1626
AspS9I GGNCC 7 cut(s) 342, 371, 1404, 1405, 1531, 1551, 1713
AsuHPI GGTGA 4 cut(s) 489, 1237, 1347, 1676
AsuII TTCGAA 1 cut(s) 2077
AvaI CYCGRG 2 cut(s) 374, 1446
AvaII GGWCC 3 cut(s) 371, 1551, 1713
BaeGI GKGCMC 3 cut(s) 1408, 1854, 2247
BalI TGGCCA 1 cut(s) 1155
BamHI GGATCC 1 cut(s) 2404
BanI GGYRCC 1 cut(s) 1919
BanII GRGCYC 3 cut(s) 201, 368, 1408
BbsI GAAGAC 2 cut(s) 159, 1276
Bbv12I GWGCWC 6 cut(s) 201, 541, 1299, 1854, 2305, 2384
BbvCI CCTCAGC 2 cut(s) 278, 817
BbvI GCAGC 7 cut(s) 560, 767, 1190, 1429, 2140, 2168, 2276
BccI CCATC 6 cut(s) 440, 623, 745, 788, 1250, 1483
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 4 cut(s) 173, 1108, 1498, 2186
BciVI GTATCC 1 cut(s) 1318
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 6 cut(s) 26, 57, 371, 421, 441, 2276
BfaI CTAG 7 cut(s) 1086, 1412, 1502, 1958, 2117, 2277, 2298
BfmI CTRYAG 6 cut(s) 387, 546, 781, 1002, 1570, 2154
BfrI CTTAAG 1 cut(s) 1261
BfuAI ACCTGC 1 cut(s) 508
BfuI GTATCC 1 cut(s) 1318
BisI GCNGC 8 cut(s) 84, 549, 781, 1179, 1443, 2154, 2157, 2265
BlpI GCTNAGC 1 cut(s) 2198
BlsI GCNGC 8 cut(s) 85, 550, 782, 1180, 1444, 2155, 2158, 2266
Bme1390I CCNGG 4 cut(s) 173, 1108, 1498, 2186
Bme18I GGWCC 3 cut(s) 371, 1551, 1713
BmeT110I CYCGRG 2 cut(s) 374, 1446
BmgT120I GGNCC 7 cut(s) 342, 371, 1404, 1405, 1531, 1551, 1713
BmiI GGNNCC 8 cut(s) 136, 367, 373, 763, 1406, 1921, 2352, 2406
BmrFI CCNGG 4 cut(s) 173, 1108, 1498, 2186
BmsI GCATC 7 cut(s) 70, 214, 812, 1349, 1556, 1816, 2131
BpiI GAAGAC 2 cut(s) 159, 1276
BpmI CTGGAG 5 cut(s) 91, 194, 969, 1791, 2280
Bpu10I CCTNAGC 3 cut(s) 278, 817, 2009
Bpu1102I GCTNAGC 1 cut(s) 2198
Bpu14I TTCGAA 1 cut(s) 2077
BsaAI YACGTR 1 cut(s) 1231
BsaI GGTCTC 3 cut(s) 26, 57, 2276
BsaJI CCNNGG 9 cut(s) 345, 513, 1106, 1107, 1143, 1302, 1914, 2308, 2354
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 1628, 1658, 2052, 2082, 2166, 2196
Bsc4I CCNNNNNNNGG 5 cut(s) 330, 1112, 1289, 1560, 2225
Bse1I ACTGG 5 cut(s) 1008, 1597, 2104, 2263, 2268
Bse3DI GCAATG 3 cut(s) 1208, 1521, 2202
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 4 cut(s) 173, 1108, 1498, 2186
BseDI CCNNGG 9 cut(s) 345, 513, 1106, 1107, 1143, 1302, 1914, 2308, 2354
BseGI GGATG 4 cut(s) 85, 799, 1657, 1831
BseLI CCNNNNNNNGG 5 cut(s) 330, 1112, 1289, 1560, 2225
BseMI GCAATG 3 cut(s) 1208, 1521, 2202
BseNI ACTGG 5 cut(s) 1008, 1597, 2104, 2263, 2268
BseRI GAGGAG 2 cut(s) 94, 2076
BseSI GKGCMC 3 cut(s) 1408, 1854, 2247
BseXI GCAGC 7 cut(s) 560, 767, 1190, 1429, 2140, 2168, 2276
BseYI CCCAGC 2 cut(s) 162, 1197
BsgI GTGCAG 2 cut(s) 1892, 2352
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 4 cut(s) 344, 1155, 1406, 1533
BshNI GGYRCC 1 cut(s) 1919
BsiHKAI GWGCWC 6 cut(s) 201, 541, 1299, 1854, 2305, 2384
BsiHKCI CYCGRG 2 cut(s) 374, 1446
BsiSI CCGG 3 cut(s) 132, 369, 1587
BslFI GGGAC 3 cut(s) 646, 1180, 2127
BslI CCNNNNNNNGG 5 cut(s) 330, 1112, 1289, 1560, 2225
BsmAI GTCTC 6 cut(s) 26, 57, 371, 421, 441, 2276
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 3 cut(s) 646, 1180, 2127
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 4 cut(s) 344, 1155, 1406, 1533
Bso31I GGTCTC 3 cut(s) 26, 57, 2276
BsoBI CYCGRG 2 cut(s) 374, 1446
Bsp119I TTCGAA 1 cut(s) 2077
Bsp120I GGGCCC 1 cut(s) 1404
Bsp1286I GDGCHC 9 cut(s) 201, 368, 541, 1299, 1408, 1854, 2247, 2305, 2384
Bsp13I TCCGGA 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 1930
Bsp143I GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
Bsp1720I GCTNAGC 1 cut(s) 2198
BspACI CCGC 5 cut(s) 36, 84, 1249, 1408, 2348
BspANI GGCC 4 cut(s) 344, 1155, 1406, 1533
BspEI TCCGGA 1 cut(s) 368
BspFNI CGCG 1 cut(s) 38
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 8 cut(s) 136, 367, 373, 763, 1406, 1921, 2352, 2406
BspMAI CTGCAG 3 cut(s) 550, 785, 2158
BspMI ACCTGC 1 cut(s) 508
BspPI GGATC 5 cut(s) 470, 947, 2281, 2399, 2412
BspT104I TTCGAA 1 cut(s) 2077
BspT107I GGYRCC 1 cut(s) 1919
BspTI CTTAAG 1 cut(s) 1261
BspTNI GGTCTC 3 cut(s) 26, 57, 2276
BsrDI GCAATG 3 cut(s) 1208, 1521, 2202
BsrGI TGTACA 1 cut(s) 1930
BsrI ACTGG 5 cut(s) 1008, 1597, 2104, 2263, 2268
BssECI CCNNGG 9 cut(s) 345, 513, 1106, 1107, 1143, 1302, 1914, 2308, 2354
BssMI GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
BssNAI GTATAC 1 cut(s) 1975
BssT1I CCWWGG 6 cut(s) 345, 513, 1143, 1302, 1914, 2308
Bst1107I GTATAC 1 cut(s) 1975
Bst2UI CCWGG 4 cut(s) 173, 1108, 1498, 2186
Bst4CI ACNGT 4 cut(s) 391, 1574, 1688, 2321
Bst6I CTCTTC 2 cut(s) 1734, 1772
BstAFI CTTAAG 1 cut(s) 1261
BstAPI GCANNNNNTGC 2 cut(s) 889, 2150
BstAUI TGTACA 1 cut(s) 1930
BstBAI YACGTR 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 2077
BstC8I GCNNGC 9 cut(s) 201, 285, 537, 609, 778, 1299, 1408, 1788, 2305
BstF5I GGATG 4 cut(s) 85, 799, 1657, 1831
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 2 cut(s) 669, 1626
BstKTI GATC 8 cut(s) 478, 722, 790, 955, 1476, 2213, 2276, 2407
BstMAI GTCTC 6 cut(s) 26, 57, 371, 421, 441, 2276
BstMBI GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
BstNI CCWGG 4 cut(s) 173, 1108, 1498, 2186
BstNSI RCATGY 2 cut(s) 563, 2452
BstSCI CCNGG 4 cut(s) 171, 1106, 1496, 2184
BstSFI CTRYAG 6 cut(s) 387, 546, 781, 1002, 1570, 2154
BstSLI GKGCMC 3 cut(s) 1408, 1854, 2247
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 7 cut(s) 560, 767, 1190, 1429, 2140, 2168, 2276
BstV2I GAAGAC 2 cut(s) 159, 1276
BstX2I RGATCY 3 cut(s) 952, 2273, 2404
BstYI RGATCY 3 cut(s) 952, 2273, 2404
BstZ17I GTATAC 1 cut(s) 1975
BsuI GTATCC 1 cut(s) 1318
BsuRI GGCC 4 cut(s) 344, 1155, 1406, 1533
BtsCI GGATG 4 cut(s) 85, 799, 1657, 1831
BtsI GCAGTG 2 cut(s) 790, 1989
BtsIMutI CAGTG 8 cut(s) 790, 1483, 1590, 1989, 2056, 2097, 2275, 2326
BveI ACCTGC 1 cut(s) 508
Cac8I GCNNGC 9 cut(s) 201, 285, 537, 609, 778, 1299, 1408, 1788, 2305
CciI TCATGA 1 cut(s) 918
CfoI GCGC 2 cut(s) 669, 1626
Cfr13I GGNCC 7 cut(s) 342, 371, 1404, 1405, 1531, 1551, 1713
CseI GACGC 1 cut(s) 782
Csp6I GTAC 3 cut(s) 1228, 1482, 1931
CviQI GTAC 3 cut(s) 1228, 1482, 1931
DpnI GATC 8 cut(s) 477, 721, 789, 954, 1475, 2212, 2275, 2406
DpnII GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
EaeI YGGCCR 1 cut(s) 1153
Eam1104I CTCTTC 2 cut(s) 1734, 1772
EarI CTCTTC 2 cut(s) 1734, 1772
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 6 cut(s) 345, 513, 1143, 1302, 1914, 2308
Eco24I GRGCYC 3 cut(s) 201, 368, 1408
Eco31I GGTCTC 3 cut(s) 26, 57, 2276
Eco47I GGWCC 3 cut(s) 371, 1551, 1713
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 6 cut(s) 588, 1490, 1758, 1776, 2032, 2105
Eco88I CYCGRG 2 cut(s) 374, 1446
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 1750
EcoRII CCWGG 4 cut(s) 171, 1106, 1496, 2184
EcoT14I CCWWGG 6 cut(s) 345, 513, 1143, 1302, 1914, 2308
EcoT38I GRGCYC 3 cut(s) 201, 368, 1408
ErhI CCWWGG 6 cut(s) 345, 513, 1143, 1302, 1914, 2308
Esp3I CGTCTC 1 cut(s) 371
FaqI GGGAC 3 cut(s) 646, 1180, 2127
FauI CCCGC 2 cut(s) 1256, 1415
FauNDI CATATG 1 cut(s) 2122
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 1974
Fnu4HI GCNGC 8 cut(s) 84, 549, 781, 1179, 1443, 2154, 2157, 2265
FokI GGATG 4 cut(s) 92, 806, 1664, 1838
FriOI GRGCYC 3 cut(s) 201, 368, 1408
Fsp4HI GCNGC 8 cut(s) 84, 549, 781, 1179, 1443, 2154, 2157, 2265
FspBI CTAG 7 cut(s) 1086, 1412, 1502, 1958, 2117, 2277, 2298
GlaI GCGC 2 cut(s) 668, 1625
GluI GCNGC 8 cut(s) 84, 549, 781, 1179, 1443, 2154, 2157, 2265
GsaI CCCAGC 2 cut(s) 166, 1201
GsuI CTGGAG 5 cut(s) 91, 194, 969, 1791, 2280
HaeIII GGCC 4 cut(s) 344, 1155, 1406, 1533
HapII CCGG 3 cut(s) 132, 369, 1587
HgaI GACGC 1 cut(s) 782
HhaI GCGC 2 cut(s) 669, 1626
Hin6I GCGC 2 cut(s) 667, 1624
HinP1I GCGC 2 cut(s) 667, 1624
HincII GTYRAC 2 cut(s) 318, 772
HindII GTYRAC 2 cut(s) 318, 772
HindIII AAGCTT 3 cut(s) 398, 588, 1788
HpaI GTTAAC 1 cut(s) 318
HpaII CCGG 3 cut(s) 132, 369, 1587
HphI GGTGA 4 cut(s) 489, 1237, 1347, 1676
Hpy166II GTNNAC 7 cut(s) 318, 772, 1466, 1852, 1975, 2097, 2128
Hpy8I GTNNAC 7 cut(s) 318, 772, 1466, 1852, 1975, 2097, 2128
HpyAV CCTTC 6 cut(s) 563, 1048, 1283, 1852, 1889, 2080
HpyCH4III ACNGT 4 cut(s) 391, 1574, 1688, 2321
HpyCH4IV ACGT 6 cut(s) 380, 688, 1077, 1230, 1387, 1672
HpySE526I ACGT 6 cut(s) 380, 688, 1077, 1230, 1387, 1672
HspAI GCGC 2 cut(s) 667, 1624
Kpn2I TCCGGA 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 787
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
LmnI GCTCC 6 cut(s) 116, 140, 371, 1147, 2261, 2379
Lsp1109I GCAGC 7 cut(s) 560, 767, 1190, 1429, 2140, 2168, 2276
LweI GCATC 7 cut(s) 70, 214, 812, 1349, 1556, 1816, 2131
MaeI CTAG 7 cut(s) 1086, 1412, 1502, 1958, 2117, 2277, 2298
MaeII ACGT 6 cut(s) 380, 688, 1077, 1230, 1387, 1672
MaeIII GTNAC 2 cut(s) 1383, 2362
MalI GATC 8 cut(s) 477, 721, 789, 954, 1475, 2212, 2275, 2406
MboI GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
MflI RGATCY 3 cut(s) 952, 2273, 2404
MhlI GDGCHC 9 cut(s) 201, 368, 541, 1299, 1408, 1854, 2247, 2305, 2384
MlsI TGGCCA 1 cut(s) 1155
MluNI TGGCCA 1 cut(s) 1155
MlyI GAGTC 4 cut(s) 122, 134, 423, 1266
MmeI TCCRAC 8 cut(s) 150, 162, 240, 709, 1093, 1629, 2038, 2357
Mox20I TGGCCA 1 cut(s) 1155
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 2034
MscI TGGCCA 1 cut(s) 1155
MseI TTAA 7 cut(s) 243, 317, 455, 711, 1262, 1709, 2328
MslI CAYNNNNRTG 1 cut(s) 1539
Msp20I TGGCCA 1 cut(s) 1155
MspA1I CMGCKG 3 cut(s) 86, 551, 1181
MspCI CTTAAG 1 cut(s) 1261
MspI CCGG 3 cut(s) 132, 369, 1587
MspR9I CCNGG 4 cut(s) 173, 1108, 1498, 2186
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 4 cut(s) 173, 1108, 1498, 2186
MvnI CGCG 1 cut(s) 38
NdeI CATATG 1 cut(s) 2122
NdeII GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
NlaIV GGNNCC 8 cut(s) 136, 367, 373, 763, 1406, 1921, 2352, 2406
NmeAIII GCCGAG 1 cut(s) 220
NspI RCATGY 2 cut(s) 563, 2452
NspV TTCGAA 1 cut(s) 2077
PagI TCATGA 1 cut(s) 918
PaqCI CACCTGC 1 cut(s) 508
PasI CCCWGGG 1 cut(s) 1107
PciI ACATGT 1 cut(s) 2448
PctI GAATGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 2034
PfeI GAWTC 7 cut(s) 116, 122, 178, 247, 491, 1526, 1724
PflMI CCANNNNNTGG 1 cut(s) 2225
PfoI TCCNGGA 1 cut(s) 2184
PkrI GCNGC 8 cut(s) 85, 550, 782, 1180, 1444, 2155, 2158, 2266
PleI GAGTC 4 cut(s) 122, 134, 422, 1266
PpsI GAGTC 4 cut(s) 122, 134, 422, 1266
Ppu21I YACGTR 1 cut(s) 1231
PscI ACATGT 1 cut(s) 2448
PsiI TTATAA 2 cut(s) 846, 1070
Psp124BI GAGCTC 1 cut(s) 201
Psp1406I AACGTT 1 cut(s) 1077
Psp6I CCWGG 4 cut(s) 171, 1106, 1496, 2184
PspFI CCCAGC 2 cut(s) 162, 1197
PspGI CCWGG 4 cut(s) 171, 1106, 1496, 2184
PspN4I GGNNCC 8 cut(s) 136, 367, 373, 763, 1406, 1921, 2352, 2406
PspOMI GGGCCC 1 cut(s) 1404
PspPI GGNCC 7 cut(s) 342, 371, 1404, 1405, 1531, 1551, 1713
PstI CTGCAG 3 cut(s) 550, 785, 2158
PsuI RGATCY 3 cut(s) 952, 2273, 2404
PvuII CAGCTG 2 cut(s) 551, 1181
RsaI GTAC 3 cut(s) 1229, 1483, 1932
RsaNI GTAC 3 cut(s) 1228, 1482, 1931
RseI CAYNNNNRTG 1 cut(s) 1539
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 7 cut(s) 243, 317, 455, 711, 1262, 1709, 2328
SatI GCNGC 8 cut(s) 84, 549, 781, 1179, 1443, 2154, 2157, 2265
Sau3AI GATC 8 cut(s) 475, 719, 787, 952, 1473, 2210, 2273, 2404
Sau96I GGNCC 7 cut(s) 342, 371, 1404, 1405, 1531, 1551, 1713
SchI GAGTC 4 cut(s) 122, 134, 423, 1266
ScrFI CCNGG 4 cut(s) 173, 1108, 1498, 2186
SduI GDGCHC 9 cut(s) 201, 368, 541, 1299, 1408, 1854, 2247, 2305, 2384
SfaNI GCATC 7 cut(s) 70, 214, 812, 1349, 1556, 1816, 2131
SfcI CTRYAG 6 cut(s) 387, 546, 781, 1002, 1570, 2154
SfuI TTCGAA 1 cut(s) 2077
SinI GGWCC 3 cut(s) 371, 1551, 1713
SmiMI CAYNNNNRTG 1 cut(s) 1539
SmlI CTYRAG 1 cut(s) 1261
SmoI CTYRAG 1 cut(s) 1261
SsiI CCGC 5 cut(s) 36, 84, 1249, 1408, 2348
SspMI CTAG 7 cut(s) 1086, 1412, 1502, 1958, 2117, 2277, 2298
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 4 cut(s) 171, 1106, 1496, 2184
StyI CCWWGG 6 cut(s) 345, 513, 1143, 1302, 1914, 2308
TaaI ACNGT 4 cut(s) 391, 1574, 1688, 2321
TaiI ACGT 6 cut(s) 383, 691, 1080, 1233, 1390, 1675
TaqI TCGA 5 cut(s) 189, 393, 466, 1659, 2077
TaqII GACCGA 1 cut(s) 1539
TatI WGTACW 1 cut(s) 1930
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 7 cut(s) 116, 122, 178, 247, 491, 1526, 1724
Tru1I TTAA 7 cut(s) 243, 317, 455, 711, 1262, 1709, 2328
Tru9I TTAA 7 cut(s) 243, 317, 455, 711, 1262, 1709, 2328
TscAI CASTG 8 cut(s) 790, 1483, 1597, 1996, 2056, 2104, 2275, 2326
TseI GCWGC 7 cut(s) 548, 780, 1178, 1442, 2153, 2156, 2264
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 8 cut(s) 790, 1483, 1597, 1996, 2056, 2104, 2275, 2326
Van91I CCANNNNNTGG 1 cut(s) 2225
Vha464I CTTAAG 1 cut(s) 1261
VneI GTGCAC 1 cut(s) 1850
VpaK11BI GGWCC 3 cut(s) 371, 1551, 1713
XapI RAATTY 6 cut(s) 457, 660, 840, 932, 1580, 1750
XbaI TCTAGA 1 cut(s) 1957
XceI RCATGY 2 cut(s) 563, 2452
XmiI GTMKAC 1 cut(s) 1974
XmnI GAANNNNTTC 1 cut(s) 2034
XspI CTAG 7 cut(s) 1086, 1412, 1502, 1958, 2117, 2277, 2298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.