RH2AG590200

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0003871
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG590200.1

Sequence Viewer

Length: 2142 bp
ATGTCCTCCAACTTCTCCCTCCCAAGGTGGACGGTATTCCACCGCCGGCAGAACTCACAGAGCGGCGGCGGCCCGAGTGACAGCGACAACAGCAACAACTTTGTGGAAGACAACCCAATCTCACTCTCGACCTCGGTGATCGAGTGCTACGCGTGCACTCAGGTCGGAGTTCCGGTCTTCCACTCGACCAGCTGCGACGGCGCCCACCAGCCCCAATGGGAGGCCTCGGCGGGGTCTTCCCTAGTCCCTATCCAATCCCGGAGCGACCTCAAGAAGGGTCTCCGCCGCAACCGCCTCCCGGTGGGCCCCTTCGGCGAGGTCCTCGACCCGCGCACCAAGCGCGTGCAGAGGTGGAACCGCGCCTTCCTCCTGGCGCGTGGGTTGGCCCTCGCCATCGACCCGCTCTTCTTCTACGCCCTCTCCATCGGCCGCGGTGGGACTCCCTGCCTCTACATGGACGGCGGCCTTGCAGCAATCGTGACGGTGCTGCGCACGTGCGTGGACGCCGTCCACCTTTGCCACCTGTGGCTTCAGTTCCGCCTGGCGTACGTTTCTAGGGAGTCGCTGGTGGTCGGCTGCGGGAAGCTCGTGTGGGACGCACGTGCGATAGCGTCGCACTATGTTCGGTCCCTCAAGGGCTTCTGGTTCGACGCCTTCGTTATTCTTCCGGTTCCTCAGGCTGTGTTCTGGCTTGTTCTTCCAAAATTGATCAGGGAAGAGAAGATTAAAGTGATAATGACAATTCTTCTAGTAATATTTTTATTCCAATTCCTCCCAAAAGTCTACCATAGTATCTGCTTGATGAGAAGAATGGCAAAGGTCACTGGGTACATTTTCGGCACCATTTGGTGGGGTTTTGGCCTCAACCTCATCGCCTACTTCATTGCCTCACATGTTGCTGGGGGATGCTGGTATGTACTTGCTATACAGCGAGTGGCTTCCTGCCTAAAGCAACAGTGTGAATTGGGTGGGAATTGCAATCTCTCTTTGTCATGCTCACAGGAAGTTTGTTACCAATCTCTGATACCGGATTCCATGGAAGCAACTCCATGTGGTAACAATGTGACAAATGTGTTGAGAAAGCCTTCCTGCTTGGATGTTAATGGAACATTCAACTACGGCATCTATCAGTGGGCTCTTCCTGTCATATCTAGCAACTCGCTGGCTATAAAGATCCTTTATCCTATATTTTGGGGCTTAATGACTCTCAGCACATTTGGCAATGATCTTGAACCAACGAGTCACTGGCTAGAAGTGATTTTCAGTATATGCATTGTGCTCAGTGGATTACTGCTCTTCACTTTGCTTATTGGGAACATTCAGGTATTTCTGCACGCGGTTATGGCAAAGAAGAGGAAAATGCAGCTGAGGTGCAGAGACATGGAATGGTGGATGAGAAGAAGGCAGTTGCCATCTGGTTTGCGACGAAGAGTCAGGCATTATGAACGCCATAGATGGGTGACAATGGGGGGAGAAGATGAGATGGATTTGATCAAAGACTTGCCTGAAGGTCTTCGAAGAGAAATCAAACGCCACTTATGCATAGACCTCATAAAGAAGGTACCTCTGTTCCACAACTTGGATGATCTGATTCTTGACAACATATGTGATCGCGTTAGGCCTCTGGTCTTTTCTAGAGATGAAAAGATAATTAGAGAAGGAGACCCTGTGCCAAAGATGCTATTCATTGTGCGTGGACGCATAAAGCGCAGCCAAGGCCTAAGTAAAGGCATGGTGGGAACCAATGTGCTTGAACCCGGTGGGTTTCTAGGCGATGAGCTGCTCTCCTGGTGCCTACGCCGGCCCTTCATAGACCGCCTTCCCGCCTCATCCGCCACATTTTCTTGCATTGAATCAACAGAAGCGTTCGGCCTCAATGCAAGTGACCTCAGATACATTACAGACCACTTCCGGTACAAATTTGCAAATGAGAGGCTTAAGAGAACTGCAAGATACTACTCTTCCAATTGGCGAACATGGGCTGCTGTAAATGTACAATTTGCTTGGCGCCGCTACAGGCTGAGGACCAGAGGTCCTGTGATTCCTGTATTGACCCCCGAACAAAATGGAGGCGCTGATCGTAAGCTCTTGCAGTATGCTGCATTGTTCATGTCACTTAGGCCGCATGATCATCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

713

Amino Acids

81.32

Weight (kDa)

9.53

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 158 - 441 3.3e-06 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
Acc65I GGTACC 1 cut(s) 1561
AccB1I GGYRCC 5 cut(s) 200, 839, 1561, 1791, 2007
AccB7I CCANNNNNTGG 2 cut(s) 849, 1579
AccBSI CCGCTC 2 cut(s) 63, 403
AccI GTMKAC 1 cut(s) 783
AccII CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
AclWI GGATC 1 cut(s) 1168
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 1 cut(s) 1919
AcuI CTGAAG 2 cut(s) 515, 1527
AcvI CACGTG 2 cut(s) 495, 602
AcyI GRCGYC 4 cut(s) 201, 504, 651, 2008
AfaI GTAC 6 cut(s) 548, 830, 918, 1563, 1916, 1995
AflII CTTAAG 1 cut(s) 1937
AflIII ACRYGT 2 cut(s) 150, 892
AgsI TTSAA 4 cut(s) 1114, 1232, 1754, 1853
AhdI GACNNNNNGTC 2 cut(s) 1430, 2031
AjnI CCWGG 3 cut(s) 369, 540, 1787
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 5 cut(s) 192, 586, 1366, 1780, 2086
AluI AGCT 5 cut(s) 192, 586, 1366, 1780, 2086
Alw21I GWGCWC 2 cut(s) 158, 1281
Alw26I GTCTC 3 cut(s) 284, 1371, 1656
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 1 cut(s) 1168
Ama87I CYCGRG 1 cut(s) 73
ApaI GGGCCC 1 cut(s) 308
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 9 cut(s) 192, 470, 487, 576, 1363, 1710, 1780, 1982, 2099
ApoI RAATTY 1 cut(s) 1919
Asp700I GAANNNNTTC 2 cut(s) 1084, 1512
Asp718I GGTACC 1 cut(s) 1561
AspLEI GCGC 9 cut(s) 203, 333, 342, 362, 376, 492, 1710, 2010, 2075
AspS9I GGNCC 9 cut(s) 71, 304, 305, 319, 385, 627, 1803, 2025, 2033
AsuC2I CCSGG 3 cut(s) 259, 299, 1758
AsuHPI GGTGA 2 cut(s) 148, 1471
AsuII TTCGAA 1 cut(s) 1516
AvaI CYCGRG 1 cut(s) 73
AvaII GGWCC 4 cut(s) 319, 627, 2025, 2033
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 2 cut(s) 158, 308
BaeI ACNNNNGTAYC 4 cut(s) 775, 808, 1553, 1586
BanI GGYRCC 5 cut(s) 200, 839, 1561, 1791, 2007
BanII GRGCYC 2 cut(s) 308, 1138
BauI CACGAG 1 cut(s) 587
BbrPI CACGTG 2 cut(s) 495, 602
BbsI GAAGAC 4 cut(s) 114, 169, 228, 1505
Bbv12I GWGCWC 2 cut(s) 158, 1281
BbvCI CCTCAGC 2 cut(s) 1367, 2021
BbvI GCAGC 9 cut(s) 179, 474, 482, 563, 1375, 1722, 1767, 1969, 2086
BccI CCATC 5 cut(s) 401, 431, 1420, 1449, 1477
BceAI ACGGC 4 cut(s) 214, 475, 491, 1135
BcgI CGANNNNNNTGC 4 cut(s) 145, 179, 605, 639
BciT130I CCWGG 3 cut(s) 371, 542, 1789
BclI TGATCA 3 cut(s) 708, 1491, 2128
BcnI CCSGG 3 cut(s) 259, 299, 1758
BcoDI GTCTC 3 cut(s) 284, 1371, 1656
BfaI CTAG 7 cut(s) 242, 555, 749, 1152, 1250, 1635, 1769
BfmI CTRYAG 1 cut(s) 2014
BfoI RGCGCY 3 cut(s) 204, 2011, 2076
BfrI CTTAAG 1 cut(s) 1937
BglI GCCNNNNNGGC 1 cut(s) 312
Bme1390I CCNGG 6 cut(s) 259, 299, 371, 542, 1758, 1789
Bme18I GGWCC 4 cut(s) 319, 627, 2025, 2033
BmeRI GACNNNNNGTC 2 cut(s) 1430, 2031
BmeT110I CYCGRG 1 cut(s) 73
BmgT120I GGNCC 9 cut(s) 71, 304, 305, 319, 385, 627, 1803, 2025, 2033
BmrFI CCNGG 6 cut(s) 259, 299, 371, 542, 1758, 1789
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 3 cut(s) 896, 1131, 1668
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 4 cut(s) 114, 169, 228, 1505
BplI GAGNNNNNCTC 2 cut(s) 1769, 1801
Bpu10I CCTNAGC 2 cut(s) 1367, 2021
Bpu14I TTCGAA 1 cut(s) 1516
BpuEI CTTGAG 2 cut(s) 254, 617
BpuMI CCSGG 3 cut(s) 259, 299, 1758
BsaAI YACGTR 2 cut(s) 495, 602
BsaHI GRCGYC 4 cut(s) 201, 504, 651, 2008
BsaI GGTCTC 2 cut(s) 284, 1656
BsaJI CCNNGG 6 cut(s) 23, 132, 225, 430, 1035, 1714
BsaWI WCCGGW 4 cut(s) 172, 667, 1027, 1911
BsaXI ACNNNNNCTCC 6 cut(s) 159, 189, 404, 434, 1653, 1683
Bse118I RCCGGY 2 cut(s) 45, 1800
Bse1I ACTGG 2 cut(s) 829, 1250
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 2 cut(s) 882, 1228
BseBI CCWGG 3 cut(s) 371, 542, 1789
BseDI CCNNGG 6 cut(s) 23, 132, 225, 430, 1035, 1714
BseGI GGATG 5 cut(s) 911, 1102, 1398, 1588, 1829
BseMI GCAATG 2 cut(s) 882, 1228
BseMII CTCAG 7 cut(s) 173, 689, 1222, 1294, 1358, 1903, 2012
BseNI ACTGG 2 cut(s) 829, 1250
BseSI GKGCMC 2 cut(s) 158, 308
BseX3I CGGCCG 1 cut(s) 427
BseXI GCAGC 9 cut(s) 179, 474, 482, 563, 1375, 1722, 1767, 1969, 2086
BseYI CCCAGC 1 cut(s) 899
BsgI GTGCAG 3 cut(s) 365, 1316, 1393
Bsh1236I CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
Bsh1285I CGRYCG 1 cut(s) 430
BshNI GGYRCC 5 cut(s) 200, 839, 1561, 1791, 2007
BsiEI CGRYCG 1 cut(s) 430
BsiHKAI GWGCWC 2 cut(s) 158, 1281
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 9 cut(s) 46, 173, 259, 299, 668, 1028, 1758, 1801, 1912
BsiWI CGTACG 1 cut(s) 546
BslFI GGGAC 4 cut(s) 230, 451, 608, 613
BsmAI GTCTC 3 cut(s) 284, 1371, 1656
BsmFI GGGAC 4 cut(s) 230, 451, 608, 613
Bso31I GGTCTC 2 cut(s) 284, 1656
BsoBI CYCGRG 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 1516
Bsp120I GGGCCC 1 cut(s) 304
Bsp1286I GDGCHC 4 cut(s) 158, 308, 1138, 1281
Bsp1407I TGTACA 1 cut(s) 1993
Bsp143I GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
Bsp19I CCATGG 1 cut(s) 1035
BspCNI CTCAG 7 cut(s) 172, 688, 1221, 1293, 1359, 1902, 2013
BspFNI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BspPI GGATC 1 cut(s) 1168
BspQI GCTCTTC 3 cut(s) 410, 1143, 1301
BspT104I TTCGAA 1 cut(s) 1516
BspT107I GGYRCC 5 cut(s) 200, 839, 1561, 1791, 2007
BspTI CTTAAG 1 cut(s) 1937
BspTNI GGTCTC 2 cut(s) 284, 1656
BsrBI CCGCTC 2 cut(s) 63, 403
BsrDI GCAATG 2 cut(s) 882, 1228
BsrFI RCCGGY 2 cut(s) 45, 1800
BsrGI TGTACA 1 cut(s) 1993
BsrI ACTGG 2 cut(s) 829, 1250
BssAI RCCGGY 2 cut(s) 45, 1800
BssECI CCNNGG 6 cut(s) 23, 132, 225, 430, 1035, 1714
BssMI GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
BssNI GRCGYC 4 cut(s) 201, 504, 651, 2008
BssSI CACGAG 1 cut(s) 587
BssT1I CCWWGG 3 cut(s) 23, 1035, 1714
Bst2BI CACGAG 1 cut(s) 587
Bst2UI CCWGG 3 cut(s) 371, 542, 1789
Bst4CI ACNGT 3 cut(s) 34, 484, 957
Bst6I CTCTTC 8 cut(s) 410, 711, 1143, 1301, 1346, 1423, 1513, 1966
BstACI GRCGYC 4 cut(s) 201, 504, 651, 2008
BstAFI CTTAAG 1 cut(s) 1937
BstAUI TGTACA 1 cut(s) 1993
BstBAI YACGTR 2 cut(s) 495, 602
BstBI TTCGAA 1 cut(s) 1516
BstC8I GCNNGC 6 cut(s) 47, 154, 344, 1164, 1335, 1802
BstDEI CTNAG 9 cut(s) 159, 675, 1208, 1280, 1367, 1721, 1889, 2021, 2117
BstDSI CCRYGG 2 cut(s) 430, 1035
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 5 cut(s) 911, 1102, 1398, 1588, 1829
BstFNI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BstH2I RGCGCY 3 cut(s) 204, 2011, 2076
BstHHI GCGC 9 cut(s) 203, 333, 342, 362, 376, 492, 1710, 2010, 2075
BstKTI GATC 9 cut(s) 141, 711, 1176, 1228, 1494, 1588, 1612, 2080, 2131
BstMAI GTCTC 3 cut(s) 284, 1371, 1656
BstMBI GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
BstMCI CGRYCG 1 cut(s) 430
BstNI CCWGG 3 cut(s) 371, 542, 1789
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 6 cut(s) 257, 297, 369, 540, 1756, 1787
BstSFI CTRYAG 1 cut(s) 2014
BstSLI GKGCMC 2 cut(s) 158, 308
BstUI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
BstV1I GCAGC 9 cut(s) 179, 474, 482, 563, 1375, 1722, 1767, 1969, 2086
BstV2I GAAGAC 4 cut(s) 114, 169, 228, 1505
BstX2I RGATCY 1 cut(s) 1173
BstYI RGATCY 1 cut(s) 1173
BstZI CGGCCG 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 675
BtgI CCRYGG 2 cut(s) 430, 1035
BtgZI GCGATG 2 cut(s) 856, 1788
BtsCI GGATG 5 cut(s) 911, 1102, 1398, 1588, 1829
BtsIMutI CAGTG 5 cut(s) 822, 962, 1136, 1243, 1288
Cac8I GCNNGC 6 cut(s) 47, 154, 344, 1164, 1335, 1802
CfoI GCGC 9 cut(s) 203, 333, 342, 362, 376, 492, 1710, 2010, 2075
Cfr10I RCCGGY 2 cut(s) 45, 1800
Cfr13I GGNCC 9 cut(s) 71, 304, 305, 319, 385, 627, 1803, 2025, 2033
Cfr42I CCGCGG 1 cut(s) 433
CseI GACGC 5 cut(s) 512, 600, 605, 659, 1707
Csp6I GTAC 6 cut(s) 547, 829, 917, 1562, 1915, 1994
CviQI GTAC 6 cut(s) 547, 829, 917, 1562, 1915, 1994
DdeI CTNAG 9 cut(s) 159, 675, 1208, 1280, 1367, 1721, 1889, 2021, 2117
DinI GGCGCC 2 cut(s) 202, 2009
DpnI GATC 9 cut(s) 140, 710, 1175, 1227, 1493, 1587, 1611, 2079, 2130
DpnII GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
DriI GACNNNNNGTC 2 cut(s) 1430, 2031
EaeI YGGCCR 1 cut(s) 427
EagI CGGCCG 1 cut(s) 427
Eam1104I CTCTTC 8 cut(s) 410, 711, 1143, 1301, 1346, 1423, 1513, 1966
Eam1105I GACNNNNNGTC 2 cut(s) 1430, 2031
EarI CTCTTC 8 cut(s) 410, 711, 1143, 1301, 1346, 1423, 1513, 1966
EciI GGCGGA 3 cut(s) 272, 527, 1822
EclXI CGGCCG 1 cut(s) 427
Eco130I CCWWGG 3 cut(s) 23, 1035, 1714
Eco147I AGGCCT 3 cut(s) 224, 1621, 1719
Eco24I GRGCYC 2 cut(s) 308, 1138
Eco31I GGTCTC 2 cut(s) 284, 1656
Eco47I GGWCC 4 cut(s) 319, 627, 2025, 2033
Eco52I CGGCCG 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 515, 1527
Eco72I CACGTG 2 cut(s) 495, 602
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 73
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 3 cut(s) 305, 319, 2033
EcoRII CCWGG 3 cut(s) 369, 540, 1787
EcoT14I CCWWGG 3 cut(s) 23, 1035, 1714
EcoT22I ATGCAT 2 cut(s) 1274, 1544
EcoT38I GRGCYC 2 cut(s) 308, 1138
EgeI GGCGCC 2 cut(s) 202, 2009
EheI GGCGCC 2 cut(s) 202, 2009
ErhI CCWWGG 3 cut(s) 23, 1035, 1714
FaqI GGGAC 4 cut(s) 230, 451, 608, 613
FauI CCCGC 5 cut(s) 223, 336, 408, 572, 1831
FauNDI CATATG 1 cut(s) 1604
FbaI TGATCA 3 cut(s) 708, 1491, 2128
FblI GTMKAC 1 cut(s) 783
FokI GGATG 5 cut(s) 918, 1109, 1405, 1595, 1816
FriOI GRGCYC 2 cut(s) 308, 1138
FspBI CTAG 7 cut(s) 242, 555, 749, 1152, 1250, 1635, 1769
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 9 cut(s) 202, 332, 341, 361, 375, 491, 1709, 2009, 2074
GsaI CCCAGC 1 cut(s) 903
HaeII RGCGCY 3 cut(s) 204, 2011, 2076
HapII CCGG 9 cut(s) 46, 173, 259, 299, 668, 1028, 1758, 1801, 1912
HgaI GACGC 5 cut(s) 512, 600, 605, 659, 1707
HhaI GCGC 9 cut(s) 203, 333, 342, 362, 376, 492, 1710, 2010, 2075
Hin1I GRCGYC 4 cut(s) 201, 504, 651, 2008
Hin6I GCGC 9 cut(s) 201, 331, 340, 360, 374, 490, 1708, 2008, 2073
HinP1I GCGC 9 cut(s) 201, 331, 340, 360, 374, 490, 1708, 2008, 2073
HinfI GANTC 9 cut(s) 439, 560, 1031, 1204, 1240, 1431, 1591, 1853, 2041
HpaII CCGG 9 cut(s) 46, 173, 259, 299, 668, 1028, 1758, 1801, 1912
HphI GGTGA 2 cut(s) 148, 1471
Hpy166II GTNNAC 6 cut(s) 30, 156, 502, 511, 784, 1697
Hpy188I TCNGA 4 cut(s) 167, 1023, 1590, 1892
Hpy188III TCNNGA 7 cut(s) 127, 271, 478, 1229, 1595, 1635, 2135
Hpy8I GTNNAC 6 cut(s) 30, 156, 502, 511, 784, 1697
Hpy99I CGWCG 4 cut(s) 200, 616, 653, 1428
HpyCH4III ACNGT 3 cut(s) 34, 484, 957
HpyCH4IV ACGT 3 cut(s) 494, 549, 601
HpyF3I CTNAG 9 cut(s) 159, 675, 1208, 1280, 1367, 1721, 1889, 2021, 2117
HpySE526I ACGT 3 cut(s) 494, 549, 601
Hsp92I GRCGYC 4 cut(s) 201, 504, 651, 2008
HspAI GCGC 9 cut(s) 201, 331, 340, 360, 374, 490, 1708, 2008, 2073
KasI GGCGCC 2 cut(s) 200, 2007
KpnI GGTACC 1 cut(s) 1565
KroI GCCGGC 2 cut(s) 45, 1800
KroNI GCCGGC 2 cut(s) 47, 1802
Ksp22I TGATCA 3 cut(s) 708, 1491, 2128
KspI CCGCGG 1 cut(s) 433
Kzo9I GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
LguI GCTCTTC 3 cut(s) 410, 1143, 1301
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 9 cut(s) 179, 474, 482, 563, 1375, 1722, 1767, 1969, 2086
LweI GCATC 3 cut(s) 896, 1131, 1668
MaeI CTAG 7 cut(s) 242, 555, 749, 1152, 1250, 1635, 1769
MaeII ACGT 3 cut(s) 494, 549, 601
MalI GATC 9 cut(s) 140, 710, 1175, 1227, 1493, 1587, 1611, 2079, 2130
MbiI CCGCTC 2 cut(s) 63, 403
MboI GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
MfeI CAATTG 1 cut(s) 1966
MflI RGATCY 1 cut(s) 1173
MhlI GDGCHC 4 cut(s) 158, 308, 1138, 1281
MluCI AATT 9 cut(s) 704, 741, 767, 962, 973, 1650, 1919, 1966, 1997
MluI ACGCGT 1 cut(s) 150
Mly113I GGCGCC 2 cut(s) 201, 2008
MlyI GAGTC 5 cut(s) 433, 569, 1198, 1249, 1440
MmeI TCCRAC 2 cut(s) 33, 145
Mph1103I ATGCAT 2 cut(s) 1274, 1544
MroNI GCCGGC 2 cut(s) 45, 1800
MroXI GAANNNNTTC 2 cut(s) 1084, 1512
MseI TTAA 4 cut(s) 726, 1101, 1199, 1938
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 3 cut(s) 192, 432, 1366
MspCI CTTAAG 1 cut(s) 1937
MspI CCGG 9 cut(s) 46, 173, 259, 299, 668, 1028, 1758, 1801, 1912
MspR9I CCNGG 6 cut(s) 259, 299, 371, 542, 1758, 1789
MunI CAATTG 1 cut(s) 1966
MvaI CCWGG 3 cut(s) 371, 542, 1789
MvnI CGCG 8 cut(s) 152, 331, 342, 360, 376, 432, 1337, 1614
NaeI GCCGGC 2 cut(s) 47, 1802
NarI GGCGCC 2 cut(s) 201, 2008
NciI CCSGG 3 cut(s) 259, 299, 1758
NcoI CCATGG 1 cut(s) 1035
NdeI CATATG 1 cut(s) 1604
NdeII GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
NgoMIV GCCGGC 2 cut(s) 45, 1800
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 8 cut(s) 77, 478, 820, 1063, 1241, 1459, 1883, 2112
NsbI TGCGCA 1 cut(s) 491
NsiI ATGCAT 2 cut(s) 1274, 1544
NspI RCATGY 1 cut(s) 896
NspV TTCGAA 1 cut(s) 1516
OliI CACNNNNGTG 1 cut(s) 497
PceI AGGCCT 3 cut(s) 224, 1621, 1719
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 3 cut(s) 410, 1143, 1301
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PdiI GCCGGC 2 cut(s) 47, 1802
PdmI GAANNNNTTC 2 cut(s) 1084, 1512
PfeI GAWTC 4 cut(s) 1031, 1591, 1853, 2041
Pfl23II CGTACG 1 cut(s) 546
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 2 cut(s) 849, 1579
PfoI TCCNGGA 1 cut(s) 257
PleI GAGTC 5 cut(s) 433, 568, 1198, 1248, 1439
PluTI GGCGCC 2 cut(s) 204, 2011
PmaCI CACGTG 2 cut(s) 495, 602
PmlI CACGTG 2 cut(s) 495, 602
PpsI GAGTC 5 cut(s) 433, 568, 1198, 1248, 1439
Ppu21I YACGTR 2 cut(s) 495, 602
PpuMI RGGWCCY 2 cut(s) 319, 2033
PscI ACATGT 1 cut(s) 892
Psp5II RGGWCCY 2 cut(s) 319, 2033
Psp6I CCWGG 3 cut(s) 369, 540, 1787
PspCI CACGTG 2 cut(s) 495, 602
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 3 cut(s) 369, 540, 1787
PspLI CGTACG 1 cut(s) 546
PspOMI GGGCCC 1 cut(s) 304
PspPI GGNCC 9 cut(s) 71, 304, 305, 319, 385, 627, 1803, 2025, 2033
PspPPI RGGWCCY 2 cut(s) 319, 2033
PsuI RGATCY 1 cut(s) 1173
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 2 cut(s) 192, 1366
RsaI GTAC 6 cut(s) 548, 830, 918, 1563, 1916, 1995
RsaNI GTAC 6 cut(s) 547, 829, 917, 1562, 1915, 1994
RseI CAYNNNNRTG 1 cut(s) 497
SacII CCGCGG 1 cut(s) 433
SapI GCTCTTC 3 cut(s) 410, 1143, 1301
SaqAI TTAA 4 cut(s) 726, 1101, 1199, 1938
Sau3AI GATC 9 cut(s) 138, 708, 1173, 1225, 1491, 1585, 1609, 2077, 2128
Sau96I GGNCC 9 cut(s) 71, 304, 305, 319, 385, 627, 1803, 2025, 2033
SchI GAGTC 5 cut(s) 433, 569, 1198, 1249, 1440
ScrFI CCNGG 6 cut(s) 259, 299, 371, 542, 1758, 1789
SduI GDGCHC 4 cut(s) 158, 308, 1138, 1281
SfaNI GCATC 3 cut(s) 896, 1131, 1668
SfcI CTRYAG 1 cut(s) 2014
SfoI GGCGCC 2 cut(s) 202, 2009
Sfr303I CCGCGG 1 cut(s) 433
SfuI TTCGAA 1 cut(s) 1516
SgrBI CCGCGG 1 cut(s) 433
SinI GGWCC 4 cut(s) 319, 627, 2025, 2033
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 4 cut(s) 269, 632, 1937, 2135
SmoI CTYRAG 4 cut(s) 269, 632, 1937, 2135
Sse9I AATT 9 cut(s) 704, 741, 767, 962, 973, 1650, 1919, 1966, 1997
SseBI AGGCCT 3 cut(s) 224, 1621, 1719
SspDI GGCGCC 2 cut(s) 200, 2007
SspI AATATT 1 cut(s) 756
SspMI CTAG 7 cut(s) 242, 555, 749, 1152, 1250, 1635, 1769
StuI AGGCCT 3 cut(s) 224, 1621, 1719
StyD4I CCNGG 6 cut(s) 257, 297, 369, 540, 1756, 1787
StyI CCWWGG 3 cut(s) 23, 1035, 1714
TaaI ACNGT 3 cut(s) 34, 484, 957
TaiI ACGT 3 cut(s) 497, 552, 604
TaqI TCGA 7 cut(s) 128, 141, 185, 324, 396, 648, 1516
TaqII GACCGA 1 cut(s) 615
TasI AATT 9 cut(s) 704, 741, 767, 962, 973, 1650, 1919, 1966, 1997
TatI WGTACW 2 cut(s) 916, 1993
TauI GCSGC 8 cut(s) 66, 69, 72, 288, 432, 465, 2013, 2125
TfiI GAWTC 4 cut(s) 1031, 1591, 1853, 2041
Tru1I TTAA 4 cut(s) 726, 1101, 1199, 1938
Tru9I TTAA 4 cut(s) 726, 1101, 1199, 1938
TscAI CASTG 5 cut(s) 829, 962, 1136, 1250, 1288
TseFI GTSAC 8 cut(s) 77, 478, 820, 1063, 1241, 1459, 1883, 2112
TseI GCWGC 9 cut(s) 192, 470, 487, 576, 1363, 1710, 1780, 1982, 2099
Tsp45I GTSAC 8 cut(s) 77, 478, 820, 1063, 1241, 1459, 1883, 2112
TspDTI ATGAA 6 cut(s) 871, 1458, 1656, 1675, 1798, 2098
TspRI CASTG 5 cut(s) 829, 962, 1136, 1250, 1288
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 2 cut(s) 849, 1579
Vha464I CTTAAG 1 cut(s) 1937
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 4 cut(s) 319, 627, 2025, 2033
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 1919
XbaI TCTAGA 1 cut(s) 1634
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 1242
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 2 cut(s) 1084, 1512
XspI CTAG 7 cut(s) 242, 555, 749, 1152, 1250, 1635, 1769
Zsp2I ATGCAT 2 cut(s) 1274, 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.