Rh2BG602300

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003871
Physical Location & Seq
Forward (+)
82242660 .. 82246510
3851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG602300.1

Sequence Viewer

Length: 1761 bp
ATGGCCCTCGCCATCGACCCGCTCTTCTTCTACGCCCTCTCCATCGGCCGCGGTGGGACTCCCTGCCTCTACATGGACGGCGGCCTTGCGGCAATCGTGACGGTGCTGCGCACATGCGTGGACGCCGTCCACCTTTGCCACCTGTGGCTTCAGTTCCGCCTGGCGTACGTTTCTAGGGAGTCGCTGGTGGTCGGCTGCGGGAAGCTCGTGTGGGACGCACGTGCGATAGCGTCGCACTATGTTCGGTCCCTCAAGGGCTTCTGGTTCGACGCCTTCGTTATTCTTCCGGTTCCTCAGGCTGTGTTCTGGCTTGTTCTTCCAAAATTGATCAGGGAAGAGAAGATTAAAGTGATAATGACAATTCTTCTAGTAATATTTTTATTCCAATTCCTCCCAAAAGTCTACCATAGCATCTGCTTGATGAGAAGAATGGCAAAGGTCACAGGCTACATTTTCGGCACCATTTGGTGGGGTTTTGGCCTCAACCTCATCGCCTACTTCATTGCCTCACATGTTGCTGGGGGATGCTGGTATGTACTTGCTATACAGCGAGTGGCTTCCTGCCTAAAGCAACAGTGTGAATTGGGTGGGAATTGCAATCTCTCTTTGTCATGCTCACAGGAAGTTTGTTACCAATCTCTGATACCGGATTCCATGGAAGCAACTCCATGTGGTAACAATGTGACAAATGTGTTGAGAAAGCCTTCCTGCTTGGATGTTAATGGAACATTCAACTACGGCATCTATCAGTGGGCTCTTCCTGTCATATCTAGCAACTCGCTGTCTATAAAGATCCTTTATCCTATATTTTGGGGCTTAATGACTCTCAGCACATTTGGCAATGATCTTGAACCAACAAGTCACTGGCTAGAAGTGATTTTCAGTATATGCATTGTGCTCAGTGGATTACTGCTCTTCACTTTGCTTATTGGGAACATTCAGGTATTTCTGCACGCGGTTATGGCAAAGAAGAGGAAAATGCAGCTGAGGTGCAGAGACATGGAATGGTGGATGAGAAGAAGGCAGTTGCCATCTGGTTTGCGACGAAGAGTCAGGCATTATGAACGCCATAGATGGGTGACAATGGGGGGAGAAGATGAGATGGATTTGATCAAAGACTTGCCTGAAGGTCTTCGAAGAGAAATCAAACGCCACTTATGCATAGACCTCATAAAGAAGGTACCTCTGTTCCACAACTTGGATGATCTGATTCTTGACAACATATGTGATCGCGTTAGGCCTCTGGTCTTTTCTAGAGATGAAAAGATAATTAGAGAAGGAGACCCTGTGCCAAAGATGCTATTCATTGTGCGTGGACGCATAAAGCGCAGCCAAGGCCTAAGTAAAGGCATGGTGGGAACCAATGTGCTTGAACCCGGTGGGTTTCTAGGCGATGAGCTGCTCTCCTGGTGCCTACGCCGGCCCTTCATAGACCGCCTTCCCGCCTCATCCGCCACATTTTCTTGCATTGAATCAACAGAAGCGTTCGGCCTCAATGCAAGCGACCTCAGATACATTACAGACCACTTCCGGTACAAATTTGCAAATGAGAGGCTTAAGAGAACTGCTAGATACTACTCTTCCAATTGGCGAACATGGGCTGCTGTAAATGTACAATTTGCTTGGCGCCGCTACAGGCTGAGGACCAGAGGTCCTGTGATTCCTGTATTGACCCCCGAACAAAATGGAGGCGCTGATCGTAAGCTCTTGCAGTATGCTGCATTGTTCATGTCACTTAGGCCGCATGATCATCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

586

Amino Acids

67.33

Weight (kDa)

9.47

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 31 - 314 7.1e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 110
Acc65I GGTACC 1 cut(s) 1180
AccB1I GGYRCC 4 cut(s) 458, 1180, 1410, 1626
AccB7I CCANNNNNTGG 2 cut(s) 468, 1198
AccBSI CCGCTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 402
AccII CGCG 3 cut(s) 51, 956, 1233
AclWI GGATC 1 cut(s) 787
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 1538
AcuI CTGAAG 2 cut(s) 134, 1146
AcvI CACGTG 1 cut(s) 221
AcyI GRCGYC 3 cut(s) 123, 270, 1627
AfaI GTAC 5 cut(s) 167, 537, 1182, 1535, 1614
AfiI CCNNNNNNNGG 4 cut(s) 73, 468, 1075, 1198
AflII CTTAAG 1 cut(s) 1556
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 4 cut(s) 733, 851, 1373, 1472
AhdI GACNNNNNGTC 2 cut(s) 1049, 1650
AjnI CCWGG 2 cut(s) 159, 1406
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 4 cut(s) 205, 985, 1399, 1705
AluI AGCT 4 cut(s) 205, 985, 1399, 1705
Alw21I GWGCWC 1 cut(s) 900
Alw26I GTCTC 2 cut(s) 990, 1275
AlwI GGATC 1 cut(s) 787
AoxI GGCC 9 cut(s) 3, 46, 82, 478, 1238, 1336, 1421, 1489, 1739
ApeKI GCWGC 7 cut(s) 106, 195, 982, 1329, 1399, 1601, 1718
ApoI RAATTY 1 cut(s) 1538
Asp700I GAANNNNTTC 2 cut(s) 703, 1131
Asp718I GGTACC 1 cut(s) 1180
AspLEI GCGC 4 cut(s) 111, 1329, 1629, 1694
AspS9I GGNCC 5 cut(s) 4, 246, 1422, 1644, 1652
AsuC2I CCSGG 1 cut(s) 1377
AsuHPI GGTGA 1 cut(s) 1090
AsuII TTCGAA 1 cut(s) 1135
AvaII GGWCC 3 cut(s) 246, 1644, 1652
AxyI CCTNAGG 1 cut(s) 294
BaeI ACNNNNGTAYC 2 cut(s) 1172, 1205
BanI GGYRCC 4 cut(s) 458, 1180, 1410, 1626
BanII GRGCYC 1 cut(s) 757
BauI CACGAG 1 cut(s) 206
BbrPI CACGTG 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 1124
Bbv12I GWGCWC 1 cut(s) 900
BbvCI CCTCAGC 2 cut(s) 986, 1640
BbvI GCAGC 7 cut(s) 93, 182, 994, 1341, 1386, 1588, 1705
BccI CCATC 5 cut(s) 20, 50, 1039, 1068, 1096
BceAI ACGGC 3 cut(s) 94, 110, 754
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 2 cut(s) 161, 1408
BclI TGATCA 3 cut(s) 327, 1110, 1747
BcnI CCSGG 1 cut(s) 1377
BcoDI GTCTC 2 cut(s) 990, 1275
BfaI CTAG 7 cut(s) 174, 368, 771, 869, 1254, 1388, 1569
BfmI CTRYAG 1 cut(s) 1633
BfoI RGCGCY 2 cut(s) 1630, 1695
BfrI CTTAAG 1 cut(s) 1556
Bme1390I CCNGG 3 cut(s) 161, 1377, 1408
Bme18I GGWCC 3 cut(s) 246, 1644, 1652
BmeRI GACNNNNNGTC 2 cut(s) 1049, 1650
BmgT120I GGNCC 5 cut(s) 4, 246, 1422, 1644, 1652
BmiI GGNNCC 7 cut(s) 248, 291, 460, 1182, 1360, 1412, 1628
BmrFI CCNGG 3 cut(s) 161, 1377, 1408
BmsI GCATC 4 cut(s) 420, 515, 750, 1287
BpiI GAAGAC 1 cut(s) 1124
BplI GAGNNNNNCTC 2 cut(s) 1388, 1420
Bpu10I CCTNAGC 2 cut(s) 986, 1640
Bpu14I TTCGAA 1 cut(s) 1135
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 1377
BsaAI YACGTR 1 cut(s) 221
BsaHI GRCGYC 3 cut(s) 123, 270, 1627
BsaI GGTCTC 1 cut(s) 1275
BsaJI CCNNGG 3 cut(s) 49, 654, 1333
BsaWI WCCGGW 3 cut(s) 286, 646, 1530
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 1272, 1302
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 468, 1075, 1198
Bse118I RCCGGY 1 cut(s) 1419
Bse1I ACTGG 1 cut(s) 869
Bse21I CCTNAGG 1 cut(s) 294
Bse3DI GCAATG 2 cut(s) 501, 847
BseBI CCWGG 2 cut(s) 161, 1408
BseDI CCNNGG 3 cut(s) 49, 654, 1333
BseGI GGATG 5 cut(s) 530, 721, 1017, 1207, 1448
BseLI CCNNNNNNNGG 4 cut(s) 73, 468, 1075, 1198
BseMI GCAATG 2 cut(s) 501, 847
BseMII CTCAG 6 cut(s) 308, 841, 913, 977, 1522, 1631
BseNI ACTGG 1 cut(s) 869
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 7 cut(s) 93, 182, 994, 1341, 1386, 1588, 1705
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 2 cut(s) 935, 1012
Bsh1236I CGCG 3 cut(s) 51, 956, 1233
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 9 cut(s) 5, 48, 84, 480, 1240, 1338, 1423, 1491, 1741
BshNI GGYRCC 4 cut(s) 458, 1180, 1410, 1626
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 900
BsiSI CCGG 5 cut(s) 287, 647, 1377, 1420, 1531
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 3 cut(s) 70, 227, 232
BslI CCNNNNNNNGG 4 cut(s) 73, 468, 1075, 1198
BsmAI GTCTC 2 cut(s) 990, 1275
BsmFI GGGAC 3 cut(s) 70, 227, 232
BsnI GGCC 9 cut(s) 5, 48, 84, 480, 1240, 1338, 1423, 1491, 1741
Bso31I GGTCTC 1 cut(s) 1275
Bsp119I TTCGAA 1 cut(s) 1135
Bsp1286I GDGCHC 2 cut(s) 757, 900
Bsp1407I TGTACA 1 cut(s) 1612
Bsp143I GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
Bsp19I CCATGG 1 cut(s) 654
BspANI GGCC 9 cut(s) 5, 48, 84, 480, 1240, 1338, 1423, 1491, 1741
BspCNI CTCAG 6 cut(s) 307, 840, 912, 978, 1521, 1632
BspFNI CGCG 3 cut(s) 51, 956, 1233
BspLI GGNNCC 7 cut(s) 248, 291, 460, 1182, 1360, 1412, 1628
BspPI GGATC 1 cut(s) 787
BspQI GCTCTTC 3 cut(s) 29, 762, 920
BspT104I TTCGAA 1 cut(s) 1135
BspT107I GGYRCC 4 cut(s) 458, 1180, 1410, 1626
BspTI CTTAAG 1 cut(s) 1556
BspTNI GGTCTC 1 cut(s) 1275
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 501, 847
BsrFI RCCGGY 1 cut(s) 1419
BsrGI TGTACA 1 cut(s) 1612
BsrI ACTGG 1 cut(s) 869
BssAI RCCGGY 1 cut(s) 1419
BssECI CCNNGG 3 cut(s) 49, 654, 1333
BssMI GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
BssNI GRCGYC 3 cut(s) 123, 270, 1627
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 654, 1333
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 161, 1408
Bst4CI ACNGT 2 cut(s) 103, 576
Bst6I CTCTTC 8 cut(s) 29, 330, 762, 920, 965, 1042, 1132, 1585
BstACI GRCGYC 3 cut(s) 123, 270, 1627
BstAFI CTTAAG 1 cut(s) 1556
BstAUI TGTACA 1 cut(s) 1612
BstBAI YACGTR 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 1135
BstC8I GCNNGC 3 cut(s) 954, 1421, 1501
BstDEI CTNAG 8 cut(s) 294, 827, 899, 986, 1340, 1508, 1640, 1736
BstDSI CCRYGG 2 cut(s) 49, 654
BstF5I GGATG 5 cut(s) 530, 721, 1017, 1207, 1448
BstFNI CGCG 3 cut(s) 51, 956, 1233
BstH2I RGCGCY 2 cut(s) 1630, 1695
BstHHI GCGC 4 cut(s) 111, 1329, 1629, 1694
BstKTI GATC 8 cut(s) 330, 795, 847, 1113, 1207, 1231, 1699, 1750
BstMAI GTCTC 2 cut(s) 990, 1275
BstMBI GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 7 cut(s) 837, 962, 1158, 1297, 1326, 1335, 1451
BstNI CCWGG 2 cut(s) 161, 1408
BstNSI RCATGY 2 cut(s) 117, 515
BstSCI CCNGG 3 cut(s) 159, 1375, 1406
BstSFI CTRYAG 1 cut(s) 1633
BstUI CGCG 3 cut(s) 51, 956, 1233
BstV1I GCAGC 7 cut(s) 93, 182, 994, 1341, 1386, 1588, 1705
BstV2I GAAGAC 1 cut(s) 1124
BstX2I RGATCY 1 cut(s) 792
BstYI RGATCY 1 cut(s) 792
BstZI CGGCCG 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 294
BsuRI GGCC 9 cut(s) 5, 48, 84, 480, 1240, 1338, 1423, 1491, 1741
BtgI CCRYGG 2 cut(s) 49, 654
BtgZI GCGATG 2 cut(s) 475, 1407
BtsCI GGATG 5 cut(s) 530, 721, 1017, 1207, 1448
BtsIMutI CAGTG 4 cut(s) 581, 755, 862, 907
Cac8I GCNNGC 3 cut(s) 954, 1421, 1501
CfoI GCGC 4 cut(s) 111, 1329, 1629, 1694
Cfr10I RCCGGY 1 cut(s) 1419
Cfr13I GGNCC 5 cut(s) 4, 246, 1422, 1644, 1652
Cfr42I CCGCGG 1 cut(s) 52
CseI GACGC 5 cut(s) 131, 219, 224, 278, 1326
Csp6I GTAC 5 cut(s) 166, 536, 1181, 1534, 1613
CviQI GTAC 5 cut(s) 166, 536, 1181, 1534, 1613
DdeI CTNAG 8 cut(s) 294, 827, 899, 986, 1340, 1508, 1640, 1736
DinI GGCGCC 1 cut(s) 1628
DpnI GATC 8 cut(s) 329, 794, 846, 1112, 1206, 1230, 1698, 1749
DpnII GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
DriI GACNNNNNGTC 2 cut(s) 1049, 1650
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
Eam1104I CTCTTC 8 cut(s) 29, 330, 762, 920, 965, 1042, 1132, 1585
Eam1105I GACNNNNNGTC 2 cut(s) 1049, 1650
EarI CTCTTC 8 cut(s) 29, 330, 762, 920, 965, 1042, 1132, 1585
EciI GGCGGA 2 cut(s) 146, 1441
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 654, 1333
Eco147I AGGCCT 2 cut(s) 1240, 1338
Eco24I GRGCYC 1 cut(s) 757
Eco31I GGTCTC 1 cut(s) 1275
Eco47I GGWCC 3 cut(s) 246, 1644, 1652
Eco52I CGGCCG 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 134, 1146
Eco72I CACGTG 1 cut(s) 221
Eco81I CCTNAGG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 1652
EcoRII CCWGG 2 cut(s) 159, 1406
EcoT14I CCWWGG 2 cut(s) 654, 1333
EcoT22I ATGCAT 2 cut(s) 893, 1163
EcoT38I GRGCYC 1 cut(s) 757
EgeI GGCGCC 1 cut(s) 1628
EheI GGCGCC 1 cut(s) 1628
ErhI CCWWGG 2 cut(s) 654, 1333
FaqI GGGAC 3 cut(s) 70, 227, 232
FauI CCCGC 3 cut(s) 27, 191, 1450
FauNDI CATATG 1 cut(s) 1223
FbaI TGATCA 3 cut(s) 327, 1110, 1747
FblI GTMKAC 1 cut(s) 402
FokI GGATG 5 cut(s) 537, 728, 1024, 1214, 1435
FriOI GRGCYC 1 cut(s) 757
FspBI CTAG 7 cut(s) 174, 368, 771, 869, 1254, 1388, 1569
FspI TGCGCA 1 cut(s) 110
GlaI GCGC 4 cut(s) 110, 1328, 1628, 1693
GsaI CCCAGC 1 cut(s) 522
HaeII RGCGCY 2 cut(s) 1630, 1695
HaeIII GGCC 9 cut(s) 5, 48, 84, 480, 1240, 1338, 1423, 1491, 1741
HapII CCGG 5 cut(s) 287, 647, 1377, 1420, 1531
HgaI GACGC 5 cut(s) 131, 219, 224, 278, 1326
HhaI GCGC 4 cut(s) 111, 1329, 1629, 1694
Hin1I GRCGYC 3 cut(s) 123, 270, 1627
Hin6I GCGC 4 cut(s) 109, 1327, 1627, 1692
HinP1I GCGC 4 cut(s) 109, 1327, 1627, 1692
HinfI GANTC 8 cut(s) 58, 179, 650, 823, 1050, 1210, 1472, 1660
HpaII CCGG 5 cut(s) 287, 647, 1377, 1420, 1531
HphI GGTGA 1 cut(s) 1090
Hpy166II GTNNAC 4 cut(s) 121, 130, 403, 1316
Hpy188I TCNGA 3 cut(s) 642, 1209, 1511
Hpy188III TCNNGA 5 cut(s) 97, 848, 1214, 1254, 1754
Hpy8I GTNNAC 4 cut(s) 121, 130, 403, 1316
Hpy99I CGWCG 3 cut(s) 235, 272, 1047
HpyAV CCTTC 8 cut(s) 283, 714, 1014, 1121, 1171, 1271, 1435, 1448
HpyCH4III ACNGT 2 cut(s) 103, 576
HpyCH4IV ACGT 2 cut(s) 168, 220
HpyF10VI GCNNNNNNNGC 7 cut(s) 837, 962, 1158, 1297, 1326, 1335, 1451
HpyF3I CTNAG 8 cut(s) 294, 827, 899, 986, 1340, 1508, 1640, 1736
HpySE526I ACGT 2 cut(s) 168, 220
Hsp92I GRCGYC 3 cut(s) 123, 270, 1627
HspAI GCGC 4 cut(s) 109, 1327, 1627, 1692
KasI GGCGCC 1 cut(s) 1626
KpnI GGTACC 1 cut(s) 1184
KroI GCCGGC 1 cut(s) 1419
KroNI GCCGGC 1 cut(s) 1421
Ksp22I TGATCA 3 cut(s) 327, 1110, 1747
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
LguI GCTCTTC 3 cut(s) 29, 762, 920
Lsp1109I GCAGC 7 cut(s) 93, 182, 994, 1341, 1386, 1588, 1705
LweI GCATC 4 cut(s) 420, 515, 750, 1287
MaeI CTAG 7 cut(s) 174, 368, 771, 869, 1254, 1388, 1569
MaeII ACGT 2 cut(s) 168, 220
MaeIII GTNAC 8 cut(s) 97, 439, 629, 674, 682, 860, 1078, 1731
MalI GATC 8 cut(s) 329, 794, 846, 1112, 1206, 1230, 1698, 1749
MbiI CCGCTC 1 cut(s) 22
MboI GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
MfeI CAATTG 1 cut(s) 1585
MflI RGATCY 1 cut(s) 792
MhlI GDGCHC 2 cut(s) 757, 900
MluCI AATT 9 cut(s) 323, 360, 386, 581, 592, 1269, 1538, 1585, 1616
Mly113I GGCGCC 1 cut(s) 1627
MlyI GAGTC 4 cut(s) 52, 188, 817, 1059
Mph1103I ATGCAT 2 cut(s) 893, 1163
MroNI GCCGGC 1 cut(s) 1419
MroXI GAANNNNTTC 2 cut(s) 703, 1131
MseI TTAA 4 cut(s) 345, 720, 818, 1557
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 51, 985
MspCI CTTAAG 1 cut(s) 1556
MspI CCGG 5 cut(s) 287, 647, 1377, 1420, 1531
MspR9I CCNGG 3 cut(s) 161, 1377, 1408
MunI CAATTG 1 cut(s) 1585
MvaI CCWGG 2 cut(s) 161, 1408
MvnI CGCG 3 cut(s) 51, 956, 1233
MwoI GCNNNNNNNGC 7 cut(s) 837, 962, 1158, 1297, 1326, 1335, 1451
NaeI GCCGGC 1 cut(s) 1421
NarI GGCGCC 1 cut(s) 1627
NciI CCSGG 1 cut(s) 1377
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1223
NdeII GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
NgoMIV GCCGGC 1 cut(s) 1419
NlaIV GGNNCC 7 cut(s) 248, 291, 460, 1182, 1360, 1412, 1628
NmuCI GTSAC 6 cut(s) 97, 439, 682, 860, 1078, 1731
NsbI TGCGCA 1 cut(s) 110
NsiI ATGCAT 2 cut(s) 893, 1163
NspI RCATGY 2 cut(s) 117, 515
NspV TTCGAA 1 cut(s) 1135
OliI CACNNNNGTG 1 cut(s) 116
PceI AGGCCT 2 cut(s) 1240, 1338
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 3 cut(s) 29, 762, 920
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 1421
PdmI GAANNNNTTC 2 cut(s) 703, 1131
PfeI GAWTC 4 cut(s) 650, 1210, 1472, 1660
Pfl23II CGTACG 1 cut(s) 165
PflFI GACNNNGTC 1 cut(s) 125
PflMI CCANNNNNTGG 2 cut(s) 468, 1198
PleI GAGTC 4 cut(s) 52, 187, 817, 1058
PluTI GGCGCC 1 cut(s) 1630
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
PpsI GAGTC 4 cut(s) 52, 187, 817, 1058
Ppu21I YACGTR 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 1652
PscI ACATGT 1 cut(s) 511
Psp5II RGGWCCY 1 cut(s) 1652
Psp6I CCWGG 2 cut(s) 159, 1406
PspCI CACGTG 1 cut(s) 221
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 2 cut(s) 159, 1406
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 7 cut(s) 248, 291, 460, 1182, 1360, 1412, 1628
PspPI GGNCC 5 cut(s) 4, 246, 1422, 1644, 1652
PspPPI RGGWCCY 1 cut(s) 1652
PsuI RGATCY 1 cut(s) 792
PsyI GACNNNGTC 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 985
RsaI GTAC 5 cut(s) 167, 537, 1182, 1535, 1614
RsaNI GTAC 5 cut(s) 166, 536, 1181, 1534, 1613
RseI CAYNNNNRTG 1 cut(s) 116
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 3 cut(s) 29, 762, 920
SaqAI TTAA 4 cut(s) 345, 720, 818, 1557
Sau3AI GATC 8 cut(s) 327, 792, 844, 1110, 1204, 1228, 1696, 1747
Sau96I GGNCC 5 cut(s) 4, 246, 1422, 1644, 1652
SchI GAGTC 4 cut(s) 52, 188, 817, 1059
ScrFI CCNGG 3 cut(s) 161, 1377, 1408
SduI GDGCHC 2 cut(s) 757, 900
SfaNI GCATC 4 cut(s) 420, 515, 750, 1287
SfcI CTRYAG 1 cut(s) 1633
SfoI GGCGCC 1 cut(s) 1628
Sfr303I CCGCGG 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 1135
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 3 cut(s) 246, 1644, 1652
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 3 cut(s) 251, 1556, 1754
SmoI CTYRAG 3 cut(s) 251, 1556, 1754
Sse9I AATT 9 cut(s) 323, 360, 386, 581, 592, 1269, 1538, 1585, 1616
SseBI AGGCCT 2 cut(s) 1240, 1338
SspDI GGCGCC 1 cut(s) 1626
SspI AATATT 1 cut(s) 375
SspMI CTAG 7 cut(s) 174, 368, 771, 869, 1254, 1388, 1569
StuI AGGCCT 2 cut(s) 1240, 1338
StyD4I CCNGG 3 cut(s) 159, 1375, 1406
StyI CCWWGG 2 cut(s) 654, 1333
TaaI ACNGT 2 cut(s) 103, 576
TaiI ACGT 2 cut(s) 171, 223
TaqI TCGA 3 cut(s) 15, 267, 1135
TaqII GACCGA 1 cut(s) 234
TasI AATT 9 cut(s) 323, 360, 386, 581, 592, 1269, 1538, 1585, 1616
TatI WGTACW 2 cut(s) 535, 1612
TauI GCSGC 5 cut(s) 51, 84, 92, 1632, 1744
TfiI GAWTC 4 cut(s) 650, 1210, 1472, 1660
Tru1I TTAA 4 cut(s) 345, 720, 818, 1557
Tru9I TTAA 4 cut(s) 345, 720, 818, 1557
TscAI CASTG 4 cut(s) 581, 755, 869, 907
TseFI GTSAC 6 cut(s) 97, 439, 682, 860, 1078, 1731
TseI GCWGC 7 cut(s) 106, 195, 982, 1329, 1399, 1601, 1718
Tsp45I GTSAC 6 cut(s) 97, 439, 682, 860, 1078, 1731
TspDTI ATGAA 6 cut(s) 490, 1077, 1275, 1294, 1417, 1717
TspRI CASTG 4 cut(s) 581, 755, 869, 907
Tth111I GACNNNGTC 1 cut(s) 125
Van91I CCANNNNNTGG 2 cut(s) 468, 1198
Vha464I CTTAAG 1 cut(s) 1556
VpaK11BI GGWCC 3 cut(s) 246, 1644, 1652
XapI RAATTY 1 cut(s) 1538
XbaI TCTAGA 1 cut(s) 1253
XceI RCATGY 2 cut(s) 117, 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 861
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 2 cut(s) 703, 1131
XspI CTAG 7 cut(s) 174, 368, 771, 869, 1254, 1388, 1569
Zsp2I ATGCAT 2 cut(s) 893, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.