Rroxscaffold_2G00084740

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003871
Physical Location & Seq
Reverse (-)
7240522 .. 7244246
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00084740.1

Sequence Viewer

Length: 1545 bp
ATGTCCTCCAACTTCTCCCTCCCAAGGTTTCATGCCCTCTACTCATTTATCGTATCTCGTTTAATTAAAATTCGAGGACGGGCTTTCCGAAGTGCTGGTCTCTTCTTCTGTAGACGCTTCAGGTGGACGGTATTCCACCGCCGGCAGAACTCACAGAGCGGCGGCGGCCCGAGCGACAGCGACAACAGCAACAACTTTGTGGAAGACAACCCAATCTCACTCTCGACCTCGGTGGTCGAGTGCTACGCGTGCACTCAGGTCGGAGTTCCGGTCTTCCACTCGACCAGCTGTGACGGCGCCCACCAGCCCCAATGGGAGGCCTCGGCGGGGTCTTCCCTAGTCCCTATCCAATCCCGGAGCGACCTCAAGAAGGGTCTCCGCCGCAACCGCCTCCCGGTGGGCCCCTTCGGCGAGGTTCTCGACCCGCGCACCAAGCGCGTGCAGAGGTGGAACCGCGCCTTCCTCCTGGCGCGTGGGATGGCGCTCGCCATCGACCCTCTCTTCTTCTACGCCCTCTCCATCGGCCGCGGTGGGACTCCCTGCCTCTACATGGACGGTGGCCTTGCGGCAATCGTCACGGTGCTCCGCACGTGCGTGGACGCCGTCCACCTTTGCCACCTGTGGCTTCAGTTCCGCCTGGCGTACGTTTCTAGAGAGTCGCTGGTGGTCGGCTGCGGGAAGCTCGTGTGGGACGCCCGTGCGATAGCGTCGCACTATGTTCGGTCTCTCAAGGGCTTCTGGTTCGACGCCTTCGTTATTCTTCCGGTTCCTCAGGCTGTGTTCTGGCTTGTTCTTCCAAAATTGATCAGGGAAGAGAAGATTAAAGTGATAATGACAATTCTTCTAGTAATATTTTTATTCCAATTCCTCCCAAAAGTCTACCATAGCATCTGCTTGATGAGAAGAATGGCAAAGGTCACAGGGTACATTTTCGGCACCATTTGGTGGGGTTTTGGCCTCAACCTCATCGCCTACTTCATTGCCTCACATGTTGCTGGGGGATGCTGGTATGTACTTGCTATACAGCGAGTGGCTTCCTGCTTAAAGCAACAGTGTGAATTGGGTGGGAATTGCAATCTCTCCTTGTCATGCTCACAGGAAGTTTGTTACCAATCTCTGATACCGGATTCCATGGAAGCAAGTCCATGTGGTAACAATGTCACAAATGTGTTGAGAAAGCCTTCCTGCTTGGATGTTAATGGAACATTCAACTACGGCATCTATCAGTGGGCTCTTCCTGTCATATCTAGCAACTCGCTGGCTATAAAGATCCTTTATCCTATATTTTGGGGCTTAATGACTCTCAGCACATTTGGCAATGATCTTGAACCAACAAGTCACTGGCTAGAAGTGATTTTCAGTATATGCATTGTGCTCAGTGGATTACTGCTCTTCACTTTGCTTATTGGGAACATTCAGGTGAGTATTTCTGCACGCGGTTATGGCAAAGAAGAGGAAAATGCAGCTGAGGTGCAGAGACATGGAATGGTGGATGAGAAGAAGGCAGTTGCCATCTGGTTTGCGACGAAGAGTCAGGCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

514

Amino Acids

57.46

Weight (kDa)

9.27

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 190 - 477 1e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 296, 935
AccB7I CCANNNNNTGG 1 cut(s) 945
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 2 cut(s) 112, 879
AccII CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
AclWI GGATC 1 cut(s) 1264
AcoI YGGCCR 1 cut(s) 523
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 2 cut(s) 103, 611
AcvI CACGTG 1 cut(s) 591
AcyI GRCGYC 4 cut(s) 297, 600, 693, 747
AfaI GTAC 3 cut(s) 644, 926, 1014
AfiI CCNNNNNNNGG 8 cut(s) 24, 316, 327, 370, 394, 397, 550, 945
AflIII ACRYGT 2 cut(s) 246, 988
AgsI TTSAA 2 cut(s) 1210, 1328
AhdI GACNNNNNGTC 1 cut(s) 1530
AjnI CCWGG 2 cut(s) 465, 636
AleI CACNNNNGTG 2 cut(s) 593, 1166
AluBI AGCT 3 cut(s) 288, 682, 1466
AluI AGCT 3 cut(s) 288, 682, 1466
Alw21I GWGCWC 3 cut(s) 254, 585, 1377
Alw26I GTCTC 4 cut(s) 104, 380, 729, 1471
Alw44I GTGCAC 1 cut(s) 250
AlwI GGATC 1 cut(s) 1264
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 6 cut(s) 166, 318, 400, 523, 559, 955
ApaI GGGCCC 1 cut(s) 404
ApaLI GTGCAC 1 cut(s) 250
ApeKI GCWGC 2 cut(s) 672, 1463
ApoI RAATTY 1 cut(s) 69
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
Asp700I GAANNNNTTC 1 cut(s) 1180
AspLEI GCGC 6 cut(s) 299, 429, 438, 458, 472, 484
AspS9I GGNCC 3 cut(s) 167, 400, 401
AsuC2I CCSGG 2 cut(s) 355, 395
AsuHPI GGTGA 1 cut(s) 1432
AvaI CYCGRG 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 771
BaeGI GKGCMC 2 cut(s) 254, 404
BanI GGYRCC 2 cut(s) 296, 935
BanII GRGCYC 2 cut(s) 404, 1234
BauI CACGAG 1 cut(s) 683
BbrPI CACGTG 1 cut(s) 591
BbsI GAAGAC 3 cut(s) 210, 265, 324
Bbv12I GWGCWC 3 cut(s) 254, 585, 1377
BbvCI CCTCAGC 1 cut(s) 1467
BbvI GCAGC 2 cut(s) 659, 1475
BccI CCATC 4 cut(s) 472, 497, 527, 1520
BceAI ACGGC 3 cut(s) 310, 587, 1231
BcgI CGANNNNNNTGC 4 cut(s) 241, 275, 701, 735
BciT130I CCWGG 2 cut(s) 467, 638
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 2 cut(s) 355, 395
BcoDI GTCTC 4 cut(s) 104, 380, 729, 1471
BfaI CTAG 5 cut(s) 338, 651, 845, 1248, 1346
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 2 cut(s) 300, 485
BglI GCCNNNNNGGC 1 cut(s) 408
BisI GCNGC 8 cut(s) 160, 163, 166, 382, 526, 567, 673, 1464
BlsI GCNGC 8 cut(s) 161, 164, 167, 383, 527, 568, 674, 1465
Bme1390I CCNGG 4 cut(s) 355, 395, 467, 638
BmeRI GACNNNNNGTC 1 cut(s) 1530
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 167, 400, 401
BmiI GGNNCC 6 cut(s) 298, 402, 403, 452, 768, 937
BmrFI CCNGG 4 cut(s) 355, 395, 467, 638
BmsI GCATC 3 cut(s) 897, 992, 1227
BpiI GAAGAC 3 cut(s) 210, 265, 324
Bpu10I CCTNAGC 1 cut(s) 1467
BpuEI CTTGAG 2 cut(s) 350, 713
BpuMI CCSGG 2 cut(s) 355, 395
BsaAI YACGTR 1 cut(s) 591
BsaHI GRCGYC 4 cut(s) 297, 600, 693, 747
BsaI GGTCTC 3 cut(s) 104, 380, 729
BsaJI CCNNGG 5 cut(s) 23, 228, 321, 526, 1131
BsaWI WCCGGW 3 cut(s) 268, 763, 1123
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 500, 530
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 316, 327, 370, 394, 397, 550, 945
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 1346
Bse21I CCTNAGG 1 cut(s) 771
Bse3DI GCAATG 2 cut(s) 978, 1324
BseBI CCWGG 2 cut(s) 467, 638
BseDI CCNNGG 5 cut(s) 23, 228, 321, 526, 1131
BseGI GGATG 4 cut(s) 483, 1007, 1198, 1498
BseLI CCNNNNNNNGG 8 cut(s) 24, 316, 327, 370, 394, 397, 550, 945
BseMI GCAATG 2 cut(s) 978, 1324
BseMII CTCAG 5 cut(s) 269, 785, 1318, 1390, 1458
BseNI ACTGG 1 cut(s) 1346
BseSI GKGCMC 2 cut(s) 254, 404
BseX3I CGGCCG 1 cut(s) 523
BseXI GCAGC 2 cut(s) 659, 1475
BseYI CCCAGC 1 cut(s) 995
BsgI GTGCAG 3 cut(s) 461, 1416, 1493
Bsh1236I CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 6 cut(s) 168, 320, 402, 525, 561, 957
BshNI GGYRCC 2 cut(s) 296, 935
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 3 cut(s) 254, 585, 1377
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 6 cut(s) 142, 269, 355, 395, 764, 1124
BsiWI CGTACG 1 cut(s) 642
BslFI GGGAC 3 cut(s) 326, 547, 704
BslI CCNNNNNNNGG 8 cut(s) 24, 316, 327, 370, 394, 397, 550, 945
BsmAI GTCTC 4 cut(s) 104, 380, 729, 1471
BsmFI GGGAC 3 cut(s) 326, 547, 704
BsnI GGCC 6 cut(s) 168, 320, 402, 525, 561, 957
Bso31I GGTCTC 3 cut(s) 104, 380, 729
BsoBI CYCGRG 1 cut(s) 169
Bsp120I GGGCCC 1 cut(s) 400
Bsp1286I GDGCHC 5 cut(s) 254, 404, 585, 1234, 1377
Bsp143I GATC 3 cut(s) 804, 1269, 1321
Bsp19I CCATGG 1 cut(s) 1131
BspANI GGCC 6 cut(s) 168, 320, 402, 525, 561, 957
BspCNI CTCAG 5 cut(s) 268, 784, 1317, 1389, 1459
BspFNI CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
BspLI GGNNCC 6 cut(s) 298, 402, 403, 452, 768, 937
BspPI GGATC 1 cut(s) 1264
BspQI GCTCTTC 2 cut(s) 1239, 1397
BspT107I GGYRCC 2 cut(s) 296, 935
BspTNI GGTCTC 3 cut(s) 104, 380, 729
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 2 cut(s) 978, 1324
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 1 cut(s) 1346
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 5 cut(s) 23, 228, 321, 526, 1131
BssMI GATC 3 cut(s) 804, 1269, 1321
BssNI GRCGYC 4 cut(s) 297, 600, 693, 747
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 23, 1131
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 467, 638
Bst4CI ACNGT 4 cut(s) 130, 557, 580, 1053
Bst6I CTCTTC 7 cut(s) 107, 506, 807, 1239, 1397, 1446, 1523
BstACI GRCGYC 4 cut(s) 297, 600, 693, 747
BstBAI YACGTR 1 cut(s) 591
BstC8I GCNNGC 6 cut(s) 143, 250, 440, 486, 1260, 1435
BstDEI CTNAG 5 cut(s) 255, 771, 1304, 1376, 1467
BstDSI CCRYGG 2 cut(s) 526, 1131
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 4 cut(s) 483, 1007, 1198, 1498
BstFNI CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
BstH2I RGCGCY 2 cut(s) 300, 485
BstHHI GCGC 6 cut(s) 299, 429, 438, 458, 472, 484
BstKTI GATC 3 cut(s) 807, 1272, 1324
BstMAI GTCTC 4 cut(s) 104, 380, 729, 1471
BstMBI GATC 3 cut(s) 804, 1269, 1321
BstMCI CGRYCG 1 cut(s) 526
BstNI CCWGG 2 cut(s) 467, 638
BstNSI RCATGY 1 cut(s) 992
BstSCI CCNGG 4 cut(s) 353, 393, 465, 636
BstSFI CTRYAG 1 cut(s) 109
BstSLI GKGCMC 2 cut(s) 254, 404
BstUI CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
BstV1I GCAGC 2 cut(s) 659, 1475
BstV2I GAAGAC 3 cut(s) 210, 265, 324
BstX2I RGATCY 1 cut(s) 1269
BstYI RGATCY 1 cut(s) 1269
BstZI CGGCCG 1 cut(s) 523
Bsu36I CCTNAGG 1 cut(s) 771
BsuRI GGCC 6 cut(s) 168, 320, 402, 525, 561, 957
BtgI CCRYGG 2 cut(s) 526, 1131
BtgZI GCGATG 1 cut(s) 952
BtsCI GGATG 4 cut(s) 483, 1007, 1198, 1498
BtsIMutI CAGTG 4 cut(s) 1058, 1232, 1339, 1384
Cac8I GCNNGC 6 cut(s) 143, 250, 440, 486, 1260, 1435
CfoI GCGC 6 cut(s) 299, 429, 438, 458, 472, 484
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 167, 400, 401
Cfr42I CCGCGG 1 cut(s) 529
CseI GACGC 5 cut(s) 123, 608, 696, 701, 755
Csp6I GTAC 3 cut(s) 643, 925, 1013
CviAII CATG 7 cut(s) 32, 550, 989, 1089, 1132, 1146, 1481
CviQI GTAC 3 cut(s) 643, 925, 1013
DdeI CTNAG 5 cut(s) 255, 771, 1304, 1376, 1467
DinI GGCGCC 1 cut(s) 298
DpnI GATC 3 cut(s) 806, 1271, 1323
DpnII GATC 3 cut(s) 804, 1269, 1321
DriI GACNNNNNGTC 1 cut(s) 1530
EaeI YGGCCR 1 cut(s) 523
EagI CGGCCG 1 cut(s) 523
Eam1104I CTCTTC 7 cut(s) 107, 506, 807, 1239, 1397, 1446, 1523
Eam1105I GACNNNNNGTC 1 cut(s) 1530
EarI CTCTTC 7 cut(s) 107, 506, 807, 1239, 1397, 1446, 1523
EciI GGCGGA 2 cut(s) 368, 623
EclXI CGGCCG 1 cut(s) 523
Eco130I CCWWGG 2 cut(s) 23, 1131
Eco147I AGGCCT 1 cut(s) 320
Eco24I GRGCYC 2 cut(s) 404, 1234
Eco31I GGTCTC 3 cut(s) 104, 380, 729
Eco52I CGGCCG 1 cut(s) 523
Eco57I CTGAAG 2 cut(s) 103, 611
Eco72I CACGTG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 169
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 465, 636
EcoT14I CCWWGG 2 cut(s) 23, 1131
EcoT22I ATGCAT 1 cut(s) 1370
EcoT38I GRGCYC 2 cut(s) 404, 1234
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 2 cut(s) 23, 1131
FaeI CATG 7 cut(s) 35, 553, 992, 1092, 1135, 1149, 1484
FaqI GGGAC 3 cut(s) 326, 547, 704
FatI CATG 7 cut(s) 31, 549, 988, 1088, 1131, 1145, 1480
FauI CCCGC 3 cut(s) 319, 432, 668
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 2 cut(s) 112, 879
Fnu4HI GCNGC 8 cut(s) 160, 163, 166, 382, 526, 567, 673, 1464
FokI GGATG 4 cut(s) 490, 1014, 1205, 1505
FriOI GRGCYC 2 cut(s) 404, 1234
Fsp4HI GCNGC 8 cut(s) 160, 163, 166, 382, 526, 567, 673, 1464
FspBI CTAG 5 cut(s) 338, 651, 845, 1248, 1346
GlaI GCGC 6 cut(s) 298, 428, 437, 457, 471, 483
GluI GCNGC 8 cut(s) 160, 163, 166, 382, 526, 567, 673, 1464
GsaI CCCAGC 1 cut(s) 999
HaeII RGCGCY 2 cut(s) 300, 485
HaeIII GGCC 6 cut(s) 168, 320, 402, 525, 561, 957
HapII CCGG 6 cut(s) 142, 269, 355, 395, 764, 1124
HgaI GACGC 5 cut(s) 123, 608, 696, 701, 755
HhaI GCGC 6 cut(s) 299, 429, 438, 458, 472, 484
Hin1I GRCGYC 4 cut(s) 297, 600, 693, 747
Hin1II CATG 7 cut(s) 35, 553, 992, 1092, 1135, 1149, 1484
Hin6I GCGC 6 cut(s) 297, 427, 436, 456, 470, 482
HinP1I GCGC 6 cut(s) 297, 427, 436, 456, 470, 482
HinfI GANTC 5 cut(s) 535, 656, 1127, 1300, 1531
HpaII CCGG 6 cut(s) 142, 269, 355, 395, 764, 1124
HphI GGTGA 1 cut(s) 1432
Hpy166II GTNNAC 6 cut(s) 113, 126, 252, 598, 607, 880
Hpy188I TCNGA 3 cut(s) 89, 263, 1119
Hpy188III TCNNGA 5 cut(s) 223, 367, 419, 651, 1325
Hpy8I GTNNAC 6 cut(s) 113, 126, 252, 598, 607, 880
Hpy99I CGWCG 3 cut(s) 712, 749, 1528
HpyAV CCTTC 6 cut(s) 364, 415, 469, 760, 1191, 1495
HpyCH4III ACNGT 4 cut(s) 130, 557, 580, 1053
HpyCH4IV ACGT 2 cut(s) 590, 645
HpyCH4V TGCA 7 cut(s) 252, 442, 1074, 1368, 1433, 1463, 1474
HpyF3I CTNAG 5 cut(s) 255, 771, 1304, 1376, 1467
HpySE526I ACGT 2 cut(s) 590, 645
Hsp92I GRCGYC 4 cut(s) 297, 600, 693, 747
Hsp92II CATG 7 cut(s) 35, 553, 992, 1092, 1135, 1149, 1484
HspAI GCGC 6 cut(s) 297, 427, 436, 456, 470, 482
KasI GGCGCC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 804
KspI CCGCGG 1 cut(s) 529
Kzo9I GATC 3 cut(s) 804, 1269, 1321
LguI GCTCTTC 2 cut(s) 1239, 1397
LmnI GCTCC 2 cut(s) 357, 588
Lsp1109I GCAGC 2 cut(s) 659, 1475
LweI GCATC 3 cut(s) 897, 992, 1227
MaeI CTAG 5 cut(s) 338, 651, 845, 1248, 1346
MaeII ACGT 2 cut(s) 590, 645
MaeIII GTNAC 7 cut(s) 290, 574, 916, 1106, 1151, 1159, 1337
MalI GATC 3 cut(s) 806, 1271, 1323
MbiI CCGCTC 1 cut(s) 159
MboI GATC 3 cut(s) 804, 1269, 1321
MflI RGATCY 1 cut(s) 1269
MhlI GDGCHC 5 cut(s) 254, 404, 585, 1234, 1377
MluCI AATT 7 cut(s) 63, 69, 800, 837, 863, 1058, 1069
MluI ACGCGT 1 cut(s) 246
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 4 cut(s) 529, 665, 1294, 1540
MmeI TCCRAC 2 cut(s) 33, 241
Mph1103I ATGCAT 1 cut(s) 1370
MroNI GCCGGC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 1180
MseI TTAA 6 cut(s) 62, 66, 822, 1043, 1197, 1295
MslI CAYNNNNRTG 3 cut(s) 593, 1166, 1418
MspA1I CMGCKG 3 cut(s) 288, 528, 1466
MspI CCGG 6 cut(s) 142, 269, 355, 395, 764, 1124
MspR9I CCNGG 4 cut(s) 355, 395, 467, 638
MvaI CCWGG 2 cut(s) 467, 638
MvnI CGCG 7 cut(s) 248, 427, 438, 456, 472, 528, 1437
NaeI GCCGGC 1 cut(s) 143
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 2 cut(s) 355, 395
NcoI CCATGG 1 cut(s) 1131
NdeII GATC 3 cut(s) 804, 1269, 1321
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 7 cut(s) 35, 553, 992, 1092, 1135, 1149, 1484
NlaIV GGNNCC 6 cut(s) 298, 402, 403, 452, 768, 937
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 5 cut(s) 290, 574, 916, 1159, 1337
NsiI ATGCAT 1 cut(s) 1370
NspI RCATGY 1 cut(s) 992
OliI CACNNNNGTG 2 cut(s) 593, 1166
PacI TTAATTAA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 320
PciI ACATGT 1 cut(s) 988
PciSI GCTCTTC 2 cut(s) 1239, 1397
PcsI WCGNNNNNNNCGW 2 cut(s) 85, 750
PdiI GCCGGC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 1180
PfeI GAWTC 1 cut(s) 1127
Pfl23II CGTACG 1 cut(s) 642
PflFI GACNNNGTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 945
PfoI TCCNGGA 1 cut(s) 353
PkrI GCNGC 8 cut(s) 161, 164, 167, 383, 527, 568, 674, 1465
PleI GAGTC 4 cut(s) 529, 664, 1294, 1539
PluTI GGCGCC 1 cut(s) 300
PmaCI CACGTG 1 cut(s) 591
PmlI CACGTG 1 cut(s) 591
PpsI GAGTC 4 cut(s) 529, 664, 1294, 1539
Ppu21I YACGTR 1 cut(s) 591
PscI ACATGT 1 cut(s) 988
Psp6I CCWGG 2 cut(s) 465, 636
PspCI CACGTG 1 cut(s) 591
PspFI CCCAGC 1 cut(s) 995
PspGI CCWGG 2 cut(s) 465, 636
PspLI CGTACG 1 cut(s) 642
PspN4I GGNNCC 6 cut(s) 298, 402, 403, 452, 768, 937
PspOMI GGGCCC 1 cut(s) 400
PspPI GGNCC 3 cut(s) 167, 400, 401
PsuI RGATCY 1 cut(s) 1269
PsyI GACNNNGTC 1 cut(s) 602
PvuII CAGCTG 2 cut(s) 288, 1466
RsaI GTAC 3 cut(s) 644, 926, 1014
RsaNI GTAC 3 cut(s) 643, 925, 1013
RseI CAYNNNNRTG 3 cut(s) 593, 1166, 1418
SacII CCGCGG 1 cut(s) 529
SapI GCTCTTC 2 cut(s) 1239, 1397
SaqAI TTAA 6 cut(s) 62, 66, 822, 1043, 1197, 1295
SatI GCNGC 8 cut(s) 160, 163, 166, 382, 526, 567, 673, 1464
Sau3AI GATC 3 cut(s) 804, 1269, 1321
Sau96I GGNCC 3 cut(s) 167, 400, 401
SchI GAGTC 4 cut(s) 529, 665, 1294, 1540
ScrFI CCNGG 4 cut(s) 355, 395, 467, 638
SduI GDGCHC 5 cut(s) 254, 404, 585, 1234, 1377
SfaNI GCATC 3 cut(s) 897, 992, 1227
SfcI CTRYAG 1 cut(s) 109
SfoI GGCGCC 1 cut(s) 298
Sfr303I CCGCGG 1 cut(s) 529
SgrBI CCGCGG 1 cut(s) 529
SmiMI CAYNNNNRTG 3 cut(s) 593, 1166, 1418
SmlI CTYRAG 2 cut(s) 365, 728
SmoI CTYRAG 2 cut(s) 365, 728
Sse9I AATT 7 cut(s) 63, 69, 800, 837, 863, 1058, 1069
SseBI AGGCCT 1 cut(s) 320
SspDI GGCGCC 1 cut(s) 296
SspI AATATT 1 cut(s) 852
SspMI CTAG 5 cut(s) 338, 651, 845, 1248, 1346
StuI AGGCCT 1 cut(s) 320
StyD4I CCNGG 4 cut(s) 353, 393, 465, 636
StyI CCWWGG 2 cut(s) 23, 1131
TaaI ACNGT 4 cut(s) 130, 557, 580, 1053
TaiI ACGT 2 cut(s) 593, 648
TaqI TCGA 7 cut(s) 73, 224, 237, 281, 420, 492, 744
TaqII GACCGA 1 cut(s) 711
TasI AATT 7 cut(s) 63, 69, 800, 837, 863, 1058, 1069
TatI WGTACW 1 cut(s) 1012
TauI GCSGC 6 cut(s) 162, 165, 168, 384, 528, 569
TfiI GAWTC 1 cut(s) 1127
Tru1I TTAA 6 cut(s) 62, 66, 822, 1043, 1197, 1295
Tru9I TTAA 6 cut(s) 62, 66, 822, 1043, 1197, 1295
TscAI CASTG 4 cut(s) 1058, 1232, 1346, 1384
TseFI GTSAC 5 cut(s) 290, 574, 916, 1159, 1337
TseI GCWGC 2 cut(s) 672, 1463
Tsp45I GTSAC 5 cut(s) 290, 574, 916, 1159, 1337
TspDTI ATGAA 2 cut(s) 20, 967
TspRI CASTG 4 cut(s) 1058, 1232, 1346, 1384
Tth111I GACNNNGTC 1 cut(s) 602
Van91I CCANNNNNTGG 1 cut(s) 945
VneI GTGCAC 1 cut(s) 250
XagI CCTNNNNNAGG 1 cut(s) 368
XapI RAATTY 1 cut(s) 69
XbaI TCTAGA 1 cut(s) 650
XceI RCATGY 1 cut(s) 992
XcmI CCANNNNNNNNNTGG 1 cut(s) 1338
XmiI GTMKAC 2 cut(s) 112, 879
XmnI GAANNNNTTC 1 cut(s) 1180
XspI CTAG 5 cut(s) 338, 651, 845, 1248, 1346
Zsp2I ATGCAT 1 cut(s) 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.