Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003871

Physical Location & Seq

Genomic Coordinates Reverse (-)

4162927 .. 4167107

Gene Structure 4181 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1061000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGGTGTGCTGACATGCAACAGGTGGACCCGAGTTCTCCGGCGCCAGAACTCCCAGATCGACGACGCCGACAACAACCACAACTCCAACAACATCGAAGACATCATCAACGCCCCAATTATCAGCAACTCTGTCGAATGTTACGCCTGCACTCAGGTGGGGGTCCCAGCCTTCCATTCCACCAGCTGCGACGGCGCCCACCAGCCCCAGTGGGAGGCCTCCGCCGGCTCTTCCCTGATTCCCATCCAATCCCGCTCCGATCTCAAAAAAGGAATCTCCCGCCACCGCCCAGCCGGCCCCTTCGGCCCCGTCCTCGACCCCCGCAGCAAGCGCGTCCAGCGCTGGAACCGCGCGTTCCTCCTCGCACGTGGGATGGCGCTGGCCGTCGACCCGCTCTTCTTCTACGCCCTCTCTTTCGGAAGAAGCGGAACCCCTTGCCTCTACATGGACGGAGGGCTGGCAGCAATTGTCACCGTGCTCCGCACGAGCGTGGACGCCGTGCACCTGTGCCACCTGTGGTTGCAGTTCCGGCTGGCGTACGTATCGAGGGAGTCGCTGGTGGTCGGCTGCGGAAAGCTCGTGTGGGACCCACGTGCGATCTCCTACCACTACCTTCGATCTCTCAAAGGTTTCTGGTTCGACGCCTTCGTCATTCTCCCCGTTCCTCAGGCAGTATTTTGGCTGGTTCTTCCAAAATTGATCAAAGAAGAGAAGGTTAAGGTGATAATGACCATTCTCCTTTTAATTTTTTTGTTCCAATTCCTCCCAAAAGTCTACCACAGCATCTCCTTGATGAGAAGAATGCAAAAGGTCACAGGCTACATATTTGGCACCATTTGGTGGGGTTTTGGCCTCAACCTCATTGCCTACTTCATTGCTTCTCACGTTGCCGGGGGATGCTGGTATGTGCTCGCGATTCAGCGCGTAGCTTCGTGCCTAAGACAGCATTGTGACCAAAATGTCAATTGTAATCTCTCTCTGTCTTGCTCGGACGAAGTTTGCTACGAGTTTTTGTTACCGGCCGCCACGATTGGCAACCCTTGTGGAGGGAACACGACAACAAGCGTGATGAGAAAGCCACCACTCTGCTTGGATGTAAATGGCACATTCAACTATGGAATCTATCAGTGGGCTCTTCCTGTCATATCTAGCAACTCCCTTGCTATTAAGATTCTCTATCCCATATTCTGGGGCCTAATGACTCTCGGCACATTTGGGAATGATCTTGAACCTACAAGTCACTGGTTAGAAGTGATTTTCAGCATATGCATTGTGCTTAGTGGGTTGCTGCTCTTCACTTTGTTGATTGGAAACATTCAGGTATTTCTCCACGCCGTTATGGCAAAGAAAAGGAAGATGCAGTTGAGGTGCAGAGACATGGAATGGTGGATGAAGCGGAGGCAGTTGCCGTCTGGATTGAGACGACGAGTACGCCATTACGAAAGGCATAGATGGGTGACAATGGGGGGAGAAGATGAGATGGATTTGATCAAAGACTTGCCGGAAGGGCTCCGAAGGGAGATTAAGCGCCATTTATGCTTAGACCTCATAAAAAAGGTTCCCCTGTTCCACAACTTGGATGATCTTATTCTTGACAATATGTGTGATAGGGTTAGGCCACTGGTCTTCTCCAAAGATGAAAAGATAATTAGAGAAGGAGATCCTGTGCCGAAGATGCTATTTATCGTACGAGGGCGCATTAAGCGCACCCAAGGCCTAAGCAAAGGCATGGTGGGAACCAATGTGCTTGAACCGGGAGGGTTTTTTGGCGATGAGCTGCTCTCCTGGTGCCTTCGCCGGCCATTCATGGACCGCCTTCCAGCCTCATCCGCGACATTCACCTGCATTGAATCAACAGAAGCATTTGGCCTCAATGCAGGCGATCTGAGATACATGACGGATCACTTCCGCTACAAATTTGCCAACGAAAGGCTTAAGCGAACGGCGAGATACTACTCTTCAAATTGGAGAACATGGGCAGCGGTGAATATACAATTCGCTTGGCGCCGGTACAGGTTGAGGACTAATGGAGGTCCCGTGATTCCTGTGATTCCAAATGGAAGCTCTGAGAGGAAGCTGTTGCAGTATGCTGCAATGTTCTTGTCGCTTAGGCCACATGATCACCTTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

711

Amino Acids

81.19

Weight (kDa)

9.53

Isoelectric Point (pI)

52.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	155 - 440	1.1e-06	Ion transport protein
cNMP_binding	PF00027	542 - 631	2.6e-06	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15410 AT5G15410
fragaria_vesca	FvH4_6g47520 FvH4_6g47520 FvH4_7g26890
malus_domestica	MD07G1248200.v1.1 MD09G1061000.v1.1 MD17G1056400.v1.1
prunus_persica	Prupe.2G272100_v2.0.a1 Prupe.3G259100_v2.0.a1
pyrus_communis	pycom07g22190 pycom111g05130 pycom17g05550
rosa_chinensis	RchiOBHm_Chr1g0373561 RchiOBHm_Chr2g0166581
rosa_laevigata	RLG00000021630 RLG00000026815
rosa_multiflora	Rmu_sc0002860.1_g000003 Rmu_sc0008222.1_g000009
rosa_roxburghii	Rroxscaffold_2G00084740 Rroxscaffold_4G00284140
rosa_rugosa	Rorug01G0378300 Rorug02G0524300 Rorug02G0524400
rosa_samantha	Rh1AG387700 Rh1BG352000 Rh1CG364700 Rh1DG382400 Rh2AG590200 Rh2BG602300 Rh2CG573000 Rh2DG612900
rosa_wichuraiana	Rw1G034770 Rw2G049200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1850
AasI	GACNNNNNNGTC	2 cut(s)	647, 959
Acc36I	ACCTGC	1 cut(s)	1850
AccB1I	GGYRCC	5 cut(s)	42, 194, 830, 1788, 2004
AccB7I	CCANNNNNTGG	3 cut(s)	840, 1188, 1576
AccBSI	CCGCTC	2 cut(s)	255, 394
AccI	GTMKAC	2 cut(s)	387, 774
AccII	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
AclWI	GGATC	2 cut(s)	1655, 1908
AcoI	YGGCCR	3 cut(s)	381, 1020, 1799
AcsI	RAATTY	1 cut(s)	1916
AcvI	CACGTG	2 cut(s)	368, 593
AcyI	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
AfaI	GTAC	4 cut(s)	539, 1431, 1689, 2012
AfeI	AGCGCT	1 cut(s)	341
AfiI	CCNNNNNNNGG	7 cut(s)	214, 445, 840, 1046, 1188, 1576, 1929
AflII	CTTAAG	1 cut(s)	1934
AgsI	TTSAA	5 cut(s)	1111, 1229, 1751, 1850, 1962
AjnI	CCWGG	1 cut(s)	1784
AleI	CACNNNNGTG	2 cut(s)	155, 488
AluBI	AGCT	6 cut(s)	186, 577, 929, 1777, 2064, 2077
AluI	AGCT	6 cut(s)	186, 577, 929, 1777, 2064, 2077
Alw21I	GWGCWC	3 cut(s)	480, 504, 912
Alw26I	GTCTC	2 cut(s)	1368, 1414
Alw44I	GTGCAC	1 cut(s)	500
AlwI	GGATC	2 cut(s)	1655, 1908
Ama87I	CYCGRG	1 cut(s)	30
Aor51HI	AGCGCT	1 cut(s)	341
ApaLI	GTGCAC	1 cut(s)	500
ApeKI	GCWGC	8 cut(s)	186, 324, 461, 567, 1288, 1777, 1979, 2090
ApoI	RAATTY	1 cut(s)	1916
AspS9I	GGNCC	8 cut(s)	27, 163, 296, 305, 586, 1192, 1810, 2033
AsuC2I	CCSGG	2 cut(s)	892, 1755
AsuHPI	GGTGA	6 cut(s)	463, 733, 1468, 1831, 1996, 2114
AvaI	CYCGRG	1 cut(s)	30
AvaII	GGWCC	5 cut(s)	27, 163, 586, 1810, 2033
AxyI	CCTNAGG	1 cut(s)	666
BaeGI	GKGCMC	1 cut(s)	504
BanI	GGYRCC	5 cut(s)	42, 194, 830, 1788, 2004
BanII	GRGCYC	2 cut(s)	1135, 1512
BauI	CACGAG	2 cut(s)	484, 578
BbrPI	CACGTG	2 cut(s)	368, 593
BbsI	GAAGAC	2 cut(s)	105, 1618
Bbv12I	GWGCWC	3 cut(s)	480, 504, 912
BbvI	GCAGC	8 cut(s)	173, 336, 473, 554, 1275, 1764, 1991, 2077
BccI	CCATC	4 cut(s)	251, 367, 1446, 1474
BceAI	ACGGC	6 cut(s)	208, 368, 482, 1319, 1393, 1959
BcgI	CGANNNNNNTGC	2 cut(s)	115, 149
BciT130I	CCWGG	1 cut(s)	1786
BclI	TGATCA	3 cut(s)	699, 1488, 2119
BcnI	CCSGG	2 cut(s)	892, 1755
BcoDI	GTCTC	2 cut(s)	1368, 1414
BfaI	CTAG	1 cut(s)	1149
BfoI	RGCGCY	6 cut(s)	46, 198, 343, 380, 1531, 2008
BfrI	CTTAAG	1 cut(s)	1934
BfuAI	ACCTGC	1 cut(s)	1850
BglI	GCCNNNNNGGC	4 cut(s)	294, 303, 1340, 1507
BisI	GCNGC	9 cut(s)	187, 325, 462, 568, 1023, 1289, 1778, 1980, 2091
BlsI	GCNGC	9 cut(s)	188, 326, 463, 569, 1024, 1290, 1779, 1981, 2092
Bme1390I	CCNGG	3 cut(s)	892, 1755, 1786
Bme18I	GGWCC	5 cut(s)	27, 163, 586, 1810, 2033
BmeT110I	CYCGRG	1 cut(s)	30
BmgT120I	GGNCC	8 cut(s)	27, 163, 296, 305, 586, 1192, 1810, 2033
BmrFI	CCNGG	3 cut(s)	892, 1755, 1786
BmrI	ACTGGG	1 cut(s)	202
BmsI	GCATC	4 cut(s)	792, 887, 1347, 1665
BmuI	ACTGGG	1 cut(s)	202
BpiI	GAAGAC	2 cut(s)	105, 1618
BplI	GAGNNNNNCTC	2 cut(s)	1766, 1798
Bpu10I	CCTNAGC	2 cut(s)	1718, 2108
BpuMI	CCSGG	2 cut(s)	892, 1755
BsaAI	YACGTR	3 cut(s)	368, 541, 593
BsaBI	GATNNNNATC	1 cut(s)	242
BsaHI	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
BsaJI	CCNNGG	2 cut(s)	891, 1711
BsaXI	ACNNNNNCTCC	8 cut(s)	68, 98, 770, 800, 1650, 1680, 2022, 2052
Bsc4I	CCNNNNNNNGG	7 cut(s)	214, 445, 840, 1046, 1188, 1576, 1929
Bse118I	RCCGGY	5 cut(s)	224, 293, 1018, 1797, 2007
Bse1I	ACTGG	3 cut(s)	208, 1247, 1626
Bse21I	CCTNAGG	1 cut(s)	666
Bse3DI	GCAATG	3 cut(s)	861, 873, 2100
Bse8I	GATNNNNATC	1 cut(s)	242
BseBI	CCWGG	1 cut(s)	1786
BseDI	CCNNGG	2 cut(s)	891, 1711
BseGI	GGATG	7 cut(s)	243, 378, 902, 1099, 1395, 1585, 1826
BseJI	GATNNNNATC	1 cut(s)	242
BseLI	CCNNNNNNNGG	7 cut(s)	214, 445, 840, 1046, 1188, 1576, 1929
BseMI	GCAATG	3 cut(s)	861, 873, 2100
BseMII	CTCAG	4 cut(s)	167, 680, 1877, 2058
BseNI	ACTGG	3 cut(s)	208, 1247, 1626
BseRI	GAGGAG	1 cut(s)	350
BseSI	GKGCMC	1 cut(s)	504
BseX3I	CGGCCG	1 cut(s)	1020
BseXI	GCAGC	8 cut(s)	173, 336, 473, 554, 1275, 1764, 1991, 2077
BseYI	CCCAGC	2 cut(s)	166, 289
BsgI	GTGCAG	2 cut(s)	133, 1390
Bsh1236I	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
Bsh1285I	CGRYCG	1 cut(s)	1023
BshNI	GGYRCC	5 cut(s)	42, 194, 830, 1788, 2004
BsiEI	CGRYCG	1 cut(s)	1023
BsiHKAI	GWGCWC	3 cut(s)	480, 504, 912
BsiHKCI	CYCGRG	1 cut(s)	30
BsiWI	CGTACG	2 cut(s)	537, 1687
BslFI	GGGAC	3 cut(s)	149, 599, 2019
BslI	CCNNNNNNNGG	7 cut(s)	214, 445, 840, 1046, 1188, 1576, 1929
BsmAI	GTCTC	2 cut(s)	1368, 1414
BsmBI	CGTCTC	1 cut(s)	1414
BsmFI	GGGAC	3 cut(s)	149, 599, 2019
BsmI	GAATGC	1 cut(s)	807
BsoBI	CYCGRG	1 cut(s)	30
Bsp1286I	GDGCHC	5 cut(s)	480, 504, 912, 1135, 1512
Bsp68I	TCGCGA	1 cut(s)	914
BspCNI	CTCAG	4 cut(s)	166, 679, 1878, 2059
BspFNI	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
BspMI	ACCTGC	1 cut(s)	1850
BspPI	GGATC	2 cut(s)	1655, 1908
BspQI	GCTCTTC	4 cut(s)	235, 401, 1140, 1298
BspT107I	GGYRCC	5 cut(s)	42, 194, 830, 1788, 2004
BspTI	CTTAAG	1 cut(s)	1934
BsrBI	CCGCTC	2 cut(s)	255, 394
BsrDI	GCAATG	3 cut(s)	861, 873, 2100
BsrFI	RCCGGY	5 cut(s)	224, 293, 1018, 1797, 2007
BsrI	ACTGG	3 cut(s)	208, 1247, 1626
BssAI	RCCGGY	5 cut(s)	224, 293, 1018, 1797, 2007
BssECI	CCNNGG	2 cut(s)	891, 1711
BssNI	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
BssSI	CACGAG	2 cut(s)	484, 578
BssT1I	CCWWGG	1 cut(s)	1711
Bst2BI	CACGAG	2 cut(s)	484, 578
Bst2UI	CCWGG	1 cut(s)	1786
Bst4CI	ACNGT	1 cut(s)	475
Bst6I	CTCTTC	6 cut(s)	235, 401, 702, 1140, 1298, 1963
BstACI	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
BstAFI	CTTAAG	1 cut(s)	1934
BstBAI	YACGTR	3 cut(s)	368, 541, 593
BstDEI	CTNAG	9 cut(s)	153, 666, 938, 1277, 1540, 1718, 1886, 2067, 2108
BstENI	CCTNNNNNAGG	1 cut(s)	1044
BstF5I	GGATG	7 cut(s)	243, 378, 902, 1099, 1395, 1585, 1826
BstFNI	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
BstH2I	RGCGCY	6 cut(s)	46, 198, 343, 380, 1531, 2008
BstMAI	GTCTC	2 cut(s)	1368, 1414
BstMCI	CGRYCG	1 cut(s)	1023
BstNI	CCWGG	1 cut(s)	1786
BstNSI	RCATGY	1 cut(s)	18
BstSCI	CCNGG	3 cut(s)	890, 1753, 1784
BstSLI	GKGCMC	1 cut(s)	504
BstSNI	TACGTA	1 cut(s)	541
BstUI	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
BstV1I	GCAGC	8 cut(s)	173, 336, 473, 554, 1275, 1764, 1991, 2077
BstV2I	GAAGAC	2 cut(s)	105, 1618
BstX2I	RGATCY	1 cut(s)	1660
BstYI	RGATCY	1 cut(s)	1660
BstZI	CGGCCG	1 cut(s)	1020
Bsu36I	CCTNAGG	1 cut(s)	666
BtgZI	GCGATG	1 cut(s)	1785
BtsCI	GGATG	7 cut(s)	243, 378, 902, 1099, 1395, 1585, 1826
BtsIMutI	CAGTG	4 cut(s)	215, 1133, 1240, 1619
BtuMI	TCGCGA	1 cut(s)	914
BveI	ACCTGC	1 cut(s)	1850
Cfr10I	RCCGGY	5 cut(s)	224, 293, 1018, 1797, 2007
Cfr13I	GGNCC	8 cut(s)	27, 163, 296, 305, 586, 1192, 1810, 2033
CseI	GACGC	4 cut(s)	74, 322, 503, 650
Csp6I	GTAC	4 cut(s)	538, 1430, 1688, 2011
CspCI	CAANNNNNGTGG	4 cut(s)	1024, 1059, 1071, 1106
CviAII	CATG	8 cut(s)	15, 445, 1378, 1729, 1807, 1894, 1974, 2117
CviQI	GTAC	4 cut(s)	538, 1430, 1688, 2011
DdeI	CTNAG	9 cut(s)	153, 666, 938, 1277, 1540, 1718, 1886, 2067, 2108
DinI	GGCGCC	3 cut(s)	44, 196, 2006
DrdI	GACNNNNNNGTC	2 cut(s)	647, 959
DseDI	GACNNNNNNGTC	2 cut(s)	647, 959
EaeI	YGGCCR	3 cut(s)	381, 1020, 1799
EagI	CGGCCG	1 cut(s)	1020
Eam1104I	CTCTTC	6 cut(s)	235, 401, 702, 1140, 1298, 1963
EarI	CTCTTC	6 cut(s)	235, 401, 702, 1140, 1298, 1963
EciI	GGCGGA	1 cut(s)	211
EclXI	CGGCCG	1 cut(s)	1020
Eco105I	TACGTA	1 cut(s)	541
Eco130I	CCWWGG	1 cut(s)	1711
Eco147I	AGGCCT	2 cut(s)	218, 1716
Eco24I	GRGCYC	2 cut(s)	1135, 1512
Eco47I	GGWCC	5 cut(s)	27, 163, 586, 1810, 2033
Eco47III	AGCGCT	1 cut(s)	341
Eco52I	CGGCCG	1 cut(s)	1020
Eco72I	CACGTG	2 cut(s)	368, 593
Eco81I	CCTNAGG	1 cut(s)	666
Eco88I	CYCGRG	1 cut(s)	30
EcoNI	CCTNNNNNAGG	1 cut(s)	1044
EcoO109I	RGGNCCY	4 cut(s)	163, 586, 1192, 2033
EcoRII	CCWGG	1 cut(s)	1784
EcoT14I	CCWWGG	1 cut(s)	1711
EcoT22I	ATGCAT	1 cut(s)	1271
EcoT38I	GRGCYC	2 cut(s)	1135, 1512
EgeI	GGCGCC	3 cut(s)	44, 196, 2006
EheI	GGCGCC	3 cut(s)	44, 196, 2006
ErhI	CCWWGG	1 cut(s)	1711
Esp3I	CGTCTC	1 cut(s)	1414
FaeI	CATG	8 cut(s)	18, 448, 1381, 1732, 1810, 1897, 1977, 2120
FaqI	GGGAC	3 cut(s)	149, 599, 2019
FatI	CATG	8 cut(s)	14, 444, 1377, 1728, 1806, 1893, 1973, 2116
FauI	CCCGC	4 cut(s)	260, 287, 329, 399
FauNDI	CATATG	1 cut(s)	1265
FbaI	TGATCA	3 cut(s)	699, 1488, 2119
FblI	GTMKAC	2 cut(s)	387, 774
Fnu4HI	GCNGC	9 cut(s)	187, 325, 462, 568, 1023, 1289, 1778, 1980, 2091
FokI	GGATG	7 cut(s)	230, 385, 909, 1106, 1402, 1592, 1813
FriOI	GRGCYC	2 cut(s)	1135, 1512
Fsp4HI	GCNGC	9 cut(s)	187, 325, 462, 568, 1023, 1289, 1778, 1980, 2091
FspBI	CTAG	1 cut(s)	1149
GluI	GCNGC	9 cut(s)	187, 325, 462, 568, 1023, 1289, 1778, 1980, 2091
GsaI	CCCAGC	2 cut(s)	170, 293
HaeII	RGCGCY	6 cut(s)	46, 198, 343, 380, 1531, 2008
HgaI	GACGC	4 cut(s)	74, 322, 503, 650
Hin1I	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
Hin1II	CATG	8 cut(s)	18, 448, 1381, 1732, 1810, 1897, 1977, 2120
HincII	GTYRAC	1 cut(s)	388
HindII	GTYRAC	1 cut(s)	388
HphI	GGTGA	6 cut(s)	463, 733, 1468, 1831, 1996, 2114
Hpy166II	GTNNAC	5 cut(s)	27, 388, 493, 502, 775
Hpy188I	TCNGA	6 cut(s)	259, 419, 991, 1514, 1887, 2068
Hpy188III	TCNNGA	4 cut(s)	913, 1226, 1413, 1592
Hpy8I	GTNNAC	5 cut(s)	27, 388, 493, 502, 775
Hpy99I	CGWCG	6 cut(s)	65, 68, 194, 389, 644, 1428
HpyCH4III	ACNGT	1 cut(s)	475
HpyCH4IV	ACGT	4 cut(s)	367, 540, 592, 885
HpyF3I	CTNAG	9 cut(s)	153, 666, 938, 1277, 1540, 1718, 1886, 2067, 2108
HpySE526I	ACGT	4 cut(s)	367, 540, 592, 885
Hsp92I	GRCGYC	6 cut(s)	43, 66, 195, 495, 642, 2005
Hsp92II	CATG	8 cut(s)	18, 448, 1381, 1732, 1810, 1897, 1977, 2120
KasI	GGCGCC	3 cut(s)	42, 194, 2004
KflI	GGGWCCC	2 cut(s)	163, 586
KroI	GCCGGC	3 cut(s)	224, 293, 1797
KroNI	GCCGGC	3 cut(s)	226, 295, 1799
Ksp22I	TGATCA	3 cut(s)	699, 1488, 2119
LguI	GCTCTTC	4 cut(s)	235, 401, 1140, 1298
LmnI	GCTCC	3 cut(s)	260, 483, 1515
Lsp1109I	GCAGC	8 cut(s)	173, 336, 473, 554, 1275, 1764, 1991, 2077
LweI	GCATC	4 cut(s)	792, 887, 1347, 1665
MaeI	CTAG	1 cut(s)	1149
MaeII	ACGT	4 cut(s)	367, 540, 592, 885
MaeIII	GTNAC	7 cut(s)	140, 469, 811, 950, 1014, 1238, 1456
MbiI	CCGCTC	2 cut(s)	255, 394
MfeI	CAATTG	2 cut(s)	465, 964
MflI	RGATCY	1 cut(s)	1660
MhlI	GDGCHC	5 cut(s)	480, 504, 912, 1135, 1512
Mly113I	GGCGCC	3 cut(s)	43, 195, 2005
MlyI	GAGTC	2 cut(s)	560, 1195
MmeI	TCCRAC	1 cut(s)	111
Mph1103I	ATGCAT	1 cut(s)	1271
MroNI	GCCGGC	3 cut(s)	224, 293, 1797
MseI	TTAA	6 cut(s)	717, 743, 1167, 1524, 1701, 1935
MslI	CAYNNNNRTG	2 cut(s)	155, 488
MspA1I	CMGCKG	2 cut(s)	186, 1982
MspCI	CTTAAG	1 cut(s)	1934
MspR9I	CCNGG	3 cut(s)	892, 1755, 1786
MunI	CAATTG	2 cut(s)	465, 964
Mva1269I	GAATGC	1 cut(s)	807
MvaI	CCWGG	1 cut(s)	1786
MvnI	CGCG	6 cut(s)	333, 351, 353, 914, 924, 1832
NaeI	GCCGGC	3 cut(s)	226, 295, 1799
NarI	GGCGCC	3 cut(s)	43, 195, 2005
NciI	CCSGG	2 cut(s)	892, 1755
NdeI	CATATG	1 cut(s)	1265
NgoMIV	GCCGGC	3 cut(s)	224, 293, 1797
NlaIII	CATG	8 cut(s)	18, 448, 1381, 1732, 1810, 1897, 1977, 2120
NmeAIII	GCCGAG	1 cut(s)	1185
NmuCI	GTSAC	5 cut(s)	469, 811, 950, 1238, 1456
NruI	TCGCGA	1 cut(s)	914
NsiI	ATGCAT	1 cut(s)	1271
NspI	RCATGY	1 cut(s)	18
OliI	CACNNNNGTG	2 cut(s)	155, 488
PaqCI	CACCTGC	1 cut(s)	1850
PceI	AGGCCT	2 cut(s)	218, 1716
PciSI	GCTCTTC	4 cut(s)	235, 401, 1140, 1298
PcsI	WCGNNNNNNNCGW	2 cut(s)	66, 645
PctI	GAATGC	1 cut(s)	807
PdiI	GCCGGC	3 cut(s)	226, 295, 1799
PfeI	GAWTC	8 cut(s)	238, 273, 916, 1119, 1171, 1850, 2041, 2050
Pfl23II	CGTACG	2 cut(s)	537, 1687
PflMI	CCANNNNNTGG	3 cut(s)	840, 1188, 1576
PkrI	GCNGC	9 cut(s)	188, 326, 463, 569, 1024, 1290, 1779, 1981, 2092
PleI	GAGTC	2 cut(s)	559, 1195
PluTI	GGCGCC	3 cut(s)	46, 198, 2008
PmaCI	CACGTG	2 cut(s)	368, 593
PmlI	CACGTG	2 cut(s)	368, 593
PpsI	GAGTC	2 cut(s)	559, 1195
Ppu21I	YACGTR	3 cut(s)	368, 541, 593
PpuMI	RGGWCCY	3 cut(s)	163, 586, 2033
Psp5II	RGGWCCY	3 cut(s)	163, 586, 2033
Psp6I	CCWGG	1 cut(s)	1784
PspCI	CACGTG	2 cut(s)	368, 593
PspFI	CCCAGC	2 cut(s)	166, 289
PspGI	CCWGG	1 cut(s)	1784
PspLI	CGTACG	2 cut(s)	537, 1687
PspPI	GGNCC	8 cut(s)	27, 163, 296, 305, 586, 1192, 1810, 2033
PspPPI	RGGWCCY	3 cut(s)	163, 586, 2033
PsuI	RGATCY	1 cut(s)	1660
PvuII	CAGCTG	1 cut(s)	186
RruI	TCGCGA	1 cut(s)	914
RsaI	GTAC	4 cut(s)	539, 1431, 1689, 2012
RsaNI	GTAC	4 cut(s)	538, 1430, 1688, 2011
RseI	CAYNNNNRTG	2 cut(s)	155, 488
SalI	GTCGAC	1 cut(s)	386
SapI	GCTCTTC	4 cut(s)	235, 401, 1140, 1298
SaqAI	TTAA	6 cut(s)	717, 743, 1167, 1524, 1701, 1935
SatI	GCNGC	9 cut(s)	187, 325, 462, 568, 1023, 1289, 1778, 1980, 2091
Sau96I	GGNCC	8 cut(s)	27, 163, 296, 305, 586, 1192, 1810, 2033
SchI	GAGTC	2 cut(s)	560, 1195
ScrFI	CCNGG	3 cut(s)	892, 1755, 1786
SduI	GDGCHC	5 cut(s)	480, 504, 912, 1135, 1512
SfaNI	GCATC	4 cut(s)	792, 887, 1347, 1665
SfiI	GGCCNNNNNGGCC	1 cut(s)	303
SfoI	GGCGCC	3 cut(s)	44, 196, 2006
SinI	GGWCC	5 cut(s)	27, 163, 586, 1810, 2033
SmiMI	CAYNNNNRTG	2 cut(s)	155, 488
SmlI	CTYRAG	2 cut(s)	1934, 2126
SmoI	CTYRAG	2 cut(s)	1934, 2126
SnaBI	TACGTA	1 cut(s)	541
SseBI	AGGCCT	2 cut(s)	218, 1716
SspDI	GGCGCC	3 cut(s)	42, 194, 2004
SspMI	CTAG	1 cut(s)	1149
StuI	AGGCCT	2 cut(s)	218, 1716
StyD4I	CCNGG	3 cut(s)	890, 1753, 1784
StyI	CCWWGG	1 cut(s)	1711
TaaI	ACNGT	1 cut(s)	475
TaiI	ACGT	4 cut(s)	370, 543, 595, 888
TaqI	TCGA	8 cut(s)	60, 96, 135, 315, 387, 545, 616, 639
TauI	GCSGC	1 cut(s)	1025
TfiI	GAWTC	8 cut(s)	238, 273, 916, 1119, 1171, 1850, 2041, 2050
Tru1I	TTAA	6 cut(s)	717, 743, 1167, 1524, 1701, 1935
Tru9I	TTAA	6 cut(s)	717, 743, 1167, 1524, 1701, 1935
TscAI	CASTG	4 cut(s)	215, 1133, 1247, 1626
TseFI	GTSAC	5 cut(s)	469, 811, 950, 1238, 1456
TseI	GCWGC	8 cut(s)	186, 324, 461, 567, 1288, 1777, 1979, 2090
Tsp45I	GTSAC	5 cut(s)	469, 811, 950, 1238, 1456
TspDTI	ATGAA	4 cut(s)	862, 1406, 1653, 1795
TspGWI	ACGGA	2 cut(s)	465, 1913
TspRI	CASTG	4 cut(s)	215, 1133, 1247, 1626
Van91I	CCANNNNNTGG	3 cut(s)	840, 1188, 1576
Vha464I	CTTAAG	1 cut(s)	1934
VneI	GTGCAC	1 cut(s)	500
VpaK11BI	GGWCC	5 cut(s)	27, 163, 586, 1810, 2033
XagI	CCTNNNNNAGG	1 cut(s)	1044
XapI	RAATTY	1 cut(s)	1916
XceI	RCATGY	1 cut(s)	18
XmiI	GTMKAC	2 cut(s)	387, 774
XspI	CTAG	1 cut(s)	1149
Zsp2I	ATGCAT	1 cut(s)	1271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.